CSPI01G25180.1 (mRNA) Wild cucumber (PI 183967)

BLAST of CSPI01G25180.1 vs. Swiss-Prot
Match: CBDAS_CANSA (Cannabidiolic acid synthase OS=Cannabis sativa GN=CBDAS PE=1 SV=1)

HSP 1 Score: 499.6 bits (1285), Expect = 4.5e-140
Identity = 257/544 (47.24%), Postives = 358/544 (65.81%), Query Frame = 1

Query: 11  SLIAFIVICSS---FSLFNAASS--DKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSV 70
           S  +F  +C     F  FN  +S  +  E FL+C   + P++++  KL+YTQNN  Y SV
Sbjct: 4   STFSFWFVCKIIFFFFSFNIQTSIANPRENFLKCFSQYIPNNATNLKLVYTQNNPLYMSV 63

Query: 71  LNLSIRNHRFSTPNTPKPILIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVA 130
           LN +I N RF++  TPKP++I+TPS++SHIQ  ++CSK  GLQIR RSGGHD EG+SY++
Sbjct: 64  LNSTIHNLRFTSDTTPKPLVIVTPSHVSHIQGTILCSKKVGLQIRTRSGGHDSEGMSYIS 123

Query: 131 YLPFIVVDLINLRSITVDVKRRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGG 190
            +PF++VDL N+RSI +DV  +TAWV++ ATLGE+YY + EK+  L+   G CPTVC GG
Sbjct: 124 QVPFVIVDLRNMRSIKIDVHSQTAWVEAGATLGEVYYWVNEKNENLSLAAGYCPTVCAGG 183

Query: 191 YLSGGGYGLLLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVA 250
           +  GGGYG L+R YGLAADN+IDA+LV+ +G+  DR+SMGEDLFWA+RGGG  SFGI+VA
Sbjct: 184 HFGGGGYGPLMRNYGLAADNIIDAHLVNVHGKVLDRKSMGEDLFWALRGGGAESFGIIVA 243

Query: 251 WKVKLVPVPATVTFCSSSRTFEEDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVDG 310
           WK++LV VP +  F         + + L+++WQ + YK +K++      I   +T     
Sbjct: 244 WKIRLVAVPKSTMFSVKKIMEIHELVKLVNKWQNIAYKYDKDLLLMTHFITRNITDNQGK 303

Query: 311 KVKPVAIFYTS-FLGKANKAVKILKEKFPQLGLKKEECKEASWVESVVI---AANDFKVG 370
               +  +++S FLG  +  V ++ + FP+LG+KK +C++ SW+++++      N     
Sbjct: 304 NKTAIHTYFSSVFLGGVDSLVDLMNKSFPELGIKKTDCRQLSWIDTIIFYSGVVNYDTDN 363

Query: 371 EPIEALLNRSALIPPITSKKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPY 430
              E LL+RSA      +   KIK DYVK+P+P+     I  ++   +DIG       PY
Sbjct: 364 FNKEILLDRSA----GQNGAFKIKLDYVKKPIPESVFVQILEKL-YEEDIGAGMYALYPY 423

Query: 431 GGRMSEISESEISFSHRAGNLYKIAYLTGWEDPSMDVETRHLNWIREIYSYMAPFVSKSP 490
           GG M EISES I F HRAG LY++ Y+  WE    +   +HLNWIR IY++M P+VSK+P
Sbjct: 424 GGIMDEISESAIPFPHRAGILYELWYICSWE--KQEDNEKHLNWIRNIYNFMTPYVSKNP 483

Query: 491 RSAYVNYRDLDIGSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHE 546
           R AY+NYRDLDIG N  K  N   N+ QA  WG KY+G NF+RLV++KT VDP NFFR+E
Sbjct: 484 RLAYLNYRDLDIGINDPKNPN---NYTQARIWGEKYFGKNFDRLVKVKTLVDPNNFFRNE 537

BLAST of CSPI01G25180.1 vs. Swiss-Prot
Match: THCAS_CANSA (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa PE=1 SV=1)

HSP 1 Score: 497.3 bits (1279), Expect = 2.2e-139
Identity = 260/551 (47.19%), Postives = 359/551 (65.15%), Query Frame = 1

Query: 8   IHLSLIAFIVICSSFSLF-----NAASSDKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSY 67
           ++ S  +F  +C     F       + ++  E FL+C   H P++ +  KL+YTQ++  Y
Sbjct: 1   MNCSAFSFWFVCKIIFFFLSFHIQISIANPRENFLKCFSKHIPNNVANPKLVYTQHDQLY 60

Query: 68  SSVLNLSIRNHRFSTPNTPKPILIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLS 127
            S+LN +I+N RF +  TPKP++I+TPSN SHIQA ++CSK  GLQIR RSGGHD EG+S
Sbjct: 61  MSILNSTIQNLRFISDTTPKPLVIVTPSNNSHIQATILCSKKVGLQIRTRSGGHDAEGMS 120

Query: 128 YVAYLPFIVVDLINLRSITVDVKRRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVC 187
           Y++ +PF+VVDL N+ SI +DV  +TAWV++ ATLGE+YY I EK+  L+FPGG CPTV 
Sbjct: 121 YISQVPFVVVDLRNMHSIKIDVHSQTAWVEAGATLGEVYYWINEKNENLSFPGGYCPTVG 180

Query: 188 FGGYLSGGGYGLLLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGI 247
            GG+ SGGGYG L+R YGLAADN+IDA+LV+ +G+  DR+SMGEDLFWAIRGGGG +FGI
Sbjct: 181 VGGHFSGGGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGI 240

Query: 248 VVAWKVKLVPVPATVTFCSSSRTFE-EDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTS 307
           + AWK+KLV VP+  T  S  +  E    + L ++WQ + YK +K++      I   +T 
Sbjct: 241 IAAWKIKLVAVPSKSTIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLVLMTHFITKNITD 300

Query: 308 EVDGKVKPVAIFYTS--FLGKANKAVKILKEKFPQLGLKKEECKEASWVESVVI---AAN 367
              GK K     Y S  F G  +  V ++ + FP+LG+KK +CKE SW+++ +      N
Sbjct: 301 N-HGKNKTTVHGYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKEFSWIDTTIFYSGVVN 360

Query: 368 DFKVGEPIEALLNRSALIPPITSKKV--KIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGI 427
                   E LL+RSA       KK    IK DYVK+P+P+ A+  I  ++   +D+G  
Sbjct: 361 FNTANFKKEILLDRSA------GKKTAFSIKLDYVKKPIPETAMVKILEKL-YEEDVGAG 420

Query: 428 NVLFVPYGGRMSEISESEISFSHRAGNLYKIAYLTGWEDPSMDVETRHLNWIREIYSYMA 487
             +  PYGG M EISES I F HRAG +Y++ Y   WE    +   +H+NW+R +Y++  
Sbjct: 421 MYVLYPYGGIMEEISESAIPFPHRAGIMYELWYTASWE--KQEDNEKHINWVRSVYNFTT 480

Query: 488 PFVSKSPRSAYVNYRDLDIGSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDP 546
           P+VS++PR AY+NYRDLD+G  +    N   N+ QA  WG KY+G NFNRLV++KTKVDP
Sbjct: 481 PYVSQNPRLAYLNYRDLDLGKTNHASPN---NYTQARIWGEKYFGKNFNRLVKVKTKVDP 538

BLAST of CSPI01G25180.1 vs. Swiss-Prot
Match: CASL1_CANSA (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 490.7 bits (1262), Expect = 2.1e-137
Identity = 251/546 (45.97%), Postives = 356/546 (65.20%), Query Frame = 1

Query: 11  SLIAFIVICSSFSLF-----NAASSDKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSV 70
           S   F  +C     F       + ++  E FL+C   + P + + AKL+YTQ++  Y S+
Sbjct: 4   STFCFWYVCKIIFFFLSFNIQISIANPQENFLKCFSQYIPTNVTNAKLVYTQHDQFYMSI 63

Query: 71  LNLSIRNHRFSTPNTPKPILIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVA 130
           LN +I+N RF++  TPKP++IITP N+SHIQ  ++CSK  GLQIR RSGGHD EG+SY++
Sbjct: 64  LNSTIQNLRFTSDTTPKPLVIITPLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYIS 123

Query: 131 YLPFIVVDLINLRSITVDVKRRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGG 190
            +PF++VDL N+ S+ +DV  +TAWV++ ATLGE+YY I E +  L+FP G CPTV  GG
Sbjct: 124 QVPFVIVDLRNMHSVKIDVHSQTAWVEAGATLGEVYYWINENNENLSFPAGYCPTVGAGG 183

Query: 191 YLSGGGYGLLLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVA 250
           + SGGGYG L+R YGLAADN+IDA+LV+ +G+  DR+SMGEDLFWAIRGGGG +FGI+ A
Sbjct: 184 HFSGGGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAA 243

Query: 251 WKVKLVPVPATVTFCSSSRTFE-EDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVD 310
           WK++LV VP+  T  S  +  E  + + L+++WQ + Y  EK +      I   +T    
Sbjct: 244 WKIRLVAVPSMSTIFSVKKNMEIHELVKLVNKWQNIAYMYEKELLLFTHFITRNITDN-Q 303

