Bhi09M000738 (mRNA) Wax gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Bhi09M000738
Type	mRNA
Organism	Benincasa hispida (Wax gourd)
Description	Pectin lyase-like superfamily protein, putative
Location	chr9 : 20158644 .. 20160474 (-)
Sequence length	1158

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTAAATCAGAAGCTTCTTCATCCTGCCGCCGCACCACGGTCTCTAGGTATTGGAACACTTCCGGACATCGGCGGCACAGTTAATGACATTAAGGCAAATGCTGATCTTAATGGGAATGGTGGTGCAGTTTTCGATGTTACAAAATATGGCGCTAAAGCTGATGGAAAGACTGATGATGCACAGGTGGGAATATAATATCACACAAAACATCAGTGTTTTAATTATCTTTAAACTTTTGAAGTTAATTAAATTTGAGCTGTAGTTCTTTACTACAACCTAGCTACCTTAGCTTGTTATATCTTTATCAGCAATTAGGTTTAATTACAATCGTTTTATTCCAATATTTGAGTATATCTTGAGTTGTCCACATTCTTTTTATTGTCCCCCTCCAGCTAATTACACAGTCATTTTTAGATATTAAATTCTACCCATTTTATAACTCAACCAATTTAAAATATTGTAAACATGAATGGTTTAAATATTTTAACAAATTTTCACCTTCTTTGATATATATATATATATATCCCACGTGGCAGGCATTCATGACAACCTGGATTGCAGCCTGTCGGAACACAGACGGTCCGGCGAAGTTTTTAATCCCACAAGGCACATTTCTGGTTGGTCCGGTGACTTTCGCCGGTCCGTGCAAAAGCTTCCCCATCACCCTCGAAAATCAAGGAACTGTAAAGGCCACAACTGATATCACTGAATATTCTTCTCCAGAATGGTTCTCCATTGAAGATATTACTGGTTTCATCCTCACTGGCTCCGGCGTCTTCGATGGCCAAGGCACTGCTGCTTGGCCCTACAATGACTGCAAGACAAACAACTTCTGCCAGAATCTTCCAATCGTAACTATAATAATTCTGCCTAACCCAATCACTTTTCCTTAAATTATAAACTACGAGTTCAAACACTCAAAACTACTAGAGTTGTGTCTAATATATACCTAAATTTAAAAGGAAAAAAAAAAGAACATTATAGCCCCAGGAAATAGCCCCAACACCGATGGAATGCACATTAGCACCTCAAAACTGGTCACCATTACGAATAGTGTCATTGGCACCGGTGATGACTGTGTCTCCATTGGCCACAGTACCGAGAAAATCACCGTCACCGGTGTCACTTGTGGCCCGGGACATGGTCTCAGGTTACTCTATACCTATTTTTTTAAAAAAAAAACTTATTAAGATTAAATAGACACAAAGAAAAGGATCTCAAAGCTTCTTATTCTTCTTCAGCGTTGGCAGCTTGGGCAAGTACTCGAAAGAGAAGAGTGTCTATGACGTTCTAGTAAAGAACTGCACCATTTTCAATGCCACCAATGGTGCCAGAATAAAGACATGGGCTAGCCCCGTGTCGGGGTTAGCCTCAGGAATAACCTTCGAAGACATTGTAATGTACAACGTCAAAAATCCTATAATCATCGATCAAACCTATGGCACGAAGGAGAAGAAGGTAAACAAAAAAAGCAACAATGACAAAAAGAAACTAACCATGTTAATACCGATGAGCTAGCGTAACCTTTTGAGTTCATTTGTGATTTTAATGAAGCTGCAACTAATCCCTCATGCAGGCTTCGAACTGGAAGATCAGCGACGTTCACTTCAAGAACATTCGTGGGACATCAACAACGAATGTGGCAGTGTTATTGGAGTGCAGCGAGTTGCTTCCATGCGAGGGGGTGGAGCTCAGGGACATTAACTTGTTATGGGGGCACCAAGTTGAGAAATACGACCGTTCTTTCTTCATGTTTGAATGCAAAGATTTCTACTTTTGGGGTGCAGAATCCACCACCTTGTATATGACTTTATGTGTAGTGAACTAA

mRNA sequence

ATGGTAAATCAGAAGCTTCTTCATCCTGCCGCCGCACCACGGTCTCTAGGTATTGGAACACTTCCGGACATCGGCGGCACAGTTAATGACATTAAGGCAAATGCTGATCTTAATGGGAATGGTGGTGCAGTTTTCGATGTTACAAAATATGGCGCTAAAGCTGATGGAAAGACTGATGATGCACAGGCATTCATGACAACCTGGATTGCAGCCTGTCGGAACACAGACGGTCCGGCGAAGTTTTTAATCCCACAAGGCACATTTCTGGTTGGTCCGGTGACTTTCGCCGGTCCGTGCAAAAGCTTCCCCATCACCCTCGAAAATCAAGGAACTGTAAAGGCCACAACTGATATCACTGAATATTCTTCTCCAGAATGGTTCTCCATTGAAGATATTACTGGTTTCATCCTCACTGGCTCCGGCGTCTTCGATGGCCAAGGCACTGCTGCTTGGCCCTACAATGACTGCAAGACAAACAACTTCTGCCAGAATCTTCCAATCGAAAAAAAAAAGAACATTATAGCCCCAGGAAATAGCCCCAACACCGATGGAATGCACATTAGCACCTCAAAACTGGTCACCATTACGAATAGTGTCATTGGCACCGGTGATGACTGTGTCTCCATTGGCCACAGTACCGAGAAAATCACCGTCACCGGTGTCACTTGTGGCCCGGGACATGGTCTCAGCGTTGGCAGCTTGGGCAAGTACTCGAAAGAGAAGAGTGTCTATGACGTTCTAGTAAAGAACTGCACCATTTTCAATGCCACCAATGGTGCCAGAATAAAGACATGGGCTAGCCCCGTGTCGGGGTTAGCCTCAGGAATAACCTTCGAAGACATTGTAATGTACAACGTCAAAAATCCTATAATCATCGATCAAACCTATGGCACGAAGGAGAAGAAGGCTTCGAACTGGAAGATCAGCGACGTTCACTTCAAGAACATTCGTGGGACATCAACAACGAATGTGGCAGTGTTATTGGAGTGCAGCGAGTTGCTTCCATGCGAGGGGGTGGAGCTCAGGGACATTAACTTGTTATGGGGGCACCAAGTTGAGAAATACGACCGTTCTTTCTTCATGTTTGAATGCAAAGATTTCTACTTTTGGGGTGCAGAATCCACCACCTTGTATATGACTTTATGTGTAGTGAACTAA

Coding sequence (CDS)

ATGGTAAATCAGAAGCTTCTTCATCCTGCCGCCGCACCACGGTCTCTAGGTATTGGAACACTTCCGGACATCGGCGGCACAGTTAATGACATTAAGGCAAATGCTGATCTTAATGGGAATGGTGGTGCAGTTTTCGATGTTACAAAATATGGCGCTAAAGCTGATGGAAAGACTGATGATGCACAGGCATTCATGACAACCTGGATTGCAGCCTGTCGGAACACAGACGGTCCGGCGAAGTTTTTAATCCCACAAGGCACATTTCTGGTTGGTCCGGTGACTTTCGCCGGTCCGTGCAAAAGCTTCCCCATCACCCTCGAAAATCAAGGAACTGTAAAGGCCACAACTGATATCACTGAATATTCTTCTCCAGAATGGTTCTCCATTGAAGATATTACTGGTTTCATCCTCACTGGCTCCGGCGTCTTCGATGGCCAAGGCACTGCTGCTTGGCCCTACAATGACTGCAAGACAAACAACTTCTGCCAGAATCTTCCAATCGAAAAAAAAAAGAACATTATAGCCCCAGGAAATAGCCCCAACACCGATGGAATGCACATTAGCACCTCAAAACTGGTCACCATTACGAATAGTGTCATTGGCACCGGTGATGACTGTGTCTCCATTGGCCACAGTACCGAGAAAATCACCGTCACCGGTGTCACTTGTGGCCCGGGACATGGTCTCAGCGTTGGCAGCTTGGGCAAGTACTCGAAAGAGAAGAGTGTCTATGACGTTCTAGTAAAGAACTGCACCATTTTCAATGCCACCAATGGTGCCAGAATAAAGACATGGGCTAGCCCCGTGTCGGGGTTAGCCTCAGGAATAACCTTCGAAGACATTGTAATGTACAACGTCAAAAATCCTATAATCATCGATCAAACCTATGGCACGAAGGAGAAGAAGGCTTCGAACTGGAAGATCAGCGACGTTCACTTCAAGAACATTCGTGGGACATCAACAACGAATGTGGCAGTGTTATTGGAGTGCAGCGAGTTGCTTCCATGCGAGGGGGTGGAGCTCAGGGACATTAACTTGTTATGGGGGCACCAAGTTGAGAAATACGACCGTTCTTTCTTCATGTTTGAATGCAAAGATTTCTACTTTTGGGGTGCAGAATCCACCACCTTGTATATGACTTTATGTGTAGTGAACTAA

