MELO3C023254 (gene) Melon (DHL92) v3.5.1

BLAST of MELO3C023254 vs. Swiss-Prot
Match: PME51_ARATH (Probable pectinesterase/pectinesterase inhibitor 51 OS=Arabidopsis thaliana GN=PME51 PE=2 SV=1)

HSP 1 Score: 726.5 bits (1874), Expect = 2.3e-208
Identity = 362/551 (65.70%), Postives = 426/551 (77.31%), Query Frame = 1

Query: 11  SLSFFFFSLSL-SSSSSAVRHHHSHSHLPSHFTNTPSD---QILHACKSTRFPDQCGSSL 70
           S+    FSL L SS SS+ RHHH H+   +   N  S    QI  AC +TR+PDQC SSL
Sbjct: 3   SILILLFSLFLFSSPSSSSRHHHHHNSGDTSPVNPSSSLAAQIRLACNATRYPDQCVSSL 62

Query: 71  SHSSLKPPQNNPSPLQILQSAIALSSDGLTTAQSLVKQILDSSATNLNRTTAAKNCLEVL 130
           S     PP  +P P+QI+ SAI+ S   L TAQS +K I+DSS  NLNRT AA  CL++L
Sbjct: 63  SEQGRVPP--DPKPIQIIHSAISFSFQNLKTAQSKIKSIVDSSVGNLNRTNAANTCLQLL 122

Query: 131 RNSDYRISLTNDALHRGRTKDARAWLSASLLYHYDCWSALKYANDTQLVDKTMAFLDSLI 190
             S++R   T+ AL RG+ KDARAW+SA+L+Y YD WSALKY NDT  V +TM+FLD LI
Sbjct: 123 TYSEHRTQSTDQALTRGKIKDARAWMSAALVYQYDSWSALKYVNDTSQVGETMSFLDGLI 182

Query: 191 GLSSNALSMLVAYDIYGNDTAAWTPPRTERDGFWEGGGGGSGL----GFSGGFPAKVTAD 250
            ++SNALSM+V+YD +G++ A+WT P TERDGFWE  G G GL    G + GFP+ +  D
Sbjct: 183 HVTSNALSMMVSYDNFGDNVASWTYPATERDGFWEKTGPGLGLDPSTGLNLGFPSGLKED 242

Query: 251 ATVCKDGSGGCYGSIQKAVDAAPENAAARRFVIHIKEGVYEEIVRVPLEKKNVVFLGDGM 310
            TVCKDG  G Y ++Q AV+AAPE+   R+FVI I EGVYEE V VP EKKNVVF+GDGM
Sbjct: 243 VTVCKDGKCG-YKTVQDAVNAAPEDNGMRKFVIKISEGVYEENVIVPFEKKNVVFIGDGM 302

Query: 311 GKTVITGSLNAGQPGISTYNTATLGVLGDGFMASGLTIQNTAGPDAHQAVAFRSDSDLSV 370
           GKTVITGSLNAG PGI+TYNTAT+GV+GDGFMA  LT QNTAGPDAHQAVAFRSDSD S+
Sbjct: 303 GKTVITGSLNAGMPGITTYNTATVGVVGDGFMARDLTFQNTAGPDAHQAVAFRSDSDFSL 362

Query: 371 IQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQDCEILIRPRQLKPEKGE 430
           I++CEF+GNQDTLYAH LRQFYK+C+IQGNVDFIFGNSA++FQDCEILI PRQ+ PEKGE
Sbjct: 363 IENCEFLGNQDTLYAHGLRQFYKNCRIQGNVDFIFGNSAAVFQDCEILIAPRQINPEKGE 422

Query: 431 NNAITAHGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKVHKNFLGRPWKEYSRTVFIH 490
            NA+TA GR DP+QSTGFVF NCLINGT+EYM L+ + PKVHKNFLGRPWK+YSRTVFI 
Sbjct: 423 KNAVTAQGRIDPSQSTGFVFLNCLINGTEEYMKLFKANPKVHKNFLGRPWKDYSRTVFIG 482

Query: 491 CNLEALVSPSGWMPWSGDFALKTLFYGEFENSGVGSKTSERVGWSSQIPAQHVFSYSVQN 550
           CNLEAL++P GW+PWSGDFALKTL+YGE +N+G GS  S+RV WSSQIP +HV  YSV N
Sbjct: 483 CNLEALITPDGWLPWSGDFALKTLYYGESKNTGPGSDRSQRVSWSSQIPDEHVHVYSVAN 542

Query: 551 FIQGDEWIPSS 554
           FIQ DEW   S
Sbjct: 543 FIQADEWASMS 550

BLAST of MELO3C023254 vs. Swiss-Prot
Match: PME64_ARATH (Probable pectinesterase/pectinesterase inhibitor 64 OS=Arabidopsis thaliana GN=PME64 PE=2 SV=2)

HSP 1 Score: 656.0 bits (1691), Expect = 3.8e-187
Identity = 327/551 (59.35%), Postives = 405/551 (73.50%), Query Frame = 1

Query: 19  LSLSSSSSAVRHHHSHSHLPSHFTNTPSD--------------QILHACKSTRFPDQCGS 78
           L  ++ S + R+HH+    PSH T T  D              QI  AC +TRFPD C +
Sbjct: 53  LIYATVSKSSRNHHN----PSHQTPTSDDHPPPETPPSPPPIAQIRLACNATRFPDHCVA 112

Query: 79  SLSHSSLKPPQNNPSPLQILQSAIALSSDGLTTAQSLVKQILDSSATNLNRTTAAKNCLE 138
           SLS     PP  +P P+QI+ SAI++S + L + QS ++ ILDSSA N NRT  A  CLE
Sbjct: 113 SLSKPGQVPP--DPKPVQIIHSAISVSYENLKSGQSKIQSILDSSAGNRNRTNIATICLE 172

Query: 139 VLRNSDYRISLTNDALHRGRTKDARAWLSASLLYHYDCWSALKYANDTQLVDKTMAFLDS 198
           +L  S +R   T+ A+  G  KDARAW+SA+L Y +DCWS LK  NDT+ V  T+ F + 
Sbjct: 173 ILSYSQHRTESTDIAVTSGDIKDARAWMSAALAYQFDCWSGLKTVNDTKQVVDTITFFEG 232

Query: 199 LIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGFWEGGG----GGSGLGFSGGFPAKVT 258
           L+ L+ NALSM++++D +G+D  +W  P TERDGFWE  G     G+G   S GFP+ +T
Sbjct: 233 LVNLTGNALSMMLSFDSFGDDVVSWIRPATERDGFWEKAGPSLGSGTGTEASLGFPSGLT 292

Query: 259 ADATVCKDGSGGC-YGSIQKAVDAAPENAAARRFVIHIKEGVYEEIVRVPLEKKNVVFLG 318
            D TVCK+G   C Y ++Q+AVD+AP+     +FVI I+EGVYEE VRVP EKKNVVF+G
Sbjct: 293 EDVTVCKNGGKDCKYKTVQEAVDSAPDTNRTVKFVIRIREGVYEETVRVPFEKKNVVFIG 352

Query: 319 DGMGKTVITGSLNAGQPGISTYNTATLGVLGDGFMASGLTIQNTAGPDAHQAVAFRSDSD 378
           DGMGKTVITGSLN GQPG++T+ +AT+GVLGDGFMA  LTI+NTAG DAHQAVAFRSDSD
Sbjct: 353 DGMGKTVITGSLNVGQPGMTTFESATVGVLGDGFMARDLTIENTAGADAHQAVAFRSDSD 412

Query: 379 LSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQDCEILIRPRQLKPE 438
            SV+++CEF+GNQDTLYAHSLRQFYK C+IQGNVDFIFGNSA++FQDC+ILI  +  K E
Sbjct: 413 FSVLENCEFLGNQDTLYAHSLRQFYKQCRIQGNVDFIFGNSAAVFQDCDILIASKHSKLE 472

Query: 439 K-GENNAITAHGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKVHKNFLGRPWKEYSRT 498
           + G NNAITAHGR D +QSTGFVF NC INGT+EYM  + + P+ HKNFLGRPWKE+SRT
Sbjct: 473 QGGANNAITAHGRIDASQSTGFVFLNCSINGTEEYMKEFQANPEGHKNFLGRPWKEFSRT 532

Query: 499 VFIHCNLEALVSPSGWMPWSGDFALKTLFYGEFENSGVGSKTSERVGWSSQIPAQHVFSY 550
           VF++CNLE+L+SP GWMPW+GDFALKTL+YGE++N+G GS  S RV WSS+IP +HV  Y
Sbjct: 533 VFVNCNLESLISPDGWMPWNGDFALKTLYYGEYKNTGPGSVRSSRVPWSSEIPEKHVDVY 592

BLAST of MELO3C023254 vs. Swiss-Prot
Match: PME2_CITSI (Pectinesterase 2 OS=Citrus sinensis GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 310.8 bits (795), Expect = 3.0e-83
Identity = 192/473 (40.59%), Postives = 269/473 (56.87%), Query Frame = 1

Query: 87  AIALSSDGLTTAQSLVKQILDSSATNLNRTTAAKNCLEVLRNSDYRISLTNDALHRGRTK 146
           ++ L+ +  TTAQS     L S   N     A ++C E+   +  +++ T+++       
Sbjct: 64  SLQLALERATTAQSRT-YTLGSKCRNEREKAAWEDCRELYELTVLKLNQTSNSSPGCTKV 123

Query: 147 DARAWLSASLLYHYDCWSALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDIYGNDT 206
           D + WLS +L     C ++L+           +   + ++ L SN ++ L++  +  N  
Sbjct: 124 DKQTWLSTALTNLETCRASLE----------DLGVPEYVLPLLSNNVTKLISNTLSLNKV 183

Query: 207 AAWTPPRTERDGF--WEGGGGGSGLGFSGGFPAKVTADATVCKDGSGGCYGSIQKAVDAA 266
               P  + +DGF  W   G    L           A+  V +DGSG    +IQ+AV AA
Sbjct: 184 PYNEP--SYKDGFPTWVKPGDRKLL------QTTPRANIVVAQDGSGNVK-TIQEAV-AA 243

