BLAST of MELO3C023378 vs. Swiss-Prot
Match:
G3OX_PEA (Gibberellin 3-beta-dioxygenase 1 OS=Pisum sativum GN=LE PE=1 SV=1)
HSP 1 Score: 459.1 bits (1180), Expect = 4.5e-128
Identity = 222/373 (59.52%), Postives = 278/373 (74.53%), Query Frame = 1
Query: 7 ITEVFKSHPVHIPIHKNFDFDSLQELPDSYDWIQPDSFPNSSLSNNHLSDS---IPLIDL 66
++E +++HPVH+ HK+ DF+SLQELP+SY+W D SNN + +S +P+IDL
Sbjct: 4 LSEAYRAHPVHVN-HKHPDFNSLQELPESYNWTHLDDHTLID-SNNIMKESTTTVPVIDL 63
Query: 67 SLPNAPHLIGNAFRTWGAFQVINHGVPISLLHSIESSANSLFSLPAPHKLKAARPSDGIS 126
+ PNA LIG A +TWG +QV+NHG+P+SLL I+ +LFSLP+ K KA R DG+S
Sbjct: 64 NDPNASKLIGLACKTWGVYQVMNHGIPLSLLEDIQWLGQTLFSLPSHQKHKATRSPDGVS 123
Query: 127 GYGLVRISSFFPKRMWSEGFTIVGSPLEHFRKLWPHDYTRYCDIVEEYDQEMKSLCGRLM 186
GYG+ RISSFFPK MW EGFTIVGSPL+HFR+LWP DYTR+CDIV +YD+ MK L G LM
Sbjct: 124 GYGIARISSFFPKLMWYEGFTIVGSPLDHFRELWPQDYTRFCDIVVQYDETMKKLAGTLM 183
Query: 187 WVALGELGITREDVNWAGPNGDFMTSNAATQFNSYPVCPDPERAMGLGAHTDTSLLTIVY 246
+ L LGIT+ED+ WAG F + AA Q NSYP CPDP+ AMGL HTD++ LTI+
Sbjct: 184 CLMLDSLGITKEDIKWAGSKAQFEKACAALQLNSYPSCPDPDHAMGLAPHTDSTFLTILS 243
Query: 247 QNNTRGLQVLREGNRWVTVEPVPGALVVQVGDLLHILTNGLYPSPVHQAVVNRTRKRLSV 306
QN+ GLQV REG+ W+TV P+ G LVV VGDL HIL+NGLYPS +H+ +VNRTR+R SV
Sbjct: 244 QNDISGLQVNREGSGWITVPPLQGGLVVNVGDLFHILSNGLYPSVLHRVLVNRTRQRFSV 303
Query: 307 AYVFGPPESAEISPLKKLLGPTQPPLYRTVTWTEYLRKKAEHFNNALSSVRLCAPLTGLL 366
AY++GPP + EI P KL+GPT+PPLYR+VTW EYL KA+HFN ALSSVRLC P+ GL
Sbjct: 304 AYLYGPPSNVEICPHAKLIGPTKPPLYRSVTWNEYLGTKAKHFNKALSSVRLCTPINGLF 363
Query: 367 DVNDHSQ--VKVG 375
DVND ++ V+VG
Sbjct: 364 DVNDSNKNSVQVG 374
BLAST of MELO3C023378 vs. Swiss-Prot
Match:
G3OX1_ARATH (Gibberellin 3-beta-dioxygenase 1 OS=Arabidopsis thaliana GN=GA3OX1 PE=1 SV=2)
HSP 1 Score: 422.2 bits (1084), Expect = 6.1e-117
Identity = 202/349 (57.88%), Postives = 251/349 (71.92%), Query Frame = 1
Query: 7 ITEVFKSHPVHIPIHKNFDFDSLQELPDSYDWIQPDSFPNSSL-SNNHLSDSIPLIDLSL 66
+T+VF+ HP+H+P DF SL+ELPDSY W D S+ S ++IPLIDL
Sbjct: 5 LTDVFRGHPIHLPHSHIPDFTSLRELPDSYKWTPKDDLLFSAAPSPPATGENIPLIDLDH 64
Query: 67 PNAPHLIGNAFRTWGAFQVINHGVPISLLHSIESSANSLFSLPAPHKLKAARPSDGISGY 126
P+A + IG+A RTWGAFQ+ NHGVP+ LL IE SLF LP KLK+AR G+SGY
Sbjct: 65 PDATNQIGHACRTWGAFQISNHGVPLGLLQDIEFLTGSLFGLPVQRKLKSARSETGVSGY 124
Query: 127 GLVRISSFFPKRMWSEGFTIVGSPLEHFRKLWPHDYTRYCDIVEEYDQEMKSLCGRLMWV 186
G+ RI+SFF K+MWSEGFTI GSPL FRKLWP + YCDIVEEY++ MK L +LMW+
Sbjct: 125 GVARIASFFNKQMWSEGFTITGSPLNDFRKLWPQHHLNYCDIVEEYEEHMKKLASKLMWL 184
Query: 187 ALGELGITREDVNWAGPNGDFMTSNAATQFNSYPVCPDPERAMGLGAHTDTSLLTIVYQN 246
AL LG++ ED+ WA + D + AA Q N YPVCP+P+RAMGL AHTD++LLTI+YQN
Sbjct: 185 ALNSLGVSEEDIEWASLSSDLNWAQAALQLNHYPVCPEPDRAMGLAAHTDSTLLTILYQN 244
Query: 247 NTRGLQVLREGNRWVTVEPVPGALVVQVGDLLHILTNGLYPSPVHQAVVNRTRKRLSVAY 306
NT GLQV R+ WVTV P PG+LVV VGDL HIL+NGL+ S +H+A VN+TR RLSVA+
Sbjct: 245 NTAGLQVFRDDLGWVTVPPFPGSLVVNVGDLFHILSNGLFKSVLHRARVNQTRARLSVAF 304
Query: 307 VFGPPESAEISPLKKLLGPTQPPLYRTVTWTEYLRKKAEHFNNALSSVR 355
++GP +ISP+ KL+ P + PLY++VTW EYLR KA HFN ALS +R
Sbjct: 305 LWGPQSDIKISPVPKLVSPVESPLYQSVTWKEYLRTKATHFNKALSMIR 353
BLAST of MELO3C023378 vs. Swiss-Prot
Match:
G3OX2_ARATH (Gibberellin 3-beta-dioxygenase 2 OS=Arabidopsis thaliana GN=GA3OX2 PE=1 SV=2)
HSP 1 Score: 413.3 bits (1061), Expect = 2.8e-114
Identity = 194/348 (55.75%), Postives = 249/348 (71.55%), Query Frame = 1
Query: 7 ITEVFKSHPVHIPIHKNFDFDSLQELPDSYDWIQPDSFPNSSLSNNHLSDSIPLIDLSLP 66
+++VF+SHP+HIP+ DF SL PDSY W D S+ +++ +++PLIDLS
Sbjct: 5 LSDVFRSHPIHIPLSNPPDFKSL---PDSYTWTPKDDLLFSASASD---ETLPLIDLSDI 64
Query: 67 NAPHLIGNAFRTWGAFQVINHGVPISLLHSIESSANSLFSLPAPHKLKAARPSDGISGYG 126
+ L+G+A TWGAFQ+ NHGVP LL IE SLF LP KLKAAR +G+SGYG
Sbjct: 65 HVATLVGHACTTWGAFQITNHGVPSRLLDDIEFLTGSLFRLPVQRKLKAARSENGVSGYG 124
Query: 127 LVRISSFFPKRMWSEGFTIVGSPLEHFRKLWPHDYTRYCDIVEEYDQEMKSLCGRLMWVA 186
+ RI+SFF K+MWSEGFT++GSPL FRKLWP + +YC+I+EEY++ M+ L +LMW A
Sbjct: 125 VARIASFFNKKMWSEGFTVIGSPLHDFRKLWPSHHLKYCEIIEEYEEHMQKLAAKLMWFA 184
