MELO3C022224.2 (gene) Melon (DHL92) v3.6.1

BLAST of MELO3C022224.2 vs. NCBI nr
Match: XP_016902429.1 (PREDICTED: protein NRT1/ PTR FAMILY 5.10-like [Cucumis melo])

HSP 1 Score: 1072.8 bits (2773), Expect = 3.4e-310
Identity = 559/568 (98.42%), Postives = 559/568 (98.42%), Query Frame = 0

Query: 1   MDLIDSMDHATPLLTDMVDGAVDYKGQPLLRSSSATWRSASFIIGMEVAERFAFYGIGAN 60
           MDLIDSMDHATPLLTDMVDGAVDYKGQPLLRSSSATWRSASFIIGMEVAERFAFYGIGAN
Sbjct: 1   MDLIDSMDHATPLLTDMVDGAVDYKGQPLLRSSSATWRSASFIIGMEVAERFAFYGIGAN 60

Query: 61  LITYLTGPLRISVVAAAEIVNVWAGTSMLLTLFGAFLADSFFGRYRTI---------GLG 120
           LITYLTGPLRISVVAAAEIVNVWAGTSMLLTLFGAFLADSFFGRYRTI         GLG
Sbjct: 61  LITYLTGPLRISVVAAAEIVNVWAGTSMLLTLFGAFLADSFFGRYRTIVFASISYILGLG 120

Query: 121 LLVLSTLLPTASSSICAIPNKFAPCSSPKLHLILFFLSLCLIGIAQGGHRPCVQAFGADQ 180
           LLVLSTLLPTASSSICAIPNKFAPCSSPKLHLILFFLSLCLIGIAQGGHRPCVQAFGADQ
Sbjct: 121 LLVLSTLLPTASSSICAIPNKFAPCSSPKLHLILFFLSLCLIGIAQGGHRPCVQAFGADQ 180

Query: 181 FDPQNPQEAKFKSSFFNWWYFGACFGIVAAIPTISYAQENLSWAVGFGIPCVSMVTGFIL 240
           FDPQNPQEAKFKSSFFNWWYFGACFGIVAAIPTISYAQENLSWAVGFGIPCVSMVTGFIL
Sbjct: 181 FDPQNPQEAKFKSSFFNWWYFGACFGIVAAIPTISYAQENLSWAVGFGIPCVSMVTGFIL 240

Query: 241 FLLGTKTYRFNTLKQSDKSPFFRIVRVFVASIRNLRAPASSITFQDEQTAKDLSTSQQFK 300
           FLLGTKTYRFNTLKQSDKSPFFRIVRVFVASIRNLRAPASSITFQDEQTAKDLSTSQQFK
Sbjct: 241 FLLGTKTYRFNTLKQSDKSPFFRIVRVFVASIRNLRAPASSITFQDEQTAKDLSTSQQFK 300

Query: 301 FLNKACIIVPIDSNQTAMTCSISELEEAKAILRILIIWVTVVVFTIAFSQDATFFTKQAA 360
           FLNKACIIVPIDSNQTAMTCSISELEEAKAILRILIIWVTVVVFTIAFSQDATFFTKQAA
Sbjct: 301 FLNKACIIVPIDSNQTAMTCSISELEEAKAILRILIIWVTVVVFTIAFSQDATFFTKQAA 360

Query: 361 TLDRSIMSGFIIPAASLEALVSFTIVIFIVVYDLLFVPIAKKVTGNPSGITTLQRIGTGM 420
           TLDRSIMSGFIIPAASLEALVSFTIVIFIVVYDLLFVPIAKKVTGNPSGITTLQRIGTGM
Sbjct: 361 TLDRSIMSGFIIPAASLEALVSFTIVIFIVVYDLLFVPIAKKVTGNPSGITTLQRIGTGM 420

Query: 421 VISTISMAVASLVEKKRLQTALEHGLIDIPEMTIPMRFWWLVPQYVLNGLADVFTVVGLQ 480
           VISTISMAVASLVEKKRLQTALEHGLIDIPEMTIPMRFWWLVPQYVLNGLADVFTVVGLQ
Sbjct: 421 VISTISMAVASLVEKKRLQTALEHGLIDIPEMTIPMRFWWLVPQYVLNGLADVFTVVGLQ 480

Query: 481 ELCYNQVPKDLKSVGPAIFISILGMGNILSSLLISVIDTATKANGHRSWFSNNLNNAHLD 540
           ELCYNQVPKDLKSVGPAIFISILGMGNILSSLLISVIDTATKANGHRSWFSNNLNNAHLD
Sbjct: 481 ELCYNQVPKDLKSVGPAIFISILGMGNILSSLLISVIDTATKANGHRSWFSNNLNNAHLD 540

Query: 541 YFYLLLAALSVLGFVAFLFVAKSHVYNI 560
           YFYLLLAALSVLGFVAFLFVAKSHVYNI
Sbjct: 541 YFYLLLAALSVLGFVAFLFVAKSHVYNI 568

BLAST of MELO3C022224.2 vs. NCBI nr
Match: XP_011656124.1 (PREDICTED: protein NRT1/ PTR FAMILY 5.10-like [Cucumis sativus])

HSP 1 Score: 1028.1 bits (2657), Expect = 1.1e-296
Identity = 533/567 (94.00%), Postives = 546/567 (96.30%), Query Frame = 0

Query: 1   MDLIDSMDHATPLLTDMVDGAVDYKGQPLLRSSSATWRSASFIIGMEVAERFAFYGIGAN 60
           MDLIDSMDHATPLLTDMVDGAVDYKGQPLLRSSSATWRSASFIIGMEVAERF+FYGIGAN
Sbjct: 1   MDLIDSMDHATPLLTDMVDGAVDYKGQPLLRSSSATWRSASFIIGMEVAERFSFYGIGAN 60

Query: 61  LITYLTGPLRISVVAAAEIVNVWAGTSMLLTLFGAFLADSFFGRYRTI---------GLG 120
           LITYLTGPL ISVVAAAEIVNVWA TSMLLTLFGAFLADSFFGRYRTI         GLG
Sbjct: 61  LITYLTGPLGISVVAAAEIVNVWAATSMLLTLFGAFLADSFFGRYRTIVFASISYILGLG 120

Query: 121 LLVLSTLLPTASSSICAIPNKFAPCSSPKLHLILFFLSLCLIGIAQGGHRPCVQAFGADQ 180
           LLVLSTLLPT SSSICAI NKF PCSSPKLHLILFFLSLCLIGIAQGGHRPCVQAFGADQ
Sbjct: 121 LLVLSTLLPTPSSSICAILNKFTPCSSPKLHLILFFLSLCLIGIAQGGHRPCVQAFGADQ 180

Query: 181 FDPQNPQEAKFKSSFFNWWYFGACFGIVAAIPTISYAQENLSWAVGFGIPCVSMVTGFIL 240
           FDPQNPQEAKFKSSFFNWWYFGACFGIVAAIPT+SYAQENLSWAVGFGIPCVSM+TGFIL
Sbjct: 181 FDPQNPQEAKFKSSFFNWWYFGACFGIVAAIPTVSYAQENLSWAVGFGIPCVSMLTGFIL 240

Query: 241 FLLGTKTYRFNTLKQSDKSPFFRIVRVFVASIRNLRAPASSITFQDEQTAKDLSTSQQFK 300
           FLLGT+TYRFNTLKQSDKSPF+RI RVF+ASIRNLRA AS+ITFQDEQTAK LSTSQQFK
Sbjct: 241 FLLGTETYRFNTLKQSDKSPFWRIGRVFLASIRNLRASASTITFQDEQTAKYLSTSQQFK 300

Query: 301 FLNKACIIVPIDSNQTAMTCSISELEEAKAILRILIIWVTVVVFTIAFSQDATFFTKQAA 360
           FLNKACIIVPIDSNQ AMTCS+SELEEAKAILRILIIWVTVVVFTIAFSQDATFFTKQAA
Sbjct: 301 FLNKACIIVPIDSNQNAMTCSVSELEEAKAILRILIIWVTVVVFTIAFSQDATFFTKQAA 360

Query: 361 TLDRSIMSGFIIPAASLEALVSFTIVIFIVVYDLLFVPIAKKVTGNPSGITTLQRIGTGM 420
           TLDRSIMSGFIIPAASLEAL+SFTIVIFIVVYDLLFVPIAKKVTGNPSGITTLQRIGTGM
Sbjct: 361 TLDRSIMSGFIIPAASLEALISFTIVIFIVVYDLLFVPIAKKVTGNPSGITTLQRIGTGM 420

Query: 421 VISTISMAVASLVEKKRLQTALEHGLIDIPEMTIPMRFWWLVPQYVLNGLADVFTVVGLQ 480
           VISTISMAVASLVEKKRL+TAL+HGL+D PEMTIPMRFWWLVPQYVLNGLADVFTVVGLQ
Sbjct: 421 VISTISMAVASLVEKKRLKTALDHGLVDTPEMTIPMRFWWLVPQYVLNGLADVFTVVGLQ 480

Query: 481 ELCYNQVPKDLKSVGPAIFISILGMGNILSSLLISVIDTATKANGHRSWFSNNLNNAHLD 540
           ELCY+QVPKDLKSVGPAIFISILGMGNILSSLLISVIDTATKANGHRSWFSNNLN AHLD
Sbjct: 481 ELCYDQVPKDLKSVGPAIFISILGMGNILSSLLISVIDTATKANGHRSWFSNNLNKAHLD 540

Query: 541 YFYLLLAALSVLGFVAFLFVAKSHVYN 559
           YFYLLLAALSV GFVAFLFVAKSHVYN
Sbjct: 541 YFYLLLAALSVFGFVAFLFVAKSHVYN 567

BLAST of MELO3C022224.2 vs. NCBI nr
Match: KGN52718.1 (hypothetical protein Csa_5G652240 [Cucumis sativus])

HSP 1 Score: 1017.3 bits (2629), Expect = 2.0e-293
Identity = 533/584 (91.27%), Postives = 546/584 (93.49%), Query Frame = 0

Query: 1   MDLIDSMDHATPLLTDMVDGAVDYKGQPLLRSSSATWRSASFIIGMEVAERFAFYGIGAN 60
           MDLIDSMDHATPLLTDMVDGAVDYKGQPLLRSSSATWRSASFIIGMEVAERF+FYGIGAN
Sbjct: 1   MDLIDSMDHATPLLTDMVDGAVDYKGQPLLRSSSATWRSASFIIGMEVAERFSFYGIGAN 60

Query: 61  LITYLTGPLRISVVAAAEIVNVWAGTSMLLTLFGAFLADSFFGRYRTI---------GLG 120
           LITYLTGPL ISVVAAAEIVNVWA TSMLLTLFGAFLADSFFGRYRTI         GLG
Sbjct: 61  LITYLTGPLGISVVAAAEIVNVWAATSMLLTLFGAFLADSFFGRYRTIVFASISYILGLG 120

Query: 121 LLVLSTLLPTASSSICAIPNKFAPCSSPKLHLILFFLSLCLIGIAQGGHRPCVQAFGADQ 180
           LLVLSTLLPT SSSICAI NKF PCSSPKLHLILFFLSLCLIGIAQGGHRPCVQAFGADQ
Sbjct: 121 LLVLSTLLPTPSSSICAILNKFTPCSSPKLHLILFFLSLCLIGIAQGGHRPCVQAFGADQ 180

