MELO3C020516.2 (gene) Melon (DHL92) v3.6.1

BLAST of MELO3C020516.2 vs. NCBI nr
Match: XP_011658753.1 (PREDICTED: cation/H(+) antiporter 24 [Cucumis sativus] >KGN65841.1 hypothetical protein Csa_1G533480 [Cucumis sativus])

HSP 1 Score: 1481.5 bits (3834), Expect = 0.0e+00
Identity = 758/830 (91.33%), Postives = 797/830 (96.02%), Query Frame = 0

Query: 1   MVRVFPVVGGNFAQYVWRNFH--GGDIISGKMVERIQVVGGVGHKTA-DGVGIACRVSRP 60
           MVRV PVVGGNFAQYVWRNFH  GGDII+GKM E IQVVGGVG KT  DG  +ACR+S P
Sbjct: 1   MVRVIPVVGGNFAQYVWRNFHGGGGDIITGKMAEGIQVVGGVGEKTGLDGPSMACRISHP 60

Query: 61  STSGDFFYGSNPLEDSFSALLLDVVFVILIIHLLHLFLRPLHQPKIVSQILGGFIIGPSV 120
            T G  FYGSNPLEDSFSALLLD+V VI IIHLLH  LRPLHQPKIVSQILGGFIIGPSV
Sbjct: 61  KTPG-VFYGSNPLEDSFSALLLDLVIVIFIIHLLHFLLRPLHQPKIVSQILGGFIIGPSV 120

Query: 121 LGHNNNFRLHLFPEDVSFLLTNIGLIGFMYFLFISGVKTDLSLIKKAGKKEYSIASFSVV 180
           LGHN NFRL+LFPEDVSFL+TNIGLIGFMYFLFISGVKTDLSLIKKAGKKEY IASFSVV
Sbjct: 121 LGHNKNFRLNLFPEDVSFLVTNIGLIGFMYFLFISGVKTDLSLIKKAGKKEYFIASFSVV 180

Query: 181 VPLLLNISFALLIRKSMNEDLAKFSSIGAITSSLAITAFPVVHPILHELNLLSSEVGRMS 240
           VPL+LNISFALLIRKSM+E+LAKFSSIGA+TSSLAITAFPVVHPILHELNLLSSEVGRMS
Sbjct: 181 VPLVLNISFALLIRKSMDENLAKFSSIGAVTSSLAITAFPVVHPILHELNLLSSEVGRMS 240

Query: 241 MSISIISDAVGINAVIAFEAAIQGETDAINSLWYLISLIVLLGFIVFGVRRAMHWIVKRT 300
           MSISIISDAVGINAVIAFEAAIQGETDA+N+LWYLISLI+LLGFIVFGVR+ MHWI+KRT
Sbjct: 241 MSISIISDAVGINAVIAFEAAIQGETDAMNALWYLISLIILLGFIVFGVRKVMHWIIKRT 300

Query: 301 PEGQAVEQGFVVAILLGVLIMGFLTDLFGIAILNGPLWLGMAIPDGPPLGSTLVERSETI 360
           PEGQAVEQGF++AILLGVL MGFLTDLFGIAILNGPLWLGMAIPDGPPLGSTLVERSETI
Sbjct: 301 PEGQAVEQGFIIAILLGVLTMGFLTDLFGIAILNGPLWLGMAIPDGPPLGSTLVERSETI 360

Query: 361 ISELLMPVSFAFVGLYTDVFEMAKAGWPTLAPLFFLALAGHFFKLGATLVASLYFQLPVR 420
           ISELLMPVSFAFVGLYTDVFEMAKAGWPTLAPLFFLALAGHFFKLGATL+ SL+FQLP+R
Sbjct: 361 ISELLMPVSFAFVGLYTDVFEMAKAGWPTLAPLFFLALAGHFFKLGATLIPSLFFQLPLR 420

Query: 421 DSLAVSFIMCLRGQVEIVLLLHWMDKKIIKIPEFTMLVLMTATVTAIVTPLVSILYDPTK 480
           DSLAVSFIMCLRGQVEI+LLLHW+DKKIIKIPEFTMLVLMTATVTAI+TPL+SILYDPTK
Sbjct: 421 DSLAVSFIMCLRGQVEIILLLHWIDKKIIKIPEFTMLVLMTATVTAILTPLISILYDPTK 480

Query: 481 PYMVNKRRTIQHLPPGTKMKIVVCIEDQGDVAALVSLLDMSNPTVVSPFSIYALHLIELV 540
           PYMV+KRRTIQHLPP TKMKIVVCIEDQ DVAALVSLLDMSNPT  SPFSIYALHLIELV
Sbjct: 481 PYMVSKRRTIQHLPPQTKMKIVVCIEDQEDVAALVSLLDMSNPTAASPFSIYALHLIELV 540

Query: 541 GRAAPVFIDHKKSKAPSKYTASDSIHNALKLYEEARSELVKLHTYTAVAPKRTMNQDICE 600
           GRAAPVFIDHKKSKAPSKYTASDSIHNALKLYEEARSELVKLHTYTAVAPKRTMNQDICE
Sbjct: 541 GRAAPVFIDHKKSKAPSKYTASDSIHNALKLYEEARSELVKLHTYTAVAPKRTMNQDICE 600

Query: 601 LGLIKRANLIILPFSRRNGEHSGVRLQNMNISVLEHAPCSVGILVDKCNLHSPMVGQSFW 660
           LGLIKRANLI LPFS RNG+HSGVRLQ+MN SVLEHAPCSVGILVDKCNLHSPMVGQ+FW
Sbjct: 601 LGLIKRANLIFLPFS-RNGQHSGVRLQDMNTSVLEHAPCSVGILVDKCNLHSPMVGQAFW 660

Query: 661 NSAQHFVVLFLGGADAREALAYADRVIGNQDVYVSVIRFLSQNSKGDNEFEKKLDDGMVT 720
           NSAQH VVLFLGGADAREALAYADRVIGNQDVYVSVIRFL+QNS+GDNEFEKKLDDGMVT
Sbjct: 661 NSAQHIVVLFLGGADAREALAYADRVIGNQDVYVSVIRFLAQNSRGDNEFEKKLDDGMVT 720

Query: 721 WFWVKNETNERVIYREVVVRNGAETIAAIQTMNDDSYDLVVVGRKQGINPVLLEGLSNWS 780
           WFWVKNETNERVIYREVVVRNGAETI AIQ++NDDSYDLV+VGRKQGINPVLLEGLSNWS
Sbjct: 721 WFWVKNETNERVIYREVVVRNGAETITAIQSLNDDSYDLVIVGRKQGINPVLLEGLSNWS 780

Query: 781 NQNELGIIGDFVASEDFTAASSILVMQQQVLRDQGQFSSGVCGKIRFDIR 828
           +QNELGI+GDFVASEDFTAASS+LV+QQQ+LRDQGQFSSG+CGKIRFDIR
Sbjct: 781 HQNELGIVGDFVASEDFTAASSVLVLQQQILRDQGQFSSGICGKIRFDIR 828

BLAST of MELO3C020516.2 vs. NCBI nr
Match: XP_008457328.1 (PREDICTED: cation/H(+) antiporter 24 isoform X1 [Cucumis melo])

HSP 1 Score: 1473.8 bits (3814), Expect = 0.0e+00
Identity = 764/764 (100.00%), Postives = 764/764 (100.00%), Query Frame = 0

Query: 64  FYGSNPLEDSFSALLLDVVFVILIIHLLHLFLRPLHQPKIVSQILGGFIIGPSVLGHNNN 123
           FYGSNPLEDSFSALLLDVVFVILIIHLLHLFLRPLHQPKIVSQILGGFIIGPSVLGHNNN
Sbjct: 12  FYGSNPLEDSFSALLLDVVFVILIIHLLHLFLRPLHQPKIVSQILGGFIIGPSVLGHNNN 71

Query: 124 FRLHLFPEDVSFLLTNIGLIGFMYFLFISGVKTDLSLIKKAGKKEYSIASFSVVVPLLLN 183
           FRLHLFPEDVSFLLTNIGLIGFMYFLFISGVKTDLSLIKKAGKKEYSIASFSVVVPLLLN
Sbjct: 72  FRLHLFPEDVSFLLTNIGLIGFMYFLFISGVKTDLSLIKKAGKKEYSIASFSVVVPLLLN 131

Query: 184 ISFALLIRKSMNEDLAKFSSIGAITSSLAITAFPVVHPILHELNLLSSEVGRMSMSISII 243
           ISFALLIRKSMNEDLAKFSSIGAITSSLAITAFPVVHPILHELNLLSSEVGRMSMSISII
Sbjct: 132 ISFALLIRKSMNEDLAKFSSIGAITSSLAITAFPVVHPILHELNLLSSEVGRMSMSISII 191

Query: 244 SDAVGINAVIAFEAAIQGETDAINSLWYLISLIVLLGFIVFGVRRAMHWIVKRTPEGQAV 303
           SDAVGINAVIAFEAAIQGETDAINSLWYLISLIVLLGFIVFGVRRAMHWIVKRTPEGQAV
Sbjct: 192 SDAVGINAVIAFEAAIQGETDAINSLWYLISLIVLLGFIVFGVRRAMHWIVKRTPEGQAV 251

Query: 304 EQGFVVAILLGVLIMGFLTDLFGIAILNGPLWLGMAIPDGPPLGSTLVERSETIISELLM 363
           EQGFVVAILLGVLIMGFLTDLFGIAILNGPLWLGMAIPDGPPLGSTLVERSETIISELLM
Sbjct: 252 EQGFVVAILLGVLIMGFLTDLFGIAILNGPLWLGMAIPDGPPLGSTLVERSETIISELLM 311

Query: 364 PVSFAFVGLYTDVFEMAKAGWPTLAPLFFLALAGHFFKLGATLVASLYFQLPVRDSLAVS 423
           PVSFAFVGLYTDVFEMAKAGWPTLAPLFFLALAGHFFKLGATLVASLYFQLPVRDSLAVS
Sbjct: 312 PVSFAFVGLYTDVFEMAKAGWPTLAPLFFLALAGHFFKLGATLVASLYFQLPVRDSLAVS 371

Query: 424 FIMCLRGQVEIVLLLHWMDKKIIKIPEFTMLVLMTATVTAIVTPLVSILYDPTKPYMVNK 483
           FIMCLRGQVEIVLLLHWMDKKIIKIPEFTMLVLMTATVTAIVTPLVSILYDPTKPYMVNK
Sbjct: 372 FIMCLRGQVEIVLLLHWMDKKIIKIPEFTMLVLMTATVTAIVTPLVSILYDPTKPYMVNK 431

Query: 484 RRTIQHLPPGTKMKIVVCIEDQGDVAALVSLLDMSNPTVVSPFSIYALHLIELVGRAAPV 543
           RRTIQHLPPGTKMKIVVCIEDQGDVAALVSLLDMSNPTVVSPFSIYALHLIELVGRAAPV
Sbjct: 432 RRTIQHLPPGTKMKIVVCIEDQGDVAALVSLLDMSNPTVVSPFSIYALHLIELVGRAAPV 491

Query: 544 FIDHKKSKAPSKYTASDSIHNALKLYEEARSELVKLHTYTAVAPKRTMNQDICELGLIKR 603
           FIDHKKSKAPSKYTASDSIHNALKLYEEARSELVKLHTYTAVAPKRTMNQDICELGLIKR
Sbjct: 492 FIDHKKSKAPSKYTASDSIHNALKLYEEARSELVKLHTYTAVAPKRTMNQDICELGLIKR 551

Query: 604 ANLIILPFSRRNGEHSGVRLQNMNISVLEHAPCSVGILVDKCNLHSPMVGQSFWNSAQHF 663
           ANLIILPFSRRNGEHSGVRLQNMNISVLEHAPCSVGILVDKCNLHSPMVGQSFWNSAQHF
Sbjct: 552 ANLIILPFSRRNGEHSGVRLQNMNISVLEHAPCSVGILVDKCNLHSPMVGQSFWNSAQHF 611

Query: 664 VVLFLGGADAREALAYADRVIGNQDVYVSVIRFLSQNSKGDNEFEKKLDDGMVTWFWVKN 723
           VVLFLGGADAREALAYADRVIGNQDVYVSVIRFLSQNSKGDNEFEKKLDDGMVTWFWVKN
Sbjct: 612 VVLFLGGADAREALAYADRVIGNQDVYVSVIRFLSQNSKGDNEFEKKLDDGMVTWFWVKN 671

Query: 724 ETNERVIYREVVVRNGAETIAAIQTMNDDSYDLVVVGRKQGINPVLLEGLSNWSNQNELG 783
           ETNERVIYREVVVRNGAETIAAIQTMNDDSYDLVVVGRKQGINPVLLEGLSNWSNQNELG
Sbjct: 672 ETNERVIYREVVVRNGAETIAAIQTMNDDSYDLVVVGRKQGINPVLLEGLSNWSNQNELG 731

Query: 784 IIGDFVASEDFTAASSILVMQQQVLRDQGQFSSGVCGKIRFDIR 828
           IIGDFVASEDFTAASSILVMQQQVLRDQGQFSSGVCGKIRFDIR
Sbjct: 732 IIGDFVASEDFTAASSILVMQQQVLRDQGQFSSGVCGKIRFDIR 775

BLAST of MELO3C020516.2 vs. NCBI nr
Match: XP_008457329.1 (PREDICTED: cation/H(+) antiporter 24 isoform X2 [Cucumis melo])

HSP 1 Score: 1467.2 bits (3797), Expect = 0.0e+00
Identity = 763/764 (99.87%), Postives = 763/764 (99.87%), Query Frame = 0

Query: 64  FYGSNPLEDSFSALLLDVVFVILIIHLLHLFLRPLHQPKIVSQILGGFIIGPSVLGHNNN 123
           FYGSNPLEDSFSALLLDVVFVILIIHLLHLFLRPLHQPKIVSQILGGFIIGPSVLGHNNN
Sbjct: 12  FYGSNPLEDSFSALLLDVVFVILIIHLLHLFLRPLHQPKIVSQILGGFIIGPSVLGHNNN 71

Query: 124 FRLHLFPEDVSFLLTNIGLIGFMYFLFISGVKTDLSLIKKAGKKEYSIASFSVVVPLLLN 183
           FRLHLFPEDVSFLLTNIGLIGFMYFLFISGVKTDLSLIKKAGKKEYSIASFSVVVPLLLN
Sbjct: 72  FRLHLFPEDVSFLLTNIGLIGFMYFLFISGVKTDLSLIKKAGKKEYSIASFSVVVPLLLN 131

Query: 184 ISFALLIRKSMNEDLAKFSSIGAITSSLAITAFPVVHPILHELNLLSSEVGRMSMSISII 243
           ISFALLIRKSMNEDLAKFSSIGAITSSLAITAFPVVHPILHELNLLSSEVGRMSMSISII
Sbjct: 132 ISFALLIRKSMNEDLAKFSSIGAITSSLAITAFPVVHPILHELNLLSSEVGRMSMSISII 191

Query: 244 SDAVGINAVIAFEAAIQGETDAINSLWYLISLIVLLGFIVFGVRRAMHWIVKRTPEGQAV 303
           SDAVGINAVIAFEAAIQGETDAINSLWYLISLIVLLGFIVFGVRRAMHWIVKRTPEGQAV
Sbjct: 192 SDAVGINAVIAFEAAIQGETDAINSLWYLISLIVLLGFIVFGVRRAMHWIVKRTPEGQAV 251

