CSPI03G25040.1 (mRNA) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI03G25040.1
Type	mRNA
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	Pectinesterase
Location	Chr3 : 22258909 .. 22261377 (+)
Sequence length	1131

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAACATTATTATTCGAAAGAGATAAGTTTGTTGATTATAACGACGTTGGTGGTGTTGTTTTGTGTGAAAGTTTCCAAGGCATTGGATGATTGTGAATTGAATTATAATGATCCTCATAAAGTTGCATACACTATTGTTGTTGATAAACTTGGGAAAGGTAACTTCAACACAATCCAAAGTGCCATTAATTCTGTCCCTTCCCAAAGTACCAAATGGATTAGAATTCAAGTTTCTCCTGGCATATATACGTACGGTATACGATCTTATTTCCCGTTTATTAAAATATCATTTTAGTTCCCTTGTTTGATTTCATTTTATTTTCATCAAGTTTAGATTTAGTTTTTCTTTTTCTTTTTTCCATACCTACTAAAGTCTATCACTGTTTGATTTCTATCAGCATTTATTAGTGATAGGTGATAGTTATAGAAACATGAATAGTCTAATTTGGTTAGCATGTTTATGTCGAACATTCGTTTAGTGTAACAAATGAATTAGACATGCATAGAACCTTTATATTGAGGTATATACTAAAAGAGACACTACGTGACAATAATAACTTTTGAGCATGTGATACATCAAGCAAAGTTTTTTAAGCATATAAATGTATCAATGCATATGTTATGAAGTTTCTTTTACTATAAGAATGATATTATATTTTTAAGAAAGAATATTTTAATAAATGTGTAATCATCATGTCGTGTCCTAGATTTTTAAATAATGGCGTGTTGTGGTGTCCCTATTGTGCCATATCCATGTCTCAAATTTGAATTTGTGCTATTAGGATCGGATGACAGTAGTCTTGTTTATCGTCTATCAATAATATTTTGTTATATATACTTGGAATCAATTAAAACAACCATTACACATGTTATATTTTAACAGGGAAAAAGTGACGATACCCTCCGAAAAATCATGCATATTTCTAGATGGAAGTGGGCTTCAAGTGACTGAAATTCATTGGAATGACCATGAAACAACAGCTGCTAGTCCCACCTTTACTGCTTCTGCACAAAACCTTGTAGTACAAGGCATCACTTTTAGGGTATATACATATAACTGTTTAATTTCCCTAACTTTCAAATAATGTATATATTTTCTTTGTTTTATGTCTATTATCTGTGATCCAAAATTGGCAGAATACTTATAATGCACGTGGAAGTGTGATGAGACGTGAAGATATAAAACCAGCACTTGCAGCTCTAATCCAAGGAGACAAAGTGATATTTCATAAATGTGGTTTCATTGGTTTGCAAGATACTTTGTGGGATGGTCCTGGCCGTCATCTATTCACCCAGTGCTACATTGAAGGTGTCATTGATGTCATTTCTGGTTTTGGTCAGTCTATCTATAAGGTTAACTTCTAAATTAATTATAAGCTTACATGTGTGTTTGTATATGTGTGAGAGCTTGGAAGTGAAATTGAAGTGTTGAGTTTTTCTTTTCTGTTTGTTTGTTTGTTGTGTTTAACGCAGGAATGTGTGATAAACATACCAGTCAATGCATATGCTCCATTACTAAATGAAGGTTTCATAACAGCTCAAGGAAAGGAGAATCCAAATGAAAGCAGTGGATTTGTATTCCTTAGATGCATAGTGCAGGGAAGTGGGAATGTTTTTCTTGGAAGGGCTTATAGACCTTTCTCCACTGTCATTTTCCATCTCTGTTTTCTACCTTCTTGCATTAACCCTGCTGGTTGGAATTCTTGGCTACAGGCTGGGCATGAGTAATAATTAACTCTTCTTTTCATCTTTTTCATTTCATTTTATATAGCATATTATTAAAGTATCATTATTATCATTATTATCATTATTATCATTATTTGTTTGTAGGAGTGATTTGACGTATTCAGAGACCCGTTGTACTGGGCCAGGAGCAGATACTTCAAGCCGTGTGCCTTGGGTTAATAGATTGGATGCATTTCACATAAGAAGCTTCACAGATATTTCTTATATTGATCCACAAGGTTATTGGACTTCAAGGATTCCTCTTTTATCCTAATTAATTTAATTAAGTTTCTAAACTTAATTAATGGGCCCTTTGTAATTCTTTTCTATTCTCTCAGTAAATAATGAAAAATACTTTTCTTTTTTCAGTCATTTTAATCTTTCCTTCTACTGAAGCTTCAAATTGACTTTTTTTTTTTTCTTGCTTAGAGATTGCTAATATAACATGTAGGGTTTAAGTTAGAAAGATTTAAAAAACTAGTTGTTGGAGGGGAGCGCCAAGGCCCAAGCAGTAGTGCAACGCAAGGGCGACGCTCGAAGGCCCCAATAGCAAGCTATTGTGATGGGCCCTCCCCCTAGAAAAATAAATTTAATATCATGTGAGCCGATGGCCCACGTATCAGATATTAAACGGATAAGAACAGATACTACACTTGATCTTAGCCAAAAGGCCGAGAAAGGTATGCTTTTAGAGTCCAATGAAACTCATGTTTATACTCCGCCGCTTGCTTCCATTTTGTGA

mRNA sequence

ATGGAACATTATTATTCGAAAGAGATAAGTTTGTTGATTATAACGACGTTGGTGGTGTTGTTTTGTGTGAAAGTTTCCAAGGCATTGGATGATTGTGAATTGAATTATAATGATCCTCATAAAGTTGCATACACTATTGTTGTTGATAAACTTGGGAAAGGTAACTTCAACACAATCCAAAGTGCCATTAATTCTGTCCCTTCCCAAAGTACCAAATGGATTAGAATTCAAGTTTCTCCTGGCATATATACGGAAAAAGTGACGATACCCTCCGAAAAATCATGCATATTTCTAGATGGAAGTGGGCTTCAAGTGACTGAAATTCATTGGAATGACCATGAAACAACAGCTGCTAGTCCCACCTTTACTGCTTCTGCACAAAACCTTGTAGTACAAGGCATCACTTTTAGGAATACTTATAATGCACGTGGAAGTGTGATGAGACGTGAAGATATAAAACCAGCACTTGCAGCTCTAATCCAAGGAGACAAAGTGATATTTCATAAATGTGGTTTCATTGGTTTGCAAGATACTTTGTGGGATGGTCCTGGCCGTCATCTATTCACCCAGTGCTACATTGAAGGTGTCATTGATGTCATTTCTGGTTTTGGTCAGTCTATCTATAAGGAATGTGTGATAAACATACCAGTCAATGCATATGCTCCATTACTAAATGAAGGTTTCATAACAGCTCAAGGAAAGGAGAATCCAAATGAAAGCAGTGGATTTGTATTCCTTAGATGCATAGTGCAGGGAAGTGGGAATGTTTTTCTTGGAAGGGCTTATAGACCTTTCTCCACTGTCATTTTCCATCTCTGTTTTCTACCTTCTTGCATTAACCCTGCTGGTTGGAATTCTTGGCTACAGGCTGGGCATGAGAGTGATTTGACGTATTCAGAGACCCGTTGTACTGGGCCAGGAGCAGATACTTCAAGCCGTGTGCCTTGGGTTAATAGATTGGATGCATTTCACATAAGAAGCTTCACAGATATTTCTTATATTGATCCACAAGATATTAAACGGATAAGAACAGATACTACACTTGATCTTAGCCAAAAGGCCGAGAAAGGTATGCTTTTAGAGTCCAATGAAACTCATGTTTATACTCCGCCGCTTGCTTCCATTTTGTGA

