MELO3C032733.2 (gene) Melon (DHL92) v3.6.1

Overview
NameMELO3C032733.2
Typegene
OrganismCucumis melo (Melon (DHL92) v3.6.1)
DescriptiontRNA-specific 2-thiouridylase MnmA
Locationchr07 : 15927149 .. 15929621 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGCAAATTGGAAAAGGAAAAAATGAAAAGGATCAAAGCAACAGTGACTGTTTCATAGTGGATCACCTGGGCACTCACAAACTTGTTTGTGAAGTGCATCTTCAAGACATGGCTTTTAGATACTTGGTTATGGACATATGGAATGCAATTTTAGATGGATGTGCTATAGATTGTAATGTCAAGATATGTTAAACTTGAATATTATGTGATGACTTTTGACAAGATATGTTCAATTTTTTAACCGGGTTTTTAGCATATTGAAATATGCAACTGTGCAAGCAGAGTTTTTTACTCATGTTGATTATATAACACTGTTGTTGTATATTTCCATGATGTTTTATTACAAAAAAACTTGTATGCATAAAAATTGTTTGTAATACATATCCGATCCCTTATACTCAACTACATACGTAGTGGATAAAGGTCTTCAAGCATAAAATAGGCATCTTAAGTTTTATGCTATTGGTTGAATTGAACTTGATCGTCGTTTGAATTGCTCGGTAGAACCTGTAGGACTACATTAAGATGGGGTTCTACCCTTTTTCTTCGGCCATTTCAAATTTCATTAGAACTAAGATATCATCCTAGGACATGCGTTTTAAGTTCAGGGTTTTTGTTATTGATTGAACTTGACAGCCTTTTACTAGCTTTTGAATTGCTCAGTTGAACTTGTATGGTTTCAGAATTTGCAACAGGATATTACACTTCAAAAATTATTGTAGTTGATATAGTGAATGCTGGCGTAATTGTGAGTAATCAAATGGCCATTTGAGCTCGAAGGTAATAGCCTTTTCGTGTGAATGCACTTAAATGGGTTTTGTTTTCCCAGGAAGACTTCGAGAAATTTTTGTCAGAATGTTCATGCGACGATGATTTGAAGTATGCTAAAGCTGTTTGTGATCATGTTTGTTACTATTTTAGCTTATAAATTCATCTAGACTTACAAAGGTTAATTTTTCCAATGAATATTCAAATGAAATAAAGGATTTGTTGGGAAAATTAAACATAATTCAAGATACCCACAAGTAATGTCTTTCTTCTGTAGTTAGTATTGTCTTTCTTTTGTTGGAAAAATTAAACACTATTTTGCTATTTTCATGACTTTTGTCTATATTCTACACTACTCATGGTAGTTAGTAAGGCCTTTAGTAATTTAACTTGAAGTTTCATGTATAAGCTCAGTGGAAATAGATATGACTAATATTGCATTATTCGGCCAATATTTGTTGCAATCCTTTAGGAAACAACATCATTAATAAGCTTTATGATTTATGTATCACTTATCTATTCAAGGTTTATCATCAAACACATTTATCCCGTCCTTATTCCTTCTTGCTCCAAAATAAAGATTTCTTATGTTACTTGAATATTATTGTTCTTAGGTCAAGGATCAAACATATTTTCTATCACATCTCTCACAGAATCAACTTAAAAGGCTTTTATTCCCTCTTGGTTGTATTCCAAAGGTTTGTTCATGCTAAATTTATAGTCTTTATTATCATGTATTTTTTTTTTGAGGTATCTCTTATGTTTCAGACTTCAGGGTGTATTCCGAGAAGTTCAAGTTCAACTGTTGTAAATTTGTAAGATTTGGTACTAGCCTTCTAAGCTTGTACAGACATAAACACCTAGTAGCTAACATTGATTTCATTAAGTTTAGAAATTTATGTATTTAATAAAAGTTAAAAATTGTATTATAGACTTTGGCAAGCCAATTGATGTAGATAACATTTTAGAGATTTGTGGATGTTATATTGCAGTTTAAACCTATTAAGTTTTCACTATTCTCGTGTGCGAAACTTATATAAGTGCCTCAATTAATTTGTTCATATCATGCTAACTCCTATCCCAACTAGCTACATTCTTTCAACTTATGCTCTATCTTTTCGGTAAGGAAAAAGGAAAGGAAAAAGGAAAAAAGGAAAAAGGAAATACTTGTGTGATTGTTATCTTGTTAATATGTTTTTCTAGGATTGTGACTTTAGCAATATTTTTCTTCTCTCAACTTTATATTCCCTCAACAATCATTTGCAGGATGAATTTCGCAAGCTTGCTGTAAAATTCAACCTACCAAACAAAGACATAAAGGAATCACAAGGAATATGTATTTTGGTGATTCATACATATTTTTTGTTTGTTTGTCTTTTTCTTTTCAGGAGGCTAATGACTATCTCAAAATTCATTGTTTAATGCTTATCTATTTCTAATATATTGGCGTTGTTGTTTATATTTTAGAATATTCTTTACTACTTTTTAGAACTATTTATAGTAATACAGGATTTAGTACAAGACTTGCCAACTAGTGTTGAAATTTTCTGATGGCTATACGTTCCTTGAGCAAGTGTTTTATTTCCTTATTCTTCCTGACAGCCAACGCAAAGGTCTAAGGAAAAAGGAAAAAGACTTCCTGGAGGACCTTGGTATGCCTTCTCAGTTTCGTTTCTTTAAGAATAAATTTGATGATCTCTAA