Query: 304 EQGFVVAILLGVLIMGFLTDLFGIAILNGPLWLGMAIPDGPPLGSTLVERSETIISELLM 363
           EQGFVVAILLGVLIMGFLTDLFGIAILNGPLWLGMAIPDGPPLGSTLVERSETIISELLM
Sbjct: 252 EQGFVVAILLGVLIMGFLTDLFGIAILNGPLWLGMAIPDGPPLGSTLVERSETIISELLM 311

Query: 364 PVSFAFVGLYTDVFEMAKAGWPTLAPLFFLALAGHFFKLGATLVASLYFQLPVRDSLAVS 423
           PVSFAFVGLYTDVFEMAKAGWPTLAPLFFLALAGHFFKLGATLVASLYFQLPVRDSLAVS
Sbjct: 312 PVSFAFVGLYTDVFEMAKAGWPTLAPLFFLALAGHFFKLGATLVASLYFQLPVRDSLAVS 371

Query: 424 FIMCLRGQVEIVLLLHWMDKKIIKIPEFTMLVLMTATVTAIVTPLVSILYDPTKPYMVNK 483
           FIMCLRGQVEIVLLLHWMDKKIIKIPEFTMLVLMTATVTAIVTPLVSILYDPTKPYMVNK
Sbjct: 372 FIMCLRGQVEIVLLLHWMDKKIIKIPEFTMLVLMTATVTAIVTPLVSILYDPTKPYMVNK 431

Query: 484 RRTIQHLPPGTKMKIVVCIEDQGDVAALVSLLDMSNPTVVSPFSIYALHLIELVGRAAPV 543
           RRTIQHLPPGTKMKIVVCIEDQGDVAALVSLLDMSNPTVVSPFSIYALHLIELVGRAAPV
Sbjct: 432 RRTIQHLPPGTKMKIVVCIEDQGDVAALVSLLDMSNPTVVSPFSIYALHLIELVGRAAPV 491

Query: 544 FIDHKKSKAPSKYTASDSIHNALKLYEEARSELVKLHTYTAVAPKRTMNQDICELGLIKR 603
           FIDHKKSKAPSKYTASDSIHNALKLYEEARSELVKLHTYTAVAPKRTMNQDICELGLIKR
Sbjct: 492 FIDHKKSKAPSKYTASDSIHNALKLYEEARSELVKLHTYTAVAPKRTMNQDICELGLIKR 551

Query: 604 ANLIILPFSRRNGEHSGVRLQNMNISVLEHAPCSVGILVDKCNLHSPMVGQSFWNSAQHF 663
           ANLIILPFS RNGEHSGVRLQNMNISVLEHAPCSVGILVDKCNLHSPMVGQSFWNSAQHF
Sbjct: 552 ANLIILPFS-RNGEHSGVRLQNMNISVLEHAPCSVGILVDKCNLHSPMVGQSFWNSAQHF 611

Query: 664 VVLFLGGADAREALAYADRVIGNQDVYVSVIRFLSQNSKGDNEFEKKLDDGMVTWFWVKN 723
           VVLFLGGADAREALAYADRVIGNQDVYVSVIRFLSQNSKGDNEFEKKLDDGMVTWFWVKN
Sbjct: 612 VVLFLGGADAREALAYADRVIGNQDVYVSVIRFLSQNSKGDNEFEKKLDDGMVTWFWVKN 671

Query: 724 ETNERVIYREVVVRNGAETIAAIQTMNDDSYDLVVVGRKQGINPVLLEGLSNWSNQNELG 783
           ETNERVIYREVVVRNGAETIAAIQTMNDDSYDLVVVGRKQGINPVLLEGLSNWSNQNELG
Sbjct: 672 ETNERVIYREVVVRNGAETIAAIQTMNDDSYDLVVVGRKQGINPVLLEGLSNWSNQNELG 731

Query: 784 IIGDFVASEDFTAASSILVMQQQVLRDQGQFSSGVCGKIRFDIR 828
           IIGDFVASEDFTAASSILVMQQQVLRDQGQFSSGVCGKIRFDIR
Sbjct: 732 IIGDFVASEDFTAASSILVMQQQVLRDQGQFSSGVCGKIRFDIR 774

BLAST of MELO3C020516.2 vs. NCBI nr
Match: XP_023524554.1 (cation/H(+) antiporter 24-like [Cucurbita pepo subsp. pepo] >XP_023524564.1 cation/H(+) antiporter 24-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1285.0 bits (3324), Expect = 0.0e+00
Identity = 655/832 (78.73%), Postives = 737/832 (88.58%), Query Frame = 0

Query: 1   MVRVFPVVGGNFAQY-VWRNFHGGDIISGKMVERIQVVGGV----GHKTADGVGIACRVS 60
           MVR F  +   FAQY  WRNF   D+   + ++R+QVVGG+        A    +ACR++
Sbjct: 1   MVRFFH-LPSRFAQYDNWRNFTALDL---RHIQRLQVVGGLTSFKDATPAATAPVACRLN 60

Query: 61  RPSTSGDFFYGSNPLEDSFSALLLDVVFVILIIHLLHLFLRPLHQPKIVSQILGGFIIGP 120
            P T G  FYG+NPL+DSFSAL+LD+ FVIL+IH++HL LRP HQPKIVSQILGGFIIGP
Sbjct: 61  HPRTPG-IFYGANPLDDSFSALILDISFVILLIHIIHLLLRPFHQPKIVSQILGGFIIGP 120

Query: 121 SVLGHNNNFRLHLFPEDVSFLLTNIGLIGFMYFLFISGVKTDLSLIKKAGKKEYSIASFS 180
           SVLGHN NFR  +FPED+SFLL+NIGLIGFMYFLFISGVKTDLSL KK+GKKE+ IASFS
Sbjct: 121 SVLGHNKNFRHQMFPEDISFLLSNIGLIGFMYFLFISGVKTDLSLTKKSGKKEFFIASFS 180

Query: 181 VVVPLLLNISFALLIRKSMNEDLAKFSSIGAITSSLAITAFPVVHPILHELNLLSSEVGR 240
           V+VPL+LNI+FALLIRK+MN+ LAKFSSIGAITSSLAITAFPVVHPILHELNLLSSEVGR
Sbjct: 181 VIVPLILNITFALLIRKTMNKSLAKFSSIGAITSSLAITAFPVVHPILHELNLLSSEVGR 240

Query: 241 MSMSISIISDAVGINAVIAFEAAIQGETDAINSLWYLISLIVLLGFIVFGVRRAMHWIVK 300
           MSMS+SIISDAVGINA++AFEAAIQGE +  +S+WYLISL VL+GFIV  VRR M W+V+
Sbjct: 241 MSMSVSIISDAVGINALVAFEAAIQGEVEWKSSIWYLISLFVLVGFIVVCVRRVMRWVVR 300

Query: 301 RTPEGQAVEQGFVVAILLGVLIMGFLTDLFGIAILNGPLWLGMAIPDGPPLGSTLVERSE 360
           RTPEGQAVEQGF+VAILL VL+MGFLTDLFGIAILNGPLWLGMAIPDGPPLGSTLVERSE
Sbjct: 301 RTPEGQAVEQGFIVAILLAVLVMGFLTDLFGIAILNGPLWLGMAIPDGPPLGSTLVERSE 360

Query: 361 TIISELLMPVSFAFVGLYTDVFEMAKAGWPTLAPLFFLALAGHFFKLGATLVASLYFQLP 420
           TIISELL+P SFAFVGL TDVF+M  AGW  LAPLFFLALAGHFFK G+TLVASL+FQLP
Sbjct: 361 TIISELLLPFSFAFVGLCTDVFQMVNAGWSNLAPLFFLALAGHFFKFGSTLVASLFFQLP 420

Query: 421 VRDSLAVSFIMCLRGQVEIVLLLHWMDKKIIKIPEFTMLVLMTATVTAIVTPLVSILYDP 480
           +RDSLAVS IMCLRGQVEIVLL+HW+DKKII IPEFTM++LMT  VT+I TPL+S+LYDP
Sbjct: 421 LRDSLAVSLIMCLRGQVEIVLLIHWIDKKIITIPEFTMMILMTVAVTSIATPLISLLYDP 480

Query: 481 TKPYMVNKRRTIQHLPPGTKMKIVVCIEDQGDVAALVSLLDMSNPTVVSPFSIYALHLIE 540
           T+PYM+NKRRTIQHLPPG ++++V+CIE Q +VAALV+LLDMSNPT  SPFSIYALHLIE
Sbjct: 481 TRPYMINKRRTIQHLPPGKELRVVLCIEGQENVAALVNLLDMSNPTTSSPFSIYALHLIE 540

Query: 541 LVGRAAPVFIDHKKSKAPSKYTASDSIHNALKLYEEARSELVKLHTYTAVAPKRTMNQDI 600
           LVGRAAPVFIDHKK K PSKYTASDSIHNAL++YEEAR ELVKLHTYTAVAPKR+MNQDI
Sbjct: 541 LVGRAAPVFIDHKKCKIPSKYTASDSIHNALRIYEEARGELVKLHTYTAVAPKRSMNQDI 600

Query: 601 CELGLIKRANLIILPFSRRNGEHSGVRLQNMNISVLEHAPCSVGILVDKCNLHSPMVGQS 660
           CEL LIK+ NLI+LPF R +   S V L  +N SVLEHAPCSVGILVDKCNLHSP+VGQS
Sbjct: 601 CELALIKKTNLIVLPFGRPSRGDSTVGLHQVNKSVLEHAPCSVGILVDKCNLHSPVVGQS 660

Query: 661 FWNSAQHFVVLFLGGADAREALAYADRVIGNQDVYVSVIRFLSQNSKGDNEFEKKLDDGM 720
           FWNSAQ F VLFLGGADAREALAYADR++GN+DV VSV+RFLS NS+GDNEFEKKLDDGM
Sbjct: 661 FWNSAQQFAVLFLGGADAREALAYADRILGNEDVCVSVVRFLSHNSRGDNEFEKKLDDGM 720

Query: 721 VTWFWVKNETNERVIYREVVVRNGAETIAAIQTMNDDSYDLVVVGRKQGINPVLLEGLSN 780
           VTWFWVKNETNERVIYREVVVRNGAETIAAIQ+MNDDSYDLV+VGRK+G+NPVLLEGLSN
Sbjct: 721 VTWFWVKNETNERVIYREVVVRNGAETIAAIQSMNDDSYDLVIVGRKRGVNPVLLEGLSN 780

Query: 781 WSNQNELGIIGDFVASEDFTAASSILVMQQQVLRDQGQFSSGVCGKIRFDIR 828
           WS  NELGIIGD+V+SEDF AA+S+LV+QQQVLR Q  FSSG+C K RFD R
Sbjct: 781 WSQDNELGIIGDYVSSEDFGAAASVLVVQQQVLRGQSHFSSGICEKFRFDFR 827

BLAST of MELO3C020516.2 vs. NCBI nr
Match: XP_022998645.1 (cation/H(+) antiporter 24 [Cucurbita maxima])

HSP 1 Score: 1283.9 bits (3321), Expect = 0.0e+00
Identity = 653/834 (78.30%), Postives = 736/834 (88.25%), Query Frame = 0

Query: 1   MVRVFPVVGGNFAQY-VWRNFHGGDIISGKMVERIQVVGGVGH------KTADGVGIACR 60
           MVR F  +   FAQY  WRNF   D+   + ++R+QVVGG+         +   V +ACR
Sbjct: 1   MVRFFH-LPSRFAQYDNWRNFTALDL---RHIQRLQVVGGLASFKDATPASPAAVPVACR 60

Query: 61  VSRPSTSGDFFYGSNPLEDSFSALLLDVVFVILIIHLLHLFLRPLHQPKIVSQILGGFII 120
           +  P T G  FYG+NPL+DSFSAL+LD+ FVIL+IH++HL LRP HQPKIVSQ+LGGFII
Sbjct: 61  LKHPRTPG-IFYGANPLDDSFSALILDISFVILLIHIIHLLLRPFHQPKIVSQVLGGFII 120

Query: 121 GPSVLGHNNNFRLHLFPEDVSFLLTNIGLIGFMYFLFISGVKTDLSLIKKAGKKEYSIAS 180
           GPSVL HN NFR  +FPED+SFLL+NIGLIGFMYFLFISGVKTDLSL KK+GKKE+ IAS
Sbjct: 121 GPSVLSHNKNFRHQMFPEDISFLLSNIGLIGFMYFLFISGVKTDLSLTKKSGKKEFFIAS 180

Query: 181 FSVVVPLLLNISFALLIRKSMNEDLAKFSSIGAITSSLAITAFPVVHPILHELNLLSSEV 240
           FSV+VPL+LNI+FALLIRKSMN+ LAKFSSIGAITSSLAITAFPVVHPILHELNLLSSEV
Sbjct: 181 FSVIVPLILNITFALLIRKSMNKSLAKFSSIGAITSSLAITAFPVVHPILHELNLLSSEV 240

Query: 241 GRMSMSISIISDAVGINAVIAFEAAIQGETDAINSLWYLISLIVLLGFIVFGVRRAMHWI 300
           GRMSMS+SIISDAVGINA++AFEAAIQGE +  +S+WYL+SLIVL+GFIV  VRR M W+
Sbjct: 241 GRMSMSVSIISDAVGINALVAFEAAIQGEVEWKSSIWYLVSLIVLVGFIVLCVRRIMRWV 300

Query: 301 VKRTPEGQAVEQGFVVAILLGVLIMGFLTDLFGIAILNGPLWLGMAIPDGPPLGSTLVER 360
           V+RTPEGQAVEQGF+VAILL VL+MGFLTDLFGIAILNGPLWLGMAIPDGPPLGSTLVER
Sbjct: 301 VRRTPEGQAVEQGFIVAILLAVLVMGFLTDLFGIAILNGPLWLGMAIPDGPPLGSTLVER 360

Query: 361 SETIISELLMPVSFAFVGLYTDVFEMAKAGWPTLAPLFFLALAGHFFKLGATLVASLYFQ 420
           SETIISELL+P SFAFVGL TDVF+M  AGW  LAPLFFLALAGHFFK G+TLVASLYFQ
Sbjct: 361 SETIISELLLPFSFAFVGLCTDVFQMVNAGWSNLAPLFFLALAGHFFKFGSTLVASLYFQ 420

Query: 421 LPVRDSLAVSFIMCLRGQVEIVLLLHWMDKKIIKIPEFTMLVLMTATVTAIVTPLVSILY 480
           LP+RDSLAVS IMCLRGQVEIVLLLHW+DKKII IPEFTM+++MT  VT + TPL+S+LY
Sbjct: 421 LPLRDSLAVSLIMCLRGQVEIVLLLHWIDKKIITIPEFTMMIIMTVAVTGMATPLISLLY 480

Query: 481 DPTKPYMVNKRRTIQHLPPGTKMKIVVCIEDQGDVAALVSLLDMSNPTVVSPFSIYALHL 540
           DPT+PYM+NKRRTIQHLPPG ++++V+CIE Q +VAALV+LLDMSNPT+ SPFSIYALHL
Sbjct: 481 DPTRPYMINKRRTIQHLPPGKELRVVLCIEGQENVAALVNLLDMSNPTMSSPFSIYALHL 540

Query: 541 IELVGRAAPVFIDHKKSKAPSKYTASDSIHNALKLYEEARSELVKLHTYTAVAPKRTMNQ 600
           IELVGRAAPVFIDHKK K PSKYTASDSIHNAL++YEEAR ELVKLHTYTAVAPKR+MNQ
Sbjct: 541 IELVGRAAPVFIDHKKCKIPSKYTASDSIHNALRIYEEARGELVKLHTYTAVAPKRSMNQ 600