Query: 181 FDPQNPQEAKFKSSFFNWWYFGACFGIVAAIPTISYAQENLSWAVGFGIPCVSMVTGFIL 240
           FDPQNPQEAKFKSSFFNWWYFGACFGIVAAIPT+SYAQENLSWAVGFGIPCVSM+TGFIL
Sbjct: 181 FDPQNPQEAKFKSSFFNWWYFGACFGIVAAIPTVSYAQENLSWAVGFGIPCVSMLTGFIL 240

Query: 241 FLLGTKTYRFNTLKQSDKSPFFRIVRVFVASIRNLRAPASSITFQDEQTAKDLSTSQQFK 300
           FLLGT+TYRFNTLKQSDKSPF+RI RVF+ASIRNLRA AS+ITFQDEQTAK LSTSQQFK
Sbjct: 241 FLLGTETYRFNTLKQSDKSPFWRIGRVFLASIRNLRASASTITFQDEQTAKYLSTSQQFK 300

Query: 301 -----------------FLNKACIIVPIDSNQTAMTCSISELEEAKAILRILIIWVTVVV 360
                            FLNKACIIVPIDSNQ AMTCS+SELEEAKAILRILIIWVTVVV
Sbjct: 301 MHIMPTEDASFHVWCDRFLNKACIIVPIDSNQNAMTCSVSELEEAKAILRILIIWVTVVV 360

Query: 361 FTIAFSQDATFFTKQAATLDRSIMSGFIIPAASLEALVSFTIVIFIVVYDLLFVPIAKKV 420
           FTIAFSQDATFFTKQAATLDRSIMSGFIIPAASLEAL+SFTIVIFIVVYDLLFVPIAKKV
Sbjct: 361 FTIAFSQDATFFTKQAATLDRSIMSGFIIPAASLEALISFTIVIFIVVYDLLFVPIAKKV 420

Query: 421 TGNPSGITTLQRIGTGMVISTISMAVASLVEKKRLQTALEHGLIDIPEMTIPMRFWWLVP 480
           TGNPSGITTLQRIGTGMVISTISMAVASLVEKKRL+TAL+HGL+D PEMTIPMRFWWLVP
Sbjct: 421 TGNPSGITTLQRIGTGMVISTISMAVASLVEKKRLKTALDHGLVDTPEMTIPMRFWWLVP 480

Query: 481 QYVLNGLADVFTVVGLQELCYNQVPKDLKSVGPAIFISILGMGNILSSLLISVIDTATKA 540
           QYVLNGLADVFTVVGLQELCY+QVPKDLKSVGPAIFISILGMGNILSSLLISVIDTATKA
Sbjct: 481 QYVLNGLADVFTVVGLQELCYDQVPKDLKSVGPAIFISILGMGNILSSLLISVIDTATKA 540

Query: 541 NGHRSWFSNNLNNAHLDYFYLLLAALSVLGFVAFLFVAKSHVYN 559
           NGHRSWFSNNLN AHLDYFYLLLAALSV GFVAFLFVAKSHVYN
Sbjct: 541 NGHRSWFSNNLNKAHLDYFYLLLAALSVFGFVAFLFVAKSHVYN 584

BLAST of MELO3C022224.2 vs. NCBI nr
Match: XP_023535819.1 (protein NRT1/ PTR FAMILY 5.10-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 884.0 bits (2283), Expect = 2.6e-253
Identity = 459/561 (81.82%), Postives = 502/561 (89.48%), Query Frame = 0

Query: 7   MDHATPLLTDMVDGAVDYKGQPLLRSSSATWRSASFIIGMEVAERFAFYGIGANLITYLT 66
           MDH  PLL+  VDGAVDYKG+PLLRSSS TW SASFIIGMEVAERFAFYGIGANLITYLT
Sbjct: 1   MDHTAPLLSHTVDGAVDYKGRPLLRSSSGTWSSASFIIGMEVAERFAFYGIGANLITYLT 60

Query: 67  GPLRISVVAAAEIVNVWAGTSMLLTLFGAFLADSFFGRYRTI---------GLGLLVLST 126
           GPL IS+VAAAEIVNVW+GTSMLLTLFGAF+ADSFFGRYRTI         GLGLLVLS 
Sbjct: 61  GPLHISMVAAAEIVNVWSGTSMLLTLFGAFIADSFFGRYRTIVFASASYLLGLGLLVLS- 120

Query: 127 LLPTASSSICAIPNKFAPCSSPKLHLILFFLSLCLIGIAQGGHRPCVQAFGADQFDPQNP 186
              TA+SSICA+P+K   CS+PKL L+LFF SLCLIGIAQGGHRPCVQAFGADQFD QNP
Sbjct: 121 ---TANSSICAVPDKSTTCSAPKLQLVLFFASLCLIGIAQGGHRPCVQAFGADQFDDQNP 180

Query: 187 QEAKFKSSFFNWWYFGACFGIVAAIPTISYAQENLSWAVGFGIPCVSMVTGFILFLLGTK 246
           QEAKFKSSFFNWWYFGAC GIV AIPTI+YAQENLSW+VGFGIP  +M+TGFI+FLLGTK
Sbjct: 181 QEAKFKSSFFNWWYFGACSGIVVAIPTIAYAQENLSWSVGFGIPSAAMLTGFIVFLLGTK 240

Query: 247 TYRFNTLKQSDKSPFFRIVRVFVASIRNLRAPASSITFQDEQTAKDLSTSQQFKFLNKAC 306
           TYRF T+KQSDKSPF RI RVFVASIRN RA  S+I+F +E+T KD+S SQQFKFLNKAC
Sbjct: 241 TYRF-TVKQSDKSPFMRIGRVFVASIRNWRASPSTISFNEEETGKDVSNSQQFKFLNKAC 300

Query: 307 IIVPIDSNQTAMTCSISELEEAKAILRILIIWVTVVVFTIAFSQDATFFTKQAATLDRSI 366
            IVPIDSNQ A  CSISE+EEAKAILRIL+IW T+++FTI FSQDATFFTKQAATLDR+I
Sbjct: 301 -IVPIDSNQNATACSISEVEEAKAILRILLIWPTLIMFTIVFSQDATFFTKQAATLDRTI 360

Query: 367 MSGFIIPAASLEALVSFTIVIFIVVYDLLFVPIAKKVTGNPSGITTLQRIGTGMVISTIS 426
            SGF +PAASLEA++SFTIVIFIVVYDL+FVPIAK VTGN SGITTLQRIG+GM+ISTIS
Sbjct: 361 SSGFTVPAASLEAIISFTIVIFIVVYDLVFVPIAKTVTGNSSGITTLQRIGSGMIISTIS 420

Query: 427 MAVASLVEKKRLQTALEHGLIDIPEMTIPMRFWWLVPQYVLNGLADVFTVVGLQELCYNQ 486
           M VASLVEKKRL+TALEHGLID P+MTIPM+F WLVPQYVLNGLADVFTVVGLQE CY+Q
Sbjct: 421 MVVASLVEKKRLKTALEHGLIDRPDMTIPMKFGWLVPQYVLNGLADVFTVVGLQEFCYDQ 480

Query: 487 VPKDLKSVGPAIFISILGMGNILSSLLISVIDTATKANGHRSWFSNNLNNAHLDYFYLLL 546
           V +DLKSVGPAIFISILGMG+ILSSLLIS+ID+ATK NGH SWF +NLN AHLDYFYLLL
Sbjct: 481 VARDLKSVGPAIFISILGMGSILSSLLISIIDSATKGNGHLSWFPDNLNKAHLDYFYLLL 540

Query: 547 AALSVLGFVAFLFVAKSHVYN 559
           AALSVL F+AFLFVAKSHVYN
Sbjct: 541 AALSVLSFIAFLFVAKSHVYN 555

BLAST of MELO3C022224.2 vs. NCBI nr
Match: XP_022925412.1 (uncharacterized protein LOC111432711 isoform X2 [Cucurbita moschata])

HSP 1 Score: 873.6 bits (2256), Expect = 3.5e-250
Identity = 454/558 (81.36%), Postives = 495/558 (88.71%), Query Frame = 0

Query: 10   ATPLLTDMVDGAVDYKGQPLLRSSSATWRSASFIIGMEVAERFAFYGIGANLITYLTGPL 69
            +TPLL+  VDGAVDYKG+PLLRSSS TW SASFIIGMEVAERFAFYGIGANLITYLTGPL
Sbjct: 543  STPLLSHTVDGAVDYKGRPLLRSSSGTWSSASFIIGMEVAERFAFYGIGANLITYLTGPL 602

Query: 70   RISVVAAAEIVNVWAGTSMLLTLFGAFLADSFFGRYRTI---------GLGLLVLSTLLP 129
             IS+VAAAEIVNVW+GTSMLLTLFGAF+ADSFFGRYRTI         GLGLLVLS    
Sbjct: 603  HISMVAAAEIVNVWSGTSMLLTLFGAFVADSFFGRYRTIVFASISYLLGLGLLVLS---- 662

Query: 130  TASSSICAIPNKFAPCSSPKLHLILFFLSLCLIGIAQGGHRPCVQAFGADQFDPQNPQEA 189
            TA+SSICA+PNK  PCS+PKL L+LFF SLCLIGIAQGGHRPCVQAFGADQFD QNPQEA
Sbjct: 663  TANSSICAVPNKSMPCSAPKLQLVLFFASLCLIGIAQGGHRPCVQAFGADQFDDQNPQEA 722

Query: 190  KFKSSFFNWWYFGACFGIVAAIPTISYAQENLSWAVGFGIPCVSMVTGFILFLLGTKTYR 249
            KFK SFFNWWYFGAC GIV AIPT++YAQENLSW+VGFGIPC +M+TGFI+FLLGTKTYR
Sbjct: 723  KFKGSFFNWWYFGACSGIVVAIPTVAYAQENLSWSVGFGIPCAAMLTGFIVFLLGTKTYR 782

Query: 250  FNTLKQSDKSPFFRIVRVFVASIRNLRAPASSITFQDEQTAKDLSTSQQFKFLNKACIIV 309
            F T+KQSDKSPF R+ RVFVASIRN RA  S I        KDLS SQQFKFLNKAC IV
Sbjct: 783  F-TVKQSDKSPFMRVGRVFVASIRNWRASPSRI-------GKDLSNSQQFKFLNKAC-IV 842

Query: 310  PIDSNQTAMTCSISELEEAKAILRILIIWVTVVVFTIAFSQDATFFTKQAATLDRSIMSG 369
            PIDSNQ A  CSISE+EEAKAILRIL+IW T+++FTI FSQDATFFTKQAATLDR+I SG
Sbjct: 843  PIDSNQNATACSISEVEEAKAILRILLIWATLIMFTIVFSQDATFFTKQAATLDRTISSG 902

Query: 370  FIIPAASLEALVSFTIVIFIVVYDLLFVPIAKKVTGNPSGITTLQRIGTGMVISTISMAV 429
            F +PAASLEA++SFTIVIFIVVYDL+FVPIAK VTGN SGITTLQRIG+GM+ISTISM V
Sbjct: 903  FTVPAASLEAIISFTIVIFIVVYDLVFVPIAKTVTGNSSGITTLQRIGSGMIISTISMVV 962

Query: 430  ASLVEKKRLQTALEHGLIDIPEMTIPMRFWWLVPQYVLNGLADVFTVVGLQELCYNQVPK 489
            ASLVEKKRL+TALEHGLID P+MTIPM+FWWLVPQYVLNGLADVFTVVGLQE CY+QV +
Sbjct: 963  ASLVEKKRLKTALEHGLIDRPDMTIPMKFWWLVPQYVLNGLADVFTVVGLQEFCYDQVAR 1022