Protein sequence

MVNQKLLHPAAAPRSLGIGTLPDIGGTVNDIKANADLNGNGGAVFDVTKYGAKADGKTDDAQAFMTTWIAACRNTDGPAKFLIPQGTFLVGPVTFAGPCKSFPITLENQGTVKATTDITEYSSPEWFSIEDITGFILTGSGVFDGQGTAAWPYNDCKTNNFCQNLPIEKKKNIIAPGNSPNTDGMHISTSKLVTITNSVIGTGDDCVSIGHSTEKITVTGVTCGPGHGLSVGSLGKYSKEKSVYDVLVKNCTIFNATNGARIKTWASPVSGLASGITFEDIVMYNVKNPIIIDQTYGTKEKKASNWKISDVHFKNIRGTSTTNVAVLLECSELLPCEGVELRDINLLWGHQVEKYDRSFFMFECKDFYFWGAESTTLYMTLCVVN

Homology

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BLAST of Bhi09M000738 vs. Swiss-Prot
Match: sp|P49062|PGLR1_ARATH (Exopolygalacturonase clone GBGE184 OS=Arabidopsis thaliana OX=3702 GN=PGA3 PE=2 SV=1)

HSP 1 Score: 283.1 bits (723), Expect = 4.6e-75
Identity = 154/344 (44.77%), Postives = 202/344 (58.72%), Query Frame = 0


Query: 38  NGNGGAVFDVTKYGAKADGKTDDAQAFMTTWIAACRNTDGPAKFLIPQGTFLVGPVTFAG 97
           N     V+D+TK+GA  DG T+  +AF+ TWI  C ++  PA  L+P+GTFL GPV FAG
Sbjct: 45  NSGNPTVYDITKFGAVGDGSTNTFKAFLNTWIQVC-DSPVPATLLVPKGTFLAGPVIFAG 104

Query: 98  PCKSFPITLENQGTVKATTDITEYSSPEWFSIEDITGFILTGSGVFDGQGTAAWPYNDCK 157
           PCKS  +T+   GT+ ATT  + Y++PEWF  E +   +LTG+G F G+G A W  + C 
Sbjct: 105 PCKS-KVTVNVIGTIIATT--SGYATPEWFLFERVDNLVLTGTGTFHGKGEAVWKADGCG 164

Query: 158 TNNFCQNLP----------------------------IEKKKNI-------IAPGNSPNT 217
               C NLP                            + K +N+        AP  SPNT
Sbjct: 165 KKVQC-NLPPTSLKFRNMKNVEINGISSVNAKAFHMFLVKTENVNIQNIKLTAPAESPNT 224

Query: 218 DGMHISTSKLVTITNSVIGTGDDCVSIGHSTEKITVTGVTCGPGHGLSVGSLGKYSKEKS 277
           DG+H+S +  V+I +S I TGDDCVS+G  +  +TV  V CGPGHGLSVGSLGKY  E+ 
Sbjct: 225 DGIHLSNADNVSILDSTIATGDDCVSVGRGSNNVTVERVICGPGHGLSVGSLGKYKNEED 284

Query: 278 VYDVLVKNCTIFNATNGARIKTWASPVSGLASGITFEDIVMYNVKNPIIIDQTYGTKEKK 337
           V  + V NCT+    NG RIKTW       A  I FE+I+M +VKNPIIIDQ YG++   
Sbjct: 285 VSGIHVNNCTMIETDNGLRIKTWGGSDPSKAVDIKFENIIMQSVKNPIIIDQNYGSRGGD 344

Query: 338 ASNWKISDVHFKNIRGTSTTNVAVLLECSELLPCEGVELRDINL 347
            S   ISD+ FKNIRGT+ T   V + CS+ +PC+GV + D+NL
Sbjct: 345 -SQVAISDILFKNIRGTTITKDVVQIMCSKSVPCQGVNVVDVNL 382

[Close]

BLAST of Bhi09M000738 vs. Swiss-Prot
Match: sp|P35339|PGLR3_MAIZE (Exopolygalacturonase OS=Zea mays OX=4577 GN=PG2C PE=2 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 3.6e-72
Identity = 155/358 (43.30%), Postives = 201/358 (56.15%), Query Frame = 0


Query: 29  NDIKANADLNGNGGAVFDVTKYGAKADGKTDDAQAFMTTWIAACRNTDGPAKFLIPQGTF 88
           ND KA    +G GG+ FD+TK GA  +GKTD  +A    W +AC  T G    LIP+G F
Sbjct: 30  NDAKA----SGPGGS-FDITKLGASGNGKTDSTKAVQEAWASACGGT-GKQTILIPKGDF 89

Query: 89  LVGPVTFAGPCKSFPITLENQGTVKATTDITEYSS-PEWFSIEDITGFILTGSGVFDGQG 148
           LVGP+ F GPCK   +T++  G + ATTD+++Y     W  I  +   ++TG G  DGQG
Sbjct: 90  LVGPLNFTGPCKG-DVTIQVNGNLLATTDLSQYKDHGNWIEILRVDNLVITGKGKLDGQG 149

Query: 149 TAAWPYNDCKTNNFCQNLP----------------------------------IEKKKNI 208
            A W  N C     C+ LP                                  + K  N+
Sbjct: 150 PAVWSKNSCVKKYDCKILPNSLVMDFVNNGEVSGITLLNSKFFHMNMYKCKDMLIKDVNV 209

Query: 209 IAPGNSPNTDGMHISTSKLVTITNSVIGTGDDCVSIGHSTEKITVTGVTCGPGHGLSVGS 268
            APG+SPNTDG+H+  S  VTITN+VIG GDDC+SIG  T K+ +TGVTCGPGHG+S+GS
Sbjct: 210 TAPGDSPNTDGIHMGDSSGVTITNTVIGVGDDCISIGPGTSKVNITGVTCGPGHGISIGS 269

Query: 269 LGKYSKEKSVYDVLVKNCTIFNATNGARIKTWASPVSGL-ASGITFEDIVMYNVKNPIII 328
           LG+Y  EK V D+ VK+CT+    NG RIK +    S L AS I +E+I M +   PIII
Sbjct: 270 LGRYKDEKDVTDINVKDCTLKKTANGVRIKAYEDAASVLTASKIHYENIKMEDSGYPIII 329

Query: 329 DQTYGTKE----KKASNWKISDVHFKNIRGTSTTNVAVLLECSELLPCEGVELRDINL 347
           D  Y   +      AS   + DV FKNI GTS+T  AV L C+  +PC GV + D+N+
Sbjct: 330 DMKYCPNKLCTANGASKVTVKDVTFKNITGTSSTPEAVNLLCTAKIPCTGVTMDDVNI 380

[Close]

BLAST of Bhi09M000738 vs. Swiss-Prot
Match: sp|Q6H9K0|PGLR2_PLAAC (Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 SV=1)

HSP 1 Score: 272.3 bits (695), Expect = 8.1e-72
Identity = 145/345 (42.03%), Postives = 201/345 (58.26%), Query Frame = 0


Query: 40  NGGAVFDVTKYGAKADGKTDDAQAFMTTWIAACRNTDGPAKFLIPQGTFLVGPVTFAGPC 99
           + G+VF+V  YGAK  G  D +QA M  W AAC  + GP+  LIP+G + +G V   GPC
Sbjct: 5   SSGSVFNVNDYGAKGAG--DISQAVMKAWKAACA-SQGPSTVLIPKGNYNMGEVAMQGPC 64

Query: 100 KSFPITLENQGTVKATTDITEYSSPEWFSIEDITGFILTGSGVFDGQGTAAWPYNDCKTN 159
           K   I  +  G VKA  D +++ S  W S   I G  ++G+G  DGQG  AW  N+C  N
Sbjct: 65  KGSKIGFQIDGVVKAPADPSKFKSDGWVSFYRIDGLTVSGTGTLDGQGQTAWAKNNCDKN 124

Query: 160 NFCQ----NLPIEKKKN------------------------------IIAPGNSPNTDGM 219
             C+    NL  +  K+                              + APG S NTDG+
Sbjct: 125 PNCKHAAMNLRFDFLKHAMVRDITSLNSKMFHINVLECEDITFQHVTVTAPGTSINTDGI 184

Query: 220 HISTSKLVTITNSVIGTGDDCVSIGHSTEKITVTGVTCGPGHGLSVGSLGKYSKEKSVYD 279
           H+  SK VTITN+ I TGDDC+SIG  ++ +T+T V CGPGHG+S+GSLG+Y+ EK V  
Sbjct: 185 HVGISKGVTITNTKIATGDDCISIGPGSQNVTITQVNCGPGHGISIGSLGRYNNEKEVRG 244

Query: 280 VLVKNCTIFNATNGARIKTWASPVSGLASGITFEDIVMYNVKNPIIIDQ---TYGTKEKK 339
           + VK CT     NG R+KTW +   G A+ +TF+D+ M NV+NP+I+DQ    YG   ++
Sbjct: 245 ITVKGCTFSGTMNGVRVKTWPNSPPGAATDLTFQDLTMNNVQNPVILDQEYCPYGQCSRQ 304