Query: 267 PENAAARRFVIHIKEGVYEEIVRVPLEKKNVVFLGDGMGKTVITGSLNAGQPGISTYNTA 326
              A   R+VI+IK G Y E + V L  KN++F+GDG+GKT+ITGS + G  G +T+ +A
Sbjct: 244 ASRAGGSRYVIYIKAGTYNENIEVKL--KNIMFVGDGIGKTIITGSKSVGG-GATTFKSA 303

Query: 327 TLGVLGDGFMASGLTIQNTAGPDAHQAVAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFY 386
           T+ V+GD F+A  +TI+NTAGP+ HQAVA RS SDLSV   C F G QDTLY HS RQFY
Sbjct: 304 TVAVVGDNFIARDITIRNTAGPNNHQAVALRSGSDLSVFYRCSFEGYQDTLYVHSQRQFY 363

Query: 387 KSCKIQGNVDFIFGNSASIFQDCEILIRPRQLKPEKGENNAITAHGRTDPAQSTGFVFQN 446
           + C I G VDFIFGN+A + Q+C I  R    KP     N +TA GRTDP QSTG +  N
Sbjct: 364 RECDIYGTVDFIFGNAAVVLQNCNIFAR----KP-PNRTNTLTAQGRTDPNQSTGIIIHN 423

Query: 447 CLINGTQEYMSLYYSKPKVHKNFLGRPWKEYSRTVFIHCNLEALVSPSGWMPWSGDFALK 506
           C +    +   +  S     K FLGRPWK+YSRTV+I   L++L++P+GWM WSGDFAL 
Sbjct: 424 CRVTAASDLKPVQSSV----KTFLGRPWKQYSRTVYIKTFLDSLINPAGWMEWSGDFALN 483

Query: 507 TLFYGEFENSGVGSKTSERVGWSS----QIPAQHVFSYSVQNFIQGDEWIPSS 554
           TL+Y E+ N+G GS T+ RV W        P+Q V  ++V NFI G+ W+P++
Sbjct: 484 TLYYAEYMNTGPGSSTANRVKWRGYHVLTSPSQ-VSQFTVGNFIAGNSWLPAT 502

BLAST of MELO3C023254 vs. Swiss-Prot
Match: PME17_ARATH (Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana GN=PME17 PE=2 SV=2)

HSP 1 Score: 308.5 bits (789), Expect = 1.5e-82
Identity = 191/505 (37.82%), Postives = 271/505 (53.66%), Query Frame = 1

Query: 53  CKSTRFPDQCGSSLSHSSLKPPQNNPSPLQILQSAIALSSDGLTTAQSLVKQILDSSATN 112
           C  T  P  C   L+H+S   P  + S    +   + L    L    +     L     +
Sbjct: 34  CSQTPNPKPCEYFLTHNSNNEPIKSESEFLKISMKLVLDRAILAKTHAFT---LGPKCRD 93

Query: 113 LNRTTAAKNCLEVLRNSDYRISLTNDALHRGRTKDARAWLSASLLYHYDCWSALKYANDT 172
                A ++C+++   +  +I+ T D   +    DA+ WLS +L     C +       T
Sbjct: 94  TREKAAWEDCIKLYDLTVSKINETMDPNVKCSKLDAQTWLSTALTNLDTCRAGFLELGVT 153

Query: 173 QLVDKTMAFLDSLIGLSSNALSMLVAYDIYGNDTAA-WTPPRTERDGFWEGGGGGSGLGF 232
            +V          + L SN +S L+   +  N     +TPP  E+DGF      G     
Sbjct: 154 DIV----------LPLMSNNVSNLLCNTLAINKVPFNYTPP--EKDGFPSWVKPGDRKLL 213

Query: 233 SGGFPAKVTADATVCKDGSGGCYGSIQKAVDAAPENAAARRFVIHIKEGVYEEIVRVPLE 292
               P     +A V KDGSG  + +I++A+DAA   + + RFVI++K+GVY E   + + 
Sbjct: 214 QSSTPKD---NAVVAKDGSGN-FKTIKEAIDAA---SGSGRFVIYVKQGVYSE--NLEIR 273

Query: 293 KKNVVFLGDGMGKTVITGSLNAGQPGISTYNTATLGVLGDGFMASGLTIQNTAGPDAHQA 352
           KKNV+  GDG+GKT+ITGS + G  G +T+N+AT+  +GDGF+A G+T +NTAG    QA
Sbjct: 274 KKNVMLRGDGIGKTIITGSKSVGG-GTTTFNSATVAAVGDGFIARGITFRNTAGASNEQA 333

Query: 353 VAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQDCEILI 412
           VA RS SDLSV   C F   QDTLY HS RQFY+ C + G VDFIFGN+A++ Q+C I  
Sbjct: 334 VALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAVLQNCNIFA 393

Query: 413 RPRQLKPEKGENNAITAHGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKVHKNFLGRP 472
           R    +P + + N ITA GR+DP Q+TG +  N  +    +   +  S     K +LGRP
Sbjct: 394 R----RP-RSKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGST----KTYLGRP 453

Query: 473 WKEYSRTVFIHCNLEALVSPSGWMPWSGDFALKTLFYGEFENSGVGSKTSERVGWSS--- 532
           W++YSRTVF+  +L++L+ P GW+ W G+FALKTLFY EF+N+G G+ TS RV W     
Sbjct: 454 WRQYSRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGFRV 504

Query: 533 QIPAQHVFSYSVQNFIQGDEWIPSS 554
              A     ++V  F+ G  WIPSS
Sbjct: 514 LGSASEASKFTVGTFLAGGSWIPSS 504

BLAST of MELO3C023254 vs. Swiss-Prot
Match: PME23_ARATH (Probable pectinesterase/pectinesterase inhibitor 23 OS=Arabidopsis thaliana GN=PME23 PE=2 SV=3)

HSP 1 Score: 307.4 bits (786), Expect = 3.3e-82
Identity = 192/559 (34.35%), Postives = 288/559 (51.52%), Query Frame = 1

Query: 17  FSLSLSSSSSAVRHHHSHSHLPSHFTNTPSDQILHACKSTRFPDQCGSSLSHSSLKPPQN 76
           F + +  S + V   HS     +H   T +  +   C  T F D C +SL  +S  P  +
Sbjct: 20  FLVIMVVSVAVVTSKHSPKDDENHIRKT-TKAVQAVCAPTDFKDTCVNSLMGAS--PDSD 79

Query: 77  NPSPLQILQSAIALSS--DGLTTAQSLVKQILDSSATNLNRTTAAKNCL-----EVLRNS 136
           +P  L  L   + + S  + L  A   +K   D +          +  +     ++ +  
Sbjct: 80  DPVDLIKLGFKVTIKSINESLEKASGDIKAKADKNPEAKGAFELCEKLMIDAIDDLKKCM 139

Query: 137 DYRISLTNDALHRGRTKDARAWLSASLLYHYDCWSALKYANDTQLVDKTMAFLDSLIGLS 196
           D+  S+    +     +D R WLS S+ +   C  +        + D    F  S   LS
Sbjct: 140 DHGFSVDQIEVF---VEDLRVWLSGSIAFQQTCMDSFGEIKSNLMQDMLKIFKTSR-ELS 199

Query: 197 SNALSMLVAYD--IYGNDTAAWTPPRTE--------RDGF--WEGGGGGSGLGFSGGFPA 256
           SN+L+M+      I  ++    T    +         D    W G      +   GG P 
Sbjct: 200 SNSLAMVTRISTLIPNSNLTGLTGALAKYARKLLSTEDSIPTWVGPEARRLMAAQGGGPG 259

Query: 257 KVTADATVCKDGSGGCYGSIQKAVDAAPENAAARRFVIHIKEGVYEEIVRVPLEKKNVVF 316
            V A+A V +DG+G  + +I  A++A P+      F+IHIKEG+Y+E V V  +  +V F
Sbjct: 260 PVKANAVVAQDGTGQ-FKTITDALNAVPKGNKVP-FIIHIKEGIYKEKVTVTKKMPHVTF 319

Query: 317 LGDGMGKTVITGSLNAGQPGISTYNTATLGVLGDGFMASGLTIQNTAGPDAHQAVAFRSD 376
           +GDG  KT+ITGSLN G   + T+ TAT+ + GD F A  + I+NTAGP+  QAVA R  
Sbjct: 320 IGDGPNKTLITGSLNFGIGKVKTFLTATITIEGDHFTAKNIGIENTAGPEGGQAVALRVS 379

Query: 377 SDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQDCEILIRPRQLK 436
           +D +V   C+  G+QDTLY HS RQFY+ C + G VDFIFG++  I Q+C+I++R    K
Sbjct: 380 ADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTVSGTVDFIFGDAKCILQNCKIVVR----K 439

Query: 437 PEKGENNAITAHGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKVHKNFLGRPWKEYSR 496
           P KG+   +TA GR++  +STG V   C I G   Y+ +      V+K +LGRPWKE+SR
Sbjct: 440 PNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDPAYIPM----KSVNKAYLGRPWKEFSR 499

Query: 497 TVFIHCNLEALVSPSGWMPWSGDFALKTLFYGEFENSGVGSKTSERVGWS--SQIPAQHV 555
           T+ +   ++ ++ P+GW+PWSGDFALKTL+Y E  N+G GS  ++RV W    ++  Q  
Sbjct: 500 TIIMKTTIDDVIDPAGWLPWSGDFALKTLYYAEHMNTGPGSNQAQRVKWPGIKKLTPQDA 559

BLAST of MELO3C023254 vs. TrEMBL
Match: A0A0A0LQ80_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_2G302130 PE=4 SV=1)

HSP 1 Score: 1101.7 bits (2848), Expect = 0.0e+00
Identity = 546/572 (95.45%), Postives = 556/572 (97.20%), Query Frame = 1

Query: 1   MASLSFLSFLSLSFFFFSLSLSSSSSAVRHHHSHSHLPSHFTNTPSDQILHACKSTRFPD 60
           MASLSFLSFLSL FFF SLSLSSSS A  HHH H HLPSHFTN PSDQILHACKSTRFPD
Sbjct: 21  MASLSFLSFLSLFFFFSSLSLSSSS-ADHHHHPHPHLPSHFTNYPSDQILHACKSTRFPD 80

Query: 61  QCGSSLSHSSLKPPQNNPSPLQILQSAIALSSDGLTTAQSLVKQILDSSATNLNRTTAAK 120
           QCGSSLSHSSL P   NPSPLQILQSAIALSSDGLTTAQSLVKQILDSSATNLNRTTAAK
Sbjct: 81  QCGSSLSHSSLPP---NPSPLQILQSAIALSSDGLTTAQSLVKQILDSSATNLNRTTAAK 140

