Bhi01M000305 (mRNA) Wax gourd

BLAST of Bhi01M000305 vs. TAIR10
Match: AT1G64300.1 (Protein kinase family protein)

HSP 1 Score: 714.9 bits (1844), Expect = 4.6e-206
Identity = 373/721 (51.73%), Postives = 480/721 (66.57%), Query Frame = 0

Query: 1   MEQFRQLGEALGSVKALMLFKDSVHLNQRQCCLLLDFLSFAYDSVAEEMKQNLRFEEKHT 60
           MEQFRQ+GE LGS+ ALM+ +D + +NQRQCCLLLD  S  +++VAEE++ NL+ EEKHT
Sbjct: 1   MEQFRQIGEVLGSLNALMVLQDDILINQRQCCLLLDIFSLGFNTVAEEIRHNLKLEEKHT 60

Query: 61  RWKVLDQPLRDLNRVFKEAEWYIRQCLETKDWWAKAIMLYQNTDCIELHIHNLLYSITVV 120
           +W+ L+QPLR+L RVFKE E Y+R C+  KDWW K I  +QN DC+E HIHNLL   + V
Sbjct: 61  KWRALEQPLRELYRVFKEGEMYVRNCMSNKDWWGKVINFHQNKDCVEFHIHNLLCYFSAV 120

Query: 121 VEAIETAGENSGSDHDDMLKKRLINSIKYRREHKDLKIFKWKFGKQYLITQDFCNRMDAV 180
           +EAIETAGE SG D  +M ++R++ S KY RE  D K+F+W+FGKQYL+ +D C R +  
Sbjct: 121 IEAIETAGEISGLDPSEMERRRVVFSRKYDREWNDPKLFQWRFGKQYLVPRDICLRFEHS 180

Query: 181 WNEDRWVLLNKIREKKFTAS---SKYEQRLTDLLLKNLDGPESLNGKLLPSSMLVGSRDY 240
           W EDRW L+  ++EK+ + S    K E+RL D LLK L G E  NGKL PSS+LVGS+DY
Sbjct: 181 WREDRWNLVEALQEKRKSKSDEIGKTEKRLADFLLKKLTGLEQFNGKLFPSSILVGSKDY 240

Query: 241 QVRRRLGGGSQYKEILWLGESFAMRHFFGEIESLIPEISMLLSLSHPNITRFLCGFTDEE 300
           QVRRRLGGG QYKEI WLG+SF +RHFFG++E L  EIS LLSL H NI ++LCGF DEE
Sbjct: 241 QVRRRLGGGGQYKEIQWLGDSFVLRHFFGDLEPLNAEISSLLSLCHSNILQYLCGFYDEE 300

Query: 301 KKECFLIMELMSRDLSSYVKEICGPRKRMPFPLPVALDLMLQIARGMEYLHSKKIYHGDL 360
           +KEC L+MELM +DL SY+KE CGPR+R  F +PV +D+MLQIARGMEYLHS +I+HGDL
Sbjct: 301 RKECSLVMELMHKDLKSYMKENCGPRRRYLFSVPVVIDIMLQIARGMEYLHSNEIFHGDL 360

Query: 361 NPCNILVKPRAYSTDGYVHAQVSGFGLPVVKFKN----SSNQNESLPFIWYAPEVLE--E 420
           NP NIL+K R++ T+GY HA++SGFGL  VK ++    SS      P IWYAPEVL   E
Sbjct: 361 NPMNILLKERSH-TEGYFHAKISGFGLTSVKNQSFSRASSRPTTPDPVIWYAPEVLAEME 420

Query: 421 QDQSGSAESSKYTEKSDVYSFGMVCFEVLTGKVPFEDSHLQGDKMSRNIRAGERPLFPHS 480
           QD  G+A  SK+T K+DVYSF MVCFE++TGKVPFED HLQGDKM++NIR GERPLFP  
Sbjct: 421 QDLKGTAPRSKFTHKADVYSFAMVCFELITGKVPFEDDHLQGDKMAKNIRTGERPLFPFP 480

Query: 481 MPKYVTNLTKRCWHTDPNQRPSFPSICRILRYTKRFVAMNPDYNSQTDPAMPTVDYCDIE 540
            PKY+ +L KRCWH++P+QRP+F SICRILRY K+F+ +NPD+        P VD  D+E
Sbjct: 481 SPKYLVSLIKRCWHSEPSQRPTFSSICRILRYIKKFLVVNPDH-GHLQIQNPLVDCWDLE 540

Query: 541 SGLLRRLPSCGISEASSAITDIPFQMFAYRIVEKEKAGANIKDTSESGSDASACGDETTS 600
           +  L++      S A S +T IPFQ+++YRI EKEK   N     E  SDA     E+ S
Sbjct: 541 ARFLKKFSMESGSHAGS-VTQIPFQLYSYRIAEKEKMSPNF--NKEENSDAGESVSESVS 600

Query: 601 SVDDPFPTL--------------------VEKKLPVSREGTTRRLSLTKNNSDIKLNKL- 660
            V++P  T+                    V  + P+ +    ++   T  N    ++ L 
Sbjct: 601 VVEEPPTTMPMYTKSLCLDAISEYSDTRSVYSEAPIKKTSALKKSGDTIKNRRNSISGLR 660

Query: 661 -PGT----PKGRFSRPPQISPRGRSMRMNSESQLMAMSP--------KVRRLSGHASDSE 679
            PG+    P+        +SP GRS                      + R+L+G ASDSE
Sbjct: 661 SPGSSPIKPRSTPKVSSPLSPFGRSSSXXXXXXXXXXXXXXXXXSHGRRRQLTGPASDSE 716

BLAST of Bhi01M000305 vs. TAIR10
Match: AT5G41730.1 (Protein kinase family protein)

HSP 1 Score: 712.6 bits (1838), Expect = 2.3e-205
Identity = 368/719 (51.18%), Postives = 490/719 (68.15%), Query Frame = 0

Query: 1   MEQFRQLGEALGSVKALMLFKDSVHLNQRQCCLLLDFLSFAYDSVAEEMKQNLRFEEKHT 60
           MEQFRQ+GE LGS+ ALM+ +D + +NQRQCCLLL+  S A+++VAEE++QNL+ EEKHT
Sbjct: 1   MEQFRQIGEVLGSLNALMVLQDDILINQRQCCLLLELFSLAFNTVAEEIRQNLKLEEKHT 60

Query: 61  RWKVLDQPLRDLNRVFKEAEWYIRQCLETKDWWAKAIMLYQNTDCIELHIHNLLYSITVV 120
           +W+ L+QPLR+L RVFKE E Y++ C++  DWW K I L+QN DC+E HIHNL    + V
Sbjct: 61  KWRALEQPLRELYRVFKEGELYVKHCMDNSDWWGKVINLHQNKDCVEFHIHNLFCYFSAV 120

Query: 121 VEAIETAGENSGSDHDDMLKKRLINSIKYRREHKDLKIFKWKFGKQYLITQDFCNRMDAV 180
           VEAIE AGE SG D  +M ++R++ S KY RE  D K+F+W+FGKQYL+++D C+R +  
Sbjct: 121 VEAIEAAGEISGLDPSEMERRRVVFSRKYDREWNDPKMFQWRFGKQYLLSRDICSRFEHS 180

Query: 181 WNEDRWVLLNKIREKKFTAS---SKYEQRLTDLLLKNLDGPESLNGKLLPSSMLVGSRDY 240
           W EDRW L+  ++EK+ + S    K E+RL DLLLK L G E  NGKL PSS+L+GS+DY
Sbjct: 181 WREDRWNLVEALQEKRKSDSDDIGKTEKRLADLLLKKLTGLEQFNGKLFPSSILLGSKDY 240

Query: 241 QVRRRLGGGSQYKEILWLGESFAMRHFFGEIESLIPEISMLLSLSHPNITRFLCGFTDEE 300
           QV++RL    QYKEI WLG+SFA+RHFF ++E L  EIS LL+L H NI ++LCGF DEE
Sbjct: 241 QVKKRLDADGQYKEIQWLGDSFALRHFFSDLEPLSSEISSLLALCHSNILQYLCGFYDEE 300

Query: 301 KKECFLIMELMSRDLSSYVKEICGPRKRMPFPLPVALDLMLQIARGMEYLHSKKIYHGDL 360
           +KECFL+MELM +DL SY+KE CGPR+R  F +PV +D+MLQIARGMEYLH   I+HGDL
Sbjct: 301 RKECFLVMELMHKDLQSYMKENCGPRRRYLFSIPVVIDIMLQIARGMEYLHGNDIFHGDL 360

Query: 361 NPCNILVKPRAYSTDGYVHAQVSGFGL-PVVKFKNSSNQNESLPFIWYAPEVLE--EQDQ 420
           NP NI +K R++ T+GY HA++ GFGL  VVK ++SS      P IWYAPEVL   EQD 
Sbjct: 361 NPMNIHLKERSH-TEGYFHAKICGFGLSSVVKAQSSSKPGTPDPVIWYAPEVLAEMEQDL 420

Query: 421 SGSAESSKYTEKSDVYSFGMVCFEVLTGKVPFEDSHLQGDKMSRNIRAGERPLFPHSMPK 480
           +G    SK T K+DVYSF MVCFE++TGKVPFEDSHLQG+ M+ NIR GERPLFP   PK
Sbjct: 421 NGKTPKSKLTHKADVYSFAMVCFELITGKVPFEDSHLQGEPMTINIRMGERPLFPFPSPK 480

Query: 481 YVTNLTKRCWHTDPNQRPSFPSICRILRYTKRFVAMNPDYNSQTDPAMPTVDYCDIESGL 540
           Y+ +L KRCWH++P+QRP+F SICRILRY K+F+ +NPD+        P VD  D+E+  
Sbjct: 481 YLVSLIKRCWHSEPSQRPNFSSICRILRYIKKFLVVNPDH-GHPQMQTPLVDCWDLEARF 540

Query: 541 LRRLPSCGISEASSAITDIPFQMFAYRIVEKEKAGANIKDTSESGSDASACGDETTSSVD 600
           LR+ P    S  +S +  IPFQ+++YR++EKEK   N K++SE+         E+ S V+
Sbjct: 541 LRKFPGDAGSHTAS-VNQIPFQLYSYRVLEKEKMNPNSKESSETSE------SESVSVVE 600

Query: 601 DPFPTLVEKKL------PVSREGTTRRL----------SLTKNNSDIKLNKLP------- 660
           DP   ++ +         +S    TR +          +L K+    KL + P       
Sbjct: 601 DPPNAMITRDTKSLCLDTISEYSDTRSVYSEAPMKKVSALKKSGEMAKLRRSPSLGSEKL 660

Query: 661 -----GTPKGRFS-RPPQISPRGRSMRMNSESQL-----MAMSPKVRR-LSGHASDSEL 679
                 T K R S +   +SP GRS++   +++L       ++P +RR  +GHASDSEL
Sbjct: 661 RSAGTSTVKARSSPKVSPLSPFGRSIKARKDNRLPLSPMSPLTPGIRRQQTGHASDSEL 710

