CSPI02G20460.1 (mRNA) Wild cucumber (PI 183967)

NameCSPI02G20460.1
TypemRNA
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionElongation factor 2
LocationChr2 : 18127628 .. 18131131 (-)
Sequence length1074
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAACCCTAGGTGGCGCAAAAGGAAATGCTTCATTGAGACCGCATATTCAGCCTCACTATTACTTTTCACCCGCACTCTGTTTCCTCCTCACACGCACTCTGTTTCGCTTCTTGTACTTCTCTTTTCTGGTAGCTCAAGGGAAACTCTCTCTTCCTTTGCTGTCCCTTCATCGTCTCTTAGCTTCTTCTTTCCTGTAACAACTCTCTCTCTCTACGCTTCATTTCCTTGCAATTGTGTCTATTTTCCTTCACTCGTGCTTTCATTTAGGTTTTGTGCGTTTGAGATCTGTTTTCTTAGTCTTTTATATTCGATGAGTTTGTTTTAGATCTGTTTTTGTTGTTGCTGTATTCGATATATTAAATGGTTTAATCAATTGTTGCGATGTGTACTTAGATTATGAGATTTAAGTTGACGAGTAAGCTACGAAGAAGCTTTATGTTGAAGATTATTGCTAAATTACTTTCTACTATTTGATCTGATTATTCCCTCGTTTAACAGTGATATATTAATTTTTTCTGGCAGTTCATTGTTTCTAAATCTTGGACATCAGGAAATTTGAAGACTTGTCACGATGGTTAGTGTGCTCTTCTCTTGACGATGATAATGTTTGGGATCTGAAAAGTTCTTTAATAATCTTTAATTTTTTGTGTGTTCAGGTGAAGTTTACAGCCGAGGAGCTTCGTAGGATTATGGACTATAAGCATAACATTCGTAATATGTCTGTTATTGCTCACGTCGATCATGGTAAAATTTTTAACATGGGCTGATTTTTTAGGGTTATCAATGTTGGAAGAAATAAAAATCTTTCTGTTGGACATTAATTATAAAGATGCGCACCAATTTAAGTTCACTTCCGCACTAAGTTTTTGTTAGCTTAATGATGCTTCTAGTTGAGGCAGGCCTATTTGAACTAAATTTTTTTTGCTGATATATTGGATCTTTTTGGAAAGAAACATCAAGAAATTTAACGATCAGGAAACTCATGTAGACTACTTTATGGCTATATTTGTGGTGTTCACTCATGATATCGTATGCTTGATTGTTATATACTCGCTGATCTATGAAACCGAGAGGATTTTTCTATTTTTATGGGTGACTTCCTCTTAGCTTGGCTTTCTTCTCTCTGCAATTCCATACATGTTTCCTCTAAAAAACTATTGCTTGAGCTTATATTTTCTCATTGATCTAAATCATTCGTATTGACAGTAAAAAGAACATTTTATGTTTCTTACGAACTAATTTTAAATTGTTACTATGCATCTATTTTAACCCTTATTTGATCACCCCCTTTTTCCTTCTATTTCAGGAAAGTCGACACTTACAGATTCTCTTGTGGCTGCCGCGGGTATCATTGCACAAGAAGTTGCCGGTGATGTACGAATGACAGATACTCGCCAAGATGAGGCAGAGCGTGGTATCACCATTAAATCTACTGGAATCTCCCTCTACTATGAGATGTCTGATGAATCCTTGAAAAGTTACAAGGGAGAGAGACATGGAAATGAGTATCTTATCAATCTTATTGATTCACCTGGGCACGTTGACTTCTCGTCGGAGGTTACAGCTGCTTTACGTATTACTGATGGTGCCCTTGTTGTGGTGGATTGCATCGAAGGTGTTTGTGTCCAAACAGAGACTGTGCTCCGTCAGGCTTTGGGAGAGAGGATTAGACCTGTCTTGACTGTTAACAAGATGGATAGGTGCTTCCTTGAACTTCAAGTGGATGGAGAAGAGGCTTATCAAACATTCCAGAGGGTCATTGAGAATGCCAATGTGATTATGGCCACGTATGAGGATCCACTTCTGGGCGATGTTCAAGTGTACCCGGAGAAAGGAACAGTTGCTTTCTCTGCTGGTTTGCACGGCTGGGCATTTACCCTGACTAACTTTGCTAAGATGTATGCCTCCAAGTTTGGAGTGGATGAGGCGAAGATGATGGAGAGACTTTGGGGTGAGAATTTCTTTGATCCTGCAACCAAGAAGTGGACAAGCAAGAACACTGGCACGGCAACATGCAAGCGTGGGTTTGTCCAGTTCTGCTACGAACCCATCAAGCAGATCATTGCAACTTGCATGAACGATCGGAAGGACAAGCTATGGCCCATGTTGCAGAAGCTTGGTGTTGTCATGAAGAACTAGTTGTGGTGTTGGGGAAACATGTTGTGCAAAGAAATTCTTTGGCCTCTGTATATCATGGAAATGTTGTCCATTGAGCGGTGCAGTATGTTGCAAGGACGGTAGTAATTGTTGCCCCCTTGATTATCCCATTTGTGACAATGACAGGAACCAATGCCTCAAGGTTTGTTCTTTTGATACGGTATTTGAGATGAAAATATATTTTTACTCTCATAATTAGTCAGACCTAGCTCGTTTTGAAGATTTACAATCTTCATGTTGTTTTTCTCGCTTCAAACAAATTTCATCTTCTGAAAGTTGATTCATTTGCATAAGTGCATGTTAAAGAGAAAAAAATATATTACATGTTAGGATGTACACATTACAACTCTTGCCTTGTCCAACCCGTGTGAAGGGGGAGTTTAGGATGTTTGATATTTGTTTGGGTAATCTCTCTTCTTTCTTCTCTTAAGAAAAGTTGCCAGAGTTTCATCTATATTGTCTATTTGGTTTTTCTCTACTATTTATGAGGAGGCACTTGTATTGGACTCAAGTTGGTTTATGCTACAATATTTATTTCTTCAAACTTTTGTTAAGAAATAAATAAAAGGGCATACAAGAGAGTTTACTAACCAGTGCCCATGACGAGGCATTAAACCTAGCTCCCTCCTGTACTTTCTCACTTGATCTCTTCGTGCCTTAAAAATGTTGTTATTTGTCTCGATCTACATACTTCAAATAATAATAATAATTAACCTACCACAAAATTTTGCATTATGGTACTGAAAAAGCGAACTCAAGAGAACTTTAACCTGGTAGAAAATTAGTACACTTATTGAACACCCACCCATTTACAGCTAGATGTGTATGAATAAAATAACATAACTTCTAGCAAAATAACATTATTCAATAGTGCATCATTGAAGTAACTATATGTTACAAGTGTTTCCTTTTCTTTGTTCTCTATGTATACTTTTCATATTTTAGACTAACAGTTTATATTAATAATATGATATTATCATGACAGCGAATAATGAATGGTACAGGAATAGAAGTACTTGAGAGTAGGAGTTTTTCTGTATCTGGTACTTGGAGCTCTTTCTAAGGTTAGGATTTTGTGAAATAGCTTCATATATAAAACGAATAAAGGTTGCCATTTCTATTACCACTTTGTTACTAGCTTTTAGCTATATTTGAAGTCTAAAGAGCTTCAATTTGAGAGGCTCCTTGAGTTGCTATCACATCACATTGTAGTTTTTAAAAACTTTCTTATGTCATTATCTTTCTGCTGTTGTCCAAGAACTTTCATGGATCATCTTTGGAGGCTTCTTACCTTGATCGAGGTCGGAGTTTTATCAAGTTCTGGCTTGTTTTCTTTCGACTTCTGA