Query: 121 NCLEVLRNSDYRISLTNDALHRGRTKDARAWLSASLLYHYDCWSALKYANDTQLVDKTMA 180
           NCLEVLRNSDYRISLTNDALH GRTKDARAWLSASLLYHYDCWSALKYANDTQLVDKTMA
Sbjct: 141 NCLEVLRNSDYRISLTNDALHHGRTKDARAWLSASLLYHYDCWSALKYANDTQLVDKTMA 200

Query: 181 FLDSLIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGFWEGGGGGSGLGFSGGFPAKVT 240
           FLDSLIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGFWE GGGGSGLGFSGGFPAKVT
Sbjct: 201 FLDSLIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGFWE-GGGGSGLGFSGGFPAKVT 260

Query: 241 ADATVCKDGSGGCYGSIQKAVDAAPENAAARRFVIHIKEGVYEEIVRVPLEKKNVVFLGD 300
           ADATVCKDGSGGCYGSIQKAVDAAPENAAARRFVIHIKEGVYEEIVRVPLEKKNVVFLGD
Sbjct: 261 ADATVCKDGSGGCYGSIQKAVDAAPENAAARRFVIHIKEGVYEEIVRVPLEKKNVVFLGD 320

Query: 301 GMGKTVITGSLNAGQPGISTYNTATLGVLGDGFMASGLTIQNTAGPDAHQAVAFRSDSDL 360
           GMGKTVITGSLN GQPG+STYNTATLGV+GDGFMASGLTIQNTAGPDAHQAVAFRSDSDL
Sbjct: 321 GMGKTVITGSLNVGQPGLSTYNTATLGVVGDGFMASGLTIQNTAGPDAHQAVAFRSDSDL 380

Query: 361 SVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQDCEILIRPRQLKPEK 420
           SVIQDCEF+GNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQDCEIL+RPRQLKPEK
Sbjct: 381 SVIQDCEFLGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQDCEILVRPRQLKPEK 440

Query: 421 GENNAITAHGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKVHKNFLGRPWKEYSRTVF 480
           GENNA+TAHGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKP+VHKNFLGRPWKEYSRTVF
Sbjct: 441 GENNAVTAHGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPQVHKNFLGRPWKEYSRTVF 500

Query: 481 IHCNLEALVSPSGWMPWSGDFALKTLFYGEFENSGVGSKTSERVGWSSQIPAQHVFSYSV 540
           IHCNLEALVSPSGWMPWSGDFALKTLFYGEFENSG GSKTSERVGWSSQIPAQHVFSYSV
Sbjct: 501 IHCNLEALVSPSGWMPWSGDFALKTLFYGEFENSGAGSKTSERVGWSSQIPAQHVFSYSV 560

Query: 541 QNFIQGDEWIPSSESSNDQKMLNKKSKDVKLD 573
           +NFIQGDEWIPSSESS++QKMLNK+SKDVKLD
Sbjct: 561 ENFIQGDEWIPSSESSDEQKMLNKRSKDVKLD 587

BLAST of MELO3C023254 vs. TrEMBL
Match: B9RU20_RICCO (Pectinesterase OS=Ricinus communis GN=RCOM_1460660 PE=4 SV=1)

HSP 1 Score: 832.8 bits (2150), Expect = 2.5e-238
Identity = 404/554 (72.92%), Postives = 469/554 (84.66%), Query Frame = 1

Query: 5   SFLSFLSLSFFFFSLSLSSSSSAVRHHHSHSHLP--SHFTNTPSDQILHACKSTRFPDQC 64
           SFLSF  LS + F   LSS+S   RHH     LP  S  + +   +IL ACK+TRFPD C
Sbjct: 3   SFLSFSLLSLYLF---LSSTSVTSRHHTYRPKLPPPSSLSVSTPPEILQACKATRFPDTC 62

Query: 65  GSSLSHSSLKPPQNNPSPLQILQSAIALSSDGLTTAQSLVKQILDSSATNLNRTTAAKNC 124
            SSL+ S   P   NP+PLQI+QS++++S+  L TAQS+VK ILDSSA N+NRT AA+NC
Sbjct: 63  ASSLTSSDSVP--KNPTPLQIIQSSVSISATNLKTAQSMVKSILDSSAGNINRTNAARNC 122

Query: 125 LEVLRNSDYRISLTND-ALHRGRTKDARAWLSASLLYHYDCWSALKYANDTQLVDKTMAF 184
           LEVL NS+YRIS T D +L RGRTKDARAW+SA+LLY YDCWSALKYANDT   +KTM+F
Sbjct: 123 LEVLNNSEYRISSTADNSLPRGRTKDARAWMSAALLYQYDCWSALKYANDTSDTNKTMSF 182

Query: 185 LDSLIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGFWEGG--GGGSGLGFSGGFPAKV 244
           LDSL+ L+SN+LSM+++YDIYGNDT  WTPP+TERDGFWEG   G G GL F G FP+K+
Sbjct: 183 LDSLMLLTSNSLSMVMSYDIYGNDTKLWTPPKTERDGFWEGSEWGSGGGLVFRGVFPSKL 242

Query: 245 TADATVCKDGSGGCYGSIQKAVDAAPENAAARRFVIHIKEGVYEEIVRVPLEKKNVVFLG 304
           TAD TVCKDGS GCY ++Q+AV+ AP+N   RRFV+HIKEGVY EIVR+PLEKKNVVFLG
Sbjct: 243 TADVTVCKDGSNGCYKTVQEAVNTAPDNEMGRRFVVHIKEGVYNEIVRIPLEKKNVVFLG 302

Query: 305 DGMGKTVITGSLNAGQPGISTYNTATLGVLGDGFMASGLTIQNTAGPDAHQAVAFRSDSD 364
           DGMGKTVITGSL AGQPG+STYNTAT+GVLGDGFMASGLT QNTAG   HQAVAFRSDSD
Sbjct: 303 DGMGKTVITGSLTAGQPGVSTYNTATVGVLGDGFMASGLTFQNTAGAPTHQAVAFRSDSD 362

Query: 365 LSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQDCEILIRPRQLKPE 424
           LS I++CEFIG+QDTLYAHSLRQFYKSC+I+GNVDFIFGNSA+IFQDCEI++ PRQ KPE
Sbjct: 363 LSFIENCEFIGHQDTLYAHSLRQFYKSCRIEGNVDFIFGNSAAIFQDCEIVVNPRQEKPE 422

Query: 425 KGENNAITAHGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKVHKNFLGRPWKEYSRTV 484
           KGENNA+TAHGRTDPAQ+TGFVFQNCLINGT+EYM+L++SKP  HKN+LGRPWKEYSR V
Sbjct: 423 KGENNAVTAHGRTDPAQATGFVFQNCLINGTEEYMALFHSKPGAHKNYLGRPWKEYSRVV 482

Query: 485 FIHCNLEALVSPSGWMPWSGDFALKTLFYGEFENSGVGSKTSERVGWSSQIPAQHVFSYS 544
           FIHCN EA+++P GWMPW+GDFALKTL+YGEFENSG GS  S RV WSSQIPA+HV++YS
Sbjct: 483 FIHCNFEAIITPEGWMPWTGDFALKTLYYGEFENSGPGSNLSGRVKWSSQIPAEHVYTYS 542

Query: 545 VQNFIQGDEWIPSS 554
           VQNFIQGDEWIP+S
Sbjct: 543 VQNFIQGDEWIPTS 551

BLAST of MELO3C023254 vs. TrEMBL
Match: W9R8G4_9ROSA (Pectinesterase OS=Morus notabilis GN=L484_020726 PE=4 SV=1)

HSP 1 Score: 825.5 bits (2131), Expect = 4.1e-236
Identity = 415/578 (71.80%), Postives = 479/578 (82.87%), Query Frame = 1

Query: 1   MASLSFLSFLSLSFFFFSLSLSSSSSAVRHHHSHS------HLP-SHFTNTPSDQILHAC 60
           MASL FLS +SLS FF   SLS SS    HH  HS      H+P S   +T S  ++H C
Sbjct: 1   MASLLFLSIISLSLFF---SLSHSS----HHRGHSPPARVPHVPPSANPSTASSAVMHVC 60

Query: 61  KSTRFPDQCGSSLSHSSLKPPQNNPSPLQILQSAIALSSDGLTTAQSLVKQILDSSATNL 120
           K+TRFPD C  SL+  S      + SP Q++ +AI +SS+ L TAQS+V+ ILDSS+ N 
Sbjct: 61  KATRFPDVCEGSLARVS-----PDASPAQVIAAAIGVSSENLRTAQSMVRAILDSSSGNP 120

Query: 121 NRTTAAKNCLEVLRNSDYRISLTNDALHRGRTKDARAWLSASLLYHYDCWSALKYANDTQ 180
           NR+ AA NC+EVLRNS+YRIS  ++AL RGR KDARAW+SA+LLY YDCWSALKYANDTQ
Sbjct: 121 NRSIAAANCVEVLRNSEYRISAASEALPRGRNKDARAWMSAALLYQYDCWSALKYANDTQ 180

Query: 181 LVDKTMAFLDS-LIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGFWE-GGGGGSGLGF 240
           LV++TM+ LD+ LI LSSNALSM+ +YD +GN+TA+W PPRTERDGFWE G G GSGLG 
Sbjct: 181 LVNQTMSLLDTVLIRLSSNALSMMASYDNFGNETASWGPPRTERDGFWEKGSGSGSGLGS 240

Query: 241 SGGFPAKVTADATVCKDGSGGCYGSIQKAVDAAPENAAA-RRFVIHIKEGVYEEIVRVPL 300
            GGFP+K+TAD TVCK G G  Y  +Q AV+AAP      +RFVI +KEGVYEE VR+ +
Sbjct: 241 QGGFPSKLTADVTVCKGGGGCDYEKVQDAVNAAPNGVVGGKRFVIRVKEGVYEETVRIGI 300

Query: 301 EKKNVVFLGDGMGKTVITGSLNAGQPGISTYNTATLGVLGDGFMASGLTIQNTAGPDAHQ 360
           EKKNVV LGDGMGKTVITGSLN GQPGISTYNTAT+GVLGDGFMA+GLTIQNTAGPDAHQ
Sbjct: 301 EKKNVVLLGDGMGKTVITGSLNVGQPGISTYNTATVGVLGDGFMANGLTIQNTAGPDAHQ 360