Query: 311 GKVKPV--AIFYTSFLGKANKAVKILKEKFPQLGLKKEECKEASWVESVVIAANDFKVGE 370
           GK K    + F + F G  +  V ++ + FP+LG+KK +CK+ SW+++++  +       
Sbjct: 304 GKNKTTIHSYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKQLSWIDTIIFYSGVVNYNT 363

Query: 371 PI---EALLNRSALIPPITSKKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFV 430
                E LL+RS            IK DYVK+P+P+ A+  I  ++   +D+G    +F 
Sbjct: 364 TYFKKEILLDRSG----GRKAAFSIKLDYVKKPIPETAMVTILEKL-YEEDVGVGMFVFY 423

Query: 431 PYGGRMSEISESEISFSHRAGNLYKIAYLTGWEDPSMDVETRHLNWIREIYSYMAPFVSK 490
           PYGG M EISES I F HRAG +Y+I Y+  WE    +   +H+NWIR +Y++  P+VS+
Sbjct: 424 PYGGIMDEISESAIPFPHRAGIMYEIWYIASWE--KQEDNEKHINWIRNVYNFTTPYVSQ 483

Query: 491 SPRSAYVNYRDLDIGSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFR 546
           +PR AY+NYRDLD+G  + +  N   N+ QA  WG KY+G NFNRLV++KTKVDP NFFR
Sbjct: 484 NPRMAYLNYRDLDLGKTNFESPN---NYTQARIWGEKYFGKNFNRLVKVKTKVDPDNFFR 538

BLAST of CSPI01G25180.1 vs. Swiss-Prot
Match: CASL2_CANSA (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa GN=CBDAS3 PE=2 SV=1)

HSP 1 Score: 490.3 bits (1261), Expect = 2.7e-137
Identity = 252/546 (46.15%), Postives = 355/546 (65.02%), Query Frame = 1

Query: 11  SLIAFIVICSSFSLF-----NAASSDKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSV 70
           S   F  +C     F       + ++  E FL+CL  + P + + AKL+YTQ++  Y S+
Sbjct: 4   STFCFWYVCKIIFFFLSFNIQISIANPQENFLKCLSQYIPTNVTNAKLVYTQHDQFYMSI 63

Query: 71  LNLSIRNHRFSTPNTPKPILIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVA 130
           LN +++N RF++  TPKP++I TP N+SHIQ  ++CSK  GLQIR RSGGHD EG+SY++
Sbjct: 64  LNSTVQNLRFTSDTTPKPLVITTPLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYIS 123

Query: 131 YLPFIVVDLINLRSITVDVKRRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGG 190
            +PF++VDL N+ S+ +DV  +TAWV+S ATLGE+YY I E +  L+FP G CPTV  GG
Sbjct: 124 QVPFVIVDLRNMHSVKIDVHSQTAWVESGATLGEVYYWINENNENLSFPAGYCPTVGTGG 183

Query: 191 YLSGGGYGLLLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVA 250
           + SGGGYG L+R YGLAADN+IDA+LV+ +G+  DR+SMGEDLFWAIRGGGG +FGI+ A
Sbjct: 184 HFSGGGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAA 243

Query: 251 WKVKLVPVPATVTFCSSSRTFE-EDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVD 310
           WK++LV VP+  T  S  +  E  + + L+++WQ + Y  EK +      I   +T    
Sbjct: 244 WKIRLVAVPSMSTIFSVKKNMEIHELVKLVNKWQNIAYMYEKELLLFTHFITRNITDN-Q 303

Query: 311 GKVKPV--AIFYTSFLGKANKAVKILKEKFPQLGLKKEECKEASWVESVVI---AANDFK 370
           GK K    + F + F G  +  V ++ + FP+LG+KK +CK+ SW+++++      N   
Sbjct: 304 GKNKTTIHSYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKQLSWIDTIIFYSGVVNYNT 363

Query: 371 VGEPIEALLNRSALIPPITSKKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFV 430
                E LL+RS            IK DYVK+P+P+ A+  I  ++   +D+G    +F 
Sbjct: 364 TNFKKEILLDRSG----GRKAAFSIKLDYVKKPIPETAMVTILEKL-YEEDVGVGMFVFY 423

Query: 431 PYGGRMSEISESEISFSHRAGNLYKIAYLTGWEDPSMDVETRHLNWIREIYSYMAPFVSK 490
           PYGG M EISES I F HRAG  Y+I Y+  WE    +   +H+NWIR +Y++  P+VS+
Sbjct: 424 PYGGIMDEISESAIPFPHRAGITYEIWYIASWE--KQEDNEKHINWIRNVYNFTTPYVSQ 483

Query: 491 SPRSAYVNYRDLDIGSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFR 546
           +PR AY+NYRDLD+G  + +  N   N+ QA  WG KY+G NFNRLV++KTKVDP NFFR
Sbjct: 484 NPRMAYLNYRDLDLGKTNFESPN---NYTQARIWGEKYFGKNFNRLVKVKTKVDPDNFFR 538

BLAST of CSPI01G25180.1 vs. Swiss-Prot
Match: THCAI_CANSA (Inactive tetrahydrocannabinolic acid synthase OS=Cannabis sativa PE=3 SV=1)

HSP 1 Score: 488.4 bits (1256), Expect = 1.0e-136
Identity = 257/553 (46.47%), Postives = 362/553 (65.46%), Query Frame = 1

Query: 8   IHLSLIAFIVICSSFSLF-----NAASSDKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSY 67
           ++ S  +F  +C     F       + ++  E FL+C   + P++ +  K IYTQ++  Y
Sbjct: 1   MNCSAFSFWFVCKIIFFFLSFNIQISIANPQENFLKCFSEYIPNNPANPKFIYTQHDQLY 60

Query: 68  SSVLNLSIRNHRFSTPNTPKPILIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLS 127
            SVLN +I+N RF++  TPKP++I+TPSN+SHIQA+++CSK  GLQIR RSGGHD EGLS
Sbjct: 61  MSVLNSTIQNLRFTSDTTPKPLVIVTPSNVSHIQASILCSKKVGLQIRTRSGGHDAEGLS 120

Query: 128 YVAYLPFIVVDLINLRSITVDVKRRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVC 187
           Y++ +PF +VDL N+ ++ VD+  +TAWV++ ATLGE+YY I E +   +FPGG CPTV 
Sbjct: 121 YISQVPFAIVDLRNMHTVKVDIHSQTAWVEAGATLGEVYYWINEMNENFSFPGGYCPTVG 180

Query: 188 FGGYLSGGGYGLLLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGI 247
            GG+ SGGGYG L+R YGLAADN+IDA+LV+ +G+  DR+SMGEDLFWAIRGGGG +FGI
Sbjct: 181 VGGHFSGGGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGI 240

Query: 248 VVAWKVKLVPVPATVTFCSSSRTFE-EDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTS 307
           + AWK+KLV VP+  T  S  +  E    + L ++WQ + YK +K++   +LT   +  +
Sbjct: 241 IAAWKIKLVVVPSKATIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDL---MLTTHFRTRN 300

Query: 308 EVD--GKVKPVAIFYTS--FLGKANKAVKILKEKFPQLGLKKEECKEASWVESVVI---A 367
             D  GK K     Y S  FLG  +  V ++ + FP+LG+KK +CKE SW+++ +     
Sbjct: 301 ITDNHGKNKTTVHGYFSSIFLGGVDSLVDLMNKSFPELGIKKTDCKELSWIDTTIFYSGV 360

Query: 368 ANDFKVGEPIEALLNRSALIPPITSKKV--KIKSDYVKEPMPKVAIEGIWNRVNNSQDIG 427
            N        E LL+RSA       KK    IK DYVK+ +P+ A+  I  ++   +   
Sbjct: 361 VNYNTANFKKEILLDRSA------GKKTAFSIKLDYVKKLIPETAMVKILEKLYEEEVGV 420

Query: 428 GINVLFVPYGGRMSEISESEISFSHRAGNLYKIAYLTGWEDPSMDVETRHLNWIREIYSY 487
           G+ VL+ PYGG M EISES I F HRAG +Y++ Y   WE    +   +H+NW+R +Y++
Sbjct: 421 GMYVLY-PYGGIMDEISESAIPFPHRAGIMYELWYTATWE--KQEDNEKHINWVRSVYNF 480

Query: 488 MAPFVSKSPRSAYVNYRDLDIGSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKV 546
             P+VS++PR AY+NYRDLD+G  + +  N   N+ QA  WG KY+G NFNRLV++KTK 
Sbjct: 481 TTPYVSQNPRLAYLNYRDLDLGKTNPESPN---NYTQARIWGEKYFGKNFNRLVKVKTKA 538

BLAST of CSPI01G25180.1 vs. TrEMBL
Match: G8A1B3_MEDTR (Reticuline oxidase OS=Medicago truncatula GN=MTR_4g094488 PE=4 SV=1)

HSP 1 Score: 608.6 bits (1568), Expect = 7.6e-171
Identity = 302/540 (55.93%), Postives = 392/540 (72.59%), Query Frame = 1