Query: 187 LGELGITREDVNWAGPNGDFMTSNAATQFNSYPVCPDPERAMGLGAHTDTSLLTIVYQNN 246
LG LG+ +D+ WAGPN DF + A Q N YP CP+P+RAMGL AHTD++L+TI+YQNN
Sbjct: 185 LGSLGVEEKDIQWAGPNSDFQGTQAVIQLNHYPKCPEPDRAMGLAAHTDSTLMTILYQNN 244
Query: 247 TRGLQVLREGNRWVTVEPVPGALVVQVGDLLHILTNGLYPSPVHQAVVNRTRKRLSVAYV 306
T GLQV R+ WVT PVPG+LVV VGDLLHILTNG++PS +H+A VN R R S+AY+
Sbjct: 245 TAGLQVFRDDVGWVTAPPVPGSLVVNVGDLLHILTNGIFPSVLHRARVNHVRSRFSMAYL 304
Query: 307 FGPPESAEISPLKKLLGPTQPPLYRTVTWTEYLRKKAEHFNNALSSVR 355
+GPP ISPL KL+ P Q PLY ++TW +YL KA HFN +LS +R
Sbjct: 305 WGPPSDIMISPLPKLVDPLQSPLYPSLTWKQYLATKATHFNQSLSIIR 346
BLAST of MELO3C023378 vs. Swiss-Prot
Match:
G3OX4_ARATH (Gibberellin 3-beta-dioxygenase 4 OS=Arabidopsis thaliana GN=GA3OX4 PE=1 SV=1)
HSP 1 Score: 356.3 bits (913), Expect = 4.1e-97
Identity = 177/339 (52.21%), Postives = 233/339 (68.73%), Query Frame = 1
Query: 28 SLQELPDSYDW--IQPDSF--PNSSLSNNHLSDSIPLIDLSLPNAPHLIGNAFRTWGAFQ 87
SLQ LP+S+ W DS P+S++S + + +SIP+IDLS P+ LIG+A +TWGAFQ
Sbjct: 15 SLQTLPESFTWKLTAADSLLRPSSAVSFDAVEESIPVIDLSNPDVTTLIGDASKTWGAFQ 74
Query: 88 VINHGVPISLLHSIESSANSLFSLPAPHKLKAARPSDGISGYGLVRISSFFPKRMWSEGF 147
+ NHG+ LL IES + +LF +P+ KL+AA G+SGYG RIS FF K+MWSEGF
Sbjct: 75 IANHGISQKLLDDIESLSKTLFDMPSERKLEAASSDKGVSGYGEPRISPFFEKKMWSEGF 134
Query: 148 TIVG-SPLEHFRKLWPHDYTRYCDIVEEYDQEMKSLCGRLMWVALGELGITREDVNWAGP 207
TI S HF LWPHD+T+YC I++EY EM+ L RL++ LG LG+T ED+ WA
Sbjct: 135 TIADDSYRNHFNTLWPHDHTKYCGIIQEYVDEMEKLASRLLYCILGSLGVTVEDIEWAHK 194
Query: 208 --NGDFMTSNAATQFNSYPVCPDPERAMGLGAHTDTSLLTIVYQNNTRGLQVLREGNRWV 267
A + N YPVCP+PERAMGL AHTD+++LTI++Q+NT GLQV RE + WV
Sbjct: 195 LEKSGSKVGRGAIRLNHYPVCPEPERAMGLAAHTDSTILTILHQSNTGGLQVFREESGWV 254
Query: 268 TVEPVPGALVVQVGDLLHILTNGLYPSPVHQAVVNRTRKRLSVAYVF-GPPESAEISPLK 327
TVEP PG LVV +GDL HIL+NG PS VH+A VN TR R+S+AY++ GP +I+P+
Sbjct: 255 TVEPAPGVLVVNIGDLFHILSNGKIPSVVHRAKVNHTRSRISIAYLWGGPAGDVQIAPIS 314
Query: 328 KLLGPTQPPLYRTVTWTEYLRKKAEHFNNALSSVRLCAP 359
KL GP +P LYR++TW EYL+ K E F+ A+ ++R+ P
Sbjct: 315 KLTGPAEPSLYRSITWKEYLQIKYEVFDKAMDAIRVVNP 353
BLAST of MELO3C023378 vs. Swiss-Prot
Match:
G3OX3_ARATH (Gibberellin 3-beta-dioxygenase 3 OS=Arabidopsis thaliana GN=GA3OX3 PE=2 SV=1)
HSP 1 Score: 306.6 bits (784), Expect = 3.7e-82
Identity = 159/355 (44.79%), Postives = 221/355 (62.25%), Query Frame = 1
Query: 7 ITEVFKSHPVHIPIHKNFDFDSLQELPDSYDWIQPDSFPNSSLSNNHLSDSIPLIDLSLP 66
+T++FK++PV+ DF + + LPDS+ W +P+ P ++ S IP+I LS P
Sbjct: 4 VTQLFKNNPVNRDRIIPLDFTNTKTLPDSHVWSKPE--PETT------SGPIPVISLSNP 63
Query: 67 NAPHLIGNAFRTWGAFQVINHGVPISLLHSIESSANSLFSLPAPHKLKAARPSDGISGYG 126
L+ A WG F + +HGV SLLH+++ LFSLP K+ A R D +GYG
Sbjct: 64 EEHGLLRQACEEWGVFHITDHGVSHSLLHNVDCQMKRLFSLPMHRKILAVRSPDESTGYG 123
Query: 127 LVRISSFFPKRMWSEGFTIVGSPL-EHFRKLWPHDYTRYCDIVEEYDQEMKSLCGRLMWV 186
+VRIS F+ K MWSEGF+++GS L H LWP D+ +C+++EEY + M L RL+ +
Sbjct: 124 VVRISMFYDKLMWSEGFSVMGSSLRRHATLLWPDDHAEFCNVMEEYQKAMDDLSHRLISM 183
Query: 187 ALGELGITREDVNWAGPNGDFMTSNAATQF---NSYPVCPDPERAMGLGAHTDTSLLTIV 246
+G LG+T ED+ W P+ +++ F NSYPVCPDP AMGL HTD+SLLTI+
Sbjct: 184 LMGSLGLTHEDLGWLVPDKTGSGTDSIQSFLQLNSYPVCPDPHLAMGLAPHTDSSLLTIL 243
Query: 247 YQNNTRGLQV---LREGNRWVTVEPVPGALVVQVGDLLHILTNGLYPSPVHQAVVNRTRK 306
YQ N GL++ EG+RW+ VEP+ G+LVV +GDL HI++NG + S +H+AVVN+T
Sbjct: 244 YQGNIPGLEIESPQEEGSRWIGVEPIEGSLVVIMGDLSHIISNGQFRSTMHRAVVNKTHH 303
Query: 307 RLSVAYVFGPPESAEISPLKKLLGPTQPPLYRTVTWTEYLRKKAEHFNNALSSVR 355
R+S AY GPP++ +I PL PP+YR + W EYL KA HFN AL+ R
Sbjct: 304 RVSAAYFAGPPKNLQIGPLTS--DKNHPPIYRRLIWEEYLAAKATHFNKALTLFR 348
BLAST of MELO3C023378 vs. TrEMBL
Match:
S0BE27_CUCSA (GA 3-oxidase OS=Cucumis sativus GN=GA3ox3 PE=2 SV=1)
HSP 1 Score: 604.7 bits (1558), Expect = 7.4e-170
Identity = 286/368 (77.72%), Postives = 326/368 (88.59%), Query Frame = 1
Query: 6 KITEVFKSHPVHIPIHKNFDFDSLQELPDSYDWIQPDSFPNSSLSNNHLSDSIPLIDLSL 65
K+T+++K+HP+ IP +K + DSLQ+LPD+YDWIQPD+FP+ N LSDS+PLIDLSL
Sbjct: 8 KLTDIYKTHPLDIPTNK-LNLDSLQQLPDTYDWIQPDAFPSFRDPNISLSDSVPLIDLSL 67
