Bhi04M000183 (mRNA) Wax gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Bhi04M000183
Type	mRNA
Organism	Benincasa hispida (Wax gourd)
Description	ribosomal RNA small subunit methyltransferase I
Location	chr4 : 5004894 .. 5010945 (+)
Sequence length	1718

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTAATTATATAAAATCTTGTCCCATTACAAATGTCTAGTAAAATTTCAAAGGGCGGCTTTACTCCAATTTGGCGGATTTTTGGAGAATTGTTCCTTTAATCTTCCTTCTACCGTCGTCCCCATTTACAATTCAATTCAATTCGCTTGCTCCGGCATTAATGTTGACGCGACGAATTCCTTCAGTGGCAGTTTATCTCCCGCAAGCTCTGCCATGGCGTCGTGGACCTCCTCACTCTATCAACCCCAGGACGCCGCTTGAATTCCACTCCTTCCTCGCCTTTTGCCCCAAAAGTTGTTTCGTTTCTTTTTGTTCTGCTTCTCAATCCATCTCTGAAGCTGTAGAACCAATTCATGCGGAAGAATCCTTGAAGCGCGTAAGACCATGAATTACTTCCCAGAGCTTAAGTTCTTTTATATGCATTCTTAACCATTTCCTTTGTGGGCTTGAAAATTAGCACAAACCCAGTTAGTGATTAGCAGAGATAATGACATACATGGTTATAGAGAGAACATAAGACTGAACCCCTAAAGCTTAAAGTGAAAAGTATGCATAATCTTTTATACGTTTTCTTAACGGTTATGTTTTTGTTGGCTCTCCATGTTGAAACCGTTGTTCCACTTAGGCTACTTCTCTTTCTGACACCTCTATTGGATTGGATTCTACTTCAACTACTGGATTTTTAATCTATGTCTTTCTTAGTACCCGATCAGAATTTGTCTTTCTTAGTACCCGATCAGAATTTAAGTTATAGCAACTTCGTAAGTCGTAAAACGAAACTAGGAGGTGCATTCTGTCCGTTTTTCATGGTTTAAGATGCGTTGTCTTTAAACTTTGACAGACATTCTTGTCTTTTGATCTACGTGATTGTAGGAACCCTTGAAACCTGGGTTATATCTGGTTGGCACGCCAATTGGCAATCTTGAAGATATCACCCTACGGTACGAAAGTTCGATAAAGAGCACCAACATTATTGTCCTTTCTTTTCAGCATATATGCATAAACACTGAAAGCTTGACACTCAGCATTGACAATTTGCTATTTTCTTTTCATGCGTACATCTCTAAACTCATAATTTGGAAATAGACTTTATGTTTTGTGATATTTCTAGCTGACTCATTTCCTGGAGTAGGATGAAGTTCTTAAGTTTAGGATCTCCTCCTTTATACTGGTCCATATTTGATGACGTTATTCAGTTATTTGGAATAATCTGCCTATTTATCTCCATAAAATTCTATTACACAGTGCTCTACGTGTCTTAAAGTCAGCACACGTCATCCTTTCAGAGGATACAAGACACTCAGGAAAGTTGCTTCAGCATTTCAGCATTAGAACCCCTCTTGTGAGTTTGTTGAGGCATTTATTTTCTGACATTATAGTTTTCTTTTCCTTCTTCATTTGTTCTGATGAAGGCTTTTAGATTTCAGCTCAGTTTTCACAAATTCAACGAGTCTCAAAGAGAGCAAACAGTAGTGAAGAGATTAAAAGAGGGTGAAATTGTTGCCCTAATTAGTGACGCAGGGACACCAGGCATTAGTGACCCTGGTACTGAGTTGGTGAGCATGCTCTAAACAATTTTAAATTTAGTTCTTTCATTAAGGCTCTGTTAAGCATCTATCACTTTTATAATCTACATTTATTGGGCTCATTTTCAATGTGCTGATTGACCATTCACATTTCATAAAAATCTGAGACTTGATAATGTAACCAATTAATTTGAAAGGAAAAACTTGAGAGCGGATACTCAGAGAGGGGAAATGAAAAATCAAAACGCTATGGAAATTCCTATAGAAGTTATCATCTAGAGAATCAAAACTACTTTTGAAGGTTCCGCTGTAGACTATAGTCATAGCCAAGGAAAAATACTAGAACAAAGCCATGCATTTTCATATTGGCGTTCTTCTTAATATTTTATTGGTTTATTATGAAAGGTGTTAATATTTTTTAGTTCAAGAACAAGCAGGGATCGAAGAGAAAAAAAGAAAAAGAAAAAAAAAGGTTCAAGCAGGGATGAGAAGATTTGAACCTCCAACCTCTTAGTTGCAGGCATATGCTTTTAACTAATTGAGCTATGCTAAAGTCGTAATTATGAAAGGTGGTATTAATATAATTGTACCATGTGAATACAGGTTAGATTATGTGTCAATAAAAATATACCCGTTATTCCTGTTCCTGGGCCCTCTGCTGTTGTTGCTGCTATTTCTGCCTCTGGCCTGTCAACCGATGAGTTTACATTTGGTAAATTTCATACTGGTACTTATAATTGTGCTTTGACTGCAAGGGCATCTTTCTTTGAGCTTTTTTTGCCCTATGGCTACCTTCAGGCTTGCTACAGTTTCTCAGTATGTGTATAGTGTGCAGTTGGTTTTGTTCCTAAGCATGCTGCATCTAGAAGAGAGAGGCTGATGGTTTCAGCAAGCGAGGAGGCAACTCAAATATTCTATGTTCCTCCGCACAAACTCAAGCAGTTTCTCGAGGAAACTTCACAATTATTTGGTGAATCAAGGTACTTCGTATAATCCAATCTACTGTACTAATAATTCTCTCGGGGCAATTTATCAGTGCAGTCCCTCTTCCACTATCTGGTTCAATCTATATAATTCATTAACTCATGACATCTGATTTGGAACCTTTGAGGTAATTAGTTGTATGAAGATACTTTAATCATCATATCTTAATTAGTCTATTTCATTAATGTTCAAGTTGGGTTGGGAAAAGGTTATGATGTATTTGGCTTGTTCTAGTGTTTGAAGACAATAAACAAACTCACTAATTCTGCAGTTAAGCTTGCATCATGCATTATCCTGTTATGTTCTATCCAAGTCATCAAGGATTTGTTAGACATTGTATAACTTGGAAGCTTGTGTGTGTTTCTTTGAATAATGACTGTGGGTACTGGTTGTATAATATTGTTCATGTGCTTCTAGTTACATATGTGGTTATTTGGAAATGATTTTGAAATGGTTAAAACAACTTTTGTTATGTCAAAAAACACTCAAAAACATGCTGCTAATATTGAAAATCAATGATTATACCATGTATGAATTCGTGTTTAGAAGTGTAATATCAAACATTAAATTTATTTTGAAATGATGAAAGACATATTCGAGTGATTTTACCATTTCAAAATGACTCCCAAACATCCCTATAATCGAAACTCATATTGTGATCCAAATTGCTTTAGTGGAGGAGCAGACTCATCTTTTGTTAGTAGCCAGTTTGCTTATTGAATATGTTTGTTTCCGTTACTGTTTTGCAGGCAATGTGTCATAGCTCGTGAAATGACCAAGATACACGAGGAGGTAACTTTCTTCTTTACTCTATTTTTTGCTATTTTAGTTGGTAGGGTTCCCAATACTTCTTACACGAAAAACTGATCCAACCAACTAGTGGTCACGATTGGTGTCAGTTTATTGATTTTTTTAAAAAAAACTGGTTGGTAGGTCGATCCAAAGAAAGGGGGGAAAAAAAACCAACCGACCAACTGACCAATATGTAATCCTATAATATATTATTGGTCTTTTTAAGTTTCATTATTTTTTAAAAACAAAATAAATCCCCAGCCCAACCCACTTTCTCCAGCCCAATCCACATCCCTTTCAGACTTTCTCTAAATTGGCCCTCAATCAGACAAAACAGAAACCCTTACCATTCAAAAAATAAATAAATAAAGAGAGAGAGAGAGTGGACAAAACTGACCGCTGGTCGGTTGGCTGCCTTACAAAGGAGCGTGCAAATCGGTAGAAAACCTGTTGGTTTCGGTGCTGGTTTTCCCCCAAAACCAACCCTAACCGACCGATGTATGCCCCTACTTCCAATTATGAGTTTATAAGTTTAAGCATCAAAATAATAGTAAAAGTAAATAAAAAAGTCAACTCCATACGATAACATAAGAAGCATGCTTAGTTCGATTTAGCTAATGTGTCCGTTTTAACTTGGACACTCTAACATTTGTTGAATACAAATTGAACACCTTCTAGCACAATTAATAAATATGTATTAGAAACTAATTGTTCAAAGTTAATATGAGTCCAACATTTCTTAGATACGTATCGAAAAATGAATATTTGTTATTTAAGCATACAAAATGCCAAAAAAAAATAATAAACTTCGAGTGCAAATTTCATCGAACTCATTTTATAAGCATATATATGTCCATAAACTTATTGCTTTTGAATTTCCATATTGTATAAAAATAATATATATTTATAAAAATGCATATTTTAATAAGTGCATCCTTACTATGTCTGTGTCCTAAATATGTAAAAACAAAGTGTCATTGTGTCGTATGTATCTATGTCTCTTATCCATATTGATGCTCCTTCAGTGACAAGGTAAGACCGAAATGACGGCTAAACATATATTTAGATACAGTAACTAGATTTTACGGAACAGGGCCAACACGCTGCTCAACACAAATGTGATTGGTTGCAATTTGCAATTATTATTTGAAAACATTTTACTCATCCTGAAAGCAAGAAAGCTTACGTGGAATTTTCATTTCATCATTCTTGTGATATACAATTTGTCGCTCTCTTTTTCTTGTGGAGGATTTGATTCCTGTACATGGGAACTGGGTTATACTATCATTTAGAATAGAACATATATGCTAAGTGGCCAATGGCCATATATTTTAGTTGATGTTGTCTTTGATCTACTTTCTATCAATTATTTAACTACCATGATCTTGTGTTAATTCTTTTGATATGTTGTGATACGTTAGTGTTATTCATGCATGAATACTGTAATTTCAAGGGTTTCCAATAATAGATTTCACAATATTCACTTGACTTGTCATTTAAAAATAAGCATGATGATTGCAACAGTTTTGGCGGGGTACCCTGGCTGAAGCTAAAGAATTCTTCTCGCTGCACCAGACGAAGGGTGAAATTACGCTGCTGATTGAAGGAAATCTAAATTCTGAAGTCGAAACTCCATCAGAATCACAGCTGGAAAAGGAGTTGGAAGAATTGATTTCCAGCGGACATAGGCTTTCTACGGTAATGACTTGAATTGGACTATAGGATAAAGACAATTTTATGAAAAGTAATTCATTTTCAGAGGCTTGGTATCATTCTCTCAGTACTGGACAAAGAGGAGTGTCTTGGTCTGAACTTTCAGGTTTCTTCTAGTTTAGTCTCCAAAGATTTGAATAATAACTATTTAGCCATGGCTGATATTTGACCATCCAATACATGGATGACATGACTTCAATGTCTCATTGTGTTTCATATCATGTCATAGTGGTGGCATTTATTGAAGTTGTATAAGCTGGTTAAGGGTGGGTAGTGAGGGGAAGATATTAGATAGTAGTGAACAAAATGATTATTTTGTTAAAATTCAAAGTTTAAATAGAAATTTTTAAGTTCAGGAAAGAATATAGAATAGATCTTACAACTTTCGAGATGGGAACAAACCACGAACCATAGAAATATTGTAGTCCCTGAATTCTGACAAAACTTTATTTTCGTGAAGGCTGTCAAATTGGTTGCTAGCAAAACATCAGCACGTAGGAAAACTGTCTATTCAATAGCATTGCGAAGATTCGGGAATCAACTTGGAGCGGAGAACGATTCTTGTAAATAATGATTGTCTGTAGCATGCTTGCCACAGGTGTAAGTTGTTAATTGCTTATAATCATCTCTCCATTCTTGCAAAATGACTACTTTGTATCAACTGTTCTGGTAATCTTCTGAAGAACAACTTGCCTTTCCATATACATTTTGACAGTTGTTGGTTTTGTTCTAATCTTTTCGTTAGATATTATTGGGGTCGGTGGGACAAAGATATATGATTTGTGCTAGAATAGTGGCTATATAAGAAGCATAGATACTGTAACACGTGAACTTTTTTAAGGAAAAAATTAGGTCGTGGACACGTATTTACTAAAATATATATTTTTCAAGACATATATCATCTTTATATAATAAGGAAATTCATAGTTACAAGTTTATGCAAAATAATCTCGATATATTTTGCTCTTAAAATTTATTAGCTTTGTCCCATGTTACTATTTGTGCCTTCTAGTCCGTTTGAGTGTTTGATGCATGCGTT