Query: 361 AVAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQDCEIL 420
           AVAFRSDSDLS+I++CEFIGNQDTLYAHSLRQFYKSC+IQGNVDFIFGNSASIFQDCEIL
Sbjct: 361 AVAFRSDSDLSIIENCEFIGNQDTLYAHSLRQFYKSCRIQGNVDFIFGNSASIFQDCEIL 420

Query: 421 IRPRQLKPEKGENNAITAHGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKVHKNFLGR 480
           IRPRQLKPEKGENNA+TAHGRTDPAQSTGFVFQNCLINGT EYM LY SKP+VHKNFLGR
Sbjct: 421 IRPRQLKPEKGENNAVTAHGRTDPAQSTGFVFQNCLINGTDEYMKLYLSKPQVHKNFLGR 480

Query: 481 PWKEYSRTVFIHCNLEALVSPSGWMPWSGDFALKTLFYGEFENSGVGSKTSERVGWSSQI 540
           PWKEYSRTVFIHCNLEAL++P GWMPWS DFALKTLFYGEFENSG GS  S R+ WSSQI
Sbjct: 481 PWKEYSRTVFIHCNLEALITPQGWMPWSQDFALKTLFYGEFENSGAGSDVSRRMTWSSQI 540

Query: 541 PAQHVFSYSVQNFIQGDEWIPSSE----SSNDQKMLNK 565
           PA+HV++YSV++FIQGDEWIP++E    +S+ +KM+++
Sbjct: 541 PAEHVYAYSVESFIQGDEWIPTNEPPATTSHPRKMVHR 566

BLAST of MELO3C023254 vs. TrEMBL
Match: A0A061DIW1_THECC (Pectinesterase OS=Theobroma cacao GN=TCM_001430 PE=4 SV=1)

HSP 1 Score: 822.4 bits (2123), Expect = 3.4e-235
Identity = 402/557 (72.17%), Postives = 458/557 (82.23%), Query Frame = 1

Query: 1   MASLSFLSFLSLSFFFFSLSLSSSSSAVRHHHSHSHLP---SHFTNTPSDQILHACKSTR 60
           M++L  LS LSL+ FF      SSSS   HHH   HL    S  +  PS QI  AC+STR
Sbjct: 31  MSTLLLLSLLSLTLFF----TLSSSSRNHHHHQDPHLSPKNSPVSTPPSSQIHLACQSTR 90

Query: 61  FPDQCGSSLSHSSLKPPQNNPSPLQILQSAIALSSDGLTTAQSLVKQILDSSATNLNRTT 120
           FP  C +SL+ SS  PP  NP+ LQI+QSAIA+SS+ L T +S+VK ILDSS  NLNRT 
Sbjct: 91  FPQPCETSLTQSSSLPP--NPTSLQIIQSAIAVSSENLKTGKSMVKSILDSSKGNLNRTN 150

Query: 121 AAKNCLEVLRNSDYRISLTNDALHRGRTKDARAWLSASLLYHYDCWSALKYANDTQLVDK 180
           AA  CLE+L  SDYRI+  ND L RG+ KDARAW+SA+L Y YDCWSALKY NDT+LVD+
Sbjct: 151 AATTCLEILSYSDYRINSANDGLTRGKVKDARAWMSAALCYQYDCWSALKYVNDTKLVDQ 210

Query: 181 TMAFLDSLIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGFWEGGGGGSGLGFSGGFPA 240
           TMAFL SL   SSNAL+M+V+YD YGND A+W PP+TERDGF+E G GG+ LGF GGFP 
Sbjct: 211 TMAFLSSLTEHSSNALAMMVSYDNYGNDIASWAPPKTERDGFYESGSGGTDLGFKGGFPP 270

Query: 241 KVTADATVCKDGSGGCYGSIQKAVDAAPENA-AARRFVIHIKEGVYEEIVRVPLEKKNVV 300
           ++  D TVCKDGSGGCY ++Q AV+AAP NA   RRFVIHIKEGVYEE VRVP EKKNVV
Sbjct: 271 ELQPDVTVCKDGSGGCYKTVQDAVNAAPHNAETTRRFVIHIKEGVYEETVRVPFEKKNVV 330

Query: 301 FLGDGMGKTVITGSLNAGQPGISTYNTATLGVLGDGFMASGLTIQNTAGPDAHQAVAFRS 360
           FLGDGMGKT+ITGS N GQPG++TYN+AT+GVLGDGFMASGLTI+NTAGPDAHQAVAFRS
Sbjct: 331 FLGDGMGKTIITGSANVGQPGMTTYNSATVGVLGDGFMASGLTIKNTAGPDAHQAVAFRS 390

Query: 361 DSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQDCEILIRPRQL 420
           DSDLSVI++CEFIGNQDTLY HSLRQFYK C+IQGNVDFIFGNSAS+FQDC +L+ PRQL
Sbjct: 391 DSDLSVIENCEFIGNQDTLYVHSLRQFYKKCRIQGNVDFIFGNSASVFQDCIVLVAPRQL 450

Query: 421 KPEKGENNAITAHGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKVHKNFLGRPWKEYS 480
           KPEKGENNAITAHGRTDPAQSTG VFQNCL+NGT EYM  YYSKPKVH+NFLGRPWKEYS
Sbjct: 451 KPEKGENNAITAHGRTDPAQSTGLVFQNCLLNGTDEYMGYYYSKPKVHRNFLGRPWKEYS 510

Query: 481 RTVFIHCNLEALVSPSGWMPWSGDFALKTLFYGEFENSGVGSKTSERVGWSSQIPAQHVF 540
           RTVFI+C +EAL++P GW+PWSGD  LKTLFYGEF NSG GS  + RV WSSQIPAQ V+
Sbjct: 511 RTVFINCFMEALITPDGWLPWSGDVGLKTLFYGEFGNSGPGSNVASRVPWSSQIPAQRVY 570

Query: 541 SYSVQNFIQGDEWIPSS 554
           +YSVQNFIQGD+WIP+S
Sbjct: 571 TYSVQNFIQGDQWIPTS 581

BLAST of MELO3C023254 vs. TrEMBL
Match: A0A0B0NLF2_GOSAR (Pectinesterase OS=Gossypium arboreum GN=F383_18418 PE=4 SV=1)

HSP 1 Score: 815.1 bits (2104), Expect = 5.5e-233
Identity = 402/554 (72.56%), Postives = 461/554 (83.21%), Query Frame = 1

Query: 1   MASLSFLSFLSLSFFFFSLSLSSSSSAVRHHHSHSHLPSHFTNTPSDQILHACKSTRFPD 60
           M++   LS LSLS FF     SSSS    HH  H+   S    + S QI  ACKSTRFP 
Sbjct: 33  MSAFLLLSLLSLSHFFI---FSSSS----HHPPHN---SPVYASKSSQIRLACKSTRFPQ 92

Query: 61  QCGSSLSHSSLKPPQNNPSPLQILQSAIALSSDGLTTAQSLVKQILDSSATNLNRTTAAK 120
            C SSLS SSL PP  NP+ LQ +QSAI++SSD L T QS+VK ILDSS  NLNR+ AA 
Sbjct: 93  PCESSLSRSSLLPP--NPTSLQFIQSAISVSSDNLKTGQSMVKSILDSSKGNLNRSNAAA 152

Query: 121 NCLEVLRNSDYRISLTNDALHRGRTKDARAWLSASLLYHYDCWSALKYANDTQLVDKTMA 180
            CL +L NSDYR+  T+DAL RG+ KDARAW+SA+L Y YDCWSALKY NDT+LV +TMA
Sbjct: 153 ICLNILSNSDYRMRSTDDALARGKIKDARAWMSAALCYQYDCWSALKYVNDTKLVGETMA 212

Query: 181 FLDSLIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGFWEGGGGGSGLGFSGGFPAKVT 240
           FLDSL   SSNALSM+VAYD YG D AAW PP+TERDGF+E G GG+ LGF+GG P+ + 
Sbjct: 213 FLDSLTQHSSNALSMMVAYDNYGEDIAAWVPPKTERDGFYENGSGGTELGFNGGLPSNLK 272

Query: 241 ADATVCKDGSGGCYGSIQKAVDAAPENA-AARRFVIHIKEGVYEEIVRVPLEKKNVVFLG 300
            D TVCKDGSGGCY ++Q+AV+AAP NA + RRF+I+IKEGVYEE VRVPLEKKNVVFLG
Sbjct: 273 TDVTVCKDGSGGCYKTVQEAVNAAPANAESTRRFMIYIKEGVYEETVRVPLEKKNVVFLG 332

Query: 301 DGMGKTVITGSLNAGQPGISTYNTATLGVLGDGFMASGLTIQNTAGPDAHQAVAFRSDSD 360
           DGMGKT+ITG+LNAG PG++TY TAT+GVLGDGFMASGLTI+NTAGPDAHQAVAFRSDSD
Sbjct: 333 DGMGKTIITGALNAGMPGLTTYETATVGVLGDGFMASGLTIRNTAGPDAHQAVAFRSDSD 392

Query: 361 LSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQDCEILIRPRQLKPE 420
           LSVI++CEF+GNQDTLYAHSLRQFY+ C+IQGNVDFIFGNSAS+FQDCEIL+ PRQ+KPE
Sbjct: 393 LSVIENCEFLGNQDTLYAHSLRQFYRKCRIQGNVDFIFGNSASVFQDCEILVAPRQVKPE 452

Query: 421 KGENNAITAHGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKVHKNFLGRPWKEYSRTV 480
           KGENNA+TAHGRTDPAQSTG VFQNCLINGT EYM  YY KPKVHKNFLGRPWKEYSRTV
Sbjct: 453 KGENNAVTAHGRTDPAQSTGLVFQNCLINGTDEYMRYYYGKPKVHKNFLGRPWKEYSRTV 512

Query: 481 FIHCNLEALVSPSGWMPWSGDFALKTLFYGEFENSGVGSKTSERVGWSSQIPAQHVFSYS 540
           FI+C +EAL++P+GWMPW GDFALKTLFYGEF NSG+GS    RV WS+QIP+QHV ++S
Sbjct: 513 FINCVMEALINPNGWMPWKGDFALKTLFYGEFGNSGLGSIPLNRVPWSTQIPSQHVHTFS 572