BLAST of Bhi01M000305 vs. TAIR10
Match: AT1G01450.1 (Protein kinase superfamily protein)

HSP 1 Score: 512.7 bits (1319), Expect = 3.5e-145
Identity = 266/443 (60.05%), Postives = 340/443 (76.75%), Query Frame = 0

Query: 207 LTDLLLKNL-DGPESLNGKLLPSSMLVGSRDYQVRRRLGGGSQYKEILWLGESFAMRHFF 266
           + D LLK+L DG ES   KL PSS+L  ++DYQV++RLG GSQYKEI WLGESFA+RHFF
Sbjct: 1   MADFLLKHLGDGNES--PKLFPSSLLDNTKDYQVKKRLGNGSQYKEITWLGESFALRHFF 60

Query: 267 GEIESLIPEISMLLSLSHPNITRFLCGFTDEEKKECFLIMELMSRDLSSYVKEICGPRKR 326
           G+I++L+P+I+ LLSLSHPNI  +LCGFTDEEKKECFL+MELM + L  ++KE+CGPRK+
Sbjct: 61  GDIDALLPQITPLLSLSHPNIVYYLCGFTDEEKKECFLVMELMRKTLGMHIKEVCGPRKK 120

Query: 327 MPFPLPVALDLMLQIARGMEYLHSKKIYHGDLNPCNILVKPRA-YSTDGYVHAQVSGFGL 386
               LPVA+DLMLQIA GMEYLHSK+IYHG+LNP NILVKPR+  S DGY+  ++ GFGL
Sbjct: 121 NTLSLPVAVDLMLQIALGMEYLHSKRIYHGELNPSNILVKPRSNQSGDGYLLGKIFGFGL 180

Query: 387 PVVKFKNS-----SNQNESLPFIWYAPEVLEEQDQSGSAESSKYTEKSDVYSFGMVCFEV 446
             VK  +S     ++QNE+ PFIWY+PEVLEEQ+QSG+A S KY++KSDVYSFGMV FE+
Sbjct: 181 NSVKGFSSKSASLTSQNENFPFIWYSPEVLEEQEQSGTAGSLKYSDKSDVYSFGMVSFEL 240

Query: 447 LTGKVPFEDSHLQGDKMSRNIRAGERPLFPHSMPKYVTNLTKRCWHTDPNQRPSFPSICR 506
           LTGKVPFEDSHLQGDKMSRNIRAGERPLFP + PK++TNLTKRCWH DPNQRP+F SI R
Sbjct: 241 LTGKVPFEDSHLQGDKMSRNIRAGERPLFPFNSPKFITNLTKRCWHADPNQRPTFSSISR 300

Query: 507 ILRYTKRFVAMNPD--YNSQTDPAM-PTVDYCDIESGLLRRLPSCGISEASSAITDIPFQ 566
           ILRY KRF+A+NP+   +SQ DP++ PTVDYC+IE+ LL++L     S   + ++ +PFQ
Sbjct: 301 ILRYIKRFLALNPECYSSSQQDPSIAPTVDYCEIETKLLQKL--SWESTELTKVSQVPFQ 360

Query: 567 MFAYRIVEKEKA--GANIKDTSESGSDASACG-DETTSSVDDPFPTLVEKKLPVSRE--G 626
           MFAYR+VE+ K     N+++ SESGS+ ++C  DE  +  D+      E++L  S    G
Sbjct: 361 MFAYRVVERAKTCEKDNLREPSESGSEWASCSEDEGGAGSDEQLSYAKERRLSCSSNDVG 420

Query: 627 TTRR--LSLTKNNSDIKLNKLPG 633
            +++   +L K  S +K  + PG
Sbjct: 421 MSKKQVSNLLKRASSLKPIQKPG 439

BLAST of Bhi01M000305 vs. TAIR10
Match: AT3G46930.1 (Protein kinase superfamily protein)

HSP 1 Score: 119.4 bits (298), Expect = 8.6e-27
Identity = 72/260 (27.69%), Postives = 121/260 (46.54%), Query Frame = 0

Query: 269 ESLIPEISMLLSLSHPNITRFLCGFTDEEKKECFLIMELMSRDLSSYVKEICGPRKRMPF 328
           +  I E ++L  LSHPN+ +F+   T      C +   +    L SY+ ++    ++   
Sbjct: 207 KEFIVEATLLSRLSHPNVVKFVGVNTG----NCIITEYVPRGSLRSYLHKL----EQKSL 266

Query: 329 PLPVALDLMLQIARGMEYLHSKKIYHGDLNPCNILVKPRAYSTDGYVHAQVSGFGLPVVK 388
           PL   +D  L IA+GMEY+HS++I H DL P N+L+       D   H +++ FG+   +
Sbjct: 267 PLEQLIDFGLDIAKGMEYIHSREIVHQDLKPENVLI-------DNDFHLKIADFGIACEE 326

Query: 389 FKNSSNQNESLPFIWYAPEVLEEQDQSGSAESSKYTEKSDVYSFGMVCFEVLTGKVPFED 448
                  +    + W APEVL+            +  K DVYSFG++ +E++ G +P+E+
Sbjct: 327 EYCDVLGDNIGTYRWMAPEVLKR---------IPHGRKCDVYSFGLLLWEMVAGALPYEE 386

Query: 449 SHLQGDKMSRNIRAGERPLFPHSMPKYVTNLTKRCWHTDPNQRPSFPSICRILRYTKRFV 508
                      I    RP+ P   P  +  L +RCW +  ++RP F  I ++L + K+ +
Sbjct: 387 MKFAEQIAYAVIYKKIRPVIPTDCPAAMKELIERCWSSQTDKRPEFWQIVKVLEHFKKSL 442

Query: 509 AMNPDYNSQTDPAMPTVDYC 529
                 N       P +  C
Sbjct: 447 TSEGKLNLLPSQICPELKKC 442

BLAST of Bhi01M000305 vs. TAIR10
Match: AT5G58950.1 (Protein kinase superfamily protein)

HSP 1 Score: 116.7 bits (291), Expect = 5.6e-26
Identity = 70/231 (30.30%), Postives = 120/231 (51.95%), Query Frame = 0

Query: 274 EISMLLSLSHPNITRFLCGFTDEEKKECFLIMELMSRDLSSYVKEICGPRKRMPFPLPVA 333
           E+++L  L+HPN+ +F+  + D     C L   L    L S++ +   P  R   PL   
Sbjct: 258 EVTLLSRLTHPNVIKFVGAYKD-PPVYCVLTQYLPEGSLRSFLHK---PENR-SLPLKKL 317

Query: 334 LDLMLQIARGMEYLHSKKIYHGDLNPCNILVKPRAYSTDGYVHAQVSGFGLPVVKFKNSS 393
           ++  + IARGMEY+HS++I H DL P N+L+       D   H +++ FG+   +     
Sbjct: 318 IEFAIDIARGMEYIHSRRIIHRDLKPENVLI-------DEEFHLKIADFGIACEEEYCDM 377

Query: 394 NQNESLPFIWYAPEVLEEQDQSGSAESSKYTEKSDVYSFGMVCFEVLTGKVPFEDSH-LQ 453
             ++   + W APE+++ +          +  K+DVYSFG+V +E++ G +P+ED + +Q
Sbjct: 378 LADDPGTYRWMAPEMIKRK---------PHGRKADVYSFGLVLWEMVAGAIPYEDMNPIQ 437

Query: 454 G--DKMSRNIRAGERPLFPHSMPKYVTNLTKRCWHTDPNQRPSFPSICRIL 502
                + +NI    RP  P   P  +  L ++CW   P++RP F  I ++L
Sbjct: 438 AAFAVVHKNI----RPAIPGDCPVAMKALIEQCWSVAPDKRPEFWQIVKVL 463

BLAST of Bhi01M000305 vs. Swiss-Prot
Match: sp|P25848|PHY1_CERPU (Light-sensor Protein kinase OS=Ceratodon purpureus OX=3225 GN=PHY1 PE=3 SV=3)

HSP 1 Score: 173.7 bits (439), Expect = 6.9e-42
Identity = 113/306 (36.93%), Postives = 156/306 (50.98%), Query Frame = 0

Query: 238  QVRRRLGGGSQ--YKEILWLGESFAMRHFFG-EIESLIPEISMLLSLSHPNITRFLCGFT 297
            Q+   LG GS    ++ +WLG   A + F+G   E    E+ +L  L HPNIT   C  +
Sbjct: 1005 QITGSLGSGSSATVEKAVWLGTPVAKKTFYGRNNEDFKREVEILAELCHPNITSMFC--S 1064

Query: 298  DEEKKECFLIMELMSRDLSSYVKEICGPRK---RMPFPLPVALDLMLQIARGMEYLHSKK 357
               +++C +IMELM  DL + ++      +     PF +   +D++LQ + GM YLH K 
Sbjct: 1065 PLYRRKCSIIMELMDGDLLALMQRRLDRNEDHDSPPFSILEVVDIILQTSEGMNYLHEKG 1124

Query: 358  IYHGDLNPCNILVK--PRAYSTDGYVHAQVSGFGLPVVKFKNSSNQNESL---PFIWYAP 417
            I H DL   NILVK      S  GYVH +V+ FGL   K  ++   N++       W AP
Sbjct: 1125 IIHRDLKSMNILVKSVKVTKSEIGYVHVKVADFGLSKTKDSSTRYSNQTWNRGTNRWMAP 1184

Query: 418  EVLEEQDQSGSAESS------KYTEKSDVYSFGMVCFEVLTGKVPFEDSHLQGDKMSRNI 477
            EV+    +S   E S      KY  KSDVYSFGMVC+EVLTG VPF +     + + R +
Sbjct: 1185 EVINLGYESTEGEISFDGKVPKYPLKSDVYSFGMVCYEVLTGDVPFPEEK-NPNNVKRMV 1244

Query: 478  RAGERPLFPHSMPKYVTNLTKRCWHTDPNQRPSFPSICRILRYTK-----RFVAMNPDYN 522
              G RP  P   P  +  L   CW+ DP +RPSF  IC+ L+Y K      F +    Y 
Sbjct: 1245 LEGVRPDLPAHCPIELKALITDCWNQDPLKRPSFAVICQKLKYLKYLLMTGFSSYQDSYP 1304

BLAST of Bhi01M000305 vs. Swiss-Prot
Match: sp|Q54TM7|DRKD_DICDI (Probable serine/threonine-protein kinase drkD OS=Dictyostelium discoideum OX=44689 GN=drkD PE=2 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 1.2e-25
Identity = 91/266 (34.21%), Postives = 122/266 (45.86%), Query Frame = 0