Query: 490  DLKSVGPAIFISILGMGNILSSLLISVIDTATKANGHRSWFSNNLNNAHLDYFYLLLAAL 549
            DLKSVGPAIFISILGMG+ILSSLLIS+ID+ATK NGH SWF +NLN AHLDYFYLLL+ L
Sbjct: 1023 DLKSVGPAIFISILGMGSILSSLLISIIDSATKGNGHLSWFPDNLNKAHLDYFYLLLSGL 1082

Query: 550  SVLGFVAFLFVAKSHVYN 559
            SVL F+AFLFVAKSHVYN
Sbjct: 1083 SVLSFIAFLFVAKSHVYN 1087

BLAST of MELO3C022224.2 vs. TAIR10
Match: AT1G22540.1 (Major facilitator superfamily protein)

HSP 1 Score: 584.7 bits (1506), Expect = 6.0e-167
Identity = 304/567 (53.62%), Postives = 406/567 (71.60%), Query Frame = 0

Query: 1   MDLIDSMDHA-TPLLTDMVDGAVDYKGQPLLRSSSATWRSASFIIGMEVAERFAFYGIGA 60
           M +  ++D A TPLL       VDY+ +P ++SSS  WRSA FIIG+EVAERFA+YGI +
Sbjct: 1   MSISGAVDEAGTPLLA----VTVDYRNKPAVKSSSGGWRSAGFIIGVEVAERFAYYGISS 60

Query: 61  NLITYLTGPLRISVVAAAEIVNVWAGTSMLLTLFGAFLADSFFGRYRTI---------GL 120
           NLITYLTGPL  S  AAA  VN W+GT+ LL L GAF+ADSF GR+RTI         GL
Sbjct: 61  NLITYLTGPLGQSTAAAAANVNAWSGTASLLPLLGAFVADSFLGRFRTILAASALYIVGL 120

Query: 121 GLLVLSTLLPTASSSICAIPNKFAPCSSPKLHLILFFLSLCLIGIAQGGHRPCVQAFGAD 180
           G+L LS ++P    S C + N  + C SP+  +I FF +L L+ +AQGGH+PCVQAFGAD
Sbjct: 121 GVLTLSAMIP----SDCKVSNLLSSC-SPRFQVITFFSALYLVALAQGGHKPCVQAFGAD 180

Query: 181 QFDPQNPQEAKFKSSFFNWWYFGACFGIVAAIPTISYAQENLSWAVGFGIPCVSMVTGFI 240
           QFD + P+E K KSSFFNWWYFG CFG +  +  ++Y Q+NLSWA+GFGIPC++MV   +
Sbjct: 181 QFDEKEPEECKAKSSFFNWWYFGMCFGTLTTLWVLNYIQDNLSWALGFGIPCIAMVVALV 240

Query: 241 LFLLGTKTYRFNTLKQSDKSPFFRIVRVFVASIRNLRAPASSITFQDEQTA-KDLSTSQQ 300
           + LLGT TYRF ++++ D+SPF RI  V+VA+++N    A  +   +E+      S+SQQ
Sbjct: 241 VLLLGTCTYRF-SIRREDQSPFVRIGNVYVAAVKNWSVSALDVAAAEERLGLVSCSSSQQ 300

Query: 301 FKFLNKACIIVPIDSNQTAMTCSISELEEAKAILRILIIWVTVVVFTIAFSQDATFFTKQ 360
           F FLNKA +           +CSI ELEEAK++LR+  IW+T +V+ + F+Q  TFFTKQ
Sbjct: 301 FSFLNKALV-------AKNGSCSIDELEEAKSVLRLAPIWLTCLVYAVVFAQSPTFFTKQ 360

Query: 361 AATLDRSIMSGFIIPAASLEALVSFTIVIFIVVYDLLFVPIAKKVTGNPSGITTLQRIGT 420
            AT++RSI  G+ I  A+L++ +S +IVIFI +YD + +PIA+  T  P GIT LQRIGT
Sbjct: 361 GATMERSITPGYKISPATLQSFISLSIVIFIPIYDRVLIPIARSFTHKPGGITMLQRIGT 420

Query: 421 GMVISTISMAVASLVEKKRLQTALEHGLIDIPEMTIPMRFWWLVPQYVLNGLADVFTVVG 480
           G+ +S ++M VA+LVE KRL+TA ++GL+D P+ T+PM  WWLVPQYVL G+ DVF +VG
Sbjct: 421 GIFLSFLAMVVAALVEMKRLKTAADYGLVDSPDATVPMSVWWLVPQYVLFGITDVFAMVG 480

Query: 481 LQELCYNQVPKDLKSVGPAIFISILGMGNILSSLLISVIDTATKANGHRSWFSNNLNNAH 540
           LQE  Y+QVP +L+SVG A+++SI G+GN LSS +IS+I+ AT  +G  SWF+NNLN AH
Sbjct: 481 LQEFFYDQVPNELRSVGLALYLSIFGIGNFLSSFMISIIEKATSQSGQASWFANNLNQAH 540

Query: 541 LDYFYLLLAALSVLGFVAFLFVAKSHV 557
           LDYFY LLA LS +G  ++L+VAKS+V
Sbjct: 541 LDYFYWLLACLSFIGLASYLYVAKSYV 550

BLAST of MELO3C022224.2 vs. TAIR10
Match: AT1G72125.1 (Major facilitator superfamily protein)

HSP 1 Score: 540.8 bits (1392), Expect = 9.9e-154
Identity = 283/550 (51.45%), Postives = 386/550 (70.18%), Query Frame = 0

Query: 11  TPLLTDMVDGAVDYKGQPLLRSSSATWRSASFIIGMEVAERFAFYGIGANLITYLTGPLR 70
           T L  + V  AVD++G    RS +  WR+A FIIG+EVAERFA YGIG+NLI+YLTGPL 
Sbjct: 7   TSLQEEYVIDAVDHRGFSARRSITGRWRAAWFIIGVEVAERFANYGIGSNLISYLTGPLG 66

Query: 71  ISVVAAAEIVNVWAGTSMLLTLFGAFLADSFFGRYRTIGLGLLVLSTLLPTASSSICAIP 130
            S   AA  VN W+G S +L L GAF+AD+F GRY TI +   +    L   + S   IP
Sbjct: 67  QSTAVAAANVNAWSGISTILPLLGAFVADAFLGRYITIIIASFIYVLGLAFLTLSAFLIP 126

Query: 131 NKFAPCSSPKLHL-ILFFLSLCLIGIAQGGHRPCVQAFGADQFDPQNPQEAKFKSSFFNW 190
           N     SSP   L  LFF SL L+ I Q GH+PCVQAFGADQFD +NPQE   +SSFFNW
Sbjct: 127 NNTEVTSSPSSFLNALFFFSLYLVAIGQSGHKPCVQAFGADQFDEKNPQENSDRSSFFNW 186

Query: 191 WYFGACFGIVAAIPTISYAQENLSWAVGFGIPCVSMVTGFILFLLGTKTYRFNTLKQSDK 250
           WY   C GI  AI  + Y QEN+SWA+GFGIPCV MV   +LF+LG K+YRF+  +Q ++
Sbjct: 187 WYLSMCAGIGLAILVVVYIQENVSWALGFGIPCVFMVISLVLFVLGRKSYRFSKTRQEEE 246

Query: 251 -SPFFRIVRVFVASIRNLRAPASSI-TFQDEQTAKDLSTSQQFKFLNKACIIVPIDSNQT 310
            +PF RI RVF  + +N R  +S +   +  +  +   + ++  FLNKA ++VP DS++ 
Sbjct: 247 TNPFTRIGRVFFVAFKNQRLNSSDLCKVELIEANRSQESPEELSFLNKA-LLVPNDSDEG 306

Query: 311 AMTCSISELEEAKAILRILIIWVTVVVFTIAFSQDATFFTKQAATLDRSIMSGFIIPAAS 370
            + C   ++E+A A++R++ +W+T + + I F+Q  TFFTKQ  T++R+I  G  IP AS
Sbjct: 307 EVACKSRDVEDATALVRLIPVWLTTLAYAIPFAQYMTFFTKQGVTMERTIFPGVEIPPAS 366

Query: 371 LEALVSFTIVIFIVVYDLLFVPIAKKVTGNPSGITTLQRIGTGMVISTISMAVASLVEKK 430
           L+ L+S +IV+F+ +YD + VPI + +T +P GITTL+RIGTGMV++T++M VA+LVE K
Sbjct: 367 LQVLISISIVLFVPIYDRVLVPIGRSITKDPCGITTLKRIGTGMVLATLTMVVAALVESK 426

Query: 431 RLQTALEHGLIDIPEMTIPMRFWWLVPQYVLNGLADVFTVVGLQELCYNQVPKDLKSVGP 490
           RL+TA E+GLID P+ T+PM  WWL PQY+L GLADV T+VG+QE  Y+QVP +L+S+G 
Sbjct: 427 RLETAKEYGLIDQPKTTLPMSIWWLFPQYMLLGLADVHTLVGMQEFFYSQVPTELRSLGL 486

Query: 491 AIFISILGMGNILSSLLISVIDTATKANGHRSWFSNNLNNAHLDYFYLLLAALSVLGFVA 550
           AI++S +G+G++LSSLLI +ID AT  +   SWF++NLN AHLDYFY LLA +S +GF  
Sbjct: 487 AIYLSAMGVGSLLSSLLIYLIDLATGGDAGNSWFNSNLNRAHLDYFYWLLAVVSAVGFFT 546

Query: 551 FLFVAKSHVY 558
           FLF++KS++Y
Sbjct: 547 FLFISKSYIY 555

BLAST of MELO3C022224.2 vs. TAIR10
Match: AT1G72120.1 (Major facilitator superfamily protein)

HSP 1 Score: 531.2 bits (1367), Expect = 7.8e-151
Identity = 279/548 (50.91%), Postives = 383/548 (69.89%), Query Frame = 0

Query: 13  LLTDMVDGAVDYKGQPLLRSSSATWRSASFIIGMEVAERFAFYGIGANLITYLTGPLRIS 72
           L  + V  AVD++G    RS++  WR+A FIIG+EVAERFA+YGIG+NLI+YLTGPL  S
Sbjct: 9   LQEEYVTDAVDHRGLAARRSNTGRWRAALFIIGVEVAERFAYYGIGSNLISYLTGPLGES 68

Query: 73  VVAAAEIVNVWAGTSMLLTLFGAFLADSFFGRYRTIGLGLLVLSTLLPTASSSICAIPNK 132
              AA  VN W+G + LL + GAF+AD+F GRYRTI +  L+    L   + S   IPN 
Sbjct: 69  TAVAAANVNAWSGIATLLPVLGAFVADAFLGRYRTIIISSLIYVLGLAFLTLSAFLIPNT 128

Query: 133 FAPCSSPKLHL-ILFFLSLCLIGIAQGGHRPCVQAFGADQFDPQNPQEAKFKSSFFNWWY 192
               SS    L +LFF SL L+ I Q GH+PCVQAFGADQFD ++ QE   +SSFFNWWY
Sbjct: 129 TEVTSSTSSFLNVLFFFSLYLVAIGQSGHKPCVQAFGADQFDEKDSQEKSDRSSFFNWWY 188

Query: 193 FGACFGIVAAIPTISYAQENLSWAVGFGIPCVSMVTGFILFLLGTKTYRFNTLKQSDK-S 252
                GI  AI  + Y QE  SWA GFGIPCV MV   +LF+ G + YR++  +  ++ +
Sbjct: 189 LSLSAGICFAILVVVYIQEEFSWAFGFGIPCVFMVISLVLFVSGRRIYRYSKRRHEEEIN 248