mRNA sequence

ATGAAGCAAATTGGAAAAGGAAAAAATGAAAAGGATCAAAGCAACAGTGACTGTTTCATAGTGGATCACCTGGGCACTCACAAACTTGTTTGTGAAGTGCATCTTCAAGACATGGCTTTTAGATACTTGGTTATGGACATATGGAATGCAATTTTAGATGGATGTGCTATAGATTGTAATGAAGACTTCGAGAAATTTTTGTCAGAATGTTCATGCGACGATGATTTGAAGTATGCTAAAGCTGTTTGTGATCATGTCAAGGATCAAACATATTTTCTATCACATCTCTCACAGAATCAACTTAAAAGGCTTTTATTCCCTCTTGGTTGTATTCCAAAGGTATCTCTTATGTTTCAGACTTCAGGGTGTATTCCGAGAAGTTCAAGTTCAACTGTTGATTGTGACTTTAGCAATATTTTTCTTCTCTCAACTTTATATTCCCTCAACAATCATTTGCAGGATGAATTTCGCAAGCTTGCTGTAAAATTCAACCTACCAAACAAAGACATAAAGGAATCACAAGGAATATGTATTTTGTACAAGACTTGCCAACTAGTGTTGAAATTTTCTGATGGCTATACGTTCCTTGAGCAAGTGTTTTATTTCCTTATTCTTCCTGACAGCCAACGCAAAGGTCTAAGGAAAAAGGAAAAAGACTTCCTGGAGGACCTTGGTATGCCTTCTCAGTTTCGTTTCTTTAAGAATAAATTTGATGATCTCTAA

Coding sequence (CDS)

