Bhi08M001211 (mRNA) Wax gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Bhi08M001211
Type	mRNA
Organism	Benincasa hispida (Wax gourd)
Description	Pectinesterase
Location	chr8 : 42876438 .. 42877502 (-)
Sequence length	966

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAACTTTGTTGATAGCTTTACTTTTAACACTGTTGTCTCTTTAGATGGAACTGGAAATTTTAGAAGCATTAATGCAGCTATAGCAGCTGCACCAAGTAATAGAAAATCTCGATTTTACATTCATGTGAAACCTGGGATTTACAAAGAACATGTTGAAATCGAAAAGAAAAAGACTTTCATTGCGTTAATCGGTGACAACGCTGCAAACACCATCATTGTTGATAGTAGAAGCCATGCTACTGGTTTTAACACCTTCCAATCCGCTACATTCGGTAAGTTTGAACTTGCCACTTTTTGTTTCCATTGCTTTGCTTTCAAATATTAACAGTGCATATTTGATGTTGAGTTATCCCTAAACTTTTCTTTTGTAGTTGTCAAGGGTGATAACTTTATGGCACAGTCCATAACGTTTGAAAATTCTGCTGGACCTGAAAATGGTCAAGCTGTTGCCCTTCTCAACAACCGCGCCAATCATACGGTATACTACAAATGTGTATTTCTTGGCTATCAAGACACTCTTTATGTCAATGGAGACAATGTATTCTTCAAAGATAGTGAAATTTACGGCACGGTAGATTTCATATTTGGATATGGACAAGCTGTGTATCAAGATTGTAATATATATGCAAGATTAGGCAATAGAACGATTACAATCACAGCACAATCTAAACAATTATGGAACCAAAGAAGTGGCTTCGTTTTCCAAAATTGCAGTGTAATGGCCTCTCCTGAACTCAGGTCGATAGAAAATCTAAATTTTGAAGTTTATCTTGGTCGGCCTTGGAGAGACTATTCGACTGTAATTTTTATGGACTCATTTTTGGACTATCTAGTTAATCCAGCAGGATGGTTGGTTTGGTCGGCAGAGCGGCCTCTCAATTTATTATTCTATGCCGAATTTAACAATAGAGGTCCAGGAGCTGATTTAACTAGTAGAGTCAAATGGCCAGGTTTTCATGCGATAAATAGTCCATCAGAAGTCATTAATTATACCGTGAATATTTTCATTGATGGAAATGCATGGTTACCAGAAACTAGCGTACCATTTAGACCTACCTTATAG

mRNA sequence

ATGAACTTTGTTGATAGCTTTACTTTTAACACTGTTGTCTCTTTAGATGGAACTGGAAATTTTAGAAGCATTAATGCAGCTATAGCAGCTGCACCAAGTAATAGAAAATCTCGATTTTACATTCATGTGAAACCTGGGATTTACAAAGAACATGTTGAAATCGAAAAGAAAAAGACTTTCATTGCGTTAATCGGTGACAACGCTGCAAACACCATCATTGTTGATAGTAGAAGCCATGCTACTGGTTTTAACACCTTCCAATCCGCTACATTCGTTGTCAAGGGTGATAACTTTATGGCACAGTCCATAACGTTTGAAAATTCTGCTGGACCTGAAAATGGTCAAGCTGTTGCCCTTCTCAACAACCGCGCCAATCATACGGTATACTACAAATGTGTATTTCTTGGCTATCAAGACACTCTTTATGTCAATGGAGACAATGTATTCTTCAAAGATAGTGAAATTTACGGCACGGTAGATTTCATATTTGGATATGGACAAGCTGTGTATCAAGATTGTAATATATATGCAAGATTAGGCAATAGAACGATTACAATCACAGCACAATCTAAACAATTATGGAACCAAAGAAGTGGCTTCGTTTTCCAAAATTGCAGTGTAATGGCCTCTCCTGAACTCAGGTCGATAGAAAATCTAAATTTTGAAGTTTATCTTGGTCGGCCTTGGAGAGACTATTCGACTGTAATTTTTATGGACTCATTTTTGGACTATCTAGTTAATCCAGCAGGATGGTTGGTTTGGTCGGCAGAGCGGCCTCTCAATTTATTATTCTATGCCGAATTTAACAATAGAGGTCCAGGAGCTGATTTAACTAGTAGAGTCAAATGGCCAGGTTTTCATGCGATAAATAGTCCATCAGAAGTCATTAATTATACCGTGAATATTTTCATTGATGGAAATGCATGGTTACCAGAAACTAGCGTACCATTTAGACCTACCTTATAG

Coding sequence (CDS)

ATGAACTTTGTTGATAGCTTTACTTTTAACACTGTTGTCTCTTTAGATGGAACTGGAAATTTTAGAAGCATTAATGCAGCTATAGCAGCTGCACCAAGTAATAGAAAATCTCGATTTTACATTCATGTGAAACCTGGGATTTACAAAGAACATGTTGAAATCGAAAAGAAAAAGACTTTCATTGCGTTAATCGGTGACAACGCTGCAAACACCATCATTGTTGATAGTAGAAGCCATGCTACTGGTTTTAACACCTTCCAATCCGCTACATTCGTTGTCAAGGGTGATAACTTTATGGCACAGTCCATAACGTTTGAAAATTCTGCTGGACCTGAAAATGGTCAAGCTGTTGCCCTTCTCAACAACCGCGCCAATCATACGGTATACTACAAATGTGTATTTCTTGGCTATCAAGACACTCTTTATGTCAATGGAGACAATGTATTCTTCAAAGATAGTGAAATTTACGGCACGGTAGATTTCATATTTGGATATGGACAAGCTGTGTATCAAGATTGTAATATATATGCAAGATTAGGCAATAGAACGATTACAATCACAGCACAATCTAAACAATTATGGAACCAAAGAAGTGGCTTCGTTTTCCAAAATTGCAGTGTAATGGCCTCTCCTGAACTCAGGTCGATAGAAAATCTAAATTTTGAAGTTTATCTTGGTCGGCCTTGGAGAGACTATTCGACTGTAATTTTTATGGACTCATTTTTGGACTATCTAGTTAATCCAGCAGGATGGTTGGTTTGGTCGGCAGAGCGGCCTCTCAATTTATTATTCTATGCCGAATTTAACAATAGAGGTCCAGGAGCTGATTTAACTAGTAGAGTCAAATGGCCAGGTTTTCATGCGATAAATAGTCCATCAGAAGTCATTAATTATACCGTGAATATTTTCATTGATGGAAATGCATGGTTACCAGAAACTAGCGTACCATTTAGACCTACCTTATAG

Protein sequence

MNFVDSFTFNTVVSLDGTGNFRSINAAIAAAPSNRKSRFYIHVKPGIYKEHVEIEKKKTFIALIGDNAANTIIVDSRSHATGFNTFQSATFVVKGDNFMAQSITFENSAGPENGQAVALLNNRANHTVYYKCVFLGYQDTLYVNGDNVFFKDSEIYGTVDFIFGYGQAVYQDCNIYARLGNRTITITAQSKQLWNQRSGFVFQNCSVMASPELRSIENLNFEVYLGRPWRDYSTVIFMDSFLDYLVNPAGWLVWSAERPLNLLFYAEFNNRGPGADLTSRVKWPGFHAINSPSEVINYTVNIFIDGNAWLPETSVPFRPTL

Homology

[Close]

BLAST of Bhi08M001211 vs. Swiss-Prot
Match: sp|O04887|PME2_CITSI (Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 308.1 bits (788), Expect = 1.1e-82
Identity = 149/309 (48.22%), Postives = 211/309 (68.28%), Query Frame = 0


Query: 10  NTVVSLDGTGNFRSINAAIAAAPSNRKSRFYIHVKPGIYKEHVEIEKKKTFIALIGDNAA 69
           N VV+ DG+GN ++I  A+AAA     SR+ I++K G Y E++E++ K   I  +GD   
Sbjct: 202 NIVVAQDGSGNVKTIQEAVAAASRAGGSRYVIYIKAGTYNENIEVKLKN--IMFVGDGIG 261

