MELO3C021067 (gene) Melon (DHL92) v3.5.1

BLAST of MELO3C021067 vs. Swiss-Prot
Match: PMEU1_SOLLC (Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum GN=PMEU1 PE=2 SV=1)

HSP 1 Score: 565.1 bits (1455), Expect = 9.1e-160
Identity = 302/597 (50.59%), Postives = 405/597 (67.84%), Query Frame = 1

Query: 5   KLSLALFATLLLTNAII-----VRANSNEN-------GVMDNISSLIETSCAITLYPQLC 64
           K+ LA+ A++LL  A+I     V+++S  +        +  +  ++++++C+ TL+P+LC
Sbjct: 20  KIYLAIVASVLLVAAVIGVVAGVKSHSKNSDDHADIMAISSSAHAIVKSACSNTLHPELC 79

Query: 65  HSTISSIIGTSN-LLSLKDIVQLSLSVAMDAAKLNNGNIKKNLTMSFNNVSKRDRIPLHD 124
           +S I ++   S  + S KD+++LSL++ + A + N   +K+ L  +   ++ R+++ LHD
Sbjct: 80  YSAIVNVSDFSKKVTSQKDVIELSLNITVKAVRRNYYAVKE-LIKTRKGLTPREKVALHD 139

Query: 125 CVKSTDRTIYELDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEK 184
           C+++ D T+ EL  A+ED   YPNKKSL  + +DLKT +SSAITNQ TCLDGF  D+ +K
Sbjct: 140 CLETMDETLDELHTAVEDLELYPNKKSLKEHVEDLKTLISSAITNQETCLDGFSHDEADK 199

Query: 185 GVLRVIENTHIHATKLCSNALALVKKLT-TEIALIDEKNLVVHDFPYKITSISSQINDLK 244
            V +V+     H  K+CSNALA++  +T T+IA                       N++K
Sbjct: 200 KVRKVLLKGQKHVEKMCSNALAMICNMTDTDIA-----------------------NEMK 259

Query: 245 IVLFSNQEEGENGRREDLENGIKWPEWMSIQDQKLLESSSSEAAADVVVAVDGSGNYKTV 304
           +   +N        R+ +E+  +WPEW+S  D++LL+SS+     DVVVA DGSG+YKTV
Sbjct: 260 LSAPANN-------RKLVEDNGEWPEWLSAGDRRLLQSST--VTPDVVVAADGSGDYKTV 319

Query: 305 SEAVEAAPSKNSKRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWS 364
           SEAV  AP K+SKRY+I+IKAGVYRENVDVP  K NIMF GDG+SNTIITA R+   G +
Sbjct: 320 SEAVRKAPEKSSKRYVIRIKAGVYRENVDVPKKKTNIMFMGDGKSNTIITASRNVQDGST 379

Query: 365 TFNSATVVAVGDGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHS 424
           TF+SATVV V    LARDI+F+NTAG +  QAVAL VGSD SAFYRC ML YQDTLYVHS
Sbjct: 380 TFHSATVVRVAGKVLARDITFQNTAGASKHQAVALCVGSDLSAFYRCDMLAYQDTLYVHS 439

Query: 425 NRQFFVNCVVAGTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQ 484
           NRQFFV C+VAGTVDF+FGN AAV Q+ D+  R+PG  Q+NMVTAQ RTD NQNTGIVIQ
Sbjct: 440 NRQFFVQCLVAGTVDFIFGNGAAVFQDCDIHARRPGSGQKNMVTAQGRTDPNQNTGIVIQ 499

Query: 485 KCRIKATSDLEPVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGD--IKKPY 544
           KCRI ATSDL PV K FP++LGRPW+EY+R V+MQ++IT+VI   GW  WNG+  +   +
Sbjct: 500 KCRIGATSDLRPVQKSFPTYLGRPWKEYSRTVIMQSSITDVIQPAGWHEWNGNFALDTLF 559

Query: 545 FAEYDNNGAGANTSGRVPW---SLVINEADAKTFTAERFIGGADWLPSTGFPYQLGL 583
           + EY N GAGA TSGRV W    ++ +  +A+ +T  RFI G  WL STGFP+ LGL
Sbjct: 560 YGEYANTGAGAPTSGRVKWKGHKVITSSTEAQAYTPGRFIAGGSWLSSTGFPFSLGL 583

BLAST of MELO3C021067 vs. Swiss-Prot
Match: PME3_CITSI (Pectinesterase 3 OS=Citrus sinensis PE=1 SV=1)

HSP 1 Score: 564.3 bits (1453), Expect = 1.5e-159
Identity = 300/591 (50.76%), Postives = 399/591 (67.51%), Query Frame = 1

Query: 5   KLSLALFATLLLTNAII-------VRANSNENGVMDNISSLIETSCAITLYPQLCHSTIS 64
           KL LALFATLL+  A+I        R NS +NG   +  +++++SC+ T YP LC S I+
Sbjct: 29  KLFLALFATLLVVAAVIGIVAGVNSRKNSGDNGNEPH-HAILKSSCSSTRYPDLCFSAIA 88

Query: 65  SIIGTSN-LLSLKDIVQLSLSVAMDAAKLNNGNIKKNLTMSFNNVSKRDRIPLHDCVKST 124
           ++   S  + S KD++++SL++   A + N   I+K L  +  N++KR+++ LHDC+++ 
Sbjct: 89  AVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRT--NLTKREKVALHDCLETI 148

Query: 125 DRTIYELDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKGVLRV 184
           D T+ EL KA+ED  EYPNKKSL+ +ADDLKT +S+A+TNQ TCLDGF  D   K V   
Sbjct: 149 DETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGTCLDGFSHDDANKHVRDA 208

Query: 185 IENTHIHATKLCSNALALVKKLTTEIALIDEKNLVVHDFPYKITSISSQINDLKIVLFSN 244
           + +  +H  K+CSNALA++K +T    +I   +                 N+ K++  ++
Sbjct: 209 LSDGQVHVEKMCSNALAMIKNMTDTDMMIMRTS-----------------NNRKLIEETS 268

Query: 245 QEEGENGRREDLENGIKWPEWMSIQDQKLLESSSSEAAADVVVAVDGSGNYKTVSEAVEA 304
             +G             WP W+S  D++LL+SSS     +VVVA DGSGN+KTV+ +V A
Sbjct: 269 TVDG-------------WPAWLSTGDRRLLQSSS--VTPNVVVAADGSGNFKTVAASVAA 328

Query: 305 APSKNSKRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSAT 364
           AP   +KRYII+IKAGVYRENV+V    +NIMF GDGR+ TIIT  R+   G +TF SAT
Sbjct: 329 APQGGTKRYIIRIKAGVYRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSAT 388

Query: 365 VVAVGDGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFV 424
           V  VG+GFLARDI+F+NTAGP+  QAVALRVG+D SAFY C ML YQDTLYVHSNRQFFV
Sbjct: 389 VAVVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFV 448

Query: 425 NCVVAGTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKA 484
           NC++AGTVDF+FGNAAAV+QN D+  RKP   Q+NMVTAQ R D NQNTGIVIQK RI A
Sbjct: 449 NCLIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRADPNQNTGIVIQKSRIGA 508

Query: 485 TSDLEPVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGD--IKKPYFAEYDN 544
           TSDL+PV   FP++LGRPW+EY+R V+MQ++IT+VI   GW  W+G+  +   ++ E+ N
Sbjct: 509 TSDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQN 568

Query: 545 NGAGANTSGRVPWS---LVINEADAKTFTAERFIGGADWLPSTGFPYQLGL 583
            GAGA TSGRV W    ++ +  +A+ FT   FI G+ WL STGFP+ LGL
Sbjct: 569 AGAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

BLAST of MELO3C021067 vs. Swiss-Prot
Match: PME1_CITSI (Pectinesterase 1 OS=Citrus sinensis GN=PECS-1.1 PE=2 SV=1)

HSP 1 Score: 563.5 bits (1451), Expect = 2.6e-159
Identity = 300/591 (50.76%), Postives = 396/591 (67.01%), Query Frame = 1

Query: 5   KLSLALFATLLLTNAII-------VRANSNENGVMDNISSLIETSCAITLYPQLCHSTIS 64
           KL LALFATLL+  A+I        R NS +NG   +  +++++SC+ T YP LC S I+
Sbjct: 29  KLFLALFATLLVVAAVIGIVAGVNSRKNSGDNGNEPH-HAILKSSCSSTRYPDLCFSAIA 88

Query: 65  SIIGTSN-LLSLKDIVQLSLSVAMDAAKLNNGNIKKNLTMSFNNVSKRDRIPLHDCVKST 124
           ++   S  + S KD++++SL++   A + N   I+K L  +  N++KR+++ LHDC+++ 
Sbjct: 89  AVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRT--NLTKREKVALHDCLETI 148

Query: 125 DRTIYELDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKGVLRV 184
           D T+ EL KA+ED  EYPNKKSL+ +ADDLKT +S+A+TNQ TCLDGF  D   K V   
Sbjct: 149 DETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGTCLDGFSHDDANKHVRDA 208

Query: 185 IENTHIHATKLCSNALALVKKLTTEIALIDEKNLVVHDFPYKITSISSQINDLKIVLFSN 244
           + +  +H  K+CSNALA++K +T                            D+ I+  SN
Sbjct: 209 LSDGQVHVEKMCSNALAMIKNMTD--------------------------TDMMIMRTSN 268

Query: 245 QEEGENGRREDLENGIKWPEWMSIQDQKLLESSSSEAAADVVVAVDGSGNYKTVSEAVEA 304
             +      E+      WP W+S  D++LL+SSS     + VVA DGSGN+KTV+ AV A
Sbjct: 269 NRK----LTEETSTVDGWPAWLSPGDRRLLQSSS--VTPNAVVAADGSGNFKTVAAAVAA 328

