CSPI04G12550.1 (mRNA) Wild cucumber (PI 183967)

NameCSPI04G12550.1
TypemRNA
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionMitochondrial oxoglutarate/malate carrier protein, putative
LocationChr4 : 10948191 .. 10952192 (-)
Sequence length900
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAGAGGTTTGAGGCTGAGGCGCGAAGCAAAAGGAAGATATAAAAAGAAGTTGGGCCCTTTATTTTCGGAGAAGGGAATCCACCACCTCCATCTCAACTCCCTCTGCTCTCACCAATGGCTGACGATAAGAAACCCAGCGTCTCCCCCGTTTGGCCCACCGTCAAGCCCTTTGTTAATGGCGGTGCCTCCGGTATGCTCGCCACCTGCGTCATTCAACCCATTGATATGGTCAAGGTACTCTACTTCCATCAAATGCCAATGCCCTTATCTTCAATTCCTGCTGGCTGCTTCTAATCTTTATCTACTGCTCCGCTTTCTCTTAGGTCAGAATTCAACTTGGTCAAGGATCCGCTGGCCATGTCACAAGAACCATGCTTAAGGAGGAGGGTTTTGGTGCCTTTTACAAGGTTTCCATTTTTCATTCTGCTCAGCAAACAAATTATTTCCTTCCTTGTTTTTATGCTACTACCCCTCCCCTGTTTCCTTTCTTTCCTCATACCGAATTGTTGTATCTTTTGTTGAACTACGATGGTATTGCATTACTGTCTCCGATCATTGTGTTCTGCATATCTTTTCGATGTGTTCTACTGAATTATTTATTTGTTGCCACCATTGAGTGACTGATGATGTATTAACTGAGGAGCCACTTGAAAATTAAGTGGTTGTTTGACACATTTTCAGTATCTTGTAAATTAGGATATAGGCAAAGTTCATTTTCTAATGGATGAAGATTTGCTTGGATTTTTTTCCTTTCAAAATTTTCCGGTTCTCTAGTGCTCGACACTTAGTTTTGTGGATTATCTTTGTCTAAACTTCATTTTCCAACCAGGGGTTATCTGCTGGATTGCTCAGACAAGCTACATATACTACAGCAAGACTGGGATCTTTTAAGTGAGCGTGTTACAAATCTAGAATTGAACACCTTTGGATCATATTCATGAGGCTATGGTTTTACTTCCAACATTTGTTCTTCCTCATGTCTATGTAGTTTTTTTGAGAACTTTAAGAAAATTTGCATTTCTTAGTCCCTACATTTTAGGAATGATCAATTGGTTCCTATAGTTTGAAACATGACAATCTAGTTACTGTAGGATTAAATGTGGTTCTCAATTGCCCTCAAATTATTTTATCCAGTGGGTCCCATGAAAATGTCACATGATCTTATAGTTTATGTTAGTTAAGTTCGTAGGTGCCAAGTCATTTGTATTTGAGACTCATCATTAAGTCAAAACCACACAGATATAATGACCATCTAAGGCTACTGGAGTTAAATCGTCACATTCTGAACTACAAGGACCATGTAGAATCATTTCCTGGACTGTAGTGGCTAAATGTTTTTTTTTTTTTTTGGCTTGTATATTTGGTTGATCAAACCTCTGTTCTTCTCCATTTCCCTTCTAGGATATTGACAAACAAAGCAATTGAAGCCAACGAGGGAAAGCCCCTTCCACTATATCAGAAGGCCTTATGTGGGCTCACAGCCGGTGCTATTGGAGCATCTGTTGGTAGTCCAGCGGACTTAGCTCTTATTCGCATGCAAGCAGATGCAACTTTACCTGCTGCCCAGCGCCGAAATTATAAAAATGCCTTCCATGCACTTTATCGCATTCTTGCAGATGAGGGGGTTTTAGCTCTTTGGAAAGGTGCAGGCCCCACTATTGTAAGAGCAATGGGATTGAACATGGGAATGCTTGCTTCATATGATCAAAGTGTTGAGTTCTTCAAGGATAACCTTGGTTTTGGCGAAGCTGCTACGGTGTTGGGTAAGATTGTTTTTGATACTGTAAGTTGATGCCAATTGTAATGCCCCATGCGGTGTTGCACTAATTGCTAGGGTATTGCAATTTCATCGGCTCATTACTATCTCTTTCTTAGTAGTCATCTAGTCATTTCATATTCACGTGTGTATAATTTGACACTGTTGAAAATAATTAGTTTAACTAACTTAATCATAATCATTGTTGGTCAAGATTAGCTGCCATCTTTTCATTGAGGTTTCTATGGAATAATAGATAATTTTGACTTTTGAGAGAGGTCAAACTTTAAGTTTGATTAATAAACTCGCCCTTGGAGTTGTACTTTATTTCAATGAGAGGATCTAAATGTTTTTTTTCGTATTCCTGTATCGATTCAGTTCGTAGATACTAGATTTATGACTAGGTAAGCTTGTTATATTTGTTATCCACAGATATTCTTTTGCTGTTCTTACCGTAGAGAGTTGAATTTACCACACTTTTCTTCAGTATGAGCAGCTTAGCTTATATTGCTTAAGAGGCAAGTGGCCACTGAACGTTGAGTGGAATTGCTCAAAGTTTTCACTTATCGTTGGATTGTTATAAGTTCAATGAAAGTTCTTCCTTTGTCTTTCTCCTTCGTTCTTCTGTCTGTGTGTATTAAAATGGTGGTCTTTGGAAATGTTTCTATTGTGAGTCGCCTTCTCTCTGATTGTGATTCTTGCTAAATTTCTTTTATGATTTATTATTTATTGTTCTTCTTAGGTGCCAGTATGGTATCAGGATTCTTTGCTTCAGCGTGTAGTCTACCATTTGATTATGTGAAAACTCAAATTCAGAAAATGCAACCCGATGCTGAGGGAAAATTTCCCTACTCTGGCTCTTTGGATTGTGCCATGAAAACTCTCAAGGCAGGAGGGCCGTTAAAATTCTACACTGGATTTCCAGTGTATTGCGTGAGAATTGCCCCCCATGTCATGGTATATCTCTTTTCTCTGTCTGTCTGTCTGTGCACACACTACTTGGGTTATCATGGTGCCTATCGCTGTTGTAGCTGGGTATCTATCAATGGCGATACCACATTTAAGATAATTCACTGAATCAAATGTTGGACTTGATGATGCAGATGACTTGGATTTTCCTAAACCAGATTCAAAAGGTAGAAAAATCATACGGGTTATGAAGGACTGACCAAGATGATGGAGATCCTTTCCAAGTTTATTTCAGGCTTCAACAATTATGATTTTTTTTTCCTTGTGGTTCTTTGACCATGTTGTAATCTAACTTTCTTTGAATACGGAATTCCACGCTTGAGTTTAAAAGCGTTTTAATTATCTTTCTGTGCAAACATGGTTCAAAGGTGCAAGATTGATAGATAGAAAGAGGGTATCAAATTGCTTCTGAAATTTGAATGTACCAGATTCTCACCGGGAAGAAACATAGAATACATACATAATAAGCCTTGTAAAGAGTTCTTTAAAGGTTAAACTCTGTCCCAACCACAACATTCTCGTGGAGGCATTTGAATGATTAGAAATTAACGTATGGAGGATGCTCCTGATCTGCTAATGTAAATAGTTCATGTTTGCTTTGGCGATTTGACAATAAAATATTGGTAAGGTGTTTATTTTCAGGCTAAGACAATCACATCAGCATTTATAAGAAAGAATGTAGGTTCATTAGAGGAAATAATGAAATGGCAGACAATATACAATCAGAAAAGCAATGCGAAGAAGTTTAAAGGAAGAAGGAAAAAAAAAGAGCAGAAAGAGAAGCAAGGAAAGTAGGGTAAAAGAGAGGAGCATCCGAGGCAGGAGCAGGAGCAGGAGCAGGAGCAGCAGGCTGGGCACTGGTGGCAGCGAAGGCCAGCAGCAGCAGCACCAGGAACAGCTTCAACTTGATTGCCTCCATTGCTGAATGATACAGCTCTTGTCTTGTTGAATCCAATTGAATTGAAGAAGATGAAGAGTTGTTGGGGGCGTGAATTGGGAAGGAAGAAGAGACTATTTATGTATGAAAAATGCATTCAGGAACTCGAGAGGCTGAGACAGAGACAGGAACACAACAGGGTGGTTTTGGTTGGTTGCTTTTATCTTACCATACGCTAATTGATACAGCTAACTCTCAGTGGCTGTGGGTCCCCGTGTTTTGCTGTCTTTCTGTATTTTTCTCTCTCTTTTTTCTTTTCTTTTTTCTATGATTTTCTGTTTGAAAAAGACTTGTGTACGTCCCGATGATGCATCCATCTCATGTATTTTTCATCTCTTC