Query: 66 PNAPHLIGNAFRTWGAFQVINHGVPISLLHSIESSANSLFSLPAPHKLKAARPSDGISGY 125
PNAPHLIGNA +TWG FQVINHGVPISLL+S+ES N+LF LP+PHKLKAAR DG++GY
Sbjct: 68 PNAPHLIGNALKTWGVFQVINHGVPISLLNSMESFTNTLFDLPSPHKLKAARTPDGVTGY 127
Query: 126 GLVRISSFFPKRMWSEGFTIVGSPLEHFRKLWPHDYTRYCDIVEEYDQEMKSLCGRLMWV 185
GL RIS+FFPKRMWSEGFTIVGSPLEHF+KLWP++Y++YCDI EEY +EMKSLCGR++W+
Sbjct: 128 GLPRISTFFPKRMWSEGFTIVGSPLEHFQKLWPNEYSKYCDITEEYSREMKSLCGRVIWL 187
Query: 186 ALGELGITREDVNWAGPNGDFMTSNAATQFNSYPVCPDPERAMGLGAHTDTSLLTIVYQN 245
ALGELGITREDVNWA NGDF TSNA Q NSYPVCPDP+RAMGLGAHTDTSLLTI+YQN
Sbjct: 188 ALGELGITREDVNWAEANGDFKTSNAVIQLNSYPVCPDPDRAMGLGAHTDTSLLTILYQN 247
Query: 246 NTRGLQVLREGNRWVTVEPVPGALVVQVGDLLHILTNGLYPSPVHQAVVNRTRKRLSVAY 305
NTRGLQVLREGNRWV VEPV GALVVQVGDLLHILTNGLYPS HQAVVN+ RKR+S+AY
Sbjct: 248 NTRGLQVLREGNRWVMVEPVTGALVVQVGDLLHILTNGLYPSSAHQAVVNQNRKRISIAY 307
Query: 306 VFGPPESAEISPLKKLLGPTQPPLYRTVTWTEYLRKKAEHFNNALSSVRLCAPLTGLLDV 365
FGP ESAEISPL KL+ PTQP LY TVTWTEYLRKKA+ FNN LSS+RL APLTG LD+
Sbjct: 308 FFGPSESAEISPLNKLVTPTQPLLYPTVTWTEYLRKKAQLFNNTLSSIRLSAPLTGALDI 367
Query: 366 NDHSQVKV 374
NDH+QVKV
Sbjct: 368 NDHNQVKV 374
BLAST of MELO3C023378 vs. TrEMBL
Match:
E1AXG5_9ROSI (Gibberellin 3-oxidase 2 (Fragment) OS=Marah macrocarpus PE=2 SV=1)
HSP 1 Score: 590.9 bits (1522), Expect = 1.1e-165
Identity = 281/370 (75.95%), Postives = 318/370 (85.95%), Query Frame = 1
Query: 6 KITEVFKSHPVHIPIHKNFDFDSLQELPDSYDWIQPDSFPNSSLSNNHLSDSIPLIDLSL 65
KIT+ FKSHPVH+P HKN DFDS ELPD+Y+W+QPDS P+ N+ + +PLIDLSL
Sbjct: 4 KITDAFKSHPVHVPAHKNLDFDSFNELPDTYEWVQPDSIPSP---NDQSQEHLPLIDLSL 63
Query: 66 PNAPHLIGNAFRTWGAFQVINHGVPISLLHSIESSANSLFSLPAPHKLKAARPSDGISGY 125
PNA L+G+AFR+WGAFQ INHGVPISLLHS ES+ ++LFSLP K KAAR DG+ GY
Sbjct: 64 PNAAALVGDAFRSWGAFQAINHGVPISLLHSFESTVDTLFSLPPSDKHKAARTPDGVDGY 123
Query: 126 GLVRISSFFPKRMWSEGFTIVGSPLEHFRKLWPHDYTRYCDIVEEYDQEMKSLCGRLMWV 185
GLVRISSFFPKRMWSEGFTI+ SPL HF+KLWP DY ++C+I EEYD+EM+SLC RL+W+
Sbjct: 124 GLVRISSFFPKRMWSEGFTILNSPLHHFQKLWPDDYAKHCEIYEEYDREMRSLCARLIWL 183
Query: 186 ALGELGITREDVNWAGPNGDFMTSNAATQFNSYPVCPDPERAMGLGAHTDTSLLTIVYQN 245
LGELGIT++DV AGP+ DF T+ AATQ NSYP+CPDPERAMGLGAHTDTSLLTIVYQN
Sbjct: 184 GLGELGITQDDVELAGPDRDFKTNPAATQLNSYPICPDPERAMGLGAHTDTSLLTIVYQN 243
Query: 246 NTRGLQVLREGNRWVTVEPVPGALVVQVGDLLHILTNGLYPSPVHQAVVNRTRKRLSVAY 305
NT GLQ LREGNRWVTVEPV GALVVQVGDLL I+TNGLY SP+HQA VNRTRKRLS+AY
Sbjct: 244 NTSGLQFLREGNRWVTVEPVDGALVVQVGDLLQIMTNGLYKSPLHQAFVNRTRKRLSMAY 303
Query: 306 VFGPPESAEISPLKKLLGPTQPPLYRTVTWTEYLRKKAEHFNNALSSVRLCAP-LTGLLD 365
+FG PE+AEISPLKKLLGPTQPP+YRTVTWTEYL KKAEHFNNAL SVR+CAP GLLD
Sbjct: 304 IFGTPETAEISPLKKLLGPTQPPIYRTVTWTEYLGKKAEHFNNALKSVRICAPSANGLLD 363
Query: 366 VNDHSQVKVG 375
VND SQVKVG
Sbjct: 364 VNDQSQVKVG 370
BLAST of MELO3C023378 vs. TrEMBL
Match:
A0A0A0KAA7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G435470 PE=3 SV=1)
HSP 1 Score: 542.0 bits (1395), Expect = 5.9e-151
Identity = 261/375 (69.60%), Postives = 302/375 (80.53%), Query Frame = 1
Query: 6 KITEVFKSHPVHIPIHKNFDFDSLQELPDSYDWIQPDSFPN-------SSLSNNHLSDSI 65
K+ E +K+ PVH +KN D S +++PDS+DW+QP+SFP+ S LS++ +DS+
Sbjct: 6 KMIEAYKTDPVHALTNKNLD--SFEQIPDSHDWVQPNSFPSFTDQSNISDLSSD--TDSV 65
Query: 66 PLIDLSLPNAPHLIGNAFRTWGAFQVINHGVPISLLHSIESSANSLFSLPAPHKLKAARP 125
PLIDLSLPNAP LIGNA RTWG FQ INHGVPIS+L+S+ES N LF LP P KLKAAR
Sbjct: 66 PLIDLSLPNAPKLIGNALRTWGVFQAINHGVPISVLNSMESLLNDLFDLPTPQKLKAARS 125
Query: 126 SDGISGYGLVRISSFFPKRMWSEGFTIVGSPLEHFRKLWPHDYTRYCDIVEEYDQEMKSL 185
DG++GYG RIS+FFPK MWSEGFT+ GSPLEHF+ LWPHD T+YCDI+EEYD+EMK L
Sbjct: 126 RDGVNGYGRFRISTFFPKSMWSEGFTVSGSPLEHFQILWPHDCTKYCDIIEEYDREMKGL 185
Query: 186 CGRLMWVALGELGITREDVNWAGPNGDFMTSNAATQFNSYPVCPDPERAMGLGAHTDTSL 245
CGR++W+ALGELGITREDVNWAGPNGDF TS+ NSYPVCPDP+RAMG+G HTD+
Sbjct: 186 CGRVVWLALGELGITREDVNWAGPNGDFPTSSGVMNLNSYPVCPDPDRAMGIGVHTDSCF 245
Query: 246 LTIVYQNNTRGLQVLREGNRWVTVEPVPGALVVQVGDLLHILTNGLYPSPVHQAVVNRTR 305
LT++YQNN GLQVLREG RWVTV+PVPGALVVQV DLL ILTNGLY SP HQAVVNR R