mRNA sequence

ATGGTGAAGTTTACAGCCGAGGAGCTTCGTAGGATTATGGACTATAAGCATAACATTCGTAATATGTCTGTTATTGCTCACGTCGATCATGGAAAGTCGACACTTACAGATTCTCTTGTGGCTGCCGCGGGTATCATTGCACAAGAAGTTGCCGGTGATGTACGAATGACAGATACTCGCCAAGATGAGGCAGAGCGTGGTATCACCATTAAATCTACTGGAATCTCCCTCTACTATGAGATGTCTGATGAATCCTTGAAAAGTTACAAGGGAGAGAGACATGGAAATGAGTATCTTATCAATCTTATTGATTCACCTGGGCACGTTGACTTCTCGTCGGAGGTTACAGCTGCTTTACGTATTACTGATGGTGCCCTTGTTGTGGTGGATTGCATCGAAGGTGTTTGTGTCCAAACAGAGACTGTGCTCCGTCAGGCTTTGGGAGAGAGGATTAGACCTGTCTTGACTGTTAACAAGATGGATAGGTGCTTCCTTGAACTTCAAGTGGATGGAGAAGAGGCTTATCAAACATTCCAGAGGGTCATTGAGAATGCCAATGTGATTATGGCCACGTATGAGGATCCACTTCTGGGCGATGTTCAAGTGTACCCGGAGAAAGGAACAGTTGCTTTCTCTGCTGGTTTGCACGGCTGGGCATTTACCCTGACTAACTTTGCTAAGATGTATGCCTCCAAGTTTGGAGTGGATGAGGCGAAGATGATGGAGAGACTTTGGGGTGAGAATTTCTTTGATCCTGCAACCAAGAAGTGGACAAGCAAGAACACTGGCACGGCAACATGCAAGCGTGGGTTTGTCCAGTTCTGCTACGAACCCATCAAGCAGATCATTGCAACTTGCATGAACGATCGGAAGGACAAGCTATGGCCCATGTTGCAGAAGCTTGGTGTTTATGTTGCAAGGACGGTAGTAATTGTTGCCCCCTTGATTATCCCATTTGTGACAATGACAGGAACCAATGCCTCAAGAACTTTCATGGATCATCTTTGGAGGCTTCTTACCTTGATCGAGGTCGGAGTTTTATCAAGTTCTGGCTTGTTTTCTTTCGACTTCTGA

Coding sequence (CDS)

ATGGTGAAGTTTACAGCCGAGGAGCTTCGTAGGATTATGGACTATAAGCATAACATTCGTAATATGTCTGTTATTGCTCACGTCGATCATGGAAAGTCGACACTTACAGATTCTCTTGTGGCTGCCGCGGGTATCATTGCACAAGAAGTTGCCGGTGATGTACGAATGACAGATACTCGCCAAGATGAGGCAGAGCGTGGTATCACCATTAAATCTACTGGAATCTCCCTCTACTATGAGATGTCTGATGAATCCTTGAAAAGTTACAAGGGAGAGAGACATGGAAATGAGTATCTTATCAATCTTATTGATTCACCTGGGCACGTTGACTTCTCGTCGGAGGTTACAGCTGCTTTACGTATTACTGATGGTGCCCTTGTTGTGGTGGATTGCATCGAAGGTGTTTGTGTCCAAACAGAGACTGTGCTCCGTCAGGCTTTGGGAGAGAGGATTAGACCTGTCTTGACTGTTAACAAGATGGATAGGTGCTTCCTTGAACTTCAAGTGGATGGAGAAGAGGCTTATCAAACATTCCAGAGGGTCATTGAGAATGCCAATGTGATTATGGCCACGTATGAGGATCCACTTCTGGGCGATGTTCAAGTGTACCCGGAGAAAGGAACAGTTGCTTTCTCTGCTGGTTTGCACGGCTGGGCATTTACCCTGACTAACTTTGCTAAGATGTATGCCTCCAAGTTTGGAGTGGATGAGGCGAAGATGATGGAGAGACTTTGGGGTGAGAATTTCTTTGATCCTGCAACCAAGAAGTGGACAAGCAAGAACACTGGCACGGCAACATGCAAGCGTGGGTTTGTCCAGTTCTGCTACGAACCCATCAAGCAGATCATTGCAACTTGCATGAACGATCGGAAGGACAAGCTATGGCCCATGTTGCAGAAGCTTGGTGTTTATGTTGCAAGGACGGTAGTAATTGTTGCCCCCTTGATTATCCCATTTGTGACAATGACAGGAACCAATGCCTCAAGAACTTTCATGGATCATCTTTGGAGGCTTCTTACCTTGATCGAGGTCGGAGTTTTATCAAGTTCTGGCTTGTTTTCTTTCGACTTCTGA
BLAST of CSPI02G20460.1 vs. Swiss-Prot
Match: EF2_ARATH (Elongation factor 2 OS=Arabidopsis thaliana GN=LOS1 PE=1 SV=1)

HSP 1 Score: 580.5 bits (1495), Expect = 1.3e-164
Identity = 286/303 (94.39%), Postives = 294/303 (97.03%), Query Frame = 1

Query: 1   MVKFTAEELRRIMDYKHNIRNMSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGDVRMTDTR 60
           MVKFTA+ELRRIMDYKHNIRNMSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGDVRMTDTR
Sbjct: 1   MVKFTADELRRIMDYKHNIRNMSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGDVRMTDTR 60

Query: 61  QDEAERGITIKSTGISLYYEMSDESLKSYKGERHGNEYLINLIDSPGHVDFSSEVTAALR 120
            DEAERGITIKSTGISLYYEM+DESLKS+ G R GNEYLINLIDSPGHVDFSSEVTAALR
Sbjct: 61  ADEAERGITIKSTGISLYYEMTDESLKSFTGARDGNEYLINLIDSPGHVDFSSEVTAALR 120

Query: 121 ITDGALVVVDCIEGVCVQTETVLRQALGERIRPVLTVNKMDRCFLELQVDGEEAYQTFQR 180
           ITDGALVVVDCIEGVCVQTETVLRQALGERIRPVLTVNKMDRCFLELQVDGEEAYQTF R
Sbjct: 121 ITDGALVVVDCIEGVCVQTETVLRQALGERIRPVLTVNKMDRCFLELQVDGEEAYQTFSR 180

Query: 181 VIENANVIMATYEDPLLGDVQVYPEKGTVAFSAGLHGWAFTLTNFAKMYASKFGVDEAKM 240
           VIENANVIMATYEDPLLGDVQVYPEKGTVAFSAGLHGWAFTLTNFAKMYASKFGV E+KM
Sbjct: 181 VIENANVIMATYEDPLLGDVQVYPEKGTVAFSAGLHGWAFTLTNFAKMYASKFGVVESKM 240

Query: 241 MERLWGENFFDPATKKWTSKNTGTATCKRGFVQFCYEPIKQIIATCMNDRKDKLWPMLQK 300
           MERLWGENFFDPAT+KW+ KNTG+ TCKRGFVQFCYEPIKQIIATCMND+KDKLWPML K
Sbjct: 241 MERLWGENFFDPATRKWSGKNTGSPTCKRGFVQFCYEPIKQIIATCMNDQKDKLWPMLAK 300

Query: 301 LGV 304
           LGV
Sbjct: 301 LGV 303

BLAST of CSPI02G20460.1 vs. Swiss-Prot
Match: EF2_BETVU (Elongation factor 2 OS=Beta vulgaris PE=2 SV=1)

HSP 1 Score: 563.5 bits (1451), Expect = 1.6e-159
Identity = 277/303 (91.42%), Postives = 292/303 (96.37%), Query Frame = 1

Query: 1   MVKFTAEELRRIMDYKHNIRNMSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGDVRMTDTR 60
           MVKFTA+ELR IMD KHNIRNMSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGDVRMTDTR
Sbjct: 1   MVKFTADELRAIMDCKHNIRNMSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGDVRMTDTR 60

Query: 61  QDEAERGITIKSTGISLYYEMSDESLKSYKGERHGNEYLINLIDSPGHVDFSSEVTAALR 120
            DEAERGITIKSTGISLYY+M+DE+L+SYKGER GN+YLINLIDSPGHVDFSSEVTAALR
Sbjct: 61  ADEAERGITIKSTGISLYYQMTDEALQSYKGERKGNDYLINLIDSPGHVDFSSEVTAALR 120

Query: 121 ITDGALVVVDCIEGVCVQTETVLRQALGERIRPVLTVNKMDRCFLELQVDGEEAYQTFQR 180
           ITDGALVVVDCIEGVCVQTETVLRQALGERIRPVLTVNKMDRCFLELQVDGEEAY TFQ+
Sbjct: 121 ITDGALVVVDCIEGVCVQTETVLRQALGERIRPVLTVNKMDRCFLELQVDGEEAYTTFQK 180

Query: 181 VIENANVIMATYEDPLLGDVQVYPEKGTVAFSAGLHGWAFTLTNFAKMYASKFGVDEAKM 240
           VIENANVIMATYEDPLLGDVQVYPEKGTVAFSAGLHGWAFTL+NFAKMYASKFGVDE+KM
Sbjct: 181 VIENANVIMATYEDPLLGDVQVYPEKGTVAFSAGLHGWAFTLSNFAKMYASKFGVDESKM 240