Query: 305 APSKNSKRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSAT 364
           AP   +KRYII+IKAGVYRENV+V    +NIMF GDGR+ TIIT  R+   G +TF SAT
Sbjct: 329 APQGGTKRYIIRIKAGVYRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSAT 388

Query: 365 VVAVGDGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFV 424
              VG+GFLARDI+F+NTAGP+  QAVALRVG+D SAFY C ML YQDTLYVHSNRQFFV
Sbjct: 389 AAVVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFV 448

Query: 425 NCVVAGTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKA 484
           NC++AGTVDF+FGNAAAV+QN D+  RKP   Q+NMVTAQ RTD NQNTGIVIQK RI A
Sbjct: 449 NCLIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGA 508

Query: 485 TSDLEPVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGD--IKKPYFAEYDN 544
           TSDL+PV   FP++LGRPW+EY+R V+MQ++IT++I   GW  W+G+  +   ++ E+ N
Sbjct: 509 TSDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQN 568

Query: 545 NGAGANTSGRVPWS---LVINEADAKTFTAERFIGGADWLPSTGFPYQLGL 583
           +GAGA TSGRV W    ++ +  +A+ FT   FI G+ WL STGFP+ LGL
Sbjct: 569 SGAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

BLAST of MELO3C021067 vs. Swiss-Prot
Match: PME3_ARATH (Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana GN=PME3 PE=2 SV=2)

HSP 1 Score: 531.2 bits (1367), Expect = 1.5e-149
Identity = 293/597 (49.08%), Postives = 389/597 (65.16%), Query Frame = 1

Query: 2   ENTKLSLALFATLLLTNAIIV-----RANSNENGVMDNIS-SLIETSCAITLYPQLCHST 61
           +N KL L   A  LL  A +       + +NE   +   S +++ +SC+ T YP+LC S 
Sbjct: 16  KNKKLVLLSAAVALLFVAAVAGISAGASKANEKRTLSPSSHAVLRSSCSSTRYPELCISA 75

Query: 62  ISSIIGTSNLLSLKDIVQLSLSVAMDAAKLNNGNIKKNLTMSFNNVSKRDRIPLHDCVKS 121
           + +  G   L S KD+++ S+++ + A + N   +KK L      ++ R++  LHDC+++
Sbjct: 76  VVTAGGVE-LTSQKDVIEASVNLTITAVEHNYFTVKK-LIKKRKGLTPREKTALHDCLET 135

Query: 122 TDRTIYELDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKGVLR 181
            D T+ EL + +ED   YP KK+L  +A DLKT +SSAITNQ TCLDGF  D  +K V +
Sbjct: 136 IDETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSHDDADKQVRK 195

Query: 182 VIENTHIHATKLCSNALALVKKLT-TEIALIDEKNLVVHDFPYKITSISSQINDLKIVLF 241
            +    IH   +CSNALA++K +T T+IA  ++K         KITS + ++ +      
Sbjct: 196 ALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKA--------KITSNNRKLKE------ 255

Query: 242 SNQEEGEN----GRREDLENGIKWPEWMSIQDQKLLESSSSEAAADVVVAVDGSGNYKTV 301
            NQE        G  E    G  WP W+S  D++LL+ S  +A  D  VA DGSG +KTV
Sbjct: 256 ENQETTVAVDIAGAGELDSEG--WPTWLSAGDRRLLQGSGVKA--DATVAADGSGTFKTV 315

Query: 302 SEAVEAAPSKNSKRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWS 361
           + AV AAP  ++KRY+I IKAGVYRENV+V   K+NIMF GDGR+ TIIT  R+   G +
Sbjct: 316 AAAVAAAPENSNKRYVIHIKAGVYRENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGST 375

Query: 362 TFNSATVVAVGDGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHS 421
           TF+SATV AVG+ FLARDI+F+NTAGP+  QAVALRVGSD SAFY C ML YQDTLYVHS
Sbjct: 376 TFHSATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHS 435

Query: 422 NRQFFVNCVVAGTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQ 481
           NRQFFV C++AGTVDF+FGNAA V+Q+ D+  R+P   Q+NMVTAQ RTD NQNTGIVIQ
Sbjct: 436 NRQFFVKCLIAGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQ 495

Query: 482 KCRIKATSDLEPVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGD--IKKPY 541
           KCRI ATSDL+ V   FP++LGRPW+EY++ V+MQ+ I++VI  +GWS W G   +    
Sbjct: 496 KCRIGATSDLQSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLT 555

Query: 542 FAEYDNNGAGANTSGRVPW---SLVINEADAKTFTAERFIGGADWLPSTGFPYQLGL 583
           + EY N GAGA T+ RV W    ++   A+A+ +TA +FIGG  WL STGFP+ LGL
Sbjct: 556 YREYSNTGAGAGTANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592

BLAST of MELO3C021067 vs. Swiss-Prot
Match: PME2_ARATH (Pectinesterase 2 OS=Arabidopsis thaliana GN=PME2 PE=2 SV=2)

HSP 1 Score: 522.3 bits (1344), Expect = 6.7e-147
Identity = 285/590 (48.31%), Postives = 386/590 (65.42%), Query Frame = 1

Query: 6   LSLALFATLLLTNAIIVRA---NSNENGVMDNISS----LIETSCAITLYPQLCHSTISS 65
           LS A  A LLL + + + A   N N+N  +  +SS    ++++ C+ TLYP+LC S +++
Sbjct: 22  LSSAAIALLLLASIVGIAATTTNQNKNQKITTLSSTSHAILKSVCSSTLYPELCFSAVAA 81

Query: 66  IIGTSNLLSLKDIVQLSLSVAMDAAKLNNGNIKKNLTMSFNNVSKRDRIPLHDCVKSTDR 125
             G   L S K++++ SL++   A K N   +KK L      ++ R+   LHDC+++ D 
Sbjct: 82  T-GGKELTSQKEVIEASLNLTTKAVKHNYFAVKK-LIAKRKGLTPREVTALHDCLETIDE 141

Query: 126 TIYELDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKGVLRVIE 185
           T+ EL  A+ED  +YP +KSL  +ADDLKT +SSAITNQ TCLDGF  D  ++ V + + 
Sbjct: 142 TLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQGTCLDGFSYDDADRKVRKALL 201

Query: 186 NTHIHATKLCSNALALVKKLT-TEIALIDEKNLVVHDFPYKITSISSQINDLKIVLFSNQ 245
              +H   +CSNALA++K +T T+IA     N  + D     T+ +++   LK V     
Sbjct: 202 KGQVHVEHMCSNALAMIKNMTETDIA-----NFELRDKSSTFTNNNNR--KLKEVTGDLD 261

Query: 246 EEGENGRREDLENGIKWPEWMSIQDQKLLESSSSEAAADVVVAVDGSGNYKTVSEAVEAA 305
            +G             WP+W+S+ D++LL+ S+ +A  D  VA DGSG++ TV+ AV AA
Sbjct: 262 SDG-------------WPKWLSVGDRRLLQGSTIKA--DATVADDGSGDFTTVAAAVAAA 321

Query: 306 PSKNSKRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATV 365
           P K++KR++I IKAGVYRENV+V   K NIMF GDGR  TIIT  R+   G +TF+SATV
Sbjct: 322 PEKSNKRFVIHIKAGVYRENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHSATV 381

Query: 366 VAVGDGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVN 425
            AVG+ FLARDI+F+NTAGP+  QAVALRVGSD SAFY+C M  YQDTLYVHSNRQFFV 
Sbjct: 382 AAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQFFVK 441

Query: 426 CVVAGTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKAT 485
           C + GTVDF+FGNAAAV+Q+ D+  R+P   Q+NMVTAQ R+D NQNTGIVIQ CRI  T
Sbjct: 442 CHITGTVDFIFGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCRIGGT 501

Query: 486 SDLEPVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGD--IKKPYFAEYDNN 545
           SDL  V   FP++LGRPW+EY+R V+MQ+ I++VI  +GW  W+G   +    + EY N 
Sbjct: 502 SDLLAVKGTFPTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYREYLNR 561

Query: 546 GAGANTSGRVPW---SLVINEADAKTFTAERFIGGADWLPSTGFPYQLGL 583
           G GA T+ RV W    ++ ++ +A+ FTA +FIGG  WL STGFP+ L L
Sbjct: 562 GGGAGTANRVKWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPFSLSL 587

BLAST of MELO3C021067 vs. TrEMBL
Match: A0A0A0K6S6_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_7G343850 PE=4 SV=1)

HSP 1 Score: 1034.2 bits (2673), Expect = 5.9e-299
Identity = 526/584 (90.07%), Postives = 550/584 (94.18%), Query Frame = 1

Query: 1   MENTKLSLALFATLLLTNAIIVRANSNENGVMDNISSLIETSCAITLYPQLCHSTISSII 60
           MENTKLSLALFATLLLTNAIIVRANSNENGVM+NI S+IETSCAITLYPQLCHSTISSI+
Sbjct: 1   MENTKLSLALFATLLLTNAIIVRANSNENGVMNNILSVIETSCAITLYPQLCHSTISSIL 60

Query: 61  GTSNLLSLKDIVQLSLSVAMDAAKLNNGNIKKNLTMSFNNVSKRDRIPLHDCVKSTDRTI 120
           GTSNLLSLKDIV+LSLSVAMDAAKLNN NIKK L+MS N V KRDRI LHDCVK+TDRTI
Sbjct: 61  GTSNLLSLKDIVELSLSVAMDAAKLNNENIKK-LSMSGNIVGKRDRIALHDCVKTTDRTI 120