Query: 70  NTIIVDSRSHATGFNTFQSATFVVKGDNFMAQSITFENSAGPENGQAVALLNNRANHTVY 129
            TII  S+S   G  TF+SAT  V GDNF+A+ IT  N+AGP N QAVA L + ++ +V+
Sbjct: 262 KTIITGSKSVGGGATTFKSATVAVVGDNFIARDITIRNTAGPNNHQAVA-LRSGSDLSVF 321

Query: 130 YKCVFLGYQDTLYVNGDNVFFKDSEIYGTVDFIFGYGQAVYQDCNIYARL-GNRTITITA 189
           Y+C F GYQDTLYV+    F+++ +IYGTVDFIFG    V Q+CNI+AR   NRT T+TA
Sbjct: 322 YRCSFEGYQDTLYVHSQRQFYRECDIYGTVDFIFGNAAVVLQNCNIFARKPPNRTNTLTA 381

Query: 190 QSKQLWNQRSGFVFQNCSVMASPELRSIENLNFEVYLGRPWRDYSTVIFMDSFLDYLVNP 249
           Q +   NQ +G +  NC V A+ +L+ +++ + + +LGRPW+ YS  +++ +FLD L+NP
Sbjct: 382 QGRTDPNQSTGIIIHNCRVTAASDLKPVQS-SVKTFLGRPWKQYSRTVYIKTFLDSLINP 441

Query: 250 AGWLVWSAERPLNLLFYAEFNNRGPGADLTSRVKWPGFHAINSPSEVINYTVNIFIDGNA 309
           AGW+ WS +  LN L+YAE+ N GPG+   +RVKW G+H + SPS+V  +TV  FI GN+
Sbjct: 442 AGWMEWSGDFALNTLYYAEYMNTGPGSSTANRVKWRGYHVLTSPSQVSQFTVGNFIAGNS 501

Query: 310 WLPETSVPF 318
           WLP T+VPF
Sbjct: 502 WLPATNVPF 506

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BLAST of Bhi08M001211 vs. Swiss-Prot
Match: sp|O81415|PME39_ARATH (Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana OX=3702 GN=PME39 PE=2 SV=1)

HSP 1 Score: 298.5 bits (763), Expect = 8.8e-80
Identity = 144/316 (45.57%), Postives = 206/316 (65.19%), Query Frame = 0


Query: 4   VDSFTFNTVVSLDGTGNFRSINAAIAAAPSNRKSRFYIHVKPGIYKEHVEIEKKKTFIAL 63
           V    +N  V++DGTGNF +IN A+ AAP+  ++RF I++K G Y E+VE+ KKKT I  
Sbjct: 215 VQETNYNLSVAIDGTGNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIMF 274

Query: 64  IGDNAANTIIVDSRSHATGFNTFQSATFVVKGDNFMAQSITFENSAGPENGQAVALLNNR 123
           IGD    T+I  +RS   G++TFQ+ T  VKG  ++A+ I+F NSAGP   QAVA   + 
Sbjct: 275 IGDGIGKTVIKANRSRIDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVA-FRSG 334

Query: 124 ANHTVYYKCVFLGYQDTLYVNGDNVFFKDSEIYGTVDFIFGYGQAVYQDCNIYARLGN-- 183
           ++H+ +Y+C F GYQDTLYV+    F+++ +IYGT+DFIFG    V+Q+ ++YAR  N  
Sbjct: 335 SDHSAFYRCEFDGYQDTLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNPG 394

Query: 184 RTITITAQSKQLWNQRSGFVFQNCSVMASPELRSIENLNFEVYLGRPWRDYSTVIFMDSF 243
             I  TAQS+   +Q +G    NC ++A+P+L  ++  NF+ YLGRPWR YS  + + SF
Sbjct: 395 HKIAFTAQSRNQSDQPTGISILNCRILAAPDLIPVKE-NFKAYLGRPWRKYSRTVIIKSF 454

Query: 244 LDYLVNPAGWLVWSAERPLNLLFYAEFNNRGPGADLTSRVKWPGFHAINSPSEVINYTVN 303
           +D L++PAGWL    +  L  L+Y E+ N GPGA++  RV WPGF  I + +E   +TV 
Sbjct: 455 IDDLIHPAGWLEGKKDFALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVG 514

Query: 304 IFIDGNAWLPETSVPF 318
            FIDG+ WL  T +PF
Sbjct: 515 PFIDGSTWLNSTGIPF 528

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BLAST of Bhi08M001211 vs. Swiss-Prot
Match: sp|Q43062|PME_PRUPE (Pectinesterase/pectinesterase inhibitor PPE8B OS=Prunus persica OX=3760 PE=2 SV=1)

HSP 1 Score: 293.1 bits (749), Expect = 3.7e-78
Identity = 146/315 (46.35%), Postives = 203/315 (64.44%), Query Frame = 0


Query: 5   DSFTFNTVVSLDGTGNFRSINAAIAAAPSNRKSRFYIHVKPGIYKEHVEIEKKKTFIALI 64
           D  + + +V+ DGTGNF ++  A+ AAP     R+ I++K G YKE+VEI+KKK  + +I
Sbjct: 203 DGVSVDAIVAQDGTGNFTNVTDAVLAAPDYSMRRYVIYIKRGTYKENVEIKKKKWNLMMI 262

Query: 65  GDNAANTIIVDSRSHATGFNTFQSATFVVKGDNFMAQSITFENSAGPENGQAVALLNNRA 124
           GD    TII  +RS   G+ TF+SATF V G  F+A+ ITFEN+AGPE  QAVAL ++ +
Sbjct: 263 GDGMDATIISGNRSFVDGWTTFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRSD-S 322

Query: 125 NHTVYYKCVFLGYQDTLYVNGDNVFFKDSEIYGTVDFIFGYGQAVYQDCNIYARLG--NR 184
           + +V+Y+C   GYQDTLY +    F++D +I GTVDFIFG    V+Q+C I A+ G  N+
Sbjct: 323 DLSVFYRCNIRGYQDTLYTHTMRQFYRDCKISGTVDFIFGDATVVFQNCQILAKKGLPNQ 382

Query: 185 TITITAQSKQLWNQRSGFVFQNCSVMASPELRSIENLNFEVYLGRPWRDYSTVIFMDSFL 244
             +ITAQ ++  N+ +G   Q C++ A  +L +    +   YLGRPW+ YS  + M SFL
Sbjct: 383 KNSITAQGRKDPNEPTGISIQFCNITADSDLEAASVNSTPTYLGRPWKLYSRTVIMQSFL 442

Query: 245 DYLVNPAGWLVWSAERPLNLLFYAEFNNRGPGADLTSRVKWPGFHAINSPSEVINYTVNI 304
             ++ P GWL W+ +  LN LFY E+ N GPGA L SRVKWPG+   N  ++  NYTV  
Sbjct: 443 SNVIRPEGWLEWNGDFALNSLFYGEYMNYGPGAGLGSRVKWPGYQVFNESTQAKNYTVAQ 502

Query: 305 FIDGNAWLPETSVPF 318
           FI+GN WLP T V +
Sbjct: 503 FIEGNLWLPSTGVKY 516

[Close]

BLAST of Bhi08M001211 vs. Swiss-Prot
Match: sp|O81301|PME40_ARATH (Probable pectinesterase/pectinesterase inhibitor 40 OS=Arabidopsis thaliana OX=3702 GN=PME40 PE=2 SV=1)

HSP 1 Score: 290.8 bits (743), Expect = 1.8e-77
Identity = 141/316 (44.62%), Postives = 209/316 (66.14%), Query Frame = 0


Query: 4   VDSFTFNTVVSLDGTGNFRSINAAIAAAPSNRKSRFYIHVKPGIYKEHVEIEKKKTFIAL 63
           VD    N VV+ +GTGN+ +I  AI+AAP++ ++RF I++K G Y E++EI ++KT I  
Sbjct: 201 VDETKVNLVVAQNGTGNYTTIGEAISAAPNSSETRFVIYIKCGEYFENIEIPREKTMIMF 260

Query: 64  IGDNAANTIIVDSRSHATGFNTFQSATFVVKGDNFMAQSITFENSAGPENGQAVALLNNR 123
           IGD    T+I  +RS+A G+  F SAT  V+G  F+A+ ++F N AGPE  QAVA L + 
Sbjct: 261 IGDGIGRTVIKANRSYADGWTAFHSATVGVRGSGFIAKDLSFVNYAGPEKHQAVA-LRSS 320