Query: 601 DICELGLIKRANLIILPFSRRNGEHSGVRLQNMNISVLEHAPCSVGILVDKCNLHSPMVG 660
           DICEL LIK+ NLI+LPF R +   S V L  +N SVLEHAPCSVGILVDKCNLHSP+VG
Sbjct: 601 DICELALIKKTNLIVLPFGRTSTRDSTVGLHPVNKSVLEHAPCSVGILVDKCNLHSPVVG 660

Query: 661 QSFWNSAQHFVVLFLGGADAREALAYADRVIGNQDVYVSVIRFLSQNSKGDNEFEKKLDD 720
           QSFWNSAQ F VLFLGGADAREALAYADR++GNQDV VSV+RFLS NS+GDNEFEKKLDD
Sbjct: 661 QSFWNSAQQFAVLFLGGADAREALAYADRILGNQDVCVSVVRFLSHNSRGDNEFEKKLDD 720

Query: 721 GMVTWFWVKNETNERVIYREVVVRNGAETIAAIQTMNDDSYDLVVVGRKQGINPVLLEGL 780
           GMVTWFWVKNETNERVIYREVVVRNGAETIAAIQ+MNDDSYDLV+VGRK+G+NPVLLEGL
Sbjct: 721 GMVTWFWVKNETNERVIYREVVVRNGAETIAAIQSMNDDSYDLVIVGRKRGVNPVLLEGL 780

Query: 781 SNWSNQNELGIIGDFVASEDFTAASSILVMQQQVLRDQGQFSSGVCGKIRFDIR 828
           SNWS  NELGIIGD+V+SEDF AA+S+LV+ QQVLR Q  FSSG+C K RFD +
Sbjct: 781 SNWSQDNELGIIGDYVSSEDFGAAASVLVVHQQVLRGQSHFSSGICEKFRFDFQ 829

BLAST of MELO3C020516.2 vs. TAIR10
Match: AT5G37060.1 (cation/H+ exchanger 24)

HSP 1 Score: 691.0 bits (1782), Expect = 8.7e-199
Identity = 368/845 (43.55%), Postives = 534/845 (63.20%), Query Frame = 0

Query: 1   MVRVFPVVGGNFAQYVWRNFHGGDIISGKMVERIQVVGGVGHKTADGVGIACRVSRPSTS 60
           MVR FP+   +     +  + G     G++  R+          +  + + CR       
Sbjct: 1   MVRHFPINDQSLLPAHFGFWPGNSTTPGEVSNRV---------FSPKIPVVCRKLHSKQP 60

Query: 61  GDFFYGSNPLEDSFSALLLDVVFVILIIHLLHLFLRPLHQPKIVSQILGGFIIGPSVLGH 120
              F G N +  +FS  L++ + +I  I ++ + LRP  QP+IVS+I+GG +IGPS+ G 
Sbjct: 61  FGMFKGENAMNYAFSTFLIEAIIIIFFIKVVSIALRPFRQPRIVSEIIGGMMIGPSMFGG 120

Query: 121 NNNFRLHLFPEDVSFLLTNIGLIGFMYFLFISGVKTDLSLIKKAGKKEYSIASFSVVVPL 180
             NF  +LFP   +++  NIGL+GF YFLF++  KTD+  I KA +K   IA+  V+VP+
Sbjct: 121 IRNFNYYLFPPIANYICANIGLMGFFYFLFLTAAKTDVGAIGKAPRKHKYIAAIGVIVPI 180

Query: 181 LLNISFALLIRKSMNEDLAKFSSIGAITSSLAITAFPVVHPILHELNLLSSEVGRMSMSI 240
           +   S  + +R  M+E+L K SSIG +  +L+ T+FPV++ +L ++NLL+SEVG+ +MS+
Sbjct: 181 ICVGSVGMAMRDQMDENLQKPSSIGGVVFALSFTSFPVIYTVLRDMNLLNSEVGKFAMSV 240

Query: 241 SIISDAVGINAVIAFEAAIQGET-DAINSLWYLISLIVLLGFIVFGVRRAMHWIVKRTPE 300
           +++ D  G+  ++ FEA    +   A +  W+L+S+++   F++  VRRA  WIV +TPE
Sbjct: 241 ALLGDMAGVYVIVIFEAMTHADVGGAYSVFWFLVSVVIFAAFMLLVVRRAFDWIVSQTPE 300

Query: 301 GQAVEQGFVVAILLGVLIMGFLTDLFGIAILNGPLWLGMAIPDGPPLGSTLVERSETIIS 360
           G  V Q ++V IL+GVL   FLTD+FG++I  GP+WLG+ +P GPPLGSTL  RSET I 
Sbjct: 301 GTLVNQNYIVMILMGVLASCFLTDMFGLSIAVGPIWLGLLVPHGPPLGSTLAVRSETFIY 360

Query: 361 ELLMPVSFAFVGLYTDVFEMAKAGWPT-LAPLFFLALAGHFFKLGATLVASLYFQLPVRD 420
           E LMP ++A VG  T++  +    W   L+PLF++ + G   K  +T  A+L+F++P R+
Sbjct: 361 EFLMPFTYALVGQGTNIHFLRDETWRNQLSPLFYMTVVGFITKFLSTAFAALFFKVPARE 420

Query: 421 SLAVSFIMCLRGQVEIVLLLHWMDKKIIKIPEFTMLVLMTATVTAIVTPLVSILYDPTKP 480
           S+ +  +M LRGQ+++++ LHW+DK+I+  P +T++VL T  VTA+ TPL++  YDPT+P
Sbjct: 421 SITLGLMMNLRGQMDLLVYLHWIDKRIVGFPGYTVMVLHTVVVTAVTTPLINFFYDPTRP 480

Query: 481 YMVNKRRTIQHLPPGTKMKIVVCIEDQGDVAALVSLLDMSNPTVVSPFSIYALHLIELVG 540
           Y  +K RTIQH P  T+M +V+ + D   ++ L++ LD + PT  SP SI+A+ L+EL G
Sbjct: 481 YRSSKHRTIQHTPQNTEMGLVLAVSDHETLSGLITFLDFAYPTKSSPLSIFAVQLVELAG 540

Query: 541 RAAPVFIDHKKSKAPSKYTAS--------------DSIHNALKLYEEARSELVKLHTYTA 600
           RA P+FIDH+                         D + +A KLYEE R+E V L +YTA
Sbjct: 541 RATPLFIDHEXXXXXXXXXXXXXXXXXXXXXSGRIDQVQSAFKLYEEKRNECVTLRSYTA 600

Query: 601 VAPKRTMNQDICELGLIKRANLIILPFSRRNGEHSGVR------LQNMNISVLEHAPCSV 660
            APKR M QDICEL L K+   I+LP+ +   E +         + ++N  VLEH PCSV
Sbjct: 601 HAPKRLMYQDICELALGKKTAFILLPYQKERLEDAAPTELRDSGMLSVNADVLEHTPCSV 660

Query: 661 GILVDKCNLHSPMVG-----QSFWNSAQ------HFVVLFLGGADAREALAYADRVIGNQ 720
            I  DK  L + +V      Q   NS +       FVVLFLGGAD REAL  ADR+  N 
Sbjct: 661 CIYFDKGRLKNAVVRLSMDLQHSTNSIRMRQETYRFVVLFLGGADNREALHLADRMSTNP 720

Query: 721 DVYVSVIRFLSQNSKGDNEFEKKLDDGMVTWFWVKNETNERVIYREVVVRNGAETIAAIQ 780
           DV ++VIRFLS N +G++E EKKLDDG+VTWFWVKNE+NERV Y+EVVV+NGAET+AAIQ
Sbjct: 721 DVTLTVIRFLSYNHEGEDEREKKLDDGVVTWFWVKNESNERVSYKEVVVKNGAETLAAIQ 780

Query: 781 TMNDDSYDLVVVGRKQGINPVLLEGLSNWSNQNELGIIGDFVASEDFTAASSILVMQQQV 813
            MN + YDL + GR++GINP +LEGLS WS  ++LG+IGD VA+  F +  S+LV+QQQV
Sbjct: 781 AMNVNDYDLWITGRREGINPKILEGLSTWSEDHQLGVIGDTVAASVFASEGSVLVVQQQV 836

BLAST of MELO3C020516.2 vs. TAIR10
Match: AT5G58460.1 (cation/H+ exchanger 25)

HSP 1 Score: 677.9 bits (1748), Expect = 7.7e-195
Identity = 355/792 (44.82%), Postives = 514/792 (64.90%), Query Frame = 0

Query: 52  CRVSRPSTSGDFFYGSNPLEDSFSALLLDVVFVILIIHLLHLFLRPLHQPKIVSQILGGF 111
           CR          F G N +  +FS  L++ + +I  I ++++ LRPL QP+IV +I+GG 
Sbjct: 43  CRQVHDKQPFGMFKGENGMNYTFSTFLIEAILIIFFIKIVYVLLRPLRQPRIVCEIIGGM 102

Query: 112 IIGPSVLGHNNNFRLHLFPEDVSFLLTNIGLIGFMYFLFISGVKTDLSLIKKAGKKEYSI 171
           +IGPS+LG N NF  +LFP   +++  NIGL+GF YF F++  KTD++ I KA +K   I
Sbjct: 103 MIGPSMLGRNRNFNYYLFPPIANYICANIGLMGFFYFFFLTAAKTDVAEIFKAPRKHKYI 162

Query: 172 ASFSVVVPLLLNISFALLIRKSMNEDLAKFSSIGAITSSLAITAFPVVHPILHELNLLSS 231
           A+ SV+VP+    S    ++  M+  L K SSIG +T +L  T+FPV++ +L ++NLL+S
Sbjct: 163 AAVSVLVPIACVGSTGAALKHKMDIRLQKPSSIGGVTFALGFTSFPVIYTVLRDMNLLNS 222

Query: 232 EVGRMSMSISIISDAVGINAVIAFEAAIQ--GETDAINSLWYLISLIVLLGFIVFGVRRA 291
           E+G+ +MS++++ D VG+  ++ FEA  Q  G   A + +W+LIS  ++   ++  V+R+
Sbjct: 223 EIGKFAMSVTLLGDMVGVYVLVLFEAMAQADGGGGAYSVIWFLISAAIMAACLLLVVKRS 282

Query: 292 MHWIVKRTPEGQAVEQGFVVAILLGVLIMGFLTDLFGIAILNGPLWLGMAIPDGPPLGST 351
             WIV +TPEG  V Q ++V IL+GVL+  FLTD+FG+AI  GP+WLG+ +P GPPLGST
Sbjct: 283 FEWIVAKTPEGGLVNQNYIVNILMGVLVSCFLTDMFGMAIAVGPIWLGLVVPHGPPLGST 342

Query: 352 LVERSETIISELLMPVSFAFVGLYTDVFEMAKAGWP-TLAPLFFLALAGHFFKLGATLVA 411
           L  RSET ++E LMP SFA VG  T+V  ++K  WP  ++PL ++++ G   K  ++  A
Sbjct: 343 LAIRSETFVNEFLMPFSFALVGQKTNVNLISKETWPKQISPLIYMSIVGFVTKFVSSTGA 402

Query: 412 SLYFQLPVRDSLAVSFIMCLRGQVEIVLLLHWMDKKIIKIPEFTMLVLMTATVTAIVTPL 471
           +L+F++P RDSL +  +M LRGQ++I+L LHW+DK+++ +P ++++VL    VT +  PL
Sbjct: 403 ALFFKVPTRDSLTLGLMMNLRGQIDILLYLHWIDKQMVGLPGYSVMVLYAIVVTGVTAPL 462

Query: 472 VSILYDPTKPYMVNKRRTIQHLPPGTKMKIVVCIEDQGDVAALVSLLDMSNPTVVSPFSI 531
           +S LYDPT+PY  +KRRTIQH P  T+  +V+ + D    + L++ LD + PT  SPFS+
Sbjct: 463 ISFLYDPTRPYRSSKRRTIQHTPQNTETGLVLAVTDHDTFSGLITFLDFAYPTKTSPFSV 522

Query: 532 YALHLIELVGRAAPVFIDHKKSKAPSKYTAS-----------DSIHNALKLYEEARSELV 591
           +A+ L+EL GRA P+FI H K                     D + +A KLY+E RSE V
Sbjct: 523 FAIQLVELEGRAQPLFIAHDKKXXXXXXXXXXXAERMGSRRVDQVQSAFKLYQEKRSECV 582

Query: 592 KLHTYTAVAPKRTMNQDICELGLIKRANLIILPFSRRNGEHSGVR------LQNMNISVL 651
            +H YTA A K  M Q+ICEL L K+   I+LP+ +   + + +       + ++N  VL
Sbjct: 583 TMHAYTAHASKHNMYQNICELALTKKTAFILLPYQKERLQDAALTELRDSGMLSVNADVL 642

Query: 652 EHAPCSVGILVDKCNLHSPMVGQSF-----WNSAQ------HFVVLFLGGADAREALAYA 711
            H PCSV I  +K  L + MV  S       NS+        FVVLFLGGAD REAL  A
Sbjct: 643 AHTPCSVCIYYEKGRLKNAMVRSSMDPQHTTNSSHMRQEMYRFVVLFLGGADNREALHLA 702

Query: 712 DRVIGNQDVYVSVIRFLSQNSKGDNEFEKKLDDGMVTWFWVKNETNERVIYREVVVRNGA 771
           DR+  N  + ++VIRFL+ N +G++E EKKLDDG+VTWFWVKNE+N RV Y+EVVV+NGA
Sbjct: 703 DRMTENPFINLTVIRFLAHNHEGEDEREKKLDDGVVTWFWVKNESNARVSYKEVVVKNGA 762

Query: 772 ETIAAIQTMNDDSYDLVVVGRKQGINPVLLEGLSNWSNQNELGIIGDFVASEDFTAASSI 813
           ET+AAIQ MN + YDL + GR++GINP +LEGLS WS  ++LG+IGD VA   F +  S+
Sbjct: 763 ETLAAIQAMNVNDYDLWITGRREGINPKILEGLSTWSEDHQLGVIGDTVAGSVFASEGSV 822

BLAST of MELO3C020516.2 vs. TAIR10
Match: AT2G13620.1 (cation/hydrogen exchanger 15)

HSP 1 Score: 474.9 bits (1221), Expect = 9.8e-134
Identity = 283/790 (35.82%), Postives = 461/790 (58.35%), Query Frame = 0

Query: 50  IACRVSRPSTSGDFFYGSNPLEDSFSALLLDVVFVILIIHLLHLFLRPLHQPKIVSQILG 109
           I C      T+   + G NPL+ S    +L +  V+++       L+P  QP+++S+ILG
Sbjct: 13  IICYAPSMITTNGVWQGDNPLDFSLPLFVLQLTLVVVVTRFFVFILKPFRQPRVISEILG 72

Query: 110 GFIIGPSVLGHNNNFRLHLFPEDVSFLLTNIGLIGFMYFLFISGVKTDLSLIKKAGKKEY 169
           G ++GPSVLG +  F   +FP+    +L  +  +G +YFLF+ GV+ D+ +++K GK+  
Sbjct: 73  GIVLGPSVLGRSTKFAHTIFPQRSVMVLETMANVGLLYFLFLVGVEMDIMVVRKTGKRAL 132

Query: 170 SIASFSVVVPLLLNISFALLIRKSMNEDLAKFSSIGAITSSLAITAFPVVHPILHELNLL 229
           +IA   +V+P L+  +F+  + +S  + L + + I  +  +L++TAFPV+  IL EL L+
Sbjct: 133 TIAIGGMVLPFLIGAAFSFSMHRS-EDHLGQGTYILFLGVALSVTAFPVLARILAELKLI 192