Query: 244  GGGSQYKEILWLGESFAMRHFFGE------IESLIPEISMLLSLSHPNITRFLCGFTDEE 303
            GG  Q     W G   A++  F +      I  L  E+ +L  L HPNI  F+ G   E 
Sbjct: 860  GGYGQVFRGSWRGTEVAVKMLFNDNVNLKLISDLRKEVDLLCKLRHPNIVLFM-GACTEP 919

Query: 304  KKECFLIMELMSRDLSSYVKEICGPRKRMPFPLPVALDLMLQIARGMEYLHSKK--IYHG 363
               C ++ E +SR   S    +      M + L   L L    ARGM YLHS+   I H 
Sbjct: 920  SSPC-IVTEYLSR--GSLANILLDESIEMDWGL--RLQLGFDCARGMTYLHSRNPIIIHR 979

Query: 364  DLNPCNILVKPRAYSTDGYVHAQVSGFGLPVVKFKNSSNQNESLPFIWYAPEVLEEQDQS 423
            DL   N+LV       D     +V+ FGL  VK  ++  +       W APEVL E+   
Sbjct: 980  DLKTDNLLV-------DDSWQVKVADFGLATVK-SHTFAKTMCGTTGWVAPEVLAEEG-- 1039

Query: 424  GSAESSKYTEKSDVYSFGMVCFEVLTGKVPFEDSHLQGDKMSRNIRAGERPLFPHSMPKY 483
                   YTEK+DVYS+ +V +E+LT  +P+   +    ++ R+I  GER   P   P  
Sbjct: 1040 -------YTEKADVYSYAIVLWELLTRLIPYAGKNTM--QVVRSIDRGERLPMPAWCPPK 1099

Query: 484  VTNLTKRCWHTDPNQRPSFPSICRIL 502
               L  RCW TDP  RPSFP I  I+
Sbjct: 1100 YAALMNRCWETDPTHRPSFPEILPIM 1100

BLAST of Bhi01M000305 vs. Swiss-Prot
Match: sp|Q55GU0|Y9955_DICDI (Probable serine/threonine-protein kinase DDB_G0267514 OS=Dictyostelium discoideum OX=44689 GN=DDB_G0267514 PE=3 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 3.5e-25
Identity = 82/285 (28.77%), Postives = 139/285 (48.77%), Query Frame = 0

Query: 228 SSMLVGSRDYQVRRRLGGGS---QYKEILWLGESFAMRHF-FGE------IESLIPEISM 287
           S + +   + ++  +LG G+    YK  LW G S A++     E      +E    E+++
Sbjct: 653 SDIEISFSELKISSKLGEGTFGVVYKG-LWRGSSVAIKQIKINEDVNNQVLEEFRKELTI 712

Query: 288 LLSLSHPNITRFLCGFTDEEKKECFLIMELMSRDLSSYVKEICGPRKRMPFPLPVALDLM 347
           L  L HPNI   +   T      CF+   L    L   +       K++   + +   L 
Sbjct: 713 LSRLRHPNIVLLMAACT-APPNLCFITEYLPGGSLYDALHS-----KKIKMNMQLYKKLA 772

Query: 348 LQIARGMEYLHSKKIYHGDLNPCNILVKPRAYSTDGYVHAQVSGFGLPVVKFKNSSNQNE 407
           +QIA+GM YLH   + H D+   N+L+       D +++ ++  FGL  +K K++     
Sbjct: 773 IQIAQGMNYLHLSGVIHRDIKSLNLLL-------DEHMNVKICDFGLSKLKSKSTEMTKS 832

Query: 408 SLPFIWYAPEVLEEQDQSGSAESSKYTEKSDVYSFGMVCFEVLTGKVPFEDSHLQGDKMS 467
               IW +PE+L  +D         YTEK DVY+FG++ +E+ TG++P+  S L   +++
Sbjct: 833 IGSPIWMSPELLMGED---------YTEKVDVYAFGIILWELGTGELPY--SGLDSVQLA 892

Query: 468 RNIRAGE-RPLFPHSMPKYVTNLTKRCWHTDPNQRPSFPSICRIL 502
             +     RP  P++ P  +++L + CWH DP +RPSF  I  +L
Sbjct: 893 LAVTTKSLRPPIPNAWPYQLSHLIQACWHQDPLKRPSFTEILNLL 912

BLAST of Bhi01M000305 vs. Swiss-Prot
Match: sp|Q7T6Y2|YR831_MIMIV (Putative serine/threonine-protein kinase/receptor R831 OS=Acanthamoeba polyphaga mimivirus OX=212035 GN=MIMI_R831 PE=3 SV=2)

HSP 1 Score: 115.9 bits (289), Expect = 1.7e-24
Identity = 79/263 (30.04%), Postives = 126/263 (47.91%), Query Frame = 0

Query: 254  WLGESFAMRHFF------GEIESLIPEISMLLSLSHPNITRFLCGFTDEEKKECFLIMEL 313
            W G   A++ F        ++     E++ L  L H NI  F+ G   ++   C +   +
Sbjct: 1383 WKGVDVAVKKFVKQKLSETQLLEFRAEMAFLSELKHSNIVTFI-GACIKKPNICIVTEYM 1442

Query: 314  MSRDLSSYVKEICGPRKRMPFPLPVALDLMLQIARGMEYLHSKK--IYHGDLNPCNILVK 373
               +L   +K    P  ++ F     L L+   A G++YLHS    I H D+ P NILV 
Sbjct: 1443 RMGNLRDVLK---NPDIKITF--ANKLKLLYGAAMGIDYLHSSNPMIVHRDIKPANILV- 1502

Query: 374  PRAYSTDGYVHAQVSGFGLPVVKFKNSSNQNESLPFIWYAPEVLEEQDQSGSAESSKYTE 433
                  D + + +++ FG   +K  N++      P  W APEV+            KY E
Sbjct: 1503 ------DEHFNVKIADFGFARIKEDNTTMTRCGTP-CWTAPEVI---------RGEKYCE 1562

Query: 434  KSDVYSFGMVCFEVLTGKVPFEDSHLQGDKMSRNIRAGERPLFPHSMPKYVTNLTKRCWH 493
            K+DV+SFG+V +EVLTGK PF + +    K+S +I  G RP+ P   P     L K+CWH
Sbjct: 1563 KADVFSFGVVMWEVLTGKEPFAECNFM--KVSLDILEGGRPIIPSDCPHEFAKLIKKCWH 1620

Query: 494  TDPNQRPSFPSIC-RILRYTKRF 508
               ++RP+   +  +++  T++F
Sbjct: 1623 AKAHKRPTMTEVVQQLMLITEQF 1620

BLAST of Bhi01M000305 vs. Swiss-Prot
Match: sp|Q24592|JAK_DROME (Tyrosine-protein kinase hopscotch OS=Drosophila melanogaster OX=7227 GN=hop PE=1 SV=2)

HSP 1 Score: 114.0 bits (284), Expect = 6.5e-24
Identity = 82/261 (31.42%), Postives = 125/261 (47.89%), Query Frame = 0

Query: 274  EISMLLSLSHPNITRFLCGFTDEEKKECFLIMELMSRDLSSYVKEICGPRKRMPFPLPVA 333
            EI ++ +LSHPNI +F       EK  C ++  L S     Y++    P    P  +  A
Sbjct: 940  EIGIMRTLSHPNIVKFKYW---AEKSHCIIMEYLQSGSFDIYLR-FTAPNLNNPRLVSFA 999

Query: 334  LDLMLQIARGMEYLHSKKIYHGDLNPCNILVKPRAYSTDGYVHAQVSGFGLPVVKFKNS- 393
            LD    IA GM+YL    + H DL   NILV    ++ DG    ++S FGL   +F NS 
Sbjct: 1000 LD----IANGMKYLSDMGLIHRDLAARNILVD---HNGDGDC-VKISDFGL--AQFANSD 1059

Query: 394  ----SNQNESLPFIWYAPEVLEEQDQSGSAESSKYTEKSDVYSFGMVCFEVLT-----GK 453
                +     +P  WY+PE +          + +++  SDV+S+G+  FE+ +       
Sbjct: 1060 GYYYAKSKRDIPIRWYSPEAI---------STCRFSSYSDVWSYGVTLFEMFSRGEEPNL 1119

Query: 454  VPFEDSHLQGDKMSRNIRAGERPLFPHSMPKYVTNLTKRCWHTDPNQRPSFPSICRILRY 513
            VP + S  Q D ++R +++GER   P S P ++ +L + CWH  P  RPSF +I  I+  
Sbjct: 1120 VPIQTS--QEDFLNR-LQSGERLNRPASCPDFIYDLMQLCWHATPRSRPSFATIVDIITR 1174

Query: 514  TKRFVAMNPDYNSQTDPAMPT 525
                   +P    Q+ P  PT
Sbjct: 1180 EVATKVTHPTDGHQSPPNQPT 1174

BLAST of Bhi01M000305 vs. TrEMBL
Match: tr|A0A1S3AVV1|A0A1S3AVV1_CUCME (mitogen-activated protein kinase kinase kinase 7-like OS=Cucumis melo OX=3656 GN=LOC103483226 PE=4 SV=1)

HSP 1 Score: 1308.5 bits (3385), Expect = 0.0e+00
Identity = 638/679 (93.96%), Postives = 657/679 (96.76%), Query Frame = 0

Query: 1   MEQFRQLGEALGSVKALMLFKDSVHLNQRQCCLLLDFLSFAYDSVAEEMKQNLRFEEKHT 60
           MEQFRQLGEALGSVKALMLFKDSVH+NQRQCCLLLD LSFAYDSVAEEMKQNLRFEEKHT
Sbjct: 1   MEQFRQLGEALGSVKALMLFKDSVHINQRQCCLLLDVLSFAYDSVAEEMKQNLRFEEKHT 60

Query: 61  RWKVLDQPLRDLNRVFKEAEWYIRQCLETKDWWAKAIMLYQNTDCIELHIHNLLYSITVV 120
           RWKVLDQPLRDLNRVFKEAEWYIRQCLETKDWWAK I LYQNTDCIE HIHNLLYSITVV
Sbjct: 61  RWKVLDQPLRDLNRVFKEAEWYIRQCLETKDWWAKVITLYQNTDCIEFHIHNLLYSITVV 120

Query: 121 VEAIETAGENSGSDHDDMLKKRLINSIKYRREHKDLKIFKWKFGKQYLITQDFCNRMDAV 180
           VEAIETAGENSGSDHD++LKKRLINSIKYRREHKDLKIFKWKFGKQYLIT+DFCNR+DAV
Sbjct: 121 VEAIETAGENSGSDHDELLKKRLINSIKYRREHKDLKIFKWKFGKQYLITRDFCNRIDAV 180

Query: 181 WNEDRWVLLNKIREKKFTASSKYEQRLTDLLLKNLDGPESLNGKLLPSSMLVGSRDYQVR 240
           WNEDRW LLNKIREKK TASSKYEQRLTD L+KN++G ESLNGKLLPSSMLVGS+DYQVR
Sbjct: 181 WNEDRWFLLNKIREKKSTASSKYEQRLTDHLMKNINGSESLNGKLLPSSMLVGSKDYQVR 240