Query: 241 MERLWGENFFDPATKKWTSKNTGTATCKRGFVQFCYEPIKQIIATCMNDRKDKLWPMLQK 300
           MERLWGENFFDPATKKWT+KN+G A+CKRGFVQFCYEPIKQIIA CMND+KDKL   + K
Sbjct: 241 MERLWGENFFDPATKKWTTKNSGNASCKRGFVQFCYEPIKQIIAACMNDQKDKLLAHVTK 300

Query: 301 LGV 304
           LG+
Sbjct: 301 LGI 303

BLAST of CSPI02G20460.1 vs. Swiss-Prot
Match: EF2_PARKE (Elongation factor 2 OS=Parachlorella kessleri PE=2 SV=1)

HSP 1 Score: 482.3 bits (1240), Expect = 4.7e-135
Identity = 234/304 (76.97%), Postives = 264/304 (86.84%), Query Frame = 1

Query: 1   MVKFTAEELRRIMDYKHNIRNMSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGDVRMTDTR 60
           MVKFT +++R +M+Y++NIRNMSVIAHVDHGKSTLTDSLVAAAGIIA E AGD R+TDTR
Sbjct: 1   MVKFTIDQIRGLMEYQNNIRNMSVIAHVDHGKSTLTDSLVAAAGIIAFEQAGDQRLTDTR 60

Query: 61  QDEAERGITIKSTGISLYYEMSDESLKSYKGERHGNEYLINLIDSPGHVDFSSEVTAALR 120
            DE ERGITIKSTGISLYY+M+DE LK + GER GN++LINLIDSPGHVDFSSEVTAALR
Sbjct: 61  ADEQERGITIKSTGISLYYQMTDEQLKGFTGERQGNDFLINLIDSPGHVDFSSEVTAALR 120

Query: 121 ITDGALVVVDCIEGVCVQTETVLRQALGERIRPVLTVNKMDRCFLELQVDGEEAYQTFQR 180
           ITDGALVVVDCIEGVCVQTETVLRQALGERIRPVLT+NK+DRCFLEL +D EEAY  ++R
Sbjct: 121 ITDGALVVVDCIEGVCVQTETVLRQALGERIRPVLTINKIDRCFLELMLDPEEAYLAYRR 180

Query: 181 VIENANVIMATYEDPLLGDVQVYPEKGTVAFSAGLHGWAFTLTNFAKMYASKFGVDEAKM 240
           VIENANVIMATY D  LGD Q +PE GTV+FSAGLHGWAFTLT FA MYA+KFG D  +M
Sbjct: 181 VIENANVIMATYADEHLGDTQTHPEAGTVSFSAGLHGWAFTLTVFANMYAAKFGTDTKRM 240

Query: 241 MERLWGENFFDPATKKWTSKNTGTATCKRGFVQFCYEPIKQIIATCMNDRKDKLWPMLQK 300
           ME+LWG+NFFD  T+KWT K+TG  TCKRGF QF YEPIK +I   MND KDKL+ +L+K
Sbjct: 241 MEKLWGDNFFDATTRKWTKKHTGADTCKRGFCQFIYEPIKTVIEAAMNDNKDKLFDLLKK 300

Query: 301 LGVY 305
           L VY
Sbjct: 301 LNVY 304

BLAST of CSPI02G20460.1 vs. Swiss-Prot
Match: EF2_YEAST (Elongation factor 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=EFT1 PE=1 SV=1)

HSP 1 Score: 402.5 bits (1033), Expect = 4.8e-111
Identity = 199/305 (65.25%), Postives = 241/305 (79.02%), Query Frame = 1

Query: 1   MVKFTAEELRRIMDYKHNIRNMSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGDVRMTDTR 60
           MV FT +++R +MD   N+RNMSVIAHVDHGKSTLTDSLV  AGII+   AG+ R TDTR
Sbjct: 1   MVAFTVDQMRSLMDKVTNVRNMSVIAHVDHGKSTLTDSLVQRAGIISAAKAGEARFTDTR 60

Query: 61  QDEAERGITIKSTGISLYYEMSDESLKSYKGERHGNEYLINLIDSPGHVDFSSEVTAALR 120
           +DE ERGITIKST ISLY EMSDE +K  K +  GN +LINLIDSPGHVDFSSEVTAALR
Sbjct: 61  KDEQERGITIKSTAISLYSEMSDEDVKEIKQKTDGNSFLINLIDSPGHVDFSSEVTAALR 120

Query: 121 ITDGALVVVDCIEGVCVQTETVLRQALGERIRPVLTVNKMDRCFLELQVDGEEAYQTFQR 180
           +TDGALVVVD IEGVCVQTETVLRQALGERI+PV+ +NK+DR  LELQV  E+ YQTF R
Sbjct: 121 VTDGALVVVDTIEGVCVQTETVLRQALGERIKPVVVINKVDRALLELQVSKEDLYQTFAR 180

Query: 181 VIENANVIMATYEDPLLGDVQVYPEKGTVAFSAGLHGWAFTLTNFAKMYASKFGVDEAKM 240
            +E+ NVI++TY D +LGDVQVYP +GTVAF +GLHGWAFT+  FA  YA KFGVD+AKM
Sbjct: 181 TVESVNVIVSTYADEVLGDVQVYPARGTVAFGSGLHGWAFTIRQFATRYAKKFGVDKAKM 240

Query: 241 MERLWGENFFDPATKKWTSKNTGT--ATCKRGFVQFCYEPIKQIIATCMNDRKDKLWPML 300
           M+RLWG++FF+P TKKWT+K+T       +R F  F  +PI ++    MN +KD++  +L
Sbjct: 241 MDRLWGDSFFNPKTKKWTNKDTDAEGKPLERAFNMFILDPIFRLFTAIMNFKKDEIPVLL 300

Query: 301 QKLGV 304
           +KL +
Sbjct: 301 EKLEI 305

BLAST of CSPI02G20460.1 vs. Swiss-Prot
Match: EF2_PICPA (Elongation factor 2 OS=Komagataella pastoris GN=EFT1 PE=3 SV=1)

HSP 1 Score: 399.1 bits (1024), Expect = 5.3e-110
Identity = 195/309 (63.11%), Postives = 241/309 (77.99%), Query Frame = 1

Query: 1   MVKFTAEELRRIMDYKHNIRNMSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGDVRMTDTR 60
           MV FT +++R +MD   N+RNMSVIAHVDHGKSTLTDSLV  AGII+   AG+ R TDTR
Sbjct: 1   MVNFTVDQMRSLMDKVTNVRNMSVIAHVDHGKSTLTDSLVQRAGIISAAKAGEARFTDTR 60

Query: 61  QDEAERGITIKSTGISLYYEMSDESLKSYKGERHGNEYLINLIDSPGHVDFSSEVTAALR 120
           +DE ERGITIKST ISLY EM D+ +K  K +  GN +LINLIDSPGHVDFSSEVTAALR
Sbjct: 61  KDEQERGITIKSTAISLYSEMGDDDVKEIKQKTEGNSFLINLIDSPGHVDFSSEVTAALR 120

Query: 121 ITDGALVVVDCIEGVCVQTETVLRQALGERIRPVLTVNKMDRCFLELQVDGEEAYQTFQR 180
           +TDGALVVVDC+EGVCVQTETVLRQALGERI+PV+ +NK+DR  LELQV  E+ YQ+F R
Sbjct: 121 VTDGALVVVDCVEGVCVQTETVLRQALGERIKPVVVINKVDRALLELQVTKEDLYQSFAR 180

Query: 181 VIENANVIMATYEDPLLGDVQVYPEKGTVAFSAGLHGWAFTLTNFAKMYASKFGVDEAKM 240
            +E+ NV++ATY D  +GD QVYPE+GTVAF +GLHGWAFT+  FA  Y+ KFGVD  KM
Sbjct: 181 TVESVNVVIATYTDKTIGDNQVYPEQGTVAFGSGLHGWAFTVRQFATRYSKKFGVDRIKM 240

Query: 241 MERLWGENFFDPATKKWTSKNTGTA--TCKRGFVQFCYEPIKQIIATCMNDRKDKLWPML 300
           MERLWG+++F+P TKKWT+K+   A    +R F  F  +PI ++ A  MN +KD++  +L
Sbjct: 241 MERLWGDSYFNPKTKKWTNKDKDAAGKPLERAFNMFVLDPIFRLFAAIMNFKKDEIPVLL 300

Query: 301 QKLGVYVAR 308
           +KL + + R
Sbjct: 301 EKLEINLKR 309

BLAST of CSPI02G20460.1 vs. TrEMBL
Match: A0A0A0KH05_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G176930 PE=4 SV=1)

HSP 1 Score: 612.8 bits (1579), Expect = 2.6e-172
Identity = 303/303 (100.00%), Postives = 303/303 (100.00%), Query Frame = 1