ATGAAGCAAATTGGAAAAGGAAAAAATGAAAAGGATCAAAGCAACAGTGACTGTTTCATAGTGGATCACCTGGGCACTCACAAACTTGTTTGTGAAGTGCATCTTCAAGACATGGCTTTTAGATACTTGGTTATGGACATATGGAATGCAATTTTAGATGGATGTGCTATAGATTGTAATGAAGACTTCGAGAAATTTTTGTCAGAATGTTCATGCGACGATGATTTGAAGTATGCTAAAGCTGTTTGTGATCATGTCAAGGATCAAACATATTTTCTATCACATCTCTCACAGAATCAACTTAAAAGGCTTTTATTCCCTCTTGGTTGTATTCCAAAGGTATCTCTTATGTTTCAGACTTCAGGGTGTATTCCGAGAAGTTCAAGTTCAACTGTTGATTGTGACTTTAGCAATATTTTTCTTCTCTCAACTTTATATTCCCTCAACAATCATTTGCAGGATGAATTTCGCAAGCTTGCTGTAAAATTCAACCTACCAAACAAAGACATAAAGGAATCACAAGGAATATGTATTTTGTACAAGACTTGCCAACTAGTGTTGAAATTTTCTGATGGCTATACGTTCCTTGAGCAAGTGTTTTATTTCCTTATTCTTCCTGACAGCCAACGCAAAGGTCTAAGGAAAAAGGAAAAAGACTTCCTGGAGGACCTTGGTATGCCTTCTCAGTTTCGTTTCTTTAAGAATAAATTTGATGATCTCTAA

Protein sequence

MKQIGKGKNEKDQSNSDCFIVDHLGTHKLVCEVHLQDMAFRYLVMDIWNAILDGCAIDCNEDFEKFLSECSCDDDLKYAKAVCDHVKDQTYFLSHLSQNQLKRLLFPLGCIPKVSLMFQTSGCIPRSSSSTVDCDFSNIFLLSTLYSLNNHLQDEFRKLAVKFNLPNKDIKESQGICILYKTCQLVLKFSDGYTFLEQVFYFLILPDSQRKGLRKKEKDFLEDLGMPSQFRFFKNKFDDL
Homology
[Close]
BLAST of MELO3C032733.2 vs. NCBI nr
Match: KRH29713.1 (hypothetical protein GLYMA_11G133500 [Glycine max])

HSP 1 Score: 99.8 bits (247), Expect = 1.4e-17
Identity = 62/138 (44.93%), Postives = 69/138 (50.00%), Query Frame = 0 

Query: 62  DFEKFLSECSCDDDLKYAKAVCDH------------------VKDQTYFLSHLSQNQLKR 121
           +FE F SEC  +DDLKYAK VC+                   VKDQTYFLSHLSQ+QLK+
Sbjct: 4   NFENFWSECPWEDDLKYAKDVCNQVDVPLEVVHLTDEYWNNVVKDQTYFLSHLSQSQLKQ 63

Query: 122 LLFPLGCIPKVSLMFQTSGCIPRSSSSTVDCDFSNIFLLSTLYSLNNHLQDEFRKLAVKF 181
           LLFPLGCIPK                                        DE RKLA KF
Sbjct: 64  LLFPLGCIPK----------------------------------------DEVRKLATKF 101

[Close]
BLAST of MELO3C032733.2 vs. NCBI nr
Match: XP_004142116.1 (PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1 [Cucumis sativus] >KGN54175.1 hypothetical protein Csa_4G291390 [Cucumis sativus])

HSP 1 Score: 89.4 bits (220), Expect = 1.9e-14
Identity = 73/218 (33.49%), Postives = 76/218 (34.86%), Query Frame = 0 

Query: 61  EDFEKFLSECSCDDDLKYAKAVCDH----------------------------------- 120
           EDFE F SEC  DDDLKYAKAVCD                                    
Sbjct: 119 EDFENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPD 178

Query: 121 ----------------------------------------------------VKDQTYFL 180
                                                               VKDQTYFL
Sbjct: 179 VLCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFL 238

Query: 181 SHLSQNQLKRLLFPLGCIPKVSLMFQTSGCIPRSSSSTVDCDFSNIFLLSTLYSLNNHLQ 192
           SHLSQNQLKRLLFPLGCIPK                                        
Sbjct: 239 SHLSQNQLKRLLFPLGCIPK---------------------------------------- 291

[Close]
BLAST of MELO3C032733.2 vs. NCBI nr
Match: XP_008449720.1 (PREDICTED: tRNA-specific 2-thiouridylase MnmA [Cucumis melo])