mRNA sequence

CTAATTATATAAAATCTTGTCCCATTACAAATGTCTAGTAAAATTTCAAAGGGCGGCTTTACTCCAATTTGGCGGATTTTTGGAGAATTGTTCCTTTAATCTTCCTTCTACCGTCGTCCCCATTTACAATTCAATTCAATTCGCTTGCTCCGGCATTAATGTTGACGCGACGAATTCCTTCAGTGGCAGTTTATCTCCCGCAAGCTCTGCCATGGCGTCGTGGACCTCCTCACTCTATCAACCCCAGGACGCCGCTTGAATTCCACTCCTTCCTCGCCTTTTGCCCCAAAAGTTGTTTCGTTTCTTTTTGTTCTGCTTCTCAATCCATCTCTGAAGCTGTAGAACCAATTCATGCGGAAGAATCCTTGAAGCGCGAACCCTTGAAACCTGGGTTATATCTGGTTGGCACGCCAATTGGCAATCTTGAAGATATCACCCTACGTGCTCTACGTGTCTTAAAGTCAGCACACGTCATCCTTTCAGAGGATACAAGACACTCAGGAAAGTTGCTTCAGCATTTCAGCATTAGAACCCCTCTTCTCAGTTTTCACAAATTCAACGAGTCTCAAAGAGAGCAAACAGTAGTGAAGAGATTAAAAGAGGGTGAAATTGTTGCCCTAATTAGTGACGCAGGGACACCAGGCATTAGTGACCCTGGTACTGAGTTGGTTAGATTATGTGTCAATAAAAATATACCCGTTATTCCTGTTCCTGGGCCCTCTGCTGTTGTTGCTGCTATTTCTGCCTCTGGCCTGTCAACCGATGAGTTTACATTTGTTGGTTTTGTTCCTAAGCATGCTGCATCTAGAAGAGAGAGGCTGATGGTTTCAGCAAGCGAGGAGGCAACTCAAATATTCTATGTTCCTCCGCACAAACTCAAGCAGTTTCTCGAGGAAACTTCACAATTATTTGGTGAATCAAGGCAATGTGTCATAGCTCGTGAAATGACCAAGATACACGAGGAGTTTTGGCGGGGTACCCTGGCTGAAGCTAAAGAATTCTTCTCGCTGCACCAGACGAAGGGTGAAATTACGCTGCTGATTGAAGGAAATCTAAATTCTGAAGTCGAAACTCCATCAGAATCACAGCTGGAAAAGGAGTTGGAAGAATTGATTTCCAGCGGACATAGGCTTTCTACGGCTGTCAAATTGGTTGCTAGCAAAACATCAGCACGTAGGAAAACTGTCTATTCAATAGCATTGCGAAGATTCGGGAATCAACTTGGAGCGGAGAACGATTCTTGTAAATAATGATTGTCTGTAGCATGCTTGCCACAGGTGTAAGTTGTTAATTGCTTATAATCATCTCTCCATTCTTGCAAAATGACTACTTTGTATCAACTGTTCTGGTAATCTTCTGAAGAACAACTTGCCTTTCCATATACATTTTGACAGTTGTTGGTTTTGTTCTAATCTTTTCGTTAGATATTATTGGGGTCGGTGGGACAAAGATATATGATTTGTGCTAGAATAGTGGCTATATAAGAAGCATAGATACTGTAACACGTGAACTTTTTTAAGGAAAAAATTAGGTCGTGGACACGTATTTACTAAAATATATATTTTTCAAGACATATATCATCTTTATATAATAAGGAAATTCATAGTTACAAGTTTATGCAAAATAATCTCGATATATTTTGCTCTTAAAATTTATTAGCTTTGTCCCATGTTACTATTTGTGCCTTCTAGTCCGTTTGAGTGTTTGATGCATGCGTT

Coding sequence (CDS)

ATGTTGACGCGACGAATTCCTTCAGTGGCAGTTTATCTCCCGCAAGCTCTGCCATGGCGTCGTGGACCTCCTCACTCTATCAACCCCAGGACGCCGCTTGAATTCCACTCCTTCCTCGCCTTTTGCCCCAAAAGTTGTTTCGTTTCTTTTTGTTCTGCTTCTCAATCCATCTCTGAAGCTGTAGAACCAATTCATGCGGAAGAATCCTTGAAGCGCGAACCCTTGAAACCTGGGTTATATCTGGTTGGCACGCCAATTGGCAATCTTGAAGATATCACCCTACGTGCTCTACGTGTCTTAAAGTCAGCACACGTCATCCTTTCAGAGGATACAAGACACTCAGGAAAGTTGCTTCAGCATTTCAGCATTAGAACCCCTCTTCTCAGTTTTCACAAATTCAACGAGTCTCAAAGAGAGCAAACAGTAGTGAAGAGATTAAAAGAGGGTGAAATTGTTGCCCTAATTAGTGACGCAGGGACACCAGGCATTAGTGACCCTGGTACTGAGTTGGTTAGATTATGTGTCAATAAAAATATACCCGTTATTCCTGTTCCTGGGCCCTCTGCTGTTGTTGCTGCTATTTCTGCCTCTGGCCTGTCAACCGATGAGTTTACATTTGTTGGTTTTGTTCCTAAGCATGCTGCATCTAGAAGAGAGAGGCTGATGGTTTCAGCAAGCGAGGAGGCAACTCAAATATTCTATGTTCCTCCGCACAAACTCAAGCAGTTTCTCGAGGAAACTTCACAATTATTTGGTGAATCAAGGCAATGTGTCATAGCTCGTGAAATGACCAAGATACACGAGGAGTTTTGGCGGGGTACCCTGGCTGAAGCTAAAGAATTCTTCTCGCTGCACCAGACGAAGGGTGAAATTACGCTGCTGATTGAAGGAAATCTAAATTCTGAAGTCGAAACTCCATCAGAATCACAGCTGGAAAAGGAGTTGGAAGAATTGATTTCCAGCGGACATAGGCTTTCTACGGCTGTCAAATTGGTTGCTAGCAAAACATCAGCACGTAGGAAAACTGTCTATTCAATAGCATTGCGAAGATTCGGGAATCAACTTGGAGCGGAGAACGATTCTTGTAAATAA