Sbjct: 246 LTLLYQNNASGLQVLREGKRWVTVDPVPGALVVQVADLLQILTNGLYSSPFHQAVVNRDR 305
Query: 306 KRLSVAYVFGPPESAEISPLKKLLGPTQPPLYRTVTWTEYLRKKAEHFNNALSSVRLCAP 365
KRLSVAY FGPP AEISP+KKL+ PTQP LY TVTW EYL KKAE FN+ L S+RL P
Sbjct: 306 KRLSVAYFFGPPVHAEISPIKKLVNPTQPLLYPTVTWAEYLCKKAELFNDTLPSIRLSTP 365
Query: 366 LTGLLDVNDHSQVKV 374
T DVNDHSQVK+
Sbjct: 366 PTESSDVNDHSQVKI 376
BLAST of MELO3C023378 vs. TrEMBL
Match:
M5XCD8_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa007336mg PE=3 SV=1)
HSP 1 Score: 530.4 bits (1365), Expect = 1.8e-147
Identity = 252/370 (68.11%), Postives = 301/370 (81.35%), Query Frame = 1
Query: 6 KITEVFKSHPVHIPIHKNFDFDSLQELPDSYDWIQ-PDSFPNSSLSNNHLSDSIPLIDLS 65
++++ FKSHP+++ + K+ DF SLQELPDSY W Q D +P+S S DS+P+IDLS
Sbjct: 4 RLSDAFKSHPLNLQL-KHPDFSSLQELPDSYAWSQNDDQYPSSPGSFGAEQDSVPVIDLS 63
Query: 66 LPNAPHLIGNAFRTWGAFQVINHGVPISLLHSIESSANSLFSLPAPHKLKAARPSDGISG 125
PNA L G+A RTWG FQV NHG+P LL IES+ SLFSLP KLKAAR DGISG
Sbjct: 64 DPNALKLTGHACRTWGVFQVTNHGIPSKLLDDIESAGRSLFSLPVQQKLKAARSPDGISG 123
Query: 126 YGLVRISSFFPKRMWSEGFTIVGSPLEHFRKLWPHDYTRYCDIVEEYDQEMKSLCGRLMW 185
YG RISSFF K MWSEGFTIVGSPL+HFR+LWP DY ++C+I+EEY++EMK L GRLMW
Sbjct: 124 YGFARISSFFQKLMWSEGFTIVGSPLDHFRQLWPQDYNKFCNIIEEYEKEMKKLAGRLMW 183
Query: 186 VALGELGITREDVNWAGPNGDFMTSNAATQFNSYPVCPDPERAMGLGAHTDTSLLTIVYQ 245
+ LG LGI++EDV WAGP GDF ++AA Q NSYP CPDP+RAMGL AHTD++LLTI+YQ
Sbjct: 184 LMLGSLGISKEDVKWAGPKGDFRDASAALQLNSYPACPDPDRAMGLAAHTDSTLLTILYQ 243
Query: 246 NNTRGLQVLREGNRWVTVEPVPGALVVQVGDLLHILTNGLYPSPVHQAVVNRTRKRLSVA 305
NNT GLQVLREG RWVTV P+PGALVV VGDL+HIL+NGLYPS +HQAVVNR+R RLS+A
Sbjct: 244 NNTSGLQVLREGTRWVTVPPLPGALVVNVGDLIHILSNGLYPSVLHQAVVNRSRHRLSIA 303
Query: 306 YVFGPPESAEISPLKKLLGPTQPPLYRTVTWTEYLRKKAEHFNNALSSVRLCAPLTGLLD 365
Y++GPP+S +ISPL KLLGP+QPPLYR +TW EYL KA+HFN ALSSVRLCAPL L+D
Sbjct: 304 YLYGPPDSVQISPLSKLLGPSQPPLYRPITWNEYLGTKAKHFNKALSSVRLCAPLNALVD 363
Query: 366 VNDHSQVKVG 375
V+DH+ VKVG
Sbjct: 364 VSDHNSVKVG 372
BLAST of MELO3C023378 vs. TrEMBL
Match:
Q9M4P2_CUCMA (Gibberellin 3-beta-hydroxylase OS=Cucurbita maxima PE=2 SV=1)
HSP 1 Score: 521.2 bits (1341), Expect = 1.1e-144
Identity = 246/361 (68.14%), Postives = 296/361 (81.99%), Query Frame = 1
Query: 14 HPVHIPIHKNFDFDSLQELPDSYDWIQPDSFPNSSLSNNHLSDSIPLIDLSLPNAPHLIG 73
H VHI HK+ D DSLQ LPDSY W+QPD FP+ + LSD++PLIDLSLP+AP LIG
Sbjct: 8 HSVHIT-HKDHDLDSLQVLPDSYQWVQPDLFPSLQQHSTPLSDTLPLIDLSLPDAPDLIG 67
Query: 74 NAFRTWGAFQVINHGVPISLLHSIESSANSLFSLPAPHKLKAARPSDGISGYGLVRISSF 133
A RTWG+FQ+INHGVP+SL+H+ +SSA LF+LP P+KLKA+R GYG+ RI+SF
Sbjct: 68 KALRTWGSFQIINHGVPLSLIHAAQSSAIDLFALPTPYKLKASRTPGTFPGYGIARINSF 127
Query: 134 FPKRMWSEGFTIVGSPLEHFRKLWPHDYTRYCDIVEEYDQEMKSLCGRLMWVALGELGIT 193
FPKRMWSE FTI+GSPLEHFR LWPHDY++ CDI+ EYD+E+KSLCGRLMW+AL ELGIT
Sbjct: 128 FPKRMWSESFTIMGSPLEHFRHLWPHDYSKRCDIMAEYDRELKSLCGRLMWIALSELGIT 187
Query: 194 REDVNWAGPNGDFMTSNAATQFNSYPVCPDPERAMGLGAHTDTSLLTIVYQNNTRGLQVL 253
RED+NWAGPN DF T NAATQ N YP CPDP+RAMGLGAHTDT+LLT+++QNNTRGLQ+L
Sbjct: 188 REDINWAGPNEDFKTCNAATQLNFYPACPDPDRAMGLGAHTDTTLLTVLHQNNTRGLQIL 247
Query: 254 REGNRWVTVEPVPGALVVQVGDLLHILTNGLYPSPVHQAVVNRTRKRLSVAYVFGPPESA 313
REG +WVTVEPV GALVVQVGD+L I++NGLY SP HQAVVNRT+KRLSVAY+F PPE+
Sbjct: 248 REGRQWVTVEPVAGALVVQVGDMLQIVSNGLYKSPFHQAVVNRTQKRLSVAYLFVPPENV 307
Query: 314 EISPLKKLLGPTQPPLYRTVTWTEYLRKKAEHFNNALSSVRLCAPLTGLLDVNDHSQVKV 373
E+SP KKLL PTQPPLYR ++WT+++R K+ +F + LS +R +DV D QVKV
Sbjct: 308 EMSPHKKLLNPTQPPLYRPISWTDFIRIKSNNF-DGLSCIR--------VDVKDEKQVKV 358
Query: 374 G 375
G
Sbjct: 368 G 358
BLAST of MELO3C023378 vs. TAIR10
Match:
AT1G15550.1 (AT1G15550.1 gibberellin 3-oxidase 1)
HSP 1 Score: 422.2 bits (1084), Expect = 3.4e-118
Identity = 202/349 (57.88%), Postives = 251/349 (71.92%), Query Frame = 1
Query: 7 ITEVFKSHPVHIPIHKNFDFDSLQELPDSYDWIQPDSFPNSSL-SNNHLSDSIPLIDLSL 66
+T+VF+ HP+H+P DF SL+ELPDSY W D S+ S ++IPLIDL
Sbjct: 5 LTDVFRGHPIHLPHSHIPDFTSLRELPDSYKWTPKDDLLFSAAPSPPATGENIPLIDLDH 64