Query: 230 SSEVGRMSMSISIISDAVGINAVIAFEAAIQGETDAINSLWYLISLIVLLGFIVFGVRRA 289
           ++E+GR+SMS ++++D      +    A  + +  +  SLW +IS  V +   VF VR  
Sbjct: 193 NTEIGRISMSAALVNDMFAWILLALAIALAESDKTSFASLWVMISSAVFIAVCVFVVRPG 252

Query: 290 MHWIVKRTPEGQAVEQGFVVAILLGVLIMGFLTDLFGIAILNGPLWLGMAIPDGPPLGST 349
           + WI+++TPEG+   +  +  IL GV+I GF+TD  G   + G    G+ IP+G PLG T
Sbjct: 253 IAWIIRKTPEGENFSEFHICLILTGVMISGFITDAIGTHSVFGAFVFGLVIPNG-PLGLT 312

Query: 350 LVERSETIISELLMPVSFAFVGLYTDVFEM-AKAGWPTLAPLFFLALAGHFFKLGATLVA 409
           L+E+ E  +S LL+P+ FA  GL T++  +   A W TL  + FLA AG   K+  T++ 
Sbjct: 313 LIEKLEDFVSGLLLPLFFAISGLKTNIAAIQGPATWLTLFLVIFLACAG---KVIGTVIV 372

Query: 410 SLYFQLPVRDSLAVSFIMCLRGQVEIVLLLHWMDKKIIKIPEFTMLVLMTATVTAIVTPL 469
           + +  +PVR+ + +  ++  +G VE+++L    D+K++    F  +VL+   +T ++TP+
Sbjct: 373 AFFHGMPVREGITLGLLLNTKGLVEMIVLNVGKDQKVLDDETFATMVLVALVMTGVITPI 432

Query: 470 VSILYDPTKPYMVNKRRTIQHLPPGTKMKIVVCIEDQGDVAALVSLLDMSNPTVVSPFSI 529
           V+ILY P K  +  KRRTIQ   P ++++++VC+    +V  +++LL+ S+PT  SP  I
Sbjct: 433 VTILYKPVKKSVSYKRRTIQQTKPDSELRVLVCVHTPRNVPTIINLLEASHPTKRSPICI 492

Query: 530 YALHLIELVGRAAPVFIDH--KKSKAPS---KYTASDSIHNALKLYEEARSELVKLHTYT 589
           Y LHL+EL GRA+ + I H  +KS  P+       SD I NA + YE+  +  V +   T
Sbjct: 493 YVLHLVELTGRASAMLIVHNTRKSGRPALNRTQAQSDHIINAFENYEQ-HAAFVAVQPLT 552

Query: 590 AVAPKRTMNQDICELGLIKRANLIILPFSRRNGEHSGVRLQN-----MNISVLEHAPCSV 649
           A++P  TM++D+C L   KR + II+PF ++     G+   N     +N ++LE++PCSV
Sbjct: 553 AISPYSTMHEDVCSLAEDKRVSFIIIPFHKQQTVDGGMESTNPAYRLVNQNLLENSPCSV 612

Query: 650 GILVDKCNLHSPMVGQSFWNS---AQHFVVLFLGGADAREALAYADRVIGNQDVYVSVIR 709
           GILVD+      + G +  NS   +    VLF GG D REALAYA R+  +  + ++V+R
Sbjct: 613 GILVDR-----GLNGATRLNSNTVSLQVAVLFFGGPDDREALAYAWRMAQHPGITLTVLR 672

Query: 710 FLSQNS------------------KGDNEFEKKLDDGMVTWFWVKNETNERVIYREVVVR 769
           F+                      K D+  +++LDD  +  F  +N   E ++Y E +V 
Sbjct: 673 FIHDEDEADTASTRATNDSDLKIPKMDHRKQRQLDDDYINLFRAENAEYESIVYIEKLVS 732

Query: 770 NGAETIAAIQTMNDDSYDLVVVGRKQGINPVLLEGLSNWSNQNELGIIGDFVASEDFTAA 808
           NG ET+AA+++M D S+DL +VGR +G++  L  GL++WS   ELG IGD +AS DF A 
Sbjct: 733 NGEETVAAVRSM-DSSHDLFIVGRGEGMSSPLTAGLTDWSECPELGAIGDLLASSDFAAT 790

BLAST of MELO3C020516.2 vs. TAIR10
Match: AT1G06970.1 (cation/hydrogen exchanger 14)

HSP 1 Score: 372.1 bits (954), Expect = 9.0e-103
Identity = 244/773 (31.57%), Postives = 416/773 (53.82%), Query Frame = 0

Query: 50  IACRVSRPSTSGDFFYGSNPLEDSFSALLLDVVFVILIIHLLHLFLRPLHQPKIVSQILG 109
           + C+ +   TS   F GS+PL+ +   +LL +  +I+   LL+  L+PL Q  I +Q+L 
Sbjct: 26  MVCQKNHMLTSKGVFLGSDPLKYAMPLMLLQMSVIIITSRLLYRLLKPLKQGMISAQVLA 85

Query: 110 GFIIGPSVLGHNNNFRLHLFPEDVSFLLTNIGLIGFMYFLFISGVKTDLSLIKKAGKKEY 169
           G I+GPS+ G ++ +     P      L  +  +GF   LF+ G++ D S+I+KAG K  
Sbjct: 86  GIILGPSLFGQSSAYMQMFLPISGKITLQTLSNLGFFIHLFLLGLRIDASIIRKAGSKAI 145

Query: 170 SIASFSVVVPLLLNISFALLIRKSMNEDLAKFSSIGAITSSLAITAFPVVHPILHELNLL 229
            I + S  +P  L     L ++ + N        I  + S  A+T+FPV   +L ELN+L
Sbjct: 146 LIGTASYALPFSLGNLTVLFLKNTYNLPPDVVHCISTVISLNAMTSFPVTTTVLAELNIL 205

Query: 230 SSEVGRMSMSISIISDAVGINAVIAFEAAIQGETDAINSLWYLISLIVLLGFIVFGVRRA 289
           +S++GR++ + SI+ +A      + F   ++  T  + S+W  + +  L+  I F  R A
Sbjct: 206 NSDLGRLATNCSIVCEAFSWIVALVFRMFLRDGT--LASVWSFVWVTALILVIFFVCRPA 265

Query: 290 MHWIVKRTPEG--QAVEQGFVVAILLGVLIMGFLTDLFGIAILNGPLWLGMAIPDGPPLG 349
           + W+ +R      +A E  F   I++ +L +   +++ G+    G  WLG+++PDGPPLG
Sbjct: 266 IIWLTERRSISIDKAGEIPF-FPIIMVLLTISLTSEVLGVHAAFGAFWLGVSLPDGPPLG 325

Query: 350 STLVERSETIISELLMPVSFAFVGLYTDVFEMAKAGWPTLAPLFFLALAGHFFKLGATLV 409
           + L  + E   + L++P   +  GL T+ F + ++    +  +  +     F     T  
Sbjct: 326 TGLTTKLEMFATSLMLPCFISISGLQTNFFIIGESHVKIIEAVILITYGCKFL---GTAA 385

Query: 410 ASLYFQLPVRDSLAVSFIMCLRGQVEIVLLLHWMDKKIIKIPEFTMLVLMTATVTAIVTP 469
           AS Y  + + D+ +++ +MC +G +EI   + W D+K++    F +L++    VT I   
Sbjct: 386 ASAYCNIQIGDAFSLALLMCCQGVIEIYTCVMWKDEKVLNTECFNLLIITLLLVTGISRF 445

Query: 470 LVSILYDPTKPYMVNKRRTI-QHLPPGTKMKIVVCIEDQGDVAALVSLLDMSNPTVVSPF 529
           LV  LYDP+K Y    +RTI        + ++++C+ +  +V ++V+LL+ S P+  SP 
Sbjct: 446 LVVCLYDPSKRYRSKSKRTILDTRQRNLQFRLLLCVYNVENVPSMVNLLEASYPSRFSPI 505

Query: 530 SIYALHLIELVGRAAPVFI-DHKKSKAPSKYTASDSIHNALKLYEEARSELVKLHTYTAV 589
           S++ LHL+EL GRA  V +  H+ +K       S  I N  + +E+     +    +TA 
Sbjct: 506 SVFTLHLVELKGRAHAVLVPHHQMNKLDPNTVQSTHIVNGFQRFEQQNQGTLMAQHFTAA 565

Query: 590 APKRTMNQDICELGLIKRANLIILPFSRRNG-----EHSGVRLQNMNISVLEHAPCSVGI 649
           AP  ++N DIC L L K+A LI++PF ++       +H    ++N+N++VLE APCSVGI
Sbjct: 566 APFSSINDDICTLALDKKATLIVIPFHKQYAIDGTVDHVNPSIRNINLNVLEKAPCSVGI 625

Query: 650 LVDKCNL---HSPMVGQSFWNSAQHFVVLFLGGADAREALAYADRVIGNQDVYVSVIRFL 709
            +D+       S ++  ++ N A    V+F+ G D  EALA++ R+  + +V V++I F 
Sbjct: 626 FIDRGETEGRRSVLMSYTWRNVA----VIFIEGRDDAEALAFSMRIAEHPEVSVTMIHFR 685

Query: 710 SQNSKGDN---EFEKKLDDG-MVTWFWVKNETNERVIYREVVVRNGAETIAAIQTMNDDS 769
            ++S   N   + E +L +  ++  F     +  ++ YRE +VR+G ET   I ++  DS
Sbjct: 686 HKSSLQQNHVVDVESELAESYLINDFKNFAMSKPKISYREEIVRDGVETTQVISSLG-DS 745

Query: 770 YDLVVVGRKQGINPVLLEGLSNWSNQNELGIIGDFVASEDFTAASSILVMQQQ 807
           +DLVVVGR   +   +L GL++WS   ELG+IGD  AS DF    S+LV+ QQ
Sbjct: 746 FDLVVVGRDHDLESSVLYGLTDWSECPELGVIGDMFASSDF--HFSVLVIHQQ 785

BLAST of MELO3C020516.2 vs. TAIR10
Match: AT2G30240.1 (Cation/hydrogen exchanger family protein)

HSP 1 Score: 362.8 bits (930), Expect = 5.4e-100
Identity = 245/771 (31.78%), Postives = 406/771 (52.66%), Query Frame = 0

Query: 50  IACRVSRPSTSGDFFYGSNPLEDSFSALLLDVVFVILIIHLLHLFLRPLHQPKIVSQILG 109
           I C+     TS   F  SNPL+ +   LLL +  +I+   L+   L+PL Q  I +Q+L 
Sbjct: 28  IVCQAQNMLTSRGIFMKSNPLKYALPLLLLQMSVIIVTSRLIFRVLQPLKQGMISAQVLT 87

Query: 110 GFIIGPSVLGHNNNFRLHLFPEDVSFLLTNIGLIGFMYFLFISGVKTDLSLIKKAGKKEY 169
           G ++GPS LGHN  +     P     ++  +  +GF+  LF+ G+K D S+I+KAG K  
Sbjct: 88  GVVLGPSFLGHNVIYMNMFLPAGGKIIIQTLSNVGFVIHLFLLGLKIDGSIIRKAGSKAI 147

Query: 170 SIASFSVVVPLLLNISFALLIRKSMNEDLAKFSSIGAITSSLAITAFPVVHPILHELNLL 229
            I + S   P  L     + I K+M       S   +  S  ++T+FPV   +L ELN+L
Sbjct: 148 LIGTASYAFPFSLGNLTIMFISKTMGLPSDVISCTSSAISLSSMTSFPVTTTVLAELNIL 207

Query: 230 SSEVGRMSMSISIISDAVGINAVIAFEAAIQGETDAINSLWYLISLIVLLGFIVFGVRRA 289
           +SE+GR++   S++ +       +AF    +  T  + SL+ L  +I LL  I F  R  
Sbjct: 208 NSELGRLATHCSMVCEVCSWFVALAFNLYTRDRT--MTSLYALSMIIGLLLVIYFVFRPI 267

Query: 290 MHWIVKR---TPEGQAVEQGFVVAILLGVLIMGFLTDLFGIAILNGPLWLGMAIPDGPPL 349
           + W+ +R   + + + V   F V +LL +  +    +  G+    G  WLG+++PDGPPL
Sbjct: 268 IVWLTQRKTKSMDKKDVVPFFPVLLLLSIASLS--GEAMGVHAAFGAFWLGVSLPDGPPL 327

Query: 350 GSTLVERSETIISELLMPVSFAFVGLYTDVFEMAKAGWPTLAPLFFLALAGHFFKLGATL 409
           G+ L  + E   S L +P   A  GL T+ FE+ ++    +  +  + L  +  K   T 
Sbjct: 328 GTELAAKLEMFASNLFLPCFIAISGLQTNFFEITESHEHHVVMIEIILLITYGCKFLGTA 387

Query: 410 VASLYFQLPVRDSLAVSFIMCLRGQVEIVLLLHWMDKKIIKIPEFTMLVLMTATVTAIVT 469
            AS Y Q  + D+L ++F+MC +G +E+   + W D +++    F ++++    VT I  
Sbjct: 388 AASAYCQTQIGDALCLAFLMCCQGIIEVYTTIVWKDAQVVDTECFNLVIITILFVTGISR 447

Query: 470 PLVSILYDPTKPYMVNKRRTI----QHLPPGTKMKIVVCIEDQGDVAALVSLLDMSNPTV 529
            LV  LYDP+K Y    +RTI    QH     ++++++ + +  +V ++V+LL+ + PT 
Sbjct: 448 FLVVYLYDPSKRYKSKSKRTILNTRQH---NLQLRLLLGLYNVENVPSMVNLLEATYPTR 507

Query: 530 VSPFSIYALHLIELVGRAAPVFIDHKKSKAPSKYTA-SDSIHNALKLYEEARSELVKLHT 589
            +P S + LHL+EL GRA  +   H +       TA S  I NA + +E+     +    
Sbjct: 508 FNPISFFTLHLVELKGRAHALLTPHHQMNKLDPNTAQSTHIVNAFQRFEQKYQGALMAQH 567

Query: 590 YTAVAPKRTMNQDICELGLIKRANLIILPFSRRN------GEHSGVRLQNMNISVLEHAP 649
           +TA AP  ++N DIC L L K+A LI++PF ++       G+ +G  ++ +N++VL+ AP
Sbjct: 568 FTAAAPYSSINNDICTLALDKKATLIVIPFHKQYAIDGTVGQVNG-PIRTINLNVLDAAP 627

Query: 650 CSVGILVDKCNLHSPMVGQSFWNSAQHFVVLFLGGADAREALAYADRVIGNQDVYVSVIR 709
           CSV I +D+             N+ Q+  +LF+GG D  EALA   R+    D+ V++I 
Sbjct: 628 CSVAIFIDRGETEGRR-SVLMTNTWQNVAMLFIGGKDDAEALALCMRMAEKPDLNVTMIH 687

Query: 710 FLSQNSKGDNEFEKKLDDGMVTWFWVKNETNERVIYREVVVRNGAETIAAIQTMNDDSYD 769
           F  +++  D ++    +  +++ F        ++ Y E +VR+G ET   I ++  D+YD
Sbjct: 688 FRHKSALQDEDYSDMSEYNLISDFKSYAANKGKIHYVEEIVRDGVETTQVISSLG-DAYD 747