Query: 13  IAFIVICSSFSLFNAASSDKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSVLNLSIRN 72
           I  I I  SF+ F   +S   + FLQCL  +S + +SI+K++YT+ NSSYSS+L  SI+N
Sbjct: 10  IVLIAIAFSFTSFAIDTSPHEDNFLQCLYSYSHNITSISKVVYTKTNSSYSSILKFSIQN 69

Query: 73  HRFSTPNTPKPILIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYLPFIVV 132
            RF+T  TPKP++IITP+ ISHIQ A+ICS+  G+QIRIRSGGHDFEGLS+V+ +PF+++
Sbjct: 70  LRFATNETPKPLVIITPTQISHIQTAIICSQHHGMQIRIRSGGHDFEGLSFVSNVPFVII 129

Query: 133 DLINLRSITVDVKRRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGGY 192
           DL N R I VDV+ RTAWVQS ATLGELYY+IA+KS TL FPGG CPTV  GG+ SGGGY
Sbjct: 130 DLTNFRGIDVDVENRTAWVQSGATLGELYYKIAQKSKTLGFPGGVCPTVGVGGHFSGGGY 189

Query: 193 GLLLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVP 252
           G LLRKYGLAADNVIDA+++D  G F DRE+MGEDLFWAIRGGGG SFG++V+WK+KLV 
Sbjct: 190 GTLLRKYGLAADNVIDAHIIDVKGRFLDREAMGEDLFWAIRGGGGASFGVIVSWKIKLVQ 249

Query: 253 VPATVTFCSSSRTFEEDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVDGKVKP--V 312
           VP+TVT  +  RT E++A  L+H+WQ+V +KLE+N+A  ++     + S   G+ K   +
Sbjct: 250 VPSTVTVFTVPRTLEQNATKLVHKWQFVAHKLEENLAINIILQRLDLNSSKQGEPKSTVL 309

Query: 313 AIFYTSFLGKANKAVKILKEKFPQLGLKKEECKEASWVESVVIAANDFKVGEPIEALLNR 372
           A+F + FLG  +  + +++EKFP+LGL +E+C E SW+ESV+     F  GEP+E LLNR
Sbjct: 310 ALFQSLFLGSVDNLLPLMEEKFPELGLVREDCVEMSWIESVLYLFR-FPEGEPLETLLNR 369

Query: 373 SALIPPITSKKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISE 432
           +           K KSD+VK P+P+  +EG+W   +       + VLF PYGG M +ISE
Sbjct: 370 TL----AAKDNSKAKSDFVKIPIPETGLEGLWPLFDEDGAEDVLMVLF-PYGGIMDKISE 429

Query: 433 SEISFSHRAGNLYKIAYLTGWEDPSMDVETRHLNWIREIYSYMAPFVSKSPRSAYVNYRD 492
           SEI F HR G LYKI Y   W     +VE  H+NWIR++YSYM PFVSKSPR+AY+NYRD
Sbjct: 430 SEIPFPHRYGTLYKIQYAVHWHQEGDEVEKLHINWIRKLYSYMEPFVSKSPRAAYINYRD 489

Query: 493 LDIGSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIPIALSQ 551
           LDIG N+    N  T++ QAS WG+KY+ NNF RL ++KTKVDP NFFR+EQSIP  +S+
Sbjct: 490 LDIGVNNI---NGYTSYKQASIWGVKYFKNNFKRLAKVKTKVDPLNFFRNEQSIPSHVSK 540

BLAST of CSPI01G25180.1 vs. TrEMBL
Match: V4UIF2_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10017782mg PE=4 SV=1)

HSP 1 Score: 608.6 bits (1568), Expect = 7.6e-171
Identity = 301/555 (54.23%), Postives = 413/555 (74.41%), Query Frame = 1

Query: 1   MKKSSPSIHLSLIAFIVICSSFSLFNAASSDKHEEFLQCLLHHSPHSSSIAKLIYTQNNS 60
           MK  S  I  S +A ++   SF L  AA++D HE FLQCL  HS +S+SI+K+IYT  NS
Sbjct: 1   MKPQSTMIVPSTLALLL---SFQLI-AAAADTHESFLQCLSLHSENSTSISKVIYTHYNS 60

Query: 61  SYSSVLNLSIRNHRFSTPNTPKPILIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEG 120
           SYSSVL L+++N RFSTP TPKP++IITP ++SHIQAA+ CS+  G+QIR+RSGGHD+EG
Sbjct: 61  SYSSVLELTLQNLRFSTPATPKPLVIITPIHVSHIQAAIKCSQKYGMQIRVRSGGHDYEG 120

Query: 121 LSY-VAY-LPFIVVDLINLRSITVDVKRRTAWVQSAATLGELYYRIAEKSPTLTFPGGAC 180
           LS+  +Y  PF+++D+INL SI++DV+ +TAWVQ  ATLG+LYYRIAEKSP L FP G+C
Sbjct: 121 LSFRTSYNAPFVIIDMINLSSISIDVENKTAWVQVGATLGQLYYRIAEKSPNLAFPAGSC 180

Query: 181 PTVCFGGYLSGGGYGLLLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGG 240
           P+V  GG++SGGG+G+LLRKYGLAADN++DA+++D  G F DR+SMGEDLFWAIRGGGG 
Sbjct: 181 PSVGTGGHISGGGWGILLRKYGLAADNIVDAHMIDVQGRFLDRKSMGEDLFWAIRGGGGA 240

Query: 241 SFGIVVAWKVKLVPVPATVTFCSSSRTFEEDAINLIHQWQYVGYKLEKNIAHGVLTIGGQ 300
           SFG++VAWK+KLV VP+TVT  +  R +++    L+H+WQ+V +KL +++   ++     
Sbjct: 241 SFGVIVAWKIKLVDVPSTVTVFTVRRPWDQIVTKLVHRWQHVAHKLPEDLF--IMVFLRS 300

Query: 301 MTSEVDGKVKPVAIFYTSFLGKANKAVKILKEKFPQLGLKKEECKEASWVESVVIAANDF 360
           ++S  +G     AIF + FLG   K + +++E+FP+LGL KE+C E SW++S++I  N F
Sbjct: 301 LSSGTEGNRTMEAIFQSLFLGGVEKLLPLMQERFPELGLVKEDCNETSWIQSIMI-FNGF 360

Query: 361 KVGEPIEALLNRSALIPPITSKKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLF 420
           + GE ++ LL R+        +  K KSDYV+EPMP +A+EGI+ R+ +  +     + F
Sbjct: 361 ESGESLDILLQRTNY---SDRRAFKAKSDYVREPMPGIALEGIYERL-SKVEAEAAQLFF 420

Query: 421 VPYGGRMSEISESEISFSHRAGNLYKIAYLTGWEDPSMDVETRHLNWIREIYSYMAPFVS 480
           VPYGG+MSEISES I F HRAGN+Y+I +L  W +  +D   RH+NWIRE+YSYM P+VS
Sbjct: 421 VPYGGKMSEISESAIPFPHRAGNIYEIGHLVLWAEDGIDASQRHINWIRELYSYMTPYVS 480

Query: 481 KSPRSAYVNYRDLDIGSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFF 540
           K+PR+AY+NYRDLDIG+NS+ Y    T++ +AS WG KY+ +NF+RLV +KT VDP NFF
Sbjct: 481 KNPRAAYINYRDLDIGTNSEGY----TSYKEASVWGKKYFNHNFDRLVHVKTSVDPDNFF 540

Query: 541 RHEQSIPIALSQEDN 554
           R+EQSIP   S + N
Sbjct: 541 RNEQSIPPLSSWKKN 540

BLAST of CSPI01G25180.1 vs. TrEMBL
Match: A0A067GQ97_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g009115mg PE=4 SV=1)

HSP 1 Score: 605.9 bits (1561), Expect = 4.9e-170
Identity = 299/547 (54.66%), Postives = 411/547 (75.14%), Query Frame = 1

Query: 1   MKKSSPSIHLSLIAFIVICSSFSLFNAASSDKHEEFLQCLLHHSPHSSSIAKLIYTQNNS 60
           MK  S  I  S +A ++   SF L  AA++D HE FLQCL  HS +S+SI+K+IYTQ NS
Sbjct: 1   MKPQSTMIVPSTLALLL---SFQLI-AAAADTHESFLQCLSLHSENSTSISKVIYTQYNS 60

Query: 61  SYSSVLNLSIRNHRFSTPNTPKPILIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEG 120
           SYSSVL L+++N RFSTP TPKP++IITP ++SHIQAA+ CS+  G+QIR+RSGGHD+EG
Sbjct: 61  SYSSVLELTLQNLRFSTPATPKPLVIITPIHVSHIQAAIKCSQKYGMQIRVRSGGHDYEG 120

Query: 121 LSY-VAY-LPFIVVDLINLRSITVDVKRRTAWVQSAATLGELYYRIAEKSPTLTFPGGAC 180
           LS+  +Y  PF+++D+INL SI++DV+ +TAWVQ  ATLG+LYYRIAEKSP L FP G+C
Sbjct: 121 LSFRTSYNSPFVIIDMINLSSISIDVENKTAWVQVGATLGQLYYRIAEKSPNLAFPAGSC 180