HSP 1 Score: 89.4 bits (220), Expect = 1.9e-14
Identity = 73/218 (33.49%), Postives = 76/218 (34.86%), Query Frame = 0 

Query: 61  EDFEKFLSECSCDDDLKYAKAVCDH----------------------------------- 120
           EDFE F SEC  DDDLKYAKAVCD                                    
Sbjct: 120 EDFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPD 179

Query: 121 ----------------------------------------------------VKDQTYFL 180
                                                               VKDQTYFL
Sbjct: 180 VLCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFL 239

Query: 181 SHLSQNQLKRLLFPLGCIPKVSLMFQTSGCIPRSSSSTVDCDFSNIFLLSTLYSLNNHLQ 192
           SHLSQNQLKRLLFPLGCIPK                                        
Sbjct: 240 SHLSQNQLKRLLFPLGCIPK---------------------------------------- 292

[Close]
BLAST of MELO3C032733.2 vs. NCBI nr
Match: XP_022944568.1 (uncharacterized protein LOC111448983 [Cucurbita moschata])

HSP 1 Score: 89.4 bits (220), Expect = 1.9e-14
Identity = 73/218 (33.49%), Postives = 76/218 (34.86%), Query Frame = 0 

Query: 61  EDFEKFLSECSCDDDLKYAKAVCDH----------------------------------- 120
           EDFE F SEC  DDDLKYAKAVCD                                    
Sbjct: 117 EDFENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPD 176

Query: 121 ----------------------------------------------------VKDQTYFL 180
                                                               VKDQTYFL
Sbjct: 177 VLCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMEKPSVLELSKDMVKDQTYFL 236

Query: 181 SHLSQNQLKRLLFPLGCIPKVSLMFQTSGCIPRSSSSTVDCDFSNIFLLSTLYSLNNHLQ 192
           SHLSQNQLKRLLFPLGCIPK                                        
Sbjct: 237 SHLSQNQLKRLLFPLGCIPK---------------------------------------- 289

[Close]
BLAST of MELO3C032733.2 vs. NCBI nr
Match: XP_022985745.1 (uncharacterized protein LOC111483706 [Cucurbita maxima])

HSP 1 Score: 89.4 bits (220), Expect = 1.9e-14
Identity = 73/218 (33.49%), Postives = 76/218 (34.86%), Query Frame = 0 

Query: 61  EDFEKFLSECSCDDDLKYAKAVCDH----------------------------------- 120
           EDFE F SEC  DDDLKYAKAVCD                                    
Sbjct: 117 EDFENFWSECPWDDDLKYAKAVCDRVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPD 176

Query: 121 ----------------------------------------------------VKDQTYFL 180
                                                               VKDQTYFL
Sbjct: 177 VLCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFL 236

Query: 181 SHLSQNQLKRLLFPLGCIPKVSLMFQTSGCIPRSSSSTVDCDFSNIFLLSTLYSLNNHLQ 192
           SHLSQNQLKRLLFPLGCIPK                                        
Sbjct: 237 SHLSQNQLKRLLFPLGCIPK---------------------------------------- 289

[Close]
BLAST of MELO3C032733.2 vs. TAIR10
Match: AT1G51310.1 (transferases;tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferases)

HSP 1 Score: 80.1 bits (196), Expect = 2.0e-15
Identity = 50/108 (46.30%), Postives = 55/108 (50.93%), Query Frame = 0 

Query: 84  DHVKDQTYFLSHLSQNQLKRLLFPLGCIPKVSLMFQTSGCIPRSSSSTVDCDFSNIFLLS 143
           D VKDQTYFLSHLSQ QLKRLLFPLGC+ K                              
Sbjct: 229 DMVKDQTYFLSHLSQTQLKRLLFPLGCVKK------------------------------ 288

Query: 144 TLYSLNNHLQDEFRKLAVKFNLPNKDIKESQGICILYKTCQLVLKFSD 192
                     DE RKLA +F+LPNKD K+SQGIC L K     +KFSD
Sbjct: 289 ----------DEVRKLATQFDLPNKDRKDSQGICFLGK-----IKFSD 291