Query: 241 RRLGGGSQYKEILWLGESFAMRHFFGEIESLIPEISMLLSLSHPNITRFLCGFTDEEKKE 300
           RRLGGGSQYKEILWLGESFAMRHFFGEIESLIPEIS+LLSLSHPNITRFLCGFTDEEKKE
Sbjct: 241 RRLGGGSQYKEILWLGESFAMRHFFGEIESLIPEISILLSLSHPNITRFLCGFTDEEKKE 300

Query: 301 CFLIMELMSRDLSSYVKEICGPRKRMPFPLPVALDLMLQIARGMEYLHSKKIYHGDLNPC 360
           CFLIMELMSRDLSSYVKEICGPRKR+PFPLPVALDLMLQIARGMEYLHSKK+YHGDLNPC
Sbjct: 301 CFLIMELMSRDLSSYVKEICGPRKRIPFPLPVALDLMLQIARGMEYLHSKKVYHGDLNPC 360

Query: 361 NILVKPRAYSTDGYVHAQVSGFGLPVVKFKNSSNQNESLPFIWYAPEVLEEQDQSGSAES 420
           NILVKPRAYSTDGYVH QVSGFGLP VKFKNSSNQNESLPFIWYAPEVLEEQDQSGSAES
Sbjct: 361 NILVKPRAYSTDGYVHGQVSGFGLPAVKFKNSSNQNESLPFIWYAPEVLEEQDQSGSAES 420

Query: 421 SKYTEKSDVYSFGMVCFEVLTGKVPFEDSHLQGDKMSRNIRAGERPLFPHSMPKYVTNLT 480
           SKYTEKSDVYSFGMVCFEVLTGKVPFEDSHLQGDKMSRNIRAGERPLFPHSMPKYVTNLT
Sbjct: 421 SKYTEKSDVYSFGMVCFEVLTGKVPFEDSHLQGDKMSRNIRAGERPLFPHSMPKYVTNLT 480

Query: 481 KRCWHTDPNQRPSFPSICRILRYTKRFVAMNPDYNSQTDPAMPTVDYCDIESGLLRRLPS 540
           KRCWHTDPNQRPSF SICRILRYTKRFVAMNPDYNSQTDPAMPTVDYCDIESGLLRRLP 
Sbjct: 481 KRCWHTDPNQRPSFTSICRILRYTKRFVAMNPDYNSQTDPAMPTVDYCDIESGLLRRLPP 540

Query: 541 CGISEASSAITDIPFQMFAYRIVEKEKAGANIKDTSESGSDASACGDETTSSVDDPFPTL 600
           CGIS+A+S ITDIPFQMFAYRIVEKE+AGA  KDTSESGSDASACGDET SS+DDPFPT 
Sbjct: 541 CGISDAASPITDIPFQMFAYRIVEKERAGATFKDTSESGSDASACGDETGSSIDDPFPTS 600

Query: 601 VEKKLPVSREGTTRRLSLTKNNSDIKLNKLPGTPKGRFSRPPQISPRGRSMRMNSESQLM 660
           VEKKLP  REG +RRLSLTKNNSDIKLNKLPGTPKGRFSRPPQISPRGRS+RMNSESQLM
Sbjct: 601 VEKKLPAPREG-SRRLSLTKNNSDIKLNKLPGTPKGRFSRPPQISPRGRSLRMNSESQLM 660

Query: 661 AMSPKVRRLSGHASDSELP 680
            +SPKVRR SGHASDSELP
Sbjct: 661 TISPKVRRSSGHASDSELP 678

BLAST of Bhi01M000305 vs. TrEMBL
Match: tr|A0A0A0L614|A0A0A0L614_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G120430 PE=4 SV=1)

HSP 1 Score: 1292.7 bits (3344), Expect = 0.0e+00
Identity = 628/679 (92.49%), Postives = 652/679 (96.02%), Query Frame = 0

Query: 1   MEQFRQLGEALGSVKALMLFKDSVHLNQRQCCLLLDFLSFAYDSVAEEMKQNLRFEEKHT 60
           MEQFRQLGEALGSVKALMLFKDSVH+NQRQCCLLLD LSFAYDSVAEEMKQNLRFEEKHT
Sbjct: 1   MEQFRQLGEALGSVKALMLFKDSVHINQRQCCLLLDVLSFAYDSVAEEMKQNLRFEEKHT 60

Query: 61  RWKVLDQPLRDLNRVFKEAEWYIRQCLETKDWWAKAIMLYQNTDCIELHIHNLLYSITVV 120
           RWKVLDQPLRDLNRVFKEAEWYIRQCLETKDWWAK IMLYQNTDCIE HIHNLLYSITVV
Sbjct: 61  RWKVLDQPLRDLNRVFKEAEWYIRQCLETKDWWAKVIMLYQNTDCIEFHIHNLLYSITVV 120

Query: 121 VEAIETAGENSGSDHDDMLKKRLINSIKYRREHKDLKIFKWKFGKQYLITQDFCNRMDAV 180
           VEAIE AGE+SGSDHD++LKK+LINSIKYRREHKDLKIFKWKFGKQYL+TQDFCNR++AV
Sbjct: 121 VEAIEMAGESSGSDHDELLKKKLINSIKYRREHKDLKIFKWKFGKQYLVTQDFCNRIEAV 180

Query: 181 WNEDRWVLLNKIREKKFTASSKYEQRLTDLLLKNLDGPESLNGKLLPSSMLVGSRDYQVR 240
           WNEDRW LLNKIREKK  ASSKYEQRLTD LLKN++G ES NGKLLPS MLVGS+DYQVR
Sbjct: 181 WNEDRWFLLNKIREKKLMASSKYEQRLTDHLLKNINGSESFNGKLLPSLMLVGSKDYQVR 240

Query: 241 RRLGGGSQYKEILWLGESFAMRHFFGEIESLIPEISMLLSLSHPNITRFLCGFTDEEKKE 300
           RRLG GSQYKEILWLGESFAMRHFFGEIESLIPEISMLLSLSHPNITRFLCGFTDEEKKE
Sbjct: 241 RRLGVGSQYKEILWLGESFAMRHFFGEIESLIPEISMLLSLSHPNITRFLCGFTDEEKKE 300

Query: 301 CFLIMELMSRDLSSYVKEICGPRKRMPFPLPVALDLMLQIARGMEYLHSKKIYHGDLNPC 360
           CFLIMELMSRDLS YVKEICGPRKR+PF LPVALDLMLQIARGMEYLHSKK+YHGDLNPC
Sbjct: 301 CFLIMELMSRDLSGYVKEICGPRKRIPFTLPVALDLMLQIARGMEYLHSKKVYHGDLNPC 360

Query: 361 NILVKPRAYSTDGYVHAQVSGFGLPVVKFKNSSNQNESLPFIWYAPEVLEEQDQSGSAES 420
           NILVKPRAYSTDGYVH QVSGFGLP VKFKNSSNQNESLPFIWYAPEVLEEQDQSGSAES
Sbjct: 361 NILVKPRAYSTDGYVHGQVSGFGLPAVKFKNSSNQNESLPFIWYAPEVLEEQDQSGSAES 420

Query: 421 SKYTEKSDVYSFGMVCFEVLTGKVPFEDSHLQGDKMSRNIRAGERPLFPHSMPKYVTNLT 480
            KYTEKSDVYSFGMVCFEVLTGKVPFEDSHLQGDKMSRNIRAGERPLFPHSMPKYVTNLT
Sbjct: 421 CKYTEKSDVYSFGMVCFEVLTGKVPFEDSHLQGDKMSRNIRAGERPLFPHSMPKYVTNLT 480

Query: 481 KRCWHTDPNQRPSFPSICRILRYTKRFVAMNPDYNSQTDPAMPTVDYCDIESGLLRRLPS 540
           KRCW TDPNQRPSF SICRILRYTKRFVAMNPDYNSQTDPAMPTVDYCDIESGLLRRLPS
Sbjct: 481 KRCWQTDPNQRPSFTSICRILRYTKRFVAMNPDYNSQTDPAMPTVDYCDIESGLLRRLPS 540

Query: 541 CGISEASSAITDIPFQMFAYRIVEKEKAGANIKDTSESGSDASACGDETTSSVDDPFPTL 600
           CGIS+A+S ITDIPFQMFAYR+VEKE+AGA  KDTSESGSDASACGDET SS+DDPFPT 
Sbjct: 541 CGISDAASPITDIPFQMFAYRVVEKERAGATFKDTSESGSDASACGDETASSIDDPFPTP 600

Query: 601 VEKKLPVSREGTTRRLSLTKNNSDIKLNKLPGTPKGRFSRPPQISPRGRSMRMNSESQLM 660
           VE+KLP  REG +RRLSLTKNNSD++LNKLPGTPKGRFSRPPQISPRGRSMRMNSESQLM
Sbjct: 601 VERKLPAPREG-SRRLSLTKNNSDVRLNKLPGTPKGRFSRPPQISPRGRSMRMNSESQLM 660

Query: 661 AMSPKVRRLSGHASDSELP 680
           A+SPK+RRLSGHASDSELP
Sbjct: 661 AISPKIRRLSGHASDSELP 678

BLAST of Bhi01M000305 vs. TrEMBL
Match: tr|A0A251QHS9|A0A251QHS9_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_2G183700 PE=4 SV=1)

HSP 1 Score: 988.8 bits (2555), Expect = 6.1e-285
Identity = 487/687 (70.89%), Postives = 572/687 (83.26%), Query Frame = 0

Query: 1   MEQFRQLGEALGSVKALMLFKDSVHLNQRQCCLLLDFLSFAYDSVAEEMKQNLRFEEKHT 60
           MEQFR++GE LGS+KALM+F+D++ +NQRQC LLLD  S AY+S+AEEMK NLRFEEKHT
Sbjct: 1   MEQFRKIGEDLGSLKALMVFQDNIQINQRQCFLLLDIFSSAYESIAEEMKYNLRFEEKHT 60

Query: 61  RWKVLDQPLRDLNRVFKEAEWYIRQCLETKDWWAKAIMLYQNTDCIELHIHNLLYSITVV 120
           +W+VL+QPLR+L+R+FKE E YIR CLETKDWWAKAI LYQN+DCIE HIHNLL  + +V
Sbjct: 61  KWRVLEQPLRELHRIFKEGEAYIRHCLETKDWWAKAITLYQNSDCIEFHIHNLLSCMPIV 120

Query: 121 VEAIETAGENSGSDHDDMLKKRLINSIKYRREHKDLKIFKWKFGKQYLITQDFCNRMDAV 180
           +EAI+ AGE SG   D++ +K+ + + KY+ +++D K+FKW+FGKQYLITQDFCNR D  
Sbjct: 121 IEAIDIAGEISGWGQDEIQRKKTVFADKYKDDYRDWKLFKWRFGKQYLITQDFCNRFDTT 180