Query: 121 YELDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKGVLRVIENT 180
           YELDKAI+DF EYPNKKSL SYADDLKTFLSSAITNQVTCLDG   DKTEK VLR+IENT
Sbjct: 121 YELDKAIQDFSEYPNKKSLASYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLRLIENT 180

Query: 181 HIHATKLCSNALALVKKLTTEIALIDEKNLVVHDFPYKITSISSQINDLKIVLFSNQEEG 240
           H   TKLCSNALALVKKLTT++AL DEK+LVVHDFPYKITSISSQI+D KIVLFSNQE+G
Sbjct: 181 HNQVTKLCSNALALVKKLTTDVALTDEKSLVVHDFPYKITSISSQIDDPKIVLFSNQEDG 240

Query: 241 ENGRREDLENGIKWPEWMSIQDQKLLESSSSEAAADVVVAVDGSGNYKTVSEAVEAAPSK 300
           E+ RREDLENGIKWPEWMSIQDQ LL+SSS EAA DVVVA DG+GNYKTVSEAV+AAPSK
Sbjct: 241 ESRRREDLENGIKWPEWMSIQDQMLLDSSSEEAA-DVVVAADGTGNYKTVSEAVKAAPSK 300

Query: 301 NSKRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATVVAV 360
           NS RYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITA RSHGSGWSTFNSATVVAV
Sbjct: 301 NS-RYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITADRSHGSGWSTFNSATVVAV 360

Query: 361 GDGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVNCVV 420
           GDGFLARDI+F+NTAG ANGQAVALRVGSDHSAFYRC ML YQDTLYVHSNRQFFV CVV
Sbjct: 361 GDGFLARDITFQNTAGSANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFFVKCVV 420

Query: 421 AGTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKATSDL 480
           AGTVDF+FGNAAAVIQNSDLTPRKPGPNQ NMVTAQSRTDLNQNTGIVIQKCRIKATSDL
Sbjct: 421 AGTVDFIFGNAAAVIQNSDLTPRKPGPNQNNMVTAQSRTDLNQNTGIVIQKCRIKATSDL 480

Query: 481 EPVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGDIKKPYFAEYDNNGAGAN 540
           EPVIKEFPSFLGRPWEEYARVVVMQT+ITNVIDK+GWSTWNGDIKKPYFAEYDNNGAGA+
Sbjct: 481 EPVIKEFPSFLGRPWEEYARVVVMQTSITNVIDKEGWSTWNGDIKKPYFAEYDNNGAGAD 540

Query: 541 TSGRVPWSLVINEADAKTFTAERFIGGADWLPSTGFPYQLGLYN 585
           TSGRV WSLVINEA+AKTFTAE FI GA WLPSTGFPYQLGLYN
Sbjct: 541 TSGRVSWSLVINEAEAKTFTAEPFIDGAGWLPSTGFPYQLGLYN 581

BLAST of MELO3C021067 vs. TrEMBL
Match: A0A0A0K9Y3_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_7G343870 PE=4 SV=1)

HSP 1 Score: 964.9 bits (2493), Expect = 4.4e-278
Identity = 488/583 (83.70%), Postives = 527/583 (90.39%), Query Frame = 1

Query: 1   MENTKLSLALFATLLLTNAIIVRANSNENGVMDNISSLIETSCAITLYPQLCHSTISSII 60
           MEN KLSLAL A LLLTNAI+VRANSNEN       S+IETSCAITLYPQLCHSTISSI+
Sbjct: 1   MENNKLSLALLAILLLTNAIVVRANSNEN-------SVIETSCAITLYPQLCHSTISSIV 60

Query: 61  GTSNLLSLKDIVQLSLSVAMDAAKLNNGNIKKNLTMSFNNVSKRDRIPLHDCVKSTDRTI 120
           GTSNLLSLKDI ++SLSVAMDAAK NN NIKK L +S NNVSKRD+I LHDCV++TDRTI
Sbjct: 61  GTSNLLSLKDIFEVSLSVAMDAAKHNNKNIKK-LMVSTNNVSKRDKIGLHDCVETTDRTI 120

Query: 121 YELDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKGVLRVIENT 180
           YEL KAIE FREYPNK+SLT YADDLKTFLSSAITNQVTCLDG   DKTEK VLR+IEN 
Sbjct: 121 YELGKAIEVFREYPNKRSLTLYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLRLIENA 180

Query: 181 HIHATKLCSNALALVKKLTTEIALIDEKNLVVHDFPYKITSISSQINDLKIVLFSNQEEG 240
           HIH TKLCSNALALV+KLTT+IA+ DEK+LVVHDFPYKITSI SQ++D KIVLFSNQEE 
Sbjct: 181 HIHVTKLCSNALALVQKLTTDIAITDEKSLVVHDFPYKITSIPSQMDDPKIVLFSNQEED 240

Query: 241 ENGRREDLENGIKWPEWMSIQDQKLLESSSSEAAADVVVAVDGSGNYKTVSEAVEAAPSK 300
           EN RRE+LE+GIKWP+WMSI+DQKLLESSS EAAA+ VVA DGSGNYKTV+EAV AAPSK
Sbjct: 241 ENRRREELEDGIKWPKWMSIEDQKLLESSS-EAAAEAVVAADGSGNYKTVAEAVAAAPSK 300

Query: 301 NSKRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATVVAV 360
           NSKRYIIKIKAG Y ENVDVPSSKRNIMFWGDGRSNT I + RSHG+GWSTF SAT+ AV
Sbjct: 301 NSKRYIIKIKAGEYWENVDVPSSKRNIMFWGDGRSNTKIISNRSHGTGWSTFKSATLAAV 360

Query: 361 GDGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVNCVV 420
           GDGFLARDI+F+N AG ANGQAVALRVGSDHSAFYRC ML YQDTLYVHSNRQFFVNC+V
Sbjct: 361 GDGFLARDITFQNKAGAANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFFVNCIV 420

Query: 421 AGTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKATSDL 480
           AGTVDF+FGNAAAV QNSD+TPRKPGP+QRNMVTAQSRTD+NQNTGIVIQKCRIKATSDL
Sbjct: 421 AGTVDFIFGNAAAVFQNSDITPRKPGPSQRNMVTAQSRTDINQNTGIVIQKCRIKATSDL 480

Query: 481 EPVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGDIKKPYFAEYDNNGAGAN 540
           EPVI+EFPSFLGRPWEEYARVVVMQTTI+NVIDK+GWSTWNG  K PY+AEYDNNGAGA+
Sbjct: 481 EPVIEEFPSFLGRPWEEYARVVVMQTTISNVIDKEGWSTWNGQRKSPYYAEYDNNGAGAD 540

Query: 541 TSGRVPWSLVINEADAKTFTAERFIGGADWLPSTGFPYQLGLY 584
            SGRVPWSLVI+EA AKTFTA  FIGGADWL STGFPYQL LY
Sbjct: 541 ISGRVPWSLVIDEAQAKTFTAGPFIGGADWLSSTGFPYQLSLY 574

BLAST of MELO3C021067 vs. TrEMBL
Match: A0A0A0LCR6_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_3G646640 PE=4 SV=1)

HSP 1 Score: 602.4 bits (1552), Expect = 5.7e-169
Identity = 317/585 (54.19%), Postives = 416/585 (71.11%), Query Frame = 1

Query: 4   TKLSLALFATLLLTNAIIVRANSNENGVMDNISSLIETSCAITLYPQLCHSTISSIIGTS 63
           +K +L +FA  L T  ++   +S+++         + + CA+TLYP+LC +TIS+ +G+S
Sbjct: 18  SKTNLLVFA--LSTTLLLAVVSSDDH---------VGSKCAMTLYPELCETTISTAVGSS 77

Query: 64  NLLSLKDIVQLSLSVAMDAAKLNNGNIKKNLTMSFNNVSKRDRIPLHDCVKSTDRTIYEL 123
           +    K+ ++ S+++ + A K N   ++K L  +  N++KR +I  HDC+++ + T+ EL
Sbjct: 78  S----KEAIEASVNITISAVKDNYKRVQK-LLKTVKNLTKRQKIAFHDCLETGEETLREL 137

Query: 124 DKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKGVLRVIENTHIH 183
            + +ED  EYP KKSL+ YADDLKT LSS ITNQ TC+DGF  DK +K V   ++   IH
Sbjct: 138 YEVVEDVNEYPKKKSLSRYADDLKTLLSSTITNQETCVDGFSHDKGDKKVRESLKEGLIH 197

Query: 184 ATKLCSNALALVKKLT-TEIALIDEKNLVVHDFPYKITSISSQINDLKIVLFSNQEEGEN 243
             KLCS ALAL+K LT T+IA ++  N   H                      N+++ E 
Sbjct: 198 IEKLCSIALALIKNLTDTDIANLNNNNNNNH---------------------LNRKQLEE 257

Query: 244 GRREDLENGIKWPEWMSIQDQKLLESSSSEAAADVVVAVDGSGNYKTVSEAVEAAPSKNS 303
            + ED   GIKWP+WMS +D++LL++SS+ A  DVVVA DGSG+++T+SEAV AAPS++S
Sbjct: 258 KKMED---GIKWPDWMSPKDRRLLQASST-ATPDVVVAADGSGDFRTISEAVAAAPSRSS 317

Query: 304 KRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATVVAVGD 363
           +RYII+IKAGVYRENV+V SSKRNIMFWGDGR NTIIT  R+   G +TFNSATV AVG+
Sbjct: 318 RRYIIRIKAGVYRENVNVASSKRNIMFWGDGRVNTIITGNRNVVDGSTTFNSATVAAVGE 377