Query: 124 ANHTVYYKCVFLGYQDTLYVNGDNVFFKDSEIYGTVDFIFGYGQAVYQDCNIYARL--GN 183
           ++ + YY+C F  YQDT+YV+    F+++ +IYGTVDFIFG    V+Q+C++YAR    N
Sbjct: 321 SDLSAYYRCSFESYQDTIYVHSHKQFYRECDIYGTVDFIFGDASVVFQNCSLYARRPNPN 380

Query: 184 RTITITAQSKQLWNQRSGFVFQNCSVMASPELRSIENLNFEVYLGRPWRDYSTVIFMDSF 243
           + I  TAQ ++   + +G    +  ++A+P+L  ++  NF+ YLGRPW+ YS  + M SF
Sbjct: 381 QKIIYTAQGRENSREPTGISIISSRILAAPDLIPVQ-ANFKAYLGRPWQLYSRTVIMKSF 440

Query: 244 LDYLVNPAGWLVWSAERPLNLLFYAEFNNRGPGADLTSRVKWPGFHAINSPSEVINYTVN 303
           +D LV+PAGWL W  +  L  L+Y E+ N GPG+++T+RV+WPGF  I +  E   ++V 
Sbjct: 441 IDDLVDPAGWLKWKDDFALETLYYGEYMNEGPGSNMTNRVQWPGFKRIETVEEASQFSVG 500

Query: 304 IFIDGNAWLPETSVPF 318
            FIDGN WL  T +PF
Sbjct: 501 PFIDGNKWLNSTRIPF 514

[Close]

BLAST of Bhi08M001211 vs. Swiss-Prot
Match: sp|Q9M9W7|PME22_ARATH (Putative pectinesterase/pectinesterase inhibitor 22 OS=Arabidopsis thaliana OX=3702 GN=PME22 PE=3 SV=1)

HSP 1 Score: 290.4 bits (742), Expect = 2.4e-77
Identity = 144/310 (46.45%), Postives = 202/310 (65.16%), Query Frame = 0


Query: 10  NTVVSLDGTGNFRSINAAIAAAPSNRKSRFYIHVKPGIYKEHVEIEKKKTFIALIGDNAA 69
           NTVV++DG G +R+IN AI  AP++   R+ I+VK G+YKE+++++KKKT I L+GD   
Sbjct: 240 NTVVAIDGKGKYRTINEAINEAPNHSTKRYVIYVKKGVYKENIDLKKKKTNIMLVGDGIG 299

Query: 70  NTIIVDSRSHATGFNTFQSATFVVKGDNFMAQSITFENSAGPENGQAVALLNNRANHTVY 129
            TII   R+   G  TF++AT  V G  F+A+ ITF N+AGP+N QAVAL  + ++ + +
Sbjct: 300 QTIITGDRNFMQGLTTFRTATVAVSGRGFIAKDITFRNTAGPQNRQAVALRVD-SDQSAF 359

Query: 130 YKCVFLGYQDTLYVNGDNVFFKDSEIYGTVDFIFGYGQAVYQDCNIYAR--LGNRTITIT 189
           Y+C   GYQDTLY +    F++D EIYGT+DFIFG G AV Q+C IY R  L  + +TIT
Sbjct: 360 YRCSVEGYQDTLYAHSLRQFYRDCEIYGTIDFIFGNGAAVLQNCKIYTRVPLPLQKVTIT 419

Query: 190 AQSKQLWNQRSGFVFQNCSVMASPELRSIENLNFEVYLGRPWRDYSTVIFMDSFLDYLVN 249
           AQ ++  NQ +GFV QN  V+A+             YLGRPW+ YS  ++M++++  LV 
Sbjct: 420 AQGRKSPNQNTGFVIQNSYVLATQ----------PTYLGRPWKLYSRTVYMNTYMSQLVQ 479

Query: 250 PAGWLVWSAERPLNLLFYAEFNNRGPGADLTSRVKWPGFHAINSPSEVINYTVNIFIDGN 309
           P GWL W     L+ L+Y E+NN GPG   + RVKWPG+H ++  +  +++TV  FIDG 
Sbjct: 480 PRGWLEWFGNFALDTLWYGEYNNIGPGWRSSGRVKWPGYHIMDKRT-ALSFTVGSFIDGR 537

Query: 310 AWLPETSVPF 318
            WLP T V F
Sbjct: 540 RWLPATGVTF 537

[Close]

BLAST of Bhi08M001211 vs. TAIR10
Match: AT4G02300.1 (Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 298.5 bits (763), Expect = 4.9e-81
Identity = 144/316 (45.57%), Postives = 206/316 (65.19%), Query Frame = 0


Query: 4   VDSFTFNTVVSLDGTGNFRSINAAIAAAPSNRKSRFYIHVKPGIYKEHVEIEKKKTFIAL 63
           V    +N  V++DGTGNF +IN A+ AAP+  ++RF I++K G Y E+VE+ KKKT I  
Sbjct: 215 VQETNYNLSVAIDGTGNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIMF 274

Query: 64  IGDNAANTIIVDSRSHATGFNTFQSATFVVKGDNFMAQSITFENSAGPENGQAVALLNNR 123
           IGD    T+I  +RS   G++TFQ+ T  VKG  ++A+ I+F NSAGP   QAVA   + 
Sbjct: 275 IGDGIGKTVIKANRSRIDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVA-FRSG 334

Query: 124 ANHTVYYKCVFLGYQDTLYVNGDNVFFKDSEIYGTVDFIFGYGQAVYQDCNIYARLGN-- 183
           ++H+ +Y+C F GYQDTLYV+    F+++ +IYGT+DFIFG    V+Q+ ++YAR  N  
Sbjct: 335 SDHSAFYRCEFDGYQDTLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNPG 394

Query: 184 RTITITAQSKQLWNQRSGFVFQNCSVMASPELRSIENLNFEVYLGRPWRDYSTVIFMDSF 243
             I  TAQS+   +Q +G    NC ++A+P+L  ++  NF+ YLGRPWR YS  + + SF
Sbjct: 395 HKIAFTAQSRNQSDQPTGISILNCRILAAPDLIPVKE-NFKAYLGRPWRKYSRTVIIKSF 454

Query: 244 LDYLVNPAGWLVWSAERPLNLLFYAEFNNRGPGADLTSRVKWPGFHAINSPSEVINYTVN 303
           +D L++PAGWL    +  L  L+Y E+ N GPGA++  RV WPGF  I + +E   +TV 
Sbjct: 455 IDDLIHPAGWLEGKKDFALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVG 514

Query: 304 IFIDGNAWLPETSVPF 318
            FIDG+ WL  T +PF
Sbjct: 515 PFIDGSTWLNSTGIPF 528

[Close]

BLAST of Bhi08M001211 vs. TAIR10
Match: AT4G02320.1 (Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 290.8 bits (743), Expect = 1.0e-78
Identity = 141/316 (44.62%), Postives = 209/316 (66.14%), Query Frame = 0


Query: 4   VDSFTFNTVVSLDGTGNFRSINAAIAAAPSNRKSRFYIHVKPGIYKEHVEIEKKKTFIAL 63
           VD    N VV+ +GTGN+ +I  AI+AAP++ ++RF I++K G Y E++EI ++KT I  
Sbjct: 201 VDETKVNLVVAQNGTGNYTTIGEAISAAPNSSETRFVIYIKCGEYFENIEIPREKTMIMF 260

Query: 64  IGDNAANTIIVDSRSHATGFNTFQSATFVVKGDNFMAQSITFENSAGPENGQAVALLNNR 123
           IGD    T+I  +RS+A G+  F SAT  V+G  F+A+ ++F N AGPE  QAVA L + 
Sbjct: 261 IGDGIGRTVIKANRSYADGWTAFHSATVGVRGSGFIAKDLSFVNYAGPEKHQAVA-LRSS 320

Query: 124 ANHTVYYKCVFLGYQDTLYVNGDNVFFKDSEIYGTVDFIFGYGQAVYQDCNIYARL--GN 183
           ++ + YY+C F  YQDT+YV+    F+++ +IYGTVDFIFG    V+Q+C++YAR    N
Sbjct: 321 SDLSAYYRCSFESYQDTIYVHSHKQFYRECDIYGTVDFIFGDASVVFQNCSLYARRPNPN 380

