CSPI04G08560.1 (mRNA) Wild cucumber (PI 183967)

NameCSPI04G08560.1
TypemRNA
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionAlpha/beta-Hydrolases superfamily protein
LocationChr4 : 6328610 .. 6329604 (-)
Sequence length894
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCAAACCACCATTCTCGTCTAATCTATAGCAAGAAACTCAAAAGAATGGCCGCTATTTCTATCGATCCAAGGCTCAAACTCCAAATACCAAAGCTCCGTATTCAAAATGAAGCCACAACAGAAGAGATTGAAGGGGTAATTCGAGTTTATGCAGATGGGCATGTTGAAAGGCCCCCAATCATCCCAAATGTATCCTGCAGCATCACAGCATCAGACAAACGCGTAATTGCAAAAGATGTTCTCATAGAAAATTTAACAAACACCTGGGCGCGAATTTACGTAGCTACCTCTCAACGAAACCTCCCCTTACTTGTTTATTTCCATGGCGGTGGCTTCTGTGTTGGCTCTACCGCATGGAGCTGTTACCATGAATTCCTGCTCGACCTTGCTTCCAAAGCAAAATGTGTAATACTCTCAGTTAACTATCGTCTTTCCCCTGAAAATCGCCTCCCCGCCGCTTACGAAGACGGATTCAACACACTCATGTGGTTGAAACAACAAATAATAACCAACCCAATTGAACACAAATGGTGATTAGGCAATTCAAATGTATCAAAACTATTCCTCGTCGGCGATAGCGCCGGAGCAAACATAGCATACAACATCACCACACGACTAAGGCTCAACGAACCCACAATCCTAAAACCCCTAACCCTAAAGGGCATAATCTTGATTCAACCTTTCTTTGGCGGAGAATCATGAACCAAATCAGAAAAGCTCACCGCTCAACCATCTTTCGGTTTTGCATTAACCCTCGATGCCGCCGACACATACTGGCGTCTGTCCCTGCCAGCGGGGGCCAATCGCGACCATACATGGAGCAATCCGCTGGTGAATGGCGTGGCAAAGTTGAGAGAAACAAAGGTGCCCCCGTTAATGGTGTGCATATCGGAGTTGGACATACTGGTGGACAGAAACTTGGAGTTCTGTGCAGCGATGGCGGCGGCCAAGAAGAAAGTGGAGAGAGTAATATTGAAAGGAGTTGGACATAG

mRNA sequence

ATGCCAAACCACCATTCTCGTCTAATCTATAGCAAGAAACTCAAAAGAATGGCCGCTATTTCTATCGATCCAAGGCTCAAACTCCAAATACCAAAGCTCCGTATTCAAAATGAAGCCACAACAGAAGAGATTGAAGGGGTAATTCGAGTTTATGCAGATGGGCATGTTGAAAGGCCCCCAATCATCCCAAATGTATCCTGCAGCATCACAGCATCAGACAAACGCGTAATTGCAAAAGATGTTCTCATAGAAAATTTAACAAACACCTGGGCGCGAATTTACGTAGCTACCTCTCAACGAAACCTCCCCTTACTTGTTTATTTCCATGGCGGTGGCTTCTGTGTTGGCTCTACCGCATGGAGCTGTTACCATGAATTCCTGCTCGACCTTGCTTCCAAAGCAAAATGTGTAATACTCTCAGTTAACTATCGTCTTTCCCCTGAAAATCGCCTCCCCGCCGCTTACGAAGACGGATTCAACACACTCATCAAACATAGCATACAACATCACCACACGACTAAGGCTCAACGAACCCACAATCCTAAAACCCCTAACCCTAAAGGGCATAATCTTGATTCAACCTTTCTTTGGCGGAGAATCATGAACCAAATCAGAAAAGCTCACCGCTCAACCATCTTTCGGTTTTGCATTAACCCTCGATGCCGCCGACACATACTGGCGTCTGTCCCTGCCAGCGGGGGCCAATCGCGACCATACATGGAGCAATCCGCTGGTGAATGGCGTGGCAAAGTTGAGAGAAACAAAGGTGCCCCCGTTAATGGTGTGCATATCGGAGTTGGACATACTGGTGGACAGAAACTTGGAGTTCTGTGCAGCGATGGCGGCGGCCAAGAAGAAAGTGGAGAGAGTAATATTGAAAGGAGTTGGACATAG

Coding sequence (CDS)

