MELO3C026828 (gene) Melon (DHL92) v3.5.1

NameMELO3C026828
Typegene
OrganismCucumis melo (Melon (DHL92) v3.5.1)
DescriptionAsparagine synthetase
Locationchr0 : 82284 .. 84695 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTTGATGGAATGTTCTCTTTCGTGCTGTTGGACACGCGTGACAATAGTTTCGTTGTTGCTCGTGATGCAATTGGGATTACTTCCCTTTACATTGGATGGGGACTCGATGGTAATTGAAATAATTCCGAATCTCATTTGCTTGTCAATGTGTCTCAAATTGAAGCTCCATAGACAAACGTTCTTCTAATACACTTTGATTTTGATTATGTCAGGCTCAGTTTGGATATCTTCAGAACTCAAAGGTCTGAATGATGATTGTGAACACTTTGAGACCTTTCCACCAGGTCACCTGTACTCTAGCAAGGAAGCTAAATTCAAAAGATGGTATAATCCAACTTGGTTTTCTGAGGATATTCCATCGACCCCATATGATCCTCTTGTTTTGAGACGCGCATTGGAGAACGTATGAATTAGTGTGGTGGATTTGAAGACACTTTACTAAGTTTTTAACATCTTTCGAAATTCTATTGAGTAACGAGCTTACATGCTAGCAAATTTATGTAGGCTGTGATCAAAAGGTTGATGACTGATGTTCCTTTTGGAGTTTTACTATCTGGTGGCCTTGATTCATCATTGGTTGCCTCTATCACAGCTCGTCACTTAACTGGCACAAGAGCTGCTAAGCATTGGGGTACACAACTCCATTCTTTCTGTGTTGGTCTTGAGGTTGGTTTCTTTTTCTTAGCCTTGTTGCTCCTACAATTATTGATTGTGTACATATTGAGAATCATTTCTCTTAATAACATTTGTCCTCTAATGCTCAGGGTTCACCAGATTTGAAGGCTGCTAGAGAAGTTGCTGATTTTTTGGGAACTGTCCACCATGAGTTTCATTTCACAGTTCAAGTATGTCATACTCATAACTCAACAAACAGATGTACAATCCCCACTTTCCTTGACCTACAATTTTAACATATTTATTTTCTTTTATAATAGGATGGGATTGATGCTATTGAAGATGTTATCTACCATATAGAAACATATGATGTAACTACGATCAGGGCAAGCACACCCATGTATCTTATGGCACGAAAGATTAAGTCACTAGGAGTAAAGATGGTGATTTCTGGTGAAGGCGCCGATGAGATTTTTGGTGGATACTTGTACTTCCACAAGGCTCCTAATAAGGAAGAGTTTCACCGTGAAAGTTGTCGCAAGGTATATGGTTTTATGAAGAGCACTACTTGATTTTCATAAGGTCCAAGATGATGGGAATTGCATTCATATCAGAAACCTCTTTGTTTTCCCTTTGCAGATTAAGGCACTTCACATGTATGATTGCTTAAGAGCAAACAAGGCTACTTCAGCTTGGGGCTTGGAAGTTCGAGTTCCATTTTTAGATAAGGAATTTATTAATGTTGCGATGGCCATTGATCCAGAATGGAAAATGGTAGAGAAATAAACTTCACGATATCTATCAAAATTAGCTTTTGCCCCCCCTAAATCTCTAAGTCCTTTCTCGTTTCTCATTTTATCTTTTAATCTATTTCAGATCAAACGTGATCAAGGTCGAATTGAAAAATGGGTTCTTAGGAGAGCTTTCGATGATGAGCAACAACCTTATCTTCCCAAGGTATTTGAAAGAAGAACCCCTCTAGTAGTATACAAAAACTATAACTTCAACAAGAATGTAACGCTCTGTTCTTGTCTACAGCATGTTTTGTACAGGCAAAAGGAACAATTTAGTGATGGTGTTGGATATAGTTGGATCGATGGCCTTAAAGCACATGCTGCTCAACATGTAATTTGATAAACTTCTTCGAGCCCATTTGATGATGTTTTTGTTGAAGAATGAAGTTTATAAATCTAACCTTTTGGAAGTTATGTTCATATGCTTGATGATGAATGTTCAATACATGCTTTTAGGTGACGGATAAAATGATGTTGAATGCTGAACACATCTTCCCCCAAAATACTCCAACATCAAAGGAAGCCTACTACTACAGAACCATATTTGAGAGGTTCTTCCCACAGGTAATTTACTTCCATCAACGACAGCGTTAACTGTTAACAAACATTCTTAGTAAATTCCATTAACCTTGTTTTGCACATTGTATGTCTTTTCAGAACTCAGCTCGTCTGACTGTTCCGGGAGGACCAAGTATCGCTTGCAGCACGGCAAAGGCTATCGAATGGGATGCTGCTTGGTCCAAAAATCTTGATCCTTCAGGTAGAGCTGCACTTGGAGTTCATGCTGCAGCTTATGGAGATGAGGCAAATGGTGTAAACATCGCCCCACCGAAGATAATCGATAGCATTCCGAGAATGGAGGTTGGCACACCAGGAGTAAAAATCCTTAGCTAGTATAGGATTGTAGTAGGGTTGTCGTCTTGCTTTATGATAAATCATGTCCTGTAAATTGTATGCCTTAAATGTACAAACAATATGGGAGCAATGTAGAGTGTGAAGG

mRNA sequence

ATGCTTGATGGAATGTTCTCTTTCGTGCTGTTGGACACGCGTGACAATAGTTTCGTTGTTGCTCGTGATGCAATTGGGATTACTTCCCTTTACATTGGATGGGGACTCGATGGCTCAGTTTGGATATCTTCAGAACTCAAAGGTCTGAATGATGATTGTGAACACTTTGAGACCTTTCCACCAGGTCACCTGTACTCTAGCAAGGAAGCTAAATTCAAAAGATGGTATAATCCAACTTGGTTTTCTGAGGATATTCCATCGACCCCATATGATCCTCTTGTTTTGAGACGCGCATTGGAGAACGCTGTGATCAAAAGGTTGATGACTGATGTTCCTTTTGGAGTTTTACTATCTGGTGGCCTTGATTCATCATTGGTTGCCTCTATCACAGCTCGTCACTTAACTGGCACAAGAGCTGCTAAGCATTGGGGTACACAACTCCATTCTTTCTGTGTTGGTCTTGAGGGTTCACCAGATTTGAAGGCTGCTAGAGAAGTTGCTGATTTTTTGGGAACTGTCCACCATGAGTTTCATTTCACAGTTCAAGATGGGATTGATGCTATTGAAGATGTTATCTACCATATAGAAACATATGATGTAACTACGATCAGGGCAAGCACACCCATGTATCTTATGGCACGAAAGATTAAGTCACTAGGAGTAAAGATGGTGATTTCTGGTGAAGGCGCCGATGAGATTTTTGGTGGATACTTGTACTTCCACAAGGCTCCTAATAAGGAAGAGTTTCACCGTGAAAGTTGTCGCAAGATTAAGGCACTTCACATGTATGATTGCTTAAGAGCAAACAAGGCTACTTCAGCTTGGGGCTTGGAAGTTCGAGTTCCATTTTTAGATAAGGAATTTATTAATGTTGCGATGGCCATTGATCCAGAATGGAAAATGATCAAACGTGATCAAGGTCGAATTGAAAAATGGGTTCTTAGGAGAGCTTTCGATGATGAGCAACAACCTTATCTTCCCAAGCATGTTTTGTACAGGCAAAAGGAACAATTTAGTGATGGTGTTGGATATAGTTGGATCGATGGCCTTAAAGCACATGCTGCTCAACATGTGACGGATAAAATGATGTTGAATGCTGAACACATCTTCCCCCAAAATACTCCAACATCAAAGGAAGCCTACTACTACAGAACCATATTTGAGAGGTTCTTCCCACAGAACTCAGCTCGTCTGACTGTTCCGGGAGGACCAAGTATCGCTTGCAGCACGGCAAAGGCTATCGAATGGGATGCTGCTTGGTCCAAAAATCTTGATCCTTCAGGTAGAGCTGCACTTGGAGTTCATGCTGCAGCTTATGGAGATGAGGCAAATGGTGTAAACATCGCCCCACCGAAGATAATCGATAGCATTCCGAGAATGGAGGTTGGCACACCAGGAGTAAAAATCCTTAGCTAGTATAGGATTGTAGTAGGGTTGTCGTCTTGCTTTATGATAAATCATGTCCTGTAAATTGTATGCCTTAAATGTACAAACAATATGGGAGCAATGTAGAGTGTGAAGG

Coding sequence (CDS)