Query: 67 PNAPHLIGNAFRTWGAFQVINHGVPISLLHSIESSANSLFSLPAPHKLKAARPSDGISGY 126
P+A + IG+A RTWGAFQ+ NHGVP+ LL IE SLF LP KLK+AR G+SGY
Sbjct: 65 PDATNQIGHACRTWGAFQISNHGVPLGLLQDIEFLTGSLFGLPVQRKLKSARSETGVSGY 124
Query: 127 GLVRISSFFPKRMWSEGFTIVGSPLEHFRKLWPHDYTRYCDIVEEYDQEMKSLCGRLMWV 186
G+ RI+SFF K+MWSEGFTI GSPL FRKLWP + YCDIVEEY++ MK L +LMW+
Sbjct: 125 GVARIASFFNKQMWSEGFTITGSPLNDFRKLWPQHHLNYCDIVEEYEEHMKKLASKLMWL 184
Query: 187 ALGELGITREDVNWAGPNGDFMTSNAATQFNSYPVCPDPERAMGLGAHTDTSLLTIVYQN 246
AL LG++ ED+ WA + D + AA Q N YPVCP+P+RAMGL AHTD++LLTI+YQN
Sbjct: 185 ALNSLGVSEEDIEWASLSSDLNWAQAALQLNHYPVCPEPDRAMGLAAHTDSTLLTILYQN 244
Query: 247 NTRGLQVLREGNRWVTVEPVPGALVVQVGDLLHILTNGLYPSPVHQAVVNRTRKRLSVAY 306
NT GLQV R+ WVTV P PG+LVV VGDL HIL+NGL+ S +H+A VN+TR RLSVA+
Sbjct: 245 NTAGLQVFRDDLGWVTVPPFPGSLVVNVGDLFHILSNGLFKSVLHRARVNQTRARLSVAF 304
Query: 307 VFGPPESAEISPLKKLLGPTQPPLYRTVTWTEYLRKKAEHFNNALSSVR 355
++GP +ISP+ KL+ P + PLY++VTW EYLR KA HFN ALS +R
Sbjct: 305 LWGPQSDIKISPVPKLVSPVESPLYQSVTWKEYLRTKATHFNKALSMIR 353
BLAST of MELO3C023378 vs. TAIR10
Match:
AT1G80340.1 (AT1G80340.1 gibberellin 3-oxidase 2)
HSP 1 Score: 413.3 bits (1061), Expect = 1.6e-115
Identity = 194/348 (55.75%), Postives = 249/348 (71.55%), Query Frame = 1
Query: 7 ITEVFKSHPVHIPIHKNFDFDSLQELPDSYDWIQPDSFPNSSLSNNHLSDSIPLIDLSLP 66
+++VF+SHP+HIP+ DF SL PDSY W D S+ +++ +++PLIDLS
Sbjct: 5 LSDVFRSHPIHIPLSNPPDFKSL---PDSYTWTPKDDLLFSASASD---ETLPLIDLSDI 64
Query: 67 NAPHLIGNAFRTWGAFQVINHGVPISLLHSIESSANSLFSLPAPHKLKAARPSDGISGYG 126
+ L+G+A TWGAFQ+ NHGVP LL IE SLF LP KLKAAR +G+SGYG
Sbjct: 65 HVATLVGHACTTWGAFQITNHGVPSRLLDDIEFLTGSLFRLPVQRKLKAARSENGVSGYG 124
Query: 127 LVRISSFFPKRMWSEGFTIVGSPLEHFRKLWPHDYTRYCDIVEEYDQEMKSLCGRLMWVA 186
+ RI+SFF K+MWSEGFT++GSPL FRKLWP + +YC+I+EEY++ M+ L +LMW A
Sbjct: 125 VARIASFFNKKMWSEGFTVIGSPLHDFRKLWPSHHLKYCEIIEEYEEHMQKLAAKLMWFA 184
Query: 187 LGELGITREDVNWAGPNGDFMTSNAATQFNSYPVCPDPERAMGLGAHTDTSLLTIVYQNN 246
LG LG+ +D+ WAGPN DF + A Q N YP CP+P+RAMGL AHTD++L+TI+YQNN
Sbjct: 185 LGSLGVEEKDIQWAGPNSDFQGTQAVIQLNHYPKCPEPDRAMGLAAHTDSTLMTILYQNN 244
Query: 247 TRGLQVLREGNRWVTVEPVPGALVVQVGDLLHILTNGLYPSPVHQAVVNRTRKRLSVAYV 306
T GLQV R+ WVT PVPG+LVV VGDLLHILTNG++PS +H+A VN R R S+AY+
Sbjct: 245 TAGLQVFRDDVGWVTAPPVPGSLVVNVGDLLHILTNGIFPSVLHRARVNHVRSRFSMAYL 304
Query: 307 FGPPESAEISPLKKLLGPTQPPLYRTVTWTEYLRKKAEHFNNALSSVR 355
+GPP ISPL KL+ P Q PLY ++TW +YL KA HFN +LS +R
Sbjct: 305 WGPPSDIMISPLPKLVDPLQSPLYPSLTWKQYLATKATHFNQSLSIIR 346
BLAST of MELO3C023378 vs. TAIR10
Match:
AT1G80330.1 (AT1G80330.1 gibberellin 3-oxidase 4)
HSP 1 Score: 356.3 bits (913), Expect = 2.3e-98
Identity = 177/339 (52.21%), Postives = 233/339 (68.73%), Query Frame = 1
Query: 28 SLQELPDSYDW--IQPDSF--PNSSLSNNHLSDSIPLIDLSLPNAPHLIGNAFRTWGAFQ 87
SLQ LP+S+ W DS P+S++S + + +SIP+IDLS P+ LIG+A +TWGAFQ
Sbjct: 15 SLQTLPESFTWKLTAADSLLRPSSAVSFDAVEESIPVIDLSNPDVTTLIGDASKTWGAFQ 74
Query: 88 VINHGVPISLLHSIESSANSLFSLPAPHKLKAARPSDGISGYGLVRISSFFPKRMWSEGF 147
+ NHG+ LL IES + +LF +P+ KL+AA G+SGYG RIS FF K+MWSEGF
Sbjct: 75 IANHGISQKLLDDIESLSKTLFDMPSERKLEAASSDKGVSGYGEPRISPFFEKKMWSEGF 134
Query: 148 TIVG-SPLEHFRKLWPHDYTRYCDIVEEYDQEMKSLCGRLMWVALGELGITREDVNWAGP 207
TI S HF LWPHD+T+YC I++EY EM+ L RL++ LG LG+T ED+ WA
Sbjct: 135 TIADDSYRNHFNTLWPHDHTKYCGIIQEYVDEMEKLASRLLYCILGSLGVTVEDIEWAHK 194
Query: 208 --NGDFMTSNAATQFNSYPVCPDPERAMGLGAHTDTSLLTIVYQNNTRGLQVLREGNRWV 267
A + N YPVCP+PERAMGL AHTD+++LTI++Q+NT GLQV RE + WV
Sbjct: 195 LEKSGSKVGRGAIRLNHYPVCPEPERAMGLAAHTDSTILTILHQSNTGGLQVFREESGWV 254
Query: 268 TVEPVPGALVVQVGDLLHILTNGLYPSPVHQAVVNRTRKRLSVAYVF-GPPESAEISPLK 327
TVEP PG LVV +GDL HIL+NG PS VH+A VN TR R+S+AY++ GP +I+P+
Sbjct: 255 TVEPAPGVLVVNIGDLFHILSNGKIPSVVHRAKVNHTRSRISIAYLWGGPAGDVQIAPIS 314
Query: 328 KLLGPTQPPLYRTVTWTEYLRKKAEHFNNALSSVRLCAP 359
KL GP +P LYR++TW EYL+ K E F+ A+ ++R+ P
Sbjct: 315 KLTGPAEPSLYRSITWKEYLQIKYEVFDKAMDAIRVVNP 353
BLAST of MELO3C023378 vs. TAIR10
Match:
AT4G21690.1 (AT4G21690.1 gibberellin 3-oxidase 3)
HSP 1 Score: 306.6 bits (784), Expect = 2.1e-83