Query: 181 WNEDRWVLLNKIREKKFTASSKYEQRLTDLLLKNLDGPES--LNGKLLPSSMLVGSRDYQ 240
           W EDRW LL+KIREKK + S+KY +RL DLL K+LDG ES  LNGKLLPSS+LVGS+DYQ
Sbjct: 181 WKEDRWTLLHKIREKKLSGSTKYGKRLIDLLFKSLDGSESQPLNGKLLPSSILVGSKDYQ 240

Query: 241 VRRRLGGGSQYKEILWLGESFAMRHFFGEIESLIPEISMLLSLSHPNITRFLCGFTDEEK 300
           VRRRLGGGSQYKEILWLGESFA RHFFG+IE L+PEIS LLSLSHPNI  FLCGFTDEEK
Sbjct: 241 VRRRLGGGSQYKEILWLGESFASRHFFGDIEPLLPEISSLLSLSHPNIVHFLCGFTDEEK 300

Query: 301 KECFLIMELMSRDLSSYVKEICGPRKRMPFPLPVALDLMLQIARGMEYLHSKKIYHGDLN 360
           KECFLIMELMSRDL S++KEICGPRKR+PF LPVA+DLMLQIARGMEYLHSKKIYHG+LN
Sbjct: 301 KECFLIMELMSRDLCSHIKEICGPRKRLPFSLPVAVDLMLQIARGMEYLHSKKIYHGELN 360

Query: 361 PCNILVKPRAYSTDGYVHAQVSGFGLPVVKF---KNSSNQNESLPFIWYAPEVLEEQDQS 420
           PCNILVK R  STDGY+ A+VSGFGL   K    KN SNQN SLPFIWYAPEVLEE +Q+
Sbjct: 361 PCNILVKARGISTDGYLQAKVSGFGLTSAKSPSQKNLSNQNGSLPFIWYAPEVLEEHEQT 420

Query: 421 GSAESSKYTEKSDVYSFGMVCFEVLTGKVPFEDSHLQGDKMSRNIRAGERPLFPHSMPKY 480
            S+E  KYTEKSDVYSFGMVCFE+LTGKVPFEDSHLQG+KMSRNIRAGERPLFP    +Y
Sbjct: 421 KSSE-KKYTEKSDVYSFGMVCFELLTGKVPFEDSHLQGEKMSRNIRAGERPLFPFYSLRY 480

Query: 481 VTNLTKRCWHTDPNQRPSFPSICRILRYTKRFVAMNPDYNSQTDPAMPTVDYCDIESGLL 540
           VTNLTK+CWH+DPN RPSF SICRILRY KRF+AMNPDYNSQ DP +P VDYC+IESG+L
Sbjct: 481 VTNLTKKCWHSDPNLRPSFSSICRILRYIKRFLAMNPDYNSQLDPPVPMVDYCEIESGIL 540

Query: 541 RRLPSCGISEASSAITDIPFQMFAYRIVEKEKAGANIKDTSESGSD-ASACGDETTSSVD 600
           R++PS    E    ++ IPFQMF YRI E+E+    +KDTSESGSD AS CGDE  ++ D
Sbjct: 541 RKIPSWKSCE-QPPVSQIPFQMFVYRIAERER--TTLKDTSESGSDGASICGDEMVTTPD 600

Query: 601 DPFPTLVEKKLPVSREGTTRRLSLTKNNSDIKLNKLPGTPKGRFSRPPQISPRGR--SMR 660
           +PFP   E+K   S +   ++L   K +SD+K N+LPGTP+GR  RPPQ+SP GR  S+R
Sbjct: 601 EPFPPAPERKCLTSPDSMKKKLPFLKKSSDVKANRLPGTPRGRSVRPPQMSPCGRSISLR 660

Query: 661 MNSESQLMAMSPKVRRL-SGHASDSEL 679
           M+SESQLMAM+P++RR  SGHASDSEL
Sbjct: 661 MSSESQLMAMTPRIRRTSSGHASDSEL 683

BLAST of Bhi01M000305 vs. TrEMBL
Match: tr|M5X3P7|M5X3P7_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_ppa021542mg PE=4 SV=1)

HSP 1 Score: 982.6 bits (2539), Expect = 4.3e-283
Identity = 487/692 (70.38%), Postives = 572/692 (82.66%), Query Frame = 0

Query: 1   MEQFRQLGEALGSVKALMLFKDSVHLNQRQCCLLLDFLSFAYDSVAEEMKQNLRFEEKHT 60
           MEQFR++GE LGS+KALM+F+D++ +NQRQC LLLD  S AY+S+AEEMK NLRFEEKHT
Sbjct: 1   MEQFRKIGEDLGSLKALMVFQDNIQINQRQCFLLLDIFSSAYESIAEEMKYNLRFEEKHT 60

Query: 61  RWKVLDQPLRDLNRVFKEAEWYIRQCLETKDWWAKAIMLYQNTDCIELHIHNLLYSITVV 120
           +W+VL+QPLR+L+R+FKE E YIR CLETKDWWAKAI LYQN+DCIE HIHNLL  + +V
Sbjct: 61  KWRVLEQPLRELHRIFKEGEAYIRHCLETKDWWAKAITLYQNSDCIEFHIHNLLSCMPIV 120

Query: 121 VEAIETAGENSGSDHDDMLKKRLINSIKYRREHKDLKIFKWKFGKQYLITQDFCNRMDAV 180
           +EAI+ AGE SG   D++ +K+ + + KY+ +++D K+FKW+FGKQYLITQDFCNR D  
Sbjct: 121 IEAIDIAGEISGWGQDEIQRKKTVFADKYKDDYRDWKLFKWRFGKQYLITQDFCNRFDTT 180

Query: 181 WNEDRWVLLNKIREKKFTASSKYEQRLTDLLLKNLDGPES--LNGKLLPSSMLVGSRDYQ 240
           W EDRW LL+KIREKK + S+KY +RL DLL K+LDG ES  LNGKLLPSS+LVGS+DYQ
Sbjct: 181 WKEDRWTLLHKIREKKLSGSTKYGKRLIDLLFKSLDGSESQPLNGKLLPSSILVGSKDYQ 240

Query: 241 VRRRLGGGSQYKEILWLGESFAMRHFFGEIESLIPEISMLLSLSHPNITRFLCGFTDEEK 300
           VRRRLGGGSQYKEILWLGESFA RHFFG+IE L+PEIS LLSLSHPNI  FLCGFTDEEK
Sbjct: 241 VRRRLGGGSQYKEILWLGESFASRHFFGDIEPLLPEISSLLSLSHPNIVHFLCGFTDEEK 300

Query: 301 KECFLIMELMSRDLSSYVKEICGPRKRMPFPLPVALDLMLQIARGMEYLHSKKIYHGDLN 360
           KECFLIMELMSRDL S++KEICGPRKR+PF LPVA+DLMLQIARGMEYLHSKKIYHG+LN
Sbjct: 301 KECFLIMELMSRDLCSHIKEICGPRKRLPFSLPVAVDLMLQIARGMEYLHSKKIYHGELN 360

Query: 361 PCNILVKPRAYSTDGYVHAQVSGFGLPVVKF---KNSSNQNESLPFIWYAPEVLEEQDQS 420
           PCNILVK R  STDGY+ A+VSGFGL   K    KN SNQN SLPFIWYAPEVLEE +Q+
Sbjct: 361 PCNILVKARGISTDGYLQAKVSGFGLTSAKSPSQKNLSNQNGSLPFIWYAPEVLEEHEQT 420

Query: 421 GSAESSKYTEKSDVYSFGMVCFEVLTGKVPFEDSHLQGDKMSRNIRAGERPLFPHSMPKY 480
            S+E  KYTEKSDVYSFGMVCFE+LTGKVPFEDSHLQG+KMSRNIRAGERPLFP    +Y
Sbjct: 421 KSSE-KKYTEKSDVYSFGMVCFELLTGKVPFEDSHLQGEKMSRNIRAGERPLFPFYSLRY 480

Query: 481 VTNLTKRCWHTDPNQRPSFPSICRILRYTKRFVAMNPDYNSQTDPAMPTVDYCDIESGLL 540
           VTNLTK+CWH+DPN RPSF SICRILRY KRF+AMNPDYNSQ DP +P VDYC+IESG+L
Sbjct: 481 VTNLTKKCWHSDPNLRPSFSSICRILRYIKRFLAMNPDYNSQLDPPVPMVDYCEIESGIL 540

Query: 541 RRLPSCGISEASSAITDIPFQMFAYRIVEKEKAGANIKDTSESGSD-ASACGDETTSSVD 600
           R++PS    E    ++ IPFQMF YRI E+E+    +KDTSESGSD AS CGDE  ++ D
Sbjct: 541 RKIPSWKSCE-QPPVSQIPFQMFVYRIAERER--TTLKDTSESGSDGASICGDEMVTTPD 600

Query: 601 DPFPTLVEKKLPVSREGTTRRLSLTKNNSDIKLNKLP-----GTPKGRFSRPPQISPRGR 660
           +PFP   E+K   S +   ++L   K +SD+K N+LP     GTP+GR  RPPQ+SP GR
Sbjct: 601 EPFPPAPERKCLTSPDSMKKKLPFLKKSSDVKANRLPGFGYTGTPRGRSVRPPQMSPCGR 660

Query: 661 --SMRMNSESQLMAMSPKVRRL-SGHASDSEL 679
             S+RM+SESQLMAM+P++RR  SGHASDSEL
Sbjct: 661 SISLRMSSESQLMAMTPRIRRTSSGHASDSEL 688

BLAST of Bhi01M000305 vs. TrEMBL
Match: tr|A0A2C9WNU9|A0A2C9WNU9_MANES (Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_01G248800 PE=4 SV=1)

HSP 1 Score: 963.8 bits (2490), Expect = 2.1e-277
Identity = 473/688 (68.75%), Postives = 569/688 (82.70%), Query Frame = 0

Query: 1   MEQFRQLGEALGSVKALMLFKDSVHLNQRQCCLLLDFLSFAYDSVAEEMKQNLRFEEKHT 60
           MEQFRQ+GE LGS+KA+M+F+D++ +NQRQCCLLLD  + AYDS+AEEMKQNL+FEEKHT
Sbjct: 1   MEQFRQIGEVLGSLKAVMVFRDNIEINQRQCCLLLDMFNSAYDSIAEEMKQNLKFEEKHT 60

Query: 61  RWKVLDQPLRDLNRVFKEAEWYIRQCLETKDWWAKAIMLYQNTDCIELHIHNLLYSITVV 120
           +W++L+QPLR++ R+FKE E YI+QCLETKDW AKAI+L QNTDC++ +IHNLL  IT+V
Sbjct: 61  KWRLLEQPLREIYRIFKEGEGYIKQCLETKDWCAKAIVLSQNTDCVQFYIHNLLTYITIV 120