Protein sequence

MLTRRIPSVAVYLPQALPWRRGPPHSINPRTPLEFHSFLAFCPKSCFVSFCSASQSISEAVEPIHAEESLKREPLKPGLYLVGTPIGNLEDITLRALRVLKSAHVILSEDTRHSGKLLQHFSIRTPLLSFHKFNESQREQTVVKRLKEGEIVALISDAGTPGISDPGTELVRLCVNKNIPVIPVPGPSAVVAAISASGLSTDEFTFVGFVPKHAASRRERLMVSASEEATQIFYVPPHKLKQFLEETSQLFGESRQCVIAREMTKIHEEFWRGTLAEAKEFFSLHQTKGEITLLIEGNLNSEVETPSESQLEKELEELISSGHRLSTAVKLVASKTSARRKTVYSIALRRFGNQLGAENDSCK

Homology

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BLAST of Bhi04M000183 vs. Swiss-Prot
Match: sp|P74038|RSMI_SYNY3 (Ribosomal RNA small subunit methyltransferase I OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=rsmI PE=3 SV=1)

HSP 1 Score: 236.5 bits (602), Expect = 4.7e-61
Identity = 124/270 (45.93%), Postives = 171/270 (63.33%), Query Frame = 0


Query: 79  LYLVGTPIGNLEDITLRALRVLKSAHVILSEDTRHSGKLLQHFSIRTPLLSFHKFNESQR 138
           LYLV TPIGNL D+T RA+  L++  +I +EDTRH+GKLLQHF I TP +S+H  N   R
Sbjct: 4   LYLVATPIGNLGDMTPRAVETLQTVDLIAAEDTRHTGKLLQHFQITTPQISYHDHNRHGR 63

Query: 139 EQTVVKRLKEGEIVALISDAGTPGISDPGTELVRLCVNKNIPVIPVPGPSAVVAAISASG 198
            Q ++ +L+ G+ +AL+SDAGTPGISDPG ELV  C   NI VIP+PG +A++AA+ +SG
Sbjct: 64  TQELLAKLQAGQNIALVSDAGTPGISDPGQELVAACGEANIEVIPIPGATALIAALISSG 123

Query: 199 LSTDEFTFVGFVPKHAASRRERLMVSASEEATQIFYVPPHKLKQFLEETSQLFGESRQCV 258
           L+TD F F GF+      R++ L   A EE T I Y  PH+L   L +     G+ R   
Sbjct: 124 LATDRFVFEGFLSTKNRPRQQLLQSLAQEERTIILYEAPHRLLATLTDLQTFLGQERSLT 183

Query: 259 IAREMTKIHEEFWRGTLAEAKEFFSLHQTKGEITLLIEGNLNSEVETPSESQLEKELEEL 318
           +ARE+TK HE+FWRGTL  A  +F+ +  KGE  L+I G    +  + SE  L  EL  L
Sbjct: 184 VARELTKYHEQFWRGTLQTAIAYFTENTPKGEFCLVIAGATPEDRPSFSEENLRDELRSL 243

Query: 319 ISSGHRLSTAVKLVASKTSARRKTVYSIAL 349
           ++ G   S A + +A +T   R+ +Y ++L
Sbjct: 244 MAKGLTRSQASRQLAEETKLPRRQLYQLSL 273

[Close]

BLAST of Bhi04M000183 vs. Swiss-Prot
Match: sp|Q9KGL2|RSMI_BACHD (Ribosomal RNA small subunit methyltransferase I OS=Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) OX=272558 GN=rsmI PE=3 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 3.8e-55
Identity = 124/285 (43.51%), Postives = 172/285 (60.35%), Query Frame = 0


Query: 67  EESLKREPLKPGLYLVGTPIGNLEDITLRALRVLKSAHVILSEDTRHSGKLLQHFSIRTP 126
           ++S ++   K  LYLV TPIGNLED+T RA+R LK A  I +EDTR + KLL HF I T 
Sbjct: 4   QQSYQQRDDKGTLYLVATPIGNLEDVTFRAIRTLKEADQIAAEDTRQTKKLLNHFDIATK 63

Query: 127 LLSFHKFNESQREQTVVKRLKEGEIVALISDAGTPGISDPGTELVRLCVNKNIPVIPVPG 186
           L+S+H+ N+    + ++  L EG  +AL+SDAG P ISDPG ELV   + + I VIP+PG
Sbjct: 64  LVSYHEHNKETMGKRLIDDLIEGRTIALVSDAGMPAISDPGYELVVSAIKEGIAVIPIPG 123

Query: 187 PSAVVAAISASGLSTDEFTFVGFVPKHAASRRERLMVSASEEATQIFYVPPHKLKQFLEE 246
            +A V A+ ASGL T+ F F+GF+P+    RR+ L  +   +AT IFY  PH+LK  L++
Sbjct: 124 ANAAVTALIASGLPTESFQFIGFLPRQKKQRRQALEETKPTKATLIFYESPHRLKDTLDD 183

Query: 247 TSQLFGESRQCVIAREMTKIHEEFWRGTLAEAKEFFSLHQTKGEITLLIEGNLNSEVETP 306
              + G +R   I RE+TK +EEF RGTL EA  +      KGE  L++EG  N E   P
Sbjct: 184 MLLILG-NRHVSICRELTKTYEEFLRGTLEEAVHWAREATIKGEFCLIVEG--NGEKVEP 243

Query: 307 SESQLE-----KELEELISSGHRLSTAVKLVASKTSARRKTVYSI 347
            E   E     + +E  I+ G R   A+K VA+     ++ +Y+I
Sbjct: 244 EEVWWESLSPVQHVEHYIALGFRSKEAIKQVATDRGVPKRDIYNI 285

[Close]

BLAST of Bhi04M000183 vs. Swiss-Prot
Match: sp|P45298|RSMI_HAEIN (Ribosomal RNA small subunit methyltransferase I OS=Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) OX=71421 GN=rsmI PE=3 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 1.5e-51
Identity = 113/272 (41.54%), Postives = 160/272 (58.82%), Query Frame = 0


Query: 79  LYLVGTPIGNLEDITLRALRVLKSAHVILSEDTRHSGKLLQHFSIRTPLLSFHKFNESQR 138
           LY+V TPIGNL+DIT RAL       +I +EDTRHSG LL H+ I+ P  + H  NE ++
Sbjct: 8   LYIVATPIGNLQDITQRALETFAQVDLIAAEDTRHSGLLLSHYGIKKPFFALHDHNEQEK 67

Query: 139 EQTVVKRLKEGEIVALISDAGTPGISDPGTELVRLCVNKNIPVIPVPGPSAVVAAISASG 198
              +V++LK+G  +ALISDAGTP ISDPG  LVR C    I V+P+PG  A + A+ ASG
Sbjct: 68  AHILVEKLKQGSNIALISDAGTPLISDPGFHLVRQCREAGIRVVPLPGACAAITALCASG 127

Query: 199 LSTDEFTFVGFVPKHAASRRERLMVSASEEATQIFYVPPHKLKQFLEETSQLFGESRQCV 258
           +++D F F GF+P  + +R+++L   A E+ T IFY   H++   LE+   + GE R  V
Sbjct: 128 IASDRFCFEGFLPAKSKARKDKLENIAEEDRTLIFYESTHRILDTLEDMQAVLGEERYIV 187

Query: 259 IAREMTKIHEEFWRGTLAEAKEFF--SLHQTKGEITLLIEGNLNSEVETPSESQLEKELE 318
           +AREMTK  E     T+   +E+     ++TKGE+ L++EG   S+       Q  K L 
Sbjct: 188 LAREMTKTWETITGNTIKNLREWLLEDPNRTKGEMVLIVEGKPKSDNNDEISPQAVKAL- 247

Query: 319 ELISSGHRLSTAVKLVASKTSARRKTVYSIAL 349
           ELI+    L  A  +VA     ++  +Y   L
Sbjct: 248 ELIAEELPLKKAAAIVAELYGYKKNALYQFGL 278

[Close]

BLAST of Bhi04M000183 vs. Swiss-Prot
Match: sp|Q9CN04|RSMI_PASMU (Ribosomal RNA small subunit methyltransferase I OS=Pasteurella multocida (strain Pm70) OX=272843 GN=rsmI PE=3 SV=1)