Query: 770 LVVVGRKQGINPVLLEGLSNWSNQNELGIIGDFVASEDFTAASSILVMQQQ 807
           +V+VGR   +   +L GL++WS   ELG+IGD + S DF    S+LV+ QQ
Sbjct: 748 MVLVGRDHDLESSVLYGLTDWSECPELGVIGDMLTSPDF--HFSVLVVHQQ 786

BLAST of MELO3C020516.2 vs. Swiss-Prot
Match: sp|Q1HDT2|CHX24_ARATH (Cation/H(+) antiporter 24 OS=Arabidopsis thaliana OX=3702 GN=CHX24 PE=2 SV=2)

HSP 1 Score: 691.0 bits (1782), Expect = 1.6e-197
Identity = 368/845 (43.55%), Postives = 534/845 (63.20%), Query Frame = 0

Query: 1   MVRVFPVVGGNFAQYVWRNFHGGDIISGKMVERIQVVGGVGHKTADGVGIACRVSRPSTS 60
           MVR FP+   +     +  + G     G++  R+          +  + + CR       
Sbjct: 1   MVRHFPINDQSLLPAHFGFWPGNSTTPGEVSNRV---------FSPKIPVVCRKLHSKQP 60

Query: 61  GDFFYGSNPLEDSFSALLLDVVFVILIIHLLHLFLRPLHQPKIVSQILGGFIIGPSVLGH 120
              F G N +  +FS  L++ + +I  I ++ + LRP  QP+IVS+I+GG +IGPS+ G 
Sbjct: 61  FGMFKGENAMNYAFSTFLIEAIIIIFFIKVVSIALRPFRQPRIVSEIIGGMMIGPSMFGG 120

Query: 121 NNNFRLHLFPEDVSFLLTNIGLIGFMYFLFISGVKTDLSLIKKAGKKEYSIASFSVVVPL 180
             NF  +LFP   +++  NIGL+GF YFLF++  KTD+  I KA +K   IA+  V+VP+
Sbjct: 121 IRNFNYYLFPPIANYICANIGLMGFFYFLFLTAAKTDVGAIGKAPRKHKYIAAIGVIVPI 180

Query: 181 LLNISFALLIRKSMNEDLAKFSSIGAITSSLAITAFPVVHPILHELNLLSSEVGRMSMSI 240
           +   S  + +R  M+E+L K SSIG +  +L+ T+FPV++ +L ++NLL+SEVG+ +MS+
Sbjct: 181 ICVGSVGMAMRDQMDENLQKPSSIGGVVFALSFTSFPVIYTVLRDMNLLNSEVGKFAMSV 240

Query: 241 SIISDAVGINAVIAFEAAIQGET-DAINSLWYLISLIVLLGFIVFGVRRAMHWIVKRTPE 300
           +++ D  G+  ++ FEA    +   A +  W+L+S+++   F++  VRRA  WIV +TPE
Sbjct: 241 ALLGDMAGVYVIVIFEAMTHADVGGAYSVFWFLVSVVIFAAFMLLVVRRAFDWIVSQTPE 300

Query: 301 GQAVEQGFVVAILLGVLIMGFLTDLFGIAILNGPLWLGMAIPDGPPLGSTLVERSETIIS 360
           G  V Q ++V IL+GVL   FLTD+FG++I  GP+WLG+ +P GPPLGSTL  RSET I 
Sbjct: 301 GTLVNQNYIVMILMGVLASCFLTDMFGLSIAVGPIWLGLLVPHGPPLGSTLAVRSETFIY 360

Query: 361 ELLMPVSFAFVGLYTDVFEMAKAGWPT-LAPLFFLALAGHFFKLGATLVASLYFQLPVRD 420
           E LMP ++A VG  T++  +    W   L+PLF++ + G   K  +T  A+L+F++P R+
Sbjct: 361 EFLMPFTYALVGQGTNIHFLRDETWRNQLSPLFYMTVVGFITKFLSTAFAALFFKVPARE 420

Query: 421 SLAVSFIMCLRGQVEIVLLLHWMDKKIIKIPEFTMLVLMTATVTAIVTPLVSILYDPTKP 480
           S+ +  +M LRGQ+++++ LHW+DK+I+  P +T++VL T  VTA+ TPL++  YDPT+P
Sbjct: 421 SITLGLMMNLRGQMDLLVYLHWIDKRIVGFPGYTVMVLHTVVVTAVTTPLINFFYDPTRP 480

Query: 481 YMVNKRRTIQHLPPGTKMKIVVCIEDQGDVAALVSLLDMSNPTVVSPFSIYALHLIELVG 540
           Y  +K RTIQH P  T+M +V+ + D   ++ L++ LD + PT  SP SI+A+ L+EL G
Sbjct: 481 YRSSKHRTIQHTPQNTEMGLVLAVSDHETLSGLITFLDFAYPTKSSPLSIFAVQLVELAG 540

Query: 541 RAAPVFIDHKKSKAPSKYTAS--------------DSIHNALKLYEEARSELVKLHTYTA 600
           RA P+FIDH+                         D + +A KLYEE R+E V L +YTA
Sbjct: 541 RATPLFIDHEXXXXXXXXXXXXXXXXXXXXXSGRIDQVQSAFKLYEEKRNECVTLRSYTA 600

Query: 601 VAPKRTMNQDICELGLIKRANLIILPFSRRNGEHSGVR------LQNMNISVLEHAPCSV 660
            APKR M QDICEL L K+   I+LP+ +   E +         + ++N  VLEH PCSV
Sbjct: 601 HAPKRLMYQDICELALGKKTAFILLPYQKERLEDAAPTELRDSGMLSVNADVLEHTPCSV 660

Query: 661 GILVDKCNLHSPMVG-----QSFWNSAQ------HFVVLFLGGADAREALAYADRVIGNQ 720
            I  DK  L + +V      Q   NS +       FVVLFLGGAD REAL  ADR+  N 
Sbjct: 661 CIYFDKGRLKNAVVRLSMDLQHSTNSIRMRQETYRFVVLFLGGADNREALHLADRMSTNP 720

Query: 721 DVYVSVIRFLSQNSKGDNEFEKKLDDGMVTWFWVKNETNERVIYREVVVRNGAETIAAIQ 780
           DV ++VIRFLS N +G++E EKKLDDG+VTWFWVKNE+NERV Y+EVVV+NGAET+AAIQ
Sbjct: 721 DVTLTVIRFLSYNHEGEDEREKKLDDGVVTWFWVKNESNERVSYKEVVVKNGAETLAAIQ 780

Query: 781 TMNDDSYDLVVVGRKQGINPVLLEGLSNWSNQNELGIIGDFVASEDFTAASSILVMQQQV 813
            MN + YDL + GR++GINP +LEGLS WS  ++LG+IGD VA+  F +  S+LV+QQQV
Sbjct: 781 AMNVNDYDLWITGRREGINPKILEGLSTWSEDHQLGVIGDTVAASVFASEGSVLVVQQQV 836

BLAST of MELO3C020516.2 vs. Swiss-Prot
Match: sp|Q9FGH6|CHX25_ARATH (Cation/H(+) antiporter 25 OS=Arabidopsis thaliana OX=3702 GN=CHX25 PE=2 SV=1)

HSP 1 Score: 677.9 bits (1748), Expect = 1.4e-193
Identity = 355/792 (44.82%), Postives = 514/792 (64.90%), Query Frame = 0

Query: 52  CRVSRPSTSGDFFYGSNPLEDSFSALLLDVVFVILIIHLLHLFLRPLHQPKIVSQILGGF 111
           CR          F G N +  +FS  L++ + +I  I ++++ LRPL QP+IV +I+GG 
Sbjct: 43  CRQVHDKQPFGMFKGENGMNYTFSTFLIEAILIIFFIKIVYVLLRPLRQPRIVCEIIGGM 102

Query: 112 IIGPSVLGHNNNFRLHLFPEDVSFLLTNIGLIGFMYFLFISGVKTDLSLIKKAGKKEYSI 171
           +IGPS+LG N NF  +LFP   +++  NIGL+GF YF F++  KTD++ I KA +K   I
Sbjct: 103 MIGPSMLGRNRNFNYYLFPPIANYICANIGLMGFFYFFFLTAAKTDVAEIFKAPRKHKYI 162

Query: 172 ASFSVVVPLLLNISFALLIRKSMNEDLAKFSSIGAITSSLAITAFPVVHPILHELNLLSS 231
           A+ SV+VP+    S    ++  M+  L K SSIG +T +L  T+FPV++ +L ++NLL+S
Sbjct: 163 AAVSVLVPIACVGSTGAALKHKMDIRLQKPSSIGGVTFALGFTSFPVIYTVLRDMNLLNS 222

Query: 232 EVGRMSMSISIISDAVGINAVIAFEAAIQ--GETDAINSLWYLISLIVLLGFIVFGVRRA 291
           E+G+ +MS++++ D VG+  ++ FEA  Q  G   A + +W+LIS  ++   ++  V+R+
Sbjct: 223 EIGKFAMSVTLLGDMVGVYVLVLFEAMAQADGGGGAYSVIWFLISAAIMAACLLLVVKRS 282

Query: 292 MHWIVKRTPEGQAVEQGFVVAILLGVLIMGFLTDLFGIAILNGPLWLGMAIPDGPPLGST 351
             WIV +TPEG  V Q ++V IL+GVL+  FLTD+FG+AI  GP+WLG+ +P GPPLGST
Sbjct: 283 FEWIVAKTPEGGLVNQNYIVNILMGVLVSCFLTDMFGMAIAVGPIWLGLVVPHGPPLGST 342

Query: 352 LVERSETIISELLMPVSFAFVGLYTDVFEMAKAGWP-TLAPLFFLALAGHFFKLGATLVA 411
           L  RSET ++E LMP SFA VG  T+V  ++K  WP  ++PL ++++ G   K  ++  A
Sbjct: 343 LAIRSETFVNEFLMPFSFALVGQKTNVNLISKETWPKQISPLIYMSIVGFVTKFVSSTGA 402

Query: 412 SLYFQLPVRDSLAVSFIMCLRGQVEIVLLLHWMDKKIIKIPEFTMLVLMTATVTAIVTPL 471
           +L+F++P RDSL +  +M LRGQ++I+L LHW+DK+++ +P ++++VL    VT +  PL
Sbjct: 403 ALFFKVPTRDSLTLGLMMNLRGQIDILLYLHWIDKQMVGLPGYSVMVLYAIVVTGVTAPL 462

Query: 472 VSILYDPTKPYMVNKRRTIQHLPPGTKMKIVVCIEDQGDVAALVSLLDMSNPTVVSPFSI 531
           +S LYDPT+PY  +KRRTIQH P  T+  +V+ + D    + L++ LD + PT  SPFS+
Sbjct: 463 ISFLYDPTRPYRSSKRRTIQHTPQNTETGLVLAVTDHDTFSGLITFLDFAYPTKTSPFSV 522

Query: 532 YALHLIELVGRAAPVFIDHKKSKAPSKYTAS-----------DSIHNALKLYEEARSELV 591
           +A+ L+EL GRA P+FI H K                     D + +A KLY+E RSE V
Sbjct: 523 FAIQLVELEGRAQPLFIAHDKKXXXXXXXXXXXAERMGSRRVDQVQSAFKLYQEKRSECV 582

Query: 592 KLHTYTAVAPKRTMNQDICELGLIKRANLIILPFSRRNGEHSGVR------LQNMNISVL 651
            +H YTA A K  M Q+ICEL L K+   I+LP+ +   + + +       + ++N  VL
Sbjct: 583 TMHAYTAHASKHNMYQNICELALTKKTAFILLPYQKERLQDAALTELRDSGMLSVNADVL 642

Query: 652 EHAPCSVGILVDKCNLHSPMVGQSF-----WNSAQ------HFVVLFLGGADAREALAYA 711
            H PCSV I  +K  L + MV  S       NS+        FVVLFLGGAD REAL  A
Sbjct: 643 AHTPCSVCIYYEKGRLKNAMVRSSMDPQHTTNSSHMRQEMYRFVVLFLGGADNREALHLA 702

Query: 712 DRVIGNQDVYVSVIRFLSQNSKGDNEFEKKLDDGMVTWFWVKNETNERVIYREVVVRNGA 771
           DR+  N  + ++VIRFL+ N +G++E EKKLDDG+VTWFWVKNE+N RV Y+EVVV+NGA
Sbjct: 703 DRMTENPFINLTVIRFLAHNHEGEDEREKKLDDGVVTWFWVKNESNARVSYKEVVVKNGA 762

Query: 772 ETIAAIQTMNDDSYDLVVVGRKQGINPVLLEGLSNWSNQNELGIIGDFVASEDFTAASSI 813
           ET+AAIQ MN + YDL + GR++GINP +LEGLS WS  ++LG+IGD VA   F +  S+
Sbjct: 763 ETLAAIQAMNVNDYDLWITGRREGINPKILEGLSTWSEDHQLGVIGDTVAGSVFASEGSV 822

BLAST of MELO3C020516.2 vs. Swiss-Prot
Match: sp|Q9SIT5|CHX15_ARATH (Cation/H(+) antiporter 15 OS=Arabidopsis thaliana OX=3702 GN=CHX15 PE=2 SV=1)

HSP 1 Score: 474.9 bits (1221), Expect = 1.8e-132
Identity = 283/790 (35.82%), Postives = 461/790 (58.35%), Query Frame = 0

Query: 50  IACRVSRPSTSGDFFYGSNPLEDSFSALLLDVVFVILIIHLLHLFLRPLHQPKIVSQILG 109
           I C      T+   + G NPL+ S    +L +  V+++       L+P  QP+++S+ILG
Sbjct: 13  IICYAPSMITTNGVWQGDNPLDFSLPLFVLQLTLVVVVTRFFVFILKPFRQPRVISEILG 72

Query: 110 GFIIGPSVLGHNNNFRLHLFPEDVSFLLTNIGLIGFMYFLFISGVKTDLSLIKKAGKKEY 169
           G ++GPSVLG +  F   +FP+    +L  +  +G +YFLF+ GV+ D+ +++K GK+  
Sbjct: 73  GIVLGPSVLGRSTKFAHTIFPQRSVMVLETMANVGLLYFLFLVGVEMDIMVVRKTGKRAL 132

Query: 170 SIASFSVVVPLLLNISFALLIRKSMNEDLAKFSSIGAITSSLAITAFPVVHPILHELNLL 229
           +IA   +V+P L+  +F+  + +S  + L + + I  +  +L++TAFPV+  IL EL L+
Sbjct: 133 TIAIGGMVLPFLIGAAFSFSMHRS-EDHLGQGTYILFLGVALSVTAFPVLARILAELKLI 192

Query: 230 SSEVGRMSMSISIISDAVGINAVIAFEAAIQGETDAINSLWYLISLIVLLGFIVFGVRRA 289
           ++E+GR+SMS ++++D      +    A  + +  +  SLW +IS  V +   VF VR  
Sbjct: 193 NTEIGRISMSAALVNDMFAWILLALAIALAESDKTSFASLWVMISSAVFIAVCVFVVRPG 252

Query: 290 MHWIVKRTPEGQAVEQGFVVAILLGVLIMGFLTDLFGIAILNGPLWLGMAIPDGPPLGST 349
           + WI+++TPEG+   +  +  IL GV+I GF+TD  G   + G    G+ IP+G PLG T
Sbjct: 253 IAWIIRKTPEGENFSEFHICLILTGVMISGFITDAIGTHSVFGAFVFGLVIPNG-PLGLT 312