ATGCTTGATGGAATGTTCTCTTTCGTGCTGTTGGACACGCGTGACAATAGTTTCGTTGTTGCTCGTGATGCAATTGGGATTACTTCCCTTTACATTGGATGGGGACTCGATGGCTCAGTTTGGATATCTTCAGAACTCAAAGGTCTGAATGATGATTGTGAACACTTTGAGACCTTTCCACCAGGTCACCTGTACTCTAGCAAGGAAGCTAAATTCAAAAGATGGTATAATCCAACTTGGTTTTCTGAGGATATTCCATCGACCCCATATGATCCTCTTGTTTTGAGACGCGCATTGGAGAACGCTGTGATCAAAAGGTTGATGACTGATGTTCCTTTTGGAGTTTTACTATCTGGTGGCCTTGATTCATCATTGGTTGCCTCTATCACAGCTCGTCACTTAACTGGCACAAGAGCTGCTAAGCATTGGGGTACACAACTCCATTCTTTCTGTGTTGGTCTTGAGGGTTCACCAGATTTGAAGGCTGCTAGAGAAGTTGCTGATTTTTTGGGAACTGTCCACCATGAGTTTCATTTCACAGTTCAAGATGGGATTGATGCTATTGAAGATGTTATCTACCATATAGAAACATATGATGTAACTACGATCAGGGCAAGCACACCCATGTATCTTATGGCACGAAAGATTAAGTCACTAGGAGTAAAGATGGTGATTTCTGGTGAAGGCGCCGATGAGATTTTTGGTGGATACTTGTACTTCCACAAGGCTCCTAATAAGGAAGAGTTTCACCGTGAAAGTTGTCGCAAGATTAAGGCACTTCACATGTATGATTGCTTAAGAGCAAACAAGGCTACTTCAGCTTGGGGCTTGGAAGTTCGAGTTCCATTTTTAGATAAGGAATTTATTAATGTTGCGATGGCCATTGATCCAGAATGGAAAATGATCAAACGTGATCAAGGTCGAATTGAAAAATGGGTTCTTAGGAGAGCTTTCGATGATGAGCAACAACCTTATCTTCCCAAGCATGTTTTGTACAGGCAAAAGGAACAATTTAGTGATGGTGTTGGATATAGTTGGATCGATGGCCTTAAAGCACATGCTGCTCAACATGTGACGGATAAAATGATGTTGAATGCTGAACACATCTTCCCCCAAAATACTCCAACATCAAAGGAAGCCTACTACTACAGAACCATATTTGAGAGGTTCTTCCCACAGAACTCAGCTCGTCTGACTGTTCCGGGAGGACCAAGTATCGCTTGCAGCACGGCAAAGGCTATCGAATGGGATGCTGCTTGGTCCAAAAATCTTGATCCTTCAGGTAGAGCTGCACTTGGAGTTCATGCTGCAGCTTATGGAGATGAGGCAAATGGTGTAAACATCGCCCCACCGAAGATAATCGATAGCATTCCGAGAATGGAGGTTGGCACACCAGGAGTAAAAATCCTTAGCTAG

Protein sequence

MLDGMFSFVLLDTRDNSFVVARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLYSSKEAKFKRWYNPTWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSLVASITARHLTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGIDAIEDVIYHIETYDVTTIRASTPMYLMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPNKEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDKEFINVAMAIDPEWKMIKRDQGRIEKWVLRRAFDDEQQPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTDKMMLNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSARLTVPGGPSIACSTAKAIEWDAAWSKNLDPSGRAALGVHAAAYGDEANGVNIAPPKIIDSIPRMEVGTPGVKILS*
BLAST of MELO3C026828 vs. Swiss-Prot
Match: ASNS1_ARATH (Asparagine synthetase [glutamine-hydrolyzing] 1 OS=Arabidopsis thaliana GN=ASN1 PE=2 SV=2)

HSP 1 Score: 809.7 bits (2090), Expect = 1.7e-233
Identity = 379/460 (82.39%), Postives = 421/460 (91.52%), Query Frame = 1

Query: 1   MLDGMFSFVLLDTRDNSFVVARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFP 60
           MLDG+FSFVLLDTRDNSF+VARDAIG+TSLYIGWGLDGSVWISSE+KGLNDDCEHFETFP
Sbjct: 116 MLDGIFSFVLLDTRDNSFMVARDAIGVTSLYIGWGLDGSVWISSEMKGLNDDCEHFETFP 175

Query: 61  PGHLYSSKEAKFKRWYNPTWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGG 120
           PGH YSSK   FK+WYNP WF+E +PSTPY+PL +RRA ENAVIKRLMTDVPFGVLLSGG
Sbjct: 176 PGHFYSSKLGGFKQWYNPPWFNESVPSTPYEPLAIRRAFENAVIKRLMTDVPFGVLLSGG 235

Query: 121 LDSSLVASITARHLTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFT 180
           LDSSLVASITARHL GT+AAK WG QLHSFCVGLEGSPDLKA +EVA++LGTVHHEFHF+
Sbjct: 236 LDSSLVASITARHLAGTKAAKQWGPQLHSFCVGLEGSPDLKAGKEVAEYLGTVHHEFHFS 295

Query: 181 VQDGIDAIEDVIYHIETYDVTTIRASTPMYLMARKIKSLGVKMVISGEGADEIFGGYLYF 240
           VQDGIDAIEDVIYH+ETYDVTTIRASTPM+LM+RKIKSLGVKMV+SGEGADEIFGGYLYF
Sbjct: 296 VQDGIDAIEDVIYHVETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGADEIFGGYLYF 355

Query: 241 HKAPNKEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDKEFINVAMAIDPEWK 300
           HKAPNK+EFH+E+CRKIKALH YDCLRANK+TSA+GLE RVPFLDK+FIN AM++DPE K
Sbjct: 356 HKAPNKKEFHQETCRKIKALHKYDCLRANKSTSAFGLEARVPFLDKDFINTAMSLDPESK 415

Query: 301 MIKRDQGRIEKWVLRRAFDDEQQPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTD 360
           MIK ++GRIEKWVLRRAFDDE++PYLPKH+LYRQKEQFSDGVGYSWIDGLK HAAQ+V D
Sbjct: 416 MIKPEEGRIEKWVLRRAFDDEERPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAAQNVND 475

Query: 361 KMMLNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSARLTVPGGPSIACSTAKAIEWDAAW 420
           KMM NA HIFP NTP +KEAYYYR IFERFFPQNSARLTVPGG ++ACSTAKA+EWDA+W
Sbjct: 476 KMMSNAGHIFPHNTPNTKEAYYYRMIFERFFPQNSARLTVPGGATVACSTAKAVEWDASW 535

Query: 421 SKNLDPSGRAALGVHAAAYGDEANGVNIAPPKIIDSIPRM 461
           S N+DPSGRAA+GVH +AY  +   + I P K ID++P M
Sbjct: 536 SNNMDPSGRAAIGVHLSAYDGKNVALTIPPLKAIDNMPMM 575

BLAST of MELO3C026828 vs. Swiss-Prot
Match: ASNS_ASPOF (Asparagine synthetase [glutamine-hydrolyzing] OS=Asparagus officinalis PE=2 SV=2)

HSP 1 Score: 807.7 bits (2085), Expect = 6.5e-233
Identity = 388/479 (81.00%), Postives = 423/479 (88.31%), Query Frame = 1

Query: 1   MLDGMFSFVLLDTRDNSFVVARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFP 60
           MLDGMFSFVLLDTR+N FV ARDA+GIT LYIGWGLDGSVW+SSE+KGLNDDCEHFE FP
Sbjct: 116 MLDGMFSFVLLDTRNNCFVAARDAVGITPLYIGWGLDGSVWLSSEMKGLNDDCEHFEVFP 175

Query: 61  PGHLYSSKEAKFKRWYNPTWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGG 120
           PG+LYSS+   F+RWYNP W++E IPS PYDPLVLR+A E+AVIKRLMTDVPFGVLLSGG
Sbjct: 176 PGNLYSSRSGSFRRWYNPQWYNETIPSAPYDPLVLRKAFEDAVIKRLMTDVPFGVLLSGG 235

Query: 121 LDSSLVASITARHLTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFT 180
           LDSSLVA++TARHL G++AA+ WGTQLHSFCVGLEGSPDLKAA+EVA++LGTVHHEFHFT
Sbjct: 236 LDSSLVAAVTARHLAGSKAAEQWGTQLHSFCVGLEGSPDLKAAKEVAEYLGTVHHEFHFT 295

Query: 181 VQDGIDAIEDVIYHIETYDVTTIRASTPMYLMARKIKSLGVKMVISGEGADEIFGGYLYF 240
           VQDGIDAIEDVI+HIETYDVTTIRASTPM+LMARKIKSLGVKMVISGEG+DEIFGGYLYF
Sbjct: 296 VQDGIDAIEDVIFHIETYDVTTIRASTPMFLMARKIKSLGVKMVISGEGSDEIFGGYLYF 355

Query: 241 HKAPNKEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDKEFINVAMAIDPEWK 300
           HKAPNKEEFH E+CRKIKALH YDCLRANKATSAWGLE RVPFLDKEF++VAM+IDPE K
Sbjct: 356 HKAPNKEEFHHETCRKIKALHQYDCLRANKATSAWGLEARVPFLDKEFMDVAMSIDPESK 415

Query: 301 MIKRDQGRIEKWVLRRAFDDEQQPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTD 360
           MIK D GRIEKWVLR+AFDDE+ PYLPKH+LYRQKEQFSDGVGYSWIDGLKAHAA+HVTD
Sbjct: 416 MIKPDLGRIEKWVLRKAFDDEENPYLPKHILYRQKEQFSDGVGYSWIDGLKAHAAKHVTD 475

Query: 361 KMMLNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSARLTVPGGPSIACSTAKAIEWDAAW 420
           +MMLNA  I+P NTPT+KEAYYYR IFERFFPQNSAR TVPGGPSIACSTAKAIEWDA W
Sbjct: 476 RMMLNAARIYPHNTPTTKEAYYYRMIFERFFPQNSARFTVPGGPSIACSTAKAIEWDARW 535

Query: 421 SKNLDPSGRAALGVHAAAY----------GDEANGVNIAPPKIIDSIPRMEVGTPGVKI 470
           S NLDPSGRAALGVH +AY          G  A  +    P+I+D      V TPGV I
Sbjct: 536 SNNLDPSGRAALGVHDSAYDPPLPSSISAGKGAAMITNKKPRIVD------VATPGVVI 588

BLAST of MELO3C026828 vs. Swiss-Prot
Match: ASNS1_LOTJA (Asparagine synthetase [glutamine-hydrolyzing] 1 OS=Lotus japonicus GN=AS1 PE=2 SV=2)

HSP 1 Score: 806.2 bits (2081), Expect = 1.9e-232
Identity = 383/471 (81.32%), Postives = 424/471 (90.02%), Query Frame = 1