HSP 1 Score: 203.0 bits (515), Expect = 5.7e-51
Identity = 112/275 (40.73%), Postives = 162/275 (58.91%), Query Frame = 0


Query: 79  LYLVGTPIGNLEDITLRALRVLKSAHVILSEDTRHSGKLLQHFSIRTPLLSFHKFNESQR 138
           LY+V TPIGNL+DIT RAL + +   +I +EDTRHSG LL H+ I+ P  + H  NE Q+
Sbjct: 8   LYIVATPIGNLQDITQRALAIFEQVDLIAAEDTRHSGLLLSHYGIKKPFFALHDHNEQQK 67

Query: 139 EQTVVKRLKEGEIVALISDAGTPGISDPGTELVRLCVNKNIPVIPVPGPSAVVAAISASG 198
              +V++L++G+ +ALISDAGTP ISDPG  LVR C    I V+P+PG  A V A+ ASG
Sbjct: 68  AHLLVEKLQQGQHIALISDAGTPLISDPGFHLVRQCRQAGIKVVPIPGACAAVTALCASG 127

Query: 199 LSTDEFTFVGFVPKHAASRRERLMVSASEEATQIFYVPPHKLKQFLEETSQLFGESRQCV 258
           +++D F F GF+P  + +R ++L   A EE T IFY   H++   L +  +  G  R  V
Sbjct: 128 IASDRFCFEGFLPAKSKARCDKLQNLAEEERTLIFYESTHRILDTLADIEKTLGAERYVV 187

Query: 259 IAREMTKIHEEFWRGTLAEAKEFF--SLHQTKGEITLLIEGNLNSEVETPSESQLEKELE 318
           +ARE+TK  E      +A  +++     ++TKGE+ L+IEG +  E +  +   L  +  
Sbjct: 188 LAREITKTWETIVGDNVANLRQWLGEDPNRTKGEMVLIIEGKVKQETDEINPQAL--KAL 247

Query: 319 ELISSGHRLSTAVKLVASKTSARRKTVYSIALRRF 352
           ELIS    L  A  +VA     ++  +Y   L  F
Sbjct: 248 ELISQSLPLKKAAAIVAEIYGYKKNALYQYGLEHF 280

[Close]

BLAST of Bhi04M000183 vs. Swiss-Prot
Match: sp|Q9HVZ3|RSMI_PSEAE (Ribosomal RNA small subunit methyltransferase I OS=Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) OX=208964 GN=rsmI PE=3 SV=1)

HSP 1 Score: 202.2 bits (513), Expect = 9.7e-51
Identity = 121/276 (43.84%), Postives = 166/276 (60.14%), Query Frame = 0


Query: 79  LYLVGTPIGNLEDITLRALRVLKSAHVILSEDTRHSGKLLQHFSIRTPLLSFHKFNESQR 138
           L++V TPIGNL+DI+ RALRVL+   ++ +EDTRHS +L QHF I TPL + H+ NE + 
Sbjct: 6   LFVVATPIGNLDDISPRALRVLREVALVAAEDTRHSIRLFQHFGIETPLAACHEHNEREE 65

Query: 139 EQTVVKRLKEGEIVALISDAGTPGISDPGTELVRLCVNKNIPVIPVPGPSAVVAAISASG 198
               + RL+ GE VALISDAGTP ISDPG  LVR      I V+PVPG  A++AA+SA+G
Sbjct: 66  GGRFISRLQGGEDVALISDAGTPLISDPGFHLVRQAQALGIRVVPVPGSCALIAALSAAG 125

Query: 199 LSTDEFTFVGFVPKHAASRRERLMVSASEEATQIFYVPPHKLKQFLEETSQLFGESRQCV 258
           L +D F F GF+P  AA RR RL     E  T IFY  PH+L + L +   +FG  R+ V
Sbjct: 126 LPSDRFIFEGFLPAKAAGRRSRLQAVQEEPRTLIFYEAPHRLLESLADMRDVFGGERRAV 185

Query: 259 IAREMTKIHEEFWRGTLAEAKEFFS--LHQTKGEITLLIEGNLNSEVETPSESQLEKELE 318
           +ARE++K  E      LAE  ++ +   +Q +GE  +L+ G    E E    ++  + L+
Sbjct: 186 LARELSKTFETIRSLPLAELHDWVASDSNQQRGECVVLVAGWQAPEGEESIGAEALRVLD 245

Query: 319 ELISSGHRLSTAVKLVASKTSARRKTVYSIALRRFG 353
            L++    L  A  L A  T  R+  +Y  AL+R G
Sbjct: 246 LLLAE-LPLKKAAALAAEITGVRKNLLYQQALQRQG 280

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BLAST of Bhi04M000183 vs. TAIR10
Match: AT1G45110.1 (Tetrapyrrole (Corrin/Porphyrin) Methylases)

HSP 1 Score: 429.1 bits (1102), Expect = 2.7e-120
Identity = 216/313 (69.01%), Postives = 265/313 (84.66%), Query Frame = 0


Query: 48  VSFCSASQSISEAVEPIHAEESLKREPLKPGLYLVGTPIGNLEDITLRALRVLKSAHVIL 107
           +SFCS+S+  +++V    A++  KR PLKPGLYLVGTPIGNLEDITLRA+RVL+SA VIL
Sbjct: 31  ISFCSSSE-FADSV----AKDDSKRGPLKPGLYLVGTPIGNLEDITLRAIRVLRSADVIL 90

Query: 108 SEDTRHSGKLLQHFSIRTPLLSFHKFNESQREQTVVKRLKEGEIVALISDAGTPGISDPG 167
           SEDTRHSGKLLQ+++I+  LLS+HKFNE+QREQ V+ RLK+GEIVALISDAGTPGISDPG
Sbjct: 91  SEDTRHSGKLLQYYNIKAQLLSYHKFNEAQREQAVLTRLKQGEIVALISDAGTPGISDPG 150

Query: 168 TELVRLCVNKNIPVIPVPGPSAVVAAISASGLSTDEFTFVGFVPKHAASRRERLMVSASE 227
           T+L ++C  +NI VIP+PG  AVVAA+SASGL TDEFTFVGF+PKH+ +R+ERL+VS++E
Sbjct: 151 TQLAKMCAKENIDVIPIPGACAVVAALSASGLETDEFTFVGFLPKHSGTRKERLIVSSNE 210

Query: 228 EATQIFYVPPHKLKQFLEETSQLFGESRQCVIAREMTKIHEEFWRGTLAEAKEFFSLHQT 287
             TQIFYVPPHKL QFLEET+  FGESRQCVIARE+TK+HEEFWRG++AEAK+ F + Q 
Sbjct: 211 TRTQIFYVPPHKLSQFLEETTPYFGESRQCVIAREITKLHEEFWRGSIAEAKQEFLIRQP 270

Query: 288 KGEITLLIEGNLNSEVETPSESQLEKELEELISSGHRLSTAVKLVASKTSARRKTVYSIA 347
           KGEITLLIEG   ++ E P+ESQLE+EL  LIS GH LSTAVK VA +TS R+K VYS+A
Sbjct: 271 KGEITLLIEGKEETKAENPTESQLEEELRGLISDGHSLSTAVKTVAERTSMRKKEVYSLA 330

Query: 348 LRRFGNQLGAEND 361
           L++FG Q+  E +
Sbjct: 331 LKKFGKQIRVEEE 338

[Close]

BLAST of Bhi04M000183 vs. TrEMBL
Match: tr|A0A0A0LRJ7|A0A0A0LRJ7_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G362440 PE=3 SV=1)

HSP 1 Score: 634.8 bits (1636), Expect = 1.2e-178
Identity = 330/365 (90.41%), Postives = 341/365 (93.42%), Query Frame = 0


Query: 1   MLTRRIPSVAVYLPQALPWRRGPPH--SINPRTPLEFHSFLAFCPKSCFVSFCSASQSIS 60
           ML RRIPSVAVYLPQALPWRR PPH  SIN  T LEFHS LAFCPK+  VS CSASQ   
Sbjct: 1   MLARRIPSVAVYLPQALPWRRRPPHSYSINYGTALEFHSLLAFCPKTLLVSLCSASQLTY 60

Query: 61  EAVEPIHAEESLKREPLKPGLYLVGTPIGNLEDITLRALRVLKSAHVILSEDTRHSGKLL 120
           EAVEPIHAEES KR  LKPGLYLVGTPIGNLEDITLRALRVLKSAHVILSEDTRHSGKLL
Sbjct: 61  EAVEPIHAEESSKRGSLKPGLYLVGTPIGNLEDITLRALRVLKSAHVILSEDTRHSGKLL 120

Query: 121 QHFSIRTPLLSFHKFNESQREQTVVKRLKEGEIVALISDAGTPGISDPGTELVRLCVNKN 180
           QHFSI+TPLLSFHKFNESQREQTV+KRLK+GEIVALISDAGTPGISDPGTELVRLCVN+N
Sbjct: 121 QHFSIKTPLLSFHKFNESQREQTVLKRLKQGEIVALISDAGTPGISDPGTELVRLCVNEN 180