[Close]
BLAST of MELO3C032733.2 vs. Swiss-Prot
Match: sp|O51625|MNMA_BORBU (tRNA-specific 2-thiouridylase MnmA OS=Borrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680) OX=224326 GN=mnmA PE=3 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 1.8e-08
Identity = 40/106 (37.74%), Postives = 49/106 (46.23%), Query Frame = 0 

Query: 76  LKYAKAVCDHVKDQTYFLSHLSQNQLKRLLFPLGCIPKVSLMFQTSGCIPRSSSSTVDCD 135
           LK AK   D +KDQ+YFLSHLSQ Q+ +L FPLG +                        
Sbjct: 137 LKQAK---DKIKDQSYFLSHLSQKQMSKLYFPLGTL------------------------ 196

Query: 136 FSNIFLLSTLYSLNNHLQDEFRKLAVKFNLPNKDIKESQGICILYK 182
                           L+ E R++A   NLPNKD K+SQGIC L K
Sbjct: 197 ----------------LKSEVRQIAKNINLPNKDRKDSQGICFLGK 199

[Close]
BLAST of MELO3C032733.2 vs. Swiss-Prot
Match: sp|B7J2N7|MNMA_BORBZ (tRNA-specific 2-thiouridylase MnmA OS=Borrelia burgdorferi (strain ZS7) OX=445985 GN=mnmA PE=3 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 1.8e-08
Identity = 40/106 (37.74%), Postives = 49/106 (46.23%), Query Frame = 0 

Query: 76  LKYAKAVCDHVKDQTYFLSHLSQNQLKRLLFPLGCIPKVSLMFQTSGCIPRSSSSTVDCD 135
           LK AK   D +KDQ+YFLSHLSQ Q+ +L FPLG +                        
Sbjct: 137 LKQAK---DKIKDQSYFLSHLSQKQISKLYFPLGTL------------------------ 196

Query: 136 FSNIFLLSTLYSLNNHLQDEFRKLAVKFNLPNKDIKESQGICILYK 182
                           L+ E R++A   NLPNKD K+SQGIC L K
Sbjct: 197 ----------------LKSEVRQIAKNINLPNKDRKDSQGICFLGK 199

[Close]
BLAST of MELO3C032733.2 vs. Swiss-Prot
Match: sp|Q660I8|MNMA_BORBP (tRNA-specific 2-thiouridylase MnmA OS=Borreliella bavariensis (strain ATCC BAA-2496 / DSM 23469 / PBi) OX=290434 GN=mnmA PE=3 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 5.2e-08
Identity = 36/104 (34.62%), Postives = 46/104 (44.23%), Query Frame = 0 

Query: 78  YAKAVCDHVKDQTYFLSHLSQNQLKRLLFPLGCIPKVSLMFQTSGCIPRSSSSTVDCDFS 137
           + K   D +KDQ+YFLSHLSQ Q+ +L FPLG +                          
Sbjct: 136 FLKQAKDKIKDQSYFLSHLSQEQMSKLYFPLGTL-------------------------- 195

Query: 138 NIFLLSTLYSLNNHLQDEFRKLAVKFNLPNKDIKESQGICILYK 182
                          + E R++A   NLPNKD K+SQGIC L K
Sbjct: 196 --------------FKSEVRQIAKSINLPNKDRKDSQGICFLGK 199

[Close]
BLAST of MELO3C032733.2 vs. Swiss-Prot
Match: sp|A1R0A7|MNMA_BORT9 (tRNA-specific 2-thiouridylase MnmA OS=Borrelia turicatae (strain 91E135) OX=314724 GN=mnmA PE=3 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 5.7e-07
Identity = 38/106 (35.85%), Postives = 50/106 (47.17%), Query Frame = 0 