ATGCCAAACCACCATTCTCGTCTAATCTATAGCAAGAAACTCAAAAGAATGGCCGCTATTTCTATCGATCCAAGGCTCAAACTCCAAATACCAAAGCTCCGTATTCAAAATGAAGCCACAACAGAAGAGATTGAAGGGGTAATTCGAGTTTATGCAGATGGGCATGTTGAAAGGCCCCCAATCATCCCAAATGTATCCTGCAGCATCACAGCATCAGACAAACGCGTAATTGCAAAAGATGTTCTCATAGAAAATTTAACAAACACCTGGGCGCGAATTTACGTAGCTACCTCTCAACGAAACCTCCCCTTACTTGTTTATTTCCATGGCGGTGGCTTCTGTGTTGGCTCTACCGCATGGAGCTGTTACCATGAATTCCTGCTCGACCTTGCTTCCAAAGCAAAATGTGTAATACTCTCAGTTAACTATCGTCTTTCCCCTGAAAATCGCCTCCCCGCCGCTTACGAAGACGGATTCAACACACTCATCAAACATAGCATACAACATCACCACACGACTAAGGCTCAACGAACCCACAATCCTAAAACCCCTAACCCTAAAGGGCATAATCTTGATTCAACCTTTCTTTGGCGGAGAATCATGAACCAAATCAGAAAAGCTCACCGCTCAACCATCTTTCGGTTTTGCATTAACCCTCGATGCCGCCGACACATACTGGCGTCTGTCCCTGCCAGCGGGGGCCAATCGCGACCATACATGGAGCAATCCGCTGGTGAATGGCGTGGCAAAGTTGAGAGAAACAAAGGTGCCCCCGTTAATGGTGTGCATATCGGAGTTGGACATACTGGTGGACAGAAACTTGGAGTTCTGTGCAGCGATGGCGGCGGCCAAGAAGAAAGTGGAGAGAGTAATATTGAAAGGAGTTGGACATAG
BLAST of CSPI04G08560.1 vs. Swiss-Prot
Match: CXE17_ARATH (Probable carboxylesterase 17 OS=Arabidopsis thaliana GN=CXE17 PE=2 SV=1)

HSP 1 Score: 156.0 bits (393), Expect = 6.5e-37
Identity = 80/134 (59.70%), Postives = 98/134 (73.13%), Query Frame = 1

Query: 42  EEIEGVIRVYADGHVERPPIIPNVSCSITASDKRVIAKDVLIENLTNTWARIYV-----A 101
           EEIEG+I+V+ DG VERPPI+P VS +I  S K   A D+ + N  +TW R+Y+     A
Sbjct: 30  EEIEGLIKVFNDGCVERPPIVPIVSPTIHPSSKAT-AFDIKLSN--DTWTRVYIPDAAAA 89

Query: 102 TSQRNLPLLVYFHGGGFCVGSTAWSCYHEFLLDLASKAKCVILSVNYRLSPENRLPAAYE 161
           +    LPLLVYFHGGGFCVGS AWSCYH+FL  LA KA+CVI+SVNYRL+PE+RLPAAY+
Sbjct: 90  SPSVTLPLLVYFHGGGFCVGSAAWSCYHDFLTSLAVKARCVIVSVNYRLAPEHRLPAAYD 149

Query: 162 DGFNT---LIKHSI 168
           DG N    L+K  I
Sbjct: 150 DGVNVVSWLVKQQI 160

BLAST of CSPI04G08560.1 vs. Swiss-Prot
Match: CXE6_ARATH (Probable carboxylesterase 6 OS=Arabidopsis thaliana GN=CXE6 PE=2 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 3.2e-36
Identity = 70/127 (55.12%), Postives = 95/127 (74.80%), Query Frame = 1

Query: 42  EEIEGVIRVYADGHVERPPIIPNVSCSITASDKRVIAKDVLIENLTNTWARIYVA----- 101
           +E+EG+I+VY DGHVER  ++P V  S+   +  V   DV+I+ LTN WAR+YV      
Sbjct: 25  DEVEGLIKVYKDGHVERSQLLPCVDPSLPL-ELGVTCSDVVIDKLTNVWARLYVPMTTTK 84

Query: 102 TSQRNLPLLVYFHGGGFCVGSTAWSCYHEFLLDLASKAKCVILSVNYRLSPENRLPAAYE 161
           +S   LPL+VYFHGGGFCVGS +W CYHEFL  L+++++C+++SVNYRL+PEN LPAAYE
Sbjct: 85  SSVSKLPLIVYFHGGGFCVGSASWLCYHEFLARLSARSRCLVMSVNYRLAPENPLPAAYE 144

Query: 162 DGFNTLI 164
           DG N ++
Sbjct: 145 DGVNAIL 150

BLAST of CSPI04G08560.1 vs. Swiss-Prot
Match: TCEA1_TULGE (Tuliposide A-converting enzyme 1, chloroplastic OS=Tulipa gesneriana GN=TCEA1 PE=1 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 8.8e-18
Identity = 50/131 (38.17%), Postives = 77/131 (58.78%), Query Frame = 1