Query: 184 RTITITAQSKQLWNQRSGFVFQNCSVMASPELRSIENLNFEVYLGRPWRDYSTVIFMDSF 243
           + I  TAQ ++   + +G    +  ++A+P+L  ++  NF+ YLGRPW+ YS  + M SF
Sbjct: 381 QKIIYTAQGRENSREPTGISIISSRILAAPDLIPVQ-ANFKAYLGRPWQLYSRTVIMKSF 440

Query: 244 LDYLVNPAGWLVWSAERPLNLLFYAEFNNRGPGADLTSRVKWPGFHAINSPSEVINYTVN 303
           +D LV+PAGWL W  +  L  L+Y E+ N GPG+++T+RV+WPGF  I +  E   ++V 
Sbjct: 441 IDDLVDPAGWLKWKDDFALETLYYGEYMNEGPGSNMTNRVQWPGFKRIETVEEASQFSVG 500

Query: 304 IFIDGNAWLPETSVPF 318
            FIDGN WL  T +PF
Sbjct: 501 PFIDGNKWLNSTRIPF 514

[Close]

BLAST of Bhi08M001211 vs. TAIR10
Match: AT3G05620.1 (Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 290.4 bits (742), Expect = 1.3e-78
Identity = 144/310 (46.45%), Postives = 202/310 (65.16%), Query Frame = 0


Query: 10  NTVVSLDGTGNFRSINAAIAAAPSNRKSRFYIHVKPGIYKEHVEIEKKKTFIALIGDNAA 69
           NTVV++DG G +R+IN AI  AP++   R+ I+VK G+YKE+++++KKKT I L+GD   
Sbjct: 240 NTVVAIDGKGKYRTINEAINEAPNHSTKRYVIYVKKGVYKENIDLKKKKTNIMLVGDGIG 299

Query: 70  NTIIVDSRSHATGFNTFQSATFVVKGDNFMAQSITFENSAGPENGQAVALLNNRANHTVY 129
            TII   R+   G  TF++AT  V G  F+A+ ITF N+AGP+N QAVAL  + ++ + +
Sbjct: 300 QTIITGDRNFMQGLTTFRTATVAVSGRGFIAKDITFRNTAGPQNRQAVALRVD-SDQSAF 359

Query: 130 YKCVFLGYQDTLYVNGDNVFFKDSEIYGTVDFIFGYGQAVYQDCNIYAR--LGNRTITIT 189
           Y+C   GYQDTLY +    F++D EIYGT+DFIFG G AV Q+C IY R  L  + +TIT
Sbjct: 360 YRCSVEGYQDTLYAHSLRQFYRDCEIYGTIDFIFGNGAAVLQNCKIYTRVPLPLQKVTIT 419

Query: 190 AQSKQLWNQRSGFVFQNCSVMASPELRSIENLNFEVYLGRPWRDYSTVIFMDSFLDYLVN 249
           AQ ++  NQ +GFV QN  V+A+             YLGRPW+ YS  ++M++++  LV 
Sbjct: 420 AQGRKSPNQNTGFVIQNSYVLATQ----------PTYLGRPWKLYSRTVYMNTYMSQLVQ 479

Query: 250 PAGWLVWSAERPLNLLFYAEFNNRGPGADLTSRVKWPGFHAINSPSEVINYTVNIFIDGN 309
           P GWL W     L+ L+Y E+NN GPG   + RVKWPG+H ++  +  +++TV  FIDG 
Sbjct: 480 PRGWLEWFGNFALDTLWYGEYNNIGPGWRSSGRVKWPGYHIMDKRT-ALSFTVGSFIDGR 537

Query: 310 AWLPETSVPF 318
            WLP T V F
Sbjct: 540 RWLPATGVTF 537

[Close]

BLAST of Bhi08M001211 vs. TAIR10
Match: AT4G33220.1 (pectin methylesterase 44)

HSP 1 Score: 290.4 bits (742), Expect = 1.3e-78
Identity = 148/312 (47.44%), Postives = 204/312 (65.38%), Query Frame = 0


Query: 8   TFNTVVSLDGTGNFRSINAAIAAAPSNRKSRFYIHVKPGIYKEHVEIEKKKTFIALIGDN 67
           T++  V+LDGTGNF  I  AI  AP    +RF I++K G+Y E+VEI+KKK  I ++GD 
Sbjct: 210 TYDVSVALDGTGNFTKIMDAIKKAPDYSSTRFVIYIKKGLYLENVEIKKKKWNIVMLGDG 269

Query: 68  AANTIIVDSRSHATGFNTFQSATFVVKGDNFMAQSITFENSAGPENGQAVALLNNRANHT 127
              T+I  +RS   G+ TF+SATF V G  F+A+ ITF+N+AGPE  QAVAL ++ ++ +
Sbjct: 270 IDVTVISGNRSFIDGWTTFRSATFAVSGRGFLARDITFQNTAGPEKHQAVALRSD-SDLS 329

Query: 128 VYYKCVFLGYQDTLYVNGDNVFFKDSEIYGTVDFIFGYGQAVYQDCNIYARLG--NRTIT 187
           V+++C   GYQDTLY +    F+++  I GTVDFIFG G  V+Q+C I A+ G  N+  T
Sbjct: 330 VFFRCAMRGYQDTLYTHTMRQFYRECTITGTVDFIFGDGTVVFQNCQILAKRGLPNQKNT 389

Query: 188 ITAQSKQLWNQRSGFVFQNCSVMASPELRSIENLNFEVYLGRPWRDYSTVIFMDSFLDYL 247
           ITAQ ++  NQ SGF  Q  ++ A  +L    N     YLGRPW+ YS  +F+ + +  +
Sbjct: 390 ITAQGRKDVNQPSGFSIQFSNISADADLVPYLNTT-RTYLGRPWKLYSRTVFIRNNMSDV 449

Query: 248 VNPAGWLVWSAERPLNLLFYAEFNNRGPGADLTSRVKWPGFHAINSPSEVINYTVNIFID 307
           V P GWL W+A+  L+ LFY EF N GPG+ L+SRVKWPG+H  N+  +  N+TV+ FI 
Sbjct: 450 VRPEGWLEWNADFALDTLFYGEFMNYGPGSGLSSRVKWPGYHVFNNSDQANNFTVSQFIK 509

Query: 308 GNAWLPETSVPF 318
           GN WLP T V F
Sbjct: 510 GNLWLPSTGVTF 519

[Close]

BLAST of Bhi08M001211 vs. TAIR10
Match: AT3G43270.1 (Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 289.3 bits (739), Expect = 3.0e-78
Identity = 145/311 (46.62%), Postives = 202/311 (64.95%), Query Frame = 0


Query: 10  NTVVSLDGTGNFRSINAAIAAAPSNRKSRFYIHVKPGIYKEHVEIEKKKTFIALIGDNAA 69
           + VV+ DGTGNF +I+ A+ AAP     R+ IHVK G+Y E+VEI+KKK  I ++GD   
Sbjct: 214 DAVVAADGTGNFTTISDAVLAAPDYSTKRYVIHVKRGVYVENVEIKKKKWNIMMVGDGID 273

Query: 70  NTIIVDSRSHATGFNTFQSATFVVKGDNFMAQSITFENSAGPENGQAVALLNNRANHTVY 129
            T+I  +RS   G+ TF+SATF V G  F+A+ ITF+N+AGPE  QAVA+ ++  +  V+
Sbjct: 274 ATVITGNRSFIDGWTTFRSATFAVSGRGFIARDITFQNTAGPEKHQAVAIRSD-TDLGVF 333

Query: 130 YKCVFLGYQDTLYVNGDNVFFKDSEIYGTVDFIFGYGQAVYQDCNIYARLG--NRTITIT 189
           Y+C   GYQDTLY +    FF++  I GTVDFIFG   AV+Q C I A+ G  N+  +IT
Sbjct: 334 YRCAMRGYQDTLYAHSMRQFFRECIITGTVDFIFGDATAVFQSCQIKAKQGLPNQKNSIT 393