mRNA sequence

ATGGCTGACGATAAGAAACCCAGCGTCTCCCCCGTTTGGCCCACCGTCAAGCCCTTTGTTAATGGCGGTGCCTCCGGTATGCTCGCCACCTGCGTCATTCAACCCATTGATATGGTCAAGGTCAGAATTCAACTTGGTCAAGGATCCGCTGGCCATGTCACAAGAACCATGCTTAAGGAGGAGGGTTTTGGTGCCTTTTACAAGGGGTTATCTGCTGGATTGCTCAGACAAGCTACATATACTACAGCAAGACTGGGATCTTTTAAGATATTGACAAACAAAGCAATTGAAGCCAACGAGGGAAAGCCCCTTCCACTATATCAGAAGGCCTTATGTGGGCTCACAGCCGGTGCTATTGGAGCATCTGTTGGTAGTCCAGCGGACTTAGCTCTTATTCGCATGCAAGCAGATGCAACTTTACCTGCTGCCCAGCGCCGAAATTATAAAAATGCCTTCCATGCACTTTATCGCATTCTTGCAGATGAGGGGGTTTTAGCTCTTTGGAAAGGTGCAGGCCCCACTATTGTAAGAGCAATGGGATTGAACATGGGAATGCTTGCTTCATATGATCAAAGTGTTGAGTTCTTCAAGGATAACCTTGGTTTTGGCGAAGCTGCTACGGTGTTGGGTGCCAGTATGGTATCAGGATTCTTTGCTTCAGCGTGTAGTCTACCATTTGATTATGTGAAAACTCAAATTCAGAAAATGCAACCCGATGCTGAGGGAAAATTTCCCTACTCTGGCTCTTTGGATTGTGCCATGAAAACTCTCAAGGCAGGAGGGCCGTTAAAATTCTACACTGGATTTCCAGTGTATTGCGTGAGAATTGCCCCCCATGTCATGATGACTTGGATTTTCCTAAACCAGATTCAAAAGGTAGAAAAATCATACGGGTTATGA

Coding sequence (CDS)

ATGGCTGACGATAAGAAACCCAGCGTCTCCCCCGTTTGGCCCACCGTCAAGCCCTTTGTTAATGGCGGTGCCTCCGGTATGCTCGCCACCTGCGTCATTCAACCCATTGATATGGTCAAGGTCAGAATTCAACTTGGTCAAGGATCCGCTGGCCATGTCACAAGAACCATGCTTAAGGAGGAGGGTTTTGGTGCCTTTTACAAGGGGTTATCTGCTGGATTGCTCAGACAAGCTACATATACTACAGCAAGACTGGGATCTTTTAAGATATTGACAAACAAAGCAATTGAAGCCAACGAGGGAAAGCCCCTTCCACTATATCAGAAGGCCTTATGTGGGCTCACAGCCGGTGCTATTGGAGCATCTGTTGGTAGTCCAGCGGACTTAGCTCTTATTCGCATGCAAGCAGATGCAACTTTACCTGCTGCCCAGCGCCGAAATTATAAAAATGCCTTCCATGCACTTTATCGCATTCTTGCAGATGAGGGGGTTTTAGCTCTTTGGAAAGGTGCAGGCCCCACTATTGTAAGAGCAATGGGATTGAACATGGGAATGCTTGCTTCATATGATCAAAGTGTTGAGTTCTTCAAGGATAACCTTGGTTTTGGCGAAGCTGCTACGGTGTTGGGTGCCAGTATGGTATCAGGATTCTTTGCTTCAGCGTGTAGTCTACCATTTGATTATGTGAAAACTCAAATTCAGAAAATGCAACCCGATGCTGAGGGAAAATTTCCCTACTCTGGCTCTTTGGATTGTGCCATGAAAACTCTCAAGGCAGGAGGGCCGTTAAAATTCTACACTGGATTTCCAGTGTATTGCGTGAGAATTGCCCCCCATGTCATGATGACTTGGATTTTCCTAAACCAGATTCAAAAGGTAGAAAAATCATACGGGTTATGA
BLAST of CSPI04G12550.1 vs. Swiss-Prot
Match: DTC_ARATH (Mitochondrial dicarboxylate/tricarboxylate transporter DTC OS=Arabidopsis thaliana GN=DTC PE=1 SV=1)

HSP 1 Score: 508.4 bits (1308), Expect = 5.2e-143
Identity = 249/299 (83.28%), Postives = 270/299 (90.30%), Query Frame = 1

Query: 1   MADDKKPSVSPVWPTVKPFVNGGASGMLATCVIQPIDMVKVRIQLGQGSAGHVTRTMLKE 60
           MA++KK  +S VW TVKPFVNGGASGMLATCVIQPIDM+KVRIQLGQGSA  +T  MLK 
Sbjct: 1   MAEEKKAPIS-VWTTVKPFVNGGASGMLATCVIQPIDMIKVRIQLGQGSAASITTNMLKN 60

Query: 61  EGFGAFYKGLSAGLLRQATYTTARLGSFKILTNKAIEANEGKPLPLYQKALCGLTAGAIG 120
           EG GAFYKGLSAGLLRQATYTTARLGSFK+LT KAIE+N+GKPLPLYQKALCGLTAGAIG
Sbjct: 61  EGVGAFYKGLSAGLLRQATYTTARLGSFKLLTAKAIESNDGKPLPLYQKALCGLTAGAIG 120

Query: 121 ASVGSPADLALIRMQADATLPAAQRRNYKNAFHALYRILADEGVLALWKGAGPTIVRAMG 180
           A VGSPADLALIRMQAD TLP AQRRNY NAFHAL RI ADEGVLALWKG GPT+VRAM 
Sbjct: 121 ACVGSPADLALIRMQADNTLPLAQRRNYTNAFHALTRISADEGVLALWKGCGPTVVRAMA 180

Query: 181 LNMGMLASYDQSVEFFKDNLGFGEAATVLGASMVSGFFASACSLPFDYVKTQIQKMQPDA 240
           LNMGMLASYDQS E+ +DNLGFGE +TV+GAS VSGF A+ACSLPFD+VKTQIQKMQPDA
Sbjct: 181 LNMGMLASYDQSAEYMRDNLGFGEMSTVVGASAVSGFCAAACSLPFDFVKTQIQKMQPDA 240

Query: 241 EGKFPYSGSLDCAMKTLKAGGPLKFYTGFPVYCVRIAPHVMMTWIFLNQIQKVEKSYGL 300
           +GK+PY+GSLDCAMKTLK GGPLKFY+GFPVYCVRIAPHVMMTWIFLNQI K +K  G+
Sbjct: 241 QGKYPYTGSLDCAMKTLKEGGPLKFYSGFPVYCVRIAPHVMMTWIFLNQITKFQKKIGM 298

BLAST of CSPI04G12550.1 vs. Swiss-Prot
Match: M2OM_NEUCR (Putative mitochondrial 2-oxoglutarate/malate carrier protein OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) GN=mic-33 PE=3 SV=1)

HSP 1 Score: 256.5 bits (654), Expect = 3.5e-67
Identity = 139/284 (48.94%), Postives = 177/284 (62.32%), Query Frame = 1

Query: 10  SPVWPTVKPFVNGGASGMLATCVIQPIDMVKVRIQL-GQGSAGH-------VTRTMLKEE 69
           +P      PF+NGG SGM+AT VIQPIDM+KVRIQL G+G AG        VTR ++   
Sbjct: 36  TPAVRAALPFINGGLSGMVATTVIQPIDMIKVRIQLAGEGKAGGPKPTPLGVTRDIIASG 95

Query: 70  GFGAFYKGLSAGLLRQATYTTARLGSFKILTNK--AIEANEGKPLPLYQKALCGLTAGAI 129
                Y GLSAGLLRQA YTTAR+G F    ++  A    +G+ +   ++A  GL AG +
Sbjct: 96  KAMDLYTGLSAGLLRQAVYTTARIGCFDTFMSRLSARAKEKGQSVGFKERASAGLAAGGL 155

Query: 130 GASVGSPADLALIRMQADATLPAAQRRNYKNAFHALYRILADEGVLALWKGAGPTIVRAM 189
            A +G+PADLALIRMQ+D   P A+R+NYK+   AL  I  +EGV ALW GA PT+VRAM
Sbjct: 156 AAMIGNPADLALIRMQSDGLKPVAERKNYKSVIDALGGIARNEGVAALWAGAAPTVVRAM 215