Query: 350 LVERSETIISELLMPVSFAFVGLYTDVFEM-AKAGWPTLAPLFFLALAGHFFKLGATLVA 409
           L+E+ E  +S LL+P+ FA  GL T++  +   A W TL  + FLA AG   K+  T++ 
Sbjct: 313 LIEKLEDFVSGLLLPLFFAISGLKTNIAAIQGPATWLTLFLVIFLACAG---KVIGTVIV 372

Query: 410 SLYFQLPVRDSLAVSFIMCLRGQVEIVLLLHWMDKKIIKIPEFTMLVLMTATVTAIVTPL 469
           + +  +PVR+ + +  ++  +G VE+++L    D+K++    F  +VL+   +T ++TP+
Sbjct: 373 AFFHGMPVREGITLGLLLNTKGLVEMIVLNVGKDQKVLDDETFATMVLVALVMTGVITPI 432

Query: 470 VSILYDPTKPYMVNKRRTIQHLPPGTKMKIVVCIEDQGDVAALVSLLDMSNPTVVSPFSI 529
           V+ILY P K  +  KRRTIQ   P ++++++VC+    +V  +++LL+ S+PT  SP  I
Sbjct: 433 VTILYKPVKKSVSYKRRTIQQTKPDSELRVLVCVHTPRNVPTIINLLEASHPTKRSPICI 492

Query: 530 YALHLIELVGRAAPVFIDH--KKSKAPS---KYTASDSIHNALKLYEEARSELVKLHTYT 589
           Y LHL+EL GRA+ + I H  +KS  P+       SD I NA + YE+  +  V +   T
Sbjct: 493 YVLHLVELTGRASAMLIVHNTRKSGRPALNRTQAQSDHIINAFENYEQ-HAAFVAVQPLT 552

Query: 590 AVAPKRTMNQDICELGLIKRANLIILPFSRRNGEHSGVRLQN-----MNISVLEHAPCSV 649
           A++P  TM++D+C L   KR + II+PF ++     G+   N     +N ++LE++PCSV
Sbjct: 553 AISPYSTMHEDVCSLAEDKRVSFIIIPFHKQQTVDGGMESTNPAYRLVNQNLLENSPCSV 612

Query: 650 GILVDKCNLHSPMVGQSFWNS---AQHFVVLFLGGADAREALAYADRVIGNQDVYVSVIR 709
           GILVD+      + G +  NS   +    VLF GG D REALAYA R+  +  + ++V+R
Sbjct: 613 GILVDR-----GLNGATRLNSNTVSLQVAVLFFGGPDDREALAYAWRMAQHPGITLTVLR 672

Query: 710 FLSQNS------------------KGDNEFEKKLDDGMVTWFWVKNETNERVIYREVVVR 769
           F+                      K D+  +++LDD  +  F  +N   E ++Y E +V 
Sbjct: 673 FIHDEDEADTASTRATNDSDLKIPKMDHRKQRQLDDDYINLFRAENAEYESIVYIEKLVS 732

Query: 770 NGAETIAAIQTMNDDSYDLVVVGRKQGINPVLLEGLSNWSNQNELGIIGDFVASEDFTAA 808
           NG ET+AA+++M D S+DL +VGR +G++  L  GL++WS   ELG IGD +AS DF A 
Sbjct: 733 NGEETVAAVRSM-DSSHDLFIVGRGEGMSSPLTAGLTDWSECPELGAIGDLLASSDFAAT 790

BLAST of MELO3C020516.2 vs. Swiss-Prot
Match: sp|Q9LMJ1|CHX14_ARATH (Cation/H(+) antiporter 14 OS=Arabidopsis thaliana OX=3702 GN=CHX14 PE=2 SV=1)

HSP 1 Score: 372.1 bits (954), Expect = 1.6e-101
Identity = 244/773 (31.57%), Postives = 416/773 (53.82%), Query Frame = 0

Query: 50  IACRVSRPSTSGDFFYGSNPLEDSFSALLLDVVFVILIIHLLHLFLRPLHQPKIVSQILG 109
           + C+ +   TS   F GS+PL+ +   +LL +  +I+   LL+  L+PL Q  I +Q+L 
Sbjct: 26  MVCQKNHMLTSKGVFLGSDPLKYAMPLMLLQMSVIIITSRLLYRLLKPLKQGMISAQVLA 85

Query: 110 GFIIGPSVLGHNNNFRLHLFPEDVSFLLTNIGLIGFMYFLFISGVKTDLSLIKKAGKKEY 169
           G I+GPS+ G ++ +     P      L  +  +GF   LF+ G++ D S+I+KAG K  
Sbjct: 86  GIILGPSLFGQSSAYMQMFLPISGKITLQTLSNLGFFIHLFLLGLRIDASIIRKAGSKAI 145

Query: 170 SIASFSVVVPLLLNISFALLIRKSMNEDLAKFSSIGAITSSLAITAFPVVHPILHELNLL 229
            I + S  +P  L     L ++ + N        I  + S  A+T+FPV   +L ELN+L
Sbjct: 146 LIGTASYALPFSLGNLTVLFLKNTYNLPPDVVHCISTVISLNAMTSFPVTTTVLAELNIL 205

Query: 230 SSEVGRMSMSISIISDAVGINAVIAFEAAIQGETDAINSLWYLISLIVLLGFIVFGVRRA 289
           +S++GR++ + SI+ +A      + F   ++  T  + S+W  + +  L+  I F  R A
Sbjct: 206 NSDLGRLATNCSIVCEAFSWIVALVFRMFLRDGT--LASVWSFVWVTALILVIFFVCRPA 265

Query: 290 MHWIVKRTPEG--QAVEQGFVVAILLGVLIMGFLTDLFGIAILNGPLWLGMAIPDGPPLG 349
           + W+ +R      +A E  F   I++ +L +   +++ G+    G  WLG+++PDGPPLG
Sbjct: 266 IIWLTERRSISIDKAGEIPF-FPIIMVLLTISLTSEVLGVHAAFGAFWLGVSLPDGPPLG 325

Query: 350 STLVERSETIISELLMPVSFAFVGLYTDVFEMAKAGWPTLAPLFFLALAGHFFKLGATLV 409
           + L  + E   + L++P   +  GL T+ F + ++    +  +  +     F     T  
Sbjct: 326 TGLTTKLEMFATSLMLPCFISISGLQTNFFIIGESHVKIIEAVILITYGCKFL---GTAA 385

Query: 410 ASLYFQLPVRDSLAVSFIMCLRGQVEIVLLLHWMDKKIIKIPEFTMLVLMTATVTAIVTP 469
           AS Y  + + D+ +++ +MC +G +EI   + W D+K++    F +L++    VT I   
Sbjct: 386 ASAYCNIQIGDAFSLALLMCCQGVIEIYTCVMWKDEKVLNTECFNLLIITLLLVTGISRF 445

Query: 470 LVSILYDPTKPYMVNKRRTI-QHLPPGTKMKIVVCIEDQGDVAALVSLLDMSNPTVVSPF 529
           LV  LYDP+K Y    +RTI        + ++++C+ +  +V ++V+LL+ S P+  SP 
Sbjct: 446 LVVCLYDPSKRYRSKSKRTILDTRQRNLQFRLLLCVYNVENVPSMVNLLEASYPSRFSPI 505

Query: 530 SIYALHLIELVGRAAPVFI-DHKKSKAPSKYTASDSIHNALKLYEEARSELVKLHTYTAV 589
           S++ LHL+EL GRA  V +  H+ +K       S  I N  + +E+     +    +TA 
Sbjct: 506 SVFTLHLVELKGRAHAVLVPHHQMNKLDPNTVQSTHIVNGFQRFEQQNQGTLMAQHFTAA 565

Query: 590 APKRTMNQDICELGLIKRANLIILPFSRRNG-----EHSGVRLQNMNISVLEHAPCSVGI 649
           AP  ++N DIC L L K+A LI++PF ++       +H    ++N+N++VLE APCSVGI
Sbjct: 566 APFSSINDDICTLALDKKATLIVIPFHKQYAIDGTVDHVNPSIRNINLNVLEKAPCSVGI 625

Query: 650 LVDKCNL---HSPMVGQSFWNSAQHFVVLFLGGADAREALAYADRVIGNQDVYVSVIRFL 709
            +D+       S ++  ++ N A    V+F+ G D  EALA++ R+  + +V V++I F 
Sbjct: 626 FIDRGETEGRRSVLMSYTWRNVA----VIFIEGRDDAEALAFSMRIAEHPEVSVTMIHFR 685

Query: 710 SQNSKGDN---EFEKKLDDG-MVTWFWVKNETNERVIYREVVVRNGAETIAAIQTMNDDS 769
            ++S   N   + E +L +  ++  F     +  ++ YRE +VR+G ET   I ++  DS
Sbjct: 686 HKSSLQQNHVVDVESELAESYLINDFKNFAMSKPKISYREEIVRDGVETTQVISSLG-DS 745

Query: 770 YDLVVVGRKQGINPVLLEGLSNWSNQNELGIIGDFVASEDFTAASSILVMQQQ 807
           +DLVVVGR   +   +L GL++WS   ELG+IGD  AS DF    S+LV+ QQ
Sbjct: 746 FDLVVVGRDHDLESSVLYGLTDWSECPELGVIGDMFASSDF--HFSVLVIHQQ 785

BLAST of MELO3C020516.2 vs. Swiss-Prot
Match: sp|O22920|CHX13_ARATH (Cation/H(+) symporter 13 OS=Arabidopsis thaliana OX=3702 GN=CHX13 PE=1 SV=1)

HSP 1 Score: 362.8 bits (930), Expect = 9.8e-99
Identity = 245/771 (31.78%), Postives = 406/771 (52.66%), Query Frame = 0

Query: 50  IACRVSRPSTSGDFFYGSNPLEDSFSALLLDVVFVILIIHLLHLFLRPLHQPKIVSQILG 109
           I C+     TS   F  SNPL+ +   LLL +  +I+   L+   L+PL Q  I +Q+L 
Sbjct: 28  IVCQAQNMLTSRGIFMKSNPLKYALPLLLLQMSVIIVTSRLIFRVLQPLKQGMISAQVLT 87

Query: 110 GFIIGPSVLGHNNNFRLHLFPEDVSFLLTNIGLIGFMYFLFISGVKTDLSLIKKAGKKEY 169
           G ++GPS LGHN  +     P     ++  +  +GF+  LF+ G+K D S+I+KAG K  
Sbjct: 88  GVVLGPSFLGHNVIYMNMFLPAGGKIIIQTLSNVGFVIHLFLLGLKIDGSIIRKAGSKAI 147

Query: 170 SIASFSVVVPLLLNISFALLIRKSMNEDLAKFSSIGAITSSLAITAFPVVHPILHELNLL 229
            I + S   P  L     + I K+M       S   +  S  ++T+FPV   +L ELN+L
Sbjct: 148 LIGTASYAFPFSLGNLTIMFISKTMGLPSDVISCTSSAISLSSMTSFPVTTTVLAELNIL 207

Query: 230 SSEVGRMSMSISIISDAVGINAVIAFEAAIQGETDAINSLWYLISLIVLLGFIVFGVRRA 289
           +SE+GR++   S++ +       +AF    +  T  + SL+ L  +I LL  I F  R  
Sbjct: 208 NSELGRLATHCSMVCEVCSWFVALAFNLYTRDRT--MTSLYALSMIIGLLLVIYFVFRPI 267

Query: 290 MHWIVKR---TPEGQAVEQGFVVAILLGVLIMGFLTDLFGIAILNGPLWLGMAIPDGPPL 349
           + W+ +R   + + + V   F V +LL +  +    +  G+    G  WLG+++PDGPPL
Sbjct: 268 IVWLTQRKTKSMDKKDVVPFFPVLLLLSIASLS--GEAMGVHAAFGAFWLGVSLPDGPPL 327

Query: 350 GSTLVERSETIISELLMPVSFAFVGLYTDVFEMAKAGWPTLAPLFFLALAGHFFKLGATL 409
           G+ L  + E   S L +P   A  GL T+ FE+ ++    +  +  + L  +  K   T 
Sbjct: 328 GTELAAKLEMFASNLFLPCFIAISGLQTNFFEITESHEHHVVMIEIILLITYGCKFLGTA 387

Query: 410 VASLYFQLPVRDSLAVSFIMCLRGQVEIVLLLHWMDKKIIKIPEFTMLVLMTATVTAIVT 469
            AS Y Q  + D+L ++F+MC +G +E+   + W D +++    F ++++    VT I  
Sbjct: 388 AASAYCQTQIGDALCLAFLMCCQGIIEVYTTIVWKDAQVVDTECFNLVIITILFVTGISR 447

Query: 470 PLVSILYDPTKPYMVNKRRTI----QHLPPGTKMKIVVCIEDQGDVAALVSLLDMSNPTV 529
            LV  LYDP+K Y    +RTI    QH     ++++++ + +  +V ++V+LL+ + PT 
Sbjct: 448 FLVVYLYDPSKRYKSKSKRTILNTRQH---NLQLRLLLGLYNVENVPSMVNLLEATYPTR 507

Query: 530 VSPFSIYALHLIELVGRAAPVFIDHKKSKAPSKYTA-SDSIHNALKLYEEARSELVKLHT 589
            +P S + LHL+EL GRA  +   H +       TA S  I NA + +E+     +    
Sbjct: 508 FNPISFFTLHLVELKGRAHALLTPHHQMNKLDPNTAQSTHIVNAFQRFEQKYQGALMAQH 567

Query: 590 YTAVAPKRTMNQDICELGLIKRANLIILPFSRRN------GEHSGVRLQNMNISVLEHAP 649
           +TA AP  ++N DIC L L K+A LI++PF ++       G+ +G  ++ +N++VL+ AP
Sbjct: 568 FTAAAPYSSINNDICTLALDKKATLIVIPFHKQYAIDGTVGQVNG-PIRTINLNVLDAAP 627

Query: 650 CSVGILVDKCNLHSPMVGQSFWNSAQHFVVLFLGGADAREALAYADRVIGNQDVYVSVIR 709
           CSV I +D+             N+ Q+  +LF+GG D  EALA   R+    D+ V++I 
Sbjct: 628 CSVAIFIDRGETEGRR-SVLMTNTWQNVAMLFIGGKDDAEALALCMRMAEKPDLNVTMIH 687

Query: 710 FLSQNSKGDNEFEKKLDDGMVTWFWVKNETNERVIYREVVVRNGAETIAAIQTMNDDSYD 769
           F  +++  D ++    +  +++ F        ++ Y E +VR+G ET   I ++  D+YD
Sbjct: 688 FRHKSALQDEDYSDMSEYNLISDFKSYAANKGKIHYVEEIVRDGVETTQVISSLG-DAYD 747

Query: 770 LVVVGRKQGINPVLLEGLSNWSNQNELGIIGDFVASEDFTAASSILVMQQQ 807
           +V+VGR   +   +L GL++WS   ELG+IGD + S DF    S+LV+ QQ
Sbjct: 748 MVLVGRDHDLESSVLYGLTDWSECPELGVIGDMLTSPDF--HFSVLVVHQQ 786

BLAST of MELO3C020516.2 vs. TrEMBL
Match: tr|A0A0A0M0W8|A0A0A0M0W8_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G533480 PE=4 SV=1)

HSP 1 Score: 1481.5 bits (3834), Expect = 0.0e+00
Identity = 758/830 (91.33%), Postives = 797/830 (96.02%), Query Frame = 0

Query: 1   MVRVFPVVGGNFAQYVWRNFH--GGDIISGKMVERIQVVGGVGHKTA-DGVGIACRVSRP 60
           MVRV PVVGGNFAQYVWRNFH  GGDII+GKM E IQVVGGVG KT  DG  +ACR+S P
Sbjct: 1   MVRVIPVVGGNFAQYVWRNFHGGGGDIITGKMAEGIQVVGGVGEKTGLDGPSMACRISHP 60