Query: 541 VQNFIQGDEWIPSS 554
           VQNFIQGD+WIP+S
Sbjct: 573 VQNFIQGDQWIPTS 574

BLAST of MELO3C023254 vs. TAIR10
Match: AT5G09760.1 (AT5G09760.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 726.5 bits (1874), Expect = 1.3e-209
Identity = 362/551 (65.70%), Postives = 426/551 (77.31%), Query Frame = 1

Query: 11  SLSFFFFSLSL-SSSSSAVRHHHSHSHLPSHFTNTPSD---QILHACKSTRFPDQCGSSL 70
           S+    FSL L SS SS+ RHHH H+   +   N  S    QI  AC +TR+PDQC SSL
Sbjct: 3   SILILLFSLFLFSSPSSSSRHHHHHNSGDTSPVNPSSSLAAQIRLACNATRYPDQCVSSL 62

Query: 71  SHSSLKPPQNNPSPLQILQSAIALSSDGLTTAQSLVKQILDSSATNLNRTTAAKNCLEVL 130
           S     PP  +P P+QI+ SAI+ S   L TAQS +K I+DSS  NLNRT AA  CL++L
Sbjct: 63  SEQGRVPP--DPKPIQIIHSAISFSFQNLKTAQSKIKSIVDSSVGNLNRTNAANTCLQLL 122

Query: 131 RNSDYRISLTNDALHRGRTKDARAWLSASLLYHYDCWSALKYANDTQLVDKTMAFLDSLI 190
             S++R   T+ AL RG+ KDARAW+SA+L+Y YD WSALKY NDT  V +TM+FLD LI
Sbjct: 123 TYSEHRTQSTDQALTRGKIKDARAWMSAALVYQYDSWSALKYVNDTSQVGETMSFLDGLI 182

Query: 191 GLSSNALSMLVAYDIYGNDTAAWTPPRTERDGFWEGGGGGSGL----GFSGGFPAKVTAD 250
            ++SNALSM+V+YD +G++ A+WT P TERDGFWE  G G GL    G + GFP+ +  D
Sbjct: 183 HVTSNALSMMVSYDNFGDNVASWTYPATERDGFWEKTGPGLGLDPSTGLNLGFPSGLKED 242

Query: 251 ATVCKDGSGGCYGSIQKAVDAAPENAAARRFVIHIKEGVYEEIVRVPLEKKNVVFLGDGM 310
            TVCKDG  G Y ++Q AV+AAPE+   R+FVI I EGVYEE V VP EKKNVVF+GDGM
Sbjct: 243 VTVCKDGKCG-YKTVQDAVNAAPEDNGMRKFVIKISEGVYEENVIVPFEKKNVVFIGDGM 302

Query: 311 GKTVITGSLNAGQPGISTYNTATLGVLGDGFMASGLTIQNTAGPDAHQAVAFRSDSDLSV 370
           GKTVITGSLNAG PGI+TYNTAT+GV+GDGFMA  LT QNTAGPDAHQAVAFRSDSD S+
Sbjct: 303 GKTVITGSLNAGMPGITTYNTATVGVVGDGFMARDLTFQNTAGPDAHQAVAFRSDSDFSL 362

Query: 371 IQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQDCEILIRPRQLKPEKGE 430
           I++CEF+GNQDTLYAH LRQFYK+C+IQGNVDFIFGNSA++FQDCEILI PRQ+ PEKGE
Sbjct: 363 IENCEFLGNQDTLYAHGLRQFYKNCRIQGNVDFIFGNSAAVFQDCEILIAPRQINPEKGE 422

Query: 431 NNAITAHGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKVHKNFLGRPWKEYSRTVFIH 490
            NA+TA GR DP+QSTGFVF NCLINGT+EYM L+ + PKVHKNFLGRPWK+YSRTVFI 
Sbjct: 423 KNAVTAQGRIDPSQSTGFVFLNCLINGTEEYMKLFKANPKVHKNFLGRPWKDYSRTVFIG 482

Query: 491 CNLEALVSPSGWMPWSGDFALKTLFYGEFENSGVGSKTSERVGWSSQIPAQHVFSYSVQN 550
           CNLEAL++P GW+PWSGDFALKTL+YGE +N+G GS  S+RV WSSQIP +HV  YSV N
Sbjct: 483 CNLEALITPDGWLPWSGDFALKTLYYGESKNTGPGSDRSQRVSWSSQIPDEHVHVYSVAN 542

Query: 551 FIQGDEWIPSS 554
           FIQ DEW   S
Sbjct: 543 FIQADEWASMS 550

BLAST of MELO3C023254 vs. TAIR10
Match: AT5G64640.1 (AT5G64640.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 656.0 bits (1691), Expect = 2.2e-188
Identity = 327/551 (59.35%), Postives = 405/551 (73.50%), Query Frame = 1

Query: 19  LSLSSSSSAVRHHHSHSHLPSHFTNTPSD--------------QILHACKSTRFPDQCGS 78
           L  ++ S + R+HH+    PSH T T  D              QI  AC +TRFPD C +
Sbjct: 53  LIYATVSKSSRNHHN----PSHQTPTSDDHPPPETPPSPPPIAQIRLACNATRFPDHCVA 112

Query: 79  SLSHSSLKPPQNNPSPLQILQSAIALSSDGLTTAQSLVKQILDSSATNLNRTTAAKNCLE 138
           SLS     PP  +P P+QI+ SAI++S + L + QS ++ ILDSSA N NRT  A  CLE
Sbjct: 113 SLSKPGQVPP--DPKPVQIIHSAISVSYENLKSGQSKIQSILDSSAGNRNRTNIATICLE 172

Query: 139 VLRNSDYRISLTNDALHRGRTKDARAWLSASLLYHYDCWSALKYANDTQLVDKTMAFLDS 198
           +L  S +R   T+ A+  G  KDARAW+SA+L Y +DCWS LK  NDT+ V  T+ F + 
Sbjct: 173 ILSYSQHRTESTDIAVTSGDIKDARAWMSAALAYQFDCWSGLKTVNDTKQVVDTITFFEG 232

Query: 199 LIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGFWEGGG----GGSGLGFSGGFPAKVT 258
           L+ L+ NALSM++++D +G+D  +W  P TERDGFWE  G     G+G   S GFP+ +T
Sbjct: 233 LVNLTGNALSMMLSFDSFGDDVVSWIRPATERDGFWEKAGPSLGSGTGTEASLGFPSGLT 292

Query: 259 ADATVCKDGSGGC-YGSIQKAVDAAPENAAARRFVIHIKEGVYEEIVRVPLEKKNVVFLG 318
            D TVCK+G   C Y ++Q+AVD+AP+     +FVI I+EGVYEE VRVP EKKNVVF+G
Sbjct: 293 EDVTVCKNGGKDCKYKTVQEAVDSAPDTNRTVKFVIRIREGVYEETVRVPFEKKNVVFIG 352

Query: 319 DGMGKTVITGSLNAGQPGISTYNTATLGVLGDGFMASGLTIQNTAGPDAHQAVAFRSDSD 378
           DGMGKTVITGSLN GQPG++T+ +AT+GVLGDGFMA  LTI+NTAG DAHQAVAFRSDSD
Sbjct: 353 DGMGKTVITGSLNVGQPGMTTFESATVGVLGDGFMARDLTIENTAGADAHQAVAFRSDSD 412

Query: 379 LSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQDCEILIRPRQLKPE 438
            SV+++CEF+GNQDTLYAHSLRQFYK C+IQGNVDFIFGNSA++FQDC+ILI  +  K E
Sbjct: 413 FSVLENCEFLGNQDTLYAHSLRQFYKQCRIQGNVDFIFGNSAAVFQDCDILIASKHSKLE 472

Query: 439 K-GENNAITAHGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKVHKNFLGRPWKEYSRT 498
           + G NNAITAHGR D +QSTGFVF NC INGT+EYM  + + P+ HKNFLGRPWKE+SRT
Sbjct: 473 QGGANNAITAHGRIDASQSTGFVFLNCSINGTEEYMKEFQANPEGHKNFLGRPWKEFSRT 532

Query: 499 VFIHCNLEALVSPSGWMPWSGDFALKTLFYGEFENSGVGSKTSERVGWSSQIPAQHVFSY 550
           VF++CNLE+L+SP GWMPW+GDFALKTL+YGE++N+G GS  S RV WSS+IP +HV  Y
Sbjct: 533 VFVNCNLESLISPDGWMPWNGDFALKTLYYGEYKNTGPGSVRSSRVPWSSEIPEKHVDVY 592

BLAST of MELO3C023254 vs. TAIR10
Match: AT2G45220.1 (AT2G45220.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 308.5 bits (789), Expect = 8.4e-84
Identity = 191/505 (37.82%), Postives = 271/505 (53.66%), Query Frame = 1

Query: 53  CKSTRFPDQCGSSLSHSSLKPPQNNPSPLQILQSAIALSSDGLTTAQSLVKQILDSSATN 112
           C  T  P  C   L+H+S   P  + S    +   + L    L    +     L     +
Sbjct: 34  CSQTPNPKPCEYFLTHNSNNEPIKSESEFLKISMKLVLDRAILAKTHAFT---LGPKCRD 93

Query: 113 LNRTTAAKNCLEVLRNSDYRISLTNDALHRGRTKDARAWLSASLLYHYDCWSALKYANDT 172
                A ++C+++   +  +I+ T D   +    DA+ WLS +L     C +       T
Sbjct: 94  TREKAAWEDCIKLYDLTVSKINETMDPNVKCSKLDAQTWLSTALTNLDTCRAGFLELGVT 153

Query: 173 QLVDKTMAFLDSLIGLSSNALSMLVAYDIYGNDTAA-WTPPRTERDGFWEGGGGGSGLGF 232
            +V          + L SN +S L+   +  N     +TPP  E+DGF      G     
Sbjct: 154 DIV----------LPLMSNNVSNLLCNTLAINKVPFNYTPP--EKDGFPSWVKPGDRKLL 213

Query: 233 SGGFPAKVTADATVCKDGSGGCYGSIQKAVDAAPENAAARRFVIHIKEGVYEEIVRVPLE 292
               P     +A V KDGSG  + +I++A+DAA   + + RFVI++K+GVY E   + + 
Sbjct: 214 QSSTPKD---NAVVAKDGSGN-FKTIKEAIDAA---SGSGRFVIYVKQGVYSE--NLEIR 273