Query: 43  EIEGVIRVYADGHVERPPIIPNVSCSITASDKRVIAKDVLIENLTNTWARIY----VATS 102
           +++  + +Y  G +ER      +  ++  +   V  KDV+I+  T    R+Y    V   
Sbjct: 86  DLKPFLIIYKSGRIER-----FLGTTVIPACPEVATKDVVIDPATGVSVRLYLPNVVDLP 145

Query: 103 QRNLPLLVYFHGGGFCVGSTAWSCYHEFLLDLASKAKCVILSVNYRLSPENRLPAAYED- 162
            + LP+LVYFHGGGF + +T    YH +L  LA+KA  +I+S+NYRL+PE  LPA+Y+D 
Sbjct: 146 SKKLPVLVYFHGGGFVIENTGSPNYHNYLTLLAAKAGVLIVSINYRLAPEYPLPASYDDC 205

Query: 163 --GFNTLIKHS 167
             GFN ++ HS
Sbjct: 206 MAGFNWVVSHS 211

BLAST of CSPI04G08560.1 vs. Swiss-Prot
Match: TCEA2_TULGE (Tuliposide A-converting enzyme 2, chloroplastic OS=Tulipa gesneriana GN=TCEA2 PE=1 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 8.8e-18
Identity = 50/131 (38.17%), Postives = 77/131 (58.78%), Query Frame = 1

Query: 43  EIEGVIRVYADGHVERPPIIPNVSCSITASDKRVIAKDVLIENLTNTWARIY----VATS 102
           +++  + +Y  G +ER      +  ++  +   V  KDV+I+  T    R+Y    V   
Sbjct: 83  DLKPFLIIYKSGRIER-----FLGTTVIPACPEVATKDVVIDPATGVSVRLYLPNVVDLP 142

Query: 103 QRNLPLLVYFHGGGFCVGSTAWSCYHEFLLDLASKAKCVILSVNYRLSPENRLPAAYED- 162
            + LP+LVYFHGGGF + +T    YH +L  LA+KA  +I+S+NYRL+PE  LPA+Y+D 
Sbjct: 143 SKKLPVLVYFHGGGFVIENTGSPNYHNYLTLLAAKAGVLIVSINYRLAPEYPLPASYDDC 202

Query: 163 --GFNTLIKHS 167
             GFN ++ HS
Sbjct: 203 MAGFNWVVSHS 208

BLAST of CSPI04G08560.1 vs. Swiss-Prot
Match: TCEB4_TULGE (Probable tuliposide A-converting enzyme b6, amyloplastic OS=Tulipa gesneriana GN=TCEA-B6 PE=2 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 1.1e-17
Identity = 49/124 (39.52%), Postives = 74/124 (59.68%), Query Frame = 1

Query: 50  VYADGHVERPPIIPNVSCSITASDKRVIAKDVLIENLTNTWARIY----VATSQRNLPLL 109
           +Y DG +ER         ++  +   V  KDV+I+  T    R+Y    V    + LP+L
Sbjct: 84  IYKDGRIERLK-----GTTVIPACPEVATKDVIIDPATGVSVRLYLPNVVDLPSKKLPVL 143

Query: 110 VYFHGGGFCVGSTAWSCYHEFLLDLASKAKCVILSVNYRLSPENRLPAAYED---GFNTL 167
           VYFHGGGF + +T    YH +L  LA+K+  +I+SVNYRL+PE+ +PA+++D   GFN +
Sbjct: 144 VYFHGGGFVIENTGSPNYHNYLTLLAAKSGLLIVSVNYRLAPEHPIPASFDDCMAGFNWV 202

BLAST of CSPI04G08560.1 vs. TrEMBL
Match: F6HSF1_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_14s0006g03180 PE=4 SV=1)

HSP 1 Score: 199.9 bits (507), Expect = 4.3e-48
Identity = 97/148 (65.54%), Postives = 115/148 (77.70%), Query Frame = 1

Query: 17  MAAISIDPRLKLQIPKLRIQNEATTEEIEGVIRVYADGHVERPPIIPNVSCSITASDKRV 76
           MAAIS+DPRL LQ+ K   QN    E++EG+IRVY DGHVERP I+PNV C++ A +  V
Sbjct: 1   MAAISLDPRLSLQMGKNLYQNGVVVEKVEGLIRVYNDGHVERPAIVPNVPCTV-ALELGV 60

Query: 77  IAKDVLIENLTNTWARIYVATSQRN-LPLLVYFHGGGFCVGSTAWSCYHEFLLDLASKAK 136
             KDV+IE  +N WAR YV +     LPLLVYFHGGGFCVGS AW+CYH FL DLASKA 
Sbjct: 61  TVKDVVIEKYSNLWARFYVPSCPAGKLPLLVYFHGGGFCVGSAAWNCYHGFLADLASKAG 120