Query: 1   MLDGMFSFVLLDTRDNSFVVARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFP 60
           MLDG+FSFVLLDTRDN+F+VARDAIG+TSLYIGWGLDGSVWISSE+KGLNDDCEHFE FP
Sbjct: 116 MLDGIFSFVLLDTRDNTFIVARDAIGVTSLYIGWGLDGSVWISSEMKGLNDDCEHFEVFP 175

Query: 61  PGHLYSSKEAKFKRWYNPTWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGG 120
           PGHLYSS+E  F+RWYNPTWFSE IPS PYDPL +R A E AVIKRLMTDVPFGVLLSGG
Sbjct: 176 PGHLYSSRERAFRRWYNPTWFSESIPSAPYDPLAVRHAFEKAVIKRLMTDVPFGVLLSGG 235

Query: 121 LDSSLVASITARHLTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFT 180
           LDSSLVASIT+R+L  T+AA+ WG++LHSFCVGLEGSPDLKAA+EVAD+LGTVHHEF FT
Sbjct: 236 LDSSLVASITSRYLATTKAAEQWGSKLHSFCVGLEGSPDLKAAKEVADYLGTVHHEFTFT 295

Query: 181 VQDGIDAIEDVIYHIETYDVTTIRASTPMYLMARKIKSLGVKMVISGEGADEIFGGYLYF 240
           VQDGIDAIE+VIYH+ETYDVTTIRASTPM+LM+RKIKSLGVK VISGEG+DEIFGGYLYF
Sbjct: 296 VQDGIDAIEEVIYHVETYDVTTIRASTPMFLMSRKIKSLGVKWVISGEGSDEIFGGYLYF 355

Query: 241 HKAPNKEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDKEFINVAMAIDPEWK 300
           HKAPNKEEFH E+CRKIKALH YDCLRANK+T AWGLE RVPFLDKEFINVAM IDPE+K
Sbjct: 356 HKAPNKEEFHTETCRKIKALHQYDCLRANKSTFAWGLEARVPFLDKEFINVAMNIDPEYK 415

Query: 301 MIKRDQGRIEKWVLRRAFDDEQQPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTD 360
           MIKRD+GRIEK++LRRAFDDE++PYLPKH+LYRQKEQFSDGVGYSWIDGLK HAA+HVTD
Sbjct: 416 MIKRDEGRIEKYILRRAFDDEEKPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAAKHVTD 475

Query: 361 KMMLNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSARLTVPGGPSIACSTAKAIEWDAAW 420
           KM+LNA +IF  NTP +KEAYYYR IFERFFPQNSARLTVPGGP++ACSTAKA+EWDAAW
Sbjct: 476 KMILNAGNIFRHNTPLTKEAYYYRMIFERFFPQNSARLTVPGGPTVACSTAKAVEWDAAW 535

Query: 421 SKNLDPSGRAALGVHAAAYGDEANGVNIAPPKIIDSIPRMEVGTPGVKILS 472
           S NLDPSGRAALGVH +AY D+ N +    P   + +  ME  + GV I S
Sbjct: 536 SNNLDPSGRAALGVHLSAYDDKQNNLINNKPVEFEKLIPMEAPSLGVAIHS 586

BLAST of MELO3C026828 vs. Swiss-Prot
Match: ASNS_TRIVS (Asparagine synthetase [glutamine-hydrolyzing] OS=Triphysaria versicolor GN=AS PE=2 SV=3)

HSP 1 Score: 805.8 bits (2080), Expect = 2.5e-232
Identity = 383/457 (83.81%), Postives = 416/457 (91.03%), Query Frame = 1

Query: 1   MLDGMFSFVLLDTRDNSFVVARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFP 60
           MLDGMFSFVLLD+RDN+F+ ARDA GITSLYIGWGLDGSVWISSELKGL+D+CE+FE FP
Sbjct: 116 MLDGMFSFVLLDSRDNTFIAARDAFGITSLYIGWGLDGSVWISSELKGLHDECENFEVFP 175

Query: 61  PGHLYSSKEAKFKRWYNPTWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGG 120
           PGH+YSSK   F+RWYNP WFSE IPSTPYDPLVLR A E AVIKRLMTDVPFGVLLSGG
Sbjct: 176 PGHVYSSKTEGFRRWYNPPWFSEAIPSTPYDPLVLRGAFEQAVIKRLMTDVPFGVLLSGG 235

Query: 121 LDSSLVASITARHLTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFT 180
           LDSSLVA++TARHL GT+AAK WG+QLHSFCVGLEGSPDLKA +EVAD+LGTVHHEF FT
Sbjct: 236 LDSSLVAAVTARHLAGTKAAKRWGSQLHSFCVGLEGSPDLKAGKEVADYLGTVHHEFLFT 295

Query: 181 VQDGIDAIEDVIYHIETYDVTTIRASTPMYLMARKIKSLGVKMVISGEGADEIFGGYLYF 240
           VQDGIDAIEDVIYHIETYDVTTIRASTPM+LM+RKIKSLGVKMVISGEG+DEIFGGYLYF
Sbjct: 296 VQDGIDAIEDVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVISGEGSDEIFGGYLYF 355

Query: 241 HKAPNKEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDKEFINVAMAIDPEWK 300
           HKAPNKEEFHRE+CRKIKALH YDCLRANKATSAWGLE RVPFLDKEF+N+AM+IDPE K
Sbjct: 356 HKAPNKEEFHRETCRKIKALHQYDCLRANKATSAWGLEARVPFLDKEFVNLAMSIDPEAK 415

Query: 301 MIKRDQGRIEKWVLRRAFDDEQQPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTD 360
           MIK DQGRIEKW+LR+AFDDE++PYLPKH+LYRQKEQFSDGVGYSWIDGLKAHA QHVTD
Sbjct: 416 MIKPDQGRIEKWILRKAFDDEERPYLPKHILYRQKEQFSDGVGYSWIDGLKAHAEQHVTD 475

Query: 361 KMMLNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSARLTVPGGPSIACSTAKAIEWDAAW 420
           KMMLNA HIFP NTPT+KE YYYR IFERFFPQNSA+LTVPGGPS+ACSTA A+ WDA+W
Sbjct: 476 KMMLNAGHIFPHNTPTTKEGYYYRMIFERFFPQNSAKLTVPGGPSVACSTATAVAWDASW 535

Query: 421 SKNLDPSGRAALGVHAAAYGDEANGVNIAPPKIIDSI 458
           SKNLDPSGRAA GVH  AY +     N+   K +DS+
Sbjct: 536 SKNLDPSGRAATGVHDLAYENHVPIGNLKSKK-MDSV 571

BLAST of MELO3C026828 vs. Swiss-Prot
Match: ASNS2_LOTJA (Asparagine synthetase [glutamine-hydrolyzing] 2 OS=Lotus japonicus GN=AS2 PE=2 SV=2)

HSP 1 Score: 800.4 bits (2066), Expect = 1.0e-230
Identity = 383/474 (80.80%), Postives = 423/474 (89.24%), Query Frame = 1

Query: 1   MLDGMFSFVLLDTRDNSFVVARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFP 60
           MLDG+FSFVLLDTRDNSF+VARDAIG+TSLYIG+GLDGSVWI+SELKGLNDDCEHFE FP
Sbjct: 116 MLDGIFSFVLLDTRDNSFLVARDAIGVTSLYIGYGLDGSVWIASELKGLNDDCEHFELFP 175

Query: 61  PGHLYSSKEAKFKRWYNPTWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGG 120
           PGHLYSSKE +F+RWYNP WFSE IPS PYDPL LR+A E A+IKRLMTDVPFGVLLSGG
Sbjct: 176 PGHLYSSKEKEFRRWYNPPWFSEAIPSAPYDPLALRQAFEKAIIKRLMTDVPFGVLLSGG 235

Query: 121 LDSSLVASITARHLTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFT 180
           LDSSLVAS+TAR+L  T+AAK WG++LHSFCVGLEG+PDLKAAREVAD++GTVHHEF +T
Sbjct: 236 LDSSLVASVTARYLADTKAAKQWGSKLHSFCVGLEGAPDLKAAREVADYIGTVHHEFQYT 295

Query: 181 VQDGIDAIEDVIYHIETYDVTTIRASTPMYLMARKIKSLGVKMVISGEGADEIFGGYLYF 240
           +QDGIDAIEDVIYH+ETYDVTTIRA TPM+LM+RKIKSLGVKMV+SGEG+DEIF GYLYF
Sbjct: 296 IQDGIDAIEDVIYHVETYDVTTIRAGTPMFLMSRKIKSLGVKMVLSGEGSDEIFAGYLYF 355

Query: 241 HKAPNKEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDKEFINVAMAIDPEWK 300
           HKAPNKEE H+E+C KIKALH YDCLRANK+T AWGLE RVPFLDK+FI+VAM IDPE K
Sbjct: 356 HKAPNKEELHQETCSKIKALHKYDCLRANKSTYAWGLEARVPFLDKKFIDVAMGIDPENK 415

Query: 301 MIKRDQGRIEKWVLRRAFDDEQQPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTD 360
           MIKRD+GRIEKWVLR+AFDDE+ PYLPKH+LYRQKEQFSDGVGY WIDGLK HAA+HVTD
Sbjct: 416 MIKRDEGRIEKWVLRKAFDDEENPYLPKHILYRQKEQFSDGVGYGWIDGLKDHAAKHVTD 475

Query: 361 KMMLNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSARLTVPGGPSIACSTAKAIEWDAAW 420
           KMMLNA +I+P NTP +KEAYYYR IFERFFPQNSARL+VPGG SIACST KAIEWDAAW
Sbjct: 476 KMMLNASNIYPFNTPNTKEAYYYRMIFERFFPQNSARLSVPGGASIACSTEKAIEWDAAW 535