Coding sequence (CDS)

ATGGAACATTATTATTCGAAAGAGATAAGTTTGTTGATTATAACGACGTTGGTGGTGTTGTTTTGTGTGAAAGTTTCCAAGGCATTGGATGATTGTGAATTGAATTATAATGATCCTCATAAAGTTGCATACACTATTGTTGTTGATAAACTTGGGAAAGGTAACTTCAACACAATCCAAAGTGCCATTAATTCTGTCCCTTCCCAAAGTACCAAATGGATTAGAATTCAAGTTTCTCCTGGCATATATACGGAAAAAGTGACGATACCCTCCGAAAAATCATGCATATTTCTAGATGGAAGTGGGCTTCAAGTGACTGAAATTCATTGGAATGACCATGAAACAACAGCTGCTAGTCCCACCTTTACTGCTTCTGCACAAAACCTTGTAGTACAAGGCATCACTTTTAGGAATACTTATAATGCACGTGGAAGTGTGATGAGACGTGAAGATATAAAACCAGCACTTGCAGCTCTAATCCAAGGAGACAAAGTGATATTTCATAAATGTGGTTTCATTGGTTTGCAAGATACTTTGTGGGATGGTCCTGGCCGTCATCTATTCACCCAGTGCTACATTGAAGGTGTCATTGATGTCATTTCTGGTTTTGGTCAGTCTATCTATAAGGAATGTGTGATAAACATACCAGTCAATGCATATGCTCCATTACTAAATGAAGGTTTCATAACAGCTCAAGGAAAGGAGAATCCAAATGAAAGCAGTGGATTTGTATTCCTTAGATGCATAGTGCAGGGAAGTGGGAATGTTTTTCTTGGAAGGGCTTATAGACCTTTCTCCACTGTCATTTTCCATCTCTGTTTTCTACCTTCTTGCATTAACCCTGCTGGTTGGAATTCTTGGCTACAGGCTGGGCATGAGAGTGATTTGACGTATTCAGAGACCCGTTGTACTGGGCCAGGAGCAGATACTTCAAGCCGTGTGCCTTGGGTTAATAGATTGGATGCATTTCACATAAGAAGCTTCACAGATATTTCTTATATTGATCCACAAGATATTAAACGGATAAGAACAGATACTACACTTGATCTTAGCCAAAAGGCCGAGAAAGGTATGCTTTTAGAGTCCAATGAAACTCATGTTTATACTCCGCCGCTTGCTTCCATTTTGTGA

Homology

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BLAST of CSPI03G25040.1 vs. Swiss-Prot
Match: PME66_ARATH (Probable pectinesterase 66 OS=Arabidopsis thaliana GN=PME66 PE=2 SV=2)

HSP 1 Score: 290.8 bits (743), Expect = 2.1e-77
Identity = 138/295 (46.78%), Postives = 188/295 (63.73%), Query Frame = 1


Query: 41  KVAYTIVVDKLGKGNFNTIQSAINSVPSQSTKWIRIQVSPGIYTEKVTIPSEKSCIFLDG 100
           ++AYTI VD  G GNF T+QSAI+S+   +  WIR+    GIY EKVTIP EK  I+L G
Sbjct: 30  QIAYTITVDLNGGGNFTTVQSAIDSISPPNHNWIRVFTQNGIYREKVTIPKEKGFIYLQG 89

Query: 101 SGLQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARGSVMRREDIKPALAALI 160
            G++ T I ++DH+ T  S TFTA A ++V+ GITF+NTYN   +   + +I PA+AA +
Sbjct: 90  KGIEQTVIEYDDHQATDISATFTAFADDIVISGITFKNTYNIVPN--NKREIVPAVAARM 149

Query: 161 QGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAY 220
            GD+ +     F+GLQDTL+DG GRH + +C I G ID I G+GQS++KEC +N+ +  Y
Sbjct: 150 LGDRYVVTDSSFVGLQDTLFDGKGRHYYKRCIISGGIDFIFGYGQSLFKECTLNMTLGIY 209

Query: 221 APLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCIN 280
           AP    G ITA  + +P++  GFVF  C V G G   LGRA+   + VIF    L   + 
Sbjct: 210 APDNPYGTITAHQRPSPSDEGGFVFSDCTVTGVGKTLLGRAWGSNARVIFDRSRLSDVVL 269

Query: 281 PAGWNSWLQAGHESDLTYSETRCTGPGADTSSRVPWVNRLDAFHIRSFTDISYID 336
           P GW++W   G+E DLT+ E  CTG GADTS RVPW+ +L    +  F  +S+ID
Sbjct: 270 PIGWDAWRAKGNERDLTFVEAGCTGAGADTSQRVPWLKKLSLSEVDGFASVSFID 322

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BLAST of CSPI03G25040.1 vs. Swiss-Prot
Match: PME55_ARATH (Probable pectinesterase 55 OS=Arabidopsis thaliana GN=PME55 PE=2 SV=1)

HSP 1 Score: 290.8 bits (743), Expect = 2.1e-77
Identity = 138/302 (45.70%), Postives = 192/302 (63.58%), Query Frame = 1


Query: 39  PHKVA---YTIVVDKLGKGNFNTIQSAINSVPSQSTKWIRIQVSPGIYTEKVTIPSEKSC 98
           PH +    + ++VD+ G GNF TIQ AI+SVP  +T W  I V  G+Y EK+TIP +K  
Sbjct: 19  PHLIEAKPFEVIVDQSGHGNFTTIQKAIDSVPINNTHWFFINVKAGLYREKITIPQKKPF 78

Query: 99  IFLDGSGLQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYN--ARGSVMRREDIK 158
           I + G+G + T + W+DH + A SPTF   A N VV+ ITF N+YN  + G + +   + 
Sbjct: 79  IVIVGAGKRSTRVEWDDHASLAQSPTFATLADNTVVKKITFANSYNFPSNGKINKNPRV- 138

Query: 159 PALAALIQGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIYKECVI 218
           PA+AA I GDK  F+  GF G+QDTLWD  GRH F +C I+G +D I G GQSIY+ CVI
Sbjct: 139 PAVAAFIGGDKSAFYSVGFAGIQDTLWDSDGRHYFHRCTIQGAVDFILGSGQSIYQSCVI 198

Query: 219 NIPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLC 278
            +      P +  G+ITAQG+ N N+++GFVF+ C+V G G  +LGRA+RP+S VIF+  
Sbjct: 199 QVLGGQLGPGVT-GYITAQGRTNANDANGFVFINCLVHGFGKAYLGRAWRPYSRVIFYNS 258

Query: 279 FLPSCINPAGWNSWLQAGHESDLTYSETRCTGPGADTSSRVPWVNRLDAFHIRSFTDISY 336
            L   ++P GW  W   G+E  LTY+E  C G G++TS R  WV +L A  ++   D+S+
Sbjct: 259 NLTDVVDPLGWWEWNYQGYEKQLTYAEHGCFGSGSNTSRRAKWVKKLSASAVQHLADLSF 318