Query: 121 VEAIETAGENSGSDHDDMLKKRLINSIKYRREHKDLKIFKWKFGKQYLITQDFCNRMDAV 180
           +EAIET GE SG D D++ KKRL+ S KY+++ +D ++F+W+FGKQ+LITQ+ C R  +V
Sbjct: 121 IEAIETVGEFSGWDQDEVQKKRLLYSNKYQKQWRDPQLFQWRFGKQHLITQELCERFASV 180

Query: 181 WNEDRWVLLNKIREKKFTASSKYEQRLTDLLLKNLDGPESLNGKLLPSSMLVGSRDYQVR 240
           W EDRW+LLNKIREKK + SSKYE++L +LLLKNLDG E  N KLLPSS+LVGS+DYQVR
Sbjct: 181 WKEDRWILLNKIREKKISCSSKYERQLAELLLKNLDGSEHQNKKLLPSSILVGSKDYQVR 240

Query: 241 RRLGGGSQYKEILWLGESFAMRHFFGEIESLIPEISMLLSLSHPNITRFLCGFTDEEKKE 300
           RRLG GSQYKEILWLGES A+RHFFGEIE L+PEIS LLSLSH NI+ FLCGF DEEK E
Sbjct: 241 RRLGSGSQYKEILWLGESLAIRHFFGEIEHLVPEISSLLSLSHTNISHFLCGFADEEKNE 300

Query: 301 CFLIMELMSRDLSSYVKEICGPRKRMPFPLPVALDLMLQIARGMEYLHSKKIYHGDLNPC 360
           CFL+MELM RDLSSY++EICGPRKR+PF LPVALDLMLQIARGMEYLHSKKIYHG L P 
Sbjct: 301 CFLVMELMHRDLSSYIREICGPRKRIPFSLPVALDLMLQIARGMEYLHSKKIYHGKLKPS 360

Query: 361 NILVKPRAYSTDGYVHAQVSGFGL-PVVKF--KNSSNQNESLPFIWYAPEVLEEQDQSGS 420
           NILVKPR  S++GY+HA+VSGFGL  V KF  KNSSNQNE+LPFIWYAPEVLE Q+Q+GS
Sbjct: 361 NILVKPRNVSSEGYLHAKVSGFGLSSVQKFSPKNSSNQNETLPFIWYAPEVLEAQEQAGS 420

Query: 421 AESSKYTEKSDVYSFGMVCFEVLTGKVPFEDSHLQGDKMSRNIRAGERPLFPHSMPKYVT 480
             +SKYTEKSDVYSFGMVCFE++TGKVPFEDSHLQGDKMSRNIRAGERPLFP + PK+VT
Sbjct: 421 TRNSKYTEKSDVYSFGMVCFELVTGKVPFEDSHLQGDKMSRNIRAGERPLFPLNSPKFVT 480

Query: 481 NLTKRCWHTDPNQRPSFPSICRILRYTKRFVAMNPDYNSQTDPAMPTVDYCDIESGLLRR 540
           NLTKRCW  DPNQRPSF SICRILRY KRF+AMNPDYN + DP MP +DY D+ES L++ 
Sbjct: 481 NLTKRCWQADPNQRPSFSSICRILRYAKRFLAMNPDYNREPDPLMPAMDYGDMESNLVKE 540

Query: 541 LPSCGISEASSAITDIPFQMFAYRIVEKEKAGANIKDTSESGSD-ASACGDETTSSVDDP 600
            PS   +  SSAI  IPFQ+F Y++ EKEK  A  K+ SESGSD AS  GDE  +S+DDP
Sbjct: 541 FPSWNATN-SSAIAQIPFQIFVYKVYEKEKTRATQKEPSESGSDKASVSGDENINSMDDP 600

Query: 601 FPTL-----VEKKLPVSREGTTRRLSLTKNNSDIKLNKLPGTPKGRFSRPPQISPRGRSM 660
           FP+       E+K   S + T R+   ++  +D+K+++ PGT KG   +PPQ++ RGR +
Sbjct: 601 FPSSTLPSPTERKFLTSPDHTNRKPRPSRKPTDLKISRQPGTAKGG-CKPPQLTSRGRGL 660

Query: 661 RMNSESQLMAMSPKVRR-LSGHASDSEL 679
           RMNSESQL+  SP++RR  SGHASDSEL
Sbjct: 661 RMNSESQLIMTSPRLRRSSSGHASDSEL 686

BLAST of Bhi01M000305 vs. NCBI nr
Match: XP_008437968.1 (PREDICTED: mitogen-activated protein kinase kinase kinase 7-like [Cucumis melo])

HSP 1 Score: 1308.5 bits (3385), Expect = 0.0e+00
Identity = 638/679 (93.96%), Postives = 657/679 (96.76%), Query Frame = 0

Query: 1   MEQFRQLGEALGSVKALMLFKDSVHLNQRQCCLLLDFLSFAYDSVAEEMKQNLRFEEKHT 60
           MEQFRQLGEALGSVKALMLFKDSVH+NQRQCCLLLD LSFAYDSVAEEMKQNLRFEEKHT
Sbjct: 1   MEQFRQLGEALGSVKALMLFKDSVHINQRQCCLLLDVLSFAYDSVAEEMKQNLRFEEKHT 60

Query: 61  RWKVLDQPLRDLNRVFKEAEWYIRQCLETKDWWAKAIMLYQNTDCIELHIHNLLYSITVV 120
           RWKVLDQPLRDLNRVFKEAEWYIRQCLETKDWWAK I LYQNTDCIE HIHNLLYSITVV
Sbjct: 61  RWKVLDQPLRDLNRVFKEAEWYIRQCLETKDWWAKVITLYQNTDCIEFHIHNLLYSITVV 120

Query: 121 VEAIETAGENSGSDHDDMLKKRLINSIKYRREHKDLKIFKWKFGKQYLITQDFCNRMDAV 180
           VEAIETAGENSGSDHD++LKKRLINSIKYRREHKDLKIFKWKFGKQYLIT+DFCNR+DAV
Sbjct: 121 VEAIETAGENSGSDHDELLKKRLINSIKYRREHKDLKIFKWKFGKQYLITRDFCNRIDAV 180

Query: 181 WNEDRWVLLNKIREKKFTASSKYEQRLTDLLLKNLDGPESLNGKLLPSSMLVGSRDYQVR 240
           WNEDRW LLNKIREKK TASSKYEQRLTD L+KN++G ESLNGKLLPSSMLVGS+DYQVR
Sbjct: 181 WNEDRWFLLNKIREKKSTASSKYEQRLTDHLMKNINGSESLNGKLLPSSMLVGSKDYQVR 240

Query: 241 RRLGGGSQYKEILWLGESFAMRHFFGEIESLIPEISMLLSLSHPNITRFLCGFTDEEKKE 300
           RRLGGGSQYKEILWLGESFAMRHFFGEIESLIPEIS+LLSLSHPNITRFLCGFTDEEKKE
Sbjct: 241 RRLGGGSQYKEILWLGESFAMRHFFGEIESLIPEISILLSLSHPNITRFLCGFTDEEKKE 300

Query: 301 CFLIMELMSRDLSSYVKEICGPRKRMPFPLPVALDLMLQIARGMEYLHSKKIYHGDLNPC 360
           CFLIMELMSRDLSSYVKEICGPRKR+PFPLPVALDLMLQIARGMEYLHSKK+YHGDLNPC
Sbjct: 301 CFLIMELMSRDLSSYVKEICGPRKRIPFPLPVALDLMLQIARGMEYLHSKKVYHGDLNPC 360

Query: 361 NILVKPRAYSTDGYVHAQVSGFGLPVVKFKNSSNQNESLPFIWYAPEVLEEQDQSGSAES 420
           NILVKPRAYSTDGYVH QVSGFGLP VKFKNSSNQNESLPFIWYAPEVLEEQDQSGSAES
Sbjct: 361 NILVKPRAYSTDGYVHGQVSGFGLPAVKFKNSSNQNESLPFIWYAPEVLEEQDQSGSAES 420

Query: 421 SKYTEKSDVYSFGMVCFEVLTGKVPFEDSHLQGDKMSRNIRAGERPLFPHSMPKYVTNLT 480
           SKYTEKSDVYSFGMVCFEVLTGKVPFEDSHLQGDKMSRNIRAGERPLFPHSMPKYVTNLT
Sbjct: 421 SKYTEKSDVYSFGMVCFEVLTGKVPFEDSHLQGDKMSRNIRAGERPLFPHSMPKYVTNLT 480

Query: 481 KRCWHTDPNQRPSFPSICRILRYTKRFVAMNPDYNSQTDPAMPTVDYCDIESGLLRRLPS 540
           KRCWHTDPNQRPSF SICRILRYTKRFVAMNPDYNSQTDPAMPTVDYCDIESGLLRRLP 
Sbjct: 481 KRCWHTDPNQRPSFTSICRILRYTKRFVAMNPDYNSQTDPAMPTVDYCDIESGLLRRLPP 540

Query: 541 CGISEASSAITDIPFQMFAYRIVEKEKAGANIKDTSESGSDASACGDETTSSVDDPFPTL 600
           CGIS+A+S ITDIPFQMFAYRIVEKE+AGA  KDTSESGSDASACGDET SS+DDPFPT 
Sbjct: 541 CGISDAASPITDIPFQMFAYRIVEKERAGATFKDTSESGSDASACGDETGSSIDDPFPTS 600

Query: 601 VEKKLPVSREGTTRRLSLTKNNSDIKLNKLPGTPKGRFSRPPQISPRGRSMRMNSESQLM 660
           VEKKLP  REG +RRLSLTKNNSDIKLNKLPGTPKGRFSRPPQISPRGRS+RMNSESQLM
Sbjct: 601 VEKKLPAPREG-SRRLSLTKNNSDIKLNKLPGTPKGRFSRPPQISPRGRSLRMNSESQLM 660

Query: 661 AMSPKVRRLSGHASDSELP 680
            +SPKVRR SGHASDSELP
Sbjct: 661 TISPKVRRSSGHASDSELP 678

BLAST of Bhi01M000305 vs. NCBI nr
Match: XP_004134312.1 (PREDICTED: light-sensor Protein kinase-like [Cucumis sativus] >XP_011650725.1 PREDICTED: light-sensor Protein kinase-like [Cucumis sativus] >KGN56464.1 hypothetical protein Csa_3G120430 [Cucumis sativus])

HSP 1 Score: 1292.7 bits (3344), Expect = 0.0e+00
Identity = 628/679 (92.49%), Postives = 652/679 (96.02%), Query Frame = 0

Query: 1   MEQFRQLGEALGSVKALMLFKDSVHLNQRQCCLLLDFLSFAYDSVAEEMKQNLRFEEKHT 60
           MEQFRQLGEALGSVKALMLFKDSVH+NQRQCCLLLD LSFAYDSVAEEMKQNLRFEEKHT
Sbjct: 1   MEQFRQLGEALGSVKALMLFKDSVHINQRQCCLLLDVLSFAYDSVAEEMKQNLRFEEKHT 60