Query: 421 SKNLDPSGRAALGVHAAAYGDEAN---GVNIAPPKIIDSIPRMEVGTPGVKILS 472
           S NLDPSGRAALGVH +AY D+ N      + P KI   IP+MEV   GV ILS
Sbjct: 536 SNNLDPSGRAALGVHDSAYDDQLNKSVSKGVEPEKI---IPKMEVSPLGVAILS 586

BLAST of MELO3C026828 vs. TrEMBL
Match: A0A061GZV4_THECC (Glutamine-dependent asparagine synthase 1 OS=Theobroma cacao GN=TCM_041592 PE=4 SV=1)

HSP 1 Score: 854.4 bits (2206), Expect = 6.7e-245
Identity = 411/471 (87.26%), Postives = 437/471 (92.78%), Query Frame = 1

Query: 1   MLDGMFSFVLLDTRDNSFVVARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFP 60
           MLDG+FSFVLLDTRDNSF+VARDAIG+TSLY GWGLDGSVWISSELKGLNDDCEHFETFP
Sbjct: 116 MLDGIFSFVLLDTRDNSFIVARDAIGVTSLYFGWGLDGSVWISSELKGLNDDCEHFETFP 175

Query: 61  PGHLYSSKEAKFKRWYNPTWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGG 120
           PGHLYSSK   F+RWYNP WFSE +PS PYDPLVLR A ENAVIKRLMTDVPFGVLLSGG
Sbjct: 176 PGHLYSSKLGGFRRWYNPPWFSEAVPSVPYDPLVLRHAFENAVIKRLMTDVPFGVLLSGG 235

Query: 121 LDSSLVASITARHLTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFT 180
           LDSSLVASITAR+L GT+AAKHWGTQLHSFC+GLEGSPDLKAAREVAD+LGTVHHEFHFT
Sbjct: 236 LDSSLVASITARYLAGTKAAKHWGTQLHSFCIGLEGSPDLKAAREVADYLGTVHHEFHFT 295

Query: 181 VQDGIDAIEDVIYHIETYDVTTIRASTPMYLMARKIKSLGVKMVISGEGADEIFGGYLYF 240
           VQDGIDAIEDVIYHIETYDVTTIRASTPM+LM+RKIKSLGVKMVISGEG+DEIFGGYLYF
Sbjct: 296 VQDGIDAIEDVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVISGEGSDEIFGGYLYF 355

Query: 241 HKAPNKEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDKEFINVAMAIDPEWK 300
           HKAPNKEEFHRE+C KIKALH YDCLRANKATSAWGLE RVPFLDKEFINVAMAIDPE K
Sbjct: 356 HKAPNKEEFHRETCHKIKALHQYDCLRANKATSAWGLEARVPFLDKEFINVAMAIDPESK 415

Query: 301 MIKRDQGRIEKWVLRRAFDDEQQPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTD 360
           +IK+DQGRIEKWVLRRAFDDE++PYLPKH+LYRQKEQFSDGVGYSWIDGLKAHAAQHVTD
Sbjct: 416 LIKKDQGRIEKWVLRRAFDDEERPYLPKHILYRQKEQFSDGVGYSWIDGLKAHAAQHVTD 475

Query: 361 KMMLNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSARLTVPGGPSIACSTAKAIEWDAAW 420
           KMMLNA +IFP NTPT+KEAYYYR IFERFFPQNSARLTVPGG S+ACSTAKA+EWDAAW
Sbjct: 476 KMMLNASYIFPHNTPTTKEAYYYRMIFERFFPQNSARLTVPGGASVACSTAKAVEWDAAW 535

Query: 421 SKNLDPSGRAALGVHAAAYGDEANGVNIAPPKIIDSIPRMEVGTPGVKILS 472
             NLDPSGRAALGVH +AY  E    N+ P K+IDSIPRM +  PGV I S
Sbjct: 536 KNNLDPSGRAALGVHLSAYDAETPLSNV-PSKVIDSIPRM-MDLPGVAIQS 584

BLAST of MELO3C026828 vs. TrEMBL
Match: A0A067LBF0_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_17398 PE=4 SV=1)

HSP 1 Score: 853.2 bits (2203), Expect = 1.5e-244
Identity = 408/474 (86.08%), Postives = 437/474 (92.19%), Query Frame = 1

Query: 1   MLDGMFSFVLLDTRDNSFVVARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFP 60
           MLDGMFSFV+LDTRDNS++VARDAIGITSLYIGWGLDGS+WI+SELKGLNDDCEHFE+FP
Sbjct: 116 MLDGMFSFVILDTRDNSYIVARDAIGITSLYIGWGLDGSIWIASELKGLNDDCEHFESFP 175

Query: 61  PGHLYSSKEAKFKRWYNPTWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGG 120
           PGHLYSSK    + WYNP WFS+ IPSTPYDPL LRRA ENAV+KRLMTDVPFGVLLSGG
Sbjct: 176 PGHLYSSKSGGLRPWYNPPWFSDVIPSTPYDPLALRRAFENAVVKRLMTDVPFGVLLSGG 235

Query: 121 LDSSLVASITARHLTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFT 180
           LDSSLVASITARHL GT+AAK WG+QLHSFCVGLEGSPDLKAA+EVAD+LGTVHHEFHFT
Sbjct: 236 LDSSLVASITARHLAGTKAAKQWGSQLHSFCVGLEGSPDLKAAKEVADYLGTVHHEFHFT 295

Query: 181 VQDGIDAIEDVIYHIETYDVTTIRASTPMYLMARKIKSLGVKMVISGEGADEIFGGYLYF 240
           VQDGIDAIEDVIYHIETYDVTTIRASTPM+LM+RKIK+LGVKMV+SGEG+DE+FGGYLYF
Sbjct: 296 VQDGIDAIEDVIYHIETYDVTTIRASTPMFLMSRKIKALGVKMVLSGEGSDEVFGGYLYF 355

Query: 241 HKAPNKEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDKEFINVAMAIDPEWK 300
           HKAPNKEEFHRE+CRKIKALH YDCLRANKATSAWGLE RVPFLDKEFINVAMAIDPEWK
Sbjct: 356 HKAPNKEEFHRETCRKIKALHQYDCLRANKATSAWGLEARVPFLDKEFINVAMAIDPEWK 415

Query: 301 MIKRDQGRIEKWVLRRAFDDEQQPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTD 360
           MIK +QGRIEKWVLRRAFDDE+QPYLPKH+LYRQKEQFSDGVGYSWIDGLKAHAAQHVTD
Sbjct: 416 MIKPEQGRIEKWVLRRAFDDEEQPYLPKHILYRQKEQFSDGVGYSWIDGLKAHAAQHVTD 475

Query: 361 KMMLNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSARLTVPGGPSIACSTAKAIEWDAAW 420
           KMM NA HIFP NTPT+KEAYYYR IFERFFPQNSARLTVPGG S+ACSTAKA+EWDA W
Sbjct: 476 KMMQNAGHIFPHNTPTTKEAYYYRMIFERFFPQNSARLTVPGGASVACSTAKAVEWDATW 535

Query: 421 SKNLDPSGRAALGVHAAAYGDEANGVN--IAPPKIIDSIPR-MEVGTPGVKILS 472
           S NLDPSGRAALGVH AAY  +A   N  +  P IIDSIPR MEV  P + ILS
Sbjct: 536 SNNLDPSGRAALGVHLAAYDPQAAAANPAMVTPNIIDSIPRMMEVTAPELTILS 589

BLAST of MELO3C026828 vs. TrEMBL
Match: G3G8K0_VITVI (Asparagine synthetase 1 OS=Vitis vinifera PE=2 SV=1)

HSP 1 Score: 852.8 bits (2202), Expect = 2.0e-244
Identity = 410/468 (87.61%), Postives = 436/468 (93.16%), Query Frame = 1

Query: 1   MLDGMFSFVLLDTRDNSFVVARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFP 60
           MLDGMFSFVLLDTRD+SF+VARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFE+FP
Sbjct: 116 MLDGMFSFVLLDTRDDSFIVARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFESFP 175

Query: 61  PGHLYSSKEAKFKRWYNPTWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGG 120
           PGH+YSSKE  FKRWYNP WFSE IPS PYDPLVLRRA ENAVIKRLMTDVPFGVLLSGG
Sbjct: 176 PGHMYSSKEGGFKRWYNPPWFSEAIPSAPYDPLVLRRAFENAVIKRLMTDVPFGVLLSGG 235

Query: 121 LDSSLVASITARHLTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFT 180
           LDSSLVASITARHL GT+AAK WG QLHSFCVGLEGSPDLKAA+EVAD+LGTVHHEFHFT
Sbjct: 236 LDSSLVASITARHLAGTKAAKQWGAQLHSFCVGLEGSPDLKAAKEVADYLGTVHHEFHFT 295

Query: 181 VQDGIDAIEDVIYHIETYDVTTIRASTPMYLMARKIKSLGVKMVISGEGADEIFGGYLYF 240
           VQDGIDAIEDVIYHIETYDVTTIRASTPM+LM+RKIKSLGVKMVISGEG+DEIFGGYLYF
Sbjct: 296 VQDGIDAIEDVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVISGEGSDEIFGGYLYF 355

Query: 241 HKAPNKEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDKEFINVAMAIDPEWK 300
           HKAPNKEEFHRE+CRKIKAL+ YDCLRANK+TSAWGLE RVPFLDKEFI VAM IDPEWK
Sbjct: 356 HKAPNKEEFHRETCRKIKALYQYDCLRANKSTSAWGLEARVPFLDKEFIKVAMDIDPEWK 415

Query: 301 MIKRDQGRIEKWVLRRAFDDEQQPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTD 360
           MIK +QGRIEKWVLRRAFDDE+QPYLPKH+LYRQKEQFSDGVGYSWIDGLKAHA+QHVTD
Sbjct: 416 MIKPEQGRIEKWVLRRAFDDEEQPYLPKHILYRQKEQFSDGVGYSWIDGLKAHASQHVTD 475