Query: 190 GLNMGMLASYDQSVEFFKDNLGFGEAATVLGASMVSGFFASACSLPFDYVKTQIQKMQPD 249
            LN G LA + ++    K    +      L AS ++GFFAS  SLPFD+VKT++QK    
Sbjct: 216 ALNFGQLAFFSEAKAQLKARTQWSSKVQTLSASAIAGFFASFFSLPFDFVKTRLQKQTRG 275

Query: 250 AEGKFPYSGSLDCAMKTLKAGGPLKFYTGFPVYCVRIAPHVMMT 284
            +GK PY+G +DC  K  K  G  +FY GF  Y VRIAPH M+T
Sbjct: 276 PDGKLPYNGMVDCFAKVAKQEGVFRFYRGFGTYYVRIAPHAMVT 319

BLAST of CSPI04G12550.1 vs. Swiss-Prot
Match: M2OM_MOUSE (Mitochondrial 2-oxoglutarate/malate carrier protein OS=Mus musculus GN=Slc25a11 PE=1 SV=3)

HSP 1 Score: 236.9 bits (603), Expect = 2.9e-61
Identity = 141/301 (46.84%), Postives = 185/301 (61.46%), Query Frame = 1

Query: 4   DKKPSVSPVWPTVKPFVNGGASGMLATCVIQPIDMVKVRIQL-GQGS-------AGHVTR 63
           D KP  SP   +VK F+ GG +GM AT  +QP+D+VK R+QL G+G+       + H   
Sbjct: 13  DGKPRTSP--KSVK-FLFGGLAGMGATVFVQPLDLVKNRMQLSGEGAKTREYKTSFHALT 72

Query: 64  TMLKEEGFGAFYKGLSAGLLRQATYTTARLGSFKILTNKAIEANEGKPLPLYQKALCGLT 123
           ++LK EG    Y GLSAGLLRQATYTT RLG + +L  +   A +G P     KAL G+T
Sbjct: 73  SILKTEGLKGIYTGLSAGLLRQATYTTTRLGIYTVLFERLTGA-DGTPPGFLLKALIGMT 132

Query: 124 AGAIGASVGSPADLALIRMQADATLPAAQRRNYKNAFHALYRILADEGVLALWKGAGPTI 183
           AGA GA VG+PA++ALIRM AD  LPA QRR YKN F+AL RI  +EGV  LW+G  PT+
Sbjct: 133 AGATGAFVGTPAEVALIRMTADGRLPADQRRGYKNVFNALVRIAREEGVPTLWRGCIPTM 192

Query: 184 VRAMGLNMGMLASYDQSVEFFKDNLGFGEAATV-LGASMVSGFFASACSLPFDYVKTQIQ 243
            RA+ +N   LASY QS +F  D+  F +       ASM+SG   +A S+P D VKT+IQ
Sbjct: 193 ARAVVVNAAQLASYSQSKQFLLDSGYFSDNILCHFCASMISGLVTTAASMPVDIVKTRIQ 252

Query: 244 KMQPDAEGKFPYSGSLDCAMKTLKAGGPLKFYTGFPVYCVRIAPHVMMTWIFLNQIQKVE 296
            M+   +GK  Y   LD  +K ++  G    + GF  Y  R+ PH ++T+IFL Q+ K  
Sbjct: 253 NMRM-IDGKPEYKNGLDVLLKVVRYEGFFSLWKGFTPYYARLGPHTVLTFIFLEQMNKAY 308

BLAST of CSPI04G12550.1 vs. Swiss-Prot
Match: M2OM_HUMAN (Mitochondrial 2-oxoglutarate/malate carrier protein OS=Homo sapiens GN=SLC25A11 PE=1 SV=3)

HSP 1 Score: 236.1 bits (601), Expect = 5.0e-61
Identity = 140/301 (46.51%), Postives = 184/301 (61.13%), Query Frame = 1

Query: 4   DKKPSVSPVWPTVKPFVNGGASGMLATCVIQPIDMVKVRIQL-GQGS-------AGHVTR 63
           D KP  SP   +VK F+ GG +GM AT  +QP+D+VK R+QL G+G+       + H   
Sbjct: 13  DGKPRTSP--KSVK-FLFGGLAGMGATVFVQPLDLVKNRMQLSGEGAKTREYKTSFHALT 72

Query: 64  TMLKEEGFGAFYKGLSAGLLRQATYTTARLGSFKILTNKAIEANEGKPLPLYQKALCGLT 123
           ++LK EG    Y GLSAGLLRQATYTT RLG + +L  +   A +G P     KA+ G+T
Sbjct: 73  SILKAEGLRGIYTGLSAGLLRQATYTTTRLGIYTVLFERLTGA-DGTPPGFLLKAVIGMT 132

Query: 124 AGAIGASVGSPADLALIRMQADATLPAAQRRNYKNAFHALYRILADEGVLALWKGAGPTI 183
           AGA GA VG+PA++ALIRM AD  LPA QRR YKN F+AL RI  +EGVL LW+G  PT+
Sbjct: 133 AGATGAFVGTPAEVALIRMTADGRLPADQRRGYKNVFNALIRITREEGVLTLWRGCIPTM 192

Query: 184 VRAMGLNMGMLASYDQSVEFFKDNLGFGEAATV-LGASMVSGFFASACSLPFDYVKTQIQ 243
            RA+ +N   LASY QS +F  D+  F +       ASM+SG   +A S+P D  KT+IQ
Sbjct: 193 ARAVVVNAAQLASYSQSKQFLLDSGYFSDNILCHFCASMISGLVTTAASMPVDIAKTRIQ 252

Query: 244 KMQPDAEGKFPYSGSLDCAMKTLKAGGPLKFYTGFPVYCVRIAPHVMMTWIFLNQIQKVE 296
            M+   +GK  Y   LD   K ++  G    + GF  Y  R+ PH ++T+IFL Q+ K  
Sbjct: 253 NMRM-IDGKPEYKNGLDVLFKVVRYEGFFSLWKGFTPYYARLGPHTVLTFIFLEQMNKAY 308

BLAST of CSPI04G12550.1 vs. Swiss-Prot
Match: M2OM_BOVIN (Mitochondrial 2-oxoglutarate/malate carrier protein OS=Bos taurus GN=SLC25A11 PE=1 SV=3)

HSP 1 Score: 234.2 bits (596), Expect = 1.9e-60
Identity = 138/301 (45.85%), Postives = 186/301 (61.79%), Query Frame = 1

Query: 4   DKKPSVSPVWPTVKPFVNGGASGMLATCVIQPIDMVKVRIQL-GQGS-------AGHVTR 63
           D KP  SP   +VK F+ GG +GM AT  +QP+D+VK R+QL G+G+       + H   
Sbjct: 13  DGKPRTSP--KSVK-FLFGGLAGMGATVFVQPLDLVKNRMQLSGEGAKTREYKTSFHALI 72

Query: 64  TMLKEEGFGAFYKGLSAGLLRQATYTTARLGSFKILTNKAIEANEGKPLPLYQKALCGLT 123
           ++L+ EG    Y GLSAGLLRQATYTT RLG + +L  +   A +G P     KA+ G+T
Sbjct: 73  SILRAEGLRGIYTGLSAGLLRQATYTTTRLGIYTVLFERLTGA-DGTPPGFLLKAVIGMT 132

Query: 124 AGAIGASVGSPADLALIRMQADATLPAAQRRNYKNAFHALYRILADEGVLALWKGAGPTI 183
           AGA GA VG+PA++ALIRM AD  LP  QRR YKN F+AL+RI+ +EGV  LW+G  PT+
Sbjct: 133 AGATGAFVGTPAEVALIRMTADGRLPVDQRRGYKNVFNALFRIVQEEGVPTLWRGCIPTM 192

Query: 184 VRAMGLNMGMLASYDQSVEFFKDNLGFGEAATV-LGASMVSGFFASACSLPFDYVKTQIQ 243
            RA+ +N   LASY QS +F  D+  F +       ASM+SG   +A S+P D VKT+IQ
Sbjct: 193 ARAVVVNAAQLASYSQSKQFLLDSGYFSDNILCHFCASMISGLVTTAASMPVDIVKTRIQ 252

Query: 244 KMQPDAEGKFPYSGSLDCAMKTLKAGGPLKFYTGFPVYCVRIAPHVMMTWIFLNQIQKVE 296
            M+   +GK  Y   LD  +K ++  G    + GF  Y  R+ PH ++T+IFL Q+ K  
Sbjct: 253 NMRM-IDGKPEYKNGLDVLVKVVRYEGFFSLWKGFTPYYARLGPHTVLTFIFLEQMNKAY 308

BLAST of CSPI04G12550.1 vs. TrEMBL
Match: A0A0A0KZ03_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G268080 PE=3 SV=1)