Query: 181 IPVIPVPGPSAVVAAISASGLSTDEFTFVGFVPKHAASRRERLMVSASEEATQIFYVPPH 240
           IPV+PVPGPSAVVAAISASGLSTDEFTFVGF+PKHAASRRERLMVSA+EEATQIFYVPPH
Sbjct: 181 IPVVPVPGPSAVVAAISASGLSTDEFTFVGFLPKHAASRRERLMVSANEEATQIFYVPPH 240

Query: 241 KLKQFLEETSQLFGESRQCVIAREMTKIHEEFWRGTLAEAKEFFSLHQTKGEITLLIEGN 300
           KLKQFLEETSQLFGESR+CVIAREMTKIHEEFWRGTLAEAKEFFSL+QTKGEITLLIEG 
Sbjct: 241 KLKQFLEETSQLFGESRRCVIAREMTKIHEEFWRGTLAEAKEFFSLNQTKGEITLLIEGK 300

Query: 301 LNSEVETPSESQLEKELEELISSGHRLSTAVKLVASKTSARRKTVYSIALRRFGNQLGAE 360
           L  EVETPSESQLEKELEELIS+GHRLS AVKLVASKTS  RKTVYSIALRRFGNQLG E
Sbjct: 301 LCPEVETPSESQLEKELEELISTGHRLSMAVKLVASKTSTSRKTVYSIALRRFGNQLGVE 360

Query: 361 NDSCK 364
           NDSCK
Sbjct: 361 NDSCK 365

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BLAST of Bhi04M000183 vs. TrEMBL
Match: tr|A0A1S3BBU6|A0A1S3BBU6_CUCME (ribosomal RNA small subunit methyltransferase I OS=Cucumis melo OX=3656 GN=LOC103488001 PE=3 SV=1)

HSP 1 Score: 634.0 bits (1634), Expect = 2.0e-178
Identity = 327/365 (89.59%), Postives = 343/365 (93.97%), Query Frame = 0


Query: 1   MLTRRIPSVAVYLPQALPWRRGPP--HSINPRTPLEFHSFLAFCPKSCFVSFCSASQSIS 60
           ML RR+PSVAVYLPQALPWRR PP  HSIN RT L FHS LAFCPK+  VS CSASQ  +
Sbjct: 1   MLARRMPSVAVYLPQALPWRRRPPHSHSINSRTALVFHSLLAFCPKTILVSLCSASQLAT 60

Query: 61  EAVEPIHAEESLKREPLKPGLYLVGTPIGNLEDITLRALRVLKSAHVILSEDTRHSGKLL 120
           EAVEPIHAEE  KR PLKPGLYLVGTPIGNLEDITLRALRVLKSAHVILSEDTRHSGKLL
Sbjct: 61  EAVEPIHAEEFSKRGPLKPGLYLVGTPIGNLEDITLRALRVLKSAHVILSEDTRHSGKLL 120

Query: 121 QHFSIRTPLLSFHKFNESQREQTVVKRLKEGEIVALISDAGTPGISDPGTELVRLCVNKN 180
           QHFSI+TPLLSFHKFNESQREQTV+KRLK+GEIVALISDAGTPGISDPGTELVRLCVN+N
Sbjct: 121 QHFSIKTPLLSFHKFNESQREQTVLKRLKQGEIVALISDAGTPGISDPGTELVRLCVNEN 180

Query: 181 IPVIPVPGPSAVVAAISASGLSTDEFTFVGFVPKHAASRRERLMVSASEEATQIFYVPPH 240
           IPV+PVPGPSAVVAAISASGLSTDEFTFVGF+PKHAA+RRERLMVSA+EEATQIFYVPPH
Sbjct: 181 IPVVPVPGPSAVVAAISASGLSTDEFTFVGFLPKHAATRRERLMVSANEEATQIFYVPPH 240

Query: 241 KLKQFLEETSQLFGESRQCVIAREMTKIHEEFWRGTLAEAKEFFSLHQTKGEITLLIEGN 300
           KLKQFLEETSQLFGESR+CVIAREMTKIHEEFWRGTLAEAKEFFSL+ TKGEITLLIEG 
Sbjct: 241 KLKQFLEETSQLFGESRRCVIAREMTKIHEEFWRGTLAEAKEFFSLNPTKGEITLLIEGK 300

Query: 301 LNSEVETPSESQLEKELEELISSGHRLSTAVKLVASKTSARRKTVYSIALRRFGNQLGAE 360
           L+ EVETPSESQLEKELEELIS+GHRLS AVKLVASKTSA RKTVYSIALRRFGNQLG E
Sbjct: 301 LSPEVETPSESQLEKELEELISTGHRLSMAVKLVASKTSASRKTVYSIALRRFGNQLGVE 360

Query: 361 NDSCK 364
           N+SCK
Sbjct: 361 NNSCK 365

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BLAST of Bhi04M000183 vs. TrEMBL
Match: tr|A0A2P5DMT6|A0A2P5DMT6_PARAD (rRNA small subunit methyltransferase I OS=Parasponia andersonii OX=3476 GN=PanWU01x14_049910 PE=3 SV=1)

HSP 1 Score: 495.4 bits (1274), Expect = 1.1e-136
Identity = 258/366 (70.49%), Postives = 304/366 (83.06%), Query Frame = 0


Query: 1   MLTRRIPSVAVYLPQALPWRRGPPHSINPRTPLEFHSFLAFCPKSCFVSFCSASQSISEA 60
           MLTR + S AV L  A P     P  I+P+T L    +     ++   +FCS+ Q  +++
Sbjct: 1   MLTRLLSSPAVSLAGAFPRLCTFPVFIHPKTSLPVSPY-----RTGAAAFCSSPQGSADS 60

Query: 61  VEPIHAEESLKREPLKPGLYLVGTPIGNLEDITLRALRVLKSAHVILSEDTRHSGKLLQH 120
             PI  EES KR PL+PGLYLVGTPIGNLED+TLRALRVLKSAHVILSEDTRHSGKLLQH
Sbjct: 61  ANPI-PEESSKRGPLEPGLYLVGTPIGNLEDVTLRALRVLKSAHVILSEDTRHSGKLLQH 120

Query: 121 FSIRTPLLSFHKFNESQREQTVVKRLKEGEIVALISDAGTPGISDPGTELVRLCVNKNIP 180
           ++IRTPLLS+HKFNESQREQTV++RLK+GEIVALISDAGTPGISDPGTEL +LC+++NIP
Sbjct: 121 YNIRTPLLSYHKFNESQREQTVLRRLKQGEIVALISDAGTPGISDPGTELAKLCISENIP 180

Query: 181 VIPVPGPSAVVAAISASGLSTDEFTFVGFVPKHAASRRERLMVSASEEATQIFYVPPHKL 240
           V+P+PGPSA VAA+SASGLSTDEFTFVGF+PKH  SR++RLMVSA+E +TQ+FYVPPHKL
Sbjct: 181 VVPIPGPSAFVAALSASGLSTDEFTFVGFLPKHNGSRKDRLMVSANEASTQVFYVPPHKL 240

Query: 241 KQFLEETSQLFGESRQCVIAREMTKIHEEFWRGTLAEAKEFFSLHQTKGEITLLIEGNLN 300
           +QFLEETS LFG+SRQCVIARE+TK+HE+FWRGTL EAKE FS+HQ KGEITLLIEG  +
Sbjct: 241 RQFLEETSPLFGDSRQCVIAREITKLHEQFWRGTLEEAKEAFSVHQPKGEITLLIEGKTS 300

Query: 301 SEVETPSESQLEKELEELISSGHRLSTAVKLVASKTSARRKTVYSIALRRFGNQLGAEN- 360
             VETPSE +LE EL  LISSG+ LSTAVKL A  TS +RKT+YSIALR+FG QLG+E+ 
Sbjct: 301 PTVETPSECELENELSNLISSGYSLSTAVKLAAVGTSVKRKTIYSIALRKFGKQLGSEDI 360

Query: 361 --DSCK 364
             DSCK
Sbjct: 361 DLDSCK 360

[Close]

BLAST of Bhi04M000183 vs. TrEMBL
Match: tr|A0A2C9VZ05|A0A2C9VZ05_MANES (Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_05G154300 PE=3 SV=1)

HSP 1 Score: 488.8 bits (1257), Expect = 1.1e-134
Identity = 256/361 (70.91%), Postives = 293/361 (81.16%), Query Frame = 0


Query: 1   MLTRRIPSVAVYLPQALPWRRGPPHSINPRTPLEFHSFLAFCPKSCFVSFCSASQSISEA 60
           ML RR+P + + L  AL WR  P      +T L     ++  P+   +SFC +SQ   E 
Sbjct: 1   MLIRRLPVMMISLTTALSWRHPPFTFFRTQTDLISLKSVSASPRIAPLSFCYSSQISPEL 60

Query: 61  VEPIHAEESLKREPLKPGLYLVGTPIGNLEDITLRALRVLKSAHVILSEDTRHSGKLLQH 120
              I +E+S KR PLKPGLYLVGTPIGNLEDITLRALRVLKSAHVILSEDTRHSGKLLQ+
Sbjct: 61  TGSI-SEDSSKRGPLKPGLYLVGTPIGNLEDITLRALRVLKSAHVILSEDTRHSGKLLQY 120