Query: 361 KMMLNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSARLTVPGGPSIACSTAKAIEWDAAW 420
           KMMLNA HIFP NTPT+KEAYYYR IFERFFPQNSARLTVPGG S+ACSTAKA+EWD+AW
Sbjct: 476 KMMLNASHIFPHNTPTTKEAYYYRMIFERFFPQNSARLTVPGGASVACSTAKAVEWDSAW 535

Query: 421 SKNLDPSGRAALGVHAAAYGDEANGVNIA--PPKIIDSIPR-MEVGTP 466
           S NLDPSGRAALGVH +AY  +   V+ A  P KIID++PR MEV  P
Sbjct: 536 SNNLDPSGRAALGVHLSAYDQKLTTVSAANVPTKIIDNMPRIMEVTAP 583

BLAST of MELO3C026828 vs. TrEMBL
Match: D7SJV6_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_06s0004g06830 PE=4 SV=1)

HSP 1 Score: 852.8 bits (2202), Expect = 2.0e-244
Identity = 410/468 (87.61%), Postives = 436/468 (93.16%), Query Frame = 1

Query: 1   MLDGMFSFVLLDTRDNSFVVARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFP 60
           MLDGMFSFVLLDTRD+SF+VARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFE+FP
Sbjct: 116 MLDGMFSFVLLDTRDDSFIVARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFESFP 175

Query: 61  PGHLYSSKEAKFKRWYNPTWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGG 120
           PGH+YSSKE  FKRWYNP WFSE IPS PYDPLVLRRA ENAVIKRLMTDVPFGVLLSGG
Sbjct: 176 PGHMYSSKEGGFKRWYNPPWFSEAIPSAPYDPLVLRRAFENAVIKRLMTDVPFGVLLSGG 235

Query: 121 LDSSLVASITARHLTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFT 180
           LDSSLVASITARHL GT+AAK WG QLHSFCVGLEGSPDLKAA+EVAD+LGTVHHEFHFT
Sbjct: 236 LDSSLVASITARHLAGTKAAKQWGAQLHSFCVGLEGSPDLKAAKEVADYLGTVHHEFHFT 295

Query: 181 VQDGIDAIEDVIYHIETYDVTTIRASTPMYLMARKIKSLGVKMVISGEGADEIFGGYLYF 240
           VQDGIDAIEDVIYHIETYDVTTIRASTPM+LM+RKIKSLGVKMVISGEG+DEIFGGYLYF
Sbjct: 296 VQDGIDAIEDVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVISGEGSDEIFGGYLYF 355

Query: 241 HKAPNKEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDKEFINVAMAIDPEWK 300
           HKAPNKEEFHRE+CRKIKAL+ YDCLRANK+TSAWGLE RVPFLDKEFI VAM IDPEWK
Sbjct: 356 HKAPNKEEFHRETCRKIKALYQYDCLRANKSTSAWGLEARVPFLDKEFIKVAMDIDPEWK 415

Query: 301 MIKRDQGRIEKWVLRRAFDDEQQPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTD 360
           MIK +QGRIEKWVLRRAFDDE+QPYLPKH+LYRQKEQFSDGVGYSWIDGLKAHA+QHVTD
Sbjct: 416 MIKPEQGRIEKWVLRRAFDDEEQPYLPKHILYRQKEQFSDGVGYSWIDGLKAHASQHVTD 475

Query: 361 KMMLNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSARLTVPGGPSIACSTAKAIEWDAAW 420
           KMMLNA HIFP NTPT+KEAYYYR IFERFFPQNSARLTVPGG S+ACSTAKA+EWD+AW
Sbjct: 476 KMMLNASHIFPHNTPTTKEAYYYRMIFERFFPQNSARLTVPGGASVACSTAKAVEWDSAW 535

Query: 421 SKNLDPSGRAALGVHAAAYGDEANGVNIA--PPKIIDSIPR-MEVGTP 466
           S NLDPSGRAALGVH +AY  +   V+ A  P KIID++PR MEV  P
Sbjct: 536 SNNLDPSGRAALGVHLSAYDQKLTTVSAANVPTKIIDNMPRIMEVTAP 583

BLAST of MELO3C026828 vs. TrEMBL
Match: B9SL40_RICCO (Asparagine synthetase, putative OS=Ricinus communis GN=RCOM_0479760 PE=4 SV=1)

HSP 1 Score: 847.8 bits (2189), Expect = 6.3e-243
Identity = 410/476 (86.13%), Postives = 434/476 (91.18%), Query Frame = 1

Query: 1   MLDGMFSFVLLDTRDNSFVVARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFP 60
           MLDGMFSFVLLDTRDNSF+VARDAIGITSLYIGWGLDGS+WISSELKGLNDDCEHFE FP
Sbjct: 116 MLDGMFSFVLLDTRDNSFIVARDAIGITSLYIGWGLDGSIWISSELKGLNDDCEHFECFP 175

Query: 61  PGHLYSSKEAKFKRWYNPTWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGG 120
           PGHLYSSK    +RWYNP WFSE IPSTPYDPL LRRA ENAVIKRLMTDVPFGVLLSGG
Sbjct: 176 PGHLYSSKSGGLRRWYNPPWFSEAIPSTPYDPLALRRAFENAVIKRLMTDVPFGVLLSGG 235

Query: 121 LDSSLVASITARHLTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFT 180
           LDSSLVASITARHL  T+AAK WG QLHSFCVGLEGSPDLKAA+EVAD+LGTVHHEFHFT
Sbjct: 236 LDSSLVASITARHLADTKAAKQWGAQLHSFCVGLEGSPDLKAAKEVADYLGTVHHEFHFT 295

Query: 181 VQDGIDAIEDVIYHIETYDVTTIRASTPMYLMARKIKSLGVKMVISGEGADEIFGGYLYF 240
           VQDGIDAIEDVIYHIETYDVTTIRASTPM+LMARKIK+LGVKMVISGEG+DEIFGGYLYF
Sbjct: 296 VQDGIDAIEDVIYHIETYDVTTIRASTPMFLMARKIKALGVKMVISGEGSDEIFGGYLYF 355

Query: 241 HKAPNKEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDKEFINVAMAIDPEWK 300
           HKAPNKEEFHRE+C KIKALH YDCLRANKATSAWGLE R+PFLDKEFINVAMAI PE K
Sbjct: 356 HKAPNKEEFHRETCHKIKALHQYDCLRANKATSAWGLEARIPFLDKEFINVAMAIGPESK 415

Query: 301 MIKRDQGRIEKWVLRRAFDDEQQPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTD 360
           MIK ++GRIEKWVLRRAFDDE+ PYLPKH+LYRQKEQFSDGVGYSWIDGLKAHAA+HVTD
Sbjct: 416 MIKPEEGRIEKWVLRRAFDDEEHPYLPKHILYRQKEQFSDGVGYSWIDGLKAHAAEHVTD 475

Query: 361 KMMLNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSARLTVPGGPSIACSTAKAIEWDAAW 420
           KM+ NAEHIF  NTPT+KEAYYYR IFERFFPQNSARLTVPGG SIACSTAKA+EWDAAW
Sbjct: 476 KMIQNAEHIFTHNTPTTKEAYYYRMIFERFFPQNSARLTVPGGASIACSTAKAVEWDAAW 535

Query: 421 SKNLDPSGRAALGVHAAAYGDEA----NGVNIAPPKIIDSIPR-MEVGTPGVKILS 472
           S NLDPSGRAALGVH +AYG +A        + PPKIIDS+PR MEV  PG+ I S
Sbjct: 536 SDNLDPSGRAALGVHLSAYGKQAAAAVASAGMVPPKIIDSMPRMMEVSPPGLTISS 591

BLAST of MELO3C026828 vs. TAIR10
Match: AT3G47340.1 (AT3G47340.1 glutamine-dependent asparagine synthase 1)

HSP 1 Score: 809.7 bits (2090), Expect = 9.6e-235
Identity = 379/460 (82.39%), Postives = 421/460 (91.52%), Query Frame = 1

Query: 1   MLDGMFSFVLLDTRDNSFVVARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFP 60
           MLDG+FSFVLLDTRDNSF+VARDAIG+TSLYIGWGLDGSVWISSE+KGLNDDCEHFETFP
Sbjct: 116 MLDGIFSFVLLDTRDNSFMVARDAIGVTSLYIGWGLDGSVWISSEMKGLNDDCEHFETFP 175

Query: 61  PGHLYSSKEAKFKRWYNPTWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGG 120
           PGH YSSK   FK+WYNP WF+E +PSTPY+PL +RRA ENAVIKRLMTDVPFGVLLSGG
Sbjct: 176 PGHFYSSKLGGFKQWYNPPWFNESVPSTPYEPLAIRRAFENAVIKRLMTDVPFGVLLSGG 235

Query: 121 LDSSLVASITARHLTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFT 180
           LDSSLVASITARHL GT+AAK WG QLHSFCVGLEGSPDLKA +EVA++LGTVHHEFHF+
Sbjct: 236 LDSSLVASITARHLAGTKAAKQWGPQLHSFCVGLEGSPDLKAGKEVAEYLGTVHHEFHFS 295

Query: 181 VQDGIDAIEDVIYHIETYDVTTIRASTPMYLMARKIKSLGVKMVISGEGADEIFGGYLYF 240
           VQDGIDAIEDVIYH+ETYDVTTIRASTPM+LM+RKIKSLGVKMV+SGEGADEIFGGYLYF
Sbjct: 296 VQDGIDAIEDVIYHVETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGADEIFGGYLYF 355