HSP 1 Score: 601.3 bits (1549), Expect = 6.5e-169
Identity = 298/299 (99.67%), Postives = 299/299 (100.00%), Query Frame = 1

Query: 1   MADDKKPSVSPVWPTVKPFVNGGASGMLATCVIQPIDMVKVRIQLGQGSAGHVTRTMLKE 60
           MADDKKPSVSPVWPTVKPFVNGGASGMLATCVIQPIDMVKVRIQLGQGSAGHVTRTMLKE
Sbjct: 1   MADDKKPSVSPVWPTVKPFVNGGASGMLATCVIQPIDMVKVRIQLGQGSAGHVTRTMLKE 60

Query: 61  EGFGAFYKGLSAGLLRQATYTTARLGSFKILTNKAIEANEGKPLPLYQKALCGLTAGAIG 120
           EGFGAFYKGLSAGLLRQATYTTARLGSFKILTNKAIEANEGKPLPLYQKALCGLTAGAIG
Sbjct: 61  EGFGAFYKGLSAGLLRQATYTTARLGSFKILTNKAIEANEGKPLPLYQKALCGLTAGAIG 120

Query: 121 ASVGSPADLALIRMQADATLPAAQRRNYKNAFHALYRILADEGVLALWKGAGPTIVRAMG 180
           ASVGSPADLALIRMQADATLPAAQRRNYKNAFHALYRILADEGVLALWKGAGPTIVRAMG
Sbjct: 121 ASVGSPADLALIRMQADATLPAAQRRNYKNAFHALYRILADEGVLALWKGAGPTIVRAMG 180

Query: 181 LNMGMLASYDQSVEFFKDNLGFGEAATVLGASMVSGFFASACSLPFDYVKTQIQKMQPDA 240
           LNMGMLASYDQSVEFFKDNLGFGEAATVLGASMVSGFFASACSLPFDYVKTQIQKMQPDA
Sbjct: 181 LNMGMLASYDQSVEFFKDNLGFGEAATVLGASMVSGFFASACSLPFDYVKTQIQKMQPDA 240

Query: 241 EGKFPYSGSLDCAMKTLKAGGPLKFYTGFPVYCVRIAPHVMMTWIFLNQIQKVEKSYGL 300
           EGKFP+SGSLDCAMKTLKAGGPLKFYTGFPVYCVRIAPHVMMTWIFLNQIQKVEKSYGL
Sbjct: 241 EGKFPHSGSLDCAMKTLKAGGPLKFYTGFPVYCVRIAPHVMMTWIFLNQIQKVEKSYGL 299

BLAST of CSPI04G12550.1 vs. TrEMBL
Match: A0A067LC67_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_01873 PE=3 SV=1)

HSP 1 Score: 553.9 bits (1426), Expect = 1.2e-154
Identity = 269/299 (89.97%), Postives = 286/299 (95.65%), Query Frame = 1

Query: 1   MADDKKPSVSPVWPTVKPFVNGGASGMLATCVIQPIDMVKVRIQLGQGSAGHVTRTMLKE 60
           M ++KK   + VWPTVKPFVNGGASGMLATCVIQPIDM+KVRIQLGQGSA  VT+TMLKE
Sbjct: 1   MGEEKKAQPAGVWPTVKPFVNGGASGMLATCVIQPIDMIKVRIQLGQGSAAQVTKTMLKE 60

Query: 61  EGFGAFYKGLSAGLLRQATYTTARLGSFKILTNKAIEANEGKPLPLYQKALCGLTAGAIG 120
           EGFGAFYKGLSAGLLRQATYTTARLGSF++LTNKAIEAN+GKPLPLYQKALCGLTAGAIG
Sbjct: 61  EGFGAFYKGLSAGLLRQATYTTARLGSFRMLTNKAIEANDGKPLPLYQKALCGLTAGAIG 120

Query: 121 ASVGSPADLALIRMQADATLPAAQRRNYKNAFHALYRILADEGVLALWKGAGPTIVRAMG 180
           A VGSPADLALIRMQADATLPAAQRRNY NAFHALYRI+ADEGVLALWKGAGPT+VRAM 
Sbjct: 121 ACVGSPADLALIRMQADATLPAAQRRNYTNAFHALYRIVADEGVLALWKGAGPTVVRAMA 180

Query: 181 LNMGMLASYDQSVEFFKDNLGFGEAATVLGASMVSGFFASACSLPFDYVKTQIQKMQPDA 240
           LNMGMLASYDQSVEFF+DNLGFGEAATV+GAS VSGFFASACSLPFDYVKTQIQKMQPDA
Sbjct: 181 LNMGMLASYDQSVEFFRDNLGFGEAATVIGASSVSGFFASACSLPFDYVKTQIQKMQPDA 240

Query: 241 EGKFPYSGSLDCAMKTLKAGGPLKFYTGFPVYCVRIAPHVMMTWIFLNQIQKVEKSYGL 300
           +GK+PY+GSLDCAMKTLKAGGP KFYTGFPVYCVRIAPHVMMTWIFLNQIQK+EK+ GL
Sbjct: 241 QGKYPYTGSLDCAMKTLKAGGPFKFYTGFPVYCVRIAPHVMMTWIFLNQIQKLEKNVGL 299

BLAST of CSPI04G12550.1 vs. TrEMBL
Match: W9SZF7_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_019087 PE=3 SV=1)

HSP 1 Score: 552.7 bits (1423), Expect = 2.7e-154
Identity = 268/299 (89.63%), Postives = 285/299 (95.32%), Query Frame = 1

Query: 1   MADDKKPSVSPVWPTVKPFVNGGASGMLATCVIQPIDMVKVRIQLGQGSAGHVTRTMLKE 60
           M D+KKP  + VWPTVKPFVNGGASGMLATCVIQPIDM+KVRIQLGQGSAG VT+TMLKE
Sbjct: 1   MGDEKKPKSTGVWPTVKPFVNGGASGMLATCVIQPIDMIKVRIQLGQGSAGQVTKTMLKE 60

Query: 61  EGFGAFYKGLSAGLLRQATYTTARLGSFKILTNKAIEANEGKPLPLYQKALCGLTAGAIG 120
           EG GAFYKGLSAGLLRQATYTTARLGSFKILTNKAIEAN+GKPLPLYQKALCGLTAGAIG
Sbjct: 61  EGVGAFYKGLSAGLLRQATYTTARLGSFKILTNKAIEANDGKPLPLYQKALCGLTAGAIG 120

Query: 121 ASVGSPADLALIRMQADATLPAAQRRNYKNAFHALYRILADEGVLALWKGAGPTIVRAMG 180
           ASVGSPADLALIRMQADATLP AQRRNYKNAFHALYRI++DEGVLALWKGAGPT+VRAM 
Sbjct: 121 ASVGSPADLALIRMQADATLPVAQRRNYKNAFHALYRIVSDEGVLALWKGAGPTVVRAMA 180

Query: 181 LNMGMLASYDQSVEFFKDNLGFGEAATVLGASMVSGFFASACSLPFDYVKTQIQKMQPDA 240
           LNMGMLASYDQSVEFFKD++G GEA+TVL AS VSGFFA+ACSLPFDYVKTQIQKMQPDA
Sbjct: 181 LNMGMLASYDQSVEFFKDSVGLGEASTVLAASSVSGFFAAACSLPFDYVKTQIQKMQPDA 240

Query: 241 EGKFPYSGSLDCAMKTLKAGGPLKFYTGFPVYCVRIAPHVMMTWIFLNQIQKVEKSYGL 300
           +GK+PY+GSLDCA+KTLKAGGP KFYTGFPVYCVRIAPHVMMTWIFLNQ+QK EKS GL
Sbjct: 241 QGKYPYTGSLDCALKTLKAGGPFKFYTGFPVYCVRIAPHVMMTWIFLNQVQKFEKSIGL 299

BLAST of CSPI04G12550.1 vs. TrEMBL
Match: A0A059D9A4_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_B03603 PE=3 SV=1)

HSP 1 Score: 551.6 bits (1420), Expect = 5.9e-154
Identity = 270/299 (90.30%), Postives = 283/299 (94.65%), Query Frame = 1

Query: 1   MADDKKPSVSPVWPTVKPFVNGGASGMLATCVIQPIDMVKVRIQLGQGSAGHVTRTMLKE 60
           M ++KKP  + VWPTVKPFVNGGASGMLATCVIQPIDM+KVRIQLGQGSAG VTR MLKE
Sbjct: 1   MGEEKKPKPAGVWPTVKPFVNGGASGMLATCVIQPIDMIKVRIQLGQGSAGQVTRNMLKE 60