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BLAST of CSPI03G25040.1 vs. Swiss-Prot
Match: PME29_ARATH (Probable pectinesterase 29 OS=Arabidopsis thaliana GN=PME29 PE=2 SV=1)

HSP 1 Score: 281.6 bits (719), Expect = 1.3e-74
Identity = 136/292 (46.58%), Postives = 187/292 (64.04%), Query Frame = 1


Query: 46  IVVDKLGKGNFNTIQSAINSVPSQSTKWIRIQVSPGIYTEKVTIPSEKSCIFLDGSGLQV 105
           + VD+ G GNF TIQ AI+SVP  +  W  I V  G+Y EK+ IP EK  I L G+G ++
Sbjct: 34  VFVDQSGHGNFTTIQKAIDSVPINNRHWFFINVKAGLYREKIKIPYEKPFIVLVGAGKRL 93

Query: 106 TEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYN--ARGSVMRREDIKPALAALIQGD 165
           T + W+DH + A SPTF+  A N VV+ ITF N+YN  ++G  M +    PA+AALI GD
Sbjct: 94  TRVEWDDHYSVAQSPTFSTLADNTVVKSITFANSYNFPSKGK-MNKNPRTPAVAALIGGD 153

Query: 166 KVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAYAPL 225
           K  F+  GF G+QDTLWD  GRH F +C I+G +D I G GQSIY+ CVI +      P 
Sbjct: 154 KSAFYSVGFAGIQDTLWDFDGRHYFHRCTIQGAVDFIFGTGQSIYQSCVIQVLGGQLEPG 213

Query: 226 LNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCINPAG 285
           L  G+ITAQG+ NP +++GF+F+ C+V G+G  FLGR +R +S VIF+   L   + P G
Sbjct: 214 L-AGYITAQGRTNPYDANGFIFINCLVYGTGMAFLGRPWRGYSRVIFYNSNLTDVVVPEG 273

Query: 286 WNSWLQAGHESDLTYSETRCTGPGADTSSRVPWVNRLDAFHIRSFTDISYID 336
           W++W   GHE+ L ++E  C G GA+   RV WV +L    I++  D+S+I+
Sbjct: 274 WDAWNFVGHENQLVFAEHGCFGSGANIGRRVKWVKKLSESAIQNLADLSFIN 323

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BLAST of CSPI03G25040.1 vs. Swiss-Prot
Match: PME10_ARATH (Putative pectinesterase 10 OS=Arabidopsis thaliana GN=PME10 PE=2 SV=1)

HSP 1 Score: 270.0 bits (689), Expect = 3.9e-71
Identity = 130/297 (43.77%), Postives = 181/297 (60.94%), Query Frame = 1


Query: 41  KVAYTIVVDKLGKGNFNTIQSAINSVPSQSTKWIRIQVSPGIYTEKVTIPSEKSCIFLDG 100
           ++A TI+V+      F T+QSAI+S+P Q+  WIRI +S GIY+EKVTIP  K  I++ G
Sbjct: 36  QIAKTIIVNPNDARYFKTVQSAIDSIPLQNQDWIRILISNGIYSEKVTIPRGKGYIYMQG 95

Query: 101 SGLQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARGSVMRREDIKPALAALI 160
            G++ T I + DH+ T  S TFT+   N+++ GITF+N YN   S       KPA+AA++
Sbjct: 96  GGIEKTIIAYGDHQLTNTSATFTSYPSNIIITGITFKNKYNIASS---SSPTKPAVAAMM 155

Query: 161 QGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAY 220
            GDK       F G QDTL+D  GRH + +C I G ID I G  QSI++ C + + V  Y
Sbjct: 156 LGDKYAIIDSSFDGFQDTLYDDYGRHYYKRCVISGGIDFIFGGAQSIFEGCTLKLRVGIY 215

Query: 221 APLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCIN 280
            P    G ITAQG+++P +  GFVF  C V GSG   LGRA++ +S VIF+       I 
Sbjct: 216 PPNEVYGTITAQGRDSPTDKGGFVFKDCTVMGSGKALLGRAWKSYSRVIFYRSMFSDNIL 275

Query: 281 PAGWNSWLQAGHESDLTYSETRCTGPGADTSSRVPWVNRLDAFHIRSFTDISYIDPQ 338
           P GW++W   G E  +T+ E  CTG GADTS RVPW+ +     +  FT++++ID +
Sbjct: 276 PIGWDAWKAKGQEGHITFVEFGCTGVGADTSKRVPWLTKASEKDVLQFTNLTFIDEE 329

[Close]

BLAST of CSPI03G25040.1 vs. Swiss-Prot
Match: PME52_ARATH (Putative pectinesterase 52 OS=Arabidopsis thaliana GN=PME52 PE=2 SV=2)

HSP 1 Score: 238.4 bits (607), Expect = 1.3e-61
Identity = 115/243 (47.33%), Postives = 153/243 (62.96%), Query Frame = 1


Query: 100 GSGLQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARGSVMRRE----DIKPA 159
           G G +VT I +N H  T  S TFT+   ++VV+ ++  NTYN   S+ +      DIKPA
Sbjct: 42  GEGQRVTTITYNGHAATDVSSTFTSYPSHIVVRNLSIMNTYNRLTSLTKANGMSWDIKPA 101

Query: 160 LAALIQGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINI 219
           +A  + GDK  F+ C F+GLQDT+WD  GRH F  CYIEG ID I G GQS+Y++C IN 
Sbjct: 102 VAISVYGDKSAFYNCDFLGLQDTVWDNLGRHHFKNCYIEGAIDFIFGSGQSVYEDCHINA 161

Query: 220 PVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFL 279
              A A  ++ G+ITAQG+ + ++ SGFVFLR  V GS +V+LGRAY PFS VIF    L
Sbjct: 162 TAGALASKVSFGYITAQGRSSDSDPSGFVFLRGSVSGSTSVYLGRAYGPFSRVIFIQTDL 221

Query: 280 PSCINPAGWNSWLQAGHESDLTYSETRCTGPGADTSSRVPWVNRLDAFHIRSFTDISYID 339
            S ++P GW SW   G+E   TY+E  C G G+D S RVPW+++L +F+ +    IS   
Sbjct: 222 SSVVHPEGWYSWHYGGYEMSFTYAEVECKGAGSDMSRRVPWIDKLHSFYTKQQFSISNFI 281

[Close]

BLAST of CSPI03G25040.1 vs. TrEMBL
Match: A0A0A0LB12_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_3G536660 PE=4 SV=1)

HSP 1 Score: 697.6 bits (1799), Expect = 8.4e-198
Identity = 333/337 (98.81%), Postives = 334/337 (99.11%), Query Frame = 1


Query: 1   MEHYYSKEISLLIITTLVVLFCVKVSKALDDCELNYNDPHKVAYTIVVDKLGKGNFNTIQ 60
           MEHYYSKEISL IITTLVVLFCVKVSKALDDCELNYNDPHKVAYTIVVDKLGKGNF TIQ
Sbjct: 1   MEHYYSKEISLFIITTLVVLFCVKVSKALDDCELNYNDPHKVAYTIVVDKLGKGNFTTIQ 60

Query: 61  SAINSVPSQSTKWIRIQVSPGIYTEKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAASP 120
           SAINSVPSQSTKWIRIQ+SPGIYTEKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAASP
Sbjct: 61  SAINSVPSQSTKWIRIQISPGIYTEKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAASP 120