Query: 181 PTVCFGGYLSGGGYGLLLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGG 240
           P+V  GG++SGGG+G+LLRKYGLAADN++DA ++D  G F DR+SMGEDLFWAIRGGGG 
Sbjct: 181 PSVGTGGHISGGGWGILLRKYGLAADNIVDARMIDVQGRFLDRKSMGEDLFWAIRGGGGA 240

Query: 241 SFGIVVAWKVKLVPVPATVTFCSSSRTFEEDAINLIHQWQYVGYKLEKNIAHGVLTIGGQ 300
           SFG++VAWK+KLV VP+TVT  +  R +++ A  L+H+WQ+V +KL +++   ++     
Sbjct: 241 SFGVIVAWKIKLVDVPSTVTVFTVRRPWDQIATKLVHRWQHVAHKLPEDLF--IMVFLRS 300

Query: 301 MTSEVDGKVKPVAIFYTSFLGKANKAVKILKEKFPQLGLKKEECKEASWVESVVIAANDF 360
           ++S  +G     AIF + FLG   K + +++E+FP+LGL KE+C E SW++S++I  N F
Sbjct: 301 LSSGTEGNRTMEAIFQSLFLGGVEKLLPLMQERFPELGLVKEDCNETSWIQSIMI-FNGF 360

Query: 361 KVGEPIEALLNRSALIPPITSKKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLF 420
           + GE ++ LL R+        +  K KSDYV+EPMP +A+EGI+ R+ +  +     + F
Sbjct: 361 ESGESLDILLQRTNY---SDRRAFKAKSDYVREPMPGIALEGIYERL-SKVEAEAAQLFF 420

Query: 421 VPYGGRMSEISESEISFSHRAGNLYKIAYLTGWEDPSMDVETRHLNWIREIYSYMAPFVS 480
           VPYGG+MSEIS+S I F HRAGN+Y+I +L  W +  +D   RH+NWIRE+YSYM P+VS
Sbjct: 421 VPYGGKMSEISKSAIPFPHRAGNIYEIGHLVLWAEDGIDASQRHINWIRELYSYMTPYVS 480

Query: 481 KSPRSAYVNYRDLDIGSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFF 540
           K+PR+AY+NYRDLDIG+N++ Y    T++ +AS WG KY+ +NF+RLV +KT VDP NFF
Sbjct: 481 KNPRAAYINYRDLDIGTNNEGY----TSYKEASVWGKKYFNHNFDRLVHVKTSVDPDNFF 532

Query: 541 RHEQSIP 546
           R+EQSIP
Sbjct: 541 RNEQSIP 532

BLAST of CSPI01G25180.1 vs. TrEMBL
Match: V4W7E3_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10018353mg PE=4 SV=1)

HSP 1 Score: 603.2 bits (1554), Expect = 3.2e-169
Identity = 294/532 (55.26%), Postives = 404/532 (75.94%), Query Frame = 1

Query: 16  IVICSSFSLFNAASSDKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSVLNLSIRNHRF 75
           +V+  SF L  AA++D HE FLQCL  HS +S+SI+K+IYT  NSSYSSVL L+++N RF
Sbjct: 13  LVLLLSFQLI-AAAADTHESFLQCLSLHSENSTSISKVIYTHYNSSYSSVLELTLQNLRF 72

Query: 76  STPNTPKPILIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSY-VAY-LPFIVVD 135
           STP TPKP++IITP ++S IQAA+ CS+  G+QIR+RSGGHD+EGLS+  +Y  PF+++D
Sbjct: 73  STPATPKPLVIITPIHLSDIQAAIKCSQKYGMQIRVRSGGHDYEGLSFRTSYNSPFVIID 132

Query: 136 LINLRSITVDVKRRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGGYG 195
           +INL SI++DV+ +TAWVQ  ATLG+LYYRIAEKSP L FP G+CP+V  GG++SGGG+G
Sbjct: 133 MINLSSISIDVENKTAWVQVGATLGQLYYRIAEKSPNLAFPAGSCPSVGTGGHISGGGWG 192

Query: 196 LLLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPV 255
           +LLRKYGLAADN+IDA+++D  G F DR+SMGEDLFWAIRGGGG SFG++VAWK+KLV V
Sbjct: 193 ILLRKYGLAADNIIDAHMIDVQGRFLDRKSMGEDLFWAIRGGGGASFGVIVAWKIKLVDV 252

Query: 256 PATVTFCSSSRTFEEDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVDGKVKPVAIF 315
           P+TVT  +  R +++ A  L+H+WQ+V +KL +++   ++     ++S  +G     AIF
Sbjct: 253 PSTVTVFTVRRPWDQIATKLVHRWQHVAHKLPEDLF--IMVFLRSLSSGTEGNRTMEAIF 312

Query: 316 YTSFLGKANKAVKILKEKFPQLGLKKEECKEASWVESVVIAANDFKVGEPIEALLNRSAL 375
            + FLG   K + +++E+FP+LGL KE+C E SW++S++I  N F+ GE ++ LL R+  
Sbjct: 313 QSLFLGGVEKLLPLMQERFPELGLVKEDCNETSWIQSIMI-FNGFESGESLDILLQRTNY 372

Query: 376 IPPITSKKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISESEI 435
                 +  K KSDYV+EPMP +A+EGI+ R+ +  +     + FVPYGG+MSEISES I
Sbjct: 373 ---SDRRAFKAKSDYVREPMPGIALEGIYERL-SKVEAEAAQLFFVPYGGKMSEISESAI 432

Query: 436 SFSHRAGNLYKIAYLTGWEDPSMDVETRHLNWIREIYSYMAPFVSKSPRSAYVNYRDLDI 495
            F HRAGN+Y+I +L  W +  +D   RH+NWIRE+YSYM P+VSK+PR+AY+NYRDLDI
Sbjct: 433 PFPHRAGNIYEIGHLVLWAEDGIDASQRHINWIRELYSYMTPYVSKNPRAAYINYRDLDI 492

Query: 496 GSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIP 546
           G+N++ Y    T++ +AS WG KY+ +NF+RLV +KT VDP NFFR+EQSIP
Sbjct: 493 GTNNEGY----TSYKEASVWGKKYFNHNFDRLVHVKTSVDPDNFFRNEQSIP 532

BLAST of CSPI01G25180.1 vs. TrEMBL
Match: A0A061G1V4_THECC (FAD-binding Berberine family protein OS=Theobroma cacao GN=TCM_015135 PE=4 SV=1)

HSP 1 Score: 598.6 bits (1542), Expect = 7.9e-168
Identity = 300/548 (54.74%), Postives = 386/548 (70.44%), Query Frame = 1

Query: 3   KSSPSIHLSLIAFIVICSSFSLFNAASSDKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSY 62
           K   S H  + +F+   + FSL  A S+  HE FL CL  HS  SS I+K+IY QNNSSY
Sbjct: 125 KKMKSPHFLVFSFL-FAAFFSLSWATSAHTHESFLHCLSLHSEDSSFISKIIYAQNNSSY 184

Query: 63  SSVLNLSIRNHRFSTPNTPKPILIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLS 122
           SSVL  S+ N RFSTP TPKP +IITP   SH+QA + CSK  GLQ+R RSGGHDFEG+S
Sbjct: 185 SSVLEFSMHNLRFSTPTTPKPQVIITPFYESHLQATIYCSKKHGLQVRTRSGGHDFEGIS 244

Query: 123 YVAYLPFIVVDLINLRSITVDVKRRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVC 182
           YV+ + F+++DLIN RSI +DV+ + AWVQS A LGELYYRIA+KS TL FP G C TV 
Sbjct: 245 YVSKVSFVIIDLINFRSIDIDVENKVAWVQSGAILGELYYRIAKKSKTLAFPAGVCHTVG 304

Query: 183 FGGYLSGGGYGLLLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGI 242
            GGY SGGGYGLL RKYGLAADN+IDA  +D NG   DR++MGEDLFWAIRGGGGGSFGI
Sbjct: 305 VGGYFSGGGYGLLFRKYGLAADNIIDAQFIDVNGRILDRKAMGEDLFWAIRGGGGGSFGI 364

Query: 243 VVAWKVKLVPVPATVTFCSSSRTFEEDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSE 302
           V+AWK+KLVP+PATVT C  S+T E++AI L+H+WQY+ +KL       V     ++ S 
Sbjct: 365 VIAWKLKLVPIPATVTGCIVSKTLEQNAIKLVHRWQYIAHKLPDETYSSVAL--RRVNSS 424

Query: 303 VDGKVKPVAIFYTSFLGKANKAVKILKEKFPQLGLKKEECKEASWVESVVIAANDFKVGE 362
            DGK   +A F   FLG  ++ V +++E+F +LGL KE+C E SW+ES++         +
Sbjct: 425 QDGKKTVLASFRLFFLGGVDELVPLMQERFSELGLVKEDCTEMSWIESILYFGQ--LRNK 484

Query: 363 PIEALLNRSALIPPITSKKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYG 422
            ++ LL+R+    P+ S   K KSDYVKEP+P++A+EG+W+++   ++     VLFV YG
Sbjct: 485 SLDVLLDRT-FQSPLISPAFKAKSDYVKEPVPEIALEGLWSKL-YEEEAKSAGVLFVAYG 544