Query: 1   MVKFTAEELRRIMDYKHNIRNMSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGDVRMTDTR 60
           MVKFTAEELRRIMDYKHNIRNMSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGDVRMTDTR
Sbjct: 1   MVKFTAEELRRIMDYKHNIRNMSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGDVRMTDTR 60

Query: 61  QDEAERGITIKSTGISLYYEMSDESLKSYKGERHGNEYLINLIDSPGHVDFSSEVTAALR 120
           QDEAERGITIKSTGISLYYEMSDESLKSYKGERHGNEYLINLIDSPGHVDFSSEVTAALR
Sbjct: 61  QDEAERGITIKSTGISLYYEMSDESLKSYKGERHGNEYLINLIDSPGHVDFSSEVTAALR 120

Query: 121 ITDGALVVVDCIEGVCVQTETVLRQALGERIRPVLTVNKMDRCFLELQVDGEEAYQTFQR 180
           ITDGALVVVDCIEGVCVQTETVLRQALGERIRPVLTVNKMDRCFLELQVDGEEAYQTFQR
Sbjct: 121 ITDGALVVVDCIEGVCVQTETVLRQALGERIRPVLTVNKMDRCFLELQVDGEEAYQTFQR 180

Query: 181 VIENANVIMATYEDPLLGDVQVYPEKGTVAFSAGLHGWAFTLTNFAKMYASKFGVDEAKM 240
           VIENANVIMATYEDPLLGDVQVYPEKGTVAFSAGLHGWAFTLTNFAKMYASKFGVDEAKM
Sbjct: 181 VIENANVIMATYEDPLLGDVQVYPEKGTVAFSAGLHGWAFTLTNFAKMYASKFGVDEAKM 240

Query: 241 MERLWGENFFDPATKKWTSKNTGTATCKRGFVQFCYEPIKQIIATCMNDRKDKLWPMLQK 300
           MERLWGENFFDPATKKWTSKNTGTATCKRGFVQFCYEPIKQIIATCMNDRKDKLWPMLQK
Sbjct: 241 MERLWGENFFDPATKKWTSKNTGTATCKRGFVQFCYEPIKQIIATCMNDRKDKLWPMLQK 300

Query: 301 LGV 304
           LGV
Sbjct: 301 LGV 303

BLAST of CSPI02G20460.1 vs. TrEMBL
Match: A0A0A0KC17_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G176410 PE=4 SV=1)

HSP 1 Score: 612.8 bits (1579), Expect = 2.6e-172
Identity = 303/303 (100.00%), Postives = 303/303 (100.00%), Query Frame = 1

Query: 1   MVKFTAEELRRIMDYKHNIRNMSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGDVRMTDTR 60
           MVKFTAEELRRIMDYKHNIRNMSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGDVRMTDTR
Sbjct: 1   MVKFTAEELRRIMDYKHNIRNMSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGDVRMTDTR 60

Query: 61  QDEAERGITIKSTGISLYYEMSDESLKSYKGERHGNEYLINLIDSPGHVDFSSEVTAALR 120
           QDEAERGITIKSTGISLYYEMSDESLKSYKGERHGNEYLINLIDSPGHVDFSSEVTAALR
Sbjct: 61  QDEAERGITIKSTGISLYYEMSDESLKSYKGERHGNEYLINLIDSPGHVDFSSEVTAALR 120

Query: 121 ITDGALVVVDCIEGVCVQTETVLRQALGERIRPVLTVNKMDRCFLELQVDGEEAYQTFQR 180
           ITDGALVVVDCIEGVCVQTETVLRQALGERIRPVLTVNKMDRCFLELQVDGEEAYQTFQR
Sbjct: 121 ITDGALVVVDCIEGVCVQTETVLRQALGERIRPVLTVNKMDRCFLELQVDGEEAYQTFQR 180

Query: 181 VIENANVIMATYEDPLLGDVQVYPEKGTVAFSAGLHGWAFTLTNFAKMYASKFGVDEAKM 240
           VIENANVIMATYEDPLLGDVQVYPEKGTVAFSAGLHGWAFTLTNFAKMYASKFGVDEAKM
Sbjct: 181 VIENANVIMATYEDPLLGDVQVYPEKGTVAFSAGLHGWAFTLTNFAKMYASKFGVDEAKM 240

Query: 241 MERLWGENFFDPATKKWTSKNTGTATCKRGFVQFCYEPIKQIIATCMNDRKDKLWPMLQK 300
           MERLWGENFFDPATKKWTSKNTGTATCKRGFVQFCYEPIKQIIATCMNDRKDKLWPMLQK
Sbjct: 241 MERLWGENFFDPATKKWTSKNTGTATCKRGFVQFCYEPIKQIIATCMNDRKDKLWPMLQK 300

Query: 301 LGV 304
           LGV
Sbjct: 301 LGV 303

BLAST of CSPI02G20460.1 vs. TrEMBL
Match: V7BTG3_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_005G058100g PE=4 SV=1)

HSP 1 Score: 598.6 bits (1542), Expect = 5.0e-168
Identity = 294/303 (97.03%), Postives = 301/303 (99.34%), Query Frame = 1

Query: 1   MVKFTAEELRRIMDYKHNIRNMSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGDVRMTDTR 60
           MVKFTAEELRRIMDYKHNIRNMSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGDVRMTDTR
Sbjct: 1   MVKFTAEELRRIMDYKHNIRNMSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGDVRMTDTR 60

Query: 61  QDEAERGITIKSTGISLYYEMSDESLKSYKGERHGNEYLINLIDSPGHVDFSSEVTAALR 120
            DEAERGITIKSTGISLYYEM+DESLK+YKGER+GNEYLINLIDSPGHVDFSSEVTAALR
Sbjct: 61  ADEAERGITIKSTGISLYYEMTDESLKNYKGERNGNEYLINLIDSPGHVDFSSEVTAALR 120

Query: 121 ITDGALVVVDCIEGVCVQTETVLRQALGERIRPVLTVNKMDRCFLELQVDGEEAYQTFQR 180
           ITDGALVVVDC+EGVCVQTETVLRQALGERIRPVLTVNKMDRCFLELQVDGEEAYQTFQR
Sbjct: 121 ITDGALVVVDCVEGVCVQTETVLRQALGERIRPVLTVNKMDRCFLELQVDGEEAYQTFQR 180

Query: 181 VIENANVIMATYEDPLLGDVQVYPEKGTVAFSAGLHGWAFTLTNFAKMYASKFGVDEAKM 240
           VIENANVIMATYEDPLLGDVQVYPEKGTVAFSAGLHGWAFTLTNFAKMYASKFGVDE+KM
Sbjct: 181 VIENANVIMATYEDPLLGDVQVYPEKGTVAFSAGLHGWAFTLTNFAKMYASKFGVDESKM 240

Query: 241 MERLWGENFFDPATKKWTSKNTGTATCKRGFVQFCYEPIKQIIATCMNDRKDKLWPMLQK 300
           MERLWGENFFDPATKKWTSKNTG+ATCKRGFVQFCYEPIKQII TCMND+KDKLWPMLQK
Sbjct: 241 MERLWGENFFDPATKKWTSKNTGSATCKRGFVQFCYEPIKQIINTCMNDQKDKLWPMLQK 300

Query: 301 LGV 304
           LGV
Sbjct: 301 LGV 303

BLAST of CSPI02G20460.1 vs. TrEMBL
Match: A0A061F8V4_THECC (Ribosomal protein S5/Elongation factor G/III/V family protein OS=Theobroma cacao GN=TCM_032400 PE=4 SV=1)

HSP 1 Score: 597.4 bits (1539), Expect = 1.1e-167
Identity = 293/303 (96.70%), Postives = 300/303 (99.01%), Query Frame = 1

Query: 1   MVKFTAEELRRIMDYKHNIRNMSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGDVRMTDTR 60
           MVKFTA+ELRRIMDYKHNIRNMSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGDVRMTDTR
Sbjct: 1   MVKFTADELRRIMDYKHNIRNMSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGDVRMTDTR 60

Query: 61  QDEAERGITIKSTGISLYYEMSDESLKSYKGERHGNEYLINLIDSPGHVDFSSEVTAALR 120
           QDEAERGITIKSTGISLYYEM+DESLK+YKGER GNEYLINLIDSPGHVDFSSEVTAALR
Sbjct: 61  QDEAERGITIKSTGISLYYEMTDESLKNYKGERQGNEYLINLIDSPGHVDFSSEVTAALR 120