Query: 121 TFTASAQNLVVQGITFRNTYNARGSVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTLW 180
           TFTASAQNLVVQGITFRNTYNARGSVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTLW
Sbjct: 121 TFTASAQNLVVQGITFRNTYNARGSVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTLW 180

Query: 181 DGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAYAPLLNEGFITAQGKENPNES 240
           DGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAYAPLLNEGFITAQGKENPNES
Sbjct: 181 DGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAYAPLLNEGFITAQGKENPNES 240

Query: 241 SGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCINPAGWNSWLQAGHESDLTYSE 300
           SGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCINPAGWNSWLQAGHESDLTYSE
Sbjct: 241 SGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCINPAGWNSWLQAGHESDLTYSE 300

Query: 301 TRCTGPGADTSSRVPWVNRLDAFHIRSFTDISYIDPQ 338
           TRC GPGADTSSRVPWVNRLDAFHIRSFTDISYIDPQ
Sbjct: 301 TRCIGPGADTSSRVPWVNRLDAFHIRSFTDISYIDPQ 337

[Close]

BLAST of CSPI03G25040.1 vs. TrEMBL
Match: A0A067HG98_CITSI (Pectinesterase OS=Citrus sinensis GN=CISIN_1g043974mg PE=4 SV=1)

HSP 1 Score: 344.0 bits (881), Expect = 2.4e-91
Identity = 170/329 (51.67%), Postives = 214/329 (65.05%), Query Frame = 1


Query: 10  SLLIITTLVVLFCVKVSKALDDCELNYNDPHKVAYTIVVDKLGKGNFNTIQSAINSVPSQ 69
           S L   TL++L    VS ALD C+LN  +P KVAYTI V + G GNF  IQ AIN +P  
Sbjct: 6   SFLFAVTLLLLSS-NVSTALD-CKLNNANPLKVAYTISVSRSGGGNFTKIQDAINFIPVN 65

Query: 70  STKWIRIQVSPGIYTEKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAASPTFTASAQNL 129
           + +WIRIQ+SP IY EKVTIP  K CIFL+G+  + T+I W DH+TT +SPTFT+ ++N+
Sbjct: 66  NDQWIRIQISPEIYQEKVTIPQNKPCIFLEGASSRTTKIQWGDHDTTISSPTFTSLSENV 125

Query: 130 VVQGITFRNTYNARGSVMR---REDIKPALAALIQGDKVIFHKCGFIGLQDTLWDGPGRH 189
           V +GI F+NTYN   S  +   R ++K ALAA I GDK  F+ CGF GLQDTL+D  GRH
Sbjct: 126 VAKGILFQNTYNVPPSYRQNQERMEVKQALAARISGDKSAFYNCGFAGLQDTLFDDHGRH 185

Query: 190 LFTQCYIEGVIDVISGFGQSIYKECVINIPVNAYAPLLNEGFITAQGKENPNESSGFVFL 249
            F QCYIEG ID I G GQSIY  C +N+ +  Y P  + G+ITAQG+ + ++ SGFVF 
Sbjct: 186 YFHQCYIEGAIDFIFGNGQSIYHNCAVNVTLKEYLPEKDYGYITAQGRNSTDDPSGFVFT 245

Query: 250 RCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCINPAGWNSWLQAGHESDLTYSETRCTGP 309
            C   GSG  +LGRAY  FS VI     L   + P GWN+W    HE ++ Y+E RC G 
Sbjct: 246 SCQFLGSGKAYLGRAYGAFSRVIIVNSVLTDMVEPQGWNAWNHVEHEENIEYAEARCRGA 305

Query: 310 GADTSSRVPWVNRLDAFHIRSFTDISYID 336
           GADTS RV W   L    ++ FTD S+ID
Sbjct: 306 GADTSKRVSWEKHLSVDEVKKFTDSSFID 332

[Close]

BLAST of CSPI03G25040.1 vs. TrEMBL
Match: A0A061DSC1_THECC (Pectinesterase OS=Theobroma cacao GN=TCM_004838 PE=4 SV=1)

HSP 1 Score: 343.6 bits (880), Expect = 3.1e-91
Identity = 160/326 (49.08%), Postives = 216/326 (66.26%), Query Frame = 1


Query: 12  LIITTLVVLFCVKVSKALDDCELNYNDPHKVAYTIVVDKLGKGNFNTIQSAINSVPSQST 71
           ++    +VL    +SKAL+ C+LN  +  KVA+TI VD+ GKG+F TIQ+AI+S+PS + 
Sbjct: 23  ILALIFIVLLYFSISKALE-CQLNNANKFKVAHTIRVDQSGKGDFKTIQNAIDSIPSNND 82

Query: 72  KWIRIQVSPGIYTEKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAASPTFTASAQNLVV 131
           KWIRI +SPG + EKVTIP+ K CIFL+G+G ++T I W DH  T  S TFT+S  N+V 
Sbjct: 83  KWIRILISPGHFREKVTIPANKPCIFLEGAGSKLTSIEWGDHMDTCDSATFTSSPDNIVA 142

Query: 132 QGITFRNTYNARGSVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTLWDGPGRHLFTQC 191
           +GI+F+N YN       ++ ++PALA  I GDK  F+ C F GLQDTLWD  GRH F  C
Sbjct: 143 KGISFKNIYNIPSGPDAKKKVEPALAVRIYGDKSAFYNCAFFGLQDTLWDVQGRHYFYNC 202

Query: 192 YIEGVIDVISGFGQSIYKECVINIPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQ 251
           YIEG +D I G GQSIY++C IN+    YAP    G+ITAQG+ + ++ SGFVF  C+  
Sbjct: 203 YIEGAVDFIFGSGQSIYEKCEINLTAGKYAPEYPNGYITAQGRNSSDDPSGFVFKYCVFT 262

Query: 252 GSGNVFLGRAYRPFSTVIFHLCFLPSCINPAGWNSWLQAGHESDLTYSETRCTGPGADTS 311
           G G  +LGRAY  +S VI +   L   I  +GW++W    HE +L Y E  C GPGA+TS
Sbjct: 263 GYGKTYLGRAYGAYSRVIIYKSVLSDAILASGWDAWRYVHHEGNLIYVEADCKGPGANTS 322

Query: 312 SRVPWVNRLDAFHIRSFTDISYIDPQ 338
            RVPW+ +L A  ++ F ++SYID +
Sbjct: 323 KRVPWLKKLGASQLKKFLNLSYIDKE 347

[Close]

BLAST of CSPI03G25040.1 vs. TrEMBL
Match: V4VWS4_9ROSI (Pectinesterase (Fragment) OS=Citrus clementina GN=CICLE_v10024124mg PE=4 SV=1)

HSP 1 Score: 342.0 bits (876), Expect = 9.0e-91
Identity = 161/308 (52.27%), Postives = 203/308 (65.91%), Query Frame = 1


Query: 31  DCELNYNDPHKVAYTIVVDKLGKGNFNTIQSAINSVPSQSTKWIRIQVSPGIYTEKVTIP 90
           DC+LN  +P KVAYTI V + G GNF  IQ AIN +P  + +WIRIQ+SP IY EKVTIP
Sbjct: 2   DCKLNNANPLKVAYTISVSRSGGGNFTKIQDAINFIPVNNDQWIRIQISPEIYQEKVTIP 61

Query: 91  SEKSCIFLDGSGLQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARGSVMR-- 150
             K CIFL+G+  + T+I W DH+TT +SPTFT+ ++N+V +GI F+NTYN   S  +  
Sbjct: 62  QNKPCIFLEGASSRTTKIQWGDHDTTISSPTFTSLSENVVAKGILFQNTYNVPPSYRQNQ 121