Query: 364 GFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVNCVVAG 423
            FLARD++F+NTAGP+  QAVALRVGSD SAFYRC ML YQDTLYVHSNRQF+V C++ G
Sbjct: 378 RFLARDVTFQNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVG 437

Query: 424 TVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKATSDLEP 483
           T+DF+FGNAAAVIQ+ D+  R+P P QRNMVTAQ RTD NQNTGIVIQKCRI  TSDL P
Sbjct: 438 TIDFIFGNAAAVIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRP 497

Query: 484 VIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGD--IKKPYFAEYDNNGAGAN 543
           VI  FP+FLGRPW+ Y+R VVMQT+I+NVID  GW  W+G+  +   ++AEY N+GAGA+
Sbjct: 498 VISNFPTFLGRPWQRYSRTVVMQTSISNVIDPAGWHVWDGNFALDTLFYAEYQNSGAGAD 557

Query: 544 TSGRVPWS---LVINEADAKTFTAERFIGGADWLPSTGFPYQLGL 583
           TS RV W    ++   A+A+ FTA  FIGG  WL STGFP+ LGL
Sbjct: 558 TSRRVKWKGFRVLTRAAEAEAFTAGNFIGGGTWLSSTGFPFSLGL 561

BLAST of MELO3C021067 vs. TrEMBL
Match: A0A067KWU1_JATCU (Pectinesterase OS=Jatropha curcas GN=JCGZ_24626 PE=4 SV=1)

HSP 1 Score: 597.8 bits (1540), Expect = 1.4e-167
Identity = 314/592 (53.04%), Postives = 416/592 (70.27%), Query Frame = 1

Query: 1   MENTKLSLALFATLLLTN---AIIVRANSNENGVMDNISSLIETSCAITLYPQLCHSTIS 60
           M+  KL L+LFA+LL+     AI+   NSN+        +++++SC+ T +P+LC S ++
Sbjct: 21  MKRKKLFLSLFASLLIVATIIAIVTGVNSNKKDNTSQSHAILKSSCSSTRFPELCFSAVA 80

Query: 61  SIIG-TSNLLSLKDIVQLSLSVAMDAAKLNNGNIKKNLTMSFNNVSKRDRIPLHDCVKST 120
           S+ G T+NL S KD+++LS+++   A + N   ++K   ++   ++KR++  LHDC+++ 
Sbjct: 81  SVPGATANLASQKDVIELSINLTTTAVQHNFFTVEK--LIATRKLTKREKTALHDCLETI 140

Query: 121 DRTIYELDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKGVLRV 180
           D T+ EL KA ED  EYPNKKSL+ +ADDLKT LSSAITNQ TCLDGF  D+ +K +   
Sbjct: 141 DETLDELHKAAEDLEEYPNKKSLSQHADDLKTLLSSAITNQETCLDGFSHDEADKRIREA 200

Query: 181 IENTHIHATKLCSNALALVKKLT-TEIALIDEKNLVVHDFPYKITSISSQINDLKIVLFS 240
           + +  +H  K+CSNALA++K +T T+IA                       ++LK+   +
Sbjct: 201 LLDGQVHVEKMCSNALAMIKNMTDTDIA-----------------------DELKLAATT 260

Query: 241 NQEEGENGRREDLENGIKWPEWMSIQDQKLLESSSSEAAADVVVAVDGSGNYKTVSEAVE 300
           N++  E G+ +  E+G  WPEW+S  D++LL+SSS     DVVVA DGSG YK + +AV 
Sbjct: 261 NRKLKEEGKNQ--ESG--WPEWLSAGDRRLLQSSS--VTPDVVVAADGSGKYKKIQDAVN 320

Query: 301 AAPSKNSKRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSA 360
           AAPSK+SKRYII+IKAGVYRENV+VP SK NIMF GDGR  TIITA R+   G +TFNSA
Sbjct: 321 AAPSKSSKRYIIRIKAGVYRENVEVPKSKTNIMFLGDGRKTTIITASRNVADGSTTFNSA 380

Query: 361 TVVAVGDGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFF 420
           TV AVG GFLA+DI+FENTAGP+  QAVALRVGSD SAFY+C ML YQDTLYVHSNRQFF
Sbjct: 381 TVAAVGQGFLAKDITFENTAGPSKHQAVALRVGSDLSAFYQCDMLAYQDTLYVHSNRQFF 440

Query: 421 VNCVVAGTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIK 480
           +NC+VAGTVDF+FGNAAAV+Q+ D+  RKP   Q+NMVTAQ RTD NQNTGIVIQK RI 
Sbjct: 441 INCLVAGTVDFIFGNAAAVLQDCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIG 500

Query: 481 ATSDLEPVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGD--IKKPYFAEYD 540
           ATSDL+PV   FP++LGRPW+EY+R V+MQ+TI++VI   GW  W GD  +    + EY 
Sbjct: 501 ATSDLKPVQSSFPTYLGRPWKEYSRTVIMQSTISDVIHPAGWHLWTGDFALNTLTYREYQ 560

Query: 541 NNGAGANTSGRVPW---SLVINEADAKTFTAERFIGGADWLPSTGFPYQLGL 583
           N+GAGA TS RV W    ++ + ++A+ FT   FI G++WL  T FP+ LGL
Sbjct: 561 NSGAGAGTSNRVTWKGYKVITSASEAQAFTPGNFIAGSNWLRGTTFPFSLGL 581

BLAST of MELO3C021067 vs. TrEMBL
Match: B9GXZ8_POPTR (Pectinesterase OS=Populus trichocarpa GN=POPTR_0003s07040g PE=4 SV=2)

HSP 1 Score: 590.5 bits (1521), Expect = 2.2e-165
Identity = 313/591 (52.96%), Postives = 405/591 (68.53%), Query Frame = 1

Query: 2   ENTKLSLALFATLLLTNAIIV---RANSNENGVMDNISSLIETSCAITLYPQLCHSTISS 61
           +N KL L + A+LLL  AII      NS+++   +   +++++SC+ TLYP+LC+S +++
Sbjct: 28  KNKKLFLVILASLLLVTAIIAIVAGVNSHKSSKNEGTHAILKSSCSSTLYPELCYSAVAT 87

Query: 62  IIG-TSNLLSLKDIVQLSLSVAMDAAKLNNGNIKKNLTMSFNNVSKRDRIPLHDCVKSTD 121
           + G TSNL S KD+++LS+++   A + N   ++K   ++   ++KR++  LHDC++  D
Sbjct: 88  VPGATSNLASQKDVIELSINLTTKAVQHNFFTVEK--LIATKKLTKREKTALHDCLEIID 147

Query: 122 RTIYELDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKGVLRVI 181
            T+ EL +A+ D  +YPN KSL  +ADDLKT LSSAITNQ TCLDGF  D+ +K V + +
Sbjct: 148 ETLDELHEALVDLNDYPNNKSLKKHADDLKTLLSSAITNQETCLDGFSHDEADKKVRKAL 207

Query: 182 ENTHIHATKLCSNALALVKKLT-TEIALIDEKNLVVHDFPYKITSISSQINDLKIVLFSN 241
               IH  K+CSN LA++K +T T++A                       N+LK      
Sbjct: 208 LKGQIHVEKMCSNVLAMIKNMTDTDVA-----------------------NELKTTNRKL 267

Query: 242 QEEGENGRREDLENGIKWPEWMSIQDQKLLESSSSEAAADVVVAVDGSGNYKTVSEAVEA 301
            +E E    E       WPEWMS+ D++LL+SSS     DVVVA DGSGNYKTVS AV A
Sbjct: 268 MQEKEGNESE-------WPEWMSVADRRLLQSSS--VTPDVVVAADGSGNYKTVSAAVAA 327

Query: 302 APSKNSKRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSAT 361
           AP K+SKRYII+IKAGVYRENVDVP  K NIMF GDGR  TIITA R+   G +TFNSAT
Sbjct: 328 APKKSSKRYIIRIKAGVYRENVDVPKDKTNIMFMGDGRKTTIITASRNVVDGSTTFNSAT 387

Query: 362 VVAVGDGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFV 421
           V AVG GFLAR I+F+NTAGP+  QAVALRVGSD SAFY C ML YQDTLYVHSNRQFF+
Sbjct: 388 VAAVGQGFLARGITFQNTAGPSKHQAVALRVGSDLSAFYDCDMLAYQDTLYVHSNRQFFI 447

Query: 422 NCVVAGTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKA 481
           NC+VAGTVDF+FGNAA V+Q+ D+  R+P   Q+NMVTAQ RTD NQNTGIVIQK RI A
Sbjct: 448 NCLVAGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGA 507

Query: 482 TSDLEPVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGD--IKKPYFAEYDN 541
           TSDL+PV   FP++LGRPW+EY+R V+MQ++IT+VI   GW  W+G   +   Y+AEY N
Sbjct: 508 TSDLQPVKSSFPTYLGRPWKEYSRTVIMQSSITDVIQPAGWFEWSGSFALNTLYYAEYQN 567

Query: 542 NGAGANTSGRVPWS---LVINEADAKTFTAERFIGGADWLPSTGFPYQLGL 583
           +GAGA TS RV W    ++ +  +A+ FT   FI G+ WL ST FP+ LGL
Sbjct: 568 SGAGAGTSRRVTWKGYRVITSATEAQRFTPGNFIAGSSWLRSTTFPFSLGL 584

BLAST of MELO3C021067 vs. TAIR10
Match: AT3G14310.1 (AT3G14310.1 pectin methylesterase 3)