Query: 423 GRMSEISESEISFSHRAGNLYKIAYLTGWEDPSMDVETRHLNWIREIYSYMAPFVSKSPR 482
           G M EI E+   F HRAGNLYKI Y  GW+    +   R+++WIR +YSYM+ FVSKSPR
Sbjct: 545 GEMDEIPETATPFPHRAGNLYKILYAVGWQKEDNNNSQRYVSWIRRVYSYMSSFVSKSPR 604

Query: 483 SAYVNYRDLDIGSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQ 542
            AY+NYRDLDIG+N+       T++ QAS WG KY+ NNF++L+ +KT +DP NFFRHEQ
Sbjct: 605 EAYINYRDLDIGTNNKDN----TSYAQASVWGHKYFKNNFDKLISVKTMIDPENFFRHEQ 661

Query: 543 SIPIALSQ 551
           SIP  LS+
Sbjct: 665 SIPPLLSR 661

BLAST of CSPI01G25180.1 vs. TAIR10
Match: AT5G44440.1 (AT5G44440.1 FAD-binding Berberine family protein)

HSP 1 Score: 488.0 bits (1255), Expect = 7.6e-138
Identity = 248/539 (46.01%), Postives = 351/539 (65.12%), Query Frame = 1

Query: 12  LIAFIVICSSFSLFNAASSDKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSVLNLSIR 71
           L   ++   + S   +A    HE+FL+CL +    ++   K+I+T  +SS+ S+L+ SI+
Sbjct: 7   LFTILLFSLNISPLVSAHGSNHEDFLKCLSYRMNDNTVEPKVIHTSKDSSFFSILDSSIQ 66

Query: 72  NHRFSTPNTPKPILIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAY-LPFI 131
           N RFS   TPKP+ IITP   S +Q  + C++  G+ +R RS GH +EGLSY+AY  PF 
Sbjct: 67  NPRFSVSETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYNKPFA 126

Query: 132 VVDLINLRSITVDVKRRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGG 191
           V+DL NLRSI++DV  RT WVQ+ AT GELYY I + + +L FP G  PTV  GG  SGG
Sbjct: 127 VIDLRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQFSGG 186

Query: 192 GYGLLLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKL 251
           GYG LLRKYGLAADN+IDA +VDA+G   DR++MGED FWAIRGGGG SFG++++WKVKL
Sbjct: 187 GYGTLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWKVKL 246

Query: 252 VPVPATVTFCSSSRTFEEDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVDGKVKPV 311
           V VP+T+T     +T +++A+ +I +WQY   K+  ++         + T E   K    
Sbjct: 247 VDVPSTITVFKVQKTSKKEAVRIIKKWQYAADKVPDDLFI-------RTTLERSNKNAVH 306

Query: 312 AIFYTSFLGKANKAVKILKEKFPQLGLKKEECKEASWVESVVIAANDFKVGEPIEALLNR 371
           A+F   ++G  N  + +++EKFP+LGL+KE C+E SW+ESV+  A DF  GE +  L NR
Sbjct: 307 ALFTGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFA-DFPKGESLGVLTNR 366

Query: 372 SALIPPITSKKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISE 431
                  TS   K K D+V+EP+P+ AI+ IW R+   +   G  ++  P+GG+MSE++E
Sbjct: 367 ER-----TSLSFKGKDDFVQEPIPEAAIQEIWRRLEAPEARLG-KIILTPFGGKMSEMAE 426

Query: 432 SEISFSHRAGNLYKIAYLTGW---EDPSMDVETRHLNWIREIYSYMAPFVSKSPRSAYVN 491
            E  F HR GNLY+I Y+  W   ED +     ++L W+  +Y +M P+VSKSPR AYVN
Sbjct: 427 YETPFPHRGGNLYEIQYVAYWREEEDKNKTETDKYLKWVDSVYEFMTPYVSKSPRGAYVN 486

Query: 492 YRDLDIGSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIPI 547
           ++D+D+G      G   T +++  SWG+KY+ NNF RLV++KT+VDP +FF  EQSIP+
Sbjct: 487 FKDMDLGM---YLGKKKTKYEEGKSWGVKYFKNNFERLVRVKTRVDPTDFFCDEQSIPL 528

BLAST of CSPI01G25180.1 vs. TAIR10
Match: AT5G44410.1 (AT5G44410.1 FAD-binding Berberine family protein)

HSP 1 Score: 486.9 bits (1252), Expect = 1.7e-137
Identity = 252/540 (46.67%), Postives = 355/540 (65.74%), Query Frame = 1

Query: 13  IAFIVICSSFSLFNAASSDK--HEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSVLNLSI 72
           I F+++  S S F + S+ +  HE FL+CL H      S  ++I+T  + SY S+LN SI
Sbjct: 12  IYFLLLNLSLSHFPSISAQRTNHENFLKCLSHRINEDDS--RIIHTSKDPSYFSILNSSI 71

Query: 73  RNHRFSTPNTPKPILIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYL-PF 132
           +N RF    TPKP+ IITP   + +Q+ + C++  G+ IR RSGGHD+EGLSY+A   PF
Sbjct: 72  QNPRFFVLETPKPVSIITPVQATDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMAKSRPF 131

Query: 133 IVVDLINLRSITVDVKRRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSG 192
           +V+DL NLRSIT+DV  RT WVQS AT+GELYY I + S +L FP G  PTV  GG   G
Sbjct: 132 VVIDLRNLRSITLDVDNRTGWVQSGATIGELYYEIGKLSKSLAFPAGLYPTVGIGGQFGG 191

Query: 193 GGYGLLLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVK 252
           GGYG L+RKYGL+ADNVIDA++VDANG F DR+ MGED FWAIRGGGG SF +V++WK++
Sbjct: 192 GGYGTLMRKYGLSADNVIDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVLSWKIR 251

Query: 253 LVPVPATVTFCSSSRTFEEDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVDGKVKP 312
           L+ VP+ VT     +T E++A+++I++WQY+  K+  +     L I   +  E +     
Sbjct: 252 LLDVPSVVTVFKVVKTSEKEAVSIINKWQYIADKVPND-----LFIRAMLQKETE----V 311

Query: 313 VAIFYTSFLGKANKAVKILKEKFPQLGLKKEECKEASWVESVVIAANDFKVGEPIEALLN 372
            A F   +LG  +  + ++K+KFP+LGL+   C+E SW+ESV+     F  GE +E L  
Sbjct: 312 YASFPGLYLGPVSDLLALMKDKFPELGLEIGNCREMSWIESVLW----FIKGESMEILAK 371

Query: 373 RSALIPPITSKKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEIS 432
           R       TS+  K K D+++EP+PK AI+ +W R   + +     ++  P+GG+MSEI+
Sbjct: 372 RKR-----TSRSFKGKDDFIEEPIPKTAIQYLWRRF-EAPEARLAKIILTPFGGKMSEIA 431

Query: 433 ESEISFSHRAGNLYKIAYLTGW---EDPSMDVETRHLNWIREIYSYMAPFVSKSPRSAYV 492
           ++EI F HR GNLY+I YL  W   ED +     ++L W+  +Y +M P+VSKSPR AYV
Sbjct: 432 DNEIPFPHREGNLYEIQYLAYWSEEEDKNKTNTEKYLRWVESVYEFMTPYVSKSPRRAYV 491

Query: 493 NYRDLDIGSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIPI 547
           N+RD+D+G       N+ T +++A  WG+KY+ NNF+RLV++KT VDP +FF  EQSIPI
Sbjct: 492 NFRDIDLGMYLGL--NMKTKYEEAKVWGVKYFKNNFDRLVRVKTNVDPMDFFCDEQSIPI 528

BLAST of CSPI01G25180.1 vs. TAIR10
Match: AT4G20820.1 (AT4G20820.1 FAD-binding Berberine family protein)

HSP 1 Score: 484.6 bits (1246), Expect = 8.4e-137
Identity = 247/520 (47.50%), Postives = 347/520 (66.73%), Query Frame = 1

Query: 27  AASSDKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSVLNLSIRNHRFSTPNTPKPILI 86
           +A+      FLQCL      S+ ++K+I+T N++S+SSVL  SI+N RFS P+ PKP+LI
Sbjct: 28  SANRSNQSSFLQCLSLQLNDSNIVSKVIHTPNDTSFSSVLASSIQNQRFSAPDVPKPVLI 87

Query: 87  ITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYLPFIVVDLINLRSITVDVKR 146
           +TP   S +Q+AV C++  G+ IR RSGGHD+EGLSYV + PF+++DL NLRSITVDV  
Sbjct: 88  LTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVTHKPFVILDLRNLRSITVDVDN 147

Query: 147 RTAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGGYGLLLRKYGLAADNV 206
           R+ WVQ+ AT+GELYY I +K+ TL FP G CPTV  GG+ SGGGYG LLRK+GLAAD+V
Sbjct: 148 RSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGGHFSGGGYGTLLRKHGLAADHV 207