Query: 340 A-SNWKISDVHFKNIRGTSTTNVAVLLECSELLPCEGVELRDINL 347
           A S  K+S+++F NIRGTST  VAV++ CS  +PC  +++ +INL
Sbjct: 305 APSRIKLSNINFNNIRGTSTGKVAVVIACSHGMPCSNMKIGEINL 346

[Close]

BLAST of Bhi09M000738 vs. Swiss-Prot
Match: sp|Q39766|PGLR_GOSBA (Polygalacturonase OS=Gossypium barbadense OX=3634 GN=G9 PE=2 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 3.4e-70
Identity = 141/339 (41.59%), Postives = 193/339 (56.93%), Query Frame = 0


Query: 47  VTKYGAKADGKTDDAQAFMTTWIAACRNTDGPAKFLIPQGTFLVGPVTFAGPCKSFPITL 106
           V K+GAKADGKTD ++ F+  W  AC +   P+  +IP+GT+L+  V   GPCK+ PI +
Sbjct: 32  VAKFGAKADGKTDLSKPFLDAWKEACASVT-PSTVVIPKGTYLLSKVNLEGPCKA-PIEI 91

Query: 107 ENQGTVKATTDITEYSSPEWFSIEDITGFILTGSGVFDGQGTAAWPYNDCKTNNF----- 166
             QGT++A  D + +  P W     +  F + G G+FDGQG+ A+  N C+   F     
Sbjct: 92  NVQGTIQAPADPSAFKDPNWVRFYSVENFKMFGGGIFDGQGSIAYEKNTCENREFRSKLP 151

Query: 167 ------------------------------CQNLPIEKKKNIIAPGNSPNTDGMHISTSK 226
                                         C+N+ +E+ K I AP  SPNTDG+H+  S+
Sbjct: 152 VNIRFDFVTNALIQDITSKDSKLFHINVFACKNITLERLK-IEAPDESPNTDGIHMGKSE 211

Query: 227 LVTITNSVIGTGDDCVSIGHSTEKITVTGVTCGPGHGLSVGSLGKYSKEKSVYDVLVKNC 286
            V I  S I TGDDC+SIG  T+ + +  +TCGPGHG+S+GSLGK+  E+ V  + + NC
Sbjct: 212 GVNIIASDIKTGDDCISIGDGTKNMVIKEITCGPGHGISIGSLGKFQNEEPVEGIKISNC 271

Query: 287 TIFNATNGARIKTWASPVSGLASGITFEDIVMYNVKNPIIIDQTY----GTKEKKASNWK 346
           TI N +NGARIKTW     G  S I FEDI M NV +PI+IDQ Y      K+ + S  K
Sbjct: 272 TITNTSNGARIKTWPGEHGGAVSEIHFEDITMNNVSSPILIDQQYCPWNKCKKNEESKVK 331

[Close]

BLAST of Bhi09M000738 vs. Swiss-Prot
Match: sp|Q39786|PGLR_GOSHI (Polygalacturonase OS=Gossypium hirsutum OX=3635 GN=G9 PE=2 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 3.4e-70
Identity = 141/339 (41.59%), Postives = 193/339 (56.93%), Query Frame = 0


Query: 47  VTKYGAKADGKTDDAQAFMTTWIAACRNTDGPAKFLIPQGTFLVGPVTFAGPCKSFPITL 106
           V K+GAKADGKTD ++ F+  W  AC +   P+  +IP+GT+L+  V   GPCK+ PI +
Sbjct: 32  VAKFGAKADGKTDLSKPFLDAWKEACASVT-PSTVVIPKGTYLLSKVNLEGPCKA-PIEI 91

Query: 107 ENQGTVKATTDITEYSSPEWFSIEDITGFILTGSGVFDGQGTAAWPYNDCKTNNF----- 166
             QGT++A  D + +  P W     +  F + G G+FDGQG+ A+  N C+   F     
Sbjct: 92  NVQGTIQAPADPSAFKDPNWVRFYSVENFKMFGGGIFDGQGSIAYEKNTCENREFRSKLP 151

Query: 167 ------------------------------CQNLPIEKKKNIIAPGNSPNTDGMHISTSK 226
                                         C+N+ +E+ K I AP  SPNTDG+H+  S+
Sbjct: 152 VNIRFDFLTNALIQDITSKDSKLFHINVFACKNITLERLK-IEAPDESPNTDGIHMGKSE 211

Query: 227 LVTITNSVIGTGDDCVSIGHSTEKITVTGVTCGPGHGLSVGSLGKYSKEKSVYDVLVKNC 286
            V I  S I TGDDC+SIG  T+ + +  +TCGPGHG+S+GSLGK+  E+ V  + + NC
Sbjct: 212 GVNIIASDIKTGDDCISIGDGTKNMVIKEITCGPGHGISIGSLGKFQNEEPVEGIKISNC 271

Query: 287 TIFNATNGARIKTWASPVSGLASGITFEDIVMYNVKNPIIIDQTY----GTKEKKASNWK 346
           TI N +NGARIKTW     G  S I FEDI M NV +PI+IDQ Y      K+ + S  K
Sbjct: 272 TITNTSNGARIKTWPGEHGGAVSEIHFEDITMNNVSSPILIDQQYCPWNKCKKNEESKVK 331

[Close]

BLAST of Bhi09M000738 vs. TAIR10
Match: AT1G02790.1 (polygalacturonase 4)

HSP 1 Score: 283.1 bits (723), Expect = 2.6e-76
Identity = 154/344 (44.77%), Postives = 202/344 (58.72%), Query Frame = 0


Query: 38  NGNGGAVFDVTKYGAKADGKTDDAQAFMTTWIAACRNTDGPAKFLIPQGTFLVGPVTFAG 97
           N     V+D+TK+GA  DG T+  +AF+ TWI  C ++  PA  L+P+GTFL GPV FAG
Sbjct: 45  NSGNPTVYDITKFGAVGDGSTNTFKAFLNTWIQVC-DSPVPATLLVPKGTFLAGPVIFAG 104

Query: 98  PCKSFPITLENQGTVKATTDITEYSSPEWFSIEDITGFILTGSGVFDGQGTAAWPYNDCK 157
           PCKS  +T+   GT+ ATT  + Y++PEWF  E +   +LTG+G F G+G A W  + C 
Sbjct: 105 PCKS-KVTVNVIGTIIATT--SGYATPEWFLFERVDNLVLTGTGTFHGKGEAVWKADGCG 164

Query: 158 TNNFCQNLP----------------------------IEKKKNI-------IAPGNSPNT 217
               C NLP                            + K +N+        AP  SPNT
Sbjct: 165 KKVQC-NLPPTSLKFRNMKNVEINGISSVNAKAFHMFLVKTENVNIQNIKLTAPAESPNT 224

Query: 218 DGMHISTSKLVTITNSVIGTGDDCVSIGHSTEKITVTGVTCGPGHGLSVGSLGKYSKEKS 277
           DG+H+S +  V+I +S I TGDDCVS+G  +  +TV  V CGPGHGLSVGSLGKY  E+ 
Sbjct: 225 DGIHLSNADNVSILDSTIATGDDCVSVGRGSNNVTVERVICGPGHGLSVGSLGKYKNEED 284

Query: 278 VYDVLVKNCTIFNATNGARIKTWASPVSGLASGITFEDIVMYNVKNPIIIDQTYGTKEKK 337
           V  + V NCT+    NG RIKTW       A  I FE+I+M +VKNPIIIDQ YG++   
Sbjct: 285 VSGIHVNNCTMIETDNGLRIKTWGGSDPSKAVDIKFENIIMQSVKNPIIIDQNYGSRGGD 344

Query: 338 ASNWKISDVHFKNIRGTSTTNVAVLLECSELLPCEGVELRDINL 347
            S   ISD+ FKNIRGT+ T   V + CS+ +PC+GV + D+NL
Sbjct: 345 -SQVAISDILFKNIRGTTITKDVVQIMCSKSVPCQGVNVVDVNL 382

[Close]

BLAST of Bhi09M000738 vs. TAIR10
Match: AT4G18180.1 (Pectin lyase-like superfamily protein)

HSP 1 Score: 268.9 bits (686), Expect = 5.0e-72
Identity = 147/342 (42.98%), Postives = 200/342 (58.48%), Query Frame = 0


Query: 46  DVTKYGAKADGKTDDAQAFMTTWIAACRNTDGPAKFLIPQGTFLVGPVTFAGPCKSFP-I 105
           DV  +GA+A+   D  +AF+  W  AC+++      +IP+G F VG + F+GPC +   +
Sbjct: 36  DVRSFGARANDHRDHTKAFVAAWDKACKSSSSSVNLIIPRGEFSVGSLRFSGPCTNVSNL 95