Query: 293 KKNVVFLGDGMGKTVITGSLNAGQPGISTYNTATLGVLGDGFMASGLTIQNTAGPDAHQA 352
           KKNV+  GDG+GKT+ITGS + G  G +T+N+AT+  +GDGF+A G+T +NTAG    QA
Sbjct: 274 KKNVMLRGDGIGKTIITGSKSVGG-GTTTFNSATVAAVGDGFIARGITFRNTAGASNEQA 333

Query: 353 VAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQDCEILI 412
           VA RS SDLSV   C F   QDTLY HS RQFY+ C + G VDFIFGN+A++ Q+C I  
Sbjct: 334 VALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAVLQNCNIFA 393

Query: 413 RPRQLKPEKGENNAITAHGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKVHKNFLGRP 472
           R    +P + + N ITA GR+DP Q+TG +  N  +    +   +  S     K +LGRP
Sbjct: 394 R----RP-RSKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGST----KTYLGRP 453

Query: 473 WKEYSRTVFIHCNLEALVSPSGWMPWSGDFALKTLFYGEFENSGVGSKTSERVGWSS--- 532
           W++YSRTVF+  +L++L+ P GW+ W G+FALKTLFY EF+N+G G+ TS RV W     
Sbjct: 454 WRQYSRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGFRV 504

Query: 533 QIPAQHVFSYSVQNFIQGDEWIPSS 554
              A     ++V  F+ G  WIPSS
Sbjct: 514 LGSASEASKFTVGTFLAGGSWIPSS 504

BLAST of MELO3C023254 vs. TAIR10
Match: AT3G06830.1 (AT3G06830.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 307.4 bits (786), Expect = 1.9e-83
Identity = 192/559 (34.35%), Postives = 288/559 (51.52%), Query Frame = 1

Query: 17  FSLSLSSSSSAVRHHHSHSHLPSHFTNTPSDQILHACKSTRFPDQCGSSLSHSSLKPPQN 76
           F + +  S + V   HS     +H   T +  +   C  T F D C +SL  +S  P  +
Sbjct: 20  FLVIMVVSVAVVTSKHSPKDDENHIRKT-TKAVQAVCAPTDFKDTCVNSLMGAS--PDSD 79

Query: 77  NPSPLQILQSAIALSS--DGLTTAQSLVKQILDSSATNLNRTTAAKNCL-----EVLRNS 136
           +P  L  L   + + S  + L  A   +K   D +          +  +     ++ +  
Sbjct: 80  DPVDLIKLGFKVTIKSINESLEKASGDIKAKADKNPEAKGAFELCEKLMIDAIDDLKKCM 139

Query: 137 DYRISLTNDALHRGRTKDARAWLSASLLYHYDCWSALKYANDTQLVDKTMAFLDSLIGLS 196
           D+  S+    +     +D R WLS S+ +   C  +        + D    F  S   LS
Sbjct: 140 DHGFSVDQIEVF---VEDLRVWLSGSIAFQQTCMDSFGEIKSNLMQDMLKIFKTSR-ELS 199

Query: 197 SNALSMLVAYD--IYGNDTAAWTPPRTE--------RDGF--WEGGGGGSGLGFSGGFPA 256
           SN+L+M+      I  ++    T    +         D    W G      +   GG P 
Sbjct: 200 SNSLAMVTRISTLIPNSNLTGLTGALAKYARKLLSTEDSIPTWVGPEARRLMAAQGGGPG 259

Query: 257 KVTADATVCKDGSGGCYGSIQKAVDAAPENAAARRFVIHIKEGVYEEIVRVPLEKKNVVF 316
            V A+A V +DG+G  + +I  A++A P+      F+IHIKEG+Y+E V V  +  +V F
Sbjct: 260 PVKANAVVAQDGTGQ-FKTITDALNAVPKGNKVP-FIIHIKEGIYKEKVTVTKKMPHVTF 319

Query: 317 LGDGMGKTVITGSLNAGQPGISTYNTATLGVLGDGFMASGLTIQNTAGPDAHQAVAFRSD 376
           +GDG  KT+ITGSLN G   + T+ TAT+ + GD F A  + I+NTAGP+  QAVA R  
Sbjct: 320 IGDGPNKTLITGSLNFGIGKVKTFLTATITIEGDHFTAKNIGIENTAGPEGGQAVALRVS 379

Query: 377 SDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQDCEILIRPRQLK 436
           +D +V   C+  G+QDTLY HS RQFY+ C + G VDFIFG++  I Q+C+I++R    K
Sbjct: 380 ADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTVSGTVDFIFGDAKCILQNCKIVVR----K 439

Query: 437 PEKGENNAITAHGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKVHKNFLGRPWKEYSR 496
           P KG+   +TA GR++  +STG V   C I G   Y+ +      V+K +LGRPWKE+SR
Sbjct: 440 PNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDPAYIPM----KSVNKAYLGRPWKEFSR 499

Query: 497 TVFIHCNLEALVSPSGWMPWSGDFALKTLFYGEFENSGVGSKTSERVGWS--SQIPAQHV 555
           T+ +   ++ ++ P+GW+PWSGDFALKTL+Y E  N+G GS  ++RV W    ++  Q  
Sbjct: 500 TIIMKTTIDDVIDPAGWLPWSGDFALKTLYYAEHMNTGPGSNQAQRVKWPGIKKLTPQDA 559

BLAST of MELO3C023254 vs. TAIR10
Match: AT1G23200.1 (AT1G23200.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 306.6 bits (784), Expect = 3.2e-83
Identity = 202/554 (36.46%), Postives = 288/554 (51.99%), Query Frame = 1

Query: 33  SHSHLPSHFTNTPSDQILHACKSTRFPDQCGSSLSHSSLKPPQNNPSPLQILQSAIALSS 92
           S SHL +HF        + +CK T +P  C   +S+S LK   +           ++ + 
Sbjct: 26  SISHLNAHF--------ITSCKQTPYPSVCDHHMSNSPLKTLDDQTDGFTFHDLVVSSTM 85

Query: 93  DGLTTAQSLVKQILDSSATNLNRTTAAKNCLEVLRNSDYRISLTNDALHR-GRTKDARAW 152
           D       LV  +    + + + T+A  +CLE+  ++  +++ +  +  +     D +  
Sbjct: 86  DQAVQLHRLVSSLKQHHSLHKHATSALFDCLELYEDTIDQLNHSRRSYGQYSSPHDRQTS 145

Query: 153 LSASLLYHYDCWSALKYANDTQLVDK--TMAFLDSLIGLSSNALSMLVAYDIYGNDTAAW 212
           LSA++     C +  +    T    K   + F  +L    SN+L++  A      +  A 
Sbjct: 146 LSAAIANQDTCRNGFRDFKLTSSYSKYFPVQFHRNLTKSISNSLAVTKA--AAEAEAVAE 205

Query: 213 TPPRTERDGFWE---GGGGGSGLG--------FSGGFP---------AKVTA--DATVCK 272
             P T    F +     GGGS           F   FP         +K TA  D  V K
Sbjct: 206 KYPSTGFTKFSKQRSSAGGGSHRRLLLFSDEKFPSWFPLSDRKLLEDSKTTAKADLVVAK 265

Query: 273 DGSGGCYGSIQKAVDAAPENAAA-RRFVIHIKEGVYEEIVRVPLEKKNVVFLGDGMGKTV 332
           DGSG  Y SIQ+AV+AA +     +R VI++K GVY E V +    KNV+ +GDG+  T+
Sbjct: 266 DGSGH-YTSIQQAVNAAAKLPRRNQRLVIYVKAGVYRENVVIKKSIKNVMVIGDGIDSTI 325

Query: 333 ITGSLNAGQPGISTYNTATLGVLGDGFMASGLTIQNTAGPDAHQAVAFRSDSDLSVIQDC 392
           +TG+ N  Q G +T+ +AT  V G+GF+A G+T +NTAGP+ HQAVA RS SD SV   C
Sbjct: 326 VTGNRNV-QDGTTTFRSATFAVSGNGFIAQGITFENTAGPEKHQAVALRSSSDFSVFYAC 385

Query: 393 EFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQDCEILIRPRQLKPEKGENNAI 452
            F G QDTLY HS RQF ++C I G VDFIFG++ +I Q+C I  R    KP  G+ N I
Sbjct: 386 SFKGYQDTLYLHSSRQFLRNCNIYGTVDFIFGDATAILQNCNIYAR----KPMSGQKNTI 445

Query: 453 TAHGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKVHKNFLGRPWKEYSRTVFIHCNLE 512
           TA  R +P ++TGFV Q+  +    E              +LGRPW+ +SRTVF+ CNL 
Sbjct: 446 TAQSRKEPDETTGFVIQSSTVATASE-------------TYLGRPWRSHSRTVFMKCNLG 505

Query: 513 ALVSPSGWMPWSGDFALKTLFYGEFENSGVGSKTSERVGWSSQIPAQHVF-------SYS 554
           ALVSP+GW+PWSG FAL TL+YGE+ N+G G+  S RV W    P  HV         ++
Sbjct: 506 ALVSPAGWLPWSGSFALSTLYYGEYGNTGAGASVSGRVKW----PGYHVIKTVTEAEKFT 546

BLAST of MELO3C023254 vs. NCBI nr
Match: gi|659121958|ref|XP_008460902.1| (PREDICTED: LOW QUALITY PROTEIN: probable pectinesterase/pectinesterase inhibitor 51 [Cucumis melo])

HSP 1 Score: 1148.7 bits (2970), Expect = 0.0e+00
Identity = 568/572 (99.30%), Postives = 568/572 (99.30%), Query Frame = 1

Query: 1   MASLSFLSFLSLSFFFFSLSLSSSSSAVRHHHSHSHLPSHFTNTPSDQILHACKSTRFPD 60
           MASLSFLSFLSLSFFFFSLSLSSSSSAVRHHHSHSHLPSHFTNTPSDQILHACKSTRFPD
Sbjct: 21  MASLSFLSFLSLSFFFFSLSLSSSSSAVRHHHSHSHLPSHFTNTPSDQILHACKSTRFPD 80