Query: 190 AQSKQLWNQRSGFVFQNCSVMASPELRSIENLN-FEVYLGRPWRDYSTVIFMDSFLDYLV 249
           AQ ++  N+ +GF  Q  ++ A  +L  + NLN    YLGRPW+ YS  +FM +++   +
Sbjct: 394 AQGRKDPNEPTGFTIQFSNIAADTDL--LLNLNTTATYLGRPWKLYSRTVFMQNYMSDAI 453

Query: 250 NPAGWLVWSAERPLNLLFYAEFNNRGPGADLTSRVKWPGFHAINSPSEVINYTVNIFIDG 309
           NP GWL W+    L+ L+Y E+ N GPGA L  RVKWPG+H +N+ +E  N+TV+  I G
Sbjct: 454 NPVGWLEWNGNFALDTLYYGEYMNSGPGASLDRRVKWPGYHVLNTSAEANNFTVSQLIQG 513

Query: 310 NAWLPETSVPF 318
           N WLP T + F
Sbjct: 514 NLWLPSTGITF 521

[Close]

BLAST of Bhi08M001211 vs. TrEMBL
Match: tr|A0A0A0LR14|A0A0A0LR14_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G049450 PE=4 SV=1)

HSP 1 Score: 377.9 bits (969), Expect = 2.3e-101
Identity = 182/318 (57.23%), Postives = 236/318 (74.21%), Query Frame = 0


Query: 1   MNFVDSFTFNTVVSLDGTGNFRSINAAIAAAPSNRKSRFYIHVKPGIYKEHVEIEKKKTF 60
           M  + S +FN  VSLDG+GNF+ I+ AIAAAP+N  +RFYIHV PG Y E ++I + K F
Sbjct: 18  MLLIHSLSFNLTVSLDGSGNFKRISDAIAAAPNNSNTRFYIHVAPGTYHETLQIPRLKKF 77

Query: 61  IALIGDNAANTIIVDSRSHATGFNTFQSATFVVKGDNFMAQSITFENSAGPENGQAVALL 120
           IAL+GD+   TII+D+RS+ TGF T  SAT  V+GDNFMA+S+TFENSAGP+N QAVA+ 
Sbjct: 78  IALVGDDPFTTIIIDNRSNGTGFKTNNSATLTVQGDNFMAESLTFENSAGPQNNQAVAVF 137

Query: 121 NNRANHTVYYKCVFLGYQDTLYVNGDNVFFKDSEIYGTVDFIFGYGQAVYQDCNIYARLG 180
            ++ANHT YYKC FL +QDTLYVNG   FFK+S+IYG+VDFI GYGQ ++QDCNIYAR+ 
Sbjct: 138 -DKANHTAYYKCRFLSFQDTLYVNGKPQFFKESDIYGSVDFICGYGQVMFQDCNIYARMP 197

Query: 181 NRTITITAQSKQLWNQRSGFVFQNCSVMASPELRSIENLNFEVYLGRPWRDYSTVIFMDS 240
             +IT+TAQSK +    SGF FQNC+V  S E+ S    N +V+LGRPW+ YS V+FM+S
Sbjct: 198 INSITVTAQSKYILRSVSGFSFQNCTVTVSREISS-NKQNVKVFLGRPWKQYSKVVFMES 257

Query: 241 FLDYLVNPAGWLVWSAERPLNLLFYAEFNNRGPGADLTSRVKWPGFHAINSPSEVINYTV 300
           FLD +V   GW+ W    P+N LFY EFNN GPGAD++ RV W  +H ++  S  + +TV
Sbjct: 258 FLDDVVASEGWVEWIGV-PVNNLFYGEFNNCGPGADVSKRVNWTSYHLLDKES-ALRFTV 317

Query: 301 NIFIDGNAWLPETSVPFR 319
           + F++G+ WLPET +PFR
Sbjct: 318 DNFVNGSEWLPETGIPFR 331

[Close]

BLAST of Bhi08M001211 vs. TrEMBL
Match: tr|A0A0A0L1M3|A0A0A0L1M3_CUCSA (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_4G260430 PE=4 SV=1)

HSP 1 Score: 362.8 bits (930), Expect = 7.7e-97
Identity = 180/318 (56.60%), Postives = 221/318 (69.50%), Query Frame = 0


Query: 1   MNFVDSFTFNTVVSLDGTGNFRSINAAIAAAPSNRKSRFYIHVKPGIYKEHVEIEKKKTF 60
           + F D F+FN+ VSLDGT NF  IN AIAAAP+   +RFYIHVKPG Y+E +E+  +KT 
Sbjct: 13  VEFSDCFSFNSTVSLDGTENFIKINDAIAAAPNFSTTRFYIHVKPGTYQEIIEVPYEKTC 72

Query: 61  IALIGDNAANTIIVDSRSHATGFNTFQSATFVVKGDNFMAQSITFENSAGPENGQAVALL 120
           IALIGD+                    SAT  V G NFMAQ +TF+N+AGP  GQA+ALL
Sbjct: 73  IALIGDDXXXXXXXXXXXXXXXXXXASSATLTVNGSNFMAQFLTFQNTAGPNEGQAIALL 132

Query: 121 NNRANHTVYYKCVFLGYQDTLYVNGDNVFFKDSEIYGTVDFIFGYGQAVYQDCNIYARLG 180
            ++A HT YYKCVFLGYQDTLY      FFK+ +IYG+VDFIFG G  V+QDCNIYARL 
Sbjct: 133 -DQATHTAYYKCVFLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLF 192

Query: 181 NRTITITAQSKQLWNQRSGFVFQNCSVMASPELRSIENLNFEVYLGRPWRDYSTVIFMDS 240
           +  IT+TAQSK   N  SGF+FQNC V  SPE+ S ++    V+LGRPWR YS V+F+DS
Sbjct: 193 DVQITVTAQSKPSLNSLSGFIFQNCKVTVSPEIASSKD-KVTVFLGRPWRMYSMVVFIDS 252

Query: 241 FLDYLVNPAGWLVWSAERPLNLLFYAEFNNRGPGADLTSRVKWPGFHAINSPSEVINYTV 300
           FLD +V P GWL W    P NLL+YAE+NN G GA+ + RV WPG+HA+N+  EV  +TV
Sbjct: 253 FLDNVVQPKGWLEWPGV-PENLLYYAEYNNSGGGANTSQRVNWPGYHALNNAKEVATFTV 312

Query: 301 NIFIDGNAWLPETSVPFR 319
            IFI+G  WLP+T +PFR
Sbjct: 313 EIFINGTQWLPQTGIPFR 327

[Close]

BLAST of Bhi08M001211 vs. TrEMBL
Match: tr|A0A1S3CQ52|A0A1S3CQ52_CUCME (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103503419 PE=4 SV=1)

HSP 1 Score: 351.7 bits (901), Expect = 1.8e-93
Identity = 170/306 (55.56%), Postives = 218/306 (71.24%), Query Frame = 0


Query: 13  VSLDGTGNFRSINAAIAAAPSNRKSRFYIHVKPGIYKEHVEIEKKKTFIALIGDNAANTI 72
           +SLDGTGNF  +N AIAAAP+   +RFYIHVKPG Y+E +E+  +KTFIALIGD+     
Sbjct: 19  LSLDGTGNFVKVNDAIAAAPNFSMTRFYIHVKPGTYQEIIEVPSEKTFIALIGDDXXXXX 78

Query: 73  IVDSRSHATGFNTFQSATFVVKGDNFMAQSITFENSAGPENGQAVALLNNRANHTVYYKC 132
                       T  SAT  V G+NFMAQ +TF+NSAGP  GQA+A+L ++A HT YY+C
Sbjct: 79  XXXXXXXXXXXXTASSATLTVYGENFMAQFLTFQNSAGPNEGQAIAVL-DQAKHTAYYRC 138

Query: 133 VFLGYQDTLYVNGDNVFFKDSEIYGTVDFIFGYGQAVYQDCNIYARLGNRTITITAQSKQ 192
           VFLGYQDTLY      FFK+ +IYG+VDFIFG G  V+QDCNIYARL +  IT+TAQSKQ
Sbjct: 139 VFLGYQDTLYAGSVPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLFDVQITVTAQSKQ 198

Query: 193 LWNQRSGFVFQNCSVMASPELRSIENLNFEVYLGRPWRDYSTVIFMDSFLDYLVNPAGWL 252
             +  SGF+FQNC+V  SPE+ S ++    V+LGRPWR YS V+F+DSFLD +V P GWL
Sbjct: 199 SLSAPSGFIFQNCNVTVSPEIASSKD-KVTVFLGRPWRMYSMVVFIDSFLDSVVQPKGWL 258

Query: 253 VWSAERPLNLLFYAEFNNRGPGADLTSRVKWPGFHAINSPSEVINYTVNIFIDGNAWLPE 312
            W    P N+L+YAE+NN G GA+ + RV WPG+H +++  EV  ++V +FI+G  WLP+
Sbjct: 259 EWPGV-PENVLYYAEYNNSGGGANTSQRVNWPGYHVLDNAREVATFSVEMFINGTQWLPQ 318

Query: 313 TSVPFR 319
           T +PFR
Sbjct: 319 TGIPFR 321

[Close]

BLAST of Bhi08M001211 vs. TrEMBL
Match: tr|A0A1S3CP85|A0A1S3CP85_CUCME (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103503231 PE=4 SV=1)

HSP 1 Score: 327.4 bits (838), Expect = 3.6e-86
Identity = 158/274 (57.66%), Postives = 202/274 (73.72%), Query Frame = 0


Query: 45  PGIYKEHVEIEKKKTFIALIGDNAANTIIVDSRSHATGFNTFQSATFVVKGDNFMAQSIT 104
           PG Y E ++I K K FIALIGD+A  TII+D+RS+ TGF T  SAT  V+G+NFMA+S+T
Sbjct: 1   PGTYHEAIQIPKAKKFIALIGDDAFTTIIIDNRSNGTGFKTNNSATLTVQGNNFMAKSLT 60

Query: 105 FENSAGPENGQAVALLNNRANHTVYYKCVFLGYQDTLYVNGDNVFFKDSEIYGTVDFIFG 164
           FENSAGP+NGQAVA+  ++A+HT YYKC FLG+QDTLYVNG   FFK+S IYG+VDFIFG
Sbjct: 61  FENSAGPQNGQAVAVF-DKADHTAYYKCRFLGFQDTLYVNGKYQFFKESNIYGSVDFIFG 120

Query: 165 YGQAVYQDCNIYARLGNRTITITAQSKQLWNQRSGFVFQNCSVMASPELRSIENLNFEVY 224
           +G+ ++QDCNIYAR+ + +IT+TAQSK      SGF FQN +V   PE+ S    N +V+
Sbjct: 121 HGRVMFQDCNIYARMPSNSITVTAQSKDFLRSISGFSFQNYTVTVLPEISS-NKQNVKVF 180

Query: 225 LGRPWRDYSTVIFMDSFLDYLVNPAGWLVWSAERPLNLLFYAEFNNRGPGADLTSRVKWP 284
           LGR WR YS V+FM+SFLD +V   GW+ W    P+N LFY EFNN GPGAD++ RV W 
Sbjct: 181 LGRLWRQYSNVVFMESFLDDVVASEGWMEWKGV-PINNLFYGEFNNYGPGADVSKRVNWT 240

Query: 285 GFHAINSPSEVINYTVNIFIDGNAWLPETSVPFR 319
           G+H +N  S    +TV+ FI+ + WL ET +PFR
Sbjct: 241 GYHILNKES-AFRFTVDNFINRSKWLHETGIPFR 270

[Close]

BLAST of Bhi08M001211 vs. TrEMBL
Match: tr|A0A2R6QI07|A0A2R6QI07_ACTCH (Pectinesterase OS=Actinidia chinensis var. chinensis OX=1590841 GN=CEY00_Acc07184 PE=4 SV=1)

HSP 1 Score: 323.2 bits (827), Expect = 6.8e-85
Identity = 158/308 (51.30%), Postives = 216/308 (70.13%), Query Frame = 0


Query: 13  VSLDGTGNFRSINAAIAAAPSNRKSRFYIHVKPGIYKEHVEIEKKKTFIALIGDNAANTI 72
           +  D +GN+ SIN AIAAAP N KSR+YIHVKPG+YKE+V++  KKT IALIGD+AA TI
Sbjct: 18  IGSDRSGNYTSINDAIAAAPENSKSRYYIHVKPGVYKEYVQV--KKTNIALIGDDAATTI 77

Query: 73  IVDSRSHATGFNTFQSATFVVKGDNFMAQSITFENSAGPENGQAVALLNNRANHTVYYKC 132
           I  ++S+A GF T+ +AT  V G  F+A S+T ENSA   N      LN+ A+ + +YKC
Sbjct: 78  ITGNKSNAEGFKTYDTATMFVGGPGFIAVSLTIENSAPQPNSHQAVALNSIADRSAFYKC 137

Query: 133 VFLGYQDTLYVNGDNVFFKDSEIYGTVDFIFGYGQAVYQDCNIYARLGNRTITITAQSKQ 192
           VFLGYQDTLY + +  F+K  +IYG+VDFIFG  +A++QDCNIYAR       ITAQ+K+
Sbjct: 138 VFLGYQDTLYADHNRQFYKKCDIYGSVDFIFGSAKAMFQDCNIYARRFPLENVITAQAKE 197

Query: 193 LWN--QRSGFVFQNCSVMASPELRSIENLNFEVYLGRPWRDYSTVIFMDSFLDYLVNPAG 252
                + SGF FQNC +  +PE    ++   + YLGRPWR+Y+TV+FM+S+LD +V P G
Sbjct: 198 SDPNIKDSGFSFQNCKITVAPEAVPHKS-EVKAYLGRPWRNYATVVFMESYLDDIVAPEG 257

Query: 253 WLVWSAERPLNLLFYAEFNNRGPGADLTSRVKWPGFHAINSPSEVINYTVNIFIDGNAWL 312
           W+ +        +FYAE+NNRGPGA+ + RVKWPG+    S +E I +TV+ +IDG+ W+
Sbjct: 258 WMPFGNN---TKMFYAEYNNRGPGANTSKRVKWPGYKVFTSATEAIPFTVSKWIDGDTWI 317

Query: 313 PETSVPFR 319
           PET +P++
Sbjct: 318 PETGIPYK 319

[Close]

BLAST of Bhi08M001211 vs. NCBI nr
Match: XP_022150325.1 (probable pectinesterase/pectinesterase inhibitor 44 [Momordica charantia])

HSP 1 Score: 401.4 bits (1030), Expect = 3.0e-108
Identity = 197/321 (61.37%), Postives = 241/321 (75.08%), Query Frame = 0


Query: 1   MNFVDSFTFNTVVSLDGTGNFRSINAAIAAAPSNRKSRFYIHVKPGIYKEHVEIEKKKTF 60
           + F  SFTFN  VSLDG GNF  +N AIAAAP+   SRFYIHVKPG Y EHVE+  +KTF
Sbjct: 21  VEFSYSFTFNITVSLDGMGNFFRVNDAIAAAPNFSTSRFYIHVKPGTYNEHVEVPAQKTF 80

Query: 61  IALIGDNAANTIIVDSRSHATGFNTFQSATFVVKGDNFMAQSITFENSAGPENGQAVALL 120
           IALIGD+A+ TIIVD+RS+ TGF+T  SAT  V G+NFMAQS+TF+NSAGP+ GQAVA+L
Sbjct: 81  IALIGDDASTTIIVDNRSNGTGFSTATSATLTVSGENFMAQSLTFQNSAGPDKGQAVAVL 140

Query: 121 NNRANHTVYYKCVFLGYQDTLYVNGDNVFFKDSEIYGTVDFIFGYGQAVYQDCNIYARLG 180
            ++A HTVYYKCVFLGYQDTL+      FFK+ +IYG+VDFIFG G A++QDCNIYARL 
Sbjct: 141 -DQAKHTVYYKCVFLGYQDTLFAGAVPQFFKECDIYGSVDFIFGNGLAIFQDCNIYARLS 200

Query: 181 NRTITITAQSKQLWNQRSGFVFQNCSVMASPELRSIENLNFEVYLGRPWRDYSTVIFMDS 240
           +  IT+TAQSK   +  SGF+FQ C+V  SPE+ S +N    V+LGRPWR YS V F++S
Sbjct: 201 DIHITVTAQSKDNVDNHSGFIFQACNVTVSPEIVSSKN-KVTVFLGRPWRTYSMVAFIES 260