Query: 121 ITDGALVVVDCIEGVCVQTETVLRQALGERIRPVLTVNKMDRCFLELQVDGEEAYQTFQR 180
           ITDGALVVVDC+EGVCVQTETVLRQALGERIRPVLTVNKMDRCFLELQVDGEEAYQTFQR
Sbjct: 121 ITDGALVVVDCVEGVCVQTETVLRQALGERIRPVLTVNKMDRCFLELQVDGEEAYQTFQR 180

Query: 181 VIENANVIMATYEDPLLGDVQVYPEKGTVAFSAGLHGWAFTLTNFAKMYASKFGVDEAKM 240
           VIENANVIMATYEDPLLGDVQVYPEKGTVAFSAGLHGWAFTLTNFAKMYASKFGVDE+KM
Sbjct: 181 VIENANVIMATYEDPLLGDVQVYPEKGTVAFSAGLHGWAFTLTNFAKMYASKFGVDESKM 240

Query: 241 MERLWGENFFDPATKKWTSKNTGTATCKRGFVQFCYEPIKQIIATCMNDRKDKLWPMLQK 300
           MERLWGENFFDPATKKWTSKNTG+ TCKRGFVQFCYEPIKQII TCMND+KDKLWPMLQK
Sbjct: 241 MERLWGENFFDPATKKWTSKNTGSPTCKRGFVQFCYEPIKQIINTCMNDQKDKLWPMLQK 300

Query: 301 LGV 304
           LGV
Sbjct: 301 LGV 303

BLAST of CSPI02G20460.1 vs. TrEMBL
Match: A0A0A7A534_HEVBR (Eukaryotic translation elongation factor OS=Hevea brasiliensis PE=2 SV=1)

HSP 1 Score: 595.5 bits (1534), Expect = 4.3e-167
Identity = 292/303 (96.37%), Postives = 300/303 (99.01%), Query Frame = 1

Query: 1   MVKFTAEELRRIMDYKHNIRNMSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGDVRMTDTR 60
           MVKFTAEELRRIMD+KHNIRNMSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGDVRMTDTR
Sbjct: 1   MVKFTAEELRRIMDFKHNIRNMSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGDVRMTDTR 60

Query: 61  QDEAERGITIKSTGISLYYEMSDESLKSYKGERHGNEYLINLIDSPGHVDFSSEVTAALR 120
           QDEAERGITIKSTGISLYYEMSD++LKSYKGER+GNEYLINLIDSPGHVDFSSEVTAALR
Sbjct: 61  QDEAERGITIKSTGISLYYEMSDDALKSYKGERNGNEYLINLIDSPGHVDFSSEVTAALR 120

Query: 121 ITDGALVVVDCIEGVCVQTETVLRQALGERIRPVLTVNKMDRCFLELQVDGEEAYQTFQR 180
           ITDGALVVVDC+EGVCVQTETVLRQALGERIRPVLTVNKMDRCFLELQVDGEEAYQTFQR
Sbjct: 121 ITDGALVVVDCVEGVCVQTETVLRQALGERIRPVLTVNKMDRCFLELQVDGEEAYQTFQR 180

Query: 181 VIENANVIMATYEDPLLGDVQVYPEKGTVAFSAGLHGWAFTLTNFAKMYASKFGVDEAKM 240
           VIENANVIMATYEDPLLGD QVYPEKGTVAFSAGLHGWAFTLTNFAKMYASKFGVDEAKM
Sbjct: 181 VIENANVIMATYEDPLLGDCQVYPEKGTVAFSAGLHGWAFTLTNFAKMYASKFGVDEAKM 240

Query: 241 MERLWGENFFDPATKKWTSKNTGTATCKRGFVQFCYEPIKQIIATCMNDRKDKLWPMLQK 300
           MERLWGENFFDPATKKWT+KNTG+ TCKRGFVQFCYEPIKQII TCMND+KDKLWPMLQK
Sbjct: 241 MERLWGENFFDPATKKWTTKNTGSPTCKRGFVQFCYEPIKQIINTCMNDQKDKLWPMLQK 300

Query: 301 LGV 304
           LGV
Sbjct: 301 LGV 303

BLAST of CSPI02G20460.1 vs. TAIR10
Match: AT1G56070.1 (AT1G56070.1 Ribosomal protein S5/Elongation factor G/III/V family protein)

HSP 1 Score: 580.5 bits (1495), Expect = 7.2e-166
Identity = 286/303 (94.39%), Postives = 294/303 (97.03%), Query Frame = 1

Query: 1   MVKFTAEELRRIMDYKHNIRNMSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGDVRMTDTR 60
           MVKFTA+ELRRIMDYKHNIRNMSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGDVRMTDTR
Sbjct: 1   MVKFTADELRRIMDYKHNIRNMSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGDVRMTDTR 60

Query: 61  QDEAERGITIKSTGISLYYEMSDESLKSYKGERHGNEYLINLIDSPGHVDFSSEVTAALR 120
            DEAERGITIKSTGISLYYEM+DESLKS+ G R GNEYLINLIDSPGHVDFSSEVTAALR
Sbjct: 61  ADEAERGITIKSTGISLYYEMTDESLKSFTGARDGNEYLINLIDSPGHVDFSSEVTAALR 120

Query: 121 ITDGALVVVDCIEGVCVQTETVLRQALGERIRPVLTVNKMDRCFLELQVDGEEAYQTFQR 180
           ITDGALVVVDCIEGVCVQTETVLRQALGERIRPVLTVNKMDRCFLELQVDGEEAYQTF R
Sbjct: 121 ITDGALVVVDCIEGVCVQTETVLRQALGERIRPVLTVNKMDRCFLELQVDGEEAYQTFSR 180

Query: 181 VIENANVIMATYEDPLLGDVQVYPEKGTVAFSAGLHGWAFTLTNFAKMYASKFGVDEAKM 240
           VIENANVIMATYEDPLLGDVQVYPEKGTVAFSAGLHGWAFTLTNFAKMYASKFGV E+KM
Sbjct: 181 VIENANVIMATYEDPLLGDVQVYPEKGTVAFSAGLHGWAFTLTNFAKMYASKFGVVESKM 240

Query: 241 MERLWGENFFDPATKKWTSKNTGTATCKRGFVQFCYEPIKQIIATCMNDRKDKLWPMLQK 300
           MERLWGENFFDPAT+KW+ KNTG+ TCKRGFVQFCYEPIKQIIATCMND+KDKLWPML K
Sbjct: 241 MERLWGENFFDPATRKWSGKNTGSPTCKRGFVQFCYEPIKQIIATCMNDQKDKLWPMLAK 300

Query: 301 LGV 304
           LGV
Sbjct: 301 LGV 303

BLAST of CSPI02G20460.1 vs. TAIR10
Match: AT3G12915.1 (AT3G12915.1 Ribosomal protein S5/Elongation factor G/III/V family protein)

HSP 1 Score: 518.8 bits (1335), Expect = 2.6e-147
Identity = 256/282 (90.78%), Postives = 269/282 (95.39%), Query Frame = 1

Query: 22  MSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGDVRMTDTRQDEAERGITIKSTGISLYYEM 81
           MSVIAHVDHGKSTLTDSLVAAAGIIAQE AGDVRMTDTR DEAERGITIKSTGISLYYEM
Sbjct: 1   MSVIAHVDHGKSTLTDSLVAAAGIIAQETAGDVRMTDTRADEAERGITIKSTGISLYYEM 60

Query: 82  SDESLKSYKGERHGNEYLINLIDSPGHVDFSSEVTAALRITDGALVVVDCIEGVCVQTET 141
           +D SLKS+ G R GNEYLINLIDSPGHVDFSSEVTAALRITDGALVVVDCIEGVCVQTET
Sbjct: 61  TDASLKSFTGARDGNEYLINLIDSPGHVDFSSEVTAALRITDGALVVVDCIEGVCVQTET 120

Query: 142 VLRQALGERIRPVLTVNKMDRCFLELQVDGEEAYQTFQRVIENANVIMATYEDPLLGDVQ 201
           VLRQ+LGERIRPVLTVNKMDRCFLEL+VDGEEAYQ FQRVIENANVIMAT+EDPLLGDVQ
Sbjct: 121 VLRQSLGERIRPVLTVNKMDRCFLELKVDGEEAYQNFQRVIENANVIMATHEDPLLGDVQ 180

Query: 202 VYPEKGTVAFSAGLHGWAFTLTNFAKMYASKFGVDEAKMMERLWGENFFDPATKKWTSKN 261
           VYPEKGTVAFSAGLHGWAFTLTNFAKMYASKFGV E+KMMERLWGENFFD AT+KWT+K 
Sbjct: 181 VYPEKGTVAFSAGLHGWAFTLTNFAKMYASKFGVSESKMMERLWGENFFDSATRKWTTK- 240