Identity = 159/355 (44.79%), Postives = 221/355 (62.25%), Query Frame = 1
Query: 7 ITEVFKSHPVHIPIHKNFDFDSLQELPDSYDWIQPDSFPNSSLSNNHLSDSIPLIDLSLP 66
+T++FK++PV+ DF + + LPDS+ W +P+ P ++ S IP+I LS P
Sbjct: 4 VTQLFKNNPVNRDRIIPLDFTNTKTLPDSHVWSKPE--PETT------SGPIPVISLSNP 63
Query: 67 NAPHLIGNAFRTWGAFQVINHGVPISLLHSIESSANSLFSLPAPHKLKAARPSDGISGYG 126
L+ A WG F + +HGV SLLH+++ LFSLP K+ A R D +GYG
Sbjct: 64 EEHGLLRQACEEWGVFHITDHGVSHSLLHNVDCQMKRLFSLPMHRKILAVRSPDESTGYG 123
Query: 127 LVRISSFFPKRMWSEGFTIVGSPL-EHFRKLWPHDYTRYCDIVEEYDQEMKSLCGRLMWV 186
+VRIS F+ K MWSEGF+++GS L H LWP D+ +C+++EEY + M L RL+ +
Sbjct: 124 VVRISMFYDKLMWSEGFSVMGSSLRRHATLLWPDDHAEFCNVMEEYQKAMDDLSHRLISM 183
Query: 187 ALGELGITREDVNWAGPNGDFMTSNAATQF---NSYPVCPDPERAMGLGAHTDTSLLTIV 246
+G LG+T ED+ W P+ +++ F NSYPVCPDP AMGL HTD+SLLTI+
Sbjct: 184 LMGSLGLTHEDLGWLVPDKTGSGTDSIQSFLQLNSYPVCPDPHLAMGLAPHTDSSLLTIL 243
Query: 247 YQNNTRGLQV---LREGNRWVTVEPVPGALVVQVGDLLHILTNGLYPSPVHQAVVNRTRK 306
YQ N GL++ EG+RW+ VEP+ G+LVV +GDL HI++NG + S +H+AVVN+T
Sbjct: 244 YQGNIPGLEIESPQEEGSRWIGVEPIEGSLVVIMGDLSHIISNGQFRSTMHRAVVNKTHH 303
Query: 307 RLSVAYVFGPPESAEISPLKKLLGPTQPPLYRTVTWTEYLRKKAEHFNNALSSVR 355
R+S AY GPP++ +I PL PP+YR + W EYL KA HFN AL+ R
Sbjct: 304 RVSAAYFAGPPKNLQIGPLTS--DKNHPPIYRRLIWEEYLAAKATHFNKALTLFR 348
BLAST of MELO3C023378 vs. TAIR10
Match:
AT3G21420.1 (AT3G21420.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)
HSP 1 Score: 177.2 bits (448), Expect = 1.9e-44
Identity = 107/305 (35.08%), Postives = 154/305 (50.49%), Query Frame = 1
Query: 47 SSLSNNHLSDSIPLIDLSLPNAPH---------LIGNAFRTWGAFQVINHGVPISLLHSI 106
SSL +HL IP+IDLS + P + A WG FQVINHG+ + ++ I
Sbjct: 44 SSLKTHHLHHQIPVIDLSKLSKPDNDDFFFEILKLSQACEDWGFFQVINHGIEVEVVEDI 103
Query: 107 ESSANSLFSLPAPHKLKAARPSDGISGYGLVRISSFFPKRMWSEGFTI-VGSPLEHFRKL 166
E A+ F +P K K + GYG I S K W F + V P KL
Sbjct: 104 EEVASEFFDMPLEEKKKYPMEPGTVQGYGQAFIFSEDQKLDWCNMFALGVHPPQIRNPKL 163
Query: 167 WPHDYTRYCDIVEEYDQEMKSLCGRLMWVALGELGITREDVNWAGPNGDFMTSNAATQFN 226
WP R+ + +E Y +E++ LC RL+ LG+ E F + A + N
Sbjct: 164 WPSKPARFSESLEGYSKEIRELCKRLLKYIAISLGLKEERFEEM-----FGEAVQAVRMN 223
Query: 227 SYPVCPDPERAMGLGAHTDTSLLTIVYQ--NNTRGLQVLREGNRWVTVEPVPGALVVQVG 286
YP C P+ +GL H+D S LT++ Q N+ GLQ+L++ N WV V+P+P ALV+ +G
Sbjct: 224 YYPPCSSPDLVLGLSPHSDGSALTVLQQSKNSCVGLQILKD-NTWVPVKPLPNALVINIG 283
Query: 287 DLLHILTNGLYPSPVHQAVVNRTRKRLSVAYVFGPPESAEISPLKKLL-GPTQPPLYRTV 339
D + +L+NG Y S H+AV NR ++RL++ + P EI P+ +L+ T P YR+
Sbjct: 284 DTIEVLSNGKYKSVEHRAVTNREKERLTIVTFYAPNYEVEIEPMSELVDDETNPCKYRSY 342
BLAST of MELO3C023378 vs. NCBI nr
Match:
gi|659122279|ref|XP_008461056.1| (PREDICTED: gibberellin 3-beta-dioxygenase 1-like [Cucumis melo])
HSP 1 Score: 774.2 bits (1998), Expect = 1.0e-220
Identity = 374/374 (100.00%), Postives = 374/374 (100.00%), Query Frame = 1
Query: 1 MGSNIKITEVFKSHPVHIPIHKNFDFDSLQELPDSYDWIQPDSFPNSSLSNNHLSDSIPL 60
MGSNIKITEVFKSHPVHIPIHKNFDFDSLQELPDSYDWIQPDSFPNSSLSNNHLSDSIPL
Sbjct: 1 MGSNIKITEVFKSHPVHIPIHKNFDFDSLQELPDSYDWIQPDSFPNSSLSNNHLSDSIPL 60
Query: 61 IDLSLPNAPHLIGNAFRTWGAFQVINHGVPISLLHSIESSANSLFSLPAPHKLKAARPSD 120
IDLSLPNAPHLIGNAFRTWGAFQVINHGVPISLLHSIESSANSLFSLPAPHKLKAARPSD
Sbjct: 61 IDLSLPNAPHLIGNAFRTWGAFQVINHGVPISLLHSIESSANSLFSLPAPHKLKAARPSD 120
Query: 121 GISGYGLVRISSFFPKRMWSEGFTIVGSPLEHFRKLWPHDYTRYCDIVEEYDQEMKSLCG 180
GISGYGLVRISSFFPKRMWSEGFTIVGSPLEHFRKLWPHDYTRYCDIVEEYDQEMKSLCG
Sbjct: 121 GISGYGLVRISSFFPKRMWSEGFTIVGSPLEHFRKLWPHDYTRYCDIVEEYDQEMKSLCG 180
Query: 181 RLMWVALGELGITREDVNWAGPNGDFMTSNAATQFNSYPVCPDPERAMGLGAHTDTSLLT 240
RLMWVALGELGITREDVNWAGPNGDFMTSNAATQFNSYPVCPDPERAMGLGAHTDTSLLT
Sbjct: 181 RLMWVALGELGITREDVNWAGPNGDFMTSNAATQFNSYPVCPDPERAMGLGAHTDTSLLT 240
Query: 241 IVYQNNTRGLQVLREGNRWVTVEPVPGALVVQVGDLLHILTNGLYPSPVHQAVVNRTRKR 300
IVYQNNTRGLQVLREGNRWVTVEPVPGALVVQVGDLLHILTNGLYPSPVHQAVVNRTRKR
Sbjct: 241 IVYQNNTRGLQVLREGNRWVTVEPVPGALVVQVGDLLHILTNGLYPSPVHQAVVNRTRKR 300
Query: 301 LSVAYVFGPPESAEISPLKKLLGPTQPPLYRTVTWTEYLRKKAEHFNNALSSVRLCAPLT 360