Query: 61  STSGDFFYGSNPLEDSFSALLLDVVFVILIIHLLHLFLRPLHQPKIVSQILGGFIIGPSV 120
            T G  FYGSNPLEDSFSALLLD+V VI IIHLLH  LRPLHQPKIVSQILGGFIIGPSV
Sbjct: 61  KTPG-VFYGSNPLEDSFSALLLDLVIVIFIIHLLHFLLRPLHQPKIVSQILGGFIIGPSV 120

Query: 121 LGHNNNFRLHLFPEDVSFLLTNIGLIGFMYFLFISGVKTDLSLIKKAGKKEYSIASFSVV 180
           LGHN NFRL+LFPEDVSFL+TNIGLIGFMYFLFISGVKTDLSLIKKAGKKEY IASFSVV
Sbjct: 121 LGHNKNFRLNLFPEDVSFLVTNIGLIGFMYFLFISGVKTDLSLIKKAGKKEYFIASFSVV 180

Query: 181 VPLLLNISFALLIRKSMNEDLAKFSSIGAITSSLAITAFPVVHPILHELNLLSSEVGRMS 240
           VPL+LNISFALLIRKSM+E+LAKFSSIGA+TSSLAITAFPVVHPILHELNLLSSEVGRMS
Sbjct: 181 VPLVLNISFALLIRKSMDENLAKFSSIGAVTSSLAITAFPVVHPILHELNLLSSEVGRMS 240

Query: 241 MSISIISDAVGINAVIAFEAAIQGETDAINSLWYLISLIVLLGFIVFGVRRAMHWIVKRT 300
           MSISIISDAVGINAVIAFEAAIQGETDA+N+LWYLISLI+LLGFIVFGVR+ MHWI+KRT
Sbjct: 241 MSISIISDAVGINAVIAFEAAIQGETDAMNALWYLISLIILLGFIVFGVRKVMHWIIKRT 300

Query: 301 PEGQAVEQGFVVAILLGVLIMGFLTDLFGIAILNGPLWLGMAIPDGPPLGSTLVERSETI 360
           PEGQAVEQGF++AILLGVL MGFLTDLFGIAILNGPLWLGMAIPDGPPLGSTLVERSETI
Sbjct: 301 PEGQAVEQGFIIAILLGVLTMGFLTDLFGIAILNGPLWLGMAIPDGPPLGSTLVERSETI 360

Query: 361 ISELLMPVSFAFVGLYTDVFEMAKAGWPTLAPLFFLALAGHFFKLGATLVASLYFQLPVR 420
           ISELLMPVSFAFVGLYTDVFEMAKAGWPTLAPLFFLALAGHFFKLGATL+ SL+FQLP+R
Sbjct: 361 ISELLMPVSFAFVGLYTDVFEMAKAGWPTLAPLFFLALAGHFFKLGATLIPSLFFQLPLR 420

Query: 421 DSLAVSFIMCLRGQVEIVLLLHWMDKKIIKIPEFTMLVLMTATVTAIVTPLVSILYDPTK 480
           DSLAVSFIMCLRGQVEI+LLLHW+DKKIIKIPEFTMLVLMTATVTAI+TPL+SILYDPTK
Sbjct: 421 DSLAVSFIMCLRGQVEIILLLHWIDKKIIKIPEFTMLVLMTATVTAILTPLISILYDPTK 480

Query: 481 PYMVNKRRTIQHLPPGTKMKIVVCIEDQGDVAALVSLLDMSNPTVVSPFSIYALHLIELV 540
           PYMV+KRRTIQHLPP TKMKIVVCIEDQ DVAALVSLLDMSNPT  SPFSIYALHLIELV
Sbjct: 481 PYMVSKRRTIQHLPPQTKMKIVVCIEDQEDVAALVSLLDMSNPTAASPFSIYALHLIELV 540

Query: 541 GRAAPVFIDHKKSKAPSKYTASDSIHNALKLYEEARSELVKLHTYTAVAPKRTMNQDICE 600
           GRAAPVFIDHKKSKAPSKYTASDSIHNALKLYEEARSELVKLHTYTAVAPKRTMNQDICE
Sbjct: 541 GRAAPVFIDHKKSKAPSKYTASDSIHNALKLYEEARSELVKLHTYTAVAPKRTMNQDICE 600

Query: 601 LGLIKRANLIILPFSRRNGEHSGVRLQNMNISVLEHAPCSVGILVDKCNLHSPMVGQSFW 660
           LGLIKRANLI LPFS RNG+HSGVRLQ+MN SVLEHAPCSVGILVDKCNLHSPMVGQ+FW
Sbjct: 601 LGLIKRANLIFLPFS-RNGQHSGVRLQDMNTSVLEHAPCSVGILVDKCNLHSPMVGQAFW 660

Query: 661 NSAQHFVVLFLGGADAREALAYADRVIGNQDVYVSVIRFLSQNSKGDNEFEKKLDDGMVT 720
           NSAQH VVLFLGGADAREALAYADRVIGNQDVYVSVIRFL+QNS+GDNEFEKKLDDGMVT
Sbjct: 661 NSAQHIVVLFLGGADAREALAYADRVIGNQDVYVSVIRFLAQNSRGDNEFEKKLDDGMVT 720

Query: 721 WFWVKNETNERVIYREVVVRNGAETIAAIQTMNDDSYDLVVVGRKQGINPVLLEGLSNWS 780
           WFWVKNETNERVIYREVVVRNGAETI AIQ++NDDSYDLV+VGRKQGINPVLLEGLSNWS
Sbjct: 721 WFWVKNETNERVIYREVVVRNGAETITAIQSLNDDSYDLVIVGRKQGINPVLLEGLSNWS 780

Query: 781 NQNELGIIGDFVASEDFTAASSILVMQQQVLRDQGQFSSGVCGKIRFDIR 828
           +QNELGI+GDFVASEDFTAASS+LV+QQQ+LRDQGQFSSG+CGKIRFDIR
Sbjct: 781 HQNELGIVGDFVASEDFTAASSVLVLQQQILRDQGQFSSGICGKIRFDIR 828

BLAST of MELO3C020516.2 vs. TrEMBL
Match: tr|A0A1S3C5D1|A0A1S3C5D1_CUCME (cation/H(+) antiporter 24 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103497047 PE=4 SV=1)

HSP 1 Score: 1473.8 bits (3814), Expect = 0.0e+00
Identity = 764/764 (100.00%), Postives = 764/764 (100.00%), Query Frame = 0

Query: 64  FYGSNPLEDSFSALLLDVVFVILIIHLLHLFLRPLHQPKIVSQILGGFIIGPSVLGHNNN 123
           FYGSNPLEDSFSALLLDVVFVILIIHLLHLFLRPLHQPKIVSQILGGFIIGPSVLGHNNN
Sbjct: 12  FYGSNPLEDSFSALLLDVVFVILIIHLLHLFLRPLHQPKIVSQILGGFIIGPSVLGHNNN 71

Query: 124 FRLHLFPEDVSFLLTNIGLIGFMYFLFISGVKTDLSLIKKAGKKEYSIASFSVVVPLLLN 183
           FRLHLFPEDVSFLLTNIGLIGFMYFLFISGVKTDLSLIKKAGKKEYSIASFSVVVPLLLN
Sbjct: 72  FRLHLFPEDVSFLLTNIGLIGFMYFLFISGVKTDLSLIKKAGKKEYSIASFSVVVPLLLN 131

Query: 184 ISFALLIRKSMNEDLAKFSSIGAITSSLAITAFPVVHPILHELNLLSSEVGRMSMSISII 243
           ISFALLIRKSMNEDLAKFSSIGAITSSLAITAFPVVHPILHELNLLSSEVGRMSMSISII
Sbjct: 132 ISFALLIRKSMNEDLAKFSSIGAITSSLAITAFPVVHPILHELNLLSSEVGRMSMSISII 191

Query: 244 SDAVGINAVIAFEAAIQGETDAINSLWYLISLIVLLGFIVFGVRRAMHWIVKRTPEGQAV 303
           SDAVGINAVIAFEAAIQGETDAINSLWYLISLIVLLGFIVFGVRRAMHWIVKRTPEGQAV
Sbjct: 192 SDAVGINAVIAFEAAIQGETDAINSLWYLISLIVLLGFIVFGVRRAMHWIVKRTPEGQAV 251

Query: 304 EQGFVVAILLGVLIMGFLTDLFGIAILNGPLWLGMAIPDGPPLGSTLVERSETIISELLM 363
           EQGFVVAILLGVLIMGFLTDLFGIAILNGPLWLGMAIPDGPPLGSTLVERSETIISELLM
Sbjct: 252 EQGFVVAILLGVLIMGFLTDLFGIAILNGPLWLGMAIPDGPPLGSTLVERSETIISELLM 311

Query: 364 PVSFAFVGLYTDVFEMAKAGWPTLAPLFFLALAGHFFKLGATLVASLYFQLPVRDSLAVS 423
           PVSFAFVGLYTDVFEMAKAGWPTLAPLFFLALAGHFFKLGATLVASLYFQLPVRDSLAVS
Sbjct: 312 PVSFAFVGLYTDVFEMAKAGWPTLAPLFFLALAGHFFKLGATLVASLYFQLPVRDSLAVS 371

Query: 424 FIMCLRGQVEIVLLLHWMDKKIIKIPEFTMLVLMTATVTAIVTPLVSILYDPTKPYMVNK 483
           FIMCLRGQVEIVLLLHWMDKKIIKIPEFTMLVLMTATVTAIVTPLVSILYDPTKPYMVNK
Sbjct: 372 FIMCLRGQVEIVLLLHWMDKKIIKIPEFTMLVLMTATVTAIVTPLVSILYDPTKPYMVNK 431

Query: 484 RRTIQHLPPGTKMKIVVCIEDQGDVAALVSLLDMSNPTVVSPFSIYALHLIELVGRAAPV 543
           RRTIQHLPPGTKMKIVVCIEDQGDVAALVSLLDMSNPTVVSPFSIYALHLIELVGRAAPV
Sbjct: 432 RRTIQHLPPGTKMKIVVCIEDQGDVAALVSLLDMSNPTVVSPFSIYALHLIELVGRAAPV 491

Query: 544 FIDHKKSKAPSKYTASDSIHNALKLYEEARSELVKLHTYTAVAPKRTMNQDICELGLIKR 603
           FIDHKKSKAPSKYTASDSIHNALKLYEEARSELVKLHTYTAVAPKRTMNQDICELGLIKR
Sbjct: 492 FIDHKKSKAPSKYTASDSIHNALKLYEEARSELVKLHTYTAVAPKRTMNQDICELGLIKR 551

Query: 604 ANLIILPFSRRNGEHSGVRLQNMNISVLEHAPCSVGILVDKCNLHSPMVGQSFWNSAQHF 663
           ANLIILPFSRRNGEHSGVRLQNMNISVLEHAPCSVGILVDKCNLHSPMVGQSFWNSAQHF
Sbjct: 552 ANLIILPFSRRNGEHSGVRLQNMNISVLEHAPCSVGILVDKCNLHSPMVGQSFWNSAQHF 611

Query: 664 VVLFLGGADAREALAYADRVIGNQDVYVSVIRFLSQNSKGDNEFEKKLDDGMVTWFWVKN 723
           VVLFLGGADAREALAYADRVIGNQDVYVSVIRFLSQNSKGDNEFEKKLDDGMVTWFWVKN
Sbjct: 612 VVLFLGGADAREALAYADRVIGNQDVYVSVIRFLSQNSKGDNEFEKKLDDGMVTWFWVKN 671

Query: 724 ETNERVIYREVVVRNGAETIAAIQTMNDDSYDLVVVGRKQGINPVLLEGLSNWSNQNELG 783
           ETNERVIYREVVVRNGAETIAAIQTMNDDSYDLVVVGRKQGINPVLLEGLSNWSNQNELG
Sbjct: 672 ETNERVIYREVVVRNGAETIAAIQTMNDDSYDLVVVGRKQGINPVLLEGLSNWSNQNELG 731

Query: 784 IIGDFVASEDFTAASSILVMQQQVLRDQGQFSSGVCGKIRFDIR 828
           IIGDFVASEDFTAASSILVMQQQVLRDQGQFSSGVCGKIRFDIR
Sbjct: 732 IIGDFVASEDFTAASSILVMQQQVLRDQGQFSSGVCGKIRFDIR 775

BLAST of MELO3C020516.2 vs. TrEMBL
Match: tr|A0A1S3C589|A0A1S3C589_CUCME (cation/H(+) antiporter 24 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103497047 PE=4 SV=1)

HSP 1 Score: 1467.2 bits (3797), Expect = 0.0e+00
Identity = 763/764 (99.87%), Postives = 763/764 (99.87%), Query Frame = 0

Query: 64  FYGSNPLEDSFSALLLDVVFVILIIHLLHLFLRPLHQPKIVSQILGGFIIGPSVLGHNNN 123
           FYGSNPLEDSFSALLLDVVFVILIIHLLHLFLRPLHQPKIVSQILGGFIIGPSVLGHNNN
Sbjct: 12  FYGSNPLEDSFSALLLDVVFVILIIHLLHLFLRPLHQPKIVSQILGGFIIGPSVLGHNNN 71

Query: 124 FRLHLFPEDVSFLLTNIGLIGFMYFLFISGVKTDLSLIKKAGKKEYSIASFSVVVPLLLN 183
           FRLHLFPEDVSFLLTNIGLIGFMYFLFISGVKTDLSLIKKAGKKEYSIASFSVVVPLLLN
Sbjct: 72  FRLHLFPEDVSFLLTNIGLIGFMYFLFISGVKTDLSLIKKAGKKEYSIASFSVVVPLLLN 131

Query: 184 ISFALLIRKSMNEDLAKFSSIGAITSSLAITAFPVVHPILHELNLLSSEVGRMSMSISII 243
           ISFALLIRKSMNEDLAKFSSIGAITSSLAITAFPVVHPILHELNLLSSEVGRMSMSISII
Sbjct: 132 ISFALLIRKSMNEDLAKFSSIGAITSSLAITAFPVVHPILHELNLLSSEVGRMSMSISII 191

Query: 244 SDAVGINAVIAFEAAIQGETDAINSLWYLISLIVLLGFIVFGVRRAMHWIVKRTPEGQAV 303
           SDAVGINAVIAFEAAIQGETDAINSLWYLISLIVLLGFIVFGVRRAMHWIVKRTPEGQAV
Sbjct: 192 SDAVGINAVIAFEAAIQGETDAINSLWYLISLIVLLGFIVFGVRRAMHWIVKRTPEGQAV 251

Query: 304 EQGFVVAILLGVLIMGFLTDLFGIAILNGPLWLGMAIPDGPPLGSTLVERSETIISELLM 363
           EQGFVVAILLGVLIMGFLTDLFGIAILNGPLWLGMAIPDGPPLGSTLVERSETIISELLM
Sbjct: 252 EQGFVVAILLGVLIMGFLTDLFGIAILNGPLWLGMAIPDGPPLGSTLVERSETIISELLM 311

Query: 364 PVSFAFVGLYTDVFEMAKAGWPTLAPLFFLALAGHFFKLGATLVASLYFQLPVRDSLAVS 423
           PVSFAFVGLYTDVFEMAKAGWPTLAPLFFLALAGHFFKLGATLVASLYFQLPVRDSLAVS
Sbjct: 312 PVSFAFVGLYTDVFEMAKAGWPTLAPLFFLALAGHFFKLGATLVASLYFQLPVRDSLAVS 371