Query: 61  QCGSSLSHSSLKPPQNNPSPLQILQSAIALSSDGLTTAQSLVKQILDSSATNLNRTTAAK 120
           QCGSSLSHSS  PP  NPSPLQILQSAIALSSDGLTTAQSLVKQILDSSATNLNRTTAAK
Sbjct: 81  QCGSSLSHSSXSPP--NPSPLQILQSAIALSSDGLTTAQSLVKQILDSSATNLNRTTAAK 140

Query: 121 NCLEVLRNSDYRISLTNDALHRGRTKDARAWLSASLLYHYDCWSALKYANDTQLVDKTMA 180
           NCLEVLRNSDYRISLTNDALHRGRTKDARAWLSASLLYHYDCWSALKYANDTQLVDKTMA
Sbjct: 141 NCLEVLRNSDYRISLTNDALHRGRTKDARAWLSASLLYHYDCWSALKYANDTQLVDKTMA 200

Query: 181 FLDSLIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGFWEGGGGGSGLGFSGGFPAKVT 240
           FLDSLIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGFWEGGGGGSGLGFSGGFPAKVT
Sbjct: 201 FLDSLIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGFWEGGGGGSGLGFSGGFPAKVT 260

Query: 241 ADATVCKDGSGGCYGSIQKAVDAAPENAAARRFVIHIKEGVYEEIVRVPLEKKNVVFLGD 300
           ADATVCKDGSGGCYGSIQKAVDAAPENAAARRFVIHIKEGVYEEIVRVPLEKKNVVFLGD
Sbjct: 261 ADATVCKDGSGGCYGSIQKAVDAAPENAAARRFVIHIKEGVYEEIVRVPLEKKNVVFLGD 320

Query: 301 GMGKTVITGSLNAGQPGISTYNTATLGVLGDGFMASGLTIQNTAGPDAHQAVAFRSDSDL 360
           GMGKTVITGSLNAGQPGISTYNTATLGVLGDGFMASGLTIQNTAGPDAHQAVAFRSDSDL
Sbjct: 321 GMGKTVITGSLNAGQPGISTYNTATLGVLGDGFMASGLTIQNTAGPDAHQAVAFRSDSDL 380

Query: 361 SVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQDCEILIRPRQLKPEK 420
           SVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQDCEILIRPRQLKPEK
Sbjct: 381 SVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQDCEILIRPRQLKPEK 440

Query: 421 GENNAITAHGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKVHKNFLGRPWKEYSRTVF 480
           GENNAITAHGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKVHKNFLGRPWKEYSRTVF
Sbjct: 441 GENNAITAHGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKVHKNFLGRPWKEYSRTVF 500

Query: 481 IHCNLEALVSPSGWMPWSGDFALKTLFYGEFENSGVGSKTSERVGWSSQIPAQHVFSYSV 540
           IHCNLEALVSPSGWMPWSGDFALKTLFYGEFENSGVGSKTSERVGWSSQIPAQHVFSYSV
Sbjct: 501 IHCNLEALVSPSGWMPWSGDFALKTLFYGEFENSGVGSKTSERVGWSSQIPAQHVFSYSV 560

Query: 541 QNFIQGDEWIPSSESSNDQKMLNKKSKDVKLD 573
           QNFIQGDEWIPSSESSNDQKMLNKKSKDVKLD
Sbjct: 561 QNFIQGDEWIPSSESSNDQKMLNKKSKDVKLD 590

BLAST of MELO3C023254 vs. NCBI nr
Match: gi|778670202|ref|XP_004147375.2| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 51 [Cucumis sativus])

HSP 1 Score: 1101.7 bits (2848), Expect = 0.0e+00
Identity = 546/572 (95.45%), Postives = 556/572 (97.20%), Query Frame = 1

Query: 1   MASLSFLSFLSLSFFFFSLSLSSSSSAVRHHHSHSHLPSHFTNTPSDQILHACKSTRFPD 60
           MASLSFLSFLSL FFF SLSLSSSS A  HHH H HLPSHFTN PSDQILHACKSTRFPD
Sbjct: 21  MASLSFLSFLSLFFFFSSLSLSSSS-ADHHHHPHPHLPSHFTNYPSDQILHACKSTRFPD 80

Query: 61  QCGSSLSHSSLKPPQNNPSPLQILQSAIALSSDGLTTAQSLVKQILDSSATNLNRTTAAK 120
           QCGSSLSHSSL P   NPSPLQILQSAIALSSDGLTTAQSLVKQILDSSATNLNRTTAAK
Sbjct: 81  QCGSSLSHSSLPP---NPSPLQILQSAIALSSDGLTTAQSLVKQILDSSATNLNRTTAAK 140

Query: 121 NCLEVLRNSDYRISLTNDALHRGRTKDARAWLSASLLYHYDCWSALKYANDTQLVDKTMA 180
           NCLEVLRNSDYRISLTNDALH GRTKDARAWLSASLLYHYDCWSALKYANDTQLVDKTMA
Sbjct: 141 NCLEVLRNSDYRISLTNDALHHGRTKDARAWLSASLLYHYDCWSALKYANDTQLVDKTMA 200

Query: 181 FLDSLIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGFWEGGGGGSGLGFSGGFPAKVT 240
           FLDSLIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGFWE GGGGSGLGFSGGFPAKVT
Sbjct: 201 FLDSLIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGFWE-GGGGSGLGFSGGFPAKVT 260

Query: 241 ADATVCKDGSGGCYGSIQKAVDAAPENAAARRFVIHIKEGVYEEIVRVPLEKKNVVFLGD 300
           ADATVCKDGSGGCYGSIQKAVDAAPENAAARRFVIHIKEGVYEEIVRVPLEKKNVVFLGD
Sbjct: 261 ADATVCKDGSGGCYGSIQKAVDAAPENAAARRFVIHIKEGVYEEIVRVPLEKKNVVFLGD 320

Query: 301 GMGKTVITGSLNAGQPGISTYNTATLGVLGDGFMASGLTIQNTAGPDAHQAVAFRSDSDL 360
           GMGKTVITGSLN GQPG+STYNTATLGV+GDGFMASGLTIQNTAGPDAHQAVAFRSDSDL
Sbjct: 321 GMGKTVITGSLNVGQPGLSTYNTATLGVVGDGFMASGLTIQNTAGPDAHQAVAFRSDSDL 380

Query: 361 SVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQDCEILIRPRQLKPEK 420
           SVIQDCEF+GNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQDCEIL+RPRQLKPEK
Sbjct: 381 SVIQDCEFLGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQDCEILVRPRQLKPEK 440

Query: 421 GENNAITAHGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKVHKNFLGRPWKEYSRTVF 480
           GENNA+TAHGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKP+VHKNFLGRPWKEYSRTVF
Sbjct: 441 GENNAVTAHGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPQVHKNFLGRPWKEYSRTVF 500

Query: 481 IHCNLEALVSPSGWMPWSGDFALKTLFYGEFENSGVGSKTSERVGWSSQIPAQHVFSYSV 540
           IHCNLEALVSPSGWMPWSGDFALKTLFYGEFENSG GSKTSERVGWSSQIPAQHVFSYSV
Sbjct: 501 IHCNLEALVSPSGWMPWSGDFALKTLFYGEFENSGAGSKTSERVGWSSQIPAQHVFSYSV 560

Query: 541 QNFIQGDEWIPSSESSNDQKMLNKKSKDVKLD 573
           +NFIQGDEWIPSSESS++QKMLNK+SKDVKLD
Sbjct: 561 ENFIQGDEWIPSSESSDEQKMLNKRSKDVKLD 587

BLAST of MELO3C023254 vs. NCBI nr
Match: gi|1009146861|ref|XP_015891098.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 51 [Ziziphus jujuba])

HSP 1 Score: 839.7 bits (2168), Expect = 3.0e-240
Identity = 415/561 (73.98%), Postives = 477/561 (85.03%), Query Frame = 1

Query: 1   MASLSFLSFLSLSFFFFSLSLSSSSSAVRHHHSHSHLPSHFTNTPSDQIL-----HACKS 60
           MASL  LS +SL  FF   SLSS+S+    HHSH H P      P+   L      ACK+
Sbjct: 26  MASLLILSLISLLLFF---SLSSASN----HHSH-HNPKIIPTVPTSSSLPAAIQQACKA 85

Query: 61  TRFPDQCGSSLSHSSLKPPQNNPSPLQILQSAIALSSDGLTTAQSLVKQILDSSATNLNR 120
           TRFP+QC +SLS S   PP  NPS ++I+QSAI++SS+ L TA S+VK ILDSS+ + NR
Sbjct: 86  TRFPEQCQTSLSQSPHLPP--NPSSVEIVQSAISVSSENLKTALSMVKSILDSSSGSQNR 145

Query: 121 TTAAKNCLEVLRNSDYRISLTNDALHRGRTKDARAWLSASLLYHYDCWSALKYANDTQLV 180
           TTAA NCLEVL NS+YR SL   A   G+ KDARAW+SA+LL+ YDCWSALKYANDT++V
Sbjct: 146 TTAANNCLEVLHNSEYRTSLAGKAFPAGKMKDARAWMSAALLFQYDCWSALKYANDTKMV 205

Query: 181 DKTMAFLDSLIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGFW-EGGGGGSGLGFSGG 240
           ++TM+FLDSLIGLSSNALSM+ +YD +G  T +W PP+TERDGFW  G GGGS LG  GG
Sbjct: 206 NETMSFLDSLIGLSSNALSMMASYDNFGAKTESWVPPKTERDGFWGNGSGGGSELGSGGG 265

Query: 241 FPAKVTADATVCKDGSGGCYGSIQKAVDAAPENAAA-RRFVIHIKEGVYEEIVRVPLEKK 300
           FP+K+TADATVCKDG GGCY ++Q AV+AAP+NA   ++FVIHIKEGVYEE VRVPLEKK
Sbjct: 266 FPSKLTADATVCKDGRGGCYKTVQDAVNAAPDNAVVGKKFVIHIKEGVYEETVRVPLEKK 325

Query: 301 NVVFLGDGMGKTVITGSLNAGQPGISTYNTATLGVLGDGFMASGLTIQNTAGPDAHQAVA 360
           NVVFLGDGMGKTVITGSLN GQPGISTYNTAT+GVLGDGFMASGLTI+NTAGPDAHQAVA
Sbjct: 326 NVVFLGDGMGKTVITGSLNVGQPGISTYNTATVGVLGDGFMASGLTIKNTAGPDAHQAVA 385