Query: 61  EGFGAFYKGLSAGLLRQATYTTARLGSFKILTNKAIEANEGKPLPLYQKALCGLTAGAIG 120
           EG GAFYKGLSAGLLRQATYTTARLGSF+ILT KAIEAN+GKPLPLYQKALCGLTAGAIG
Sbjct: 61  EGMGAFYKGLSAGLLRQATYTTARLGSFRILTAKAIEANDGKPLPLYQKALCGLTAGAIG 120

Query: 121 ASVGSPADLALIRMQADATLPAAQRRNYKNAFHALYRILADEGVLALWKGAGPTIVRAMG 180
           ASVGSPADLALIRMQADATLPAAQRR+Y NAFHALYRI+ DEGVLALWKGAGPT+VRAM 
Sbjct: 121 ASVGSPADLALIRMQADATLPAAQRRHYTNAFHALYRIVGDEGVLALWKGAGPTVVRAMA 180

Query: 181 LNMGMLASYDQSVEFFKDNLGFGEAATVLGASMVSGFFASACSLPFDYVKTQIQKMQPDA 240
           LNMGMLASYDQ VEFFKD+LGFGE ATVLGAS VSGFFA+ACSLPFDYVKTQIQKMQPDA
Sbjct: 181 LNMGMLASYDQGVEFFKDSLGFGEGATVLGASTVSGFFAAACSLPFDYVKTQIQKMQPDA 240

Query: 241 EGKFPYSGSLDCAMKTLKAGGPLKFYTGFPVYCVRIAPHVMMTWIFLNQIQKVEKSYGL 300
           EGK+PY+GSLDCAMKTLKAGGPLKFYTGFPVYCVRIAPHVMMTWIFLNQIQK EKS GL
Sbjct: 241 EGKYPYTGSLDCAMKTLKAGGPLKFYTGFPVYCVRIAPHVMMTWIFLNQIQKFEKSVGL 299

BLAST of CSPI04G12550.1 vs. TrEMBL
Match: B9T1E7_RICCO (Mitochondrial oxoglutarate/malate carrier protein, putative OS=Ricinus communis GN=RCOM_1361330 PE=3 SV=1)

HSP 1 Score: 545.4 bits (1404), Expect = 4.2e-152
Identity = 267/300 (89.00%), Postives = 284/300 (94.67%), Query Frame = 1

Query: 1   MADDKKPSVSP-VWPTVKPFVNGGASGMLATCVIQPIDMVKVRIQLGQGSAGHVTRTMLK 60
           M D+KK   +  VWPTVKPFVNGGASGMLATCVIQPIDM+KVRIQLGQGSA  VTRTMLK
Sbjct: 1   MGDEKKSQAAAGVWPTVKPFVNGGASGMLATCVIQPIDMIKVRIQLGQGSAAQVTRTMLK 60

Query: 61  EEGFGAFYKGLSAGLLRQATYTTARLGSFKILTNKAIEANEGKPLPLYQKALCGLTAGAI 120
           EEGFGAFYKGLSAGLLRQATYTTARLGSFKILTNKAIEAN+GKPLPLYQKALCGLTAGAI
Sbjct: 61  EEGFGAFYKGLSAGLLRQATYTTARLGSFKILTNKAIEANDGKPLPLYQKALCGLTAGAI 120

Query: 121 GASVGSPADLALIRMQADATLPAAQRRNYKNAFHALYRILADEGVLALWKGAGPTIVRAM 180
           GA+VGSPADLALIRMQADATLPAAQRRNYKNAFHALYRI +DEGVLALWKGAGPT+VRAM
Sbjct: 121 GATVGSPADLALIRMQADATLPAAQRRNYKNAFHALYRISSDEGVLALWKGAGPTVVRAM 180

Query: 181 GLNMGMLASYDQSVEFFKDNLGFGEAATVLGASMVSGFFASACSLPFDYVKTQIQKMQPD 240
            LNMGMLASYDQ+VEFF+D++G  EAATV+GAS VSGFFASACSLPFDYVKTQIQKMQPD
Sbjct: 181 ALNMGMLASYDQTVEFFRDSVGLSEAATVIGASAVSGFFASACSLPFDYVKTQIQKMQPD 240

Query: 241 AEGKFPYSGSLDCAMKTLKAGGPLKFYTGFPVYCVRIAPHVMMTWIFLNQIQKVEKSYGL 300
           A+GK+PY+GSLDCAMKTLK+GGP KFYTGFPVYCVRIAPHVMMTWIFLNQIQK EK+ GL
Sbjct: 241 AQGKYPYTGSLDCAMKTLKSGGPFKFYTGFPVYCVRIAPHVMMTWIFLNQIQKFEKTVGL 300

BLAST of CSPI04G12550.1 vs. TAIR10
Match: AT5G19760.1 (AT5G19760.1 Mitochondrial substrate carrier family protein)

HSP 1 Score: 508.4 bits (1308), Expect = 2.9e-144
Identity = 249/299 (83.28%), Postives = 270/299 (90.30%), Query Frame = 1

Query: 1   MADDKKPSVSPVWPTVKPFVNGGASGMLATCVIQPIDMVKVRIQLGQGSAGHVTRTMLKE 60
           MA++KK  +S VW TVKPFVNGGASGMLATCVIQPIDM+KVRIQLGQGSA  +T  MLK 
Sbjct: 1   MAEEKKAPIS-VWTTVKPFVNGGASGMLATCVIQPIDMIKVRIQLGQGSAASITTNMLKN 60

Query: 61  EGFGAFYKGLSAGLLRQATYTTARLGSFKILTNKAIEANEGKPLPLYQKALCGLTAGAIG 120
           EG GAFYKGLSAGLLRQATYTTARLGSFK+LT KAIE+N+GKPLPLYQKALCGLTAGAIG
Sbjct: 61  EGVGAFYKGLSAGLLRQATYTTARLGSFKLLTAKAIESNDGKPLPLYQKALCGLTAGAIG 120

Query: 121 ASVGSPADLALIRMQADATLPAAQRRNYKNAFHALYRILADEGVLALWKGAGPTIVRAMG 180
           A VGSPADLALIRMQAD TLP AQRRNY NAFHAL RI ADEGVLALWKG GPT+VRAM 
Sbjct: 121 ACVGSPADLALIRMQADNTLPLAQRRNYTNAFHALTRISADEGVLALWKGCGPTVVRAMA 180

Query: 181 LNMGMLASYDQSVEFFKDNLGFGEAATVLGASMVSGFFASACSLPFDYVKTQIQKMQPDA 240
           LNMGMLASYDQS E+ +DNLGFGE +TV+GAS VSGF A+ACSLPFD+VKTQIQKMQPDA
Sbjct: 181 LNMGMLASYDQSAEYMRDNLGFGEMSTVVGASAVSGFCAAACSLPFDFVKTQIQKMQPDA 240

Query: 241 EGKFPYSGSLDCAMKTLKAGGPLKFYTGFPVYCVRIAPHVMMTWIFLNQIQKVEKSYGL 300
           +GK+PY+GSLDCAMKTLK GGPLKFY+GFPVYCVRIAPHVMMTWIFLNQI K +K  G+
Sbjct: 241 QGKYPYTGSLDCAMKTLKEGGPLKFYSGFPVYCVRIAPHVMMTWIFLNQITKFQKKIGM 298

BLAST of CSPI04G12550.1 vs. TAIR10
Match: AT2G22500.1 (AT2G22500.1 uncoupling protein 5)

HSP 1 Score: 186.4 bits (472), Expect = 2.5e-47
Identity = 103/247 (41.70%), Postives = 149/247 (60.32%), Query Frame = 1

Query: 57  MLKEEGFGAFYKGLSAGLLRQATYTTARLGSFKILTNKAIEANEGKPLPLYQKALCGLTA 116
           +++EEG  A + G+SA +LRQ  Y+T R+G + I+  +  +  E K +PL +K   G  A
Sbjct: 71  LIREEGMRALFSGVSATVLRQTLYSTTRMGLYDIIKGEWTDP-ETKTMPLMKKIGAGAIA 130

Query: 117 GAIGASVGSPADLALIRMQADATLPAAQRRNYKNAFHALYRILADEGVLALWKGAGPTIV 176
           GAIGA+VG+PAD+A++RMQAD  LP   RRNYK+   A+ +++  EGV +LW+G+  TI 
Sbjct: 131 GAIGAAVGNPADVAMVRMQADGRLPLTDRRNYKSVLDAITQMIRGEGVTSLWRGSSLTIN 190

Query: 177 RAMGLNMGMLASYDQSVE------FFKDNLGFGEAATVLGASMVSGFFASACSLPFDYVK 236
           RAM +    LASYD   E        KD LG     T + AS  +GF AS  S P D +K
Sbjct: 191 RAMLVTSSQLASYDSVKETILEKGLLKDGLG-----THVSASFAAGFVASVASNPVDVIK 250