Query: 61  RWKVLDQPLRDLNRVFKEAEWYIRQCLETKDWWAKAIMLYQNTDCIELHIHNLLYSITVV 120
           RWKVLDQPLRDLNRVFKEAEWYIRQCLETKDWWAK IMLYQNTDCIE HIHNLLYSITVV
Sbjct: 61  RWKVLDQPLRDLNRVFKEAEWYIRQCLETKDWWAKVIMLYQNTDCIEFHIHNLLYSITVV 120

Query: 121 VEAIETAGENSGSDHDDMLKKRLINSIKYRREHKDLKIFKWKFGKQYLITQDFCNRMDAV 180
           VEAIE AGE+SGSDHD++LKK+LINSIKYRREHKDLKIFKWKFGKQYL+TQDFCNR++AV
Sbjct: 121 VEAIEMAGESSGSDHDELLKKKLINSIKYRREHKDLKIFKWKFGKQYLVTQDFCNRIEAV 180

Query: 181 WNEDRWVLLNKIREKKFTASSKYEQRLTDLLLKNLDGPESLNGKLLPSSMLVGSRDYQVR 240
           WNEDRW LLNKIREKK  ASSKYEQRLTD LLKN++G ES NGKLLPS MLVGS+DYQVR
Sbjct: 181 WNEDRWFLLNKIREKKLMASSKYEQRLTDHLLKNINGSESFNGKLLPSLMLVGSKDYQVR 240

Query: 241 RRLGGGSQYKEILWLGESFAMRHFFGEIESLIPEISMLLSLSHPNITRFLCGFTDEEKKE 300
           RRLG GSQYKEILWLGESFAMRHFFGEIESLIPEISMLLSLSHPNITRFLCGFTDEEKKE
Sbjct: 241 RRLGVGSQYKEILWLGESFAMRHFFGEIESLIPEISMLLSLSHPNITRFLCGFTDEEKKE 300

Query: 301 CFLIMELMSRDLSSYVKEICGPRKRMPFPLPVALDLMLQIARGMEYLHSKKIYHGDLNPC 360
           CFLIMELMSRDLS YVKEICGPRKR+PF LPVALDLMLQIARGMEYLHSKK+YHGDLNPC
Sbjct: 301 CFLIMELMSRDLSGYVKEICGPRKRIPFTLPVALDLMLQIARGMEYLHSKKVYHGDLNPC 360

Query: 361 NILVKPRAYSTDGYVHAQVSGFGLPVVKFKNSSNQNESLPFIWYAPEVLEEQDQSGSAES 420
           NILVKPRAYSTDGYVH QVSGFGLP VKFKNSSNQNESLPFIWYAPEVLEEQDQSGSAES
Sbjct: 361 NILVKPRAYSTDGYVHGQVSGFGLPAVKFKNSSNQNESLPFIWYAPEVLEEQDQSGSAES 420

Query: 421 SKYTEKSDVYSFGMVCFEVLTGKVPFEDSHLQGDKMSRNIRAGERPLFPHSMPKYVTNLT 480
            KYTEKSDVYSFGMVCFEVLTGKVPFEDSHLQGDKMSRNIRAGERPLFPHSMPKYVTNLT
Sbjct: 421 CKYTEKSDVYSFGMVCFEVLTGKVPFEDSHLQGDKMSRNIRAGERPLFPHSMPKYVTNLT 480

Query: 481 KRCWHTDPNQRPSFPSICRILRYTKRFVAMNPDYNSQTDPAMPTVDYCDIESGLLRRLPS 540
           KRCW TDPNQRPSF SICRILRYTKRFVAMNPDYNSQTDPAMPTVDYCDIESGLLRRLPS
Sbjct: 481 KRCWQTDPNQRPSFTSICRILRYTKRFVAMNPDYNSQTDPAMPTVDYCDIESGLLRRLPS 540

Query: 541 CGISEASSAITDIPFQMFAYRIVEKEKAGANIKDTSESGSDASACGDETTSSVDDPFPTL 600
           CGIS+A+S ITDIPFQMFAYR+VEKE+AGA  KDTSESGSDASACGDET SS+DDPFPT 
Sbjct: 541 CGISDAASPITDIPFQMFAYRVVEKERAGATFKDTSESGSDASACGDETASSIDDPFPTP 600

Query: 601 VEKKLPVSREGTTRRLSLTKNNSDIKLNKLPGTPKGRFSRPPQISPRGRSMRMNSESQLM 660
           VE+KLP  REG +RRLSLTKNNSD++LNKLPGTPKGRFSRPPQISPRGRSMRMNSESQLM
Sbjct: 601 VERKLPAPREG-SRRLSLTKNNSDVRLNKLPGTPKGRFSRPPQISPRGRSMRMNSESQLM 660

Query: 661 AMSPKVRRLSGHASDSELP 680
           A+SPK+RRLSGHASDSELP
Sbjct: 661 AISPKIRRLSGHASDSELP 678

BLAST of Bhi01M000305 vs. NCBI nr
Match: XP_022147479.1 (mitogen-activated protein kinase kinase kinase 7-like isoform X1 [Momordica charantia])

HSP 1 Score: 1222.2 bits (3161), Expect = 0.0e+00
Identity = 604/683 (88.43%), Postives = 638/683 (93.41%), Query Frame = 0

Query: 1   MEQFRQLGEALGSVKALMLFKDSVHLNQRQCCLLLDFLSFAYDSVAEEMKQNLRFEEKHT 60
           MEQFRQLGEALGS+KALMLF+DS+H+NQRQC LL D L FAYDSVAEEMKQNLRFEEKHT
Sbjct: 1   MEQFRQLGEALGSLKALMLFRDSIHVNQRQCSLLFDVLCFAYDSVAEEMKQNLRFEEKHT 60

Query: 61  RWKVLDQPLRDLNRVFKEAEWYIRQCLETKDWWAKAIMLYQNTDCIELHIHNLLYSITVV 120
           RWKVLD PLRDLNRVFKEAEWYIRQCLETKDWWAKAIMLYQNTDCIE HIHNLLY I+VV
Sbjct: 61  RWKVLDLPLRDLNRVFKEAEWYIRQCLETKDWWAKAIMLYQNTDCIEFHIHNLLYCISVV 120

Query: 121 VEAIETAGENSGSDHDDMLKKRLINSIKYRREHKDLKIFKWKFGKQYLITQDFCNRMDAV 180
           VEA+ETAGE SG DH +M KKR+INS KYR E+KDLKIF+WK+GKQYLITQDFCNR+DAV
Sbjct: 121 VEALETAGEISGCDHHEMAKKRIINSTKYRNENKDLKIFRWKYGKQYLITQDFCNRVDAV 180

Query: 181 WNEDRWVLLNKIREKKFTASSKYEQRLTDLLLKNLDGPESLNGKLLPSSMLVGSRDYQVR 240
           W EDRWVLL+KIREKKFTASSKYEQRLTDLLLKNLDG ESLNGKLLPSSMLVGSRDYQVR
Sbjct: 181 WKEDRWVLLSKIREKKFTASSKYEQRLTDLLLKNLDGLESLNGKLLPSSMLVGSRDYQVR 240

Query: 241 RRLGGGSQYKEILWLGESFAMRHFFGEIESLIPEISMLLSLSHPNITRFLCGFTDEEKKE 300
           RRLGGGSQYKEILWLGESFAM+HFFGEIESLIPEIS LLS SHPNITRFLCGFTDEEKKE
Sbjct: 241 RRLGGGSQYKEILWLGESFAMKHFFGEIESLIPEISTLLSRSHPNITRFLCGFTDEEKKE 300

Query: 301 CFLIMELMSRDLSSYVKEICGPRKRMPFPLPVALDLMLQIARGMEYLHSKKIYHGDLNPC 360
           CFLIMELMSRDLSSYVKEICGPRKR+PF LPVALDLMLQIARGMEYLHS KIYHG+LNPC
Sbjct: 301 CFLIMELMSRDLSSYVKEICGPRKRIPFTLPVALDLMLQIARGMEYLHSNKIYHGNLNPC 360

Query: 361 NILVKPRAYSTDGYVHAQVSGFGLPVVKFKNSSNQNESLPFIWYAPEVLEEQDQSGSAES 420
           NIL+KPRAYSTDGYVHAQVSGFGLPVVKFKNSSNQNE+LPFIW++PEVLEEQDQSG+ ES
Sbjct: 361 NILIKPRAYSTDGYVHAQVSGFGLPVVKFKNSSNQNEALPFIWHSPEVLEEQDQSGTTES 420

Query: 421 SKYTEKSDVYSFGMVCFEVLTGKVPFEDSHLQGDKMSRNIRAGERPLFPHSMPKYVTNLT 480
           SKYTEKSDVYSFGMVCFEVLTGKVPFEDSHLQGDKMSRNIRAGERPLFPHSMPKYVTNLT
Sbjct: 421 SKYTEKSDVYSFGMVCFEVLTGKVPFEDSHLQGDKMSRNIRAGERPLFPHSMPKYVTNLT 480

Query: 481 KRCWHTDPNQRPSFPSICRILRYTKRFVAMNPDYNSQTDPAMPTVDYCDIESGLLRRL-P 540
           KRCWHTDPNQRPSF SICRILRYTKRFVA++PDYNSQTDPA P VDYCDIESGLLR+L P
Sbjct: 481 KRCWHTDPNQRPSFSSICRILRYTKRFVALHPDYNSQTDPATPIVDYCDIESGLLRKLPP 540

Query: 541 SCGISEASSAITDIPFQMFAYRIVEKEKAG-ANIKDTSESGSD-ASACGDETTSSVDDPF 600
           S G  +ASS I DIPFQMF YRIVEK+K+G ++ KDTSESGSD ASACGDE+ SSVDDPF
Sbjct: 541 SWGRCDASSTILDIPFQMFVYRIVEKQKSGTSSTKDTSESGSDGASACGDESASSVDDPF 600

Query: 601 PTLVEKKLPVSREG--TTRRLSLTKNNSDIKLNKLPGTPKGRFSRPPQISPRGRSMRMNS 660
            T+ EK+LP  REG  T RRLSLTKN+SD KLNKLPGTPKGR SRPPQISPRGRSMRMNS
Sbjct: 601 QTVTEKRLPAPREGMSTGRRLSLTKNSSDNKLNKLPGTPKGRSSRPPQISPRGRSMRMNS 660

Query: 661 ESQLMAMSPKVRRLSGHASDSEL 679
           ESQLMA+SPKVRR  GHASDSEL
Sbjct: 661 ESQLMALSPKVRRSCGHASDSEL 683

BLAST of Bhi01M000305 vs. NCBI nr
Match: XP_022933870.1 (light-sensor Protein kinase-like [Cucurbita moschata])

HSP 1 Score: 1214.1 bits (3140), Expect = 0.0e+00
Identity = 596/682 (87.39%), Postives = 631/682 (92.52%), Query Frame = 0