Query: 207 IDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTFCSSSRTF 266
           IDA +VDA G   +R  MGED FWAIRGGGG SF +V++WK+ L+ VP+TVT  + ++  
Sbjct: 208 IDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLSWKIGLINVPSTVTVFNVTKFS 267

Query: 267 EEDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVDGKVKPVAIFYTSFLGKANKAVK 326
           E+ A+ +IH+WQ+V  K+  ++   V      M       V+  A F   +LG     +K
Sbjct: 268 EQSALKIIHRWQFVADKVSDDLFIRV------MLQRYKNMVR--ASFPGLYLGSVKNLLK 327

Query: 327 ILKEKFPQLGLKKEECKEASWVESVVIAANDFKVG-EPIEALLNRSALIPPITSKKVKIK 386
           ++ ++FP+LGL++++C E SW+ESV+  A   ++G EPI  L  R+       S   K K
Sbjct: 328 MVNKEFPELGLEEDDCTEMSWIESVIWFA---ELGEEPINVLTKRTR-----ASLAFKAK 387

Query: 387 SDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISESEISFSHRAGNLYKI 446
           SD+V+EPMPK AI  +W R+    +     ++F P+GG+MSEI++ E  F HR GN+Y+I
Sbjct: 388 SDFVQEPMPKTAISKLWRRL-QEPEAEHAQLIFTPFGGKMSEIADYETPFPHRKGNIYEI 447

Query: 447 AYLTGWEDPSMDVETRHLNWIREIYSYMAPFVSKSPRSAYVNYRDLDIGSNSDKYGNIVT 506
            YL  W     DV+ +++ W+  +Y  M+ FV+KSPR AY+N RDLD+G      G   +
Sbjct: 448 QYLNYWRG---DVKEKYMRWVERVYDDMSEFVAKSPRGAYINLRDLDLGM---YVGVKRS 507

Query: 507 NHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIP 546
            +++  SWG+KY+ NNF RLV++KT VDP +FF  EQSIP
Sbjct: 508 KYEEGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIP 524

BLAST of CSPI01G25180.1 vs. TAIR10
Match: AT2G34790.1 (AT2G34790.1 FAD-binding Berberine family protein)

HSP 1 Score: 474.6 bits (1220), Expect = 8.7e-134
Identity = 239/522 (45.79%), Postives = 341/522 (65.33%), Query Frame = 1

Query: 28  ASSDKHEEFLQCLLHHSPHSSSIAKLIYT--QNNSSYSSVLNLSIRNHRFSTPNTPKPIL 87
           +SS   ++F++CL+ +S  S  I    ++  QN + +   L  + +N R+ TP+ PKP+ 
Sbjct: 25  SSSTLQQDFVKCLVDNSDVSFPITASFFSPDQNATLFKEELESTAQNLRYLTPSNPKPVF 84

Query: 88  IITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAY--LPFIVVDLINLRSITVD 147
           I  P   +H+QAAV+C+K   L +R+RSGGHD+EGLS+VA    PF++VDL  LR + VD
Sbjct: 85  IFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSFVAEDETPFVIVDLSKLRQVDVD 144

Query: 148 VKRRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGGYGLLLRKYGLAA 207
           +   +AW  + AT+GE+YYRI EKS T  FP G C ++  GG+L GG YG ++RK+GL A
Sbjct: 145 LDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLGIGGHLVGGAYGSMMRKFGLGA 204

Query: 208 DNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTFCSSS 267
           DNV+DA +VDANG+  DR +MGED+FWAIRGGGGGSFG+++AWK+KLVPVPATVT  + +
Sbjct: 205 DNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILAWKIKLVPVPATVTVFTVT 264

Query: 268 RTFEEDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVDGKVKPVAIFYTSFLGKANK 327
           +T E+D   ++++W+ +  KL+ ++   V+      T++   +   ++ +   FLG +N+
Sbjct: 265 KTLEQDGTKVLYKWEQIADKLDDDLFIRVIISPASKTTKPGNRTISMS-YQAQFLGDSNR 324

Query: 328 AVKILKEKFPQLGLKKEECKEASWVESVVIAANDFKVGEPIEALLNRSALIPPITSKKVK 387
            ++++++ FP+LGL K++C E SW++SV+  A       P EALL   +L         K
Sbjct: 325 LLQVMQKSFPELGLTKKDCTEMSWIKSVMYIAGFPNSAAP-EALLAGKSLF----KNHFK 384

Query: 388 IKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISESEISFSHRAGNLY 447
            KSD+VKEP+P   +EG+W R            ++ PYGG MS ISESEI F HR G L+
Sbjct: 385 AKSDFVKEPIPVEGLEGLWERFLEEDSP---LTIWNPYGGMMSRISESEIPFPHRNGTLF 444

Query: 448 KIAYLTGWEDPSMDVETRHLNWIREIYSYMAPFVSKSPRSAYVNYRDLDIGSNSDKYGNI 507
           KI +L+ W+D  +  E RH+ WIRE+YSYM  +VSK+PR AYVNYRDLD+G+N       
Sbjct: 445 KIQWLSTWQDGKVS-EERHMKWIREMYSYMEQYVSKNPRQAYVNYRDLDLGTNE------ 504

Query: 508 VTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIP 546
                 A  WG KYY  NF RLV+IK + DP NFFRHEQS+P
Sbjct: 505 --GETDAREWGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVP 528

BLAST of CSPI01G25180.1 vs. TAIR10
Match: AT5G44380.1 (AT5G44380.1 FAD-binding Berberine family protein)

HSP 1 Score: 474.6 bits (1220), Expect = 8.7e-134
Identity = 260/554 (46.93%), Postives = 349/554 (63.00%), Query Frame = 1

Query: 1   MKKSSPSIHLSLIAFIVICSSFSLFNAASSDK--HEEFLQCLLHHSPHSSSIAKLIYT-- 60
           M  S P   +S I   V+  SF      SS     ++F+ C+  ++  S  + K ++T  
Sbjct: 1   MGNSKPLPTISCIIVSVLYFSFYCITPTSSSASIQDQFINCVKRNTHVSFPLEKTLFTPA 60

Query: 61  QNNSSYSSVLNLSIRNHRFSTPNTPKPILIITPSNISHIQAAVICSKSQGLQIRIRSGGH 120
           +N S ++ VL  + +N +F   + PKP  I  P + S +QA++ICSK  G+  R+RSGGH
Sbjct: 61  KNVSLFNQVLESTAQNLQFLAKSMPKPGFIFRPIHQSQVQASIICSKKLGIHFRVRSGGH 120

Query: 121 DFEGLSYVAYL--PFIVVDLINLRSITVDVKRRTAWVQSAATLGELYYRIAEKSPTLTFP 180
           DFE LSYV+ +  PFI++DL  L+ I VD++  +AWVQ  ATLGELYYRIAEKS    FP
Sbjct: 121 DFEALSYVSRIEKPFILLDLSKLKQINVDIESNSAWVQPGATLGELYYRIAEKSKIHGFP 180

Query: 181 GGACPTVCFGGYLSGGGYGLLLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRG 240
            G C +V  GGY++GGGYG L+RKYGLA DNV+D  +VDANG+  DR +MGEDLFWAIRG
Sbjct: 181 AGLCTSVGIGGYMTGGGYGTLMRKYGLAGDNVLDVKMVDANGKLLDRAAMGEDLFWAIRG 240

Query: 241 GGGGSFGIVVAWKVKLVPVPATVTFCSSSRTFEEDA-INLIHQWQYVGYKLEKNI-AHGV 300
           GGG SFGIV+AWK+KLVPVP TVT  + ++T E+DA +  I +WQ +  K+ + I    V
Sbjct: 241 GGGASFGIVLAWKIKLVPVPKTVTVFTVTKTLEQDARLKTISKWQQISSKIIEEIHIRVV 300

Query: 301 LTIGGQMTSEVDGKVKPVAIFYTSFLGKANKAVKILKEKFPQLGLKKEECKEASWVESVV 360
           L   G      DG       +   FLG+    +K++++ FP+LGL +++C E SW+E+ +
Sbjct: 301 LRAAGN-----DGNKTVTMTYLGQFLGEKGTLLKVMEKAFPELGLTQKDCTEMSWIEAAL 360

Query: 361 IAANDFKVGEPIEALLNRSALIPPITSKKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIG 420
                F  G PIE LL    L  P+     K  SD+VKEP+P + ++GI+ R+      G
Sbjct: 361 FHGG-FPTGSPIEILLQ---LKSPLGKDYFKATSDFVKEPIPVIGLKGIFKRLIE----G 420

Query: 421 GINVL-FVPYGGRMSEISESEISFSHRAGNLYKIAYLTGWEDPSMDVETRHLNWIREIYS 480
               L + PYGG MS+I ES I F HR G L+KI Y   W + +    +R +NWI+EIY+
Sbjct: 421 NTTFLNWTPYGGMMSKIPESAIPFPHRNGTLFKILYYANWLE-NDKTSSRKINWIKEIYN 480

Query: 481 YMAPFVSKSPRSAYVNYRDLDIGSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTK 540
           YMAP+VS +PR AYVNYRDLD G N +   N   N  +A  WG KY+  NF+RLV+IKTK
Sbjct: 481 YMAPYVSSNPRQAYVNYRDLDFGQNKN---NAKVNFIEAKIWGPKYFKGNFDRLVKIKTK 537