Query: 137 CVILSVNYRLSPENRLPAAYEDGFNTLI 164
           C+I+SVNYRL+PENRLPAAYEDGFN ++
Sbjct: 121 CLIMSVNYRLAPENRLPAAYEDGFNAVM 147

BLAST of CSPI04G08560.1 vs. TrEMBL
Match: A5C4X4_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_033324 PE=4 SV=1)

HSP 1 Score: 199.9 bits (507), Expect = 4.3e-48
Identity = 97/148 (65.54%), Postives = 115/148 (77.70%), Query Frame = 1

Query: 17  MAAISIDPRLKLQIPKLRIQNEATTEEIEGVIRVYADGHVERPPIIPNVSCSITASDKRV 76
           MAAIS+DPRL LQ+ K   QN    E++EG+IRVY DGHVERP I+PNV C++ A +  V
Sbjct: 1   MAAISLDPRLSLQMGKNLYQNGVVVEKVEGLIRVYNDGHVERPAIVPNVPCTV-ALELGV 60

Query: 77  IAKDVLIENLTNTWARIYVATSQRN-LPLLVYFHGGGFCVGSTAWSCYHEFLLDLASKAK 136
             KDV+IE  +N WAR YV +     LPLLVYFHGGGFCVGS AW+CYH FL DLASKA 
Sbjct: 61  TVKDVVIEKYSNLWARFYVPSCPAGKLPLLVYFHGGGFCVGSAAWNCYHGFLADLASKAG 120

Query: 137 CVILSVNYRLSPENRLPAAYEDGFNTLI 164
           C+I+SVNYRL+PENRLPAAYEDGFN ++
Sbjct: 121 CLIMSVNYRLAPENRLPAAYEDGFNAVM 147

BLAST of CSPI04G08560.1 vs. TrEMBL
Match: A0A0K9RJX2_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_058210 PE=4 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 7.4e-48
Identity = 94/148 (63.51%), Postives = 119/148 (80.41%), Query Frame = 1

Query: 17  MAAISIDPRLKLQIPKLRIQNEATTEEIEGVIRVYADGHVERPPIIPNVSCSITASDKRV 76
           MA++S+DPRL +        + A  EEI+G+IRV+ DGHVERPPII NV CSI  S+ RV
Sbjct: 1   MASLSLDPRLSVNA-SYHHHHGALVEEIQGLIRVHKDGHVERPPIITNVPCSIP-SELRV 60

Query: 77  IAKDVLIENLTNTWARIYVATSQRN-LPLLVYFHGGGFCVGSTAWSCYHEFLLDLASKAK 136
           +++D++I   TN WAR+YV   QRN LPL+VYFHGGGFCVGS AWSCYH+F+ +LASKA 
Sbjct: 61  LSRDIVINKFTNLWARVYVPQGQRNHLPLVVYFHGGGFCVGSAAWSCYHDFIANLASKAG 120

Query: 137 CVILSVNYRLSPENRLPAAYEDGFNTLI 164
           CV++SVNYRL+PENRLPAAYEDG+NT++
Sbjct: 121 CVVISVNYRLAPENRLPAAYEDGYNTIM 146

BLAST of CSPI04G08560.1 vs. TrEMBL
Match: B9RSL2_RICCO (Lipase, putative OS=Ricinus communis GN=RCOM_1726020 PE=4 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 9.1e-46
Identity = 95/148 (64.19%), Postives = 114/148 (77.03%), Query Frame = 1

Query: 17  MAAISIDPRLKLQIPKLRIQNEATTEEIEGVIRVYADGHVERPPIIPNVSCSITASDKRV 76
           MAAIS D RL LQ+ K    +    EEIEG+I+VY DG  ERPPI+PNV+C+  A +  V
Sbjct: 1   MAAISYDSRLHLQVGKAN--HGVLVEEIEGLIKVYRDGRTERPPIVPNVACA-PAPEDGV 60

Query: 77  IAKDVLIENLTNTWARIYVATSQ-RNLPLLVYFHGGGFCVGSTAWSCYHEFLLDLASKAK 136
            AKDV I+ LTN WARIY+ +     LPLLVYFHGGGFCVGS AW CYHEFL++LASKA 
Sbjct: 61  TAKDVFIDKLTNLWARIYLPSCPGTRLPLLVYFHGGGFCVGSAAWICYHEFLVNLASKAG 120

Query: 137 CVILSVNYRLSPENRLPAAYEDGFNTLI 164
           C+I+S+NYRL+PENRLPAAY+DG NTL+
Sbjct: 121 CIIISINYRLAPENRLPAAYDDGTNTLM 145