Query: 424 FIMCLRGQVEIVLLLHWMDKKIIKIPEFTMLVLMTATVTAIVTPLVSILYDPTKPYMVNK 483
           FIMCLRGQVEIVLLLHWMDKKIIKIPEFTMLVLMTATVTAIVTPLVSILYDPTKPYMVNK
Sbjct: 372 FIMCLRGQVEIVLLLHWMDKKIIKIPEFTMLVLMTATVTAIVTPLVSILYDPTKPYMVNK 431

Query: 484 RRTIQHLPPGTKMKIVVCIEDQGDVAALVSLLDMSNPTVVSPFSIYALHLIELVGRAAPV 543
           RRTIQHLPPGTKMKIVVCIEDQGDVAALVSLLDMSNPTVVSPFSIYALHLIELVGRAAPV
Sbjct: 432 RRTIQHLPPGTKMKIVVCIEDQGDVAALVSLLDMSNPTVVSPFSIYALHLIELVGRAAPV 491

Query: 544 FIDHKKSKAPSKYTASDSIHNALKLYEEARSELVKLHTYTAVAPKRTMNQDICELGLIKR 603
           FIDHKKSKAPSKYTASDSIHNALKLYEEARSELVKLHTYTAVAPKRTMNQDICELGLIKR
Sbjct: 492 FIDHKKSKAPSKYTASDSIHNALKLYEEARSELVKLHTYTAVAPKRTMNQDICELGLIKR 551

Query: 604 ANLIILPFSRRNGEHSGVRLQNMNISVLEHAPCSVGILVDKCNLHSPMVGQSFWNSAQHF 663
           ANLIILPFS RNGEHSGVRLQNMNISVLEHAPCSVGILVDKCNLHSPMVGQSFWNSAQHF
Sbjct: 552 ANLIILPFS-RNGEHSGVRLQNMNISVLEHAPCSVGILVDKCNLHSPMVGQSFWNSAQHF 611

Query: 664 VVLFLGGADAREALAYADRVIGNQDVYVSVIRFLSQNSKGDNEFEKKLDDGMVTWFWVKN 723
           VVLFLGGADAREALAYADRVIGNQDVYVSVIRFLSQNSKGDNEFEKKLDDGMVTWFWVKN
Sbjct: 612 VVLFLGGADAREALAYADRVIGNQDVYVSVIRFLSQNSKGDNEFEKKLDDGMVTWFWVKN 671

Query: 724 ETNERVIYREVVVRNGAETIAAIQTMNDDSYDLVVVGRKQGINPVLLEGLSNWSNQNELG 783
           ETNERVIYREVVVRNGAETIAAIQTMNDDSYDLVVVGRKQGINPVLLEGLSNWSNQNELG
Sbjct: 672 ETNERVIYREVVVRNGAETIAAIQTMNDDSYDLVVVGRKQGINPVLLEGLSNWSNQNELG 731

Query: 784 IIGDFVASEDFTAASSILVMQQQVLRDQGQFSSGVCGKIRFDIR 828
           IIGDFVASEDFTAASSILVMQQQVLRDQGQFSSGVCGKIRFDIR
Sbjct: 732 IIGDFVASEDFTAASSILVMQQQVLRDQGQFSSGVCGKIRFDIR 774

BLAST of MELO3C020516.2 vs. TrEMBL
Match: tr|A0A1Q3ASP2|A0A1Q3ASP2_CEPFO (Na_H_Exchanger domain-containing protein OS=Cephalotus follicularis OX=3775 GN=CFOL_v3_02203 PE=4 SV=1)

HSP 1 Score: 902.9 bits (2332), Expect = 5.3e-259
Identity = 451/781 (57.75%), Postives = 598/781 (76.57%), Query Frame = 0

Query: 42  HKTADGVGI---ACRVSRPSTSGDFFYGSNPLEDSFSALLLDVVFVILIIHLLHLFLRPL 101
           H  A G  +    CR          FY  + L+ +F+ +LL+++FVILI  ++   L+PL
Sbjct: 28  HVDAGGASLFPSICRKLHSPLPFGIFYRHDNLDHTFAVILLEIIFVILITRIIRFILKPL 87

Query: 102 HQPKIVSQILGGFIIGPSVLGHNNNFRLHLFPEDVSFLLTNIGLIGFMYFLFISGVKTDL 161
            QP+IVS+ILGG  IGPS+LG N  F   +  ++ +F++ N+G++GFM F+FI+GVK D+
Sbjct: 88  KQPRIVSEILGGIFIGPSLLGQNKKFAQRVLQDNATFVVRNLGIMGFMLFVFITGVKMDI 147

Query: 162 SLIKKAGKKEYSIASFSVVVPLLLNISFALLIRKSMNEDLAKFSSIGAITSSLAITAFPV 221
           SL+KK+GKK Y  A   V  P++ +    LL+R SM++DL++ SSIGA+ S +A+ AF V
Sbjct: 148 SLVKKSGKKHYYTALIGVAFPMVTSSVVGLLLRNSMDKDLSRLSSIGAVASDVALPAFHV 207

Query: 222 VHPILHELNLLSSEVGRMSMSISIISDAVGINAVIAFEAAIQGETDAINSLWYLISLIVL 281
           ++PIL ELNLLSSEVGRM++S SIISDAVG++A++AFEAA QGE  A ++LWYL S ++L
Sbjct: 208 IYPILKELNLLSSEVGRMALSTSIISDAVGVSAILAFEAAKQGEGTASDALWYLCSTLIL 267

Query: 282 LGFIVFGVRRAMHWIVKRTPEGQAVEQGFVVAILLGVLIMGFLTDLFGIAILNGPLWLGM 341
             F+V  V+R M WI+ +TPEG+ V+Q +VVAILLGV +MGFLTD+FGIAI+NGPLWLG+
Sbjct: 268 GTFVVTVVKRGMTWIIDKTPEGKPVDQSYVVAILLGVFVMGFLTDMFGIAIINGPLWLGL 327

Query: 342 AIPDGPPLGSTLVERSETIISELLMPVSFAFVGLYTDVFEMAKAGWPTLAPLFFLALAGH 401
            I DGPPLGSTLV++SETII +++MP ++A +GLYTDV+ M+  GW TLAPLF +A++G+
Sbjct: 328 IILDGPPLGSTLVDKSETIIMDVIMPFTYAMLGLYTDVYSMSALGWSTLAPLFAMAVSGY 387

Query: 402 FFKLGATLVASLYFQLPVRDSLAVSFIMCLRGQVEIVLLLHWMDKKIIKIPEFTMLVLMT 461
             KL  T V +  F+ P RD L +S IM LRGQ+E +++LHW+DK+II IP FT++VL+T
Sbjct: 388 VSKLIGTFVTAAIFETPFRDCLTLSLIMSLRGQMEFIIILHWLDKRIISIPGFTLMVLLT 447

Query: 462 ATVTAIVTPLVSILYDPTKPYMVNKRRTIQHLPPGTKMKIVVCIEDQGDVAALVSLLDMS 521
             +TAI TPL+S LYDPT+PYMV KRRT+QH PP TK++IV+C  DQ  +A L++LL++S
Sbjct: 448 TAITAICTPLISTLYDPTRPYMVTKRRTLQHTPPNTKLRIVLCFHDQQSIAGLINLLEVS 507

Query: 522 NPTVVSPFSIYALHLIELVGRAAPVFIDHKKSKAPSKYTASDSIHNALKLYEEARSELVK 581
           NPT+ +PF +Y L LIEL+GRAAPVF+DHKK +  S+Y   D+I NAL+LYEE R E VK
Sbjct: 508 NPTLTNPFKVYVLRLIELIGRAAPVFVDHKKEEENSRYRMQDTIQNALQLYEETRGECVK 567

Query: 582 LHTYTAVAPKRTMNQDICELGLIKRANLIILPFSRRNGEHSGVR------LQNMNISVLE 641
           +H +TAVAPKRTM QDICEL L+ +A LIILPFS    E  GV       +Q +N  VL 
Sbjct: 568 IHAFTAVAPKRTMYQDICELALLNKAALIILPFSE---EVHGVTEPALQGMQTVNSDVLT 627

Query: 642 HAPCSVGILVDKCN-LHSPMVGQSFWNSAQHFVVLFLGGADAREALAYADRVIGNQDVYV 701
           HAPCSVG+LVDK +   +P+VG SF  +  +F++LFLGGAD+REALA+ADR++GNQDV +
Sbjct: 628 HAPCSVGVLVDKSSGYRNPIVGDSFRGTFHNFMLLFLGGADSREALAFADRMVGNQDVSL 687

Query: 702 SVIRFLSQNSKGDNEFEKKLDDGMVTWFWVKNETNERVIYREVVVRNGAETIAAIQTMND 761
           +V+RFLS N  GD+E EKKLDDG+VTWFWVKNE NERVIYREVVV++G ET++AIQ +ND
Sbjct: 688 TVLRFLSHNYVGDDEMEKKLDDGVVTWFWVKNEANERVIYREVVVKSGEETVSAIQAVND 747

Query: 762 DSYDLVVVGRKQGINPVLLEGLSNWSNQNELGIIGDFVASEDFTAASSILVMQQQVLRDQ 813
           DSYDL + GRK+GIN VLLEGL NWS + ELGIIGD+VAS DF++ +S+LV+QQQVLR  
Sbjct: 748 DSYDLWITGRKEGINQVLLEGLENWSEKLELGIIGDYVASGDFSSTASVLVIQQQVLRGH 805

BLAST of MELO3C020516.2 vs. TrEMBL
Match: tr|A0A2P4ICV4|A0A2P4ICV4_QUESU (Cation/h(+) antiporter 24 OS=Quercus suber OX=58331 GN=CFP56_15066 PE=4 SV=1)

HSP 1 Score: 893.6 bits (2308), Expect = 3.2e-256
Identity = 453/765 (59.22%), Postives = 586/765 (76.60%), Query Frame = 0

Query: 50  IACRVSRPSTSGDFFYGSNPLEDSFSALLLDVVFVILIIHLLHLFLRPLHQPKIVSQILG 109
           + C   + +     FYG NPL  SF+ LLL++  VI+II  L   L+PL QP++V++I+G
Sbjct: 45  LTCHSIQSTHPYGIFYGENPLNSSFTLLLLEITLVIVIIQTLRFLLKPLKQPRVVAEIIG 104

Query: 110 GFIIGPSVLGHNNNFRLHLFPEDVSFLLTNIGLIGFMYFLFISGVKTDLSLIKKAGKKEY 169
           G IIGPSVLGHN  F   +FP++  ++L NIGL+GFMYF+F+ GVK DLSL+KKAGKKE 
Sbjct: 105 GIIIGPSVLGHNKKFFSCMFPDNTQYVLRNIGLMGFMYFVFVIGVKMDLSLVKKAGKKEM 164

Query: 170 SIASFSVVVPLLLNISFALLIRKSMNEDLAKFSSIGAITSSLAITAFPVVHPILHELNLL 229
            IA   +V P ++ +  A+++R SM++DLAKF+SIGAI+SS+AIT+FP ++ IL ELNLL
Sbjct: 165 YIAFAGMVFPFIIVVLAAMIMRPSMDKDLAKFTSIGAISSSMAITSFPCIYTILEELNLL 224

Query: 230 SSEVGRMSMSISIISDAVGINAVIAFEAAIQGETDAINSLWYLISLIVLLGFIVFGVRRA 289
           SSEVGRMS+S SII DA+G+NA  AFEAA QGET  + +LWYLISL VL+ F V  V R 
Sbjct: 225 SSEVGRMSLSTSIICDAIGLNAFYAFEAAKQGETKPMAALWYLISLAVLVAFCVIFVGRG 284

Query: 290 MHWIVKRTPEGQAVEQGFVVAILLGVLIMGFLTDLFGIAILNGPLWLGMAIPDGPPLGST 349
           M WI   TPEG  V+QG+VVAILL VL+MGFLTD FG+AI  GP+ LG+ IPDGPPLGST
Sbjct: 285 MQWIADHTPEGDPVDQGYVVAILLLVLVMGFLTDFFGVAIATGPMMLGLVIPDGPPLGST 344

Query: 350 LVERSETIISELLMPVSFAFVGLYTDVFEMAKAGWPTLAPLFFLALAGHFFKLGATLVAS 409
           LV R ET+I E LMP++F F+G+ TDV+ M   GW  L+PLF LAL G+F KL  T +A+
Sbjct: 345 LVARCETLIFEFLMPIAFIFIGITTDVYSMTAVGWVHLSPLFALALIGYFSKLLVTAMAA 404

Query: 410 LYFQLPVRDSLAVSFIMCLRGQVEIVLLLHWMDKKIIKIPEFTMLVLMTATVTAIVTPLV 469
           ++  +P+R+S A+SFI+ LRGQVEI+L LHWMDK II  P F++L+L+TA VT   T L+
Sbjct: 405 VFVNVPLRESFAMSFILSLRGQVEILLYLHWMDKVIIDQPGFSLLILLTAAVTGTCTALI 464

Query: 470 SILYDPTKPYMVNKRRTIQHLPPGTKMKIVVCIEDQGDVAALVSLLDMSNPTVVSPFSIY 529
           SI YDPTKPYMVNKRRTIQH  P  +++IV+C+ D   V  L+ L+++SNPTV SPFSI+
Sbjct: 465 SIFYDPTKPYMVNKRRTIQHTSPENEVRIVLCMHDLESVQGLIRLVEVSNPTVTSPFSIF 524

Query: 530 ALHLIELVGRAAPVFIDHKKSKAPSKYTASDSIHNALKLYEEARSELVKLHTYTAVAPKR 589
           ALHLIELVGRAAP+FIDH++ K PSKY +S++I  ALKLY+E+++  + L T+TAV+PK+
Sbjct: 525 ALHLIELVGRAAPLFIDHEEQKLPSKYGSSEAILGALKLYQESQNAYLNLRTFTAVSPKK 584

Query: 590 TMNQDICELGLIKRANLIILPFSRRNGEHSGVR-----LQNMNISVLEHAPCSVGILVDK 649
           TM QDIC+L L  +A L+ILPF + + +  G       ++++N +VL HAPCSVGILVDK
Sbjct: 585 TMFQDICKLALECKATLVILPFHKEHLDIGGAEVVLAGVRSVNYNVLAHAPCSVGILVDK 644

Query: 650 CNLHSPMVGQSFWNSAQHFVVLFLGGADAREALAYADRVIGNQDVYVSVIRFLSQNSKGD 709
            N    +      NSA HFV+LFLGGADAREAL +ADR+ GN+ + ++VIRFLS N  GD
Sbjct: 645 GNFQRAVPAIYTENSALHFVLLFLGGADAREALVFADRMAGNRSISLTVIRFLSYNFNGD 704

Query: 710 NEFEKKLDDGMVTWFWVKNETNERVIYREVVVRNGAETIAAIQTMNDDSYDLVVVGRKQG 769
           NE EKKLDDG+VTWFWVKNE N+RV+YREVVVR+G ETIAAI+ MND+SYDL +VGRK G
Sbjct: 705 NEMEKKLDDGLVTWFWVKNEKNDRVVYREVVVRDGDETIAAIEAMNDESYDLWIVGRKHG 764

Query: 770 INPVLLEGLSNWSNQNELGIIGDFVASEDFTAASSILVMQQQVLR 810
           INPVLLEGL+ W+   ELG+IGD++ S DF +++S+LVMQQQ++R
Sbjct: 765 INPVLLEGLTKWNKNQELGVIGDYLTSVDFGSSASVLVMQQQIMR 809