Query: 237 TQIQKMQPDAEGKFPYSGSLDCAMKTLKAGGPLKFYTGFPVYCVRIAPHVMMTWIFLNQI 296
           T++  M+  A    PY G++DCA+KT+KA G +  Y GF     R AP  ++ ++ L Q+
Sbjct: 251 TRVMNMKVVAGVAPPYKGAVDCALKTVKAEGIMSLYKGFIPTVSRQAPFTVVLFVTLEQV 310

Query: 297 QKVEKSY 298
           +K+ K Y
Sbjct: 311 KKLFKDY 311

BLAST of CSPI04G12550.1 vs. TAIR10
Match: AT4G24570.1 (AT4G24570.1 dicarboxylate carrier 2)

HSP 1 Score: 179.5 bits (454), Expect = 3.1e-45
Identity = 101/242 (41.74%), Postives = 145/242 (59.92%), Query Frame = 1

Query: 57  MLKEEGFGAFYKGLSAGLLRQATYTTARLGSFKILTNKAIEANEGKPLPLYQKALCGLTA 116
           ++K EG  A + G+SA LLRQ  Y+T R+G +++L NK  +   GK L L +K   GL A
Sbjct: 78  IVKSEGAAALFSGVSATLLRQTLYSTTRMGLYEVLKNKWTDPESGK-LNLSRKIGAGLVA 137

Query: 117 GAIGASVGSPADLALIRMQADATLPAAQRRNYKNAFHALYRILADEGVLALWKGAGPTIV 176
           G IGA+VG+PAD+A++RMQAD  LP AQRRNY     A+  ++  EGV +LW+G+  TI 
Sbjct: 138 GGIGAAVGNPADVAMVRMQADGRLPLAQRRNYAGVGDAIRSMVKGEGVTSLWRGSALTIN 197

Query: 177 RAMGLNMGMLASYDQSVEFFKDNLGFGEA-ATVLGASMVSGFFASACSLPFDYVKTQIQK 236
           RAM +    LASYDQ  E   +N    +   T + AS  +GF AS  S P D +KT++  
Sbjct: 198 RAMIVTAAQLASYDQFKEGILENGVMNDGLGTHVVASFAAGFVASVASNPVDVIKTRVMN 257

Query: 237 MQPDAEGKFPYSGSLDCAMKTLKAGGPLKFYTGFPVYCVRIAPHVMMTWIFLNQIQKVEK 296
           M+  A     Y G+ DCA+KT+KA G +  Y GF     R  P  ++ ++ L Q++K+ +
Sbjct: 258 MKVGA-----YDGAWDCAVKTVKAEGAMALYKGFVPTVCRQGPFTVVLFVTLEQVRKLLR 313

Query: 297 SY 298
            +
Sbjct: 318 DF 313

BLAST of CSPI04G12550.1 vs. TAIR10
Match: AT5G09470.1 (AT5G09470.1 dicarboxylate carrier 3)

HSP 1 Score: 152.9 bits (385), Expect = 3.1e-37
Identity = 93/241 (38.59%), Postives = 133/241 (55.19%), Query Frame = 1

Query: 57  MLKEEGFGAFYKGLSAGLLRQATYTTARLGSFKILTNKAIEANEGKPLPLYQKALCGLTA 116
           ++K EG  A + G+SA +LRQ  Y+  R+G +  L  +  +   G   PL  K   GL A
Sbjct: 99  IVKTEGPAALFSGVSATILRQMLYSATRMGIYDFLKRRWTDQLTGN-FPLVTKITAGLIA 158

Query: 117 GAIGASVGSPADLALIRMQADATLPAAQRRNYKNAFHALYRILADEGVLALWKGAGPTIV 176
           GA+G+ VG+PAD+A++RMQAD +LP  +RRNYK+   A+ RI   EGV +LW+G+  T+ 
Sbjct: 159 GAVGSVVGNPADVAMVRMQADGSLPLNRRRNYKSVVDAIDRIARQEGVSSLWRGSWLTVN 218

Query: 177 RAMGLNMGMLASYDQSVEFF-KDNLGF-GEAATVLGASMVSGFFASACSLPFDYVKTQIQ 236
           RAM +    LA+YD   E       G  G   T + AS  +G  A+  S P D VKT++ 
Sbjct: 219 RAMIVTASQLATYDHVKEILVAGGRGTPGGIGTHVAASFAAGIVAAVASNPIDVVKTRM- 278

Query: 237 KMQPDAEGKFPYSGSLDCAMKTLKAGGPLKFYTGFPVYCVRIAPHVMMTWIFLNQIQKVE 296
            M  D E    Y G LDCA+K +   GP+  Y G      R  P  M+ ++ L Q++ + 
Sbjct: 279 -MNADKE---IYGGPLDCAVKMVAEEGPMALYKGLVPTATRQGPFTMILFLTLEQVRGLL 333

BLAST of CSPI04G12550.1 vs. TAIR10
Match: AT3G54110.1 (AT3G54110.1 plant uncoupling mitochondrial protein 1)

HSP 1 Score: 152.5 bits (384), Expect = 4.1e-37
Identity = 101/286 (35.31%), Postives = 148/286 (51.75%), Query Frame = 1

Query: 24  ASGMLATCVIQ----PIDMVKVRIQLGQGS-AGHVTR-----------TMLKEEGFGAFY 83
           A    A CV +    P+D  KVR+QL + + AG VT            T+ +EEG  + +
Sbjct: 16  ACSAFAACVGEVCTIPLDTAKVRLQLQKSALAGDVTLPKYRGLLGTVGTIAREEGLRSLW 75

Query: 84  KGLSAGLLRQATYTTARLGSFKILTNKAIEANEGKPLPLYQKALCGLTAGAIGASVGSPA 143
           KG+  GL RQ  +   R+G ++ + N  +  +    +PL +K L GLT GA+G  V +P 
Sbjct: 76  KGVVPGLHRQCLFGGLRIGMYEPVKNLYVGKDFVGDVPLSKKILAGLTTGALGIMVANPT 135

Query: 144 DLALIRMQADATLPAAQRRNYKNAFHALYRILADEGVLALWKGAGPTIVRAMGLNMGMLA 203
           DL  +R+QA+  L A   R Y  A +A   I+  EGV ALW G GP + R   +N   LA
Sbjct: 136 DLVKVRLQAEGKLAAGAPRRYSGALNAYSTIVRQEGVRALWTGLGPNVARNAIINAAELA 195

Query: 204 SYDQSVEFFKDNLGFGE-AATVLGASMVSGFFASACSLPFDYVKTQIQKMQPDAEGKFPY 263
           SYDQ  E      GF +   T + + + +GFFA     P D VK+   +M  D+     Y
Sbjct: 196 SYDQVKETILKIPGFTDNVVTHILSGLGAGFFAVCIGSPVDVVKS---RMMGDSGA---Y 255

Query: 264 SGSLDCAMKTLKAGGPLKFYTGFPVYCVRIAPHVMMTWIFLNQIQK 293
            G++DC +KTLK+ GP+ FY GF     R+    ++ ++ L Q +K
Sbjct: 256 KGTIDCFVKTLKSDGPMAFYKGFIPNFGRLGSWNVIMFLTLEQAKK 295

BLAST of CSPI04G12550.1 vs. NCBI nr
Match: gi|449463851|ref|XP_004149644.1| (PREDICTED: mitochondrial dicarboxylate/tricarboxylate transporter DTC [Cucumis sativus])

HSP 1 Score: 601.3 bits (1549), Expect = 9.4e-169
Identity = 298/299 (99.67%), Postives = 299/299 (100.00%), Query Frame = 1

Query: 1   MADDKKPSVSPVWPTVKPFVNGGASGMLATCVIQPIDMVKVRIQLGQGSAGHVTRTMLKE 60
           MADDKKPSVSPVWPTVKPFVNGGASGMLATCVIQPIDMVKVRIQLGQGSAGHVTRTMLKE
Sbjct: 1   MADDKKPSVSPVWPTVKPFVNGGASGMLATCVIQPIDMVKVRIQLGQGSAGHVTRTMLKE 60

Query: 61  EGFGAFYKGLSAGLLRQATYTTARLGSFKILTNKAIEANEGKPLPLYQKALCGLTAGAIG 120
           EGFGAFYKGLSAGLLRQATYTTARLGSFKILTNKAIEANEGKPLPLYQKALCGLTAGAIG
Sbjct: 61  EGFGAFYKGLSAGLLRQATYTTARLGSFKILTNKAIEANEGKPLPLYQKALCGLTAGAIG 120

Query: 121 ASVGSPADLALIRMQADATLPAAQRRNYKNAFHALYRILADEGVLALWKGAGPTIVRAMG 180
           ASVGSPADLALIRMQADATLPAAQRRNYKNAFHALYRILADEGVLALWKGAGPTIVRAMG
Sbjct: 121 ASVGSPADLALIRMQADATLPAAQRRNYKNAFHALYRILADEGVLALWKGAGPTIVRAMG 180