Query: 241 FLDYLVNPAGWLVWSAERPLNLLFYAEFNNRGPGADLTSRVKWPGFHAINSPSEVINYTV 300
           FLD +V P GWL W    P+NLLFYAE+ N G G++ ++RV WPGFH  +  +EV N+TV
Sbjct: 261 FLDDVVQPKGWLEWPG-TPVNLLFYAEYKNSGTGSETSNRVDWPGFHLFSYAAEVANFTV 320

Query: 301 NIFIDGNAWLPETSVPFRPTL 322
             FIDG  WLPET +PFR  L
Sbjct: 321 ENFIDGTQWLPETGIPFRTGL 338

[Close]

BLAST of Bhi08M001211 vs. NCBI nr
Match: XP_022137308.1 (probable pectinesterase/pectinesterase inhibitor 39 [Momordica charantia])

HSP 1 Score: 399.8 bits (1026), Expect = 8.6e-108
Identity = 198/313 (63.26%), Postives = 231/313 (73.80%), Query Frame = 0


Query: 9   FNTVVSLDGTGNFRSINAAIAAAPSNRKSRFYIHVKPGIYKEHVEIEKKKTFIALIGDNA 68
           FN  VSLDG+G++++I  AIAAAP N  +RFYIHV  G Y EHVEI   KTF+ALIGD+A
Sbjct: 28  FNATVSLDGSGDYKTIKDAIAAAPDNSITRFYIHVMAGTYNEHVEIPSTKTFVALIGDDA 87

Query: 69  ANTIIVDSRSHATGFNTFQSATFVVKGDNFMAQSITFENSAGPENGQAVALLNNRANHTV 128
           + TIIVD+RS+  GF TF SATF VKG NFMA+SITF+NSAGPENGQAVALLN+ A+ TV
Sbjct: 88  STTIIVDNRSNRIGFKTFHSATFNVKGRNFMAKSITFKNSAGPENGQAVALLND-AHQTV 147

Query: 129 YYKCVFLGYQDTLYVNGDNVFFKDSEIYGTVDFIFGYGQAVYQDCNIYARLGNRTITITA 188
           YY C+FLG+QDTLYV GD                        +DC IY  L N T+TITA
Sbjct: 148 YYNCIFLGFQDTLYVRGD------------------------KDCKIYVGLSNTTVTITA 207

Query: 189 QSKQLWNQRSGFVFQNCSVMASPELRSIENLNFEVYLGRPWRDYSTVIFMDSFLDYLVNP 248
           QSK+L ++ SGF FQNCSV  SPEL   ++ NF+VYLGRP RDYSTVIFM SFLD +VNP
Sbjct: 208 QSKELESEHSGFSFQNCSVTTSPELGPRKDQNFDVYLGRPSRDYSTVIFMQSFLDTVVNP 267

Query: 249 AGWLVWSAERPLNLLFYAEFNNRGPGADLTSRVKWPGFHAINSPSEVINYTVNIFIDGNA 308
            GWL WS   PLNLLFYAEFNNRGPGAD++ R+KW G+H IN+P E   YTV  FIDG  
Sbjct: 268 KGWLEWSLGSPLNLLFYAEFNNRGPGADISKRIKWQGYHLINNPLEATKYTVKDFIDGTT 315

Query: 309 WLPETSVPFRPTL 322
           WLPET+VPFRP+L
Sbjct: 328 WLPETNVPFRPSL 315

[Close]

BLAST of Bhi08M001211 vs. NCBI nr
Match: XP_023538248.1 (probable pectinesterase/pectinesterase inhibitor 39 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 396.4 bits (1017), Expect = 9.5e-107
Identity = 194/321 (60.44%), Postives = 243/321 (75.70%), Query Frame = 0


Query: 1   MNFVDSFTFNTVVSLDGTGNFRSINAAIAAAPSNRKSRFYIHVKPGIYKEHVEIEKKKTF 60
           + F ++F FN+ VSLDGTGNF  I+ AI AAP+   +RFYIH+KPGIY EH+E+  +KTF
Sbjct: 19  LKFSNTFDFNSTVSLDGTGNFVKISDAIVAAPNFTTTRFYIHIKPGIYNEHIEVPFQKTF 78

Query: 61  IALIGDNAANTIIVDSRSHATGFNTFQSATFVVKGDNFMAQSITFENSAGPENGQAVALL 120
           I+LIGDNA+ TIIVD+RS ATGF+T  SAT  V G NFMA+ +TF+NSAGP+ GQAVA+L
Sbjct: 79  ISLIGDNASTTIIVDNRSKATGFSTANSATLTVNGANFMAKFLTFQNSAGPKMGQAVAVL 138

Query: 121 NNRANHTVYYKCVFLGYQDTLYVNGDNVFFKDSEIYGTVDFIFGYGQAVYQDCNIYARLG 180
            ++A HT YYKCVFLGYQDTLY      FFK+ +IYGTVDFIFG G  V+QDCNIYARL 
Sbjct: 139 -DQAKHTAYYKCVFLGYQDTLYAAAVPQFFKECDIYGTVDFIFGNGLVVFQDCNIYARLV 198

Query: 181 NRTITITAQSKQLWNQRSGFVFQNCSVMASPELRSIENLNFEVYLGRPWRDYSTVIFMDS 240
           +   T+TAQSK   +++SGFVFQNC+V  SPE+ S  +    V+LGRPWR YS V F++S
Sbjct: 199 DIHTTVTAQSKANLDEQSGFVFQNCTVTVSPEIASSRD-KVLVFLGRPWRAYSMVAFVES 258

Query: 241 FLDYLVNPAGWLVWSAERPLNLLFYAEFNNRGPGADLTSRVKWPGFHAINSPSEVINYTV 300
           FLD +V P GWL W    P+NLL+Y E+NNRG GA+ + RV WPGFH ++S +EV N+TV
Sbjct: 259 FLDNVVQPKGWLEWLGV-PVNLLYYGEYNNRGGGANTSQRVDWPGFHVLHSLTEVANFTV 318

Query: 301 NIFIDGNAWLPETSVPFRPTL 322
             FI+G  WLPET++PFR  L
Sbjct: 319 ENFINGTTWLPETTIPFRAGL 336

[Close]

BLAST of Bhi08M001211 vs. NCBI nr
Match: XP_022150332.1 (probable pectinesterase/pectinesterase inhibitor 39 [Momordica charantia])

HSP 1 Score: 395.6 bits (1015), Expect = 1.6e-106
Identity = 190/321 (59.19%), Postives = 240/321 (74.77%), Query Frame = 0


Query: 1   MNFVDSFTFNTVVSLDGTGNFRSINAAIAAAPSNRKSRFYIHVKPGIYKEHVEIEKKKTF 60
           + F   F FN  VS DG+GNF  ++ AIAAAP+   +RFYIHVKPGIYKEHVE+  +KTF
Sbjct: 21  VKFSYCFMFNATVSPDGSGNFVRVSDAIAAAPNFSTTRFYIHVKPGIYKEHVEVPVQKTF 80

Query: 61  IALIGDNAANTIIVDSRSHATGFNTFQSATFVVKGDNFMAQSITFENSAGPENGQAVALL 120
           IALIG++A+ TIIVD+RS+ +GF+T  +AT +VKG NFMAQ +TFENSA  + GQA+A++
Sbjct: 81  IALIGEDASTTIIVDNRSNGSGFSTQTTATLIVKGQNFMAQFLTFENSAEQKEGQAIAVM 140

Query: 121 NNRANHTVYYKCVFLGYQDTLYVNGDNVFFKDSEIYGTVDFIFGYGQAVYQDCNIYARLG 180
            ++A HT YYKCVFL YQDTLY      FFK+ +IYG +DFIFG G  V+QDCNIYAR+ 
Sbjct: 141 -DQAKHTAYYKCVFLSYQDTLYAGAVPQFFKECDIYGRIDFIFGNGLVVFQDCNIYARIP 200