Query: 121 FSIRTPLLSFHKFNESQREQTVVKRLKEGEIVALISDAGTPGISDPGTELVRLCVNKNIP 180
           ++IRTPLLS+HKFNESQRE TV+KRLK+GEIVALISDAG PGISDPG EL +LCV++NIP
Sbjct: 121 YNIRTPLLSYHKFNESQRELTVLKRLKQGEIVALISDAGMPGISDPGAELAKLCVDENIP 180

Query: 181 VIPVPGPSAVVAAISASGLSTDEFTFVGFVPKHAASRRERLMVSASEEATQIFYVPPHKL 240
           VIP+PGPSA+VAA+SASGL+TDEFTFVGF+ KH+ SRRERLM SA E  TQIFYVPPHK 
Sbjct: 181 VIPIPGPSALVAALSASGLATDEFTFVGFLSKHSRSRRERLMASADEARTQIFYVPPHKF 240

Query: 241 KQFLEETSQLFGESRQCVIAREMTKIHEEFWRGTLAEAKEFFSLHQTKGEITLLIEGNLN 300
            QFLEETS LFG+SRQCV+ARE+TKIHEEFWRGTL EAKE FS HQ KGEITLLIEG +N
Sbjct: 241 SQFLEETSSLFGDSRQCVMAREITKIHEEFWRGTLGEAKEIFSDHQPKGEITLLIEGKMN 300

Query: 301 SEVETPSESQLEKELEELISSGHRLSTAVKLVASKTSARRKTVYSIALRRFGNQLGAEND 360
           S VE PSESQLE EL +LIS+GH LS AVK+VA KTS RRKT+YS+ALR+ G QL  E+ 
Sbjct: 301 SLVEPPSESQLENELRDLISTGHSLSMAVKMVAEKTSVRRKTIYSLALRKLGKQLEEEDS 360

Query: 361 S 362
           S
Sbjct: 361 S 360

[Close]

BLAST of Bhi04M000183 vs. TrEMBL
Match: tr|A0A2P5EZL8|A0A2P5EZL8_9ROSA (rRNA small subunit methyltransferase I OS=Trema orientalis OX=63057 GN=TorRG33x02_131900 PE=3 SV=1)

HSP 1 Score: 487.3 bits (1253), Expect = 3.1e-134
Identity = 255/366 (69.67%), Postives = 302/366 (82.51%), Query Frame = 0


Query: 1   MLTRRIPSVAVYLPQALPWRRGPPHSINPRTPLEFHSFLAFCPKSCFVSFCSASQSISEA 60
           MLTR + S AV L  A+P        I+ +T L    +     ++   +FCS+ Q  +++
Sbjct: 1   MLTRLLSSPAVSLAGAVPRLCTFRVFIHSKTSLPVSPY-----RTGVATFCSSPQGSTDS 60

Query: 61  VEPIHAEESLKREPLKPGLYLVGTPIGNLEDITLRALRVLKSAHVILSEDTRHSGKLLQH 120
             PI  EES KR PL+PGLYLVGTPIGNLEDITLRALRVLKSAHVILSEDTRHSGKLL+H
Sbjct: 61  ANPI-PEESSKRGPLEPGLYLVGTPIGNLEDITLRALRVLKSAHVILSEDTRHSGKLLRH 120

Query: 121 FSIRTPLLSFHKFNESQREQTVVKRLKEGEIVALISDAGTPGISDPGTELVRLCVNKNIP 180
           ++IRTPLLS+HKFNESQREQTV++RLK+GEIVALISDAGTPGISDPGTEL +LC+++NIP
Sbjct: 121 YNIRTPLLSYHKFNESQREQTVLRRLKQGEIVALISDAGTPGISDPGTELAKLCISENIP 180

Query: 181 VIPVPGPSAVVAAISASGLSTDEFTFVGFVPKHAASRRERLMVSASEEATQIFYVPPHKL 240
           V+P+PGPSA VAA+SASGLSTDEFTFVGF+PKH  SR++RLMVSA+E +TQ+FYVPPHKL
Sbjct: 181 VVPIPGPSAFVAALSASGLSTDEFTFVGFLPKHNGSRKDRLMVSANEASTQVFYVPPHKL 240

Query: 241 KQFLEETSQLFGESRQCVIAREMTKIHEEFWRGTLAEAKEFFSLHQTKGEITLLIEGNLN 300
           +QFLEETS LFG+SR+CVIARE+TK+HE+FWRGTL EAKE FS+HQ KGEITLLIEG  +
Sbjct: 241 RQFLEETSTLFGDSRRCVIAREITKLHEQFWRGTLEEAKEAFSVHQPKGEITLLIEGKTS 300

Query: 301 SEVETPSESQLEKELEELISSGHRLSTAVKLVASKTSARRKTVYSIALRRFGNQLGAEN- 360
             VETPSE +LE EL  LIS+GH LSTAVKLVA  TS +RKT+YSIALR+FG QLG E+ 
Sbjct: 301 PTVETPSECELENELSNLISNGHSLSTAVKLVAGGTSVKRKTIYSIALRKFGKQLGCEDI 360

Query: 361 --DSCK 364
              SCK
Sbjct: 361 DLGSCK 360

[Close]

BLAST of Bhi04M000183 vs. NCBI nr
Match: XP_022951884.1 (uncharacterized protein LOC111454620 [Cucurbita moschata])

HSP 1 Score: 644.4 bits (1661), Expect = 2.3e-181
Identity = 326/363 (89.81%), Postives = 343/363 (94.49%), Query Frame = 0


Query: 1   MLTRRIPSVAVYLPQALPWRRGPPHSINPRTPLEFHSFLAFCPKSCFVSFCSASQSISEA 60
           MLTRRIPSVAVYLPQALPWRRG PHSIN RTPLEFHS L+FCPK+C +SFCSASQ  SE 
Sbjct: 1   MLTRRIPSVAVYLPQALPWRRGLPHSINTRTPLEFHSLLSFCPKTCLLSFCSASQFSSEV 60

Query: 61  VEPIHAEESLKREPLKPGLYLVGTPIGNLEDITLRALRVLKSAHVILSEDTRHSGKLLQH 120
            EP H ++S KR PLKPGLY+V TPIGNLEDITLRALRVLKSA+VILSEDTRHSGKLLQH
Sbjct: 61  AEPTHLDDSSKRGPLKPGLYIVSTPIGNLEDITLRALRVLKSANVILSEDTRHSGKLLQH 120

Query: 121 FSIRTPLLSFHKFNESQREQTVVKRLKEGEIVALISDAGTPGISDPGTELVRLCVNKNIP 180
           FSI+TPLLSFHKFNESQREQTV+KRLKEGEIVALISDAGTPGISDPGTELVR CVN +IP
Sbjct: 121 FSIKTPLLSFHKFNESQREQTVLKRLKEGEIVALISDAGTPGISDPGTELVRQCVNASIP 180

Query: 181 VIPVPGPSAVVAAISASGLSTDEFTFVGFVPKHAASRRERLMVSASEEATQIFYVPPHKL 240
           V+P+PGPSAV+AA+SASGLSTDEFTFVGFVPKHAASRRERLMVSA+EEATQIFYVPPHK+
Sbjct: 181 VVPIPGPSAVIAALSASGLSTDEFTFVGFVPKHAASRRERLMVSANEEATQIFYVPPHKV 240

Query: 241 KQFLEETSQLFGESRQCVIAREMTKIHEEFWRGTLAEAKEFFSLHQTKGEITLLIEGNLN 300
           KQFLEETS LFGESRQCVIAREMTKIHEEFWRGTLAEAKE+FSLHQTKGEITLLIEG LN
Sbjct: 241 KQFLEETSSLFGESRQCVIAREMTKIHEEFWRGTLAEAKEYFSLHQTKGEITLLIEGKLN 300

Query: 301 SEVETPSESQLEKELEELISSGHRLSTAVKLVASKTSARRKTVYSIALRRFGNQLGAEND 360
           SEVETPSESQLEKELEELISSGHRLS AVKLVA KTSA RKTVYSIALRRFGNQLGAEND
Sbjct: 301 SEVETPSESQLEKELEELISSGHRLSMAVKLVAIKTSASRKTVYSIALRRFGNQLGAEND 360

Query: 361 SCK 364
           SCK
Sbjct: 361 SCK 363

[Close]

BLAST of Bhi04M000183 vs. NCBI nr
Match: XP_023538391.1 (uncharacterized protein LOC111799188 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 643.7 bits (1659), Expect = 3.9e-181
Identity = 324/363 (89.26%), Postives = 343/363 (94.49%), Query Frame = 0


Query: 1   MLTRRIPSVAVYLPQALPWRRGPPHSINPRTPLEFHSFLAFCPKSCFVSFCSASQSISEA 60
           MLTRRIPSVAVYLPQALPWRRGPPHSI  RTPLEFHS L+FCPK+C +SFCSASQ  SE 
Sbjct: 1   MLTRRIPSVAVYLPQALPWRRGPPHSIKARTPLEFHSLLSFCPKTCLLSFCSASQFSSEV 60