Query: 181 LNMGMLASYDQSVEFFKDNLGFGEAATVLGASMVSGFFASACSLPFDYVKTQIQKMQPDA 240
           LNMGMLASYDQSVEFFKDNLGFGEAATVLGASMVSGFFASACSLPFDYVKTQIQKMQPDA
Sbjct: 181 LNMGMLASYDQSVEFFKDNLGFGEAATVLGASMVSGFFASACSLPFDYVKTQIQKMQPDA 240

Query: 241 EGKFPYSGSLDCAMKTLKAGGPLKFYTGFPVYCVRIAPHVMMTWIFLNQIQKVEKSYGL 300
           EGKFP+SGSLDCAMKTLKAGGPLKFYTGFPVYCVRIAPHVMMTWIFLNQIQKVEKSYGL
Sbjct: 241 EGKFPHSGSLDCAMKTLKAGGPLKFYTGFPVYCVRIAPHVMMTWIFLNQIQKVEKSYGL 299

BLAST of CSPI04G12550.1 vs. NCBI nr
Match: gi|659097931|ref|XP_008449887.1| (PREDICTED: mitochondrial dicarboxylate/tricarboxylate transporter DTC isoform X1 [Cucumis melo])

HSP 1 Score: 589.0 bits (1517), Expect = 4.8e-165
Identity = 292/299 (97.66%), Postives = 295/299 (98.66%), Query Frame = 1

Query: 1   MADDKKPSVSPVWPTVKPFVNGGASGMLATCVIQPIDMVKVRIQLGQGSAGHVTRTMLKE 60
           MADDKKP+ S VWPTVKPFVNGGASGMLATCVIQPIDMVKVRIQLGQGSAG VTRTMLKE
Sbjct: 1   MADDKKPTFSSVWPTVKPFVNGGASGMLATCVIQPIDMVKVRIQLGQGSAGQVTRTMLKE 60

Query: 61  EGFGAFYKGLSAGLLRQATYTTARLGSFKILTNKAIEANEGKPLPLYQKALCGLTAGAIG 120
           EGFGAFYKGLSAGLLRQATYTTARLGSFK+LTNKAIEANEGKPLPLYQKALCGLTAGAIG
Sbjct: 61  EGFGAFYKGLSAGLLRQATYTTARLGSFKMLTNKAIEANEGKPLPLYQKALCGLTAGAIG 120

Query: 121 ASVGSPADLALIRMQADATLPAAQRRNYKNAFHALYRILADEGVLALWKGAGPTIVRAMG 180
           ASVGSPADLALIRMQADATLPAAQRRNYKNAFHALYRILADEGVLALWKGAGPTIVRAMG
Sbjct: 121 ASVGSPADLALIRMQADATLPAAQRRNYKNAFHALYRILADEGVLALWKGAGPTIVRAMG 180

Query: 181 LNMGMLASYDQSVEFFKDNLGFGEAATVLGASMVSGFFASACSLPFDYVKTQIQKMQPDA 240
           LNMGMLASYDQSVEFFKDNLGFGEAATVLGAS VSGFFASACSLPFDYVKTQIQKMQPDA
Sbjct: 181 LNMGMLASYDQSVEFFKDNLGFGEAATVLGASTVSGFFASACSLPFDYVKTQIQKMQPDA 240

Query: 241 EGKFPYSGSLDCAMKTLKAGGPLKFYTGFPVYCVRIAPHVMMTWIFLNQIQKVEKSYGL 300
           EGKFPYSGSLDCAMKTLKAGGPLKFYTGFPVYCVRIAPHVMMTWIFLNQIQKVEKS+GL
Sbjct: 241 EGKFPYSGSLDCAMKTLKAGGPLKFYTGFPVYCVRIAPHVMMTWIFLNQIQKVEKSFGL 299

BLAST of CSPI04G12550.1 vs. NCBI nr
Match: gi|802563789|ref|XP_012067077.1| (PREDICTED: mitochondrial dicarboxylate/tricarboxylate transporter DTC [Jatropha curcas])

HSP 1 Score: 553.9 bits (1426), Expect = 1.7e-154
Identity = 269/299 (89.97%), Postives = 286/299 (95.65%), Query Frame = 1

Query: 1   MADDKKPSVSPVWPTVKPFVNGGASGMLATCVIQPIDMVKVRIQLGQGSAGHVTRTMLKE 60
           M ++KK   + VWPTVKPFVNGGASGMLATCVIQPIDM+KVRIQLGQGSA  VT+TMLKE
Sbjct: 1   MGEEKKAQPAGVWPTVKPFVNGGASGMLATCVIQPIDMIKVRIQLGQGSAAQVTKTMLKE 60

Query: 61  EGFGAFYKGLSAGLLRQATYTTARLGSFKILTNKAIEANEGKPLPLYQKALCGLTAGAIG 120
           EGFGAFYKGLSAGLLRQATYTTARLGSF++LTNKAIEAN+GKPLPLYQKALCGLTAGAIG
Sbjct: 61  EGFGAFYKGLSAGLLRQATYTTARLGSFRMLTNKAIEANDGKPLPLYQKALCGLTAGAIG 120

Query: 121 ASVGSPADLALIRMQADATLPAAQRRNYKNAFHALYRILADEGVLALWKGAGPTIVRAMG 180
           A VGSPADLALIRMQADATLPAAQRRNY NAFHALYRI+ADEGVLALWKGAGPT+VRAM 
Sbjct: 121 ACVGSPADLALIRMQADATLPAAQRRNYTNAFHALYRIVADEGVLALWKGAGPTVVRAMA 180

Query: 181 LNMGMLASYDQSVEFFKDNLGFGEAATVLGASMVSGFFASACSLPFDYVKTQIQKMQPDA 240
           LNMGMLASYDQSVEFF+DNLGFGEAATV+GAS VSGFFASACSLPFDYVKTQIQKMQPDA
Sbjct: 181 LNMGMLASYDQSVEFFRDNLGFGEAATVIGASSVSGFFASACSLPFDYVKTQIQKMQPDA 240

Query: 241 EGKFPYSGSLDCAMKTLKAGGPLKFYTGFPVYCVRIAPHVMMTWIFLNQIQKVEKSYGL 300
           +GK+PY+GSLDCAMKTLKAGGP KFYTGFPVYCVRIAPHVMMTWIFLNQIQK+EK+ GL
Sbjct: 241 QGKYPYTGSLDCAMKTLKAGGPFKFYTGFPVYCVRIAPHVMMTWIFLNQIQKLEKNVGL 299

BLAST of CSPI04G12550.1 vs. NCBI nr
Match: gi|703160837|ref|XP_010112635.1| (hypothetical protein L484_019087 [Morus notabilis])

HSP 1 Score: 552.7 bits (1423), Expect = 3.8e-154
Identity = 268/299 (89.63%), Postives = 285/299 (95.32%), Query Frame = 1

Query: 1   MADDKKPSVSPVWPTVKPFVNGGASGMLATCVIQPIDMVKVRIQLGQGSAGHVTRTMLKE 60
           M D+KKP  + VWPTVKPFVNGGASGMLATCVIQPIDM+KVRIQLGQGSAG VT+TMLKE
Sbjct: 1   MGDEKKPKSTGVWPTVKPFVNGGASGMLATCVIQPIDMIKVRIQLGQGSAGQVTKTMLKE 60

Query: 61  EGFGAFYKGLSAGLLRQATYTTARLGSFKILTNKAIEANEGKPLPLYQKALCGLTAGAIG 120
           EG GAFYKGLSAGLLRQATYTTARLGSFKILTNKAIEAN+GKPLPLYQKALCGLTAGAIG
Sbjct: 61  EGVGAFYKGLSAGLLRQATYTTARLGSFKILTNKAIEANDGKPLPLYQKALCGLTAGAIG 120

Query: 121 ASVGSPADLALIRMQADATLPAAQRRNYKNAFHALYRILADEGVLALWKGAGPTIVRAMG 180
           ASVGSPADLALIRMQADATLP AQRRNYKNAFHALYRI++DEGVLALWKGAGPT+VRAM 
Sbjct: 121 ASVGSPADLALIRMQADATLPVAQRRNYKNAFHALYRIVSDEGVLALWKGAGPTVVRAMA 180

Query: 181 LNMGMLASYDQSVEFFKDNLGFGEAATVLGASMVSGFFASACSLPFDYVKTQIQKMQPDA 240
           LNMGMLASYDQSVEFFKD++G GEA+TVL AS VSGFFA+ACSLPFDYVKTQIQKMQPDA
Sbjct: 181 LNMGMLASYDQSVEFFKDSVGLGEASTVLAASSVSGFFAAACSLPFDYVKTQIQKMQPDA 240