HSP 1 Score: 531.2 bits (1367), Expect = 8.2e-151
Identity = 293/597 (49.08%), Postives = 389/597 (65.16%), Query Frame = 1

Query: 2   ENTKLSLALFATLLLTNAIIV-----RANSNENGVMDNIS-SLIETSCAITLYPQLCHST 61
           +N KL L   A  LL  A +       + +NE   +   S +++ +SC+ T YP+LC S 
Sbjct: 16  KNKKLVLLSAAVALLFVAAVAGISAGASKANEKRTLSPSSHAVLRSSCSSTRYPELCISA 75

Query: 62  ISSIIGTSNLLSLKDIVQLSLSVAMDAAKLNNGNIKKNLTMSFNNVSKRDRIPLHDCVKS 121
           + +  G   L S KD+++ S+++ + A + N   +KK L      ++ R++  LHDC+++
Sbjct: 76  VVTAGGVE-LTSQKDVIEASVNLTITAVEHNYFTVKK-LIKKRKGLTPREKTALHDCLET 135

Query: 122 TDRTIYELDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKGVLR 181
            D T+ EL + +ED   YP KK+L  +A DLKT +SSAITNQ TCLDGF  D  +K V +
Sbjct: 136 IDETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSHDDADKQVRK 195

Query: 182 VIENTHIHATKLCSNALALVKKLT-TEIALIDEKNLVVHDFPYKITSISSQINDLKIVLF 241
            +    IH   +CSNALA++K +T T+IA  ++K         KITS + ++ +      
Sbjct: 196 ALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKA--------KITSNNRKLKE------ 255

Query: 242 SNQEEGEN----GRREDLENGIKWPEWMSIQDQKLLESSSSEAAADVVVAVDGSGNYKTV 301
            NQE        G  E    G  WP W+S  D++LL+ S  +A  D  VA DGSG +KTV
Sbjct: 256 ENQETTVAVDIAGAGELDSEG--WPTWLSAGDRRLLQGSGVKA--DATVAADGSGTFKTV 315

Query: 302 SEAVEAAPSKNSKRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWS 361
           + AV AAP  ++KRY+I IKAGVYRENV+V   K+NIMF GDGR+ TIIT  R+   G +
Sbjct: 316 AAAVAAAPENSNKRYVIHIKAGVYRENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGST 375

Query: 362 TFNSATVVAVGDGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHS 421
           TF+SATV AVG+ FLARDI+F+NTAGP+  QAVALRVGSD SAFY C ML YQDTLYVHS
Sbjct: 376 TFHSATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHS 435

Query: 422 NRQFFVNCVVAGTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQ 481
           NRQFFV C++AGTVDF+FGNAA V+Q+ D+  R+P   Q+NMVTAQ RTD NQNTGIVIQ
Sbjct: 436 NRQFFVKCLIAGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQ 495

Query: 482 KCRIKATSDLEPVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGD--IKKPY 541
           KCRI ATSDL+ V   FP++LGRPW+EY++ V+MQ+ I++VI  +GWS W G   +    
Sbjct: 496 KCRIGATSDLQSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLT 555

Query: 542 FAEYDNNGAGANTSGRVPW---SLVINEADAKTFTAERFIGGADWLPSTGFPYQLGL 583
           + EY N GAGA T+ RV W    ++   A+A+ +TA +FIGG  WL STGFP+ LGL
Sbjct: 556 YREYSNTGAGAGTANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592

BLAST of MELO3C021067 vs. TAIR10
Match: AT1G53830.1 (AT1G53830.1 pectin methylesterase 2)

HSP 1 Score: 522.3 bits (1344), Expect = 3.8e-148
Identity = 285/590 (48.31%), Postives = 386/590 (65.42%), Query Frame = 1

Query: 6   LSLALFATLLLTNAIIVRA---NSNENGVMDNISS----LIETSCAITLYPQLCHSTISS 65
           LS A  A LLL + + + A   N N+N  +  +SS    ++++ C+ TLYP+LC S +++
Sbjct: 22  LSSAAIALLLLASIVGIAATTTNQNKNQKITTLSSTSHAILKSVCSSTLYPELCFSAVAA 81

Query: 66  IIGTSNLLSLKDIVQLSLSVAMDAAKLNNGNIKKNLTMSFNNVSKRDRIPLHDCVKSTDR 125
             G   L S K++++ SL++   A K N   +KK L      ++ R+   LHDC+++ D 
Sbjct: 82  T-GGKELTSQKEVIEASLNLTTKAVKHNYFAVKK-LIAKRKGLTPREVTALHDCLETIDE 141

Query: 126 TIYELDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKGVLRVIE 185
           T+ EL  A+ED  +YP +KSL  +ADDLKT +SSAITNQ TCLDGF  D  ++ V + + 
Sbjct: 142 TLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQGTCLDGFSYDDADRKVRKALL 201

Query: 186 NTHIHATKLCSNALALVKKLT-TEIALIDEKNLVVHDFPYKITSISSQINDLKIVLFSNQ 245
              +H   +CSNALA++K +T T+IA     N  + D     T+ +++   LK V     
Sbjct: 202 KGQVHVEHMCSNALAMIKNMTETDIA-----NFELRDKSSTFTNNNNR--KLKEVTGDLD 261

Query: 246 EEGENGRREDLENGIKWPEWMSIQDQKLLESSSSEAAADVVVAVDGSGNYKTVSEAVEAA 305
            +G             WP+W+S+ D++LL+ S+ +A  D  VA DGSG++ TV+ AV AA
Sbjct: 262 SDG-------------WPKWLSVGDRRLLQGSTIKA--DATVADDGSGDFTTVAAAVAAA 321

Query: 306 PSKNSKRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATV 365
           P K++KR++I IKAGVYRENV+V   K NIMF GDGR  TIIT  R+   G +TF+SATV
Sbjct: 322 PEKSNKRFVIHIKAGVYRENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHSATV 381

Query: 366 VAVGDGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVN 425
            AVG+ FLARDI+F+NTAGP+  QAVALRVGSD SAFY+C M  YQDTLYVHSNRQFFV 
Sbjct: 382 AAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQFFVK 441

Query: 426 CVVAGTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKAT 485
           C + GTVDF+FGNAAAV+Q+ D+  R+P   Q+NMVTAQ R+D NQNTGIVIQ CRI  T
Sbjct: 442 CHITGTVDFIFGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCRIGGT 501

Query: 486 SDLEPVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGD--IKKPYFAEYDNN 545
           SDL  V   FP++LGRPW+EY+R V+MQ+ I++VI  +GW  W+G   +    + EY N 
Sbjct: 502 SDLLAVKGTFPTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYREYLNR 561

Query: 546 GAGANTSGRVPW---SLVINEADAKTFTAERFIGGADWLPSTGFPYQLGL 583
           G GA T+ RV W    ++ ++ +A+ FTA +FIGG  WL STGFP+ L L
Sbjct: 562 GGGAGTANRVKWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPFSLSL 587

BLAST of MELO3C021067 vs. TAIR10
Match: AT5G53370.1 (AT5G53370.1 pectin methylesterase PCR fragment F)

HSP 1 Score: 352.4 bits (903), Expect = 5.2e-97
Identity = 214/601 (35.61%), Postives = 323/601 (53.74%), Query Frame = 1

Query: 2   ENTKLSLALFATLLLTNAII----------VRANSNENGVMDNISSLIETSCAITLYPQL 61
           + TK  L LF   +L   ++          V +   E  +    +  I  +C+ +LYP L
Sbjct: 28  KKTKTKLILFTLAVLVVGVVCFGIFAGIRAVDSGKTEPKLTRKPTQAISRTCSKSLYPNL 87

Query: 62  CHSTISSIIGTSNLLSLKDIVQLSLSVAMDAAKLNNGNIKKNLTMSFNNVSKRDRIPLHD 121
           C  T+    G S      +++ +S +  +   K +   +  + T+++  +  R R     
Sbjct: 88  CIDTLLDFPG-SLTADENELIHISFNATLQ--KFSKA-LYTSSTITYTQMPPRVRSAYDS 147

Query: 122 CVKSTDRTIYELDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEK 181
           C++  D ++  L +A+         +S +    D+ T+LSSA+TN  TC DGF + + + 
Sbjct: 148 CLELLDDSVDALTRALSSVVVVSGDESHS----DVMTWLSSAMTNHDTCTDGFDEIEGQG 207

Query: 182 GVLR-VIENTHIHATKLCSNALALVKKLTTEIALIDEKNLVVHDFPYKITSISS--QIND 241
           G ++  +       +++ SN LA+                    F  K+  +S    +N+
Sbjct: 208 GEVKDQVIGAVKDLSEMVSNCLAI--------------------FAGKVKDLSGVPVVNN 267

Query: 242 LKIVLFSNQEEGENG-RREDLENGIKWPEWMSIQDQKLLESSSSEAAADVVVAVDGSGNY 301
            K++     EE  N  +RED E               LL + +S   AD+ V+ DGSG +
Sbjct: 268 RKLLGTEETEELPNWLKREDRE---------------LLGTPTSAIQADITVSKDGSGTF 327

Query: 302 KTVSEAVEAAPSKNSKRYIIKIKAGVY-RENVDVPSSKRNIMFWGDGRSNTIITAYRSHG 361
           KT++EA++ AP  +S+R++I +KAG Y  EN+ V   K N+MF GDG+  T+IT  +S  
Sbjct: 328 KTIAEAIKKAPEHSSRRFVIYVKAGRYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIA 387

Query: 362 SGWSTFNSATVVAVGDGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTL 421
              +TF++AT  A G GF+ RD++FEN AGPA  QAVALRVG DH+  YRC ++GYQD L
Sbjct: 388 DDLTTFHTATFAATGAGFIVRDMTFENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDAL 447