Query: 151 -REDIKPALAALIQGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSI 210
            R ++K ALAA I GDK  F+ CGF GLQDTL+D  GRH F QCYIEG ID I G GQSI
Sbjct: 122 ERMEVKQALAARISGDKSAFYNCGFAGLQDTLFDDHGRHYFHQCYIEGAIDFIFGNGQSI 181

Query: 211 YKECVINIPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFST 270
           Y  C +N+ +  Y P  + G+ITAQG+ + ++ SGFVF  C   GSG  +LGRAY  FS 
Sbjct: 182 YHNCAVNVTLKEYLPEKDYGYITAQGRNSTDDPSGFVFTSCQFLGSGKAYLGRAYGAFSR 241

Query: 271 VIFHLCFLPSCINPAGWNSWLQAGHESDLTYSETRCTGPGADTSSRVPWVNRLDAFHIRS 330
           VI     L   + P GWN+W    HE ++ Y+E RC G GADTS RV W   L    ++ 
Sbjct: 242 VIIVNSVLTDMVEPQGWNAWNHVEHEENIEYAEARCRGAGADTSKRVSWEKHLSVDEVKK 301

Query: 331 FTDISYID 336
           FTD S+ID
Sbjct: 302 FTDSSFID 309

[Close]

BLAST of CSPI03G25040.1 vs. TrEMBL
Match: A0A067K902_JATCU (Pectinesterase OS=Jatropha curcas GN=JCGZ_14050 PE=4 SV=1)

HSP 1 Score: 333.2 bits (853), Expect = 4.2e-88
Identity = 157/319 (49.22%), Postives = 214/319 (67.08%), Query Frame = 1


Query: 18  VVLFCVKVSKALDDCELNYNDPHKVAYTIVVDKLGKGNFNTIQSAINSVPSQSTKWIRIQ 77
           +++ C  V KALD C+LN ++  KVAYTI VDK GKGNF TIQSAI+SVP  +T WIRIQ
Sbjct: 10  ILVLCSSVCKALD-CQLNKSNSLKVAYTISVDKSGKGNFTTIQSAIDSVPDGNTHWIRIQ 69

Query: 78  VSPGIYTEKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAASPTFTASAQNLVVQGITFR 137
           +SP  Y EK+TIP +K CIFL+G+  ++T I W+DH  T  S TF++ ++NL+ +GI F+
Sbjct: 70  ISPNTYREKITIPMKKPCIFLEGADKELTSIEWDDHGVTGTSATFSSYSENLIAKGIKFK 129

Query: 138 NTYNARGSVMRRE-DIKPALAALIQGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGV 197
           NTYN    V       + A++A I+GDK  F+ C F+G+QDT WD  GRH F +CY EG 
Sbjct: 130 NTYNWNLPVKAENFSWRQAVSARIRGDKSAFYDCAFVGIQDTFWDEKGRHYFYKCYFEGA 189

Query: 198 IDVISGFGQSIYKECVINIPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNV 257
           +D I G  +SIY++C++++    Y P +  G+ITAQ KE     SGFVF  C + GSG  
Sbjct: 190 VDFIFGKAKSIYEKCLLSVNKGRYDPEI-PGYITAQKKEWAEHESGFVFKNCEINGSGKA 249

Query: 258 FLGRAYRPFSTVIFHLCFLPSCINPAGWNSWLQAGHESDLTYSETRCTGPGADTSSRVPW 317
           +LGRA+ PFS+V+ +   +   I P GWN+W   GHE++ TY E    GPGA+TS RVPW
Sbjct: 250 YLGRAWGPFSSVVIYNSTISEVIVPQGWNAWDYVGHEANFTYVEVDNRGPGAETSKRVPW 309

Query: 318 VNRLDAFHIRSFTDISYID 336
           VN+L+A  +  +  +SYID
Sbjct: 310 VNQLNASELSKYLTLSYID 326

[Close]

BLAST of CSPI03G25040.1 vs. TAIR10
Match: AT2G47280.1 (AT2G47280.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 290.8 bits (743), Expect = 1.2e-78
Identity = 138/295 (46.78%), Postives = 188/295 (63.73%), Query Frame = 1


Query: 41  KVAYTIVVDKLGKGNFNTIQSAINSVPSQSTKWIRIQVSPGIYTEKVTIPSEKSCIFLDG 100
           ++AYTI VD  G GNF T+QSAI+S+   +  WIR+    GIY EKVTIP EK  I+L G
Sbjct: 30  QIAYTITVDLNGGGNFTTVQSAIDSISPPNHNWIRVFTQNGIYREKVTIPKEKGFIYLQG 89

Query: 101 SGLQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARGSVMRREDIKPALAALI 160
            G++ T I ++DH+ T  S TFTA A ++V+ GITF+NTYN   +   + +I PA+AA +
Sbjct: 90  KGIEQTVIEYDDHQATDISATFTAFADDIVISGITFKNTYNIVPN--NKREIVPAVAARM 149

Query: 161 QGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAY 220
            GD+ +     F+GLQDTL+DG GRH + +C I G ID I G+GQS++KEC +N+ +  Y
Sbjct: 150 LGDRYVVTDSSFVGLQDTLFDGKGRHYYKRCIISGGIDFIFGYGQSLFKECTLNMTLGIY 209

Query: 221 APLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCIN 280
           AP    G ITA  + +P++  GFVF  C V G G   LGRA+   + VIF    L   + 
Sbjct: 210 APDNPYGTITAHQRPSPSDEGGFVFSDCTVTGVGKTLLGRAWGSNARVIFDRSRLSDVVL 269

Query: 281 PAGWNSWLQAGHESDLTYSETRCTGPGADTSSRVPWVNRLDAFHIRSFTDISYID 336
           P GW++W   G+E DLT+ E  CTG GADTS RVPW+ +L    +  F  +S+ID
Sbjct: 270 PIGWDAWRAKGNERDLTFVEAGCTGAGADTSQRVPWLKKLSLSEVDGFASVSFID 322

[Close]

BLAST of CSPI03G25040.1 vs. TAIR10
Match: AT5G18990.1 (AT5G18990.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 290.8 bits (743), Expect = 1.2e-78
Identity = 138/302 (45.70%), Postives = 192/302 (63.58%), Query Frame = 1


Query: 39  PHKVA---YTIVVDKLGKGNFNTIQSAINSVPSQSTKWIRIQVSPGIYTEKVTIPSEKSC 98
           PH +    + ++VD+ G GNF TIQ AI+SVP  +T W  I V  G+Y EK+TIP +K  
Sbjct: 19  PHLIEAKPFEVIVDQSGHGNFTTIQKAIDSVPINNTHWFFINVKAGLYREKITIPQKKPF 78

Query: 99  IFLDGSGLQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYN--ARGSVMRREDIK 158
           I + G+G + T + W+DH + A SPTF   A N VV+ ITF N+YN  + G + +   + 
Sbjct: 79  IVIVGAGKRSTRVEWDDHASLAQSPTFATLADNTVVKKITFANSYNFPSNGKINKNPRV- 138

Query: 159 PALAALIQGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIYKECVI 218
           PA+AA I GDK  F+  GF G+QDTLWD  GRH F +C I+G +D I G GQSIY+ CVI
Sbjct: 139 PAVAAFIGGDKSAFYSVGFAGIQDTLWDSDGRHYFHRCTIQGAVDFILGSGQSIYQSCVI 198