Query: 541 VDPYNFFRHEQSIP 546
           VDP NFFRHEQSIP
Sbjct: 541 VDPENFFRHEQSIP 537

BLAST of CSPI01G25180.1 vs. NCBI nr
Match: gi|449435914|ref|XP_004135739.1| (PREDICTED: cannabidiolic acid synthase-like [Cucumis sativus])

HSP 1 Score: 1118.2 bits (2891), Expect = 0.0e+00
Identity = 555/558 (99.46%), Postives = 557/558 (99.82%), Query Frame = 1

Query: 1   MKKSSPSIHLSLIAFIVICSSFSLFNAASSDKHEEFLQCLLHHSPHSSSIAKLIYTQNNS 60
           MKKSSPSIHLSLIAFIVICSSFSLFNAASSDKHEEFLQCLLHHSPHSSSIAKLIYTQNNS
Sbjct: 1   MKKSSPSIHLSLIAFIVICSSFSLFNAASSDKHEEFLQCLLHHSPHSSSIAKLIYTQNNS 60

Query: 61  SYSSVLNLSIRNHRFSTPNTPKPILIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEG 120
           SYSSVLNLSIRNHRFSTPNTPKPILIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEG
Sbjct: 61  SYSSVLNLSIRNHRFSTPNTPKPILIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEG 120

Query: 121 LSYVAYLPFIVVDLINLRSITVDVKRRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPT 180
           LSYVAYLPFIVVDLINLRSITVDVKRRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPT
Sbjct: 121 LSYVAYLPFIVVDLINLRSITVDVKRRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPT 180

Query: 181 VCFGGYLSGGGYGLLLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSF 240
           VCFGGYLSGGGYGLLLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSF
Sbjct: 181 VCFGGYLSGGGYGLLLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSF 240

Query: 241 GIVVAWKVKLVPVPATVTFCSSSRTFEEDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMT 300
           GIVVAWKVKLVPVPATVTFCSSSRTFEEDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMT
Sbjct: 241 GIVVAWKVKLVPVPATVTFCSSSRTFEEDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMT 300

Query: 301 SEVDGKVKPVAIFYTSFLGKANKAVKILKEKFPQLGLKKEECKEASWVESVVIAANDFKV 360
           SEVDGKVKPVAIFYTSFLGKANKAVKILKEKFPQLGLKKEECKEASWVESVVIAANDF V
Sbjct: 301 SEVDGKVKPVAIFYTSFLGKANKAVKILKEKFPQLGLKKEECKEASWVESVVIAANDFTV 360

Query: 361 GEPIEALLNRSALIPPITSKKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVP 420
           GEP+EALLNRSALIPPITSKKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVP
Sbjct: 361 GEPVEALLNRSALIPPITSKKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVP 420

Query: 421 YGGRMSEISESEISFSHRAGNLYKIAYLTGWEDPSMDVETRHLNWIREIYSYMAPFVSKS 480
           YGGRMSEISESEISFSHRAGNL+KIAYLTGWEDPSMDVETRHLNWIREIYSYMAPFVSKS
Sbjct: 421 YGGRMSEISESEISFSHRAGNLFKIAYLTGWEDPSMDVETRHLNWIREIYSYMAPFVSKS 480

Query: 481 PRSAYVNYRDLDIGSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRH 540
           PRSAYVNYRDLDIGSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRH
Sbjct: 481 PRSAYVNYRDLDIGSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRH 540

Query: 541 EQSIPIALSQEDNLVKLV 559
           EQSIPIALSQEDNLVKLV
Sbjct: 541 EQSIPIALSQEDNLVKLV 558

BLAST of CSPI01G25180.1 vs. NCBI nr
Match: gi|778662003|ref|XP_011659204.1| (PREDICTED: cannabidiolic acid synthase-like [Cucumis sativus])

HSP 1 Score: 761.5 bits (1965), Expect = 1.0e-216
Identity = 376/541 (69.50%), Postives = 447/541 (82.62%), Query Frame = 1

Query: 13  IAFIVICSSFSLFNAASSD-KHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSVLNLSIR 72
           +AFI +CSS S++ +ASS   HEEFLQCLLHHSPHS SIAKL YT  N+SYSSVLN SIR
Sbjct: 11  LAFIAVCSSSSVWPSASSHVNHEEFLQCLLHHSPHSKSIAKLAYTPINTSYSSVLNFSIR 70

Query: 73  NHRFSTPNTPKPILIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYLPFIV 132
           N RFS PNTPKP+ IITP+NISH+QAAVICSKS GLQIRIRSGGHDFEGLSYVAY PFI+
Sbjct: 71  NLRFSIPNTPKPLFIITPTNISHVQAAVICSKSHGLQIRIRSGGHDFEGLSYVAYHPFII 130

Query: 133 VDLINLRSITVDVKRRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGG 192
           VDLI+L S+T++VK+ TAWVQS ATLGELYYRIAEKS TL FP G  PTV  GG+ SGGG
Sbjct: 131 VDLIDLSSVTIEVKQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNSPTVGVGGHFSGGG 190

Query: 193 YGLLLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLV 252
           +G LLRKYGLAADNVIDAYLVDA+G FHDR+SMGEDLFWAIRGGGGGSFGIVVAWKVKLV
Sbjct: 191 FGTLLRKYGLAADNVIDAYLVDAHGVFHDRKSMGEDLFWAIRGGGGGSFGIVVAWKVKLV 250

Query: 253 PVPATVTFCSSSRTFEEDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVDGKVKPVA 312
           PVPATVT C+ SRT EE+AI L+ QWQYV  KL++++  G+  +GG+++++ D K+ P+A
Sbjct: 251 PVPATVTICTISRTLEEEAIKLVDQWQYVANKLDEDLFLGINLLGGKISAQGD-KINPIA 310

Query: 313 IFYTSFLGKANKAVKILKEKFPQLGLKKEECKEASWVESVVIAANDFKV-GEPIEALLNR 372
           +F++ FLGKA++ + IL + FPQLGL KEECKE SW+ESVV   N  ++  +P+E LLNR
Sbjct: 311 LFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQIEDQPLEVLLNR 370

Query: 373 SALIPPITSKKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISE 432
           +    P+ +  +K+KSDYVKEP+PK  IE IW R+  SQDI G N++FVPYGGRMS+IS+
Sbjct: 371 T----PLATGNIKMKSDYVKEPIPKATIEEIWQRL-ESQDIEGANLVFVPYGGRMSQISD 430

Query: 433 SEISFSHRAGNLYKIAYLTGWEDPSMDVETRHLNWIREIYSYMAPFVSKSPRSAYVNYRD 492
           SEI FSHRAGNLYKI YLTGW +P ++ E  HLNWIR+IY YM PFVSKSPR+AYVNYRD
Sbjct: 431 SEIPFSHRAGNLYKIGYLTGWFEPGVNAEKTHLNWIRDIYGYMTPFVSKSPRAAYVNYRD 490

Query: 493 LDIGSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIPIALSQ 552
           LDIGSNS KYG   T++ +A  WGLKY+GNNFNR+V +K KVDPYNFFRHEQSIP+ L  
Sbjct: 491 LDIGSNS-KYGK--TSYKRARVWGLKYFGNNFNRMVYVKNKVDPYNFFRHEQSIPVLLKH 542

BLAST of CSPI01G25180.1 vs. NCBI nr
Match: gi|659068692|ref|XP_008445709.1| (PREDICTED: cannabidiolic acid synthase-like [Cucumis melo])

HSP 1 Score: 757.3 bits (1954), Expect = 1.9e-215
Identity = 372/540 (68.89%), Postives = 447/540 (82.78%), Query Frame = 1

Query: 13  IAFIVICSSFSLFNAASSD-KHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSVLNLSIR 72
           +AFIV+CSS S++ +ASS   HEEFL CLLHHSP S SI KL+YT  N+SYS VLN SIR
Sbjct: 11  LAFIVVCSSSSVWPSASSHVNHEEFLPCLLHHSPDSKSITKLVYTPINTSYSPVLNFSIR 70

Query: 73  NHRFSTPNTPKPILIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYLPFIV 132
           N RFSTPNTPKP+LIITPSNISH+QAAVICSKS G+QIRIRSGGHDFEGLSYVAY PF+V
Sbjct: 71  NLRFSTPNTPKPLLIITPSNISHVQAAVICSKSHGIQIRIRSGGHDFEGLSYVAYHPFVV 130

Query: 133 VDLINLRSITVDVKRRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGG 192
           VDLINL S+T++V++ TAWVQS ATLGELYYRIAEKS TL FP G CPTV  GG+ SGGG
Sbjct: 131 VDLINLSSVTIEVEQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNCPTVGVGGHFSGGG 190

Query: 193 YGLLLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLV 252
           +G LLRKYGLAADNVIDAY+VDANGEFHDR+SMGEDLFWAIRGGGGGSFGIVVAWKVKLV
Sbjct: 191 FGTLLRKYGLAADNVIDAYIVDANGEFHDRKSMGEDLFWAIRGGGGGSFGIVVAWKVKLV 250