LSVAYVFGPPESAEISPLKKLLGPTQPPLYRTVTWTEYLRKKAEHFNNALSSVRLCAPLT
Sbjct: 301 LSVAYVFGPPESAEISPLKKLLGPTQPPLYRTVTWTEYLRKKAEHFNNALSSVRLCAPLT 360
Query: 361 GLLDVNDHSQVKVG 375
GLLDVNDHSQVKVG
Sbjct: 361 GLLDVNDHSQVKVG 374
BLAST of MELO3C023378 vs. NCBI nr
Match:
gi|449436096|ref|XP_004135830.1| (PREDICTED: gibberellin 3-beta-dioxygenase 1 [Cucumis sativus])
HSP 1 Score: 755.7 bits (1950), Expect = 3.7e-215
Identity = 365/374 (97.59%), Postives = 368/374 (98.40%), Query Frame = 1
Query: 1 MGSNIKITEVFKSHPVHIPIHKNFDFDSLQELPDSYDWIQPDSFPNSSLSNNHLSDSIPL 60
MGSNIKITEVFKSHPVHIPIHKN DFDSL ELPDSYDWIQPDSFP+SSLSNNHLSDSIPL
Sbjct: 1 MGSNIKITEVFKSHPVHIPIHKNLDFDSLHELPDSYDWIQPDSFPSSSLSNNHLSDSIPL 60
Query: 61 IDLSLPNAPHLIGNAFRTWGAFQVINHGVPISLLHSIESSANSLFSLPAPHKLKAARPSD 120
IDLSLPNAP LIGNAFRTWGAFQVINHGVPISLLHSIESSANSLFSLP PHKLKAARPSD
Sbjct: 61 IDLSLPNAPQLIGNAFRTWGAFQVINHGVPISLLHSIESSANSLFSLPPPHKLKAARPSD 120
Query: 121 GISGYGLVRISSFFPKRMWSEGFTIVGSPLEHFRKLWPHDYTRYCDIVEEYDQEMKSLCG 180
GISGYGLVRISSFFPKRMWSEGFTIVGSPLEHFRKLWPHDYTRYCDIVEEYD+EMKSLCG
Sbjct: 121 GISGYGLVRISSFFPKRMWSEGFTIVGSPLEHFRKLWPHDYTRYCDIVEEYDREMKSLCG 180
Query: 181 RLMWVALGELGITREDVNWAGPNGDFMTSNAATQFNSYPVCPDPERAMGLGAHTDTSLLT 240
RLMWVALGELGITREDVNWAGPNGDF TSNAATQ NSYPVCPDP+RAMGLGAHTDTSLLT
Sbjct: 181 RLMWVALGELGITREDVNWAGPNGDFKTSNAATQLNSYPVCPDPDRAMGLGAHTDTSLLT 240
Query: 241 IVYQNNTRGLQVLREGNRWVTVEPVPGALVVQVGDLLHILTNGLYPSPVHQAVVNRTRKR 300
IVYQNNTRGLQVLREGNRWVTVEPVPGALVVQVGDLLHILTNGLYPSPVHQAVVNRTRKR
Sbjct: 241 IVYQNNTRGLQVLREGNRWVTVEPVPGALVVQVGDLLHILTNGLYPSPVHQAVVNRTRKR 300
Query: 301 LSVAYVFGPPESAEISPLKKLLGPTQPPLYRTVTWTEYLRKKAEHFNNALSSVRLCAPLT 360
LSVAYVFGPPESAEISPLKKLLGPTQPPLYRTVTWTEYLRKKAEHFNNALSSVRLCAPLT
Sbjct: 301 LSVAYVFGPPESAEISPLKKLLGPTQPPLYRTVTWTEYLRKKAEHFNNALSSVRLCAPLT 360
Query: 361 GLLDVNDHSQVKVG 375
GLLDVNDHSQVKVG
Sbjct: 361 GLLDVNDHSQVKVG 374
BLAST of MELO3C023378 vs. NCBI nr
Match:
gi|27123661|emb|CAC83089.1| (gibberellin 3-oxidase [Cucurbita maxima])
HSP 1 Score: 645.6 bits (1664), Expect = 5.4e-182
Identity = 312/373 (83.65%), Postives = 339/373 (90.88%), Query Frame = 1
Query: 7 ITEVFKSHPVHIPIHKNFDFDSLQELPDSYDWIQPDSFPNSSLSNNHL-----SDSIPLI 66
I +VFKS PVHIP HKN DFDSL ELPDSY WIQPDSFP+ + +++ SDS+PLI
Sbjct: 5 IADVFKSFPVHIPAHKNLDFDSLHELPDSYAWIQPDSFPSPTHKHHNSILDSDSDSVPLI 64
Query: 67 DLSLPNAPHLIGNAFRTWGAFQVINHGVPISLLHSIESSANSLFSLPAPHKLKAARPSDG 126
DLSLPNA LIGNAFR+WGAFQVINHGVPISLL SIESSA++LFSLP HKLKAAR DG
Sbjct: 65 DLSLPNAAALIGNAFRSWGAFQVINHGVPISLLQSIESSADTLFSLPPSHKLKAARTPDG 124
Query: 127 ISGYGLVRISSFFPKRMWSEGFTIVGSPLEHFRKLWPHDYTRYCDIVEEYDQEMKSLCGR 186
ISGYGLVRISSFFPKRMWSEGFTIVGSPL+HFR+LWPHDY ++C+IVEEYD+EM+SLCGR
Sbjct: 125 ISGYGLVRISSFFPKRMWSEGFTIVGSPLDHFRQLWPHDYHKHCEIVEEYDREMRSLCGR 184
Query: 187 LMWVALGELGITREDVNWAGPNGDFMTSNAATQFNSYPVCPDPERAMGLGAHTDTSLLTI 246
LMW+ LGELGITR+D+ WAGP+GDF TS AATQFNSYPVCPDP+RAMGLG HTDTSLLTI
Sbjct: 185 LMWLGLGELGITRDDMKWAGPDGDFKTSPAATQFNSYPVCPDPDRAMGLGPHTDTSLLTI 244
Query: 247 VYQNNTRGLQVLREGNRWVTVEPVPGALVVQVGDLLHILTNGLYPSPVHQAVVNRTRKRL 306
VYQ+NTRGLQVLREG RWVTVEPV G LVVQVGDLLHILTNGLYPS +HQAVVNRTRKRL
Sbjct: 245 VYQSNTRGLQVLREGKRWVTVEPVAGGLVVQVGDLLHILTNGLYPSALHQAVVNRTRKRL 304
Query: 307 SVAYVFGPPESAEISPLKKLLGPTQPPLYRTVTWTEYLRKKAEHFNNALSSVRLCAPLTG 366
SVAYVFGPPESAEISPLKKLLGPTQPPLYR VTWTEYL KKAEHFNNALS+VRLCAP+TG
Sbjct: 305 SVAYVFGPPESAEISPLKKLLGPTQPPLYRPVTWTEYLGKKAEHFNNALSTVRLCAPITG 364
Query: 367 LLDVNDHSQVKVG 375
LLDVNDHS+VKVG
Sbjct: 365 LLDVNDHSRVKVG 377
BLAST of MELO3C023378 vs. NCBI nr
Match:
gi|525507174|ref|NP_001267625.1| (gibberellin 3-beta-dioxygenase 1-like [Cucumis sativus])
HSP 1 Score: 604.7 bits (1558), Expect = 1.1e-169
Identity = 286/368 (77.72%), Postives = 326/368 (88.59%), Query Frame = 1
Query: 6 KITEVFKSHPVHIPIHKNFDFDSLQELPDSYDWIQPDSFPNSSLSNNHLSDSIPLIDLSL 65
K+T+++K+HP+ IP +K + DSLQ+LPD+YDWIQPD+FP+ N LSDS+PLIDLSL
Sbjct: 8 KLTDIYKTHPLDIPTNK-LNLDSLQQLPDTYDWIQPDAFPSFRDPNISLSDSVPLIDLSL 67