Query: 106 TLENQGTVKATTDITEY-SSPEWFSIEDITGFILTGSGVFDGQGTAAWPYNDCKTNNFCQ 165
           T+     VKA+TD+++Y S   W     I G  LTG G FDGQG  AWP+N+C +++ C+
Sbjct: 96  TVR----VKASTDLSKYRSGGGWIQFGWINGLTLTGGGTFDGQGALAWPFNNCTSDSNCK 155

Query: 166 NLPIEKK----------------------------------KNIIAPGNSPNTDGMHIST 225
            LP   K                                   NI AP +SPNTDG+HI  
Sbjct: 156 LLPTSLKFVGMNRTVVRRISSVNSKFFHIALVECRDFKGTRLNITAPSDSPNTDGIHIER 215

Query: 226 SKLVTITNSVIGTGDDCVSIGHSTEKITVTGVTCGPGHGLSVGSLGKYSKEKSVYDVLVK 285
           S  V  + S I TGDDCVSIG    +IT+T + CGPGHG+SVGSLG+Y  EK V  ++VK
Sbjct: 216 SSNVYFSRSHIATGDDCVSIGQGNSQITITSIKCGPGHGISVGSLGRYPNEKDVNGLVVK 275

Query: 286 NCTIFNATNGARIKTWA-SPVSGLASGITFEDIVMYNVKNPIIIDQTYGTKEKKASN--- 345
           +C I   TNG RIKTWA SP    A+ +TFE+I+M NV NPIIIDQ+Y       SN   
Sbjct: 276 DCKISGTTNGIRIKTWANSPGLSAATNMTFENIIMNNVTNPIIIDQSYCPFSSCISNVPS 335

Query: 346 -WKISDVHFKNIRGTSTTNVAVLLECSELLPCEGVELRDINL 347
             ++S+++FKNIRGTS++ VAV L CS  +PC+ V L +++L
Sbjct: 336 KVELSEIYFKNIRGTSSSLVAVQLHCSRGMPCKKVYLENVHL 373

[Close]

BLAST of Bhi09M000738 vs. TAIR10
Match: AT2G33160.1 (glycoside hydrolase family 28 protein / polygalacturonase (pectinase) family protein)

HSP 1 Score: 251.1 bits (640), Expect = 1.1e-66
Identity = 140/344 (40.70%), Postives = 193/344 (56.10%), Query Frame = 0


Query: 44  VFDVTKYGAKADGKTDDAQAFMTTWIAACRNTDGPAKFLIPQGTFLVGPVTFAGPCKSFP 103
           +FDV  YGA+AD + D+A AF   W  AC+ + G +   IP G F +  VTF+GPCKS  
Sbjct: 31  IFDVRNYGARADSQRDNAFAFTKAWNEACQWSYGRSTVYIPSGIFYLRQVTFSGPCKS-S 90

Query: 104 ITLENQGTVKATTDITEYSSPEWFSIEDITGFILTGSGVFDGQGTAAWPYNDCKTNNFCQ 163
           IT   +GT+ A  +    +  EW   + +    +TG G+ DGQG+ +WP NDC  N  C+
Sbjct: 91  ITFFIRGTLLAPRNPYAINQEEWILFKYVDNLTVTGGGLLDGQGSYSWPLNDCNKNTNCR 150

Query: 164 NLP-------IEKKK---------------------------NIIAPGNSPNTDGMHIST 223
            L        ++  K                            I APG+SPNTDG+ I  
Sbjct: 151 TLAMNIGFAFVKSSKINGLRSINSKMGHFNLFSVEDFNITGVTITAPGDSPNTDGIKIGK 210

Query: 224 SKLVTITNSVIGTGDDCVSIGHSTEKITVTGVTCGPGHGLSVGSLGKYSKEKSVYDVLVK 283
           S  + I N  IGTGDDC++I   T  + ++ V CGPGHG+SVGSLG+Y +EK+V  + V+
Sbjct: 211 SSHMQIYNVTIGTGDDCIAILDGTSNLDISDVRCGPGHGISVGSLGRYKEEKNVQGLTVR 270

Query: 284 NCTIFNATNGARIKTWASPVSGLA-SGITFEDIVMYNVKNPIIIDQTY------GTKEKK 343
           N  I   T+G RIKTWA  VS ++ S   +E+I M NV NPI+IDQ Y       +  K 
Sbjct: 271 NSIINGTTDGLRIKTWAKSVSQISVSNFLYENIQMINVGNPIVIDQQYCPHGQCDSPGKY 330

Query: 344 ASNWKISDVHFKNIRGTSTTNVAVLLECSELLPCEGVELRDINL 347
           AS+ +I DV + NI GTST+  A+ ++CS+  PC+ VEL +INL
Sbjct: 331 ASHVQIKDVKYNNIWGTSTSKEALKMQCSKTFPCQDVELSNINL 373

[Close]

BLAST of Bhi09M000738 vs. TAIR10
Match: AT2G15460.1 (Pectin lyase-like superfamily protein)

HSP 1 Score: 248.8 bits (634), Expect = 5.3e-66
Identity = 139/345 (40.29%), Postives = 192/345 (55.65%), Query Frame = 0


Query: 44  VFDVTKYGAKADGKTDDAQAFMTTWIAACRNTDGPAKFLIPQGTFLVGPVTFAGPCKSFP 103
           VF+V ++GAK DGKTD+A AF + W  AC+   G +K  +P+GTF +G V F GPCK+ P
Sbjct: 30  VFNVQRHGAKPDGKTDNANAFTSIWSRACKRISGSSKIYVPKGTFYLGGVEFVGPCKN-P 89

Query: 104 ITLENQGTVKATTDITEYSSPEWFSIEDITGFILTGSGVFDGQGTAAWPYNDCKTNNFCQ 163
           I     GT+ A  + ++     W +   I    ++GSG  DGQG  +WP+NDC TN  C 
Sbjct: 90  IEFIIDGTLLAPANPSDIKQDTWINFRYINNLSISGSGTLDGQGKQSWPHNDCHTNPNCP 149

Query: 164 NLPIE----------------------------------KKKNIIAPGNSPNTDGMHIST 223
            L +                                       I APG+SPNTDG+ + +
Sbjct: 150 KLAMTMGFAFVNNSNIKDITSLNSKMGHFNFFSVHHFNITGVTITAPGDSPNTDGIKMGS 209

Query: 224 SKLVTITNSVIGTGDDCVSIGHSTEKITVTGVTCGPGHGLSVGSLGKYSKEKSVYDVLVK 283
              + I+++ IGTGDDC++I   T  + ++ V CGPGHG+SVGSLGK   EK V D++V+
Sbjct: 210 CSNIQISDTNIGTGDDCIAILSGTTNLNISNVNCGPGHGISVGSLGKSKDEKDVKDLIVR 269

Query: 284 NCTIFNAT-NGARIKTWASPVSG-LASGITFEDIVMYNVKNPIIIDQTY------GTKEK 343
           +  IFN T +G RIKTW S  S  L S   +E+I M +V  PI IDQ Y        + K
Sbjct: 270 D-VIFNGTSDGIRIKTWESSASKILVSNFVYENIQMIDVGKPINIDQKYCPHPPCEHERK 329

Query: 344 KASNWKISDVHFKNIRGTSTTNVAVLLECSELLPCEGVELRDINL 347
             S+ +I ++  KNI GTS   VAV L+CS++ PC+ VEL DIN+
Sbjct: 330 GESHVQIQNLKLKNIYGTSKNKVAVNLQCSKIFPCKNVELIDINI 372

[Close]

BLAST of Bhi09M000738 vs. TAIR10
Match: AT2G15450.1 (Pectin lyase-like superfamily protein)

HSP 1 Score: 246.9 bits (629), Expect = 2.0e-65
Identity = 138/345 (40.00%), Postives = 191/345 (55.36%), Query Frame = 0


Query: 44  VFDVTKYGAKADGKTDDAQAFMTTWIAACRNTDGPAKFLIPQGTFLVGPVTFAGPCKSFP 103
           VF+V ++GAK DGKTD+A AF + W  AC+   G +K  +P+GTF +G V F GPCK+ P
Sbjct: 30  VFNVQRHGAKPDGKTDNANAFTSIWSRACKRISGSSKIYVPKGTFYLGGVEFVGPCKN-P 89

Query: 104 ITLENQGTVKATTDITEYSSPEWFSIEDITGFILTGSGVFDGQGTAAWPYNDCKTNNFCQ 163
           I     GT+ A  + ++     W +   I    ++GSG  DGQG  +WP+NDC TN  C 
Sbjct: 90  IEFIIDGTLLAPANPSDIKQDTWINFRYINNLSISGSGTLDGQGKQSWPHNDCHTNPNCP 149

Query: 164 NLPIE----------------------------------KKKNIIAPGNSPNTDGMHIST 223
            L +                                       I APG+SPNTDG+ + +
Sbjct: 150 KLAMTMGFAFVNNSNIKDITSLNSKMGHFNFFSVHHFNITGVTITAPGDSPNTDGIKMGS 209