Query: 181 NRTITITAQSKQLWNQRSGFVFQNCSVMASPELRSIENLNFEVYLGRPWRDYSTVIFMDS 240
              I +TAQSK     +SGF+FQNC+V  SPE+ S ++ N  ++LGRPWRDYS VIFM+S
Sbjct: 201 GTHIIVTAQSKNAVEDQSGFIFQNCNVTVSPEIASSKD-NVTMFLGRPWRDYSMVIFMES 260

Query: 241 FLDYLVNPAGWLVWSAERPLNLLFYAEFNNRGPGADLTSRVKWPGFHAINSPSEVINYTV 300
           FLD +V P GWL W   RP+NLLFYAE+NNRGPGAD + RV WPG+H +++  EV  +TV
Sbjct: 261 FLDRVVQPKGWLEWPG-RPVNLLFYAEYNNRGPGADTSQRVDWPGYHVLHNAMEVTKFTV 320

Query: 301 NIFIDGNAWLPETSVPFRPTL 322
             FI+G  WLPET +PFR  L
Sbjct: 321 ENFINGTKWLPETGIPFRAGL 338

[Close]

BLAST of Bhi08M001211 vs. NCBI nr
Match: XP_023511664.1 (probable pectinesterase/pectinesterase inhibitor 7 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 394.8 bits (1013), Expect = 2.8e-106
Identity = 197/318 (61.95%), Postives = 236/318 (74.21%), Query Frame = 0


Query: 4   VDSFTFNTVVSLDGTGNFRSINAAIAAAPSNRKSRFYIHVKPGIYKEHVEIEKKKTFIAL 63
           VD   FN  VSLDG+G+F+SI+ AIAAAP+N  +RFYI V PGIY+ H+EI   K FIAL
Sbjct: 21  VDPIRFNATVSLDGSGDFKSISEAIAAAPNNSNTRFYIRVTPGIYRGHLEIPPAKKFIAL 80

Query: 64  IGDNAANTIIVDSRSHATGFNTFQSATFVVKGDNFMAQSITFENSAGPENGQAVALLNNR 123
           IGDNA  TIIVD+RSH TGF T +S T VV GDNFMAQ ++FENSA P+NGQAVA+L + 
Sbjct: 81  IGDNALTTIIVDNRSHGTGFKTSESTTLVVNGDNFMAQHLSFENSAEPQNGQAVAVL-DY 140

Query: 124 ANHTVYYKCVFLGYQDTLYVNGDNVFFKDSEIYGTVDFIFGYGQAVYQDCNIYARLGNRT 183
           A HTVYY C FL +QDTLY  G+  FF++ +IYGTVDFIFG G AV+QDCNIYARL    
Sbjct: 141 AKHTVYYDCKFLSFQDTLYSKGEFQFFRECDIYGTVDFIFGNGLAVFQDCNIYARLPMDE 200

Query: 184 ITITAQSKQLWNQRSGFVFQNCSVMASPELRSIENLNFEVYLGRPWRDYSTVIFMDSFLD 243
           IT+TAQSK+    +SGF FQNC+V  SPE+ +    N ++YLGRPWR+YSTV+FM+SFLD
Sbjct: 201 ITVTAQSKEQSMSQSGFSFQNCTVTVSPEI-TANKTNVKIYLGRPWREYSTVVFMESFLD 260

Query: 244 YLVNPAGWLVWSAERPLNLLFYAEFNNRGPGADLTSRVKWPGFHAINSPSEVINYTVNIF 303
             V P GW+ W    P+N LFYAEFNNRGPGAD T RV WP FH ++      N+TV  F
Sbjct: 261 DNVQPQGWISWGGV-PINNLFYAEFNNRGPGADTTHRVNWPTFHVLDK-LLAKNFTVENF 320

Query: 304 IDGNAWLPETSVPFRPTL 322
           I+G+ WLPET VPFR  L
Sbjct: 321 INGSDWLPETHVPFRTGL 334

The following BLAST results are available for this feature:

BLAST of Bhi08M001211 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|O04887\|PME2_CITSI	1.1e-82	48.22	Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1	[more]
sp\|O81415\|PME39_ARATH	8.8e-80	45.57	Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana OX=3...	[more]
sp\|Q43062\|PME_PRUPE	3.7e-78	46.35	Pectinesterase/pectinesterase inhibitor PPE8B OS=Prunus persica OX=3760 PE=2 SV=...	[more]
sp\|O81301\|PME40_ARATH	1.8e-77	44.62	Probable pectinesterase/pectinesterase inhibitor 40 OS=Arabidopsis thaliana OX=3...	[more]
sp\|Q9M9W7\|PME22_ARATH	2.4e-77	46.45	Putative pectinesterase/pectinesterase inhibitor 22 OS=Arabidopsis thaliana OX=3...	[more]

BLAST of Bhi08M001211 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G02300.1	4.9e-81	45.57	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT4G02320.1	1.0e-78	44.62	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT3G05620.1	1.3e-78	46.45	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT4G33220.1	1.3e-78	47.44	pectin methylesterase 44	[more]
AT3G43270.1	3.0e-78	46.62	Plant invertase/pectin methylesterase inhibitor superfamily	[more]

BLAST of Bhi08M001211 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0LR14\|A0A0A0LR14_CUCSA	2.3e-101	57.23	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G049450 PE=4 SV=1	[more]
tr\|A0A0A0L1M3\|A0A0A0L1M3_CUCSA	7.7e-97	56.60	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_4G260430 PE=4 SV=1	[more]
tr\|A0A1S3CQ52\|A0A1S3CQ52_CUCME	1.8e-93	55.56	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103503419 PE=4 SV=1	[more]
tr\|A0A1S3CP85\|A0A1S3CP85_CUCME	3.6e-86	57.66	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103503231 PE=4 SV=1	[more]
tr\|A0A2R6QI07\|A0A2R6QI07_ACTCH	6.8e-85	51.30	Pectinesterase OS=Actinidia chinensis var. chinensis OX=1590841 GN=CEY00_Acc0718...	[more]

BLAST of Bhi08M001211 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022150325.1	3.0e-108	61.37	probable pectinesterase/pectinesterase inhibitor 44 [Momordica charantia]	[more]
XP_022137308.1	8.6e-108	63.26	probable pectinesterase/pectinesterase inhibitor 39 [Momordica charantia]	[more]
XP_023538248.1	9.5e-107	60.44	probable pectinesterase/pectinesterase inhibitor 39 [Cucurbita pepo subsp. pepo]	[more]
XP_022150332.1	1.6e-106	59.19	probable pectinesterase/pectinesterase inhibitor 39 [Momordica charantia]	[more]
XP_023511664.1	2.8e-106	61.95	probable pectinesterase/pectinesterase inhibitor 7 [Cucurbita pepo subsp. pepo]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **Biological Process**
Term	Definition
GO:0042545	cell wall modification

Vocabulary: **Cellular Component**
Term	Definition
GO:0005618	cell wall

Vocabulary: **Molecular Function**
Term	Definition
GO:0030599	pectinesterase activity

Vocabulary: **INTERPRO**
Term	Definition
IPR011050	Pectin_lyase_fold/virulence
IPR033131	Pectinesterase_Asp_AS
IPR012334	Pectin_lyas_fold
IPR000070	Pectinesterase_cat

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
cellular_component	GO:0005618	cell wall
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0046910	pectinesterase inhibitor activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Bhi08G001211	Bhi08G001211	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Bhi08M001211.exon2	Bhi08M001211.exon2	exon
Bhi08M001211.exon1	Bhi08M001211.exon1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.Bhi08M001211	cds.Bhi08M001211	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Bhi08M001211	Bhi08M001211-protein	polypeptide

InterPro

Analysis Name: InterPro Annotations of wax gourd
Date Performed: 2019-11-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 10..307 e-value: 1.3E-108 score: 362.1
IPR012334	Pectin lyase fold	GENE3D	G3DSA:2.160.20.10		coord: 5..321 e-value: 1.4E-117 score: 394.5
None	No IPR available	PANTHER	PTHR31707	FAMILY NOT NAMED	coord: 4..321
None	No IPR available	PANTHER	PTHR31707:SF25	PECTINESTERASE/PECTINESTERASE INHIBITOR 39-RELATED	coord: 4..321
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 155..164
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	SSF51126	Pectin lyase-like	coord: 10..319