Query: 253 PVPATVTFCSSSRTFEEDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVDGKVKPVA 312
           PVPATVT C+ SRT EE+AI LI QWQYV  KL++++  G+  +GG++ ++ D K+ P+A
Sbjct: 251 PVPATVTICTISRTLEEEAIKLIDQWQYVANKLDEDLFLGINLLGGKIVAQGD-KINPIA 310

Query: 313 IFYTSFLGKANKAVKILKEKFPQLGLKKEECKEASWVESVVIAANDFKV-GEPIEALLNR 372
           +F++ FLGKA++ + IL + FPQLGL KEECKE SW+ESVV   N  ++  +P+E LLNR
Sbjct: 311 LFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQIEDQPLEVLLNR 370

Query: 373 SALIPPITSKKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISE 432
           +    P+ +  +K+KSDYVKEP+ K  I+ +W R+  SQDI G N++F+PYGGRMS+ISE
Sbjct: 371 T----PLANGNIKMKSDYVKEPISKATIKEVWQRL-KSQDIEGANLVFIPYGGRMSQISE 430

Query: 433 SEISFSHRAGNLYKIAYLTGWEDPSMDVETRHLNWIREIYSYMAPFVSKSPRSAYVNYRD 492
           SEI FSHRAGNLYKI YLTGW +P ++ E  HLNWIR+IYSYM PFV+KSPR+AYVNYRD
Sbjct: 431 SEIPFSHRAGNLYKIGYLTGWFEPGLNAEKTHLNWIRDIYSYMTPFVTKSPRAAYVNYRD 490

Query: 493 LDIGSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIPIALSQ 551
           LDIGSNS KYG   T++ +A  WG KY+G+NF+RLV +K +VDPYNFFRHEQSIP+ L +
Sbjct: 491 LDIGSNS-KYGK--TSYKRAHVWGSKYFGSNFDRLVYVKNEVDPYNFFRHEQSIPVLLKR 541

BLAST of CSPI01G25180.1 vs. NCBI nr
Match: gi|778662006|ref|XP_011659211.1| (PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis sativus])

HSP 1 Score: 751.1 bits (1938), Expect = 1.4e-213
Identity = 376/547 (68.74%), Postives = 445/547 (81.35%), Query Frame = 1

Query: 3   KSSPSIHLSLIAFIVICSSFSLFNAASSD-KHEEFLQCLLHHSPHSSSIAKLIYTQNNSS 62
           K SP I LSL+ F+V+CSS SL+ AASS   HEEF+QCLLHHSP +  I KL++T  NSS
Sbjct: 2   KFSPLIPLSLV-FVVVCSSSSLWIAASSSINHEEFIQCLLHHSPDTYLITKLVHTPINSS 61

Query: 63  YSSVLNLSIRNHRFSTPNTPKPILIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGL 122
           +SS+LN   RN RFSTPNTPKP+LIITPSNISHIQAAV+CSKS GLQIRIRSGGHDFEGL
Sbjct: 62  FSSLLNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEGL 121

Query: 123 SYVAYLPFIVVDLINLRSITVDVKRRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPTV 182
           SYVAY  FIVVDLINL+S+TVDV++ TAWV+S ATLGELYY+I +KS TL FP G CPTV
Sbjct: 122 SYVAYHQFIVVDLINLKSVTVDVEQSTAWVESGATLGELYYKIGKKSRTLGFPAGICPTV 181

Query: 183 CFGGYLSGGGYGLLLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 242
             GG+ SGGGYG LLRKYG+AADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG
Sbjct: 182 GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 241

Query: 243 IVVAWKVKLVPVPATVTFCSSSRTFEEDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTS 302
           IVVAWKVKLVPVPATVT CS+ R+ EEDAI LI QWQYV  KLE+ +   +   GG++++
Sbjct: 242 IVVAWKVKLVPVPATVTICSAKRSLEEDAIKLIDQWQYVANKLEEELFLAIFWNGGKVSA 301

Query: 303 EVDGKVKPVAIFYTSFLGKANKAVKILKEKFPQLGLKKEECKEASWVESVVIAANDFKV- 362
           +  GK+ P+A+F+T FLG  N+ + IL + FP+LGL KEECKE SW+ES   AAN F++ 
Sbjct: 302 QEGGKINPIALFFTLFLGNVNELMAILSKTFPELGLTKEECKEKSWIESAASAANRFQIE 361

Query: 363 GEPIEALLNRSALIPPITSKKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVP 422
            +P+EALLNR+    PIT    K+KSDYVKEPM K AI+GIW R+  SQDI G+ +  +P
Sbjct: 362 DQPLEALLNRT----PITHGSFKLKSDYVKEPMTKAAIQGIWKRL-ESQDIEGVTLAVIP 421

Query: 423 YGGRMSEISESEISFSHRAGNLYKIAYLTGWEDPSMDVETRHLNWIREIYSYMAPFVSKS 482
           YGGRM++ISES+I F HRAG LY+I Y+ GWE+  ++ E RHLNWIREIYSYM PFVSKS
Sbjct: 422 YGGRMNQISESKIPFPHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMTPFVSKS 481

Query: 483 PRSAYVNYRDLDIGSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRH 542
           PR+AYVNYRDLDIGSN +KYG I  N+ QA  +G KY+GNNFNRLV++K+ VDPYNFF H
Sbjct: 482 PRAAYVNYRDLDIGSN-NKYGKI--NYKQACVFGSKYFGNNFNRLVKVKSDVDPYNFFWH 539

Query: 543 EQSIPIA 548
           EQSIP++
Sbjct: 542 EQSIPLS 539

BLAST of CSPI01G25180.1 vs. NCBI nr
Match: gi|659068694|ref|XP_008445721.1| (PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis melo])

HSP 1 Score: 748.0 bits (1930), Expect = 1.2e-212
Identity = 376/545 (68.99%), Postives = 443/545 (81.28%), Query Frame = 1

Query: 5   SPSIHLSLIAFIVICSSFSLFNAASSD-KHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYS 64
           SP + LSL+ FIV+CSS SL+ A SS  KHEEFLQCLLHHSP +SSI KL++T  NSS+S
Sbjct: 17  SPLVPLSLV-FIVVCSSSSLWAATSSSIKHEEFLQCLLHHSPDTSSINKLVHTPINSSFS 76

Query: 65  SVLNLSIRNHRFSTPNTPKPILIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSY 124
           ++ N   RN RFSTPNTPKP+LIITPSNISHIQAAVICSKS GLQIRIRSGGHDFEGLSY
Sbjct: 77  TLFNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVICSKSHGLQIRIRSGGHDFEGLSY 136

Query: 125 VAYLPFIVVDLINLRSITVDVKRRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCF 184
           VAY  FIVVDLINLRS+TVDV++ TAWVQS ATLGELYY+I +KS TL FP G CPTV  
Sbjct: 137 VAYHRFIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKSRTLGFPAGICPTVGV 196

Query: 185 GGYLSGGGYGLLLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIV 244
           GG+ SGGGYG LLRKYG+AADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG+V
Sbjct: 197 GGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGVV 256

Query: 245 VAWKVKLVPVPATVTFCSSSRTFEEDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEV 304
           VAWKVKLVPVPA+VT CS+ R+ EE+AI LI +WQYV  KLE+++   +L  GG+++++ 
Sbjct: 257 VAWKVKLVPVPASVTICSAKRSVEEEAIKLIDRWQYVANKLEEDLFLAILWNGGKVSAQG 316

Query: 305 DGKVKPVAIFYTSFLGKANKAVKILKEKFPQLGLKKEECKEASWVESVVIAANDFKV-GE 364
            GK+ P+A+F+  FLG  N+ + IL + FP+LG  KEECKE SW+ES   AAN F++  +
Sbjct: 317 GGKINPIALFFPLFLGNVNELMAILNKTFPELGFTKEECKEKSWIESAASAANRFQIEDQ 376

Query: 365 PIEALLNRSALIPPITSKKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYG 424
           P+EALLNR+   P       K+KSDYVKEPM K AIEGIW R+  SQDI G+ +  +PYG
Sbjct: 377 PLEALLNRT---PINHGSSFKLKSDYVKEPMTKTAIEGIWKRL-ESQDIEGVTLALIPYG 436

Query: 425 GRMSEISESEISFSHRAGNLYKIAYLTGWEDPSMDVETRHLNWIREIYSYMAPFVSKSPR 484
           GRMS ISES I FSHRAG LY+I Y+ GW++  ++ E RHLNWIREIYSYM PFVSKSPR
Sbjct: 437 GRMSRISESNIPFSHRAGILYQIGYILGWDEKGVEAEERHLNWIREIYSYMTPFVSKSPR 496

Query: 485 SAYVNYRDLDIGSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQ 544
           +AYVNYRDLDIGSN +KYG I  N+ QA  +G KY+GNNFNRLV++KTKVDPYNFF HEQ
Sbjct: 497 TAYVNYRDLDIGSN-NKYGKI--NYKQACVFGSKYFGNNFNRLVKVKTKVDPYNFFWHEQ 553

Query: 545 SIPIA 548
           SIP++
Sbjct: 557 SIPLS 553