BLAST of CSPI04G08560.1 vs. TrEMBL
Match: M5WYE7_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa016280mg PE=4 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 1.2e-45
Identity = 94/148 (63.51%), Postives = 115/148 (77.70%), Query Frame = 1

Query: 17  MAAISIDPRLKLQ-IPKLRIQNEATTEEIEGVIRVYADGHVERPPIIPNVSCSITASDKR 76
           M+ IS+DPRL LQ I     Q+    EEIEG++RV+ DG++ERPPIIP+V  ++ A    
Sbjct: 1   MSTISLDPRLNLQAIKNPPNQHGVVLEEIEGLLRVHKDGNIERPPIIPSVPSTL-ALPHG 60

Query: 77  VIAKDVLIENLTNTWARIYVATSQRNLPLLVYFHGGGFCVGSTAWSCYHEFLLDLASKAK 136
           V AKDV+I+  TN WARIYV +   NLP+LVYFHGGGFCVGS AWSCYH+FL +LA KA 
Sbjct: 61  VTAKDVVIDKFTNLWARIYVPSHPGNLPVLVYFHGGGFCVGSAAWSCYHDFLANLACKAS 120

Query: 137 CVILSVNYRLSPENRLPAAYEDGFNTLI 164
           CVI+SVNYRL+PENRLPAAY+DGF TL+
Sbjct: 121 CVIISVNYRLAPENRLPAAYDDGFKTLM 147

BLAST of CSPI04G08560.1 vs. TAIR10
Match: AT5G16080.1 (AT5G16080.1 carboxyesterase 17)

HSP 1 Score: 156.0 bits (393), Expect = 3.6e-38
Identity = 80/134 (59.70%), Postives = 98/134 (73.13%), Query Frame = 1

Query: 42  EEIEGVIRVYADGHVERPPIIPNVSCSITASDKRVIAKDVLIENLTNTWARIYV-----A 101
           EEIEG+I+V+ DG VERPPI+P VS +I  S K   A D+ + N  +TW R+Y+     A
Sbjct: 30  EEIEGLIKVFNDGCVERPPIVPIVSPTIHPSSKAT-AFDIKLSN--DTWTRVYIPDAAAA 89

Query: 102 TSQRNLPLLVYFHGGGFCVGSTAWSCYHEFLLDLASKAKCVILSVNYRLSPENRLPAAYE 161
           +    LPLLVYFHGGGFCVGS AWSCYH+FL  LA KA+CVI+SVNYRL+PE+RLPAAY+
Sbjct: 90  SPSVTLPLLVYFHGGGFCVGSAAWSCYHDFLTSLAVKARCVIVSVNYRLAPEHRLPAAYD 149

Query: 162 DGFNT---LIKHSI 168
           DG N    L+K  I
Sbjct: 150 DGVNVVSWLVKQQI 160

BLAST of CSPI04G08560.1 vs. TAIR10
Match: AT1G68620.1 (AT1G68620.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 153.7 bits (387), Expect = 1.8e-37
Identity = 70/127 (55.12%), Postives = 95/127 (74.80%), Query Frame = 1

Query: 42  EEIEGVIRVYADGHVERPPIIPNVSCSITASDKRVIAKDVLIENLTNTWARIYVA----- 101
           +E+EG+I+VY DGHVER  ++P V  S+   +  V   DV+I+ LTN WAR+YV      
Sbjct: 25  DEVEGLIKVYKDGHVERSQLLPCVDPSLPL-ELGVTCSDVVIDKLTNVWARLYVPMTTTK 84

Query: 102 TSQRNLPLLVYFHGGGFCVGSTAWSCYHEFLLDLASKAKCVILSVNYRLSPENRLPAAYE 161
           +S   LPL+VYFHGGGFCVGS +W CYHEFL  L+++++C+++SVNYRL+PEN LPAAYE
Sbjct: 85  SSVSKLPLIVYFHGGGFCVGSASWLCYHEFLARLSARSRCLVMSVNYRLAPENPLPAAYE 144

Query: 162 DGFNTLI 164
           DG N ++
Sbjct: 145 DGVNAIL 150

BLAST of CSPI04G08560.1 vs. TAIR10
Match: AT5G06570.1 (AT5G06570.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 91.3 bits (225), Expect = 1.1e-18
Identity = 47/120 (39.17%), Postives = 74/120 (61.67%), Query Frame = 1

Query: 42  EEIEGVIRVYADGHVERPPIIPNVSCSIT-ASDKRVIAKDVLIENLTNTWARIYVATSQR 101
           E+  G++++ ++G V R   I  ++  I   +++ V+ KD +     N   R+Y   S  
Sbjct: 12  EDCMGLLQLLSNGTVLRSESIDLITQQIPFKNNQTVLFKDSIYHKPNNLHLRLYKPISAS 71