Query: 1   MEQFRQLGEALGSVKALMLFKDSVHLNQRQCCLLLDFLSFAYDSVAEEMKQNLRFEEKHT 60
           MEQFRQLGEALGSVKA+MLFKDS+ +NQRQCC LLD LSFAYDS+AEEM QNLRFEE+HT
Sbjct: 8   MEQFRQLGEALGSVKAIMLFKDSIQINQRQCCFLLDVLSFAYDSIAEEMNQNLRFEERHT 67

Query: 61  RWKVLDQPLRDLNRVFKEAEWYIRQCLETKDWWAKAIMLYQNTDCIELHIHNLLYSITVV 120
           RWKVLDQPLRDLNRVFKEAE +IRQCLE KDWW KA+M YQN DCIE H+HNLLYSITVV
Sbjct: 68  RWKVLDQPLRDLNRVFKEAEGFIRQCLEAKDWWVKALMQYQNNDCIEFHVHNLLYSITVV 127

Query: 121 VEAIETAGENSGSDHDDMLKKRLINSIKYRREHKDLKIFKWKFGKQYLITQDFCNRMDAV 180
           VEAIETAGENSG DHDDM+KKRLINS KYR+EHKD KIF+WKFGKQYLITQ+FCNR+D V
Sbjct: 128 VEAIETAGENSGFDHDDMMKKRLINSFKYRKEHKDSKIFRWKFGKQYLITQEFCNRIDTV 187

Query: 181 WNEDRWVLLNKIREKKFTASSKYEQRLTDLLLKNLDGPESLNGKLLPSSMLVGSRDYQVR 240
           WNEDRW LLN+IREKK TASS+YEQRLTD LLKNLDG E L GKLLPSSMLVGSRDYQVR
Sbjct: 188 WNEDRWFLLNRIREKKLTASSRYEQRLTDHLLKNLDGSECLIGKLLPSSMLVGSRDYQVR 247

Query: 241 RRLGGGSQYKEILWLGESFAMRHFFGEIESLIPEISMLLSLSHPNITRFLCGFTDEEKKE 300
           RRLGGGSQYKEILWLGESFAMRHFFGEIE+LIPEIS+LLSLSHPNITRFLCGFTDEEKKE
Sbjct: 248 RRLGGGSQYKEILWLGESFAMRHFFGEIEALIPEISILLSLSHPNITRFLCGFTDEEKKE 307

Query: 301 CFLIMELMSRDLSSYVKEICGPRKRMPFPLPVALDLMLQIARGMEYLHSKKIYHGDLNPC 360
           CFLIMELMSRDLSSYVKEICG RKR+PFPLPVALDLMLQIARGMEYLHSKKI+HGDLNP 
Sbjct: 308 CFLIMELMSRDLSSYVKEICGYRKRIPFPLPVALDLMLQIARGMEYLHSKKIFHGDLNPY 367

Query: 361 NILVKPRAYSTDGYVHAQVSGFGLPVVKFKNSSNQNESLPFIWYAPEVLEEQDQSGSAES 420
           NILVKPRA STDGYVHAQVSGFGLPVVKFKNSSNQNESLPFIWYAPEVLEEQDQSGS  S
Sbjct: 368 NILVKPRACSTDGYVHAQVSGFGLPVVKFKNSSNQNESLPFIWYAPEVLEEQDQSGSTPS 427

Query: 421 SKYTEKSDVYSFGMVCFEVLTGKVPFEDSHLQGDKMSRNIRAGERPLFPHSMPKYVTNLT 480
            KY+EKSDVYSFGMVCFE+LTGKVPFEDSHLQGDKMSRNIRAGERPLFPHSMPKYVTNLT
Sbjct: 428 CKYSEKSDVYSFGMVCFELLTGKVPFEDSHLQGDKMSRNIRAGERPLFPHSMPKYVTNLT 487

Query: 481 KRCWHTDPNQRPSFPSICRILRYTKRFVAMNPDYNSQTDPAMPTVDYCDIESGLLRRLPS 540
           KRCWHTDPNQRP F SICRILRY KRFVAM+PDY+SQTDPA P VDYCDIES LLR+L  
Sbjct: 488 KRCWHTDPNQRPGFSSICRILRYVKRFVAMHPDYSSQTDPATPAVDYCDIESCLLRKLSR 547

Query: 541 CGISEASSAITDIPFQMFAYRIVEKEKAGAN--IKDTSESGSD-ASACGDETTSSVDDPF 600
            G    SSAI DIPFQMF +RIVEKEK G +  +KDTSESGSD ASACGDET SS D+PF
Sbjct: 548 WG----SSAIVDIPFQMFVFRIVEKEKTGGSSVVKDTSESGSDGASACGDETASSTDEPF 607

Query: 601 PTLVEKKLPVSREGTTRRLSLTKNNSDIKLNKLPGTPKGRFSRPPQISPRGRSMRMNSES 660
           PTLVEK+LPVSRE   RRLSL+KN+SD+KLNKLPGTP+GRFSRPPQISPRGRSMRMNSES
Sbjct: 608 PTLVEKRLPVSRESINRRLSLSKNSSDVKLNKLPGTPQGRFSRPPQISPRGRSMRMNSES 667

Query: 661 QLMAMSPKVRRLSGHASDSELP 680
           QL+AM+PKVRRLSGHASDSELP
Sbjct: 668 QLIAMTPKVRRLSGHASDSELP 685

BLAST of Bhi01M000305 vs. NCBI nr
Match: XP_023527513.1 (mitogen-activated protein kinase kinase kinase 7-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1213.4 bits (3138), Expect = 0.0e+00
Identity = 601/683 (87.99%), Postives = 632/683 (92.53%), Query Frame = 0

Query: 1   MEQFRQLGEALGSVKALMLFKDSVHLNQRQCCLLLDFLSFAYDSVAEEMKQNLRFEEKHT 60
           MEQFRQLGEALGSVKALMLFKDSV +NQRQCCLLLD L FAYDSVAEEMKQNLRFEEKHT
Sbjct: 1   MEQFRQLGEALGSVKALMLFKDSVRINQRQCCLLLDVLGFAYDSVAEEMKQNLRFEEKHT 60

Query: 61  RWKVLDQPLRDLNRVFKEAEWYIRQCLETKDWWAKAIMLYQNTDCIELHIHNLLYSITVV 120
           RWKVL+QPLRDLNRVFKEAE YIRQCLE KD WAKAI+LYQNTDCIE HIHNLLYSI VV
Sbjct: 61  RWKVLEQPLRDLNRVFKEAEGYIRQCLEVKDRWAKAIILYQNTDCIEFHIHNLLYSIAVV 120

Query: 121 VEAIETAGENSGSDHDDMLKKRLINSIKYRREHKDLKIFKWKFGKQYLITQDFCNRMDAV 180
           VEAIETAGENSG D D+MLKKRLINSIKYRREHKDLK FKWKFGKQYLIT+DFCNR+DAV
Sbjct: 121 VEAIETAGENSGCDQDEMLKKRLINSIKYRREHKDLKTFKWKFGKQYLITRDFCNRVDAV 180

Query: 181 WNEDRWVLLNKIREKKFTASSKYEQRLTDLLLKNLDGPESLNGKLLPSSMLVGSRDYQVR 240
           WNEDRWVLLNK+RE KF+A+SKYEQRLTD LLKN+DG ESLNGKLLPSSMLVGSRDYQVR
Sbjct: 181 WNEDRWVLLNKMREMKFSAASKYEQRLTDHLLKNIDGSESLNGKLLPSSMLVGSRDYQVR 240

Query: 241 RRLGGGSQYKEILWLGESFAMRHFFGEIESLIPEISMLLSLSHPNITRFLCGFTDEEKKE 300
           RRLGGGS+YK+ILWLGESFAMRHFFGEIE+L+PEISMLLSLSHPNITRFLCGFTDEEKKE
Sbjct: 241 RRLGGGSKYKDILWLGESFAMRHFFGEIETLLPEISMLLSLSHPNITRFLCGFTDEEKKE 300

Query: 301 CFLIMELMSRDLSSYVKEICGPRKRMPFPLPVALDLMLQIARGMEYLHSKKIYHGDLNPC 360
           CFLIMELMSRDLSSY+KEICG RKR PFPLPVALDLMLQIARGMEYLHSKKIYHGDLNPC
Sbjct: 301 CFLIMELMSRDLSSYIKEICGSRKRFPFPLPVALDLMLQIARGMEYLHSKKIYHGDLNPC 360

Query: 361 NILVKPRAYSTDGYVHAQVSGFGLPVVKFKNSSNQNESLPFIWYAPEVLEEQDQSGSAES 420
           NILVKPRA STDGYVHAQVSGFGLPVV+FK SSNQNES PFIWYAPEVLEEQ QSGSAES
Sbjct: 361 NILVKPRACSTDGYVHAQVSGFGLPVVEFKKSSNQNESRPFIWYAPEVLEEQGQSGSAES 420

Query: 421 SKYTEKSDVYSFGMVCFEVLTGKVPFEDSHLQGDKMSRNIRAGERPLFPHSMPKYVTNLT 480
           SKYTEKSDVYSF MVCFE+LTGKVPFEDSHLQGDKMSRNIRAGERPLFPHSMPKYVTNLT
Sbjct: 421 SKYTEKSDVYSFAMVCFELLTGKVPFEDSHLQGDKMSRNIRAGERPLFPHSMPKYVTNLT 480

Query: 481 KRCWHTDPNQRPSFPSICRILRYTKRFVAMNPDYNSQTDPAMPTVDYCDIESGLLRRLPS 540
           KRCWHTDPNQRPSFPSICRILRY KRFVAMNPDYNSQTDPA P VDYCDIESGLLR LPS
Sbjct: 481 KRCWHTDPNQRPSFPSICRILRYIKRFVAMNPDYNSQTDPATPIVDYCDIESGLLRNLPS 540

Query: 541 CGISEASSAITDIPFQMFAYRIVEKEKAGANIKDTSESGSDASACGDETTSSVDDPFPTL 600
           CGIS+ASSAITDIPFQMF YRIVEKEK G +IKDTSESGS  S   DETT+S+D+P PT 
Sbjct: 541 CGISDASSAITDIPFQMFVYRIVEKEKDGTSIKDTSESGS--SDGSDETTTSIDEPLPTP 600

Query: 601 VE--KKLPVSREGTTRRLSLTKNNSDIKLNKLP--GTPKGRFSRPPQISPRGRSMRMNSE 660
           +   K+L  SREGT RR SLTKNNSD +LNKL   GTPK +FSRPPQ  PRGR++RMNSE
Sbjct: 601 IPVIKRLQASREGTNRRPSLTKNNSDTRLNKLAGRGTPKEQFSRPPQNRPRGRTIRMNSE 660

Query: 661 SQLMAMSPKVRRLSGHASDSELP 680
           S+L+ +SPKVRR SGHASDSELP
Sbjct: 661 SKLIVLSPKVRRSSGHASDSELP 681