Query: 253 PFFRIVRVFVASIRNLRAPASSITFQDEQTAKDLSTS-QQFKFLNKACIIVPIDSNQTAM 312
           PF RI RVF  +++N R  +S +     +   + +TS ++  F NKA ++VP DS+Q   
Sbjct: 249 PFTRIGRVFFVALKNQRLSSSDLC----KVELEANTSPEKQSFFNKA-LLVPNDSSQGEN 308

Query: 313 TCSISELEEAKAILRILIIWVTVVVFTIAFSQDATFFTKQAATLDRSIMSGFIIPAASLE 372
               S++E+A A++R++ +W T + + I ++Q  TFFTKQ  T+DR+I+ G  IP ASL+
Sbjct: 309 ASKSSDVEDATALIRLIPVWFTTLAYAIPYAQYMTFFTKQGVTMDRTILPGVKIPPASLQ 368

Query: 373 ALVSFTIVIFIVVYDLLFVPIAKKVTGNPSGITTLQRIGTGMVISTISMAVASLVEKKRL 432
             +  +IV+F+ +YD +FVPIA+ +T  P GITTL+RIGTG+V+STI+M +A+LVE KRL
Sbjct: 369 VFIGISIVLFVPIYDRVFVPIARLITKEPCGITTLKRIGTGIVLSTITMVIAALVEFKRL 428

Query: 433 QTALEHGLIDIPEMTIPMRFWWLVPQYVLNGLADVFTVVGLQELCYNQVPKDLKSVGPAI 492
           +TA EHGLID PE T+PM  WWL+PQY+L GLADV+T+VG+QE  Y+QVP +L+S+G A+
Sbjct: 429 ETAKEHGLIDQPEATLPMSIWWLIPQYLLLGLADVYTLVGMQEFFYSQVPTELRSIGLAL 488

Query: 493 FISILGMGNILSSLLISVIDTATKANGHRSWFSNNLNNAHLDYFYLLLAALSVLGFVAFL 552
           ++S LG+G++LSSLLIS+ID AT  +   SWF++NLN AHLDYFY LLA +S +GF  FL
Sbjct: 489 YLSALGVGSLLSSLLISLIDLATGGDAGNSWFNSNLNRAHLDYFYWLLAIVSAVGFFTFL 548

Query: 553 FVAKSHVY 558
           F++KS++Y
Sbjct: 549 FISKSYIY 551

BLAST of MELO3C022224.2 vs. TAIR10
Match: AT1G72140.1 (Major facilitator superfamily protein)

HSP 1 Score: 504.6 bits (1298), Expect = 7.8e-143
Identity = 276/557 (49.55%), Postives = 373/557 (66.97%), Query Frame = 0

Query: 18  VDGAVDYKGQPLLRSSSATWRSASFIIGMEVAERFAFYGIGANLITYLTGPLRISVVAAA 77
           V+ +VD++G P +RSSS  W+S+ F +  EVAE+FA++GI +NLITY T  L  S   AA
Sbjct: 19  VEFSVDFRGNPSIRSSSGAWKSSGFTMCAEVAEKFAYFGIASNLITYFTEALGESTAVAA 78

Query: 78  EIVNVWAGTSMLLTLFGAFLADSFFGRYRTI---------GLGLLVLSTLLPTASSSICA 137
             VN+W GT+  L L    +ADSF GR+RTI         GLGLL  S  +P    S+C 
Sbjct: 79  SNVNLWLGTAAFLPLIWGSIADSFLGRFRTILLTSSFYIMGLGLLTFSATIP----SLCN 138

Query: 138 IPNKFAPCSSPKLHLILFFLSLCLIGIAQGGHRPCVQAFGADQFDPQNPQEAKFKSSFFN 197
                  C S ++ +I+FF +L LI + +GG + C++AFGADQFD Q+P E+K KSS+FN
Sbjct: 139 DQETRESCVS-QVKVIIFFCALYLIALGEGGFKVCLRAFGADQFDEQDPNESKAKSSYFN 198

Query: 198 WWYFGACFGIVAAIPTISYAQENLSWAVGFGIPCVSMVTGFILFLLGTKTYRFNT---LK 257
           W YF    GI+      +Y QENLSWA+G+ IPC+SM+    LFLLG KTYRF+T    +
Sbjct: 199 WLYFAISIGILTTRLVTNYVQENLSWALGYAIPCLSMMLALFLFLLGIKTYRFSTGGEGR 258

Query: 258 QSDK--SPFFRIVRVFVASIRNLRAPASS--ITFQDEQTAKDLSTSQQFKFLNKACIIVP 317
           Q  K  +PF RI RVFVA+ RN R   S   +   +E T K       F+FL++A I   
Sbjct: 259 QGKKHDNPFVRIGRVFVAAARNRRQTPSDTCLLLPNESTKK-------FRFLDRAVI--- 318

Query: 318 IDSNQTAMTCSISELEEAKAILRILIIWVTVVVFTIAFSQDATFFTKQAATLDRSIMSGF 377
                   +C   E+EEAKA+L ++ IW+  +VF I F+Q  TFFTKQ +T+DRSI S  
Sbjct: 319 --------SCDSYEVEEAKAVLSLIPIWLCSLVFGIVFAQSPTFFTKQGSTMDRSISSTL 378

Query: 378 IIPAASLEALVSFTIVIFIVVYDLLFVPIAKKVTGNPSGITTLQRIGTGMVISTISMAVA 437
            +PAA+L+  +S  I++FI +YD LFVPIA+ +T  P+GITTLQRI TG+ +S ISM +A
Sbjct: 379 QVPAATLQCFISLAILVFIPIYDRLFVPIARSITRKPAGITTLQRISTGIFLSIISMVIA 438

Query: 438 SLVEKKRLQTALEHGLIDIPEMTIPMRFWWLVPQYVLNGLADVFTVVGLQELCYNQVPKD 497
           +LVE KRL+TA +HGL+D P+ T+PM   WL+PQY+L G++DVFT+VGLQE  Y +VP  
Sbjct: 439 ALVEMKRLKTARDHGLVDSPKATVPMSVCWLIPQYILFGVSDVFTMVGLQEFFYGEVPPQ 498

Query: 498 LKSVGPAIFISILGMGNILSSLLISVIDTATKANGHRSWFSNNLNNAHLDYFYLLLAALS 557
           L+S+G A+++SI+G+GN LSS ++SVI+ AT  +G  SWFSNNLN AHLDYFY LLA LS
Sbjct: 499 LRSMGLALYLSIIGIGNFLSSFMVSVIEEATSQSGQVSWFSNNLNQAHLDYFYWLLACLS 552

Query: 558 VLGFVAFLFVAKSHVYN 559
            L F+  ++ AKS++YN
Sbjct: 559 SLAFIFTVYFAKSYLYN 552

BLAST of MELO3C022224.2 vs. TAIR10
Match: AT1G22550.1 (Major facilitator superfamily protein)

HSP 1 Score: 496.5 bits (1277), Expect = 2.1e-140
Identity = 269/556 (48.38%), Postives = 372/556 (66.91%), Query Frame = 0

Query: 13  LLTDMVDGAVDYKGQPLLRSSSATWRSASFIIGMEVAERFAFYGIGANLITYLTGPLRIS 72
           L+ D V  +VD++G P  +SS+  WRSA +IIG+EV ERFA++GIG+NLITYLTGPL  S
Sbjct: 10  LIEDSVSDSVDHRGLPAGKSSTGGWRSAWYIIGVEVGERFAYFGIGSNLITYLTGPLGQS 69

Query: 73  VVAAAEIVNVWAGTSMLLTLFGAFLADSFFGRYRTI---------GLGLLVLSTLLPTAS 132
              AA  VN W+GT+ +L + GAF+AD++ GRYRTI         GLGLL LS++L    
Sbjct: 70  TATAAVNVNTWSGTASILPVLGAFIADAYLGRYRTIVVASLIYILGLGLLTLSSILILMG 129

Query: 133 SSICAIPNKFAPCSSPKLHLILFFLSLCLIGIAQGGHRPCVQAFGADQFDPQNPQEAKFK 192
            S     N+ A         ILFF SL L+ I QGGH+PCVQAFGADQFD  +P+E   +
Sbjct: 130 LSEQRQHNRNASAKPFFWVNILFFCSLYLVAIGQGGHKPCVQAFGADQFDVGDPKERISR 189

Query: 193 SSFFNWWYFGACFGIVAAIPTISYAQENLSWAVGFGIPCVSMVTGFILFLLGTKTYRF-N 252
            SFFNWW+     GI  +I  + Y Q+N++WA+GFGIPC+ MV    LFL G KTYR+  
Sbjct: 190 GSFFNWWFLSLSAGITLSIIVVVYVQDNVNWALGFGIPCLFMVMALALFLFGRKTYRYPR 249

Query: 253 TLKQSDKSPFFRIVRVFVASIRNLRAPASSITFQDE-QTAKDLSTSQQFKFLNKACIIVP 312
             ++   + F RI RVF+ + +N +     +T   + +         Q +FL KA  ++P
Sbjct: 250 GDREGKNNAFARIGRVFLVAFKNRKL---KLTHSGQLEVGSYKKCKGQLEFLAKA--LLP 309

Query: 313 IDSNQTAMTCSISELEEAKAILRILIIWVTVVVFTIAFSQDATFFTKQAATLDRSIMSGF 372
            +       CS  ++E+A A++R++ IW+T VV TI ++Q ATFFTKQ  T+DR I+ GF
Sbjct: 310 GEGG--VEPCSSRDVEDAMALVRLIPIWITSVVSTIPYAQYATFFTKQGVTVDRKILPGF 369

Query: 373 IIPAASLEALVSFTIVIFIVVYDLLFVPIAKKVTGNPSGITTLQRIGTGMVISTISMAVA 432
            IP AS +AL+  +I I +  Y+ +F+P+A+ +T  PSGIT LQRIG GMV+S+++M VA
Sbjct: 370 EIPPASFQALIGLSIFISVPTYERVFLPLARLITKKPSGITMLQRIGAGMVLSSLNMVVA 429

Query: 433 SLVEKKRLQTALEHGLIDIPEMTIPMRFWWLVPQYVLNGLADVFTVVGLQELCYNQVPKD 492
           +LVE KRL+TA EHGL+D P+ TIPM  WW VPQY+L G+ DVF++VG QE  Y+QVP +
Sbjct: 430 ALVEMKRLETAKEHGLVDRPDATIPMSIWWFVPQYLLLGMIDVFSLVGTQEFFYDQVPTE 489

Query: 493 LKSVGPAIFISILGMGNILSSLLISVIDTATKANGHRSWFSNNLNNAHLDYFYLLLAALS 552
           L+S+G A+ +S +G+ + LS  LI+VI+ AT  NG  SWF+ NLN AH+DYFY LLAA +
Sbjct: 490 LRSIGLALSLSAMGLASFLSGFLITVINWATGKNGGDSWFNTNLNRAHVDYFYWLLAAFT 549

Query: 553 VLGFVAFLFVAKSHVY 558
            +GF+AFL +++ +VY
Sbjct: 550 AIGFLAFLLLSRLYVY 558

BLAST of MELO3C022224.2 vs. Swiss-Prot
Match: sp|Q0WP01|PTR9_ARATH (Protein NRT1/ PTR FAMILY 5.10 OS=Arabidopsis thaliana OX=3702 GN=NPF5.10 PE=2 SV=1)