Query: 422 YVHSNRQFFVNCVVAGTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTG 481
           YVHSNRQFF  C + GTVDF+FGNAA ++Q+ ++  RKP   Q+  +TAQ+R D NQNTG
Sbjct: 448 YVHSNRQFFRECEIYGTVDFIFGNAAVILQSCNIYARKPMAQQKITITAQNRKDPNQNTG 507

Query: 482 IVIQKCRIKATSDLEPVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGD--I 541
           I I  C++ AT DLE     +P++LGRPW+ Y+RVV M + + + ID +GW  WNG   +
Sbjct: 508 ISIHACKLLATPDLEASKGSYPTYLGRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFAL 567

Query: 542 KKPYFAEYDNNGAGANTSGRVPW---SLVINEADAKTFTAERFIGGADWLPSTGFPYQLG 583
              Y+ EY N G G+    RV W    ++ +  +A  FT  +FI G+ WLPSTG  +  G
Sbjct: 568 DSLYYGEYMNKGLGSGIGQRVKWPGYHVITSTVEASKFTVAQFISGSSWLPSTGVSFFSG 585

BLAST of MELO3C021067 vs. TAIR10
Match: AT1G23200.1 (AT1G23200.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 341.3 bits (874), Expect = 1.2e-93
Identity = 211/558 (37.81%), Postives = 302/558 (54.12%), Query Frame = 1

Query: 41  TSCAITLYPQLC-----HSTISSIIGTSNLLSLKDIVQLSLSVAMDAAKLNNGNIKKNLT 100
           TSC  T YP +C     +S + ++   ++  +  D+V   +S  MD A   +  +     
Sbjct: 36  TSCKQTPYPSVCDHHMSNSPLKTLDDQTDGFTFHDLV---VSSTMDQAVQLHRLVSS--L 95

Query: 101 MSFNNVSKRDRIPLHDCVKSTDRTIYELDKAIEDFREYPNKKSLTSYADDLKTFLSSAIT 160
              +++ K     L DC++  + TI +L+ +   + +Y       S   D +T LS+AI 
Sbjct: 96  KQHHSLHKHATSALFDCLELYEDTIDQLNHSRRSYGQY-------SSPHDRQTSLSAAIA 155

Query: 161 NQVTCLDGFQDDKTEKGVLRVIE-NTHIHATKLCSNALALVKKLTTEIALIDEKNLVVHD 220
           NQ TC +GF+D K      +      H + TK  SN+LA V K   E   + EK      
Sbjct: 156 NQDTCRNGFRDFKLTSSYSKYFPVQFHRNLTKSISNSLA-VTKAAAEAEAVAEK------ 215

Query: 221 FPYKITSISSQINDLKIVLFSNQEEGENG---RREDLENGIKWPEWMSIQDQKLLESSSS 280
             Y  T  +          FS Q     G   RR  L +  K+P W  + D+KLLE S +
Sbjct: 216 --YPSTGFTK---------FSKQRSSAGGGSHRRLLLFSDEKFPSWFPLSDRKLLEDSKT 275

Query: 281 EAAADVVVAVDGSGNYKTVSEAVEAAPS--KNSKRYIIKIKAGVYRENVDVPSSKRNIMF 340
            A AD+VVA DGSG+Y ++ +AV AA    + ++R +I +KAGVYRENV +  S +N+M 
Sbjct: 276 TAKADLVVAKDGSGHYTSIQQAVNAAAKLPRRNQRLVIYVKAGVYRENVVIKKSIKNVMV 335

Query: 341 WGDGRSNTIITAYRSHGSGWSTFNSATVVAVGDGFLARDISFENTAGPANGQAVALRVGS 400
            GDG  +TI+T  R+   G +TF SAT    G+GF+A+ I+FENTAGP   QAVALR  S
Sbjct: 336 IGDGIDSTIVTGNRNVQDGTTTFRSATFAVSGNGFIAQGITFENTAGPEKHQAVALRSSS 395

Query: 401 DHSAFYRCGMLGYQDTLYVHSNRQFFVNCVVAGTVDFVFGNAAAVIQNSDLTPRKPGPNQ 460
           D S FY C   GYQDTLY+HS+RQF  NC + GTVDF+FG+A A++QN ++  RKP   Q
Sbjct: 396 DFSVFYACSFKGYQDTLYLHSSRQFLRNCNIYGTVDFIFGDATAILQNCNIYARKPMSGQ 455

Query: 461 RNMVTAQSRTDLNQNTGIVIQKCRIKATSDLEPVIKEFPSFLGRPWEEYARVVVMQTTIT 520
           +N +TAQSR + ++ TG VIQ   +   S+         ++LGRPW  ++R V M+  + 
Sbjct: 456 KNTITAQSRKEPDETTGFVIQSSTVATASE---------TYLGRPWRSHSRTVFMKCNLG 515

Query: 521 NVIDKKGWSTWNGD--IKKPYFAEYDNNGAGANTSGRVPW---SLVINEADAKTFTAERF 580
            ++   GW  W+G   +   Y+ EY N GAGA+ SGRV W    ++    +A+ FT E F
Sbjct: 516 ALVSPAGWLPWSGSFALSTLYYGEYGNTGAGASVSGRVKWPGYHVIKTVTEAEKFTVENF 554

Query: 581 IGGADWLPSTGFPYQLGL 583
           + G  W+ +TG P   GL
Sbjct: 576 LDGNYWITATGVPVNDGL 554

BLAST of MELO3C021067 vs. TAIR10
Match: AT3G05620.1 (AT3G05620.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 337.0 bits (863), Expect = 2.3e-92
Identity = 210/597 (35.18%), Postives = 312/597 (52.26%), Query Frame = 1

Query: 8   LALFATLLLTNAIIVRANSNENGVM-----DNISSLIETSCAITLYPQLCHSTISSIIGT 67
           + +   LLL   + V  +S E  ++     DN  SL+  +C      +LC S I + +  
Sbjct: 1   MGITTALLLVMLMSVHTSSYETTILKPYKEDNFRSLVAKACQFIDAHELCVSNIWTHVKE 60

Query: 68  SNL-LSLKDIVQLSLSVAMDAAKLNNGNIKKNLTMSFNNVSKRDRIPLHDCVKSTDRTIY 127
           S   L+   +++ ++  A D AKL    I   + +S   +  R+++ + DC +    ++ 
Sbjct: 61  SGHGLNPHSVLRAAVKEAHDKAKLAMERIPTVMMLS---IRSREQVAIEDCKELVGFSVT 120

Query: 128 ELDKAIEDFREY---------PNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKG 187
           EL  ++ +  +                 +   +LKT+LS+A++NQ TCL+G         
Sbjct: 121 ELAWSMLEMNKLHGGGGIDLDDGSHDAAAAGGNLKTWLSAAMSNQDTCLEG--------- 180

Query: 188 VLRVIENTHIHATKLCSNALALVKKLTTEIALIDEKNLVVHDFPYKITSISSQINDLKIV 247
                E T     +L   +L  V +L + +                   + +Q+N L   
Sbjct: 181 ----FEGTERKYEELIKGSLRQVTQLVSNVL-----------------DMYTQLNALPFK 240

Query: 248 LFSNQEEGENGRREDLENGIKWPEWMSIQDQKLL-ESSSSEAAADVVVAVDGSGNYKTVS 307
              N            E+ I  PEW++  D+ L+     S    + VVA+DG G Y+T++
Sbjct: 241 ASRN------------ESVIASPEWLTETDESLMMRHDPSVMHPNTVVAIDGKGKYRTIN 300

Query: 308 EAVEAAPSKNSKRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWST 367
           EA+  AP+ ++KRY+I +K GVY+EN+D+   K NIM  GDG   TIIT  R+   G +T
Sbjct: 301 EAINEAPNHSTKRYVIYVKKGVYKENIDLKKKKTNIMLVGDGIGQTIITGDRNFMQGLTT 360

Query: 368 FNSATVVAVGDGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSN 427
           F +ATV   G GF+A+DI+F NTAGP N QAVALRV SD SAFYRC + GYQDTLY HS 
Sbjct: 361 FRTATVAVSGRGFIAKDITFRNTAGPQNRQAVALRVDSDQSAFYRCSVEGYQDTLYAHSL 420

Query: 428 RQFFVNCVVAGTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQK 487
           RQF+ +C + GT+DF+FGN AAV+QN  +  R P P Q+  +TAQ R   NQNTG VIQ 
Sbjct: 421 RQFYRDCEIYGTIDFIFGNGAAVLQNCKIYTRVPLPLQKVTITAQGRKSPNQNTGFVIQN 480

Query: 488 CRIKATSDLEPVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGD--IKKPYF 547
             + AT          P++LGRPW+ Y+R V M T ++ ++  +GW  W G+  +   ++
Sbjct: 481 SYVLATQ---------PTYLGRPWKLYSRTVYMNTYMSQLVQPRGWLEWFGNFALDTLWY 540

Query: 548 AEYDNNGAGANTSGRVPWS--LVINEADAKTFTAERFIGGADWLPSTGFPYQLGLYN 585
            EY+N G G  +SGRV W    ++++  A +FT   FI G  WLP+TG  +  GL N
Sbjct: 541 GEYNNIGPGWRSSGRVKWPGYHIMDKRTALSFTVGSFIDGRRWLPATGVTFTAGLAN 543

BLAST of MELO3C021067 vs. NCBI nr
Match: gi|659116371|ref|XP_008458040.1| (PREDICTED: LOW QUALITY PROTEIN: pectinesterase-like [Cucumis melo])