Query: 361 FRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQDCEILIRP 420
           FRSDSDLSVIQ+CEF+GNQDTLYAH+LRQFYKSC+IQGNVDFIFGNSAS+FQDC+I + P
Sbjct: 386 FRSDSDLSVIQNCEFLGNQDTLYAHALRQFYKSCRIQGNVDFIFGNSASVFQDCQIFVAP 445

Query: 421 RQLKPEKGENNAITAHGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKVHKNFLGRPWK 480
           RQLKPEKGENNA+TA+GRTDPAQSTGFVFQNCLINGT+EYM LYYSKPKVHKNFLGRPWK
Sbjct: 446 RQLKPEKGENNAVTANGRTDPAQSTGFVFQNCLINGTEEYMKLYYSKPKVHKNFLGRPWK 505

Query: 481 EYSRTVFIHCNLEALVSPSGWMPWSGDFALKTLFYGEFENSGVGSKTSERVGWSSQIPAQ 540
           EYSRTVFI+CN+E+L++P GWMPWSGDFALKTLFYGEF NSG GS  S+RV WSS+IPA+
Sbjct: 506 EYSRTVFINCNIESLLAPQGWMPWSGDFALKTLFYGEFGNSGPGSNLSQRVAWSSRIPAE 565

Query: 541 HVFSYSVQNFIQGDEWIPSSE 555
           HV +YSVQNFIQGDEWIP+S+
Sbjct: 566 HVNAYSVQNFIQGDEWIPTSQ 576

BLAST of MELO3C023254 vs. NCBI nr
Match: gi|1000970516|ref|XP_002517239.2| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 51 isoform X1 [Ricinus communis])

HSP 1 Score: 835.1 bits (2156), Expect = 7.4e-239
Identity = 404/556 (72.66%), Postives = 469/556 (84.35%), Query Frame = 1

Query: 3   SLSFLSFLSLSFFFFSLSLSSSSSAVRHHHSHSHLP--SHFTNTPSDQILHACKSTRFPD 62
           S+S  SFLS S     L LSS+S   RHH     LP  S  + +   +IL ACK+TRFPD
Sbjct: 21  SISMASFLSFSLLSLYLFLSSTSVTSRHHTYRPKLPPPSSLSVSTPPEILQACKATRFPD 80

Query: 63  QCGSSLSHSSLKPPQNNPSPLQILQSAIALSSDGLTTAQSLVKQILDSSATNLNRTTAAK 122
            C SSL+ S   P   NP+PLQI+QS++++S+  L TAQS+VK ILDSSA N+NRT AA+
Sbjct: 81  TCASSLTSSDSVP--KNPTPLQIIQSSVSISATNLKTAQSMVKSILDSSAGNINRTNAAR 140

Query: 123 NCLEVLRNSDYRISLTND-ALHRGRTKDARAWLSASLLYHYDCWSALKYANDTQLVDKTM 182
           NCLEVL NS+YRIS T D +L RGRTKDARAW+SA+LLY YDCWSALKYANDT   +KTM
Sbjct: 141 NCLEVLNNSEYRISSTADNSLPRGRTKDARAWMSAALLYQYDCWSALKYANDTSDTNKTM 200

Query: 183 AFLDSLIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGFWEGG--GGGSGLGFSGGFPA 242
           +FLDSL+ L+SN+LSM+++YDIYGNDT  WTPP+TERDGFWEG   G G GL F G FP+
Sbjct: 201 SFLDSLMLLTSNSLSMVMSYDIYGNDTKLWTPPKTERDGFWEGSEWGSGGGLVFRGVFPS 260

Query: 243 KVTADATVCKDGSGGCYGSIQKAVDAAPENAAARRFVIHIKEGVYEEIVRVPLEKKNVVF 302
           K+TAD TVCKDGS GCY ++Q+AV+ AP+N   RRFV+HIKEGVY EIVR+PLEKKNVVF
Sbjct: 261 KLTADVTVCKDGSNGCYKTVQEAVNTAPDNEMGRRFVVHIKEGVYNEIVRIPLEKKNVVF 320

Query: 303 LGDGMGKTVITGSLNAGQPGISTYNTATLGVLGDGFMASGLTIQNTAGPDAHQAVAFRSD 362
           LGDGMGKTVITGSL AGQPG+STYNTAT+GVLGDGFMASGLT QNTAG   HQAVAFRSD
Sbjct: 321 LGDGMGKTVITGSLTAGQPGVSTYNTATVGVLGDGFMASGLTFQNTAGAPTHQAVAFRSD 380

Query: 363 SDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQDCEILIRPRQLK 422
           SDLS I++CEFIG+QDTLYAHSLRQFYKSC+I+GNVDFIFGNSA+IFQDCEI++ PRQ K
Sbjct: 381 SDLSFIENCEFIGHQDTLYAHSLRQFYKSCRIEGNVDFIFGNSAAIFQDCEIVVNPRQEK 440

Query: 423 PEKGENNAITAHGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKVHKNFLGRPWKEYSR 482
           PEKGENNA+TAHGRTDPAQ+TGFVFQNCLINGT+EYM+L++SKP  HKN+LGRPWKEYSR
Sbjct: 441 PEKGENNAVTAHGRTDPAQATGFVFQNCLINGTEEYMALFHSKPGAHKNYLGRPWKEYSR 500

Query: 483 TVFIHCNLEALVSPSGWMPWSGDFALKTLFYGEFENSGVGSKTSERVGWSSQIPAQHVFS 542
            VFIHCN EA+++P GWMPW+GDFALKTL+YGEFENSG GS  S RV WSSQIPA+HV++
Sbjct: 501 VVFIHCNFEAIITPEGWMPWTGDFALKTLYYGEFENSGPGSNLSGRVKWSSQIPAEHVYT 560

Query: 543 YSVQNFIQGDEWIPSS 554
           YSVQNFIQGDEWIP+S
Sbjct: 561 YSVQNFIQGDEWIPTS 574

BLAST of MELO3C023254 vs. NCBI nr
Match: gi|223543610|gb|EEF45139.1| (Pectinesterase-2 precursor, putative [Ricinus communis])

HSP 1 Score: 832.8 bits (2150), Expect = 3.7e-238
Identity = 404/554 (72.92%), Postives = 469/554 (84.66%), Query Frame = 1

Query: 5   SFLSFLSLSFFFFSLSLSSSSSAVRHHHSHSHLP--SHFTNTPSDQILHACKSTRFPDQC 64
           SFLSF  LS + F   LSS+S   RHH     LP  S  + +   +IL ACK+TRFPD C
Sbjct: 3   SFLSFSLLSLYLF---LSSTSVTSRHHTYRPKLPPPSSLSVSTPPEILQACKATRFPDTC 62

Query: 65  GSSLSHSSLKPPQNNPSPLQILQSAIALSSDGLTTAQSLVKQILDSSATNLNRTTAAKNC 124
            SSL+ S   P   NP+PLQI+QS++++S+  L TAQS+VK ILDSSA N+NRT AA+NC
Sbjct: 63  ASSLTSSDSVP--KNPTPLQIIQSSVSISATNLKTAQSMVKSILDSSAGNINRTNAARNC 122

Query: 125 LEVLRNSDYRISLTND-ALHRGRTKDARAWLSASLLYHYDCWSALKYANDTQLVDKTMAF 184
           LEVL NS+YRIS T D +L RGRTKDARAW+SA+LLY YDCWSALKYANDT   +KTM+F
Sbjct: 123 LEVLNNSEYRISSTADNSLPRGRTKDARAWMSAALLYQYDCWSALKYANDTSDTNKTMSF 182

Query: 185 LDSLIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGFWEGG--GGGSGLGFSGGFPAKV 244
           LDSL+ L+SN+LSM+++YDIYGNDT  WTPP+TERDGFWEG   G G GL F G FP+K+
Sbjct: 183 LDSLMLLTSNSLSMVMSYDIYGNDTKLWTPPKTERDGFWEGSEWGSGGGLVFRGVFPSKL 242

Query: 245 TADATVCKDGSGGCYGSIQKAVDAAPENAAARRFVIHIKEGVYEEIVRVPLEKKNVVFLG 304
           TAD TVCKDGS GCY ++Q+AV+ AP+N   RRFV+HIKEGVY EIVR+PLEKKNVVFLG
Sbjct: 243 TADVTVCKDGSNGCYKTVQEAVNTAPDNEMGRRFVVHIKEGVYNEIVRIPLEKKNVVFLG 302

Query: 305 DGMGKTVITGSLNAGQPGISTYNTATLGVLGDGFMASGLTIQNTAGPDAHQAVAFRSDSD 364
           DGMGKTVITGSL AGQPG+STYNTAT+GVLGDGFMASGLT QNTAG   HQAVAFRSDSD
Sbjct: 303 DGMGKTVITGSLTAGQPGVSTYNTATVGVLGDGFMASGLTFQNTAGAPTHQAVAFRSDSD 362

Query: 365 LSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQDCEILIRPRQLKPE 424
           LS I++CEFIG+QDTLYAHSLRQFYKSC+I+GNVDFIFGNSA+IFQDCEI++ PRQ KPE
Sbjct: 363 LSFIENCEFIGHQDTLYAHSLRQFYKSCRIEGNVDFIFGNSAAIFQDCEIVVNPRQEKPE 422

Query: 425 KGENNAITAHGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKVHKNFLGRPWKEYSRTV 484
           KGENNA+TAHGRTDPAQ+TGFVFQNCLINGT+EYM+L++SKP  HKN+LGRPWKEYSR V
Sbjct: 423 KGENNAVTAHGRTDPAQATGFVFQNCLINGTEEYMALFHSKPGAHKNYLGRPWKEYSRVV 482

Query: 485 FIHCNLEALVSPSGWMPWSGDFALKTLFYGEFENSGVGSKTSERVGWSSQIPAQHVFSYS 544
           FIHCN EA+++P GWMPW+GDFALKTL+YGEFENSG GS  S RV WSSQIPA+HV++YS
Sbjct: 483 FIHCNFEAIITPEGWMPWTGDFALKTLYYGEFENSGPGSNLSGRVKWSSQIPAEHVYTYS 542

Query: 545 VQNFIQGDEWIPSS 554
           VQNFIQGDEWIP+S
Sbjct: 543 VQNFIQGDEWIPTS 551