HSP 1 Score: 584.7 bits (1506), Expect = 1.1e-165
Identity = 304/567 (53.62%), Postives = 406/567 (71.60%), Query Frame = 0

Query: 1   MDLIDSMDHA-TPLLTDMVDGAVDYKGQPLLRSSSATWRSASFIIGMEVAERFAFYGIGA 60
           M +  ++D A TPLL       VDY+ +P ++SSS  WRSA FIIG+EVAERFA+YGI +
Sbjct: 1   MSISGAVDEAGTPLLA----VTVDYRNKPAVKSSSGGWRSAGFIIGVEVAERFAYYGISS 60

Query: 61  NLITYLTGPLRISVVAAAEIVNVWAGTSMLLTLFGAFLADSFFGRYRTI---------GL 120
           NLITYLTGPL  S  AAA  VN W+GT+ LL L GAF+ADSF GR+RTI         GL
Sbjct: 61  NLITYLTGPLGQSTAAAAANVNAWSGTASLLPLLGAFVADSFLGRFRTILAASALYIVGL 120

Query: 121 GLLVLSTLLPTASSSICAIPNKFAPCSSPKLHLILFFLSLCLIGIAQGGHRPCVQAFGAD 180
           G+L LS ++P    S C + N  + C SP+  +I FF +L L+ +AQGGH+PCVQAFGAD
Sbjct: 121 GVLTLSAMIP----SDCKVSNLLSSC-SPRFQVITFFSALYLVALAQGGHKPCVQAFGAD 180

Query: 181 QFDPQNPQEAKFKSSFFNWWYFGACFGIVAAIPTISYAQENLSWAVGFGIPCVSMVTGFI 240
           QFD + P+E K KSSFFNWWYFG CFG +  +  ++Y Q+NLSWA+GFGIPC++MV   +
Sbjct: 181 QFDEKEPEECKAKSSFFNWWYFGMCFGTLTTLWVLNYIQDNLSWALGFGIPCIAMVVALV 240

Query: 241 LFLLGTKTYRFNTLKQSDKSPFFRIVRVFVASIRNLRAPASSITFQDEQTA-KDLSTSQQ 300
           + LLGT TYRF ++++ D+SPF RI  V+VA+++N    A  +   +E+      S+SQQ
Sbjct: 241 VLLLGTCTYRF-SIRREDQSPFVRIGNVYVAAVKNWSVSALDVAAAEERLGLVSCSSSQQ 300

Query: 301 FKFLNKACIIVPIDSNQTAMTCSISELEEAKAILRILIIWVTVVVFTIAFSQDATFFTKQ 360
           F FLNKA +           +CSI ELEEAK++LR+  IW+T +V+ + F+Q  TFFTKQ
Sbjct: 301 FSFLNKALV-------AKNGSCSIDELEEAKSVLRLAPIWLTCLVYAVVFAQSPTFFTKQ 360

Query: 361 AATLDRSIMSGFIIPAASLEALVSFTIVIFIVVYDLLFVPIAKKVTGNPSGITTLQRIGT 420
            AT++RSI  G+ I  A+L++ +S +IVIFI +YD + +PIA+  T  P GIT LQRIGT
Sbjct: 361 GATMERSITPGYKISPATLQSFISLSIVIFIPIYDRVLIPIARSFTHKPGGITMLQRIGT 420

Query: 421 GMVISTISMAVASLVEKKRLQTALEHGLIDIPEMTIPMRFWWLVPQYVLNGLADVFTVVG 480
           G+ +S ++M VA+LVE KRL+TA ++GL+D P+ T+PM  WWLVPQYVL G+ DVF +VG
Sbjct: 421 GIFLSFLAMVVAALVEMKRLKTAADYGLVDSPDATVPMSVWWLVPQYVLFGITDVFAMVG 480

Query: 481 LQELCYNQVPKDLKSVGPAIFISILGMGNILSSLLISVIDTATKANGHRSWFSNNLNNAH 540
           LQE  Y+QVP +L+SVG A+++SI G+GN LSS +IS+I+ AT  +G  SWF+NNLN AH
Sbjct: 481 LQEFFYDQVPNELRSVGLALYLSIFGIGNFLSSFMISIIEKATSQSGQASWFANNLNQAH 540

Query: 541 LDYFYLLLAALSVLGFVAFLFVAKSHV 557
           LDYFY LLA LS +G  ++L+VAKS+V
Sbjct: 541 LDYFYWLLACLSFIGLASYLYVAKSYV 550

BLAST of MELO3C022224.2 vs. Swiss-Prot
Match: sp|Q0WSZ6|PTR23_ARATH (Protein NRT1/ PTR FAMILY 5.13 OS=Arabidopsis thaliana OX=3702 GN=NPF5.13 PE=2 SV=2)

HSP 1 Score: 540.8 bits (1392), Expect = 1.8e-152
Identity = 283/550 (51.45%), Postives = 386/550 (70.18%), Query Frame = 0

Query: 11  TPLLTDMVDGAVDYKGQPLLRSSSATWRSASFIIGMEVAERFAFYGIGANLITYLTGPLR 70
           T L  + V  AVD++G    RS +  WR+A FIIG+EVAERFA YGIG+NLI+YLTGPL 
Sbjct: 7   TSLQEEYVIDAVDHRGFSARRSITGRWRAAWFIIGVEVAERFANYGIGSNLISYLTGPLG 66

Query: 71  ISVVAAAEIVNVWAGTSMLLTLFGAFLADSFFGRYRTIGLGLLVLSTLLPTASSSICAIP 130
            S   AA  VN W+G S +L L GAF+AD+F GRY TI +   +    L   + S   IP
Sbjct: 67  QSTAVAAANVNAWSGISTILPLLGAFVADAFLGRYITIIIASFIYVLGLAFLTLSAFLIP 126

Query: 131 NKFAPCSSPKLHL-ILFFLSLCLIGIAQGGHRPCVQAFGADQFDPQNPQEAKFKSSFFNW 190
           N     SSP   L  LFF SL L+ I Q GH+PCVQAFGADQFD +NPQE   +SSFFNW
Sbjct: 127 NNTEVTSSPSSFLNALFFFSLYLVAIGQSGHKPCVQAFGADQFDEKNPQENSDRSSFFNW 186

Query: 191 WYFGACFGIVAAIPTISYAQENLSWAVGFGIPCVSMVTGFILFLLGTKTYRFNTLKQSDK 250
           WY   C GI  AI  + Y QEN+SWA+GFGIPCV MV   +LF+LG K+YRF+  +Q ++
Sbjct: 187 WYLSMCAGIGLAILVVVYIQENVSWALGFGIPCVFMVISLVLFVLGRKSYRFSKTRQEEE 246

Query: 251 -SPFFRIVRVFVASIRNLRAPASSI-TFQDEQTAKDLSTSQQFKFLNKACIIVPIDSNQT 310
            +PF RI RVF  + +N R  +S +   +  +  +   + ++  FLNKA ++VP DS++ 
Sbjct: 247 TNPFTRIGRVFFVAFKNQRLNSSDLCKVELIEANRSQESPEELSFLNKA-LLVPNDSDEG 306

Query: 311 AMTCSISELEEAKAILRILIIWVTVVVFTIAFSQDATFFTKQAATLDRSIMSGFIIPAAS 370
            + C   ++E+A A++R++ +W+T + + I F+Q  TFFTKQ  T++R+I  G  IP AS
Sbjct: 307 EVACKSRDVEDATALVRLIPVWLTTLAYAIPFAQYMTFFTKQGVTMERTIFPGVEIPPAS 366

Query: 371 LEALVSFTIVIFIVVYDLLFVPIAKKVTGNPSGITTLQRIGTGMVISTISMAVASLVEKK 430
           L+ L+S +IV+F+ +YD + VPI + +T +P GITTL+RIGTGMV++T++M VA+LVE K
Sbjct: 367 LQVLISISIVLFVPIYDRVLVPIGRSITKDPCGITTLKRIGTGMVLATLTMVVAALVESK 426

Query: 431 RLQTALEHGLIDIPEMTIPMRFWWLVPQYVLNGLADVFTVVGLQELCYNQVPKDLKSVGP 490
           RL+TA E+GLID P+ T+PM  WWL PQY+L GLADV T+VG+QE  Y+QVP +L+S+G 
Sbjct: 427 RLETAKEYGLIDQPKTTLPMSIWWLFPQYMLLGLADVHTLVGMQEFFYSQVPTELRSLGL 486

Query: 491 AIFISILGMGNILSSLLISVIDTATKANGHRSWFSNNLNNAHLDYFYLLLAALSVLGFVA 550
           AI++S +G+G++LSSLLI +ID AT  +   SWF++NLN AHLDYFY LLA +S +GF  
Sbjct: 487 AIYLSAMGVGSLLSSLLIYLIDLATGGDAGNSWFNSNLNRAHLDYFYWLLAVVSAVGFFT 546

Query: 551 FLFVAKSHVY 558
           FLF++KS++Y
Sbjct: 547 FLFISKSYIY 555

BLAST of MELO3C022224.2 vs. Swiss-Prot
Match: sp|Q8VZE2|PTR22_ARATH (Protein NRT1/ PTR FAMILY 5.14 OS=Arabidopsis thaliana OX=3702 GN=NPF5.14 PE=2 SV=2)

HSP 1 Score: 531.2 bits (1367), Expect = 1.4e-149
Identity = 279/548 (50.91%), Postives = 383/548 (69.89%), Query Frame = 0

Query: 13  LLTDMVDGAVDYKGQPLLRSSSATWRSASFIIGMEVAERFAFYGIGANLITYLTGPLRIS 72
           L  + V  AVD++G    RS++  WR+A FIIG+EVAERFA+YGIG+NLI+YLTGPL  S
Sbjct: 9   LQEEYVTDAVDHRGLAARRSNTGRWRAALFIIGVEVAERFAYYGIGSNLISYLTGPLGES 68

Query: 73  VVAAAEIVNVWAGTSMLLTLFGAFLADSFFGRYRTIGLGLLVLSTLLPTASSSICAIPNK 132
              AA  VN W+G + LL + GAF+AD+F GRYRTI +  L+    L   + S   IPN 
Sbjct: 69  TAVAAANVNAWSGIATLLPVLGAFVADAFLGRYRTIIISSLIYVLGLAFLTLSAFLIPNT 128

Query: 133 FAPCSSPKLHL-ILFFLSLCLIGIAQGGHRPCVQAFGADQFDPQNPQEAKFKSSFFNWWY 192
               SS    L +LFF SL L+ I Q GH+PCVQAFGADQFD ++ QE   +SSFFNWWY
Sbjct: 129 TEVTSSTSSFLNVLFFFSLYLVAIGQSGHKPCVQAFGADQFDEKDSQEKSDRSSFFNWWY 188

Query: 193 FGACFGIVAAIPTISYAQENLSWAVGFGIPCVSMVTGFILFLLGTKTYRFNTLKQSDK-S 252
                GI  AI  + Y QE  SWA GFGIPCV MV   +LF+ G + YR++  +  ++ +
Sbjct: 189 LSLSAGICFAILVVVYIQEEFSWAFGFGIPCVFMVISLVLFVSGRRIYRYSKRRHEEEIN 248

Query: 253 PFFRIVRVFVASIRNLRAPASSITFQDEQTAKDLSTS-QQFKFLNKACIIVPIDSNQTAM 312
           PF RI RVF  +++N R  +S +     +   + +TS ++  F NKA ++VP DS+Q   
Sbjct: 249 PFTRIGRVFFVALKNQRLSSSDLC----KVELEANTSPEKQSFFNKA-LLVPNDSSQGEN 308