HSP 1 Score: 1151.0 bits (2976), Expect = 0.0e+00
Identity = 580/584 (99.32%), Postives = 580/584 (99.32%), Query Frame = 1

Query: 1   MENTKLSLALFATLLLTNAIIVRANSNENGVMDNISSLIETSCAITLYPQLCHSTISSII 60
           MENTKLSLALFATLLLTNAIIVRANSNENGVMDNISSLIETSCAITLYPQLCHSTISSII
Sbjct: 23  MENTKLSLALFATLLLTNAIIVRANSNENGVMDNISSLIETSCAITLYPQLCHSTISSII 82

Query: 61  GTSNLLSLKDIVQLSLSVAMDAAKLNNGNIKKNLTMSFNNVSKRDRIPLHDCVKSTDRTI 120
           GTSNLLSLKDIVQLSLSVAMDAAKLNNGNIK    MSFNNVSKRDRIPLHDCVKSTDRTI
Sbjct: 83  GTSNLLSLKDIVQLSLSVAMDAAKLNNGNIKN--LMSFNNVSKRDRIPLHDCVKSTDRTI 142

Query: 121 YELDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKGVLRVIENT 180
           YELDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKGVLRVIENT
Sbjct: 143 YELDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKGVLRVIENT 202

Query: 181 HIHATKLCSNALALVKKLTTEIALIDEKNLVVHDFPYKITSISSQINDLKIVLFSNQEEG 240
           HIHATKLCSNALALVKKLTTEIALIDEKNLVVHDFPYKITSISSQINDLKIVLFSNQEEG
Sbjct: 203 HIHATKLCSNALALVKKLTTEIALIDEKNLVVHDFPYKITSISSQINDLKIVLFSNQEEG 262

Query: 241 ENGRREDLENGIKWPEWMSIQDQKLLESSSSEAAADVVVAVDGSGNYKTVSEAVEAAPSK 300
           ENGRREDLENGIKWPEWMSIQDQKLLESSSSEAAADVVVAVDGSGNYKTVSEAVEAAPSK
Sbjct: 263 ENGRREDLENGIKWPEWMSIQDQKLLESSSSEAAADVVVAVDGSGNYKTVSEAVEAAPSK 322

Query: 301 NSKRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATVVAV 360
           NSKRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATVVAV
Sbjct: 323 NSKRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATVVAV 382

Query: 361 GDGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVNCVV 420
           GDGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVNCVV
Sbjct: 383 GDGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVNCVV 442

Query: 421 AGTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKATSDL 480
           AGTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKATSDL
Sbjct: 443 AGTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKATSDL 502

Query: 481 EPVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGDIKKPYFAEYDNNGAGAN 540
           EPVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGDIKKPYFAEYDNNGAGAN
Sbjct: 503 EPVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGDIKKPYFAEYDNNGAGAN 562

Query: 541 TSGRVPWSLVINEADAKTFTAERFIGGADWLPSTGFPYQLGLYN 585
           TSGRVPWSLVINEADAKTFTAERFIGGADWLPSTGFPYQLGLYN
Sbjct: 563 TSGRVPWSLVINEADAKTFTAERFIGGADWLPSTGFPYQLGLYN 604

BLAST of MELO3C021067 vs. NCBI nr
Match: gi|778726997|ref|XP_011659194.1| (PREDICTED: pectinesterase [Cucumis sativus])

HSP 1 Score: 1034.2 bits (2673), Expect = 8.4e-299
Identity = 526/584 (90.07%), Postives = 550/584 (94.18%), Query Frame = 1

Query: 1   MENTKLSLALFATLLLTNAIIVRANSNENGVMDNISSLIETSCAITLYPQLCHSTISSII 60
           MENTKLSLALFATLLLTNAIIVRANSNENGVM+NI S+IETSCAITLYPQLCHSTISSI+
Sbjct: 1   MENTKLSLALFATLLLTNAIIVRANSNENGVMNNILSVIETSCAITLYPQLCHSTISSIL 60

Query: 61  GTSNLLSLKDIVQLSLSVAMDAAKLNNGNIKKNLTMSFNNVSKRDRIPLHDCVKSTDRTI 120
           GTSNLLSLKDIV+LSLSVAMDAAKLNN NIKK L+MS N V KRDRI LHDCVK+TDRTI
Sbjct: 61  GTSNLLSLKDIVELSLSVAMDAAKLNNENIKK-LSMSGNIVGKRDRIALHDCVKTTDRTI 120

Query: 121 YELDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKGVLRVIENT 180
           YELDKAI+DF EYPNKKSL SYADDLKTFLSSAITNQVTCLDG   DKTEK VLR+IENT
Sbjct: 121 YELDKAIQDFSEYPNKKSLASYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLRLIENT 180

Query: 181 HIHATKLCSNALALVKKLTTEIALIDEKNLVVHDFPYKITSISSQINDLKIVLFSNQEEG 240
           H   TKLCSNALALVKKLTT++AL DEK+LVVHDFPYKITSISSQI+D KIVLFSNQE+G
Sbjct: 181 HNQVTKLCSNALALVKKLTTDVALTDEKSLVVHDFPYKITSISSQIDDPKIVLFSNQEDG 240

Query: 241 ENGRREDLENGIKWPEWMSIQDQKLLESSSSEAAADVVVAVDGSGNYKTVSEAVEAAPSK 300
           E+ RREDLENGIKWPEWMSIQDQ LL+SSS EAA DVVVA DG+GNYKTVSEAV+AAPSK
Sbjct: 241 ESRRREDLENGIKWPEWMSIQDQMLLDSSSEEAA-DVVVAADGTGNYKTVSEAVKAAPSK 300

Query: 301 NSKRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATVVAV 360
           NS RYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITA RSHGSGWSTFNSATVVAV
Sbjct: 301 NS-RYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITADRSHGSGWSTFNSATVVAV 360

Query: 361 GDGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVNCVV 420
           GDGFLARDI+F+NTAG ANGQAVALRVGSDHSAFYRC ML YQDTLYVHSNRQFFV CVV
Sbjct: 361 GDGFLARDITFQNTAGSANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFFVKCVV 420

Query: 421 AGTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKATSDL 480
           AGTVDF+FGNAAAVIQNSDLTPRKPGPNQ NMVTAQSRTDLNQNTGIVIQKCRIKATSDL
Sbjct: 421 AGTVDFIFGNAAAVIQNSDLTPRKPGPNQNNMVTAQSRTDLNQNTGIVIQKCRIKATSDL 480

Query: 481 EPVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGDIKKPYFAEYDNNGAGAN 540
           EPVIKEFPSFLGRPWEEYARVVVMQT+ITNVIDK+GWSTWNGDIKKPYFAEYDNNGAGA+
Sbjct: 481 EPVIKEFPSFLGRPWEEYARVVVMQTSITNVIDKEGWSTWNGDIKKPYFAEYDNNGAGAD 540

Query: 541 TSGRVPWSLVINEADAKTFTAERFIGGADWLPSTGFPYQLGLYN 585
           TSGRV WSLVINEA+AKTFTAE FI GA WLPSTGFPYQLGLYN
Sbjct: 541 TSGRVSWSLVINEAEAKTFTAEPFIDGAGWLPSTGFPYQLGLYN 581

BLAST of MELO3C021067 vs. NCBI nr
Match: gi|778727001|ref|XP_004139620.2| (PREDICTED: pectinesterase-like [Cucumis sativus])

HSP 1 Score: 964.9 bits (2493), Expect = 6.3e-278
Identity = 488/583 (83.70%), Postives = 527/583 (90.39%), Query Frame = 1

Query: 1   MENTKLSLALFATLLLTNAIIVRANSNENGVMDNISSLIETSCAITLYPQLCHSTISSII 60
           MEN KLSLAL A LLLTNAI+VRANSNEN       S+IETSCAITLYPQLCHSTISSI+
Sbjct: 1   MENNKLSLALLAILLLTNAIVVRANSNEN-------SVIETSCAITLYPQLCHSTISSIV 60

Query: 61  GTSNLLSLKDIVQLSLSVAMDAAKLNNGNIKKNLTMSFNNVSKRDRIPLHDCVKSTDRTI 120
           GTSNLLSLKDI ++SLSVAMDAAK NN NIKK L +S NNVSKRD+I LHDCV++TDRTI
Sbjct: 61  GTSNLLSLKDIFEVSLSVAMDAAKHNNKNIKK-LMVSTNNVSKRDKIGLHDCVETTDRTI 120

Query: 121 YELDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKGVLRVIENT 180
           YEL KAIE FREYPNK+SLT YADDLKTFLSSAITNQVTCLDG   DKTEK VLR+IEN 
Sbjct: 121 YELGKAIEVFREYPNKRSLTLYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLRLIENA 180

Query: 181 HIHATKLCSNALALVKKLTTEIALIDEKNLVVHDFPYKITSISSQINDLKIVLFSNQEEG 240
           HIH TKLCSNALALV+KLTT+IA+ DEK+LVVHDFPYKITSI SQ++D KIVLFSNQEE 
Sbjct: 181 HIHVTKLCSNALALVQKLTTDIAITDEKSLVVHDFPYKITSIPSQMDDPKIVLFSNQEED 240

Query: 241 ENGRREDLENGIKWPEWMSIQDQKLLESSSSEAAADVVVAVDGSGNYKTVSEAVEAAPSK 300
           EN RRE+LE+GIKWP+WMSI+DQKLLESSS EAAA+ VVA DGSGNYKTV+EAV AAPSK
Sbjct: 241 ENRRREELEDGIKWPKWMSIEDQKLLESSS-EAAAEAVVAADGSGNYKTVAEAVAAAPSK 300