Query: 102 N---LPLLVYFHGGGFCVGSTAWSCYHEFLLDLASKAKCVILSVNYRLSPENRLPAAYED 158
           N   LP++V+FHGGGFC GS +W  +H F L LAS    +++S +YRL+PE+RLPAA+ED
Sbjct: 72  NRTALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSLNALVVSPDYRLAPEHRLPAAFED 131

BLAST of CSPI04G08560.1 vs. TAIR10
Match: AT5G62180.1 (AT5G62180.1 carboxyesterase 20)

HSP 1 Score: 86.7 bits (213), Expect = 2.7e-17
Identity = 43/122 (35.25%), Postives = 69/122 (56.56%), Query Frame = 1

Query: 53  DGHVERPPIIPNVSCSITASDKR----VIAKDVLIENLTNTWARIYVATSQRN------- 112
           DG + R   + N  C+    D       ++KD+ +  L +TW R+Y+ +S  N       
Sbjct: 21  DGSITRD--LSNFPCTAATPDPSPLNPAVSKDLPVNQLKSTWLRLYLPSSAVNEGNVSSQ 80

Query: 113 -LPLLVYFHGGGFCVGSTAWSCYHEFLLDLASKAKCVILSVNYRLSPENRLPAAYEDGFN 163
            LP++VY+HGGGF + S     +H+F  ++A     +++S +YRL+PE+RLPAAY+DG  
Sbjct: 81  KLPIVVYYHGGGFILCSVDMQLFHDFCSEVARDLNAIVVSPSYRLAPEHRLPAAYDDGVE 140

BLAST of CSPI04G08560.1 vs. TAIR10
Match: AT1G47480.1 (AT1G47480.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 84.7 bits (208), Expect = 1.0e-16
Identity = 49/119 (41.18%), Postives = 69/119 (57.98%), Query Frame = 1

Query: 50  VYADGHVER---PPIIPNVSCSITASDKRVIAKDVLIENLTNTWARIYVATS---QRNLP 109
           V+ DG VER     + P     IT     V +KD++IE  T   ARIY   S    + +P
Sbjct: 18  VHTDGTVERLAGTEVCPPGLDPITG----VFSKDIIIEPKTGLSARIYRPFSIQPGQKIP 77

Query: 110 LLVYFHGGGFCVGSTAWSCYHEFLLDLASKAKCVILSVNYRLSPENRLPAAYEDGFNTL 163
           L++YFHGG F + ST++  YH  L  + ++A  + +SVNYRL+PE+ LP AYED +  L
Sbjct: 78  LMLYFHGGAFLISSTSFPSYHTSLNKIVNQANVIAVSVNYRLAPEHPLPTAYEDSWTAL 132

BLAST of CSPI04G08560.1 vs. NCBI nr
Match: gi|778697555|ref|XP_011654347.1| (PREDICTED: LOW QUALITY PROTEIN: probable carboxylesterase 17 [Cucumis sativus])

HSP 1 Score: 285.0 bits (728), Expect = 1.5e-73
Identity = 142/163 (87.12%), Postives = 145/163 (88.96%), Query Frame = 1

Query: 1   MPNHHSRLIYSKKLKRMAAISIDPRLKLQIPKLRIQNEATTEEIEGVIRVYADGHVERPP 60
           MPNH       KKLKRM  ISIDPRLKLQIPKL IQNEATTEEIEGVIRVYADG VERPP
Sbjct: 1   MPNH------CKKLKRMTTISIDPRLKLQIPKLCIQNEATTEEIEGVIRVYADGRVERPP 60

Query: 61  IIPNVSCSITASDKRVIAKDVLIENLTNTWARIYVATSQRNLPLLVYFHGGGFCVGSTAW 120
           IIPNVSC IT S+KRVIAKDVLIEN TNTWARIYVATSQ NLPLLVYFHG GFCVGS AW
Sbjct: 61  IIPNVSCGITTSNKRVIAKDVLIENFTNTWARIYVATSQXNLPLLVYFHGDGFCVGSAAW 120

Query: 121 SCYHEFLLDLASKAKCVILSVNYRLSPENRLPAAYEDGFNTLI 164
           SCYHEFLLDLASKAKC+ILSVNYRLSPEN LPAAYEDGFNTL+
Sbjct: 121 SCYHEFLLDLASKAKCIILSVNYRLSPENHLPAAYEDGFNTLM 157

BLAST of CSPI04G08560.1 vs. NCBI nr
Match: gi|719967928|ref|XP_010263304.1| (PREDICTED: probable carboxylesterase 17 [Nelumbo nucifera])

HSP 1 Score: 201.4 bits (511), Expect = 2.1e-48
Identity = 93/147 (63.27%), Postives = 115/147 (78.23%), Query Frame = 1