Query: 241 EGKFPYSGSLDCAMKTLKAGGPLKFYTGFPVYCVRIAPHVMMTWIFLNQIQKVEKSYGL 300
           +GK+PY+GSLDCA+KTLKAGGP KFYTGFPVYCVRIAPHVMMTWIFLNQ+QK EKS GL
Sbjct: 241 QGKYPYTGSLDCALKTLKAGGPFKFYTGFPVYCVRIAPHVMMTWIFLNQVQKFEKSIGL 299

BLAST of CSPI04G12550.1 vs. NCBI nr
Match: gi|702278692|ref|XP_010044939.1| (PREDICTED: mitochondrial dicarboxylate/tricarboxylate transporter DTC [Eucalyptus grandis])

HSP 1 Score: 551.6 bits (1420), Expect = 8.5e-154
Identity = 270/299 (90.30%), Postives = 283/299 (94.65%), Query Frame = 1

Query: 1   MADDKKPSVSPVWPTVKPFVNGGASGMLATCVIQPIDMVKVRIQLGQGSAGHVTRTMLKE 60
           M ++KKP  + VWPTVKPFVNGGASGMLATCVIQPIDM+KVRIQLGQGSAG VTR MLKE
Sbjct: 1   MGEEKKPKPAGVWPTVKPFVNGGASGMLATCVIQPIDMIKVRIQLGQGSAGQVTRNMLKE 60

Query: 61  EGFGAFYKGLSAGLLRQATYTTARLGSFKILTNKAIEANEGKPLPLYQKALCGLTAGAIG 120
           EG GAFYKGLSAGLLRQATYTTARLGSF+ILT KAIEAN+GKPLPLYQKALCGLTAGAIG
Sbjct: 61  EGMGAFYKGLSAGLLRQATYTTARLGSFRILTAKAIEANDGKPLPLYQKALCGLTAGAIG 120

Query: 121 ASVGSPADLALIRMQADATLPAAQRRNYKNAFHALYRILADEGVLALWKGAGPTIVRAMG 180
           ASVGSPADLALIRMQADATLPAAQRR+Y NAFHALYRI+ DEGVLALWKGAGPT+VRAM 
Sbjct: 121 ASVGSPADLALIRMQADATLPAAQRRHYTNAFHALYRIVGDEGVLALWKGAGPTVVRAMA 180

Query: 181 LNMGMLASYDQSVEFFKDNLGFGEAATVLGASMVSGFFASACSLPFDYVKTQIQKMQPDA 240
           LNMGMLASYDQ VEFFKD+LGFGE ATVLGAS VSGFFA+ACSLPFDYVKTQIQKMQPDA
Sbjct: 181 LNMGMLASYDQGVEFFKDSLGFGEGATVLGASTVSGFFAAACSLPFDYVKTQIQKMQPDA 240

Query: 241 EGKFPYSGSLDCAMKTLKAGGPLKFYTGFPVYCVRIAPHVMMTWIFLNQIQKVEKSYGL 300
           EGK+PY+GSLDCAMKTLKAGGPLKFYTGFPVYCVRIAPHVMMTWIFLNQIQK EKS GL
Sbjct: 241 EGKYPYTGSLDCAMKTLKAGGPLKFYTGFPVYCVRIAPHVMMTWIFLNQIQKFEKSVGL 299

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
DTC_ARATH5.2e-14383.28Mitochondrial dicarboxylate/tricarboxylate transporter DTC OS=Arabidopsis thalia... [more]
M2OM_NEUCR3.5e-6748.94Putative mitochondrial 2-oxoglutarate/malate carrier protein OS=Neurospora crass... [more]
M2OM_MOUSE2.9e-6146.84Mitochondrial 2-oxoglutarate/malate carrier protein OS=Mus musculus GN=Slc25a11 ... [more]
M2OM_HUMAN5.0e-6146.51Mitochondrial 2-oxoglutarate/malate carrier protein OS=Homo sapiens GN=SLC25A11 ... [more]
M2OM_BOVIN1.9e-6045.85Mitochondrial 2-oxoglutarate/malate carrier protein OS=Bos taurus GN=SLC25A11 PE... [more]
Match NameE-valueIdentityDescription
A0A0A0KZ03_CUCSA6.5e-16999.67Uncharacterized protein OS=Cucumis sativus GN=Csa_4G268080 PE=3 SV=1[more]
A0A067LC67_JATCU1.2e-15489.97Uncharacterized protein OS=Jatropha curcas GN=JCGZ_01873 PE=3 SV=1[more]
W9SZF7_9ROSA2.7e-15489.63Uncharacterized protein OS=Morus notabilis GN=L484_019087 PE=3 SV=1[more]
A0A059D9A4_EUCGR5.9e-15490.30Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_B03603 PE=3 SV=1[more]
B9T1E7_RICCO4.2e-15289.00Mitochondrial oxoglutarate/malate carrier protein, putative OS=Ricinus communis ... [more]
Match NameE-valueIdentityDescription
AT5G19760.12.9e-14483.28 Mitochondrial substrate carrier family protein[more]
AT2G22500.12.5e-4741.70 uncoupling protein 5[more]
AT4G24570.13.1e-4541.74 dicarboxylate carrier 2[more]
AT5G09470.13.1e-3738.59 dicarboxylate carrier 3[more]
AT3G54110.14.1e-3735.31 plant uncoupling mitochondrial protein 1[more]
Match NameE-valueIdentityDescription
gi|449463851|ref|XP_004149644.1|9.4e-16999.67PREDICTED: mitochondrial dicarboxylate/tricarboxylate transporter DTC [Cucumis s... [more]
gi|659097931|ref|XP_008449887.1|4.8e-16597.66PREDICTED: mitochondrial dicarboxylate/tricarboxylate transporter DTC isoform X1... [more]
gi|802563789|ref|XP_012067077.1|1.7e-15489.97PREDICTED: mitochondrial dicarboxylate/tricarboxylate transporter DTC [Jatropha ... [more]
gi|703160837|ref|XP_010112635.1|3.8e-15489.63hypothetical protein L484_019087 [Morus notabilis][more]
gi|702278692|ref|XP_010044939.1|8.5e-15490.30PREDICTED: mitochondrial dicarboxylate/tricarboxylate transporter DTC [Eucalyptu... [more]
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
IPR018108Mitochondrial_sb/sol_carrier
IPR023395Mt_carrier_dom_sf
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006835 dicarboxylic acid transport
biological_process GO:0035674 tricarboxylic acid transmembrane transport
cellular_component GO:0005618 cell wall
cellular_component GO:0009941 chloroplast envelope
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005739 mitochondrion
cellular_component GO:0009506 plasmodesma
cellular_component GO:0005774 vacuolar membrane
molecular_function GO:0005310 dicarboxylic acid transmembrane transporter activity
molecular_function GO:0015142 tricarboxylic acid transmembrane transporter activity

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CSPI04G12550CSPI04G12550gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CSPI04G12550.1CSPI04G12550.1-proteinpolypeptide


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CSPI04G12550.1.utr3p1CSPI04G12550.1.utr3p1three_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CSPI04G12550.1.cds6CSPI04G12550.1.cds6CDS
CSPI04G12550.1.cds5CSPI04G12550.1.cds5CDS
CSPI04G12550.1.cds4CSPI04G12550.1.cds4CDS
CSPI04G12550.1.cds3CSPI04G12550.1.cds3CDS
CSPI04G12550.1.cds2CSPI04G12550.1.cds2CDS
CSPI04G12550.1.cds1CSPI04G12550.1.cds1CDS


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CSPI04G12550.1.utr5p1CSPI04G12550.1.utr5p1five_prime_UTR


Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR018108Mitochondrial substrate/solute carrierPFAMPF00153Mito_carrcoord: 103..199
score: 4.1E-19coord: 209..293
score: 2.5E-12coord: 15..91
score: 8.3
IPR018108Mitochondrial substrate/solute carrierPROFILEPS50920SOLCARcoord: 13..94
score: 20.132coord: 104..195
score: 23.629coord: 203..293
score: 17
IPR023395Mitochondrial carrier domainGENE3DG3DSA:1.50.40.10coord: 15..293
score: 1.0
IPR023395Mitochondrial carrier domainunknownSSF103506Mitochondrial carriercoord: 12..287
score: 1.02
NoneNo IPR availablePANTHERPTHR24089FAMILY NOT NAMEDcoord: 1..293
score: 3.6E
NoneNo IPR availablePANTHERPTHR24089:SF266MITOCHONDRIAL DICARBOXYLATE/TRICARBOXYLATE TRANSPORTER DTCcoord: 1..293
score: 3.6E