Query: 301 NSKRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATVVAV 360
           NSKRYIIKIKAG Y ENVDVPSSKRNIMFWGDGRSNT I + RSHG+GWSTF SAT+ AV
Sbjct: 301 NSKRYIIKIKAGEYWENVDVPSSKRNIMFWGDGRSNTKIISNRSHGTGWSTFKSATLAAV 360

Query: 361 GDGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVNCVV 420
           GDGFLARDI+F+N AG ANGQAVALRVGSDHSAFYRC ML YQDTLYVHSNRQFFVNC+V
Sbjct: 361 GDGFLARDITFQNKAGAANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFFVNCIV 420

Query: 421 AGTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKATSDL 480
           AGTVDF+FGNAAAV QNSD+TPRKPGP+QRNMVTAQSRTD+NQNTGIVIQKCRIKATSDL
Sbjct: 421 AGTVDFIFGNAAAVFQNSDITPRKPGPSQRNMVTAQSRTDINQNTGIVIQKCRIKATSDL 480

Query: 481 EPVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGDIKKPYFAEYDNNGAGAN 540
           EPVI+EFPSFLGRPWEEYARVVVMQTTI+NVIDK+GWSTWNG  K PY+AEYDNNGAGA+
Sbjct: 481 EPVIEEFPSFLGRPWEEYARVVVMQTTISNVIDKEGWSTWNGQRKSPYYAEYDNNGAGAD 540

Query: 541 TSGRVPWSLVINEADAKTFTAERFIGGADWLPSTGFPYQLGLY 584
            SGRVPWSLVI+EA AKTFTA  FIGGADWL STGFPYQL LY
Sbjct: 541 ISGRVPWSLVIDEAQAKTFTAGPFIGGADWLSSTGFPYQLSLY 574

BLAST of MELO3C021067 vs. NCBI nr
Match: gi|659120800|ref|XP_008460358.1| (PREDICTED: pectinesterase-like [Cucumis melo])

HSP 1 Score: 604.4 bits (1557), Expect = 2.2e-169
Identity = 316/586 (53.92%), Postives = 416/586 (70.99%), Query Frame = 1

Query: 3   NTKLSLALFATLLLTNAIIVRANSNENGVMDNISSLIETSCAITLYPQLCHSTISSIIGT 62
           N+K +L +FA  L T  ++   +S+++         + + CA+TLYP+LC +TIS+ +G+
Sbjct: 17  NSKTNLLVFA--LSTTLLLAAVSSDDH---------VRSKCAMTLYPELCETTISTAVGS 76

Query: 63  SNLLSLKDIVQLSLSVAMDAAKLNNGNIKKNLTMSFNNVSKRDRIPLHDCVKSTDRTIYE 122
           S+    K+ ++ S+++ + A   N   +KK L  + N+++KR++I LHDC+++ + T+ E
Sbjct: 77  SS----KEAIEASVNITIGAVGDNYRRVKK-LLKTENSLTKREKIALHDCLETGEETLRE 136

Query: 123 LDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKGVLRVIENTHI 182
           L + +ED  EYPNKKSL+ YADDLKT LSSAITNQ TCLDGF  DK +K V   ++   I
Sbjct: 137 LYEVVEDVNEYPNKKSLSRYADDLKTLLSSAITNQETCLDGFSHDKADKKVRESLKEGLI 196

Query: 183 HATKLCSNALALVKKLT-TEIALIDEKNLVVHDFPYKITSISSQINDLKIVLFSNQEEGE 242
           H  KLCS ALAL+K LT T+IA ++  N                          N    +
Sbjct: 197 HIEKLCSIALALIKNLTDTDIANLNNNN--------------------------NHLSRK 256

Query: 243 NGRREDLENGIKWPEWMSIQDQKLLESSSSEAAADVVVAVDGSGNYKTVSEAVEAAPSKN 302
             + ++ ++ I WP+WMS  D++LL++SS+ A  DVVVA DGSG+++T+SEAV AAPS++
Sbjct: 257 QLKEKNTDDRINWPDWMSPNDRRLLQASST-ATPDVVVAADGSGDFRTISEAVAAAPSRS 316

Query: 303 SKRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATVVAVG 362
           S+RYII+IKAGVYRENV+V SSKRNIMFWGDGR NTIIT  R+   G +TFNSATV AVG
Sbjct: 317 SQRYIIRIKAGVYRENVNVASSKRNIMFWGDGRVNTIITGNRNVVDGSTTFNSATVAAVG 376

Query: 363 DGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVNCVVA 422
           + FLARD++F+NTAGP+  QAVALRVGSD SAFYRC ML YQDTLYVHSNRQF+V C++ 
Sbjct: 377 ERFLARDVTFQNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIV 436

Query: 423 GTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKATSDLE 482
           GT+DF+FGNAAAVIQ+ D+  R+P P QRNMVTAQ RTD NQNTGIVIQKCRI  TSDL 
Sbjct: 437 GTIDFIFGNAAAVIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLR 496

Query: 483 PVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGD--IKKPYFAEYDNNGAGA 542
           PVI  FP+FLGRPW+ Y+R VVMQT+I+NVID  GW  W+G+  +   ++AEY N+GAGA
Sbjct: 497 PVISNFPTFLGRPWQRYSRTVVMQTSISNVIDPAGWHVWDGNFALDTLFYAEYQNSGAGA 556

Query: 543 NTSGRVPWS---LVINEADAKTFTAERFIGGADWLPSTGFPYQLGL 583
           +TS RV W    ++   A+A+ FTA  FIGG  WL STGFP+ LGL
Sbjct: 557 DTSRRVRWKGFRVLTRAAEAEAFTAGNFIGGGTWLSSTGFPFSLGL 559

BLAST of MELO3C021067 vs. NCBI nr
Match: gi|778682530|ref|XP_004144447.2| (PREDICTED: pectinesterase-like [Cucumis sativus])

HSP 1 Score: 602.4 bits (1552), Expect = 8.2e-169
Identity = 317/585 (54.19%), Postives = 416/585 (71.11%), Query Frame = 1

Query: 4   TKLSLALFATLLLTNAIIVRANSNENGVMDNISSLIETSCAITLYPQLCHSTISSIIGTS 63
           +K +L +FA  L T  ++   +S+++         + + CA+TLYP+LC +TIS+ +G+S
Sbjct: 18  SKTNLLVFA--LSTTLLLAVVSSDDH---------VGSKCAMTLYPELCETTISTAVGSS 77

Query: 64  NLLSLKDIVQLSLSVAMDAAKLNNGNIKKNLTMSFNNVSKRDRIPLHDCVKSTDRTIYEL 123
           +    K+ ++ S+++ + A K N   ++K L  +  N++KR +I  HDC+++ + T+ EL
Sbjct: 78  S----KEAIEASVNITISAVKDNYKRVQK-LLKTVKNLTKRQKIAFHDCLETGEETLREL 137

Query: 124 DKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKGVLRVIENTHIH 183
            + +ED  EYP KKSL+ YADDLKT LSS ITNQ TC+DGF  DK +K V   ++   IH
Sbjct: 138 YEVVEDVNEYPKKKSLSRYADDLKTLLSSTITNQETCVDGFSHDKGDKKVRESLKEGLIH 197

Query: 184 ATKLCSNALALVKKLT-TEIALIDEKNLVVHDFPYKITSISSQINDLKIVLFSNQEEGEN 243
             KLCS ALAL+K LT T+IA ++  N   H                      N+++ E 
Sbjct: 198 IEKLCSIALALIKNLTDTDIANLNNNNNNNH---------------------LNRKQLEE 257

Query: 244 GRREDLENGIKWPEWMSIQDQKLLESSSSEAAADVVVAVDGSGNYKTVSEAVEAAPSKNS 303
            + ED   GIKWP+WMS +D++LL++SS+ A  DVVVA DGSG+++T+SEAV AAPS++S
Sbjct: 258 KKMED---GIKWPDWMSPKDRRLLQASST-ATPDVVVAADGSGDFRTISEAVAAAPSRSS 317

Query: 304 KRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATVVAVGD 363
           +RYII+IKAGVYRENV+V SSKRNIMFWGDGR NTIIT  R+   G +TFNSATV AVG+
Sbjct: 318 RRYIIRIKAGVYRENVNVASSKRNIMFWGDGRVNTIITGNRNVVDGSTTFNSATVAAVGE 377

Query: 364 GFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVNCVVAG 423
            FLARD++F+NTAGP+  QAVALRVGSD SAFYRC ML YQDTLYVHSNRQF+V C++ G
Sbjct: 378 RFLARDVTFQNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVG 437

Query: 424 TVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKATSDLEP 483
           T+DF+FGNAAAVIQ+ D+  R+P P QRNMVTAQ RTD NQNTGIVIQKCRI  TSDL P
Sbjct: 438 TIDFIFGNAAAVIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRP 497

Query: 484 VIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGD--IKKPYFAEYDNNGAGAN 543
           VI  FP+FLGRPW+ Y+R VVMQT+I+NVID  GW  W+G+  +   ++AEY N+GAGA+
Sbjct: 498 VISNFPTFLGRPWQRYSRTVVMQTSISNVIDPAGWHVWDGNFALDTLFYAEYQNSGAGAD 557

Query: 544 TSGRVPWS---LVINEADAKTFTAERFIGGADWLPSTGFPYQLGL 583
           TS RV W    ++   A+A+ FTA  FIGG  WL STGFP+ LGL
Sbjct: 558 TSRRVKWKGFRVLTRAAEAEAFTAGNFIGGGTWLSSTGFPFSLGL 561