Query: 313 TCSISELEEAKAILRILIIWVTVVVFTIAFSQDATFFTKQAATLDRSIMSGFIIPAASLE 372
               S++E+A A++R++ +W T + + I ++Q  TFFTKQ  T+DR+I+ G  IP ASL+
Sbjct: 309 ASKSSDVEDATALIRLIPVWFTTLAYAIPYAQYMTFFTKQGVTMDRTILPGVKIPPASLQ 368

Query: 373 ALVSFTIVIFIVVYDLLFVPIAKKVTGNPSGITTLQRIGTGMVISTISMAVASLVEKKRL 432
             +  +IV+F+ +YD +FVPIA+ +T  P GITTL+RIGTG+V+STI+M +A+LVE KRL
Sbjct: 369 VFIGISIVLFVPIYDRVFVPIARLITKEPCGITTLKRIGTGIVLSTITMVIAALVEFKRL 428

Query: 433 QTALEHGLIDIPEMTIPMRFWWLVPQYVLNGLADVFTVVGLQELCYNQVPKDLKSVGPAI 492
           +TA EHGLID PE T+PM  WWL+PQY+L GLADV+T+VG+QE  Y+QVP +L+S+G A+
Sbjct: 429 ETAKEHGLIDQPEATLPMSIWWLIPQYLLLGLADVYTLVGMQEFFYSQVPTELRSIGLAL 488

Query: 493 FISILGMGNILSSLLISVIDTATKANGHRSWFSNNLNNAHLDYFYLLLAALSVLGFVAFL 552
           ++S LG+G++LSSLLIS+ID AT  +   SWF++NLN AHLDYFY LLA +S +GF  FL
Sbjct: 489 YLSALGVGSLLSSLLISLIDLATGGDAGNSWFNSNLNRAHLDYFYWLLAIVSAVGFFTFL 548

Query: 553 FVAKSHVY 558
           F++KS++Y
Sbjct: 549 FISKSYIY 551

BLAST of MELO3C022224.2 vs. Swiss-Prot
Match: sp|Q9C7U1|PTR25_ARATH (Protein NRT1/ PTR FAMILY 5.12 OS=Arabidopsis thaliana OX=3702 GN=NPF5.12 PE=2 SV=1)

HSP 1 Score: 504.6 bits (1298), Expect = 1.4e-141
Identity = 276/557 (49.55%), Postives = 373/557 (66.97%), Query Frame = 0

Query: 18  VDGAVDYKGQPLLRSSSATWRSASFIIGMEVAERFAFYGIGANLITYLTGPLRISVVAAA 77
           V+ +VD++G P +RSSS  W+S+ F +  EVAE+FA++GI +NLITY T  L  S   AA
Sbjct: 19  VEFSVDFRGNPSIRSSSGAWKSSGFTMCAEVAEKFAYFGIASNLITYFTEALGESTAVAA 78

Query: 78  EIVNVWAGTSMLLTLFGAFLADSFFGRYRTI---------GLGLLVLSTLLPTASSSICA 137
             VN+W GT+  L L    +ADSF GR+RTI         GLGLL  S  +P    S+C 
Sbjct: 79  SNVNLWLGTAAFLPLIWGSIADSFLGRFRTILLTSSFYIMGLGLLTFSATIP----SLCN 138

Query: 138 IPNKFAPCSSPKLHLILFFLSLCLIGIAQGGHRPCVQAFGADQFDPQNPQEAKFKSSFFN 197
                  C S ++ +I+FF +L LI + +GG + C++AFGADQFD Q+P E+K KSS+FN
Sbjct: 139 DQETRESCVS-QVKVIIFFCALYLIALGEGGFKVCLRAFGADQFDEQDPNESKAKSSYFN 198

Query: 198 WWYFGACFGIVAAIPTISYAQENLSWAVGFGIPCVSMVTGFILFLLGTKTYRFNT---LK 257
           W YF    GI+      +Y QENLSWA+G+ IPC+SM+    LFLLG KTYRF+T    +
Sbjct: 199 WLYFAISIGILTTRLVTNYVQENLSWALGYAIPCLSMMLALFLFLLGIKTYRFSTGGEGR 258

Query: 258 QSDK--SPFFRIVRVFVASIRNLRAPASS--ITFQDEQTAKDLSTSQQFKFLNKACIIVP 317
           Q  K  +PF RI RVFVA+ RN R   S   +   +E T K       F+FL++A I   
Sbjct: 259 QGKKHDNPFVRIGRVFVAAARNRRQTPSDTCLLLPNESTKK-------FRFLDRAVI--- 318

Query: 318 IDSNQTAMTCSISELEEAKAILRILIIWVTVVVFTIAFSQDATFFTKQAATLDRSIMSGF 377
                   +C   E+EEAKA+L ++ IW+  +VF I F+Q  TFFTKQ +T+DRSI S  
Sbjct: 319 --------SCDSYEVEEAKAVLSLIPIWLCSLVFGIVFAQSPTFFTKQGSTMDRSISSTL 378

Query: 378 IIPAASLEALVSFTIVIFIVVYDLLFVPIAKKVTGNPSGITTLQRIGTGMVISTISMAVA 437
            +PAA+L+  +S  I++FI +YD LFVPIA+ +T  P+GITTLQRI TG+ +S ISM +A
Sbjct: 379 QVPAATLQCFISLAILVFIPIYDRLFVPIARSITRKPAGITTLQRISTGIFLSIISMVIA 438

Query: 438 SLVEKKRLQTALEHGLIDIPEMTIPMRFWWLVPQYVLNGLADVFTVVGLQELCYNQVPKD 497
           +LVE KRL+TA +HGL+D P+ T+PM   WL+PQY+L G++DVFT+VGLQE  Y +VP  
Sbjct: 439 ALVEMKRLKTARDHGLVDSPKATVPMSVCWLIPQYILFGVSDVFTMVGLQEFFYGEVPPQ 498

Query: 498 LKSVGPAIFISILGMGNILSSLLISVIDTATKANGHRSWFSNNLNNAHLDYFYLLLAALS 557
           L+S+G A+++SI+G+GN LSS ++SVI+ AT  +G  SWFSNNLN AHLDYFY LLA LS
Sbjct: 499 LRSMGLALYLSIIGIGNFLSSFMVSVIEEATSQSGQVSWFSNNLNQAHLDYFYWLLACLS 552

Query: 558 VLGFVAFLFVAKSHVYN 559
            L F+  ++ AKS++YN
Sbjct: 559 SLAFIFTVYFAKSYLYN 552

BLAST of MELO3C022224.2 vs. Swiss-Prot
Match: sp|Q9SK96|PTR10_ARATH (Protein NRT1/ PTR FAMILY 5.16 OS=Arabidopsis thaliana OX=3702 GN=NPF5.16 PE=2 SV=1)

HSP 1 Score: 496.5 bits (1277), Expect = 3.8e-139
Identity = 269/556 (48.38%), Postives = 372/556 (66.91%), Query Frame = 0

Query: 13  LLTDMVDGAVDYKGQPLLRSSSATWRSASFIIGMEVAERFAFYGIGANLITYLTGPLRIS 72
           L+ D V  +VD++G P  +SS+  WRSA +IIG+EV ERFA++GIG+NLITYLTGPL  S
Sbjct: 10  LIEDSVSDSVDHRGLPAGKSSTGGWRSAWYIIGVEVGERFAYFGIGSNLITYLTGPLGQS 69

Query: 73  VVAAAEIVNVWAGTSMLLTLFGAFLADSFFGRYRTI---------GLGLLVLSTLLPTAS 132
              AA  VN W+GT+ +L + GAF+AD++ GRYRTI         GLGLL LS++L    
Sbjct: 70  TATAAVNVNTWSGTASILPVLGAFIADAYLGRYRTIVVASLIYILGLGLLTLSSILILMG 129

Query: 133 SSICAIPNKFAPCSSPKLHLILFFLSLCLIGIAQGGHRPCVQAFGADQFDPQNPQEAKFK 192
            S     N+ A         ILFF SL L+ I QGGH+PCVQAFGADQFD  +P+E   +
Sbjct: 130 LSEQRQHNRNASAKPFFWVNILFFCSLYLVAIGQGGHKPCVQAFGADQFDVGDPKERISR 189

Query: 193 SSFFNWWYFGACFGIVAAIPTISYAQENLSWAVGFGIPCVSMVTGFILFLLGTKTYRF-N 252
            SFFNWW+     GI  +I  + Y Q+N++WA+GFGIPC+ MV    LFL G KTYR+  
Sbjct: 190 GSFFNWWFLSLSAGITLSIIVVVYVQDNVNWALGFGIPCLFMVMALALFLFGRKTYRYPR 249

Query: 253 TLKQSDKSPFFRIVRVFVASIRNLRAPASSITFQDE-QTAKDLSTSQQFKFLNKACIIVP 312
             ++   + F RI RVF+ + +N +     +T   + +         Q +FL KA  ++P
Sbjct: 250 GDREGKNNAFARIGRVFLVAFKNRKL---KLTHSGQLEVGSYKKCKGQLEFLAKA--LLP 309

Query: 313 IDSNQTAMTCSISELEEAKAILRILIIWVTVVVFTIAFSQDATFFTKQAATLDRSIMSGF 372
            +       CS  ++E+A A++R++ IW+T VV TI ++Q ATFFTKQ  T+DR I+ GF
Sbjct: 310 GEGG--VEPCSSRDVEDAMALVRLIPIWITSVVSTIPYAQYATFFTKQGVTVDRKILPGF 369

Query: 373 IIPAASLEALVSFTIVIFIVVYDLLFVPIAKKVTGNPSGITTLQRIGTGMVISTISMAVA 432
            IP AS +AL+  +I I +  Y+ +F+P+A+ +T  PSGIT LQRIG GMV+S+++M VA
Sbjct: 370 EIPPASFQALIGLSIFISVPTYERVFLPLARLITKKPSGITMLQRIGAGMVLSSLNMVVA 429

Query: 433 SLVEKKRLQTALEHGLIDIPEMTIPMRFWWLVPQYVLNGLADVFTVVGLQELCYNQVPKD 492
           +LVE KRL+TA EHGL+D P+ TIPM  WW VPQY+L G+ DVF++VG QE  Y+QVP +
Sbjct: 430 ALVEMKRLETAKEHGLVDRPDATIPMSIWWFVPQYLLLGMIDVFSLVGTQEFFYDQVPTE 489

Query: 493 LKSVGPAIFISILGMGNILSSLLISVIDTATKANGHRSWFSNNLNNAHLDYFYLLLAALS 552
           L+S+G A+ +S +G+ + LS  LI+VI+ AT  NG  SWF+ NLN AH+DYFY LLAA +
Sbjct: 490 LRSIGLALSLSAMGLASFLSGFLITVINWATGKNGGDSWFNTNLNRAHVDYFYWLLAAFT 549

Query: 553 VLGFVAFLFVAKSHVY 558
            +GF+AFL +++ +VY
Sbjct: 550 AIGFLAFLLLSRLYVY 558

BLAST of MELO3C022224.2 vs. TrEMBL
Match: tr|A0A1S4E2G7|A0A1S4E2G7_CUCME (protein NRT1/ PTR FAMILY 5.10-like OS=Cucumis melo OX=3656 GN=LOC103498766 PE=4 SV=1)

HSP 1 Score: 1072.8 bits (2773), Expect = 2.2e-310
Identity = 559/568 (98.42%), Postives = 559/568 (98.42%), Query Frame = 0