Query: 241 HKAPNKEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDKEFINVAMAIDPEWK 300
           HKAPNK+EFH+E+CRKIKALH YDCLRANK+TSA+GLE RVPFLDK+FIN AM++DPE K
Sbjct: 356 HKAPNKKEFHQETCRKIKALHKYDCLRANKSTSAFGLEARVPFLDKDFINTAMSLDPESK 415

Query: 301 MIKRDQGRIEKWVLRRAFDDEQQPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTD 360
           MIK ++GRIEKWVLRRAFDDE++PYLPKH+LYRQKEQFSDGVGYSWIDGLK HAAQ+V D
Sbjct: 416 MIKPEEGRIEKWVLRRAFDDEERPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAAQNVND 475

Query: 361 KMMLNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSARLTVPGGPSIACSTAKAIEWDAAW 420
           KMM NA HIFP NTP +KEAYYYR IFERFFPQNSARLTVPGG ++ACSTAKA+EWDA+W
Sbjct: 476 KMMSNAGHIFPHNTPNTKEAYYYRMIFERFFPQNSARLTVPGGATVACSTAKAVEWDASW 535

Query: 421 SKNLDPSGRAALGVHAAAYGDEANGVNIAPPKIIDSIPRM 461
           S N+DPSGRAA+GVH +AY  +   + I P K ID++P M
Sbjct: 536 SNNMDPSGRAAIGVHLSAYDGKNVALTIPPLKAIDNMPMM 575

BLAST of MELO3C026828 vs. TAIR10
Match: AT5G10240.1 (AT5G10240.1 asparagine synthetase 3)

HSP 1 Score: 762.7 bits (1968), Expect = 1.3e-220
Identity = 350/442 (79.19%), Postives = 397/442 (89.82%), Query Frame = 1

Query: 1   MLDGMFSFVLLDTRDNSFVVARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFP 60
           MLDGMF+FVLLDTRD SF+ ARDAIGIT LYIGWGLDGSVW +SE+K L+DDCE F  FP
Sbjct: 116 MLDGMFAFVLLDTRDKSFIAARDAIGITPLYIGWGLDGSVWFASEMKALSDDCEQFMCFP 175

Query: 61  PGHLYSSKEAKFKRWYNPTWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGG 120
           PGH+YSSK+   +RWYNP WFSE +PSTPYDPLV+R   E AVIKRLMTDVPFGVLLSGG
Sbjct: 176 PGHIYSSKQGGLRRWYNPPWFSEVVPSTPYDPLVVRNTFEKAVIKRLMTDVPFGVLLSGG 235

Query: 121 LDSSLVASITARHLTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFT 180
           LDSSLVAS+  RHL  + AA  WG++LH+FC+GL+GSPDLKA REVAD+LGT HHE HFT
Sbjct: 236 LDSSLVASVALRHLEKSEAACQWGSKLHTFCIGLKGSPDLKAGREVADYLGTRHHELHFT 295

Query: 181 VQDGIDAIEDVIYHIETYDVTTIRASTPMYLMARKIKSLGVKMVISGEGADEIFGGYLYF 240
           VQDGIDAIE+VIYH+ETYDVTTIRASTPM+LM+RKIKSLGVKMV+SGEG+DEIFGGYLYF
Sbjct: 296 VQDGIDAIEEVIYHVETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYF 355

Query: 241 HKAPNKEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDKEFINVAMAIDPEWK 300
           HKAPNK+EFH E+CRKIKALH YDCLRANK+TSAWG+E RVPFLDKEFINVAM+IDPEWK
Sbjct: 356 HKAPNKKEFHEETCRKIKALHQYDCLRANKSTSAWGVEARVPFLDKEFINVAMSIDPEWK 415

Query: 301 MIKRDQGRIEKWVLRRAFDDEQQPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTD 360
           MI+ D GRIEKWVLR AFDDE+ PYLPKH+LYRQKEQFSDGVGYSWIDGLK HA +HV++
Sbjct: 416 MIRPDLGRIEKWVLRNAFDDEKNPYLPKHILYRQKEQFSDGVGYSWIDGLKDHANKHVSE 475

Query: 361 KMMLNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSARLTVPGGPSIACSTAKAIEWDAAW 420
            M++NA  +FP NTP +KEAYYYRTIFE+FFP+++AR TVPGGPS+ACSTAKA+EWDAAW
Sbjct: 476 TMLMNASFVFPDNTPLTKEAYYYRTIFEKFFPKSAARATVPGGPSVACSTAKAVEWDAAW 535

Query: 421 SKNLDPSGRAALGVHAAAYGDE 443
           S+NLDPSGRAALGVH +AYG++
Sbjct: 536 SQNLDPSGRAALGVHVSAYGED 557

BLAST of MELO3C026828 vs. TAIR10
Match: AT5G65010.2 (AT5G65010.2 asparagine synthetase 2)

HSP 1 Score: 762.3 bits (1967), Expect = 1.8e-220
Identity = 356/461 (77.22%), Postives = 405/461 (87.85%), Query Frame = 1

Query: 1   MLDGMFSFVLLDTRDNSFVVARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFP 60
           MLDGMF+FVLLDTRD SF+ ARDAIGIT LYIGWGLDGSVW +SE+K L+DDCE F +FP
Sbjct: 116 MLDGMFAFVLLDTRDKSFIAARDAIGITPLYIGWGLDGSVWFASEMKALSDDCEQFMSFP 175

Query: 61  PGHLYSSKEAKFKRWYNPTWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGG 120
           PGH+YSSK+   +RWYNP W++E +PSTPYDPLVLR A E AVIKRLMTDVPFGVLLSGG
Sbjct: 176 PGHIYSSKQGGLRRWYNPPWYNEQVPSTPYDPLVLRNAFEKAVIKRLMTDVPFGVLLSGG 235

Query: 121 LDSSLVASITARHLTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFT 180
           LDSSLVA++  RHL  + AA+ WG+QLH+FC+GL+GSPDLKA REVAD+LGT HHEF FT
Sbjct: 236 LDSSLVAAVALRHLEKSEAARQWGSQLHTFCIGLQGSPDLKAGREVADYLGTRHHEFQFT 295

Query: 181 VQDGIDAIEDVIYHIETYDVTTIRASTPMYLMARKIKSLGVKMVISGEGADEIFGGYLYF 240
           VQDGIDAIE+VIYHIETYDVTTIRASTPM+LM+RKIKSLGVKMV+SGEG+DEI GGYLYF
Sbjct: 296 VQDGIDAIEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEILGGYLYF 355

Query: 241 HKAPNKEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDKEFINVAMAIDPEWK 300
           HKAPNK+EFH E+CRKIKALH +DCLRANK+TSAWG+E RVPFLDKEF+NVAM+IDPEWK
Sbjct: 356 HKAPNKKEFHEETCRKIKALHQFDCLRANKSTSAWGVEARVPFLDKEFLNVAMSIDPEWK 415

Query: 301 MIKRDQGRIEKWVLRRAFDDEQQPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTD 360
           +IK D GRIEKWVLR AFDDE++PYLPKH+LYRQKEQFSDGVGYSWIDGLK HA +HV+D
Sbjct: 416 LIKPDLGRIEKWVLRNAFDDEERPYLPKHILYRQKEQFSDGVGYSWIDGLKDHANKHVSD 475

Query: 361 KMMLNAEHIFPQNTPTSKEAYYYRTIFERFFP-QNSARLTVPGGPSIACSTAKAIEWDAA 420
            M+ NA  +FP NTP +KEAYYYRTIFE+FFP Q++AR TVPGGPSIACSTAKA+EWDA 
Sbjct: 476 TMLSNASFVFPDNTPLTKEAYYYRTIFEKFFPKQSAARATVPGGPSIACSTAKAVEWDAT 535

Query: 421 WSKNLDPSGRAALGVHAAAY-GDEANGVNIAPPKIIDSIPR 460
           WSKNLDPSGRAALGVH AAY  D+A     A   ++D +P+
Sbjct: 536 WSKNLDPSGRAALGVHVAAYEEDKAAAAAKAGSDLVDPLPK 576

BLAST of MELO3C026828 vs. NCBI nr
Match: gi|778715252|ref|XP_004144212.2| (PREDICTED: LOW QUALITY PROTEIN: asparagine synthetase [glutamine-hydrolyzing])

HSP 1 Score: 909.8 bits (2350), Expect = 1.9e-261
Identity = 442/472 (93.64%), Postives = 457/472 (96.82%), Query Frame = 1

Query: 1   MLDGMFSFVLLDTRDNSFVVARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFP 60
           MLDGMFSFVLLDTRDNSF+ ARDAIG+TSLYIGWG DGSVWISSELKGLNDDCEHFETFP
Sbjct: 116 MLDGMFSFVLLDTRDNSFIAARDAIGVTSLYIGWGRDGSVWISSELKGLNDDCEHFETFP 175

Query: 61  PGHLYSSKEAKFKRWYNPTWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGG 120
           PGHLYSSKEA FKRWYNP WFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGG
Sbjct: 176 PGHLYSSKEAGFKRWYNPIWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGG 235

Query: 121 LDSSLVASITARHLTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFT 180
           LDSSLVASITAR+LTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFT
Sbjct: 236 LDSSLVASITARYLTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFT 295

Query: 181 VQDGIDAIEDVIYHIETYDVTTIRASTPMYLMARKIKSLGVKMVISGEGADEIFGGYLYF 240
           VQDGIDAIEDVIYHIETYDVTTIRASTPM+LMARKIKSLGVKMV+SGEGADEIFGGYLYF
Sbjct: 296 VQDGIDAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVVSGEGADEIFGGYLYF 355

Query: 241 HKAPNKEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDKEFINVAMAIDPEWK 300
           HKAPNKEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDK+FI+VAMAIDPEWK
Sbjct: 356 HKAPNKEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDKKFIDVAMAIDPEWK 415

Query: 301 MIKRDQGRIEKWVLRRAFDDEQQPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTD 360
           MIK +QGRIEKWVLRRAFDDEQQPYLPK+VLYRQKEQFSDGVGYSWIDGLKAHA QHVTD
Sbjct: 416 MIKHNQGRIEKWVLRRAFDDEQQPYLPKNVLYRQKEQFSDGVGYSWIDGLKAHADQHVTD 475

Query: 361 KMMLNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSARLTVPGGPSIACSTAKAIEWDAAW 420
           KM+LNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSA LTVPGGPSIACSTAKA+EWDAAW
Sbjct: 476 KMILNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSACLTVPGGPSIACSTAKAVEWDAAW 535

Query: 421 SKNLDPSGRAALGVHAAAYGDEANGVN-IAPPKIIDSIPRMEVGTPGVKILS 472
           S NL PS RAALGVHA+ YGDEANG+N I PPKI+D+IPRMEV T GVKILS
Sbjct: 536 SXNLGPS-RAALGVHASTYGDEANGLNDIVPPKIVDTIPRMEVSTAGVKILS 586

BLAST of MELO3C026828 vs. NCBI nr
Match: gi|590587838|ref|XP_007016070.1| (Glutamine-dependent asparagine synthase 1 [Theobroma cacao])

HSP 1 Score: 854.4 bits (2206), Expect = 9.7e-245
Identity = 411/471 (87.26%), Postives = 437/471 (92.78%), Query Frame = 1

Query: 1   MLDGMFSFVLLDTRDNSFVVARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFP 60
           MLDG+FSFVLLDTRDNSF+VARDAIG+TSLY GWGLDGSVWISSELKGLNDDCEHFETFP
Sbjct: 116 MLDGIFSFVLLDTRDNSFIVARDAIGVTSLYFGWGLDGSVWISSELKGLNDDCEHFETFP 175

Query: 61  PGHLYSSKEAKFKRWYNPTWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGG 120
           PGHLYSSK   F+RWYNP WFSE +PS PYDPLVLR A ENAVIKRLMTDVPFGVLLSGG
Sbjct: 176 PGHLYSSKLGGFRRWYNPPWFSEAVPSVPYDPLVLRHAFENAVIKRLMTDVPFGVLLSGG 235

Query: 121 LDSSLVASITARHLTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFT 180
           LDSSLVASITAR+L GT+AAKHWGTQLHSFC+GLEGSPDLKAAREVAD+LGTVHHEFHFT
Sbjct: 236 LDSSLVASITARYLAGTKAAKHWGTQLHSFCIGLEGSPDLKAAREVADYLGTVHHEFHFT 295

Query: 181 VQDGIDAIEDVIYHIETYDVTTIRASTPMYLMARKIKSLGVKMVISGEGADEIFGGYLYF 240
           VQDGIDAIEDVIYHIETYDVTTIRASTPM+LM+RKIKSLGVKMVISGEG+DEIFGGYLYF
Sbjct: 296 VQDGIDAIEDVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVISGEGSDEIFGGYLYF 355

Query: 241 HKAPNKEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDKEFINVAMAIDPEWK 300
           HKAPNKEEFHRE+C KIKALH YDCLRANKATSAWGLE RVPFLDKEFINVAMAIDPE K
Sbjct: 356 HKAPNKEEFHRETCHKIKALHQYDCLRANKATSAWGLEARVPFLDKEFINVAMAIDPESK 415

Query: 301 MIKRDQGRIEKWVLRRAFDDEQQPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTD 360
           +IK+DQGRIEKWVLRRAFDDE++PYLPKH+LYRQKEQFSDGVGYSWIDGLKAHAAQHVTD
Sbjct: 416 LIKKDQGRIEKWVLRRAFDDEERPYLPKHILYRQKEQFSDGVGYSWIDGLKAHAAQHVTD 475

Query: 361 KMMLNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSARLTVPGGPSIACSTAKAIEWDAAW 420
           KMMLNA +IFP NTPT+KEAYYYR IFERFFPQNSARLTVPGG S+ACSTAKA+EWDAAW
Sbjct: 476 KMMLNASYIFPHNTPTTKEAYYYRMIFERFFPQNSARLTVPGGASVACSTAKAVEWDAAW 535

Query: 421 SKNLDPSGRAALGVHAAAYGDEANGVNIAPPKIIDSIPRMEVGTPGVKILS 472
             NLDPSGRAALGVH +AY  E    N+ P K+IDSIPRM +  PGV I S
Sbjct: 536 KNNLDPSGRAALGVHLSAYDAETPLSNV-PSKVIDSIPRM-MDLPGVAIQS 584

BLAST of MELO3C026828 vs. NCBI nr
Match: gi|802541023|ref|XP_012079122.1| (PREDICTED: asparagine synthetase [glutamine-hydrolyzing])

HSP 1 Score: 853.2 bits (2203), Expect = 2.1e-244
Identity = 408/474 (86.08%), Postives = 437/474 (92.19%), Query Frame = 1

Query: 1   MLDGMFSFVLLDTRDNSFVVARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFP 60
           MLDGMFSFV+LDTRDNS++VARDAIGITSLYIGWGLDGS+WI+SELKGLNDDCEHFE+FP
Sbjct: 116 MLDGMFSFVILDTRDNSYIVARDAIGITSLYIGWGLDGSIWIASELKGLNDDCEHFESFP 175

Query: 61  PGHLYSSKEAKFKRWYNPTWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGG 120
           PGHLYSSK    + WYNP WFS+ IPSTPYDPL LRRA ENAV+KRLMTDVPFGVLLSGG
Sbjct: 176 PGHLYSSKSGGLRPWYNPPWFSDVIPSTPYDPLALRRAFENAVVKRLMTDVPFGVLLSGG 235

Query: 121 LDSSLVASITARHLTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFT 180
           LDSSLVASITARHL GT+AAK WG+QLHSFCVGLEGSPDLKAA+EVAD+LGTVHHEFHFT
Sbjct: 236 LDSSLVASITARHLAGTKAAKQWGSQLHSFCVGLEGSPDLKAAKEVADYLGTVHHEFHFT 295

Query: 181 VQDGIDAIEDVIYHIETYDVTTIRASTPMYLMARKIKSLGVKMVISGEGADEIFGGYLYF 240
           VQDGIDAIEDVIYHIETYDVTTIRASTPM+LM+RKIK+LGVKMV+SGEG+DE+FGGYLYF
Sbjct: 296 VQDGIDAIEDVIYHIETYDVTTIRASTPMFLMSRKIKALGVKMVLSGEGSDEVFGGYLYF 355

Query: 241 HKAPNKEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDKEFINVAMAIDPEWK 300
           HKAPNKEEFHRE+CRKIKALH YDCLRANKATSAWGLE RVPFLDKEFINVAMAIDPEWK
Sbjct: 356 HKAPNKEEFHRETCRKIKALHQYDCLRANKATSAWGLEARVPFLDKEFINVAMAIDPEWK 415

Query: 301 MIKRDQGRIEKWVLRRAFDDEQQPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTD 360
           MIK +QGRIEKWVLRRAFDDE+QPYLPKH+LYRQKEQFSDGVGYSWIDGLKAHAAQHVTD
Sbjct: 416 MIKPEQGRIEKWVLRRAFDDEEQPYLPKHILYRQKEQFSDGVGYSWIDGLKAHAAQHVTD 475

Query: 361 KMMLNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSARLTVPGGPSIACSTAKAIEWDAAW 420
           KMM NA HIFP NTPT+KEAYYYR IFERFFPQNSARLTVPGG S+ACSTAKA+EWDA W
Sbjct: 476 KMMQNAGHIFPHNTPTTKEAYYYRMIFERFFPQNSARLTVPGGASVACSTAKAVEWDATW 535

Query: 421 SKNLDPSGRAALGVHAAAYGDEANGVN--IAPPKIIDSIPR-MEVGTPGVKILS 472
           S NLDPSGRAALGVH AAY  +A   N  +  P IIDSIPR MEV  P + ILS
Sbjct: 536 SNNLDPSGRAALGVHLAAYDPQAAAANPAMVTPNIIDSIPRMMEVTAPELTILS 589

BLAST of MELO3C026828 vs. NCBI nr
Match: gi|1009115914|ref|XP_015874493.1| (PREDICTED: asparagine synthetase [glutamine-hydrolyzing])

HSP 1 Score: 853.2 bits (2203), Expect = 2.1e-244
Identity = 404/471 (85.77%), Postives = 436/471 (92.57%), Query Frame = 1

Query: 1   MLDGMFSFVLLDTRDNSFVVARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFP 60
           MLDGMFSFVLLDTRDNSF+VARDAIGITSLYIGWGLDGS+WISSELKGLNDDCEHFE FP
Sbjct: 94  MLDGMFSFVLLDTRDNSFIVARDAIGITSLYIGWGLDGSIWISSELKGLNDDCEHFEAFP 153

Query: 61  PGHLYSSKEAKFKRWYNPTWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGG 120
           PGHLYSSK+   +RWYNP WF+E IPS PYDPLVLR+A ENAVIKRLMTDVPFGVLLSGG
Sbjct: 154 PGHLYSSKQGGMRRWYNPPWFNEAIPSAPYDPLVLRKAFENAVIKRLMTDVPFGVLLSGG 213

Query: 121 LDSSLVASITARHLTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFT 180
           LDSSLVASITARHL GT+AAK WG QLHSFCVGLEGSPDLKAA+EVAD+LGTVHHEFHFT
Sbjct: 214 LDSSLVASITARHLAGTKAAKQWGAQLHSFCVGLEGSPDLKAAKEVADYLGTVHHEFHFT 273

Query: 181 VQDGIDAIEDVIYHIETYDVTTIRASTPMYLMARKIKSLGVKMVISGEGADEIFGGYLYF 240
           VQDGIDAIEDVIYHIETYDVTTIRASTPM+LM+RKIKSLGVKMVISGEG+DEIFGGYLYF
Sbjct: 274 VQDGIDAIEDVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVISGEGSDEIFGGYLYF 333

Query: 241 HKAPNKEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDKEFINVAMAIDPEWK 300
           HKAPNKEEFHRE+CRKIKALHMYDC RANK+TSAWGLE RVPFLDKEFI VAM IDPE+K
Sbjct: 334 HKAPNKEEFHRETCRKIKALHMYDCQRANKSTSAWGLEARVPFLDKEFIRVAMDIDPEFK 393

Query: 301 MIKRDQGRIEKWVLRRAFDDEQQPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTD 360
           MIKRD+GRIEKW+LRRAFDDE+ PYLPKH+LYRQKEQFSDGVGYSWIDGLKAHA QHV D
Sbjct: 394 MIKRDEGRIEKWILRRAFDDEKHPYLPKHILYRQKEQFSDGVGYSWIDGLKAHADQHVND 453

Query: 361 KMMLNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSARLTVPGGPSIACSTAKAIEWDAAW 420
           KMMLNA HIFP NTPT+KEAYYYR IFERFFPQNSARLTVPGG S+ACSTAKA+EWDAAW
Sbjct: 454 KMMLNAAHIFPHNTPTTKEAYYYRMIFERFFPQNSARLTVPGGASVACSTAKAVEWDAAW 513

Query: 421 SKNLDPSGRAALGVHAAAYGDEANGVNIAPPKIIDSIPRMEVGTPGVKILS 472
           S NLDPSGRAALGVH +AY ++A   N   P+II+++P++ V  PGV+I S
Sbjct: 514 SNNLDPSGRAALGVHVSAYENQAASANNLQPEIINNVPQITVSAPGVEIRS 564

BLAST of MELO3C026828 vs. NCBI nr
Match: gi|297745876|emb|CBI15932.3| (unnamed protein product [Vitis vinifera])

HSP 1 Score: 852.8 bits (2202), Expect = 2.8e-244
Identity = 410/468 (87.61%), Postives = 436/468 (93.16%), Query Frame = 1

Query: 1   MLDGMFSFVLLDTRDNSFVVARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFP 60
           MLDGMFSFVLLDTRD+SF+VARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFE+FP
Sbjct: 116 MLDGMFSFVLLDTRDDSFIVARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFESFP 175

Query: 61  PGHLYSSKEAKFKRWYNPTWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGG 120
           PGH+YSSKE  FKRWYNP WFSE IPS PYDPLVLRRA ENAVIKRLMTDVPFGVLLSGG
Sbjct: 176 PGHMYSSKEGGFKRWYNPPWFSEAIPSAPYDPLVLRRAFENAVIKRLMTDVPFGVLLSGG 235

Query: 121 LDSSLVASITARHLTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFT 180
           LDSSLVASITARHL GT+AAK WG QLHSFCVGLEGSPDLKAA+EVAD+LGTVHHEFHFT
Sbjct: 236 LDSSLVASITARHLAGTKAAKQWGAQLHSFCVGLEGSPDLKAAKEVADYLGTVHHEFHFT 295

Query: 181 VQDGIDAIEDVIYHIETYDVTTIRASTPMYLMARKIKSLGVKMVISGEGADEIFGGYLYF 240
           VQDGIDAIEDVIYHIETYDVTTIRASTPM+LM+RKIKSLGVKMVISGEG+DEIFGGYLYF
Sbjct: 296 VQDGIDAIEDVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVISGEGSDEIFGGYLYF 355

Query: 241 HKAPNKEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDKEFINVAMAIDPEWK 300
           HKAPNKEEFHRE+CRKIKAL+ YDCLRANK+TSAWGLE RVPFLDKEFI VAM IDPEWK
Sbjct: 356 HKAPNKEEFHRETCRKIKALYQYDCLRANKSTSAWGLEARVPFLDKEFIKVAMDIDPEWK 415

Query: 301 MIKRDQGRIEKWVLRRAFDDEQQPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTD 360
           MIK +QGRIEKWVLRRAFDDE+QPYLPKH+LYRQKEQFSDGVGYSWIDGLKAHA+QHVTD
Sbjct: 416 MIKPEQGRIEKWVLRRAFDDEEQPYLPKHILYRQKEQFSDGVGYSWIDGLKAHASQHVTD 475

Query: 361 KMMLNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSARLTVPGGPSIACSTAKAIEWDAAW 420
           KMMLNA HIFP NTPT+KEAYYYR IFERFFPQNSARLTVPGG S+ACSTAKA+EWD+AW
Sbjct: 476 KMMLNASHIFPHNTPTTKEAYYYRMIFERFFPQNSARLTVPGGASVACSTAKAVEWDSAW 535

Query: 421 SKNLDPSGRAALGVHAAAYGDEANGVNIA--PPKIIDSIPR-MEVGTP 466
           S NLDPSGRAALGVH +AY  +   V+ A  P KIID++PR MEV  P
Sbjct: 536 SNNLDPSGRAALGVHLSAYDQKLTTVSAANVPTKIIDNMPRIMEVTAP 583

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
ASNS1_ARATH1.7e-23382.39Asparagine synthetase [glutamine-hydrolyzing] 1 OS=Arabidopsis thaliana GN=ASN1 ... [more]
ASNS_ASPOF6.5e-23381.00Asparagine synthetase [glutamine-hydrolyzing] OS=Asparagus officinalis PE=2 SV=2[more]
ASNS1_LOTJA1.9e-23281.32Asparagine synthetase [glutamine-hydrolyzing] 1 OS=Lotus japonicus GN=AS1 PE=2 S... [more]
ASNS_TRIVS2.5e-23283.81Asparagine synthetase [glutamine-hydrolyzing] OS=Triphysaria versicolor GN=AS PE... [more]
ASNS2_LOTJA1.0e-23080.80Asparagine synthetase [glutamine-hydrolyzing] 2 OS=Lotus japonicus GN=AS2 PE=2 S... [more]
Match NameE-valueIdentityDescription
A0A061GZV4_THECC6.7e-24587.26Glutamine-dependent asparagine synthase 1 OS=Theobroma cacao GN=TCM_041592 PE=4 ... [more]
A0A067LBF0_JATCU1.5e-24486.08Uncharacterized protein OS=Jatropha curcas GN=JCGZ_17398 PE=4 SV=1[more]
G3G8K0_VITVI2.0e-24487.61Asparagine synthetase 1 OS=Vitis vinifera PE=2 SV=1[more]
D7SJV6_VITVI2.0e-24487.61Putative uncharacterized protein OS=Vitis vinifera GN=VIT_06s0004g06830 PE=4 SV=... [more]
B9SL40_RICCO6.3e-24386.13Asparagine synthetase, putative OS=Ricinus communis GN=RCOM_0479760 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G47340.19.6e-23582.39 glutamine-dependent asparagine synthase 1[more]
AT5G10240.11.3e-22079.19 asparagine synthetase 3[more]
AT5G65010.21.8e-22077.22 asparagine synthetase 2[more]
Match NameE-valueIdentityDescription
gi|778715252|ref|XP_004144212.2|1.9e-26193.64PREDICTED: LOW QUALITY PROTEIN: asparagine synthetase [glutamine-hydrolyzing][more]
gi|590587838|ref|XP_007016070.1|9.7e-24587.26Glutamine-dependent asparagine synthase 1 [Theobroma cacao][more]
gi|802541023|ref|XP_012079122.1|2.1e-24486.08PREDICTED: asparagine synthetase [glutamine-hydrolyzing][more]
gi|1009115914|ref|XP_015874493.1|2.1e-24485.77PREDICTED: asparagine synthetase [glutamine-hydrolyzing][more]
gi|297745876|emb|CBI15932.3|2.8e-24487.61unnamed protein product [Vitis vinifera][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001962Asn_synthase
IPR014729Rossmann-like_a/b/a_fold
IPR017932GATase_2_dom
Vocabulary: Molecular Function
TermDefinition
GO:0004066asparagine synthase (glutamine-hydrolyzing) activity
Vocabulary: Biological Process
TermDefinition
GO:0006529asparagine biosynthetic process
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006522 alanine metabolic process
biological_process GO:0006529 asparagine biosynthetic process
biological_process GO:0006531 aspartate metabolic process
biological_process GO:0009063 cellular amino acid catabolic process
biological_process GO:0009646 response to absence of light
biological_process GO:0006541 glutamine metabolic process
biological_process GO:0043617 cellular response to sucrose starvation
biological_process GO:0009750 response to fructose
biological_process GO:0009749 response to glucose
biological_process GO:0009744 response to sucrose
cellular_component GO:0005575 cellular_component
cellular_component GO:0005829 cytosol
molecular_function GO:0004066 asparagine synthase (glutamine-hydrolyzing) activity
molecular_function GO:0042803 protein homodimerization activity
molecular_function GO:0005524 ATP binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO3C026828T1MELO3C026828T1mRNA


Analysis Name: InterPro Annotations of melon
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001962Asparagine synthasePFAMPF00733Asn_synthasecoord: 95..246
score: 6.1
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3DG3DSA:3.40.50.620coord: 76..395
score: 2.2E
IPR017932Glutamine amidotransferase type 2 domainPFAMPF13537GATase_7coord: 2..50
score: 5.7
IPR017932Glutamine amidotransferase type 2 domainPROFILEPS51278GATASE_TYPE_2coord: 1..70
score: 10
NoneNo IPR availablePANTHERPTHR11772ASPARAGINE SYNTHETASEcoord: 1..423
score:
NoneNo IPR availablePANTHERPTHR11772:SF13ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING] 2-RELATEDcoord: 1..423
score:
NoneNo IPR availableunknownSSF52402Adenine nucleotide alpha hydrolases-likecoord: 94..365
score: 3.09