Query: 219 NIPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLC 278
            +      P +  G+ITAQG+ N N+++GFVF+ C+V G G  +LGRA+RP+S VIF+  
Sbjct: 199 QVLGGQLGPGVT-GYITAQGRTNANDANGFVFINCLVHGFGKAYLGRAWRPYSRVIFYNS 258

Query: 279 FLPSCINPAGWNSWLQAGHESDLTYSETRCTGPGADTSSRVPWVNRLDAFHIRSFTDISY 336
            L   ++P GW  W   G+E  LTY+E  C G G++TS R  WV +L A  ++   D+S+
Sbjct: 259 NLTDVVDPLGWWEWNYQGYEKQLTYAEHGCFGSGSNTSRRAKWVKKLSASAVQHLADLSF 318

[Close]

BLAST of CSPI03G25040.1 vs. TAIR10
Match: AT3G24130.1 (AT3G24130.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 281.6 bits (719), Expect = 7.3e-76
Identity = 136/292 (46.58%), Postives = 187/292 (64.04%), Query Frame = 1


Query: 46  IVVDKLGKGNFNTIQSAINSVPSQSTKWIRIQVSPGIYTEKVTIPSEKSCIFLDGSGLQV 105
           + VD+ G GNF TIQ AI+SVP  +  W  I V  G+Y EK+ IP EK  I L G+G ++
Sbjct: 34  VFVDQSGHGNFTTIQKAIDSVPINNRHWFFINVKAGLYREKIKIPYEKPFIVLVGAGKRL 93

Query: 106 TEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYN--ARGSVMRREDIKPALAALIQGD 165
           T + W+DH + A SPTF+  A N VV+ ITF N+YN  ++G  M +    PA+AALI GD
Sbjct: 94  TRVEWDDHYSVAQSPTFSTLADNTVVKSITFANSYNFPSKGK-MNKNPRTPAVAALIGGD 153

Query: 166 KVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAYAPL 225
           K  F+  GF G+QDTLWD  GRH F +C I+G +D I G GQSIY+ CVI +      P 
Sbjct: 154 KSAFYSVGFAGIQDTLWDFDGRHYFHRCTIQGAVDFIFGTGQSIYQSCVIQVLGGQLEPG 213

Query: 226 LNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCINPAG 285
           L  G+ITAQG+ NP +++GF+F+ C+V G+G  FLGR +R +S VIF+   L   + P G
Sbjct: 214 L-AGYITAQGRTNPYDANGFIFINCLVYGTGMAFLGRPWRGYSRVIFYNSNLTDVVVPEG 273

Query: 286 WNSWLQAGHESDLTYSETRCTGPGADTSSRVPWVNRLDAFHIRSFTDISYID 336
           W++W   GHE+ L ++E  C G GA+   RV WV +L    I++  D+S+I+
Sbjct: 274 WDAWNFVGHENQLVFAEHGCFGSGANIGRRVKWVKKLSESAIQNLADLSFIN 323

[Close]

BLAST of CSPI03G25040.1 vs. TAIR10
Match: AT2G19150.1 (AT2G19150.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 270.0 bits (689), Expect = 2.2e-72
Identity = 130/297 (43.77%), Postives = 181/297 (60.94%), Query Frame = 1


Query: 41  KVAYTIVVDKLGKGNFNTIQSAINSVPSQSTKWIRIQVSPGIYTEKVTIPSEKSCIFLDG 100
           ++A TI+V+      F T+QSAI+S+P Q+  WIRI +S GIY+EKVTIP  K  I++ G
Sbjct: 36  QIAKTIIVNPNDARYFKTVQSAIDSIPLQNQDWIRILISNGIYSEKVTIPRGKGYIYMQG 95

Query: 101 SGLQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARGSVMRREDIKPALAALI 160
            G++ T I + DH+ T  S TFT+   N+++ GITF+N YN   S       KPA+AA++
Sbjct: 96  GGIEKTIIAYGDHQLTNTSATFTSYPSNIIITGITFKNKYNIASS---SSPTKPAVAAMM 155

Query: 161 QGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAY 220
            GDK       F G QDTL+D  GRH + +C I G ID I G  QSI++ C + + V  Y
Sbjct: 156 LGDKYAIIDSSFDGFQDTLYDDYGRHYYKRCVISGGIDFIFGGAQSIFEGCTLKLRVGIY 215

Query: 221 APLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCIN 280
            P    G ITAQG+++P +  GFVF  C V GSG   LGRA++ +S VIF+       I 
Sbjct: 216 PPNEVYGTITAQGRDSPTDKGGFVFKDCTVMGSGKALLGRAWKSYSRVIFYRSMFSDNIL 275

Query: 281 PAGWNSWLQAGHESDLTYSETRCTGPGADTSSRVPWVNRLDAFHIRSFTDISYIDPQ 338
           P GW++W   G E  +T+ E  CTG GADTS RVPW+ +     +  FT++++ID +
Sbjct: 276 PIGWDAWKAKGQEGHITFVEFGCTGVGADTSKRVPWLTKASEKDVLQFTNLTFIDEE 329

[Close]

BLAST of CSPI03G25040.1 vs. TAIR10
Match: AT5G26810.1 (AT5G26810.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 238.4 bits (607), Expect = 7.1e-63
Identity = 115/243 (47.33%), Postives = 153/243 (62.96%), Query Frame = 1


Query: 100 GSGLQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARGSVMRRE----DIKPA 159
           G G +VT I +N H  T  S TFT+   ++VV+ ++  NTYN   S+ +      DIKPA
Sbjct: 42  GEGQRVTTITYNGHAATDVSSTFTSYPSHIVVRNLSIMNTYNRLTSLTKANGMSWDIKPA 101

Query: 160 LAALIQGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINI 219
           +A  + GDK  F+ C F+GLQDT+WD  GRH F  CYIEG ID I G GQS+Y++C IN 
Sbjct: 102 VAISVYGDKSAFYNCDFLGLQDTVWDNLGRHHFKNCYIEGAIDFIFGSGQSVYEDCHINA 161

Query: 220 PVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFL 279
              A A  ++ G+ITAQG+ + ++ SGFVFLR  V GS +V+LGRAY PFS VIF    L
Sbjct: 162 TAGALASKVSFGYITAQGRSSDSDPSGFVFLRGSVSGSTSVYLGRAYGPFSRVIFIQTDL 221

Query: 280 PSCINPAGWNSWLQAGHESDLTYSETRCTGPGADTSSRVPWVNRLDAFHIRSFTDISYID 339
            S ++P GW SW   G+E   TY+E  C G G+D S RVPW+++L +F+ +    IS   
Sbjct: 222 SSVVHPEGWYSWHYGGYEMSFTYAEVECKGAGSDMSRRVPWIDKLHSFYTKQQFSISNFI 281

[Close]

BLAST of CSPI03G25040.1 vs. NCBI nr
Match: gi|700203001|gb|KGN58134.1| (hypothetical protein Csa_3G536660 [Cucumis sativus])

HSP 1 Score: 697.6 bits (1799), Expect = 1.2e-197
Identity = 333/337 (98.81%), Postives = 334/337 (99.11%), Query Frame = 1


Query: 1   MEHYYSKEISLLIITTLVVLFCVKVSKALDDCELNYNDPHKVAYTIVVDKLGKGNFNTIQ 60
           MEHYYSKEISL IITTLVVLFCVKVSKALDDCELNYNDPHKVAYTIVVDKLGKGNF TIQ
Sbjct: 1   MEHYYSKEISLFIITTLVVLFCVKVSKALDDCELNYNDPHKVAYTIVVDKLGKGNFTTIQ 60