Query: 17  MAAISIDPRLKLQIPKLRIQNEATTEEIEGVIRVYADGHVERPPIIPNVSCSITASDKRV 76
           MAAIS+DPR+ L I   R Q+   T+EIEG+I VY DGH+ERP ++PNV+C++ A    V
Sbjct: 1   MAAISVDPRMSLSIGHDRRQHGIVTDEIEGIITVYKDGHLERPLVVPNVACTV-APQPHV 60

Query: 77  IAKDVLIENLTNTWARIYVATSQRNLPLLVYFHGGGFCVGSTAWSCYHEFLLDLASKAKC 136
            ++DV+I  ++N WAR YV   QR LPLLVYFHGGGFCVGS AW CYHEFL +LAS+A C
Sbjct: 61  TSRDVIINKVSNVWARFYVPRCQRKLPLLVYFHGGGFCVGSAAWKCYHEFLANLASRAGC 120

Query: 137 VILSVNYRLSPENRLPAAYEDGFNTLI 164
           +I+SVNYRL+PENRLPAAYEDG N L+
Sbjct: 121 LIMSVNYRLAPENRLPAAYEDGCNALL 146

BLAST of CSPI04G08560.1 vs. NCBI nr
Match: gi|147836555|emb|CAN75310.1| (hypothetical protein VITISV_033324 [Vitis vinifera])

HSP 1 Score: 199.9 bits (507), Expect = 6.2e-48
Identity = 97/148 (65.54%), Postives = 115/148 (77.70%), Query Frame = 1

Query: 17  MAAISIDPRLKLQIPKLRIQNEATTEEIEGVIRVYADGHVERPPIIPNVSCSITASDKRV 76
           MAAIS+DPRL LQ+ K   QN    E++EG+IRVY DGHVERP I+PNV C++ A +  V
Sbjct: 1   MAAISLDPRLSLQMGKNLYQNGVVVEKVEGLIRVYNDGHVERPAIVPNVPCTV-ALELGV 60

Query: 77  IAKDVLIENLTNTWARIYVATSQRN-LPLLVYFHGGGFCVGSTAWSCYHEFLLDLASKAK 136
             KDV+IE  +N WAR YV +     LPLLVYFHGGGFCVGS AW+CYH FL DLASKA 
Sbjct: 61  TVKDVVIEKYSNLWARFYVPSCPAGKLPLLVYFHGGGFCVGSAAWNCYHGFLADLASKAG 120

Query: 137 CVILSVNYRLSPENRLPAAYEDGFNTLI 164
           C+I+SVNYRL+PENRLPAAYEDGFN ++
Sbjct: 121 CLIMSVNYRLAPENRLPAAYEDGFNAVM 147

BLAST of CSPI04G08560.1 vs. NCBI nr
Match: gi|731417830|ref|XP_002279965.2| (PREDICTED: probable carboxylesterase 17 [Vitis vinifera])

HSP 1 Score: 199.9 bits (507), Expect = 6.2e-48
Identity = 97/148 (65.54%), Postives = 115/148 (77.70%), Query Frame = 1

Query: 17  MAAISIDPRLKLQIPKLRIQNEATTEEIEGVIRVYADGHVERPPIIPNVSCSITASDKRV 76
           MAAIS+DPRL LQ+ K   QN    E++EG+IRVY DGHVERP I+PNV C++ A +  V
Sbjct: 6   MAAISLDPRLSLQMGKNLYQNGVVVEKVEGLIRVYNDGHVERPAIVPNVPCTV-ALELGV 65

Query: 77  IAKDVLIENLTNTWARIYVATSQRN-LPLLVYFHGGGFCVGSTAWSCYHEFLLDLASKAK 136
             KDV+IE  +N WAR YV +     LPLLVYFHGGGFCVGS AW+CYH FL DLASKA 
Sbjct: 66  TVKDVVIEKYSNLWARFYVPSCPAGKLPLLVYFHGGGFCVGSAAWNCYHGFLADLASKAG 125

Query: 137 CVILSVNYRLSPENRLPAAYEDGFNTLI 164
           C+I+SVNYRL+PENRLPAAYEDGFN ++
Sbjct: 126 CLIMSVNYRLAPENRLPAAYEDGFNAVM 152

BLAST of CSPI04G08560.1 vs. NCBI nr
Match: gi|902224477|gb|KNA19795.1| (hypothetical protein SOVF_058210 [Spinacia oleracea])

HSP 1 Score: 199.1 bits (505), Expect = 1.1e-47
Identity = 94/148 (63.51%), Postives = 119/148 (80.41%), Query Frame = 1

Query: 17  MAAISIDPRLKLQIPKLRIQNEATTEEIEGVIRVYADGHVERPPIIPNVSCSITASDKRV 76
           MA++S+DPRL +        + A  EEI+G+IRV+ DGHVERPPII NV CSI  S+ RV
Sbjct: 1   MASLSLDPRLSVNA-SYHHHHGALVEEIQGLIRVHKDGHVERPPIITNVPCSIP-SELRV 60