Query: 76  LKYAKAVCDHVKDQTYFLSHLSQNQLKRLLFPLGCIPKVSLMFQTSGCIPRSSSSTVDCD 135
           LK AK   D +KDQ+YFLSHLS+ Q+ +L FPLG +                        
Sbjct: 137 LKQAK---DKIKDQSYFLSHLSRKQISKLHFPLGDL------------------------ 196

Query: 136 FSNIFLLSTLYSLNNHLQDEFRKLAVKFNLPNKDIKESQGICILYK 182
                           L+ E R++A K +LPNK+ K+SQGIC L K
Sbjct: 197 ----------------LKTEIRQIAQKIDLPNKNRKDSQGICFLGK 199

[Close]
BLAST of MELO3C032733.2 vs. Swiss-Prot
Match: sp|B2S125|MNMA_BORHD (tRNA-specific 2-thiouridylase MnmA OS=Borrelia hermsii (strain HS1 / DAH) OX=314723 GN=mnmA PE=3 SV=2)

HSP 1 Score: 55.8 bits (133), Expect = 7.5e-07
Identity = 38/106 (35.85%), Postives = 50/106 (47.17%), Query Frame = 0 

Query: 76  LKYAKAVCDHVKDQTYFLSHLSQNQLKRLLFPLGCIPKVSLMFQTSGCIPRSSSSTVDCD 135
           LK AK   D +KDQ+YFLSHLS+ Q+ +L FPLG +                        
Sbjct: 137 LKQAK---DKIKDQSYFLSHLSREQISKLHFPLGDL------------------------ 196

Query: 136 FSNIFLLSTLYSLNNHLQDEFRKLAVKFNLPNKDIKESQGICILYK 182
                           L+ E R++A K +LPNK+ K+SQGIC L K
Sbjct: 197 ----------------LKTEIRQIAHKIDLPNKNRKDSQGICFLGK 199

[Close]
BLAST of MELO3C032733.2 vs. TrEMBL
Match: tr|K7LPK6|K7LPK6_SOYBN (Uncharacterized protein OS=Glycine max OX=3847 GN=100785275 PE=4 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 9.1e-18
Identity = 62/138 (44.93%), Postives = 69/138 (50.00%), Query Frame = 0 

Query: 62  DFEKFLSECSCDDDLKYAKAVCDH------------------VKDQTYFLSHLSQNQLKR 121
           +FE F SEC  +DDLKYAK VC+                   VKDQTYFLSHLSQ+QLK+
Sbjct: 4   NFENFWSECPWEDDLKYAKDVCNQVDVPLEVVHLTDEYWNNVVKDQTYFLSHLSQSQLKQ 63

Query: 122 LLFPLGCIPKVSLMFQTSGCIPRSSSSTVDCDFSNIFLLSTLYSLNNHLQDEFRKLAVKF 181
           LLFPLGCIPK                                        DE RKLA KF
Sbjct: 64  LLFPLGCIPK----------------------------------------DEVRKLATKF 101

[Close]
BLAST of MELO3C032733.2 vs. TrEMBL
Match: tr|A0A1S3BNL8|A0A1S3BNL8_CUCME (tRNA-specific 2-thiouridylase MnmA OS=Cucumis melo OX=3656 GN=LOC103491516 PE=3 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 1.2e-14
Identity = 73/218 (33.49%), Postives = 76/218 (34.86%), Query Frame = 0 

Query: 61  EDFEKFLSECSCDDDLKYAKAVCDH----------------------------------- 120
           EDFE F SEC  DDDLKYAKAVCD                                    
Sbjct: 120 EDFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPD 179

Query: 121 ----------------------------------------------------VKDQTYFL 180
                                                               VKDQTYFL
Sbjct: 180 VLCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFL 239

Query: 181 SHLSQNQLKRLLFPLGCIPKVSLMFQTSGCIPRSSSSTVDCDFSNIFLLSTLYSLNNHLQ 192
           SHLSQNQLKRLLFPLGCIPK                                        
Sbjct: 240 SHLSQNQLKRLLFPLGCIPK---------------------------------------- 292