Query: 61  VEPIHAEESLKREPLKPGLYLVGTPIGNLEDITLRALRVLKSAHVILSEDTRHSGKLLQH 120
            EP H ++S KR PLKPGLY+V TPIGNLEDITLRALRVLKSA+VILSEDTRHSGKLLQH
Sbjct: 61  AEPTHMDDSSKRGPLKPGLYIVSTPIGNLEDITLRALRVLKSANVILSEDTRHSGKLLQH 120

Query: 121 FSIRTPLLSFHKFNESQREQTVVKRLKEGEIVALISDAGTPGISDPGTELVRLCVNKNIP 180
           FSI+TPLLSFHKFNESQREQTV+KRLKEGEIVALISDAGTPGISDPGTELVR CVN +IP
Sbjct: 121 FSIKTPLLSFHKFNESQREQTVLKRLKEGEIVALISDAGTPGISDPGTELVRQCVNASIP 180

Query: 181 VIPVPGPSAVVAAISASGLSTDEFTFVGFVPKHAASRRERLMVSASEEATQIFYVPPHKL 240
           V+P+PGPSAV+AA+SASGLSTDEFTFVGFVPKHA+SRRERLMVSA+EEATQIFYVPPHK+
Sbjct: 181 VVPIPGPSAVIAALSASGLSTDEFTFVGFVPKHASSRRERLMVSANEEATQIFYVPPHKV 240

Query: 241 KQFLEETSQLFGESRQCVIAREMTKIHEEFWRGTLAEAKEFFSLHQTKGEITLLIEGNLN 300
           KQFLEETS LFGESRQCVIAREMTKIHEEFWRGTLAEAKE+FSLHQTKGEITLLIEG LN
Sbjct: 241 KQFLEETSSLFGESRQCVIAREMTKIHEEFWRGTLAEAKEYFSLHQTKGEITLLIEGKLN 300

Query: 301 SEVETPSESQLEKELEELISSGHRLSTAVKLVASKTSARRKTVYSIALRRFGNQLGAEND 360
           SEVETPSESQLEKELEELISSGH+LS AVKLVA KTSA RKTVYSIALRRFGNQLGAEND
Sbjct: 301 SEVETPSESQLEKELEELISSGHKLSMAVKLVAIKTSASRKTVYSIALRRFGNQLGAEND 360

Query: 361 SCK 364
           SCK
Sbjct: 361 SCK 363

[Close]

BLAST of Bhi04M000183 vs. NCBI nr
Match: XP_023002398.1 (uncharacterized protein LOC111496252 [Cucurbita maxima])

HSP 1 Score: 639.0 bits (1647), Expect = 9.5e-180
Identity = 322/363 (88.71%), Postives = 342/363 (94.21%), Query Frame = 0


Query: 1   MLTRRIPSVAVYLPQALPWRRGPPHSINPRTPLEFHSFLAFCPKSCFVSFCSASQSISEA 60
           MLTRRIPSVAVYLPQALPWRRGPPHSIN RTPLEFHS L+FCPK+C +SFCSASQ  SE 
Sbjct: 1   MLTRRIPSVAVYLPQALPWRRGPPHSINTRTPLEFHSLLSFCPKTCLLSFCSASQFSSEV 60

Query: 61  VEPIHAEESLKREPLKPGLYLVGTPIGNLEDITLRALRVLKSAHVILSEDTRHSGKLLQH 120
            EP H ++S KR  LKPGLY+V TPIGNLEDITLRALRVLKSA+VILSEDTRHSGKLLQH
Sbjct: 61  AEPTHMDDSSKRGSLKPGLYIVSTPIGNLEDITLRALRVLKSANVILSEDTRHSGKLLQH 120

Query: 121 FSIRTPLLSFHKFNESQREQTVVKRLKEGEIVALISDAGTPGISDPGTELVRLCVNKNIP 180
           FSI+TPLLSFHKFNESQREQTV+KRLKEGEIVALISDAGTPGISDPGTELVRLCVN +IP
Sbjct: 121 FSIKTPLLSFHKFNESQREQTVLKRLKEGEIVALISDAGTPGISDPGTELVRLCVNASIP 180

Query: 181 VIPVPGPSAVVAAISASGLSTDEFTFVGFVPKHAASRRERLMVSASEEATQIFYVPPHKL 240
           V+P+PGPSAV+AA+SASGLST+EFTFVGFVPKHAASRR+RLMVSA+EEATQIFYVPPHK+
Sbjct: 181 VVPIPGPSAVIAALSASGLSTNEFTFVGFVPKHAASRRKRLMVSANEEATQIFYVPPHKV 240

Query: 241 KQFLEETSQLFGESRQCVIAREMTKIHEEFWRGTLAEAKEFFSLHQTKGEITLLIEGNLN 300
           KQFLEETS LFGESR+CVIAREMTKIHEEFWRGTLAEAKE+FSLHQTKGEITLLIEG LN
Sbjct: 241 KQFLEETSSLFGESRKCVIAREMTKIHEEFWRGTLAEAKEYFSLHQTKGEITLLIEGKLN 300

Query: 301 SEVETPSESQLEKELEELISSGHRLSTAVKLVASKTSARRKTVYSIALRRFGNQLGAEND 360
           SEVETPSESQLE ELEELISSGHRLS AVKLVA KTSA RKTVYSIALRRFGNQLG END
Sbjct: 301 SEVETPSESQLENELEELISSGHRLSMAVKLVAIKTSASRKTVYSIALRRFGNQLGTEND 360

Query: 361 SCK 364
           SCK
Sbjct: 361 SCK 363

[Close]

BLAST of Bhi04M000183 vs. NCBI nr
Match: XP_004152680.1 (PREDICTED: uncharacterized protein LOC101212996 [Cucumis sativus] >KGN62631.1 hypothetical protein Csa_2G362440 [Cucumis sativus])

HSP 1 Score: 634.8 bits (1636), Expect = 1.8e-178
Identity = 330/365 (90.41%), Postives = 341/365 (93.42%), Query Frame = 0


Query: 1   MLTRRIPSVAVYLPQALPWRRGPPH--SINPRTPLEFHSFLAFCPKSCFVSFCSASQSIS 60
           ML RRIPSVAVYLPQALPWRR PPH  SIN  T LEFHS LAFCPK+  VS CSASQ   
Sbjct: 1   MLARRIPSVAVYLPQALPWRRRPPHSYSINYGTALEFHSLLAFCPKTLLVSLCSASQLTY 60

Query: 61  EAVEPIHAEESLKREPLKPGLYLVGTPIGNLEDITLRALRVLKSAHVILSEDTRHSGKLL 120
           EAVEPIHAEES KR  LKPGLYLVGTPIGNLEDITLRALRVLKSAHVILSEDTRHSGKLL
Sbjct: 61  EAVEPIHAEESSKRGSLKPGLYLVGTPIGNLEDITLRALRVLKSAHVILSEDTRHSGKLL 120

Query: 121 QHFSIRTPLLSFHKFNESQREQTVVKRLKEGEIVALISDAGTPGISDPGTELVRLCVNKN 180
           QHFSI+TPLLSFHKFNESQREQTV+KRLK+GEIVALISDAGTPGISDPGTELVRLCVN+N
Sbjct: 121 QHFSIKTPLLSFHKFNESQREQTVLKRLKQGEIVALISDAGTPGISDPGTELVRLCVNEN 180

Query: 181 IPVIPVPGPSAVVAAISASGLSTDEFTFVGFVPKHAASRRERLMVSASEEATQIFYVPPH 240
           IPV+PVPGPSAVVAAISASGLSTDEFTFVGF+PKHAASRRERLMVSA+EEATQIFYVPPH
Sbjct: 181 IPVVPVPGPSAVVAAISASGLSTDEFTFVGFLPKHAASRRERLMVSANEEATQIFYVPPH 240

Query: 241 KLKQFLEETSQLFGESRQCVIAREMTKIHEEFWRGTLAEAKEFFSLHQTKGEITLLIEGN 300
           KLKQFLEETSQLFGESR+CVIAREMTKIHEEFWRGTLAEAKEFFSL+QTKGEITLLIEG 
Sbjct: 241 KLKQFLEETSQLFGESRRCVIAREMTKIHEEFWRGTLAEAKEFFSLNQTKGEITLLIEGK 300

Query: 301 LNSEVETPSESQLEKELEELISSGHRLSTAVKLVASKTSARRKTVYSIALRRFGNQLGAE 360
           L  EVETPSESQLEKELEELIS+GHRLS AVKLVASKTS  RKTVYSIALRRFGNQLG E
Sbjct: 301 LCPEVETPSESQLEKELEELISTGHRLSMAVKLVASKTSTSRKTVYSIALRRFGNQLGVE 360

Query: 361 NDSCK 364
           NDSCK
Sbjct: 361 NDSCK 365

[Close]

BLAST of Bhi04M000183 vs. NCBI nr
Match: XP_008444754.1 (PREDICTED: ribosomal RNA small subunit methyltransferase I [Cucumis melo] >XP_016900042.1 PREDICTED: ribosomal RNA small subunit methyltransferase I [Cucumis melo])

HSP 1 Score: 634.0 bits (1634), Expect = 3.1e-178
Identity = 327/365 (89.59%), Postives = 343/365 (93.97%), Query Frame = 0