Query: 1   MDLIDSMDHATPLLTDMVDGAVDYKGQPLLRSSSATWRSASFIIGMEVAERFAFYGIGAN 60
           MDLIDSMDHATPLLTDMVDGAVDYKGQPLLRSSSATWRSASFIIGMEVAERFAFYGIGAN
Sbjct: 1   MDLIDSMDHATPLLTDMVDGAVDYKGQPLLRSSSATWRSASFIIGMEVAERFAFYGIGAN 60

Query: 61  LITYLTGPLRISVVAAAEIVNVWAGTSMLLTLFGAFLADSFFGRYRTI---------GLG 120
           LITYLTGPLRISVVAAAEIVNVWAGTSMLLTLFGAFLADSFFGRYRTI         GLG
Sbjct: 61  LITYLTGPLRISVVAAAEIVNVWAGTSMLLTLFGAFLADSFFGRYRTIVFASISYILGLG 120

Query: 121 LLVLSTLLPTASSSICAIPNKFAPCSSPKLHLILFFLSLCLIGIAQGGHRPCVQAFGADQ 180
           LLVLSTLLPTASSSICAIPNKFAPCSSPKLHLILFFLSLCLIGIAQGGHRPCVQAFGADQ
Sbjct: 121 LLVLSTLLPTASSSICAIPNKFAPCSSPKLHLILFFLSLCLIGIAQGGHRPCVQAFGADQ 180

Query: 181 FDPQNPQEAKFKSSFFNWWYFGACFGIVAAIPTISYAQENLSWAVGFGIPCVSMVTGFIL 240
           FDPQNPQEAKFKSSFFNWWYFGACFGIVAAIPTISYAQENLSWAVGFGIPCVSMVTGFIL
Sbjct: 181 FDPQNPQEAKFKSSFFNWWYFGACFGIVAAIPTISYAQENLSWAVGFGIPCVSMVTGFIL 240

Query: 241 FLLGTKTYRFNTLKQSDKSPFFRIVRVFVASIRNLRAPASSITFQDEQTAKDLSTSQQFK 300
           FLLGTKTYRFNTLKQSDKSPFFRIVRVFVASIRNLRAPASSITFQDEQTAKDLSTSQQFK
Sbjct: 241 FLLGTKTYRFNTLKQSDKSPFFRIVRVFVASIRNLRAPASSITFQDEQTAKDLSTSQQFK 300

Query: 301 FLNKACIIVPIDSNQTAMTCSISELEEAKAILRILIIWVTVVVFTIAFSQDATFFTKQAA 360
           FLNKACIIVPIDSNQTAMTCSISELEEAKAILRILIIWVTVVVFTIAFSQDATFFTKQAA
Sbjct: 301 FLNKACIIVPIDSNQTAMTCSISELEEAKAILRILIIWVTVVVFTIAFSQDATFFTKQAA 360

Query: 361 TLDRSIMSGFIIPAASLEALVSFTIVIFIVVYDLLFVPIAKKVTGNPSGITTLQRIGTGM 420
           TLDRSIMSGFIIPAASLEALVSFTIVIFIVVYDLLFVPIAKKVTGNPSGITTLQRIGTGM
Sbjct: 361 TLDRSIMSGFIIPAASLEALVSFTIVIFIVVYDLLFVPIAKKVTGNPSGITTLQRIGTGM 420

Query: 421 VISTISMAVASLVEKKRLQTALEHGLIDIPEMTIPMRFWWLVPQYVLNGLADVFTVVGLQ 480
           VISTISMAVASLVEKKRLQTALEHGLIDIPEMTIPMRFWWLVPQYVLNGLADVFTVVGLQ
Sbjct: 421 VISTISMAVASLVEKKRLQTALEHGLIDIPEMTIPMRFWWLVPQYVLNGLADVFTVVGLQ 480

Query: 481 ELCYNQVPKDLKSVGPAIFISILGMGNILSSLLISVIDTATKANGHRSWFSNNLNNAHLD 540
           ELCYNQVPKDLKSVGPAIFISILGMGNILSSLLISVIDTATKANGHRSWFSNNLNNAHLD
Sbjct: 481 ELCYNQVPKDLKSVGPAIFISILGMGNILSSLLISVIDTATKANGHRSWFSNNLNNAHLD 540

Query: 541 YFYLLLAALSVLGFVAFLFVAKSHVYNI 560
           YFYLLLAALSVLGFVAFLFVAKSHVYNI
Sbjct: 541 YFYLLLAALSVLGFVAFLFVAKSHVYNI 568

BLAST of MELO3C022224.2 vs. TrEMBL
Match: tr|A0A0A0KSZ8|A0A0A0KSZ8_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G652240 PE=4 SV=1)

HSP 1 Score: 1017.3 bits (2629), Expect = 1.3e-293
Identity = 533/584 (91.27%), Postives = 546/584 (93.49%), Query Frame = 0

Query: 1   MDLIDSMDHATPLLTDMVDGAVDYKGQPLLRSSSATWRSASFIIGMEVAERFAFYGIGAN 60
           MDLIDSMDHATPLLTDMVDGAVDYKGQPLLRSSSATWRSASFIIGMEVAERF+FYGIGAN
Sbjct: 1   MDLIDSMDHATPLLTDMVDGAVDYKGQPLLRSSSATWRSASFIIGMEVAERFSFYGIGAN 60

Query: 61  LITYLTGPLRISVVAAAEIVNVWAGTSMLLTLFGAFLADSFFGRYRTI---------GLG 120
           LITYLTGPL ISVVAAAEIVNVWA TSMLLTLFGAFLADSFFGRYRTI         GLG
Sbjct: 61  LITYLTGPLGISVVAAAEIVNVWAATSMLLTLFGAFLADSFFGRYRTIVFASISYILGLG 120

Query: 121 LLVLSTLLPTASSSICAIPNKFAPCSSPKLHLILFFLSLCLIGIAQGGHRPCVQAFGADQ 180
           LLVLSTLLPT SSSICAI NKF PCSSPKLHLILFFLSLCLIGIAQGGHRPCVQAFGADQ
Sbjct: 121 LLVLSTLLPTPSSSICAILNKFTPCSSPKLHLILFFLSLCLIGIAQGGHRPCVQAFGADQ 180

Query: 181 FDPQNPQEAKFKSSFFNWWYFGACFGIVAAIPTISYAQENLSWAVGFGIPCVSMVTGFIL 240
           FDPQNPQEAKFKSSFFNWWYFGACFGIVAAIPT+SYAQENLSWAVGFGIPCVSM+TGFIL
Sbjct: 181 FDPQNPQEAKFKSSFFNWWYFGACFGIVAAIPTVSYAQENLSWAVGFGIPCVSMLTGFIL 240

Query: 241 FLLGTKTYRFNTLKQSDKSPFFRIVRVFVASIRNLRAPASSITFQDEQTAKDLSTSQQFK 300
           FLLGT+TYRFNTLKQSDKSPF+RI RVF+ASIRNLRA AS+ITFQDEQTAK LSTSQQFK
Sbjct: 241 FLLGTETYRFNTLKQSDKSPFWRIGRVFLASIRNLRASASTITFQDEQTAKYLSTSQQFK 300

Query: 301 -----------------FLNKACIIVPIDSNQTAMTCSISELEEAKAILRILIIWVTVVV 360
                            FLNKACIIVPIDSNQ AMTCS+SELEEAKAILRILIIWVTVVV
Sbjct: 301 MHIMPTEDASFHVWCDRFLNKACIIVPIDSNQNAMTCSVSELEEAKAILRILIIWVTVVV 360

Query: 361 FTIAFSQDATFFTKQAATLDRSIMSGFIIPAASLEALVSFTIVIFIVVYDLLFVPIAKKV 420
           FTIAFSQDATFFTKQAATLDRSIMSGFIIPAASLEAL+SFTIVIFIVVYDLLFVPIAKKV
Sbjct: 361 FTIAFSQDATFFTKQAATLDRSIMSGFIIPAASLEALISFTIVIFIVVYDLLFVPIAKKV 420

Query: 421 TGNPSGITTLQRIGTGMVISTISMAVASLVEKKRLQTALEHGLIDIPEMTIPMRFWWLVP 480
           TGNPSGITTLQRIGTGMVISTISMAVASLVEKKRL+TAL+HGL+D PEMTIPMRFWWLVP
Sbjct: 421 TGNPSGITTLQRIGTGMVISTISMAVASLVEKKRLKTALDHGLVDTPEMTIPMRFWWLVP 480

Query: 481 QYVLNGLADVFTVVGLQELCYNQVPKDLKSVGPAIFISILGMGNILSSLLISVIDTATKA 540
           QYVLNGLADVFTVVGLQELCY+QVPKDLKSVGPAIFISILGMGNILSSLLISVIDTATKA
Sbjct: 481 QYVLNGLADVFTVVGLQELCYDQVPKDLKSVGPAIFISILGMGNILSSLLISVIDTATKA 540

Query: 541 NGHRSWFSNNLNNAHLDYFYLLLAALSVLGFVAFLFVAKSHVYN 559
           NGHRSWFSNNLN AHLDYFYLLLAALSV GFVAFLFVAKSHVYN
Sbjct: 541 NGHRSWFSNNLNKAHLDYFYLLLAALSVFGFVAFLFVAKSHVYN 584

BLAST of MELO3C022224.2 vs. TrEMBL
Match: tr|A0A1S3CAV1|A0A1S3CAV1_CUCME (protein NRT1/ PTR FAMILY 5.10-like OS=Cucumis melo OX=3656 GN=LOC103498725 PE=4 SV=1)

HSP 1 Score: 684.5 bits (1765), Expect = 2.0e-193
Identity = 360/563 (63.94%), Postives = 435/563 (77.26%), Query Frame = 0

Query: 7   MDHATPLLTDMVDGAVDYKGQPLLRSSSATWRSASFIIGMEVAERFAFYGIGANLITYLT 66
           M+  TPLL+  V+ AVD+KGQP LR  S  WRSASF+IG+EVAERFA+YGIG+NLITYLT
Sbjct: 1   MEQPTPLLSQTVESAVDFKGQPALRFCSGGWRSASFMIGVEVAERFAYYGIGSNLITYLT 60

Query: 67  GPLRISVVAAAEIVNVWAGTSMLLTLFGAFLADSFFGRYRTI---------GLGLLVLST 126
           GPL  SV  AAE VN+W+G SMLLTL GAFLADSFFGRYRTI         GL LL  S 
Sbjct: 61  GPLGQSVATAAETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLSLLSFSA 120

Query: 127 LLPTASSSICAIPNKFAPCSSPKLHLILFFLSLCLIGIAQGGHRPCVQAFGADQFDPQNP 186
           +LPT SS             + +  LI FF+SL LIGI QGGH+PCVQAFGADQFD  +P
Sbjct: 121 MLPTTSS------------QNSQFQLIFFFVSLYLIGIGQGGHKPCVQAFGADQFDALHP 180

Query: 187 QEAKFKSSFFNWWYFGACFGIVAAIPTISYAQENLSWAVGFGIPCVSMVTGFILFLLGTK 246
           QEAK KSSFFNWW+FG C G   AI  ++Y +ENLSW++GFGIPC+ MV    LFL GT 
Sbjct: 181 QEAKSKSSFFNWWFFGVCAGTFVAILLVTYTEENLSWSLGFGIPCIMMVIASFLFLFGTN 240

Query: 247 TYRFNTLKQSDKSPFFRIVRVFVASIRNLRAPASSITFQDEQTAKDLS--TSQQFKFLNK 306
           TYR+ ++K   K+PF RI RVFV++IRN RA +S++TF +E    DLS   + QF FLNK
Sbjct: 241 TYRY-SIKMYAKTPFLRIGRVFVSAIRNCRA-SSTVTFDEEGDGPDLSEQNTGQFSFLNK 300