Query: 61  SAINSVPSQSTKWIRIQVSPGIYTEKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAASP 120
           SAINSVPSQSTKWIRIQ+SPGIYTEKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAASP
Sbjct: 61  SAINSVPSQSTKWIRIQISPGIYTEKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAASP 120

Query: 121 TFTASAQNLVVQGITFRNTYNARGSVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTLW 180
           TFTASAQNLVVQGITFRNTYNARGSVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTLW
Sbjct: 121 TFTASAQNLVVQGITFRNTYNARGSVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTLW 180

Query: 181 DGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAYAPLLNEGFITAQGKENPNES 240
           DGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAYAPLLNEGFITAQGKENPNES
Sbjct: 181 DGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAYAPLLNEGFITAQGKENPNES 240

Query: 241 SGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCINPAGWNSWLQAGHESDLTYSE 300
           SGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCINPAGWNSWLQAGHESDLTYSE
Sbjct: 241 SGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCINPAGWNSWLQAGHESDLTYSE 300

Query: 301 TRCTGPGADTSSRVPWVNRLDAFHIRSFTDISYIDPQ 338
           TRC GPGADTSSRVPWVNRLDAFHIRSFTDISYIDPQ
Sbjct: 301 TRCIGPGADTSSRVPWVNRLDAFHIRSFTDISYIDPQ 337

[Close]

BLAST of CSPI03G25040.1 vs. NCBI nr
Match: gi|778688667|ref|XP_011652807.1| (PREDICTED: probable pectinesterase 55 [Cucumis sativus])

HSP 1 Score: 536.2 bits (1380), Expect = 4.6e-149
Identity = 252/253 (99.60%), Postives = 252/253 (99.60%), Query Frame = 1


Query: 85  EKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARG 144
           EKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARG
Sbjct: 33  EKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARG 92

Query: 145 SVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFG 204
           SVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFG
Sbjct: 93  SVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFG 152

Query: 205 QSIYKECVINIPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRP 264
           QSIYKECVINIPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRP
Sbjct: 153 QSIYKECVINIPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRP 212

Query: 265 FSTVIFHLCFLPSCINPAGWNSWLQAGHESDLTYSETRCTGPGADTSSRVPWVNRLDAFH 324
           FSTVIFHLCFLPSCINPAGWNSWLQAGHESDLTYSETRC GPGADTSSRVPWVNRLDAFH
Sbjct: 213 FSTVIFHLCFLPSCINPAGWNSWLQAGHESDLTYSETRCIGPGADTSSRVPWVNRLDAFH 272

Query: 325 IRSFTDISYIDPQ 338
           IRSFTDISYIDPQ
Sbjct: 273 IRSFTDISYIDPQ 285

[Close]

BLAST of CSPI03G25040.1 vs. NCBI nr
Match: gi|659093698|ref|XP_008447669.1| (PREDICTED: putative pectinesterase 52 [Cucumis melo])

HSP 1 Score: 479.2 bits (1232), Expect = 6.7e-132
Identity = 220/249 (88.35%), Postives = 232/249 (93.17%), Query Frame = 1


Query: 89  IPSEKSCIFLDGSGLQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARGSVMR 148
           IP +KSCIFLDGSG +VTEI WNDH TTA SPTFT+ AQNLVVQGITFRNTYNA GSV R
Sbjct: 2   IPPDKSCIFLDGSGFRVTEIQWNDHATTATSPTFTSYAQNLVVQGITFRNTYNALGSVTR 61

Query: 149 REDIKPALAALIQGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIY 208
           REDIKPA+AALIQGDK IFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIY
Sbjct: 62  REDIKPAIAALIQGDKAIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIY 121

Query: 209 KECVINIPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTV 268
           KECVINIP+NAYAPLLNEGFITAQGKENP+ESSGFVF+RCIV+GSG VFLGRAYRPFSTV
Sbjct: 122 KECVINIPINAYAPLLNEGFITAQGKENPSESSGFVFIRCIVKGSGKVFLGRAYRPFSTV 181

Query: 269 IFHLCFLPSCINPAGWNSWLQAGHESDLTYSETRCTGPGADTSSRVPWVNRLDAFHIRSF 328
           IFHLCFLPSCI+PAGWNSW Q GHES+LTYSETRC GPGADTSSRVPW+ RLD + IR F
Sbjct: 182 IFHLCFLPSCIDPAGWNSWQQVGHESNLTYSETRCIGPGADTSSRVPWLKRLDVYQIRRF 241

Query: 329 TDISYIDPQ 338
           TDISYIDPQ
Sbjct: 242 TDISYIDPQ 250

[Close]

BLAST of CSPI03G25040.1 vs. NCBI nr
Match: gi|985469142|ref|XP_015380662.1| (PREDICTED: probable pectinesterase 55 [Citrus sinensis])

HSP 1 Score: 344.0 bits (881), Expect = 3.4e-91
Identity = 170/329 (51.67%), Postives = 214/329 (65.05%), Query Frame = 1


Query: 10  SLLIITTLVVLFCVKVSKALDDCELNYNDPHKVAYTIVVDKLGKGNFNTIQSAINSVPSQ 69
           S L   TL++L    VS ALD C+LN  +P KVAYTI V + G GNF  IQ AIN +P  
Sbjct: 6   SFLFAVTLLLLSS-NVSTALD-CKLNNANPLKVAYTISVSRSGGGNFTKIQDAINFIPVN 65

Query: 70  STKWIRIQVSPGIYTEKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAASPTFTASAQNL 129
           + +WIRIQ+SP IY EKVTIP  K CIFL+G+  + T+I W DH+TT +SPTFT+ ++N+
Sbjct: 66  NDQWIRIQISPEIYQEKVTIPQNKPCIFLEGASSRTTKIQWGDHDTTISSPTFTSLSENV 125

Query: 130 VVQGITFRNTYNARGSVMR---REDIKPALAALIQGDKVIFHKCGFIGLQDTLWDGPGRH 189
           V +GI F+NTYN   S  +   R ++K ALAA I GDK  F+ CGF GLQDTL+D  GRH
Sbjct: 126 VAKGILFQNTYNVPPSYRQNQERMEVKQALAARISGDKSAFYNCGFAGLQDTLFDDHGRH 185

Query: 190 LFTQCYIEGVIDVISGFGQSIYKECVINIPVNAYAPLLNEGFITAQGKENPNESSGFVFL 249
            F QCYIEG ID I G GQSIY  C +N+ +  Y P  + G+ITAQG+ + ++ SGFVF 
Sbjct: 186 YFHQCYIEGAIDFIFGNGQSIYHNCAVNVTLKEYLPEKDYGYITAQGRNSTDDPSGFVFT 245

Query: 250 RCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCINPAGWNSWLQAGHESDLTYSETRCTGP 309
            C   GSG  +LGRAY  FS VI     L   + P GWN+W    HE ++ Y+E RC G 
Sbjct: 246 SCQFLGSGKAYLGRAYGAFSRVIIVNSVLTDMVEPQGWNAWNHVEHEENIEYAEARCRGA 305

Query: 310 GADTSSRVPWVNRLDAFHIRSFTDISYID 336
           GADTS RV W   L    ++ FTD S+ID
Sbjct: 306 GADTSKRVSWEKHLSVDEVKKFTDSSFID 332