Query: 77  IAKDVLIENLTNTWARIYVATSQRN-LPLLVYFHGGGFCVGSTAWSCYHEFLLDLASKAK 136
           +++D++I   TN WAR+YV   QRN LPL+VYFHGGGFCVGS AWSCYH+F+ +LASKA 
Sbjct: 61  LSRDIVINKFTNLWARVYVPQGQRNHLPLVVYFHGGGFCVGSAAWSCYHDFIANLASKAG 120

Query: 137 CVILSVNYRLSPENRLPAAYEDGFNTLI 164
           CV++SVNYRL+PENRLPAAYEDG+NT++
Sbjct: 121 CVVISVNYRLAPENRLPAAYEDGYNTIM 146

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
CXE17_ARATH6.5e-3759.70Probable carboxylesterase 17 OS=Arabidopsis thaliana GN=CXE17 PE=2 SV=1[more]
CXE6_ARATH3.2e-3655.12Probable carboxylesterase 6 OS=Arabidopsis thaliana GN=CXE6 PE=2 SV=1[more]
TCEA1_TULGE8.8e-1838.17Tuliposide A-converting enzyme 1, chloroplastic OS=Tulipa gesneriana GN=TCEA1 PE... [more]
TCEA2_TULGE8.8e-1838.17Tuliposide A-converting enzyme 2, chloroplastic OS=Tulipa gesneriana GN=TCEA2 PE... [more]
TCEB4_TULGE1.1e-1739.52Probable tuliposide A-converting enzyme b6, amyloplastic OS=Tulipa gesneriana GN... [more]
Match NameE-valueIdentityDescription
F6HSF1_VITVI4.3e-4865.54Putative uncharacterized protein OS=Vitis vinifera GN=VIT_14s0006g03180 PE=4 SV=... [more]
A5C4X4_VITVI4.3e-4865.54Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_033324 PE=4 SV=1[more]
A0A0K9RJX2_SPIOL7.4e-4863.51Uncharacterized protein OS=Spinacia oleracea GN=SOVF_058210 PE=4 SV=1[more]
B9RSL2_RICCO9.1e-4664.19Lipase, putative OS=Ricinus communis GN=RCOM_1726020 PE=4 SV=1[more]
M5WYE7_PRUPE1.2e-4563.51Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa016280mg PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G16080.13.6e-3859.70 carboxyesterase 17[more]
AT1G68620.11.8e-3755.12 alpha/beta-Hydrolases superfamily protein[more]
AT5G06570.11.1e-1839.17 alpha/beta-Hydrolases superfamily protein[more]
AT5G62180.12.7e-1735.25 carboxyesterase 20[more]
AT1G47480.11.0e-1641.18 alpha/beta-Hydrolases superfamily protein[more]
Match NameE-valueIdentityDescription
gi|778697555|ref|XP_011654347.1|1.5e-7387.12PREDICTED: LOW QUALITY PROTEIN: probable carboxylesterase 17 [Cucumis sativus][more]
gi|719967928|ref|XP_010263304.1|2.1e-4863.27PREDICTED: probable carboxylesterase 17 [Nelumbo nucifera][more]
gi|147836555|emb|CAN75310.1|6.2e-4865.54hypothetical protein VITISV_033324 [Vitis vinifera][more]
gi|731417830|ref|XP_002279965.2|6.2e-4865.54PREDICTED: probable carboxylesterase 17 [Vitis vinifera][more]
gi|902224477|gb|KNA19795.1|1.1e-4763.51hypothetical protein SOVF_058210 [Spinacia oleracea][more]
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
IPR013094AB_hydrolase_3
Vocabulary: Biological Process
TermDefinition
GO:0008152metabolic process
Vocabulary: Molecular Function
TermDefinition
GO:0016787hydrolase activity
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008152 metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0016787 hydrolase activity

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CSPI04G08560CSPI04G08560gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CSPI04G08560.1CSPI04G08560.1-proteinpolypeptide


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CSPI04G08560.1.cds2CSPI04G08560.1.cds2CDS
CSPI04G08560.1.cds1CSPI04G08560.1.cds1CDS


Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013094Alpha/beta hydrolase fold-3PFAMPF07859Abhydrolase_3coord: 105..162
score: 5.2
NoneNo IPR availablePANTHERPTHR23024MEMBER OF 'GDXG' FAMILY OF LIPOLYTIC ENZYMEScoord: 16..162
score: 2.2
NoneNo IPR availablePANTHERPTHR23024:SF93CARBOXYLESTERASE 17-RELATEDcoord: 16..162
score: 2.2