Query: 66 PNAPHLIGNAFRTWGAFQVINHGVPISLLHSIESSANSLFSLPAPHKLKAARPSDGISGY 125
PNAPHLIGNA +TWG FQVINHGVPISLL+S+ES N+LF LP+PHKLKAAR DG++GY
Sbjct: 68 PNAPHLIGNALKTWGVFQVINHGVPISLLNSMESFTNTLFDLPSPHKLKAARTPDGVTGY 127
Query: 126 GLVRISSFFPKRMWSEGFTIVGSPLEHFRKLWPHDYTRYCDIVEEYDQEMKSLCGRLMWV 185
GL RIS+FFPKRMWSEGFTIVGSPLEHF+KLWP++Y++YCDI EEY +EMKSLCGR++W+
Sbjct: 128 GLPRISTFFPKRMWSEGFTIVGSPLEHFQKLWPNEYSKYCDITEEYSREMKSLCGRVIWL 187
Query: 186 ALGELGITREDVNWAGPNGDFMTSNAATQFNSYPVCPDPERAMGLGAHTDTSLLTIVYQN 245
ALGELGITREDVNWA NGDF TSNA Q NSYPVCPDP+RAMGLGAHTDTSLLTI+YQN
Sbjct: 188 ALGELGITREDVNWAEANGDFKTSNAVIQLNSYPVCPDPDRAMGLGAHTDTSLLTILYQN 247
Query: 246 NTRGLQVLREGNRWVTVEPVPGALVVQVGDLLHILTNGLYPSPVHQAVVNRTRKRLSVAY 305
NTRGLQVLREGNRWV VEPV GALVVQVGDLLHILTNGLYPS HQAVVN+ RKR+S+AY
Sbjct: 248 NTRGLQVLREGNRWVMVEPVTGALVVQVGDLLHILTNGLYPSSAHQAVVNQNRKRISIAY 307
Query: 306 VFGPPESAEISPLKKLLGPTQPPLYRTVTWTEYLRKKAEHFNNALSSVRLCAPLTGLLDV 365
FGP ESAEISPL KL+ PTQP LY TVTWTEYLRKKA+ FNN LSS+RL APLTG LD+
Sbjct: 308 FFGPSESAEISPLNKLVTPTQPLLYPTVTWTEYLRKKAQLFNNTLSSIRLSAPLTGALDI 367
Query: 366 NDHSQVKV 374
NDH+QVKV
Sbjct: 368 NDHNQVKV 374
BLAST of MELO3C023378 vs. NCBI nr
Match:
gi|304636273|gb|ADM47338.1| (gibberellin 3-oxidase 2 [Marah macrocarpus])
HSP 1 Score: 590.9 bits (1522), Expect = 1.6e-165
Identity = 281/370 (75.95%), Postives = 318/370 (85.95%), Query Frame = 1
Query: 6 KITEVFKSHPVHIPIHKNFDFDSLQELPDSYDWIQPDSFPNSSLSNNHLSDSIPLIDLSL 65
KIT+ FKSHPVH+P HKN DFDS ELPD+Y+W+QPDS P+ N+ + +PLIDLSL
Sbjct: 4 KITDAFKSHPVHVPAHKNLDFDSFNELPDTYEWVQPDSIPSP---NDQSQEHLPLIDLSL 63
Query: 66 PNAPHLIGNAFRTWGAFQVINHGVPISLLHSIESSANSLFSLPAPHKLKAARPSDGISGY 125
PNA L+G+AFR+WGAFQ INHGVPISLLHS ES+ ++LFSLP K KAAR DG+ GY
Sbjct: 64 PNAAALVGDAFRSWGAFQAINHGVPISLLHSFESTVDTLFSLPPSDKHKAARTPDGVDGY 123
Query: 126 GLVRISSFFPKRMWSEGFTIVGSPLEHFRKLWPHDYTRYCDIVEEYDQEMKSLCGRLMWV 185
GLVRISSFFPKRMWSEGFTI+ SPL HF+KLWP DY ++C+I EEYD+EM+SLC RL+W+
Sbjct: 124 GLVRISSFFPKRMWSEGFTILNSPLHHFQKLWPDDYAKHCEIYEEYDREMRSLCARLIWL 183
Query: 186 ALGELGITREDVNWAGPNGDFMTSNAATQFNSYPVCPDPERAMGLGAHTDTSLLTIVYQN 245
LGELGIT++DV AGP+ DF T+ AATQ NSYP+CPDPERAMGLGAHTDTSLLTIVYQN
Sbjct: 184 GLGELGITQDDVELAGPDRDFKTNPAATQLNSYPICPDPERAMGLGAHTDTSLLTIVYQN 243
Query: 246 NTRGLQVLREGNRWVTVEPVPGALVVQVGDLLHILTNGLYPSPVHQAVVNRTRKRLSVAY 305
NT GLQ LREGNRWVTVEPV GALVVQVGDLL I+TNGLY SP+HQA VNRTRKRLS+AY
Sbjct: 244 NTSGLQFLREGNRWVTVEPVDGALVVQVGDLLQIMTNGLYKSPLHQAFVNRTRKRLSMAY 303
Query: 306 VFGPPESAEISPLKKLLGPTQPPLYRTVTWTEYLRKKAEHFNNALSSVRLCAP-LTGLLD 365
+FG PE+AEISPLKKLLGPTQPP+YRTVTWTEYL KKAEHFNNAL SVR+CAP GLLD
Sbjct: 304 IFGTPETAEISPLKKLLGPTQPPIYRTVTWTEYLGKKAEHFNNALKSVRICAPSANGLLD 363
Query: 366 VNDHSQVKVG 375
VND SQVKVG
Sbjct: 364 VNDQSQVKVG 370
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
G3OX_PEA | 4.5e-128 | 59.52 | Gibberellin 3-beta-dioxygenase 1 OS=Pisum sativum GN=LE PE=1 SV=1 | [more] |
G3OX1_ARATH | 6.1e-117 | 57.88 | Gibberellin 3-beta-dioxygenase 1 OS=Arabidopsis thaliana GN=GA3OX1 PE=1 SV=2 | [more] |
G3OX2_ARATH | 2.8e-114 | 55.75 | Gibberellin 3-beta-dioxygenase 2 OS=Arabidopsis thaliana GN=GA3OX2 PE=1 SV=2 | [more] |
G3OX4_ARATH | 4.1e-97 | 52.21 | Gibberellin 3-beta-dioxygenase 4 OS=Arabidopsis thaliana GN=GA3OX4 PE=1 SV=1 | [more] |
G3OX3_ARATH | 3.7e-82 | 44.79 | Gibberellin 3-beta-dioxygenase 3 OS=Arabidopsis thaliana GN=GA3OX3 PE=2 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
S0BE27_CUCSA | 7.4e-170 | 77.72 | GA 3-oxidase OS=Cucumis sativus GN=GA3ox3 PE=2 SV=1 | [more] |
E1AXG5_9ROSI | 1.1e-165 | 75.95 | Gibberellin 3-oxidase 2 (Fragment) OS=Marah macrocarpus PE=2 SV=1 | [more] |
A0A0A0KAA7_CUCSA | 5.9e-151 | 69.60 | Uncharacterized protein OS=Cucumis sativus GN=Csa_7G435470 PE=3 SV=1 | [more] |
M5XCD8_PRUPE | 1.8e-147 | 68.11 | Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa007336mg PE=3 SV=1 | [more] |
Q9M4P2_CUCMA | 1.1e-144 | 68.14 | Gibberellin 3-beta-hydroxylase OS=Cucurbita maxima PE=2 SV=1 | [more] |