Query: 224 SKLVTITNSVIGTGDDCVSIGHSTEKITVTGVTCGPGHGLSVGSLGKYSKEKSVYDVLVK 283
              + I+++ IGTGDDC++I   T  + ++ V CGPGHG+SVGSLGK   EK V D++V+
Sbjct: 210 CSNIQISDTNIGTGDDCIAILSGTTNLNISNVNCGPGHGISVGSLGKNKDEKDVKDLIVR 269

Query: 284 NCTIFNAT-NGARIKTWASPVSG-LASGITFEDIVMYNVKNPIIIDQTY------GTKEK 343
           +  IFN T +G RIK W S  S  L S   +E+I M +V  PI IDQ Y        + K
Sbjct: 270 D-VIFNGTSDGIRIKNWESSASKILVSNFVYENIQMIDVGKPINIDQKYCPHPPCEHERK 329

Query: 344 KASNWKISDVHFKNIRGTSTTNVAVLLECSELLPCEGVELRDINL 347
             S+ +I ++  KNI GTS   VAV L+CS++ PC+ VEL DIN+
Sbjct: 330 GESHVQIQNLKLKNIYGTSKNKVAVNLQCSKIFPCKNVELIDINI 372

[Close]

BLAST of Bhi09M000738 vs. TrEMBL
Match: tr|A0A0A0KMA6|A0A0A0KMA6_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G222970 PE=3 SV=1)

HSP 1 Score: 595.9 bits (1535), Expect = 6.5e-167
Identity = 298/381 (78.22%), Postives = 313/381 (82.15%), Query Frame = 0


Query: 3   NQKLLHPAAAPRSLGIGTLPDIGGTVNDIKANADLNGNGGAVFDVTKYGAKADGKTDDAQ 62
           N++ L PA APR LG  TLPDIGGTVNDI AN DLN NGG+VFDVTK+GAKADGKTDDAQ
Sbjct: 14  NKQFLRPAEAPRHLGFNTLPDIGGTVNDINANVDLNENGGSVFDVTKHGAKADGKTDDAQ 73

Query: 63  AFMTTWIAACRNTDGPAKFLIPQGTFLVGPVTFAGPCKSFPITLENQGTVKATTDITEYS 122
           AFMTTWIAACRNT GPAKFLIPQGTFLVGPVTFAGPCKS+PITLENQGTVKATTDI+ YS
Sbjct: 74  AFMTTWIAACRNTVGPAKFLIPQGTFLVGPVTFAGPCKSYPITLENQGTVKATTDISAYS 133

Query: 123 SPEWFSIEDITGFILTGSGVFDGQGTAAWPYNDCKTNNFCQNLPIEKK------------ 182
           SPEWFS+EDITGFILTGSGVFDGQG + WPYNDCK NN CQ LPI  K            
Sbjct: 134 SPEWFSLEDITGFILTGSGVFDGQGLSVWPYNDCKKNNLCQLLPISIKFTRLNHTIVDGL 193

Query: 183 ----------------------KNIIAPGNSPNTDGMHISTSKLVTITNSVIGTGDDCVS 242
                                   IIAP NSPNTDGMH+STSKLVTI NS+IGTGDDCVS
Sbjct: 194 TSINSMGFHTSVFYCYNFTATNMKIIAPHNSPNTDGMHLSTSKLVTIANSIIGTGDDCVS 253

Query: 243 IGHSTEKITVTGVTCGPGHGLSVGSLGKYSKEKSVYDVLVKNCTIFNATNGARIKTWASP 302
           IGHSTE ITVT VTCGPGHGL   SLGKYSKEK VYDVLVKNCTIFNATNGARIKTWASP
Sbjct: 254 IGHSTENITVTNVTCGPGHGL---SLGKYSKEKGVYDVLVKNCTIFNATNGARIKTWASP 313

Query: 303 VSGLASGITFEDIVMYNVKNPIIIDQTYGTKEKKASNWKISDVHFKNIRGTSTTNVAVLL 350
           VSGLAS I FEDIVMYNVKNPIIIDQTYGTK+KK SNWK+S+V FKNIRGTSTTNVAVLL
Sbjct: 314 VSGLASRIIFEDIVMYNVKNPIIIDQTYGTKKKKESNWKVSNVQFKNIRGTSTTNVAVLL 373

[Close]

BLAST of Bhi09M000738 vs. TrEMBL
Match: tr|A0A1S3BRD3|A0A1S3BRD3_CUCME (exopolygalacturonase-like OS=Cucumis melo OX=3656 GN=LOC103492867 PE=3 SV=1)

HSP 1 Score: 570.1 bits (1468), Expect = 3.8e-159
Identity = 286/383 (74.67%), Postives = 308/383 (80.42%), Query Frame = 0


Query: 2   VNQKLLHPAAAPRSLGIGTLPDIGGTVN-DIKANADLNGNGGAVFDVTKYGAKADGKTDD 61
           +NQ+L  P AAP  LGI TLPD+   VN DI  N DLNGNGG+VF VTK+GAKADGKTDD
Sbjct: 43  INQQLPSPVAAPGPLGIETLPDLDNMVNDDINTNNDLNGNGGSVFHVTKHGAKADGKTDD 102

Query: 62  AQAFMTTWIAACRNTDGPAKFLIPQGTFLVGPVTFAGPCKSFPITLENQGTVKATTDITE 121
           AQAF+TTWI ACRNT GPAK LIP+GT+LVGPVT AGPCKSFPITLENQGTVKATTDI++
Sbjct: 103 AQAFVTTWIEACRNTVGPAKILIPEGTYLVGPVTLAGPCKSFPITLENQGTVKATTDISQ 162

Query: 122 YSSPEWFSIEDITGFILTGSGVFDGQGTAAWPYNDCKTNNFCQNLPIEKK---------- 181
           YSSPEWFSIEDITGFILTGSGVFDGQG AAWPYNDCK NN CQ LPI  K          
Sbjct: 163 YSSPEWFSIEDITGFILTGSGVFDGQGVAAWPYNDCKNNNICQLLPISIKFSRLNDTIVD 222

Query: 182 ------------------------KNIIAPGNSPNTDGMHISTSKLVTITNSVIGTGDDC 241
                                    NIIAP +SPNTDG+H+STSKLV I NS+IGTGDDC
Sbjct: 223 RLTSLNSKGFHMSLFSCYNFTATNINIIAPHDSPNTDGIHLSTSKLVNIANSIIGTGDDC 282

Query: 242 VSIGHSTEKITVTGVTCGPGHGLSVGSLGKYSKEKSVYDVLVKNCTIFNATNGARIKTWA 301
           VSIGHSTEKITVT VTCGPGHGLSVGSLGKY +EK VYDVLVKNCTIFNATNGARIKT+A
Sbjct: 283 VSIGHSTEKITVTNVTCGPGHGLSVGSLGKYPEEKDVYDVLVKNCTIFNATNGARIKTFA 342

Query: 302 SPVSGLASGITFEDIVMYNVKNPIIIDQTYGTKEKKASNWKISDVHFKNIRGTSTTNVAV 350
           SP+SG ASGI FEDIVMYNVK PIIIDQTY T E K S WK+SDVHFKNIRGTS TNVAV
Sbjct: 343 SPISGSASGIIFEDIVMYNVKYPIIIDQTYDTNENKESKWKVSDVHFKNIRGTSATNVAV 402

[Close]

BLAST of Bhi09M000738 vs. TrEMBL
Match: tr|A0A0A0K5G0|A0A0A0K5G0_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G375750 PE=3 SV=1)

HSP 1 Score: 530.8 bits (1366), Expect = 2.6e-147
Identity = 269/384 (70.05%), Postives = 295/384 (76.82%), Query Frame = 0


Query: 2   VNQKLL-HPAAAPRSLGIGTLPDIGGT-VNDIKANADLNGNGGAVFDVTKYGAKADGKTD 61
           +NQ+L+  P A P  LG+ TLPD+  T             NG +VFDVTK+GAKADGKTD
Sbjct: 44  INQQLIPSPTATPGPLGMETLPDLDDTXXXXXXXXXXXXXNGESVFDVTKHGAKADGKTD 103

Query: 62  DAQAFMTTWIAACRNTDGPAKFLIPQGTFLVGPVTFAGPCKSFPITLENQGTVKATTDIT 121
           DAQAF TTWIAACRNT GP K LIP+GT+LVGP+T AGPCKSFPIT+ENQG VKATTD++
Sbjct: 104 DAQAFETTWIAACRNTVGPVKILIPKGTYLVGPMTLAGPCKSFPITIENQGIVKATTDLS 163

Query: 122 EYSSPEWFSIEDITGFILTGSGVFDGQGTAAWPYNDCKTNNFCQNLPIEKK--------- 181
           EYSSPEWFSIEDITGFILTGSGVFDGQGTA W YNDCK+N  CQ LPI  K         
Sbjct: 164 EYSSPEWFSIEDITGFILTGSGVFDGQGTAVWAYNDCKSNKDCQLLPISIKFSGLNHTIV 223