Query: 307 ACIIVPIDSNQTAMTCSISELEEAKAILRILIIWVTVVVFTIAFSQDATFFTKQAATLDR 366
           AC IVP DSN+  + CS SE+EEAKA+LRI  +W+TV+VF I F+QD+TFFTKQ AT+DR
Sbjct: 301 AC-IVPKDSNKHGVMCSASEVEEAKAVLRIFPVWITVLVFAIVFAQDSTFFTKQGATIDR 360

Query: 367 SIMSGFIIPAASLEALVSFTIVIFIVVYDLLFVPIAKKVTGNPSGITTLQRIGTGMVIST 426
           SI+SGFIIPAA+L++ V  +IVIFI +YDLLFVPIA+  TG  SGITTLQRIGTG+V+S 
Sbjct: 361 SILSGFIIPAAALDSFVPLSIVIFITIYDLLFVPIARAFTGIQSGITTLQRIGTGLVVSA 420

Query: 427 ISMAVASLVEKKRLQTALEHGLIDIPEMTIPMRFWWLVPQYVLNGLADVFTVVGLQELCY 486
           ISM VA++VE+KRL+ A EHGL+D P++TIPM FWWLVPQY L GLA+VFT+VGLQE  Y
Sbjct: 421 ISMLVATMVERKRLRIADEHGLVDRPDITIPMSFWWLVPQYTLFGLAEVFTLVGLQEFFY 480

Query: 487 NQVPKDLKSVGPAIFISILGMGNILSSLLISVIDTATKANGHRSWFSNNLNNAHLDYFYL 546
           +QVP DLKS+G A + S+LGMG+ILSSLL+S+ID  T  +   SWFSNNLN AHLDYFYL
Sbjct: 481 DQVPTDLKSMGLAFYTSVLGMGSILSSLLVSIIDEVTGGSEQNSWFSNNLNKAHLDYFYL 540

Query: 547 LLAALSVLGFVAFLFVAKSHVYN 559
           LL+ALSV+ FVAFLFV+KSHV++
Sbjct: 541 LLSALSVVAFVAFLFVSKSHVHS 548

BLAST of MELO3C022224.2 vs. TrEMBL
Match: tr|A0A2I4FPR1|A0A2I4FPR1_9ROSI (protein NRT1/ PTR FAMILY 5.10-like OS=Juglans regia OX=51240 GN=LOC109000980 PE=4 SV=1)

HSP 1 Score: 635.6 bits (1638), Expect = 1.1e-178
Identity = 315/559 (56.35%), Postives = 423/559 (75.67%), Query Frame = 0

Query: 10  ATPLLTDMVDGAVDYKGQPLLRSSSATWRSASFIIGMEVAERFAFYGIGANLITYLTGPL 69
           ++PLL D VDGA D+KG+P LRS S TWRSASFIIG+E+AERFA+YGIG+NLI YLTGPL
Sbjct: 8   SSPLLLDTVDGAFDHKGRPALRSKSGTWRSASFIIGVEMAERFAYYGIGSNLIMYLTGPL 67

Query: 70  RISVVAAAEIVNVWAGTSMLLTLFGAFLADSFFGRYRTI---------GLGLLVLSTLLP 129
             S   AA+ VN W+GT+ LL L GAF+ADSF GRYRTI         GLGLL +S+++P
Sbjct: 68  GQSTATAAQNVNAWSGTASLLPLLGAFVADSFLGRYRTIVVASLIYILGLGLLTMSSIIP 127

Query: 130 TASSSICAIPNKFAPCSSPKLHLILFFLSLCLIGIAQGGHRPCVQAFGADQFDPQNPQEA 189
           + SS  C   +K  PCS P+  +ILFF SL L+ + QGGH+PCVQAFGADQF+ +NP+E 
Sbjct: 128 SLSSFGCEDASKSLPCSPPQPQVILFFFSLYLVALGQGGHKPCVQAFGADQFEGRNPKEC 187

Query: 190 KFKSSFFNWWYFGACFGIVAAIPTISYAQENLSWAVGFGIPCVSMVTGFILFLLGTKTYR 249
           K KSSFFNWWYFG C   +  +P ++  Q+N SW +GFGIPC +MV   ++F+LGT+TYR
Sbjct: 188 KAKSSFFNWWYFGLCACSLVTLPILNSIQDNFSWGLGFGIPCATMVVALVIFVLGTRTYR 247

Query: 250 FNTLKQSDKSPFFRIVRVFVASIRNLRAPASSITFQDE-QTAKDLSTSQQFKFLNKACII 309
           + + + ++KSPF R+ RVFVA+IRN R  +S+I  ++E +      +S++F+FLNKA ++
Sbjct: 248 Y-SFQGNEKSPFVRVGRVFVAAIRNWRTTSSAIAIEEETRGTLPHQSSEEFRFLNKA-LL 307

Query: 310 VPIDSNQTAMTCSISELEEAKAILRILIIWVTVVVFTIAFSQDATFFTKQAATLDRSIMS 369
            P  S +    CSI E+EEAKA+LR++ +W+T + + I F+Q +TFFTKQ AT++R ++ 
Sbjct: 308 APDGSKEDGKVCSIGEVEEAKAVLRLVPVWLTSLAYAIVFAQSSTFFTKQGATMNRRVVP 367

Query: 370 GFIIPAASLEALVSFTIVIFIVVYDLLFVPIAKKVTGNPSGITTLQRIGTGMVISTISMA 429
           GF +PAASL++ +S  IV+FI +YD +FVP+A+  TG PSGIT LQRIGTGMV+S ISMA
Sbjct: 368 GFDVPAASLQSFISLAIVLFIPIYDCIFVPMARAFTGKPSGITMLQRIGTGMVLSAISMA 427

Query: 430 VASLVEKKRLQTALEHGLIDIPEMTIPMRFWWLVPQYVLNGLADVFTVVGLQELCYNQVP 489
           +A++VE  RL+TA E+GL+D+P +T+PM  WWL PQYVL G+ADVFT+VGLQE  Y+QVP
Sbjct: 428 IAAVVEMVRLKTAQEYGLVDLPGVTVPMSVWWLAPQYVLFGIADVFTMVGLQEFFYDQVP 487

Query: 490 KDLKSVGPAIFISILGMGNILSSLLISVIDTATKANGHRSWFSNNLNNAHLDYFYLLLAA 549
            + +SVG A+++SI G+G+ LSS L+SVI +AT  +G  SWF+NNLN AHLDYFY LL  
Sbjct: 488 SEFRSVGLALYLSIFGVGSFLSSFLVSVIVSATGGDGQDSWFANNLNRAHLDYFYCLLCG 547

Query: 550 LSVLGFVAFLFVAKSHVYN 559
           LSV+   AFL++AKS++YN
Sbjct: 548 LSVIELAAFLYIAKSYIYN 564

BLAST of MELO3C022224.2 vs. TrEMBL
Match: tr|A0A2C9U3A5|A0A2C9U3A5_MANES (Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_18G105300 PE=4 SV=1)

HSP 1 Score: 631.7 bits (1628), Expect = 1.6e-177
Identity = 320/564 (56.74%), Postives = 419/564 (74.29%), Query Frame = 0

Query: 5   DSMDHATPLLTDMVDGAVDYKGQPLLRSSSATWRSASFIIGMEVAERFAFYGIGANLITY 64
           DS D   PLL D V+G+VDYKG+ + + +S  WRSASFIIG+EVAERFA+YGI +NLIT+
Sbjct: 10  DSPDIRAPLLYDTVEGSVDYKGRSVYKFNSGGWRSASFIIGVEVAERFAYYGIASNLITF 69

Query: 65  LTGPLRISVVAAAEIVNVWAGTSMLLTLFGAFLADSFFGRYRTI---------GLGLLVL 124
           LTGPL  S   AAE VN W+GT+ LL L GAF+ADSF GR+RTI         GLGLL L
Sbjct: 70  LTGPLGQSTATAAENVNAWSGTASLLPLLGAFVADSFLGRFRTIIVASLIYILGLGLLTL 129

Query: 125 STLLPTASSSICAIPNKFAPCSSPKLHLILFFLSLCLIGIAQGGHRPCVQAFGADQFDPQ 184
           S +LP+ S+S C   N    CS+  + ++LFF SL L+ + QGGH+PCVQAFGADQFD Q
Sbjct: 130 SAILPSFSASDCPSTNISTKCSANPIQVVLFFFSLYLVALGQGGHKPCVQAFGADQFDGQ 189

Query: 185 NPQEAKFKSSFFNWWYFGACFGIVAAIPTISYAQENLSWAVGFGIPCVSMVTGFILFLLG 244
           +P+E K KSSFFNWWYF  C G +  +  ++Y Q+NL+WA+GFGIPC+ MV   ILFLLG
Sbjct: 190 DPKEYKAKSSFFNWWYFCMCSGTLVTLLFLNYIQDNLNWALGFGIPCIMMVIALILFLLG 249

Query: 245 TKTYRFNTLKQSDKSPFFRIVRVFVASIRNLRAPASSITFQDEQTAK-DLSTSQQFKFLN 304
           TKTYR+ ++K  +KS F RI  VFV++I+N R   S++  ++E        +S+QFKFLN
Sbjct: 250 TKTYRY-SVKVEEKSAFLRIGLVFVSAIKNWRTSPSALALEEETRGMLPHQSSEQFKFLN 309

Query: 305 KACIIVPIDSNQTAMTCSISELEEAKAILRILIIWVTVVVFTIAFSQDATFFTKQAATLD 364
           KA ++ P  S +T   CS+SE+EEAKA+LR++ IW T +V+ +AF+Q +TFFTKQ  T+D
Sbjct: 310 KA-LLTPNGSKETGKVCSLSEIEEAKAVLRLVPIWATCLVYAVAFAQSSTFFTKQGVTMD 369

Query: 365 RSIMSGFIIPAASLEALVSFTIVIFIVVYDLLFVPIAKKVTGNPSGITTLQRIGTGMVIS 424
           RSI  GF +P A+L++ +S  IV+FI +YD +FVPIA+ +TG PSGIT LQRIGTGM +S
Sbjct: 370 RSISPGFKVPPATLQSFISLAIVLFIPIYDRIFVPIARALTGKPSGITMLQRIGTGMFLS 429

Query: 425 TISMAVASLVEKKRLQTALEHGLIDIPEMTIPMRFWWLVPQYVLNGLADVFTVVGLQELC 484
            +SMA+A+L+E KRL+ A EHGLID P +T+PM  WWLVPQYVL GLADVFT+VGLQE  
Sbjct: 430 ALSMAIAALIETKRLEVAREHGLIDKPNVTVPMSIWWLVPQYVLFGLADVFTMVGLQEFF 489

Query: 485 YNQVPKDLKSVGPAIFISILGMGNILSSLLISVIDTATKANGHRSWFSNNLNNAHLDYFY 544
           Y+QVP +L+SVG ++++SI G+G+ +SS L+S I+ AT   GH SWF+NNLN  HL+YFY
Sbjct: 490 YDQVPSELRSVGLSLYLSIFGVGSFISSFLVSAIEKATGGEGHDSWFANNLNRGHLNYFY 549

Query: 545 LLLAALSVLGFVAFLFVAKSHVYN 559
            LLAALSV+    +L+ AK++VYN
Sbjct: 550 WLLAALSVVQLFVYLYFAKNYVYN 571