[Close]
BLAST of MELO3C032733.2 vs. TrEMBL
Match: tr|A0A0A0KX16|A0A0A0KX16_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G291390 PE=3 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 1.2e-14
Identity = 73/218 (33.49%), Postives = 76/218 (34.86%), Query Frame = 0 

Query: 61  EDFEKFLSECSCDDDLKYAKAVCDH----------------------------------- 120
           EDFE F SEC  DDDLKYAKAVCD                                    
Sbjct: 119 EDFENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPD 178

Query: 121 ----------------------------------------------------VKDQTYFL 180
                                                               VKDQTYFL
Sbjct: 179 VLCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFL 238

Query: 181 SHLSQNQLKRLLFPLGCIPKVSLMFQTSGCIPRSSSSTVDCDFSNIFLLSTLYSLNNHLQ 192
           SHLSQNQLKRLLFPLGCIPK                                        
Sbjct: 239 SHLSQNQLKRLLFPLGCIPK---------------------------------------- 291

[Close]
BLAST of MELO3C032733.2 vs. TrEMBL
Match: tr|A0A2I0JXK8|A0A2I0JXK8_PUNGR (Uncharacterized protein OS=Punica granatum OX=22663 GN=CRG98_019384 PE=3 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 1.8e-13
Identity = 55/123 (44.72%), Postives = 63/123 (51.22%), Query Frame = 0 

Query: 69  ECSCDDDLKYAKAVCDHVKDQTYFLSHLSQNQLKRLLFPLGCIPKVSLMFQTSGCIPRSS 128
           E  C   L+ +K   D +KDQTYFLSHLSQ QLKRL+FPLGC+PK               
Sbjct: 142 EADCPSVLELSK---DVIKDQTYFLSHLSQTQLKRLVFPLGCMPK--------------- 201

Query: 129 SSTVDCDFSNIFLLSTLYSLNNHLQDEFRKLAVKFNLPNKDIKESQGICILYKTCQLVLK 188
                                    DE RKLAVKFNLPNK+ K+SQGIC L K     +K
Sbjct: 202 -------------------------DEVRKLAVKFNLPNKERKDSQGICFLGK-----IK 216

Query: 189 FSD 192
           FSD
Sbjct: 262 FSD 216

[Close]
BLAST of MELO3C032733.2 vs. TrEMBL
Match: tr|A0A218WLN9|A0A218WLN9_PUNGR (Uncharacterized protein OS=Punica granatum OX=22663 GN=CDL15_Pgr026592 PE=3 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 1.8e-13
Identity = 55/123 (44.72%), Postives = 63/123 (51.22%), Query Frame = 0 

Query: 69  ECSCDDDLKYAKAVCDHVKDQTYFLSHLSQNQLKRLLFPLGCIPKVSLMFQTSGCIPRSS 128
           E  C   L+ +K   D +KDQTYFLSHLSQ QLKRL+FPLGC+PK               
Sbjct: 213 EADCPSVLELSK---DVIKDQTYFLSHLSQTQLKRLVFPLGCMPK--------------- 272

Query: 129 SSTVDCDFSNIFLLSTLYSLNNHLQDEFRKLAVKFNLPNKDIKESQGICILYKTCQLVLK 188
                                    DE RKLAVKFNLPNK+ K+SQGIC L K     +K
Sbjct: 273 -------------------------DEVRKLAVKFNLPNKERKDSQGICFLGK-----IK 287