Query: 262 TGTATCKRGFVQFCYEPIKQIIATCMNDRKDKLWPMLQKLGV 304
           TG+ TCKRGFVQFCYEPIK +I TCMND+KDKLWPML+KLG+
Sbjct: 241 TGSPTCKRGFVQFCYEPIKIMINTCMNDQKDKLWPMLEKLGI 281

BLAST of CSPI02G20460.1 vs. TAIR10
Match: AT3G22980.1 (AT3G22980.1 Ribosomal protein S5/Elongation factor G/III/V family protein)

HSP 1 Score: 202.2 bits (513), Expect = 5.3e-52
Identity = 115/305 (37.70%), Postives = 164/305 (53.77%), Query Frame = 1

Query: 19  IRNMSVIAHVDHGKSTLTDSLVAAAG--IIAQEVAGDVRMTDTRQDEAERGITIKSTGIS 78
           +RN+ ++AHVDHGK+TL D L+A++G  ++   +AG +R  D   +E  R IT+KS+ IS
Sbjct: 9   VRNICILAHVDHGKTTLADHLIASSGGGVLHPRLAGKLRFMDYLDEEQRRAITMKSSSIS 68

Query: 79  LYYEMSDESLKSYKGERHGNEYLINLIDSPGHVDFSSEVTAALRITDGALVVVDCIEGVC 138
           L Y+                +Y +NLIDSPGH+DF SEV+ A R++DGALV+VD +EGV 
Sbjct: 69  LKYK----------------DYSLNLIDSPGHMDFCSEVSTAARLSDGALVLVDAVEGVH 128

Query: 139 VQTETVLRQALGERIRPVLTVNKMDRCFLELQVDGEEAYQTFQRVIENANVIMATYE--- 198
           +QT  VLRQA  E++ P L +NK+DR   EL++   EAY    R++   N I++ Y+   
Sbjct: 129 IQTHAVLRQAWIEKLTPCLVLNKIDRLIFELRLSPMEAYTRLIRIVHEVNGIVSAYKSEK 188

Query: 199 -----DPLLG------------------DVQVYPEKGTVAFSAGLHGWAFTLTNFAKMYA 258
                D +L                   +V   P+KG V F   L GW F +  FA  YA
Sbjct: 189 YLSDVDSILASPSGELSAESLELLEDDEEVTFQPQKGNVVFVCALDGWGFGIAEFANFYA 248

Query: 259 SKFGVDEAKMMERLWGENFFDPATKKWTSKNTGTA--TCKRGFVQFCYEPIKQIIATCMN 294
           SK G     + + LWG  ++ P TK    K   +A    K  FVQF  EP+ Q+    ++
Sbjct: 249 SKLGASATALQKSLWGPRYYIPKTKMIVGKKNLSAGSKAKPMFVQFVLEPLWQVYEAALD 297

BLAST of CSPI02G20460.1 vs. TAIR10
Match: AT1G06220.2 (AT1G06220.2 Ribosomal protein S5/Elongation factor G/III/V family protein)

HSP 1 Score: 177.6 bits (449), Expect = 1.4e-44
Identity = 108/306 (35.29%), Postives = 172/306 (56.21%), Query Frame = 1

Query: 19  IRNMSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGD---VRMTDTRQDEAERGITIKSTGI 78
           +RN++++ H+ HGK+   D LV     ++   A +   ++ TDTR DE ER I+IK+  +
Sbjct: 138 VRNVALVGHLQHGKTVFMDMLVEQTHHMSTFNAKNEKHMKYTDTRVDEQERNISIKAVPM 197

Query: 79  SLYYEMSDESLKSYKGERHGNEYLINLIDSPGHVDFSSEVTAALRITDGALVVVDCIEGV 138
           SL  E S               YL N++D+PGHV+FS E+TA+LR+ DGA+++VD  EGV
Sbjct: 198 SLVLEDS-----------RSKSYLCNIMDTPGHVNFSDEMTASLRLADGAVLIVDAAEGV 257

Query: 139 CVQTETVLRQALGERIRPVLTVNKMDRCFLELQVDGEEAYQTFQRVIENANVIMATYEDP 198
            V TE  +R A+ + +  V+ +NK+DR   EL++   +AY   +  IE  N  ++     
Sbjct: 258 MVNTERAIRHAIQDHLPIVVVINKVDRLITELKLPPRDAYYKLRHTIEVINNHISA-AST 317

Query: 199 LLGDVQ-VYPEKGTVAFSAGLHGWAFTLTNFAKMYASKFGV--DEAKMMERLWGENFFDP 258
             GD+  + P  G V F++G  GW+FTL +FAKMYA   GV  D  K   RLWG+ ++  
Sbjct: 318 TAGDLPLIDPAAGNVCFASGTAGWSFTLQSFAKMYAKLHGVAMDVDKFASRLWGDVYYHS 377

Query: 259 ATKKWTSKNTGTATCKRGFVQFCYEPIKQIIATCMNDRKDKLWPMLQKLGVYVARTV--V 317
            T+ +  ++      +R FVQF  EP+ +I +  + + K  +   L +LGV ++ +   +
Sbjct: 378 DTRVF-KRSPPVGGGERAFVQFILEPLYKIYSQVIGEHKKSVETTLAELGVTLSNSAYKL 430

BLAST of CSPI02G20460.1 vs. TAIR10
Match: AT5G25230.1 (AT5G25230.1 Ribosomal protein S5/Elongation factor G/III/V family protein)

HSP 1 Score: 176.0 bits (445), Expect = 4.1e-44
Identity = 108/306 (35.29%), Postives = 170/306 (55.56%), Query Frame = 1

Query: 19  IRNMSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGD---VRMTDTRQDEAERGITIKSTGI 78
           +RN++++ H+ HGK+   D LV     ++   A +   +R TDTR DE ER I+IK+  +
Sbjct: 124 VRNVALVGHLQHGKTVFMDMLVEQTHRMSTFNAENDKHMRYTDTRVDEQERNISIKAVPM 183

Query: 79  SLYYEMSDESLKSYKGERHGNEYLINLIDSPGHVDFSSEVTAALRITDGALVVVDCIEGV 138
           SL  E S               YL N++D+PG+V+FS E+TA+LR+ DGA+ +VD  +GV
Sbjct: 184 SLVLEDS-----------RSKSYLCNIMDTPGNVNFSDEMTASLRLADGAVFIVDAAQGV 243

Query: 139 CVQTETVLRQALGERIRPVLTVNKMDRCFLELQVDGEEAYQTFQRVIENANVIMATYEDP 198
            V TE  +R A+ + +  V+ +NK+DR   EL++   +AY   +  IE  N  ++     
Sbjct: 244 MVNTERAIRHAIQDHLPIVVVINKVDRLITELKLPPRDAYYKLRYTIEVINNHISAASTN 303

Query: 199 LLGDVQVYPEKGTVAFSAGLHGWAFTLTNFAKMYASKFGV--DEAKMMERLWGENFFDPA 258
                 + P  G V F++G  GW+FTL +FA+MYA   GV  D  K   RLWG+ ++ P 
Sbjct: 304 AADLPLIDPAAGNVCFASGTAGWSFTLQSFARMYAKLHGVAMDVDKFASRLWGDVYYHPD 363

Query: 259 TKKW-TSKNTGTATCKRGFVQFCYEPIKQIIATCMNDRKDKLWPMLQKLGVYVARTV--V 317
           T+ + TS   G    +R FVQF  EP+ +I +  + + K  +   L +LGV ++ +   +
Sbjct: 364 TRVFNTSPPVGGG--ERAFVQFILEPLYKIYSQVIGEHKKSVETTLAELGVTLSNSAYKL 416

BLAST of CSPI02G20460.1 vs. NCBI nr
Match: gi|778713730|ref|XP_011657107.1| (PREDICTED: elongation factor 2 [Cucumis sativus])

HSP 1 Score: 612.8 bits (1579), Expect = 3.7e-172
Identity = 303/303 (100.00%), Postives = 303/303 (100.00%), Query Frame = 1

Query: 1   MVKFTAEELRRIMDYKHNIRNMSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGDVRMTDTR 60
           MVKFTAEELRRIMDYKHNIRNMSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGDVRMTDTR
Sbjct: 1   MVKFTAEELRRIMDYKHNIRNMSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGDVRMTDTR 60