Query: 182 -------------------------KNIIAPGNSPNTDGMHISTSKLVTITNSVIGTGDD 241
                                     NIIAP  SPNTDG+H+STS+LV I NS+IGTGDD
Sbjct: 224 DGITSLNSKGFHMSLFNCYNFTITNVNIIAPDESPNTDGIHLSTSELVNIMNSIIGTGDD 283

Query: 242 CVSIGHSTEKITVTGVTCGPGHGLSVGSLGKYSKEKSVYDVLVKNCTIFNATNGARIKTW 301
           CVSIGHST KITVT VTCGPGHGLSVGSLGKYS+EK VYDVLVKNCTIFNATNGARIKT+
Sbjct: 284 CVSIGHSTVKITVTNVTCGPGHGLSVGSLGKYSREKDVYDVLVKNCTIFNATNGARIKTF 343

Query: 302 ASPVSGLASGITFEDIVMYNVKNPIIIDQTYGTKEKKASNWKISDVHFKNIRGTSTTNVA 350
           ASP+SGLASGI FEDI+MYNVK PIIIDQTY T E K S WK+SDVHFKNIRGTS TNV 
Sbjct: 344 ASPISGLASGIIFEDIIMYNVKYPIIIDQTYSTDENKESKWKVSDVHFKNIRGTSATNVG 403

[Close]

BLAST of Bhi09M000738 vs. TrEMBL
Match: tr|A0A0A0K5I2|A0A0A0K5I2_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G398220 PE=3 SV=1)

HSP 1 Score: 443.0 bits (1138), Expect = 7.0e-121
Identity = 228/304 (75.00%), Postives = 247/304 (81.25%), Query Frame = 0


Query: 2   VNQKLLHPAAAPRSLGIGTLPDI-GGTVNDIKANADLNGNGGAVFDVTKYGAKADGKTDD 61
           VN++ L PA AP  LG  TLP+I GGTVNDIK N DLN NGG VFDVTK+GAKADG+TDD
Sbjct: 44  VNEQFLRPAEAPWHLGFSTLPNIGGGTVNDIKVNVDLNENGGLVFDVTKHGAKADGETDD 103

Query: 62  AQAFMTTWIAACRNTDGPAKFLIPQGTFLVGPVTFAGPCKSFPITLENQGTVKATTDITE 121
           AQAFMTTWIAACRNT GPAKFLIPQ  FLVGPVTFAGPCKSFPITLENQGTVKATTDI+ 
Sbjct: 104 AQAFMTTWIAACRNTVGPAKFLIPQDIFLVGPVTFAGPCKSFPITLENQGTVKATTDISA 163

Query: 122 YSSPEWFSIEDITGFILTGSGVFDGQGTAAWPYN-------DCKTN----------NFCQ 181
           YSS EWFSIED+TGFILTGSGVFDGQG ++  ++       D  T+           +C 
Sbjct: 164 YSSSEWFSIEDVTGFILTGSGVFDGQGVSSIKFSRLNHTIIDGLTSINSMGFHTSVFYCY 223

Query: 182 NLPIEKKKNIIAPGNSPNTDGMHISTSKLVTITNSVIGTGDDCVSIGHSTEKITVTGVTC 241
           N      K I AP NSPNTDGMH+STSKLVTI+NS+IGTGDDCVSIGHSTE I +T VTC
Sbjct: 224 NFTATNMK-ITAPHNSPNTDGMHLSTSKLVTISNSIIGTGDDCVSIGHSTENIIITNVTC 283

Query: 242 GPGHGLSVGSLGKYSKEKSVYDVLVKNCTIFNATNGARIKTWASPVSGLASGITFEDIVM 288
           GPGHGLSVGSLGKYSKEK VYDVLVKNCTIFNATNGARIKT+ASP+ GLAS I FEDIVM
Sbjct: 284 GPGHGLSVGSLGKYSKEKGVYDVLVKNCTIFNATNGARIKTFASPIPGLASRIVFEDIVM 343

[Close]

BLAST of Bhi09M000738 vs. TrEMBL
Match: tr|W9QFJ0|W9QFJ0_9ROSA (Exopolygalacturonase clone GBGE184 OS=Morus notabilis OX=981085 GN=L484_011125 PE=3 SV=1)

HSP 1 Score: 380.6 bits (976), Expect = 4.3e-102
Identity = 193/361 (53.46%), Postives = 236/361 (65.37%), Query Frame = 0


Query: 25  GGTVNDIKANADLNGNGGAVFDVTKYGAKADG-KTDDAQAFMTTWIAACRNTDGPAKFLI 84
           G   +D      L   G  +FDVTKYGA A+  + D+ QAFM TW+AACRN++ PAK +I
Sbjct: 34  GHVSDDTGLRRGLTETGRQIFDVTKYGAVANNERKDNVQAFMKTWVAACRNSNAPAKIVI 93

Query: 85  PQGTFLVGPVTFAGPCKSF-PITLENQGTVKATTDITEYSSPEWFSIEDITGFILTGSGV 144
           P G FLVGPV F GPCKS  PI +E QGTVKATTD+++YSSPEWFS E + G +LTG G 
Sbjct: 94  PTGAFLVGPVVFQGPCKSSEPIVIEVQGTVKATTDVSDYSSPEWFSFEFVNGLVLTGKGT 153

Query: 145 FDGQGTAAWPYNDCKTNNFCQNLP---------------------------IEKKKN--- 204
           FDGQG   W YNDCKTN  CQ+L                            I + KN   
Sbjct: 154 FDGQGPKVWHYNDCKTNPNCQHLASSLRFSNVNNAYVEGITSLNSKWFHFFIYESKNITL 213

Query: 205 ----IIAPGNSPNTDGMHISTSKLVTITNSVIGTGDDCVSIGHSTEKITVTGVTCGPGHG 264
               I AP  SPNTDG+HIS S  V +TN+ I TGDDC+ I      I +T VTCGPGHG
Sbjct: 214 NNVKITAPAESPNTDGIHISASTSVNVTNTAIATGDDCIGIVQDCHNIYITNVTCGPGHG 273

Query: 265 LSVGSLGKYSKEKSVYDVLVKNCTIFNATNGARIKTWASPVSGLASGITFEDIVMYNVKN 324
           +SVGSLGK+S +K V +++V+NCT+ N TNGARIKTW    SG A+GI +E+I+M NVKN
Sbjct: 274 ISVGSLGKWSYDKGVSNIVVRNCTLVNTTNGARIKTWGGTTSGEATGIIYENIIMKNVKN 333

Query: 325 PIIIDQTYGTKEKKASNWKISDVHFKNIRGTSTTNVAVLLECSELLPCEGVELRDINLLW 350
           PIIIDQ YG K  + S WKI DVHF+NI+GTS + V++ L CS L PCE VE+ +INL +
Sbjct: 334 PIIIDQNYGRK-NQPSKWKIKDVHFRNIKGTSASKVSISLGCSSLFPCEVVEMSNINLSY 393

[Close]

BLAST of Bhi09M000738 vs. NCBI nr
Match: KGN50738.1 (hypothetical protein Csa_5G222970 [Cucumis sativus])

HSP 1 Score: 595.9 bits (1535), Expect = 9.8e-167
Identity = 298/381 (78.22%), Postives = 313/381 (82.15%), Query Frame = 0


Query: 3   NQKLLHPAAAPRSLGIGTLPDIGGTVNDIKANADLNGNGGAVFDVTKYGAKADGKTDDAQ 62
           N++ L PA APR LG  TLPDIGGTVNDI AN DLN NGG+VFDVTK+GAKADGKTDDAQ
Sbjct: 14  NKQFLRPAEAPRHLGFNTLPDIGGTVNDINANVDLNENGGSVFDVTKHGAKADGKTDDAQ 73

Query: 63  AFMTTWIAACRNTDGPAKFLIPQGTFLVGPVTFAGPCKSFPITLENQGTVKATTDITEYS 122
           AFMTTWIAACRNT GPAKFLIPQGTFLVGPVTFAGPCKS+PITLENQGTVKATTDI+ YS
Sbjct: 74  AFMTTWIAACRNTVGPAKFLIPQGTFLVGPVTFAGPCKSYPITLENQGTVKATTDISAYS 133

Query: 123 SPEWFSIEDITGFILTGSGVFDGQGTAAWPYNDCKTNNFCQNLPIEKK------------ 182
           SPEWFS+EDITGFILTGSGVFDGQG + WPYNDCK NN CQ LPI  K            
Sbjct: 134 SPEWFSLEDITGFILTGSGVFDGQGLSVWPYNDCKKNNLCQLLPISIKFTRLNHTIVDGL 193

Query: 183 ----------------------KNIIAPGNSPNTDGMHISTSKLVTITNSVIGTGDDCVS 242
                                   IIAP NSPNTDGMH+STSKLVTI NS+IGTGDDCVS
Sbjct: 194 TSINSMGFHTSVFYCYNFTATNMKIIAPHNSPNTDGMHLSTSKLVTIANSIIGTGDDCVS 253