Query: 1   MLTRRIPSVAVYLPQALPWRRGPP--HSINPRTPLEFHSFLAFCPKSCFVSFCSASQSIS 60
           ML RR+PSVAVYLPQALPWRR PP  HSIN RT L FHS LAFCPK+  VS CSASQ  +
Sbjct: 1   MLARRMPSVAVYLPQALPWRRRPPHSHSINSRTALVFHSLLAFCPKTILVSLCSASQLAT 60

Query: 61  EAVEPIHAEESLKREPLKPGLYLVGTPIGNLEDITLRALRVLKSAHVILSEDTRHSGKLL 120
           EAVEPIHAEE  KR PLKPGLYLVGTPIGNLEDITLRALRVLKSAHVILSEDTRHSGKLL
Sbjct: 61  EAVEPIHAEEFSKRGPLKPGLYLVGTPIGNLEDITLRALRVLKSAHVILSEDTRHSGKLL 120

Query: 121 QHFSIRTPLLSFHKFNESQREQTVVKRLKEGEIVALISDAGTPGISDPGTELVRLCVNKN 180
           QHFSI+TPLLSFHKFNESQREQTV+KRLK+GEIVALISDAGTPGISDPGTELVRLCVN+N
Sbjct: 121 QHFSIKTPLLSFHKFNESQREQTVLKRLKQGEIVALISDAGTPGISDPGTELVRLCVNEN 180

Query: 181 IPVIPVPGPSAVVAAISASGLSTDEFTFVGFVPKHAASRRERLMVSASEEATQIFYVPPH 240
           IPV+PVPGPSAVVAAISASGLSTDEFTFVGF+PKHAA+RRERLMVSA+EEATQIFYVPPH
Sbjct: 181 IPVVPVPGPSAVVAAISASGLSTDEFTFVGFLPKHAATRRERLMVSANEEATQIFYVPPH 240

Query: 241 KLKQFLEETSQLFGESRQCVIAREMTKIHEEFWRGTLAEAKEFFSLHQTKGEITLLIEGN 300
           KLKQFLEETSQLFGESR+CVIAREMTKIHEEFWRGTLAEAKEFFSL+ TKGEITLLIEG 
Sbjct: 241 KLKQFLEETSQLFGESRRCVIAREMTKIHEEFWRGTLAEAKEFFSLNPTKGEITLLIEGK 300

Query: 301 LNSEVETPSESQLEKELEELISSGHRLSTAVKLVASKTSARRKTVYSIALRRFGNQLGAE 360
           L+ EVETPSESQLEKELEELIS+GHRLS AVKLVASKTSA RKTVYSIALRRFGNQLG E
Sbjct: 301 LSPEVETPSESQLEKELEELISTGHRLSMAVKLVASKTSASRKTVYSIALRRFGNQLGVE 360

Query: 361 NDSCK 364
           N+SCK
Sbjct: 361 NNSCK 365

The following BLAST results are available for this feature:

BLAST of Bhi04M000183 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|P74038\|RSMI_SYNY3	4.7e-61	45.93	Ribosomal RNA small subunit methyltransferase I OS=Synechocystis sp. (strain PCC...	[more]
sp\|Q9KGL2\|RSMI_BACHD	3.8e-55	43.51	Ribosomal RNA small subunit methyltransferase I OS=Bacillus halodurans (strain A...	[more]
sp\|P45298\|RSMI_HAEIN	1.5e-51	41.54	Ribosomal RNA small subunit methyltransferase I OS=Haemophilus influenzae (strai...	[more]
sp\|Q9CN04\|RSMI_PASMU	5.7e-51	40.73	Ribosomal RNA small subunit methyltransferase I OS=Pasteurella multocida (strain...	[more]
sp\|Q9HVZ3\|RSMI_PSEAE	9.7e-51	43.84	Ribosomal RNA small subunit methyltransferase I OS=Pseudomonas aeruginosa (strai...	[more]

BLAST of Bhi04M000183 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G45110.1	2.7e-120	69.01	Tetrapyrrole (Corrin/Porphyrin) Methylases	[more]

BLAST of Bhi04M000183 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0LRJ7\|A0A0A0LRJ7_CUCSA	1.2e-178	90.41	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G362440 PE=3 SV=1	[more]
tr\|A0A1S3BBU6\|A0A1S3BBU6_CUCME	2.0e-178	89.59	ribosomal RNA small subunit methyltransferase I OS=Cucumis melo OX=3656 GN=LOC10...	[more]
tr\|A0A2P5DMT6\|A0A2P5DMT6_PARAD	1.1e-136	70.49	rRNA small subunit methyltransferase I OS=Parasponia andersonii OX=3476 GN=PanWU...	[more]
tr\|A0A2C9VZ05\|A0A2C9VZ05_MANES	1.1e-134	70.91	Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_05G154300 PE=3 SV=...	[more]
tr\|A0A2P5EZL8\|A0A2P5EZL8_9ROSA	3.1e-134	69.67	rRNA small subunit methyltransferase I OS=Trema orientalis OX=63057 GN=TorRG33x0...	[more]

BLAST of Bhi04M000183 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022951884.1	2.3e-181	89.81	uncharacterized protein LOC111454620 [Cucurbita moschata]	[more]
XP_023538391.1	3.9e-181	89.26	uncharacterized protein LOC111799188 [Cucurbita pepo subsp. pepo]	[more]
XP_023002398.1	9.5e-180	88.71	uncharacterized protein LOC111496252 [Cucurbita maxima]	[more]
XP_004152680.1	1.8e-178	90.41	PREDICTED: uncharacterized protein LOC101212996 [Cucumis sativus] >KGN62631.1 hy...	[more]
XP_008444754.1	3.1e-178	89.59	PREDICTED: ribosomal RNA small subunit methyltransferase I [Cucumis melo] >XP_01...	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **Biological Process**
Term	Definition
GO:0008152	metabolic process

Vocabulary: **Molecular Function**
Term	Definition
GO:0008168	methyltransferase activity

Vocabulary: **INTERPRO**
Term	Definition
IPR035996	4pyrrol_Methylase_sf
IPR018063	SAM_MeTrfase_RsmI_CS
IPR000878	4pyrrol_Mease
IPR008189	rRNA_ssu_MeTfrase_I
IPR014776	4pyrrole_Mease_sub2
IPR014777	4pyrrole_Mease_sub1

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008152	metabolic process
biological_process	GO:0032259	methylation
molecular_function	GO:0008168	methyltransferase activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Bhi04G000183	Bhi04G000183	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Bhi04M000183	Bhi04M000183-protein	polypeptide

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Bhi04M000183.utr5p1	Bhi04M000183.utr5p1	five_prime_UTR

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Bhi04M000183.exon1	Bhi04M000183.exon1	exon
Bhi04M000183.exon2	Bhi04M000183.exon2	exon
Bhi04M000183.exon3	Bhi04M000183.exon3	exon
Bhi04M000183.exon4	Bhi04M000183.exon4	exon
Bhi04M000183.exon5	Bhi04M000183.exon5	exon
Bhi04M000183.exon6	Bhi04M000183.exon6	exon
Bhi04M000183.exon7	Bhi04M000183.exon7	exon
Bhi04M000183.exon8	Bhi04M000183.exon8	exon
Bhi04M000183.exon9	Bhi04M000183.exon9	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.Bhi04M000183	cds.Bhi04M000183	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Bhi04M000183.utr3p1	Bhi04M000183.utr3p1	three_prime_UTR

InterPro

Analysis Name: InterPro Annotations of wax gourd
Date Performed: 2019-11-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR014777	Tetrapyrrole methylase, subdomain 1	GENE3D	G3DSA:3.40.1010.10		coord: 64..185 e-value: 6.4E-45 score: 153.8
IPR014776	Tetrapyrrole methylase, subdomain 2	GENE3D	G3DSA:3.30.950.10		coord: 186..301 e-value: 4.4E-38 score: 131.7
IPR008189	rRNA small subunit methyltransferase I	TIGRFAM	TIGR00096	TIGR00096	coord: 79..348 e-value: 5.4E-80 score: 266.7
IPR008189	rRNA small subunit methyltransferase I	PANTHER	PTHR21091:SF18	RIBOSOMAL RNA SMALL SUBUNIT METHYLTRANSFERASE I	coord: 68..351
IPR008189	rRNA small subunit methyltransferase I	HAMAP	MF_01877	16SrRNA_methyltr_I	coord: 78..297 score: 35.805
IPR008189	rRNA small subunit methyltransferase I	CDD	cd11648	RsmI	coord: 79..297 e-value: 1.07883E-126 score: 365.164
IPR000878	Tetrapyrrole methylase	PFAM	PF00590	TP_methylase	coord: 79..278 e-value: 2.2E-29 score: 102.8
None	No IPR available	PANTHER	PTHR21091	METHYLTETRAHYDROFOLATE:HOMOCYSTEINE METHYLTRANSFERASE RELATED	coord: 68..351
IPR018063	SAM-dependent methyltransferase RsmI, conserved site	PROSITE	PS01296	RSMI	coord: 156..167
IPR035996	Tetrapyrrole methylase superfamily	SUPERFAMILY	SSF53790	Tetrapyrrole methylase	coord: 77..295