[Close]

BLAST of CSPI03G25040.1 vs. NCBI nr
Match: gi|590719762|ref|XP_007051145.1| (Pectinesterase, putative [Theobroma cacao])

HSP 1 Score: 343.6 bits (880), Expect = 4.4e-91
Identity = 160/326 (49.08%), Postives = 216/326 (66.26%), Query Frame = 1


Query: 12  LIITTLVVLFCVKVSKALDDCELNYNDPHKVAYTIVVDKLGKGNFNTIQSAINSVPSQST 71
           ++    +VL    +SKAL+ C+LN  +  KVA+TI VD+ GKG+F TIQ+AI+S+PS + 
Sbjct: 23  ILALIFIVLLYFSISKALE-CQLNNANKFKVAHTIRVDQSGKGDFKTIQNAIDSIPSNND 82

Query: 72  KWIRIQVSPGIYTEKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAASPTFTASAQNLVV 131
           KWIRI +SPG + EKVTIP+ K CIFL+G+G ++T I W DH  T  S TFT+S  N+V 
Sbjct: 83  KWIRILISPGHFREKVTIPANKPCIFLEGAGSKLTSIEWGDHMDTCDSATFTSSPDNIVA 142

Query: 132 QGITFRNTYNARGSVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTLWDGPGRHLFTQC 191
           +GI+F+N YN       ++ ++PALA  I GDK  F+ C F GLQDTLWD  GRH F  C
Sbjct: 143 KGISFKNIYNIPSGPDAKKKVEPALAVRIYGDKSAFYNCAFFGLQDTLWDVQGRHYFYNC 202

Query: 192 YIEGVIDVISGFGQSIYKECVINIPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQ 251
           YIEG +D I G GQSIY++C IN+    YAP    G+ITAQG+ + ++ SGFVF  C+  
Sbjct: 203 YIEGAVDFIFGSGQSIYEKCEINLTAGKYAPEYPNGYITAQGRNSSDDPSGFVFKYCVFT 262

Query: 252 GSGNVFLGRAYRPFSTVIFHLCFLPSCINPAGWNSWLQAGHESDLTYSETRCTGPGADTS 311
           G G  +LGRAY  +S VI +   L   I  +GW++W    HE +L Y E  C GPGA+TS
Sbjct: 263 GYGKTYLGRAYGAYSRVIIYKSVLSDAILASGWDAWRYVHHEGNLIYVEADCKGPGANTS 322

Query: 312 SRVPWVNRLDAFHIRSFTDISYIDPQ 338
            RVPW+ +L A  ++ F ++SYID +
Sbjct: 323 KRVPWLKKLGASQLKKFLNLSYIDKE 347

The following BLAST results are available for this feature:

BLAST of CSPI03G25040.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
PME66_ARATH	2.1e-77	46.78	Probable pectinesterase 66 OS=Arabidopsis thaliana GN=PME66 PE=2 SV=2	[more]
PME55_ARATH	2.1e-77	45.70	Probable pectinesterase 55 OS=Arabidopsis thaliana GN=PME55 PE=2 SV=1	[more]
PME29_ARATH	1.3e-74	46.58	Probable pectinesterase 29 OS=Arabidopsis thaliana GN=PME29 PE=2 SV=1	[more]
PME10_ARATH	3.9e-71	43.77	Putative pectinesterase 10 OS=Arabidopsis thaliana GN=PME10 PE=2 SV=1	[more]
PME52_ARATH	1.3e-61	47.33	Putative pectinesterase 52 OS=Arabidopsis thaliana GN=PME52 PE=2 SV=2	[more]

BLAST of CSPI03G25040.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LB12_CUCSA	8.4e-198	98.81	Pectinesterase OS=Cucumis sativus GN=Csa_3G536660 PE=4 SV=1	[more]
A0A067HG98_CITSI	2.4e-91	51.67	Pectinesterase OS=Citrus sinensis GN=CISIN_1g043974mg PE=4 SV=1	[more]
A0A061DSC1_THECC	3.1e-91	49.08	Pectinesterase OS=Theobroma cacao GN=TCM_004838 PE=4 SV=1	[more]
V4VWS4_9ROSI	9.0e-91	52.27	Pectinesterase (Fragment) OS=Citrus clementina GN=CICLE_v10024124mg PE=4 SV=1	[more]
A0A067K902_JATCU	4.2e-88	49.22	Pectinesterase OS=Jatropha curcas GN=JCGZ_14050 PE=4 SV=1	[more]

BLAST of CSPI03G25040.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G47280.1	1.2e-78	46.78	Pectin lyase-like superfamily protein	[more]
AT5G18990.1	1.2e-78	45.70	Pectin lyase-like superfamily protein	[more]
AT3G24130.1	7.3e-76	46.58	Pectin lyase-like superfamily protein	[more]
AT2G19150.1	2.2e-72	43.77	Pectin lyase-like superfamily protein	[more]
AT5G26810.1	7.1e-63	47.33	Pectin lyase-like superfamily protein	[more]

BLAST of CSPI03G25040.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700203001\|gb\|KGN58134.1\|	1.2e-197	98.81	hypothetical protein Csa_3G536660 [Cucumis sativus]	[more]
gi\|778688667\|ref\|XP_011652807.1\|	4.6e-149	99.60	PREDICTED: probable pectinesterase 55 [Cucumis sativus]	[more]
gi\|659093698\|ref\|XP_008447669.1\|	6.7e-132	88.35	PREDICTED: putative pectinesterase 52 [Cucumis melo]	[more]
gi\|985469142\|ref\|XP_015380662.1\|	3.4e-91	51.67	PREDICTED: probable pectinesterase 55 [Citrus sinensis]	[more]
gi\|590719762\|ref\|XP_007051145.1\|	4.4e-91	49.08	Pectinesterase, putative [Theobroma cacao]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR000070	Pectinesterase_cat
IPR011050	Pectin_lyase_fold/virulence
IPR012334	Pectin_lyas_fold

Vocabulary: **Cellular Component**
Term	Definition
GO:0005618	cell wall

Vocabulary: **Molecular Function**
Term	Definition
GO:0030599	pectinesterase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0042545	cell wall modification

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0005982	starch metabolic process
biological_process	GO:0005985	sucrose metabolic process
cellular_component	GO:0005618	cell wall
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0045330	aspartyl esterase activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CSPI03G25040	CSPI03G25040	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CSPI03G25040.1	CSPI03G25040.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI03G25040.1.cds1	CSPI03G25040.1.cds1	CDS
CSPI03G25040.1.cds2	CSPI03G25040.1.cds2	CDS
CSPI03G25040.1.cds3	CSPI03G25040.1.cds3	CDS
CSPI03G25040.1.cds4	CSPI03G25040.1.cds4	CDS
CSPI03G25040.1.cds5	CSPI03G25040.1.cds5	CDS
CSPI03G25040.1.cds6	CSPI03G25040.1.cds6	CDS

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 46..334 score: 1.5
IPR011050	Pectin lyase fold/virulence factor	unknown	SSF51126	Pectin lyase-like	coord: 42..336 score: 6.7
IPR012334	Pectin lyase fold	GENE3D	G3DSA:2.160.20.10		coord: 42..337 score: 1.2
None	No IPR available	PANTHER	PTHR31321	FAMILY NOT NAMED	coord: 11..335 score: 4.6E
None	No IPR available	PANTHER	PTHR31321:SF32	PECTINESTERASE 52-RELATED	coord: 11..335 score: 4.6E