Query: 243 IGHSTEKITVTGVTCGPGHGLSVGSLGKYSKEKSVYDVLVKNCTIFNATNGARIKTWASP 302
           IGHSTE ITVT VTCGPGHGL   SLGKYSKEK VYDVLVKNCTIFNATNGARIKTWASP
Sbjct: 254 IGHSTENITVTNVTCGPGHGL---SLGKYSKEKGVYDVLVKNCTIFNATNGARIKTWASP 313

Query: 303 VSGLASGITFEDIVMYNVKNPIIIDQTYGTKEKKASNWKISDVHFKNIRGTSTTNVAVLL 350
           VSGLAS I FEDIVMYNVKNPIIIDQTYGTK+KK SNWK+S+V FKNIRGTSTTNVAVLL
Sbjct: 314 VSGLASRIIFEDIVMYNVKNPIIIDQTYGTKKKKESNWKVSNVQFKNIRGTSTTNVAVLL 373

[Close]

BLAST of Bhi09M000738 vs. NCBI nr
Match: XP_011656188.1 (PREDICTED: exopolygalacturonase-like [Cucumis sativus])

HSP 1 Score: 595.9 bits (1535), Expect = 9.8e-167
Identity = 298/381 (78.22%), Postives = 313/381 (82.15%), Query Frame = 0


Query: 3   NQKLLHPAAAPRSLGIGTLPDIGGTVNDIKANADLNGNGGAVFDVTKYGAKADGKTDDAQ 62
           N++ L PA APR LG  TLPDIGGTVNDI AN DLN NGG+VFDVTK+GAKADGKTDDAQ
Sbjct: 42  NKQFLRPAEAPRHLGFNTLPDIGGTVNDINANVDLNENGGSVFDVTKHGAKADGKTDDAQ 101

Query: 63  AFMTTWIAACRNTDGPAKFLIPQGTFLVGPVTFAGPCKSFPITLENQGTVKATTDITEYS 122
           AFMTTWIAACRNT GPAKFLIPQGTFLVGPVTFAGPCKS+PITLENQGTVKATTDI+ YS
Sbjct: 102 AFMTTWIAACRNTVGPAKFLIPQGTFLVGPVTFAGPCKSYPITLENQGTVKATTDISAYS 161

Query: 123 SPEWFSIEDITGFILTGSGVFDGQGTAAWPYNDCKTNNFCQNLPIEKK------------ 182
           SPEWFS+EDITGFILTGSGVFDGQG + WPYNDCK NN CQ LPI  K            
Sbjct: 162 SPEWFSLEDITGFILTGSGVFDGQGLSVWPYNDCKKNNLCQLLPISIKFTRLNHTIVDGL 221

Query: 183 ----------------------KNIIAPGNSPNTDGMHISTSKLVTITNSVIGTGDDCVS 242
                                   IIAP NSPNTDGMH+STSKLVTI NS+IGTGDDCVS
Sbjct: 222 TSINSMGFHTSVFYCYNFTATNMKIIAPHNSPNTDGMHLSTSKLVTIANSIIGTGDDCVS 281

Query: 243 IGHSTEKITVTGVTCGPGHGLSVGSLGKYSKEKSVYDVLVKNCTIFNATNGARIKTWASP 302
           IGHSTE ITVT VTCGPGHGL   SLGKYSKEK VYDVLVKNCTIFNATNGARIKTWASP
Sbjct: 282 IGHSTENITVTNVTCGPGHGL---SLGKYSKEKGVYDVLVKNCTIFNATNGARIKTWASP 341

Query: 303 VSGLASGITFEDIVMYNVKNPIIIDQTYGTKEKKASNWKISDVHFKNIRGTSTTNVAVLL 350
           VSGLAS I FEDIVMYNVKNPIIIDQTYGTK+KK SNWK+S+V FKNIRGTSTTNVAVLL
Sbjct: 342 VSGLASRIIFEDIVMYNVKNPIIIDQTYGTKKKKESNWKVSNVQFKNIRGTSTTNVAVLL 401

[Close]

BLAST of Bhi09M000738 vs. NCBI nr
Match: XP_008451635.1 (PREDICTED: exopolygalacturonase-like [Cucumis melo])

HSP 1 Score: 570.1 bits (1468), Expect = 5.8e-159
Identity = 286/383 (74.67%), Postives = 308/383 (80.42%), Query Frame = 0


Query: 2   VNQKLLHPAAAPRSLGIGTLPDIGGTVN-DIKANADLNGNGGAVFDVTKYGAKADGKTDD 61
           +NQ+L  P AAP  LGI TLPD+   VN DI  N DLNGNGG+VF VTK+GAKADGKTDD
Sbjct: 43  INQQLPSPVAAPGPLGIETLPDLDNMVNDDINTNNDLNGNGGSVFHVTKHGAKADGKTDD 102

Query: 62  AQAFMTTWIAACRNTDGPAKFLIPQGTFLVGPVTFAGPCKSFPITLENQGTVKATTDITE 121
           AQAF+TTWI ACRNT GPAK LIP+GT+LVGPVT AGPCKSFPITLENQGTVKATTDI++
Sbjct: 103 AQAFVTTWIEACRNTVGPAKILIPEGTYLVGPVTLAGPCKSFPITLENQGTVKATTDISQ 162

Query: 122 YSSPEWFSIEDITGFILTGSGVFDGQGTAAWPYNDCKTNNFCQNLPIEKK---------- 181
           YSSPEWFSIEDITGFILTGSGVFDGQG AAWPYNDCK NN CQ LPI  K          
Sbjct: 163 YSSPEWFSIEDITGFILTGSGVFDGQGVAAWPYNDCKNNNICQLLPISIKFSRLNDTIVD 222

Query: 182 ------------------------KNIIAPGNSPNTDGMHISTSKLVTITNSVIGTGDDC 241
                                    NIIAP +SPNTDG+H+STSKLV I NS+IGTGDDC
Sbjct: 223 RLTSLNSKGFHMSLFSCYNFTATNINIIAPHDSPNTDGIHLSTSKLVNIANSIIGTGDDC 282

Query: 242 VSIGHSTEKITVTGVTCGPGHGLSVGSLGKYSKEKSVYDVLVKNCTIFNATNGARIKTWA 301
           VSIGHSTEKITVT VTCGPGHGLSVGSLGKY +EK VYDVLVKNCTIFNATNGARIKT+A
Sbjct: 283 VSIGHSTEKITVTNVTCGPGHGLSVGSLGKYPEEKDVYDVLVKNCTIFNATNGARIKTFA 342

Query: 302 SPVSGLASGITFEDIVMYNVKNPIIIDQTYGTKEKKASNWKISDVHFKNIRGTSTTNVAV 350
           SP+SG ASGI FEDIVMYNVK PIIIDQTY T E K S WK+SDVHFKNIRGTS TNVAV
Sbjct: 343 SPISGSASGIIFEDIVMYNVKYPIIIDQTYDTNENKESKWKVSDVHFKNIRGTSATNVAV 402

[Close]

BLAST of Bhi09M000738 vs. NCBI nr
Match: XP_023549400.1 (exopolygalacturonase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 536.6 bits (1381), Expect = 7.1e-149
Identity = 274/379 (72.30%), Postives = 297/379 (78.36%), Query Frame = 0


Query: 9   PAAAPRSLGIGTLPDIGG--TVNDIKANADL--NGNGGAVFDVTKYGAKADGKTDDAQAF 68
           P A P  LGIGTL +  G   +N  KA   L  NGNG AVFDV KYGAKA+GKTDDAQAF
Sbjct: 52  PPAPPLPLGIGTLANSRGRVAINGTKAAVYLKGNGNGNAVFDVKKYGAKANGKTDDAQAF 111

Query: 69  MTTWIAACRNTDGPAKFLIPQGTFLVGPVTFAGPCKSFPITLENQGTVKATTDITEYSSP 128
           MTTWIAACRNT GPAKFLIP+GTFLVGPV FAGPCKS+PIT+E QGTVKATTDI+EYSSP
Sbjct: 112 MTTWIAACRNTTGPAKFLIPKGTFLVGPVVFAGPCKSWPITIEIQGTVKATTDISEYSSP 171

Query: 129 EWFSIEDITGFILTGSGVFDGQGTAAWPYNDCKTNNFCQNLPIEKK-------------- 188
           +W SIE ITG ILTGSGVFDGQG +AWPYNDCKTNN CQ LP   K              
Sbjct: 172 DWISIEGITGLILTGSGVFDGQGASAWPYNDCKTNNNCQILPNSIKFTRLNHSIVDGLTS 231

Query: 189 --------------------KNIIAPGNSPNTDGMHISTSKLVTITNSVIGTGDDCVSIG 248
                                NIIAPGNSPNTDGMH+STSKLVTI++S IGTGDDCVSIG
Sbjct: 232 INSKGFHTSVFRCYNFTATNMNIIAPGNSPNTDGMHLSTSKLVTISSSTIGTGDDCVSIG 291