Query: 189 FSD 192
           FSD
Sbjct: 333 FSD 287

The following BLAST results are available for this feature:
BLAST of MELO3C032733.2 vs. NCBI nr
Match NameE-valueIdentityDescription
KRH29713.11.4e-1744.93hypothetical protein GLYMA_11G133500 [Glycine max][more]
XP_004142116.11.9e-1433.49PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1 [Cucumis sativus] >KGN5... [more]
XP_008449720.11.9e-1433.49PREDICTED: tRNA-specific 2-thiouridylase MnmA [Cucumis melo][more]
XP_022944568.11.9e-1433.49uncharacterized protein LOC111448983 [Cucurbita moschata][more]
XP_022985745.11.9e-1433.49uncharacterized protein LOC111483706 [Cucurbita maxima][more]
BLAST of MELO3C032733.2 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G51310.12.0e-1546.30transferases;tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferases[more]
BLAST of MELO3C032733.2 vs. Swiss-Prot
Match NameE-valueIdentityDescription
sp|O51625|MNMA_BORBU1.8e-0837.74tRNA-specific 2-thiouridylase MnmA OS=Borrelia burgdorferi (strain ATCC 35210 / ... [more]
sp|B7J2N7|MNMA_BORBZ1.8e-0837.74tRNA-specific 2-thiouridylase MnmA OS=Borrelia burgdorferi (strain ZS7) OX=44598... [more]
sp|Q660I8|MNMA_BORBP5.2e-0834.62tRNA-specific 2-thiouridylase MnmA OS=Borreliella bavariensis (strain ATCC BAA-2... [more]
sp|A1R0A7|MNMA_BORT95.7e-0735.85tRNA-specific 2-thiouridylase MnmA OS=Borrelia turicatae (strain 91E135) OX=3147... [more]
sp|B2S125|MNMA_BORHD7.5e-0735.85tRNA-specific 2-thiouridylase MnmA OS=Borrelia hermsii (strain HS1 / DAH) OX=314... [more]
BLAST of MELO3C032733.2 vs. TrEMBL
Match NameE-valueIdentityDescription
tr|K7LPK6|K7LPK6_SOYBN9.1e-1844.93Uncharacterized protein OS=Glycine max OX=3847 GN=100785275 PE=4 SV=1[more]
tr|A0A1S3BNL8|A0A1S3BNL8_CUCME1.2e-1433.49tRNA-specific 2-thiouridylase MnmA OS=Cucumis melo OX=3656 GN=LOC103491516 PE=3 ... [more]
tr|A0A0A0KX16|A0A0A0KX16_CUCSA1.2e-1433.49Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G291390 PE=3 SV=1[more]
tr|A0A2I0JXK8|A0A2I0JXK8_PUNGR1.8e-1344.72Uncharacterized protein OS=Punica granatum OX=22663 GN=CRG98_019384 PE=3 SV=1[more]
tr|A0A218WLN9|A0A218WLN9_PUNGR1.8e-1344.72Uncharacterized protein OS=Punica granatum OX=22663 GN=CDL15_Pgr026592 PE=3 SV=1[more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR014729Rossmann-like_a/b/a_fold
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008033 tRNA processing
cellular_component GO:0005575 cellular_component
molecular_function GO:0016783 sulfurtransferase activity
molecular_function GO:0016740 transferase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO3C032733.2.1MELO3C032733.2.1mRNA


InterPro
Analysis Name: InterPro Annotations of melon v3.6.1
Date Performed: 2018-09-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3DG3DSA:3.40.50.620coord: 80..118
e-value: 1.2E-7
score: 33.3
NoneNo IPR availablePFAMPF03054tRNA_Me_transcoord: 82..114
e-value: 3.7E-8
score: 32.5
NoneNo IPR availablePANTHERPTHR43052FAMILY NOT NAMEDcoord: 135..181
coord: 84..114
coord: 58..87

Synteny
The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
MELO3C032733.2Cucumber (Gy14) v2cgybmedB257
MELO3C032733.2Cucumber (Gy14) v1cgymedB647
MELO3C032733.2Wild cucumber (PI 183967)cpimedB292
MELO3C032733.2Cucumber (Chinese Long) v2cumedB288
MELO3C032733.2Watermelon (97103) v1medwmB480
MELO3C032733.2Silver-seed gourdcarmedB0931
MELO3C032733.2Silver-seed gourdcarmedB1005
MELO3C032733.2Cucumber (Chinese Long) v3cucmedB296
MELO3C032733.2Watermelon (97103) v2medwmbB459
MELO3C032733.2Watermelon (97103) v2medwmbB455