Query: 61  QDEAERGITIKSTGISLYYEMSDESLKSYKGERHGNEYLINLIDSPGHVDFSSEVTAALR 120
           QDEAERGITIKSTGISLYYEMSDESLKSYKGERHGNEYLINLIDSPGHVDFSSEVTAALR
Sbjct: 61  QDEAERGITIKSTGISLYYEMSDESLKSYKGERHGNEYLINLIDSPGHVDFSSEVTAALR 120

Query: 121 ITDGALVVVDCIEGVCVQTETVLRQALGERIRPVLTVNKMDRCFLELQVDGEEAYQTFQR 180
           ITDGALVVVDCIEGVCVQTETVLRQALGERIRPVLTVNKMDRCFLELQVDGEEAYQTFQR
Sbjct: 121 ITDGALVVVDCIEGVCVQTETVLRQALGERIRPVLTVNKMDRCFLELQVDGEEAYQTFQR 180

Query: 181 VIENANVIMATYEDPLLGDVQVYPEKGTVAFSAGLHGWAFTLTNFAKMYASKFGVDEAKM 240
           VIENANVIMATYEDPLLGDVQVYPEKGTVAFSAGLHGWAFTLTNFAKMYASKFGVDEAKM
Sbjct: 181 VIENANVIMATYEDPLLGDVQVYPEKGTVAFSAGLHGWAFTLTNFAKMYASKFGVDEAKM 240

Query: 241 MERLWGENFFDPATKKWTSKNTGTATCKRGFVQFCYEPIKQIIATCMNDRKDKLWPMLQK 300
           MERLWGENFFDPATKKWTSKNTGTATCKRGFVQFCYEPIKQIIATCMNDRKDKLWPMLQK
Sbjct: 241 MERLWGENFFDPATKKWTSKNTGTATCKRGFVQFCYEPIKQIIATCMNDRKDKLWPMLQK 300

Query: 301 LGV 304
           LGV
Sbjct: 301 LGV 303

BLAST of CSPI02G20460.1 vs. NCBI nr
Match: gi|778722163|ref|XP_011658418.1| (PREDICTED: LOW QUALITY PROTEIN: elongation factor 2 [Cucumis sativus])

HSP 1 Score: 612.8 bits (1579), Expect = 3.7e-172
Identity = 303/303 (100.00%), Postives = 303/303 (100.00%), Query Frame = 1

Query: 1   MVKFTAEELRRIMDYKHNIRNMSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGDVRMTDTR 60
           MVKFTAEELRRIMDYKHNIRNMSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGDVRMTDTR
Sbjct: 1   MVKFTAEELRRIMDYKHNIRNMSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGDVRMTDTR 60

Query: 61  QDEAERGITIKSTGISLYYEMSDESLKSYKGERHGNEYLINLIDSPGHVDFSSEVTAALR 120
           QDEAERGITIKSTGISLYYEMSDESLKSYKGERHGNEYLINLIDSPGHVDFSSEVTAALR
Sbjct: 61  QDEAERGITIKSTGISLYYEMSDESLKSYKGERHGNEYLINLIDSPGHVDFSSEVTAALR 120

Query: 121 ITDGALVVVDCIEGVCVQTETVLRQALGERIRPVLTVNKMDRCFLELQVDGEEAYQTFQR 180
           ITDGALVVVDCIEGVCVQTETVLRQALGERIRPVLTVNKMDRCFLELQVDGEEAYQTFQR
Sbjct: 121 ITDGALVVVDCIEGVCVQTETVLRQALGERIRPVLTVNKMDRCFLELQVDGEEAYQTFQR 180

Query: 181 VIENANVIMATYEDPLLGDVQVYPEKGTVAFSAGLHGWAFTLTNFAKMYASKFGVDEAKM 240
           VIENANVIMATYEDPLLGDVQVYPEKGTVAFSAGLHGWAFTLTNFAKMYASKFGVDEAKM
Sbjct: 181 VIENANVIMATYEDPLLGDVQVYPEKGTVAFSAGLHGWAFTLTNFAKMYASKFGVDEAKM 240

Query: 241 MERLWGENFFDPATKKWTSKNTGTATCKRGFVQFCYEPIKQIIATCMNDRKDKLWPMLQK 300
           MERLWGENFFDPATKKWTSKNTGTATCKRGFVQFCYEPIKQIIATCMNDRKDKLWPMLQK
Sbjct: 241 MERLWGENFFDPATKKWTSKNTGTATCKRGFVQFCYEPIKQIIATCMNDRKDKLWPMLQK 300

Query: 301 LGV 304
           LGV
Sbjct: 301 LGV 303

BLAST of CSPI02G20460.1 vs. NCBI nr
Match: gi|700191827|gb|KGN47031.1| (hypothetical protein Csa_6G176930 [Cucumis sativus])

HSP 1 Score: 612.8 bits (1579), Expect = 3.7e-172
Identity = 303/303 (100.00%), Postives = 303/303 (100.00%), Query Frame = 1

Query: 1   MVKFTAEELRRIMDYKHNIRNMSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGDVRMTDTR 60
           MVKFTAEELRRIMDYKHNIRNMSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGDVRMTDTR
Sbjct: 1   MVKFTAEELRRIMDYKHNIRNMSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGDVRMTDTR 60

Query: 61  QDEAERGITIKSTGISLYYEMSDESLKSYKGERHGNEYLINLIDSPGHVDFSSEVTAALR 120
           QDEAERGITIKSTGISLYYEMSDESLKSYKGERHGNEYLINLIDSPGHVDFSSEVTAALR
Sbjct: 61  QDEAERGITIKSTGISLYYEMSDESLKSYKGERHGNEYLINLIDSPGHVDFSSEVTAALR 120

Query: 121 ITDGALVVVDCIEGVCVQTETVLRQALGERIRPVLTVNKMDRCFLELQVDGEEAYQTFQR 180
           ITDGALVVVDCIEGVCVQTETVLRQALGERIRPVLTVNKMDRCFLELQVDGEEAYQTFQR
Sbjct: 121 ITDGALVVVDCIEGVCVQTETVLRQALGERIRPVLTVNKMDRCFLELQVDGEEAYQTFQR 180

Query: 181 VIENANVIMATYEDPLLGDVQVYPEKGTVAFSAGLHGWAFTLTNFAKMYASKFGVDEAKM 240
           VIENANVIMATYEDPLLGDVQVYPEKGTVAFSAGLHGWAFTLTNFAKMYASKFGVDEAKM
Sbjct: 181 VIENANVIMATYEDPLLGDVQVYPEKGTVAFSAGLHGWAFTLTNFAKMYASKFGVDEAKM 240

Query: 241 MERLWGENFFDPATKKWTSKNTGTATCKRGFVQFCYEPIKQIIATCMNDRKDKLWPMLQK 300
           MERLWGENFFDPATKKWTSKNTGTATCKRGFVQFCYEPIKQIIATCMNDRKDKLWPMLQK
Sbjct: 241 MERLWGENFFDPATKKWTSKNTGTATCKRGFVQFCYEPIKQIIATCMNDRKDKLWPMLQK 300

Query: 301 LGV 304
           LGV
Sbjct: 301 LGV 303

BLAST of CSPI02G20460.1 vs. NCBI nr
Match: gi|659113189|ref|XP_008456445.1| (PREDICTED: elongation factor 2 [Cucumis melo])

HSP 1 Score: 604.7 bits (1558), Expect = 1.0e-169
Identity = 299/303 (98.68%), Postives = 301/303 (99.34%), Query Frame = 1

Query: 1   MVKFTAEELRRIMDYKHNIRNMSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGDVRMTDTR 60
           MVKFTAEELRRIMDYKHNIRNMSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGDVRMTDTR
Sbjct: 1   MVKFTAEELRRIMDYKHNIRNMSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGDVRMTDTR 60

Query: 61  QDEAERGITIKSTGISLYYEMSDESLKSYKGERHGNEYLINLIDSPGHVDFSSEVTAALR 120
           QDEAERGITIKSTGISLYYEMSDESLKSYKGER GNEYLINLIDSPGHVDFSSEVTAALR
Sbjct: 61  QDEAERGITIKSTGISLYYEMSDESLKSYKGERQGNEYLINLIDSPGHVDFSSEVTAALR 120

Query: 121 ITDGALVVVDCIEGVCVQTETVLRQALGERIRPVLTVNKMDRCFLELQVDGEEAYQTFQR 180
           ITDGALVVVDCIEGVCVQTETVLRQALGERIRPVLTVNKMDRCFLELQVDGEEAYQTFQR
Sbjct: 121 ITDGALVVVDCIEGVCVQTETVLRQALGERIRPVLTVNKMDRCFLELQVDGEEAYQTFQR 180