Query: 249 HSTEKITVTGVTCGPGHGLSVGSLGKYSKEKSVYDVLVKNCTIFNATNGARIKTWASPVS 308
           HS EKITVT +TCGPGHG+S+GSLG+Y  EKSV DVLV+NCTIFNATNGARIKTWA  +S
Sbjct: 292 HSCEKITVTNITCGPGHGISIGSLGRYKTEKSVSDVLVQNCTIFNATNGARIKTWADTIS 351

Query: 309 GLASGITFEDIVMYNVKNPIIIDQTYGTKEKKASNWKISDVHFKNIRGTSTTNVAVLLEC 350
           G A GI F++IVM  VKNPIIIDQTYGTK+KKAS WKIS+VHFKNIRGTSTTNVAVLLEC
Sbjct: 352 GSAVGIIFDNIVMRKVKNPIIIDQTYGTKKKKASKWKISNVHFKNIRGTSTTNVAVLLEC 411

[Close]

BLAST of Bhi09M000738 vs. NCBI nr
Match: XP_022991773.1 (exopolygalacturonase-like [Cucurbita maxima])

HSP 1 Score: 535.0 bits (1377), Expect = 2.1e-148
Identity = 273/377 (72.41%), Postives = 295/377 (78.25%), Query Frame = 0


Query: 9   PAAAPRSLGIGTLPDIGGTV--NDIKANADLNGNGGAVFDVTKYGAKADGKTDDAQAFMT 68
           P AAP  LGI T  +  G+V  N  KA   L GNG AVFDV KYGAKA+GK+DDAQAFMT
Sbjct: 52  PPAAPLPLGIRTPANRRGSVAINGTKAAVYLKGNGNAVFDVKKYGAKANGKSDDAQAFMT 111

Query: 69  TWIAACRNTDGPAKFLIPQGTFLVGPVTFAGPCKSFPITLENQGTVKATTDITEYSSPEW 128
           TWIAACRNT GPAKFLIP+G FLVGPVTFAGPCKS PIT+E QGTVKATTDI+EYSSP+W
Sbjct: 112 TWIAACRNTTGPAKFLIPKGIFLVGPVTFAGPCKSMPITIEIQGTVKATTDISEYSSPDW 171

Query: 129 FSIEDITGFILTGSGVFDGQGTAAWPYNDCKTNNFCQNLPIEKK---------------- 188
            SIE ITG ILTGSGVFDGQG +AWPYNDCK NN CQ LP   K                
Sbjct: 172 ISIEGITGLILTGSGVFDGQGASAWPYNDCKINNNCQILPNSIKFTRLNHSIVDGLTSIN 231

Query: 189 ------------------KNIIAPGNSPNTDGMHISTSKLVTITNSVIGTGDDCVSIGHS 248
                              NI+APGNSPNTDGMH+STSKLVTI++S IGTGDDCVSIGHS
Sbjct: 232 SKGFHTSVFRCYNFTATNMNIMAPGNSPNTDGMHLSTSKLVTISSSTIGTGDDCVSIGHS 291

Query: 249 TEKITVTGVTCGPGHGLSVGSLGKYSKEKSVYDVLVKNCTIFNATNGARIKTWASPVSGL 308
            EKITVT VTCGPGHG+S+GSLG+Y  EKSV DVLV+NCTIFNATNGARIKTWA+ +SG 
Sbjct: 292 CEKITVTNVTCGPGHGISIGSLGRYKTEKSVLDVLVQNCTIFNATNGARIKTWANTISGS 351

Query: 309 ASGITFEDIVMYNVKNPIIIDQTYGTKEKKASNWKISDVHFKNIRGTSTTNVAVLLECSE 350
           A GI F++IVM  VKNPIIIDQTYGTKEKKAS WKISDVHFKNIRGTSTTNVAVLLECSE
Sbjct: 352 AVGIVFDNIVMRKVKNPIIIDQTYGTKEKKASKWKISDVHFKNIRGTSTTNVAVLLECSE 411

The following BLAST results are available for this feature:

BLAST of Bhi09M000738 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|P49062\|PGLR1_ARATH	4.6e-75	44.77	Exopolygalacturonase clone GBGE184 OS=Arabidopsis thaliana OX=3702 GN=PGA3 PE=2 ...	[more]
sp\|P35339\|PGLR3_MAIZE	3.6e-72	43.30	Exopolygalacturonase OS=Zea mays OX=4577 GN=PG2C PE=2 SV=1	[more]
sp\|Q6H9K0\|PGLR2_PLAAC	8.1e-72	42.03	Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 S...	[more]
sp\|Q39766\|PGLR_GOSBA	3.4e-70	41.59	Polygalacturonase OS=Gossypium barbadense OX=3634 GN=G9 PE=2 SV=1	[more]
sp\|Q39786\|PGLR_GOSHI	3.4e-70	41.59	Polygalacturonase OS=Gossypium hirsutum OX=3635 GN=G9 PE=2 SV=1	[more]

BLAST of Bhi09M000738 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G02790.1	2.6e-76	44.77	polygalacturonase 4	[more]
AT4G18180.1	5.0e-72	42.98	Pectin lyase-like superfamily protein	[more]
AT2G33160.1	1.1e-66	40.70	glycoside hydrolase family 28 protein / polygalacturonase (pectinase) family pro...	[more]
AT2G15460.1	5.3e-66	40.29	Pectin lyase-like superfamily protein	[more]
AT2G15450.1	2.0e-65	40.00	Pectin lyase-like superfamily protein	[more]

BLAST of Bhi09M000738 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0KMA6\|A0A0A0KMA6_CUCSA	6.5e-167	78.22	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G222970 PE=3 SV=1	[more]
tr\|A0A1S3BRD3\|A0A1S3BRD3_CUCME	3.8e-159	74.67	exopolygalacturonase-like OS=Cucumis melo OX=3656 GN=LOC103492867 PE=3 SV=1	[more]
tr\|A0A0A0K5G0\|A0A0A0K5G0_CUCSA	2.6e-147	70.05	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G375750 PE=3 SV=1	[more]
tr\|A0A0A0K5I2\|A0A0A0K5I2_CUCSA	7.0e-121	75.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G398220 PE=3 SV=1	[more]
tr\|W9QFJ0\|W9QFJ0_9ROSA	4.3e-102	53.46	Exopolygalacturonase clone GBGE184 OS=Morus notabilis OX=981085 GN=L484_011125 P...	[more]

BLAST of Bhi09M000738 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN50738.1	9.8e-167	78.22	hypothetical protein Csa_5G222970 [Cucumis sativus]	[more]
XP_011656188.1	9.8e-167	78.22	PREDICTED: exopolygalacturonase-like [Cucumis sativus]	[more]
XP_008451635.1	5.8e-159	74.67	PREDICTED: exopolygalacturonase-like [Cucumis melo]	[more]
XP_023549400.1	7.1e-149	72.30	exopolygalacturonase-like [Cucurbita pepo subsp. pepo]	[more]
XP_022991773.1	2.1e-148	72.41	exopolygalacturonase-like [Cucurbita maxima]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004650	polygalacturonase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0005975	carbohydrate metabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR011050	Pectin_lyase_fold/virulence
IPR000743	Glyco_hydro_28
IPR012334	Pectin_lyas_fold
IPR006626	PbH1

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
biological_process	GO:0071555	cell wall organization
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0004650	polygalacturonase activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Bhi09G000738	Bhi09G000738	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Bhi09M000738	Bhi09M000738-protein	polypeptide

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Bhi09M000738.exon5	Bhi09M000738.exon5	exon
Bhi09M000738.exon4	Bhi09M000738.exon4	exon
Bhi09M000738.exon3	Bhi09M000738.exon3	exon
Bhi09M000738.exon2	Bhi09M000738.exon2	exon
Bhi09M000738.exon1	Bhi09M000738.exon1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.Bhi09M000738	cds.Bhi09M000738	CDS

InterPro

Analysis Name: InterPro Annotations of wax gourd
Date Performed: 2019-11-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006626	Parallel beta-helix repeat	SMART	SM00710	pbh1	coord: 190..211 e-value: 750.0 score: 8.1 coord: 273..294 e-value: 1100.0 score: 7.1 coord: 303..330 e-value: 5300.0 score: 2.0 coord: 243..264 e-value: 10.0 score: 15.1
IPR012334	Pectin lyase fold	GENE3D	G3DSA:2.160.20.10		coord: 167..358 e-value: 1.2E-64 score: 220.4
IPR012334	Pectin lyase fold	GENE3D	G3DSA:2.160.20.10		coord: 34..166 e-value: 2.0E-27 score: 98.2
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 172..351 e-value: 1.4E-60 score: 205.0 coord: 77..166 e-value: 5.8E-13 score: 48.5
IPR000743	Glycoside hydrolase, family 28	PROSITE	PS00502	POLYGALACTURONASE	coord: 220..233
None	No IPR available	PANTHER	PTHR31375:SF17	EXOPOLYGALACTURONASE CLONE GBGE184	coord: 27..167
None	No IPR available	PANTHER	PTHR31375:SF17	EXOPOLYGALACTURONASE CLONE GBGE184	coord: 171..351
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 171..351
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 27..167
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	SSF51126	Pectin lyase-like	coord: 43..350