Query: 181 VIENANVIMATYEDPLLGDVQVYPEKGTVAFSAGLHGWAFTLTNFAKMYASKFGVDEAKM 240
           VIENANVIMATYEDPLLGDVQVYPEKGTVAFSAGLHGWAFTLTNFAKMYASKFGVDEAKM
Sbjct: 181 VIENANVIMATYEDPLLGDVQVYPEKGTVAFSAGLHGWAFTLTNFAKMYASKFGVDEAKM 240

Query: 241 MERLWGENFFDPATKKWTSKNTGTATCKRGFVQFCYEPIKQIIATCMNDRKDKLWPMLQK 300
           MERLWGENFFDPATKKWTSKNTG+ TCKRGFVQFCYEPIKQIIATCMND+KDKLWPMLQK
Sbjct: 241 MERLWGENFFDPATKKWTSKNTGSPTCKRGFVQFCYEPIKQIIATCMNDQKDKLWPMLQK 300

Query: 301 LGV 304
           LGV
Sbjct: 301 LGV 303

BLAST of CSPI02G20460.1 vs. NCBI nr
Match: gi|593697640|ref|XP_007149293.1| (hypothetical protein PHAVU_005G058100g [Phaseolus vulgaris])

HSP 1 Score: 598.6 bits (1542), Expect = 7.2e-168
Identity = 294/303 (97.03%), Postives = 301/303 (99.34%), Query Frame = 1

Query: 1   MVKFTAEELRRIMDYKHNIRNMSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGDVRMTDTR 60
           MVKFTAEELRRIMDYKHNIRNMSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGDVRMTDTR
Sbjct: 1   MVKFTAEELRRIMDYKHNIRNMSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGDVRMTDTR 60

Query: 61  QDEAERGITIKSTGISLYYEMSDESLKSYKGERHGNEYLINLIDSPGHVDFSSEVTAALR 120
            DEAERGITIKSTGISLYYEM+DESLK+YKGER+GNEYLINLIDSPGHVDFSSEVTAALR
Sbjct: 61  ADEAERGITIKSTGISLYYEMTDESLKNYKGERNGNEYLINLIDSPGHVDFSSEVTAALR 120

Query: 121 ITDGALVVVDCIEGVCVQTETVLRQALGERIRPVLTVNKMDRCFLELQVDGEEAYQTFQR 180
           ITDGALVVVDC+EGVCVQTETVLRQALGERIRPVLTVNKMDRCFLELQVDGEEAYQTFQR
Sbjct: 121 ITDGALVVVDCVEGVCVQTETVLRQALGERIRPVLTVNKMDRCFLELQVDGEEAYQTFQR 180

Query: 181 VIENANVIMATYEDPLLGDVQVYPEKGTVAFSAGLHGWAFTLTNFAKMYASKFGVDEAKM 240
           VIENANVIMATYEDPLLGDVQVYPEKGTVAFSAGLHGWAFTLTNFAKMYASKFGVDE+KM
Sbjct: 181 VIENANVIMATYEDPLLGDVQVYPEKGTVAFSAGLHGWAFTLTNFAKMYASKFGVDESKM 240

Query: 241 MERLWGENFFDPATKKWTSKNTGTATCKRGFVQFCYEPIKQIIATCMNDRKDKLWPMLQK 300
           MERLWGENFFDPATKKWTSKNTG+ATCKRGFVQFCYEPIKQII TCMND+KDKLWPMLQK
Sbjct: 241 MERLWGENFFDPATKKWTSKNTGSATCKRGFVQFCYEPIKQIINTCMNDQKDKLWPMLQK 300

Query: 301 LGV 304
           LGV
Sbjct: 301 LGV 303

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
EF2_ARATH1.3e-16494.39Elongation factor 2 OS=Arabidopsis thaliana GN=LOS1 PE=1 SV=1[more]
EF2_BETVU1.6e-15991.42Elongation factor 2 OS=Beta vulgaris PE=2 SV=1[more]
EF2_PARKE4.7e-13576.97Elongation factor 2 OS=Parachlorella kessleri PE=2 SV=1[more]
EF2_YEAST4.8e-11165.25Elongation factor 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=... [more]
EF2_PICPA5.3e-11063.11Elongation factor 2 OS=Komagataella pastoris GN=EFT1 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KH05_CUCSA2.6e-172100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_6G176930 PE=4 SV=1[more]
A0A0A0KC17_CUCSA2.6e-172100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_6G176410 PE=4 SV=1[more]
V7BTG3_PHAVU5.0e-16897.03Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_005G058100g PE=4 SV=1[more]
A0A061F8V4_THECC1.1e-16796.70Ribosomal protein S5/Elongation factor G/III/V family protein OS=Theobroma cacao... [more]
A0A0A7A534_HEVBR4.3e-16796.37Eukaryotic translation elongation factor OS=Hevea brasiliensis PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT1G56070.17.2e-16694.39 Ribosomal protein S5/Elongation factor G/III/V family protein[more]
AT3G12915.12.6e-14790.78 Ribosomal protein S5/Elongation factor G/III/V family protein[more]
AT3G22980.15.3e-5237.70 Ribosomal protein S5/Elongation factor G/III/V family protein[more]
AT1G06220.21.4e-4435.29 Ribosomal protein S5/Elongation factor G/III/V family protein[more]
AT5G25230.14.1e-4435.29 Ribosomal protein S5/Elongation factor G/III/V family protein[more]
Match NameE-valueIdentityDescription
gi|778713730|ref|XP_011657107.1|3.7e-172100.00PREDICTED: elongation factor 2 [Cucumis sativus][more]
gi|778722163|ref|XP_011658418.1|3.7e-172100.00PREDICTED: LOW QUALITY PROTEIN: elongation factor 2 [Cucumis sativus][more]
gi|700191827|gb|KGN47031.1|3.7e-172100.00hypothetical protein Csa_6G176930 [Cucumis sativus][more]
gi|659113189|ref|XP_008456445.1|1.0e-16998.68PREDICTED: elongation factor 2 [Cucumis melo][more]
gi|593697640|ref|XP_007149293.1|7.2e-16897.03hypothetical protein PHAVU_005G058100g [Phaseolus vulgaris][more]
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
IPR000795TF_GTP-bd_dom
IPR005225Small_GTP-bd_dom
IPR027417P-loop_NTPase
Vocabulary: Molecular Function
TermDefinition
GO:0003924GTPase activity
GO:0005525GTP binding
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006448 regulation of translational elongation
cellular_component GO:0005840 ribosome
molecular_function GO:0003924 GTPase activity
molecular_function GO:0005525 GTP binding
molecular_function GO:0003746 translation elongation factor activity

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CSPI02G20460CSPI02G20460gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CSPI02G20460.1CSPI02G20460.1-proteinpolypeptide


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CSPI02G20460.1.cds6CSPI02G20460.1.cds6CDS
CSPI02G20460.1.cds5CSPI02G20460.1.cds5CDS
CSPI02G20460.1.cds4CSPI02G20460.1.cds4CDS
CSPI02G20460.1.cds3CSPI02G20460.1.cds3CDS
CSPI02G20460.1.cds2CSPI02G20460.1.cds2CDS


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CSPI02G20460.1.utr5p2CSPI02G20460.1.utr5p2five_prime_UTR
CSPI02G20460.1.utr5p1CSPI02G20460.1.utr5p1five_prime_UTR


Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000795Transcription factor, GTP-binding domainPRINTSPR00315ELONGATNFCTcoord: 153..162
score: 8.9E-16coord: 101..111
score: 8.9E-16coord: 65..73
score: 8.9E-16coord: 117..128
score: 8.9E-16coord: 21..34
score: 8.9
IPR000795Transcription factor, GTP-binding domainPROFILEPS51722G_TR_2coord: 17..253
score: 52
IPR005225Small GTP-binding protein domainTIGRFAMsTIGR00231TIGR00231coord: 19..176
score: 1.1
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3DG3DSA:3.40.50.300coord: 2..220
score: 1.5
IPR027417P-loop containing nucleoside triphosphate hydrolaseunknownSSF52540P-loop containing nucleoside triphosphate hydrolasescoord: 4..306
score: 1.64
NoneNo IPR availableGENE3DG3DSA:3.90.1430.10coord: 222..306
score: 7.4
NoneNo IPR availablePANTHERPTHR23115TRANSLATION FACTORcoord: 2..220
score: 8.9E-171coord: 243..249
score: 8.9E
NoneNo IPR availablePANTHERPTHR23115:SF136SUBFAMILY NOT NAMEDcoord: 2..220
score: 8.9E-171coord: 243..249
score: 8.9E
NoneNo IPR availablePFAMPF00009GTP_EFTUcoord: 17..227
score: 1.8