MELO3C016793.2 (gene) Melon (DHL92) v3.6.1

BLAST of MELO3C016793.2 vs. NCBI nr
Match: XP_008453067.1 (PREDICTED: LOW QUALITY PROTEIN: cytochrome P450 81E8-like [Cucumis melo])

HSP 1 Score: 1036.6 bits (2679), Expect = 3.0e-299
Identity = 517/521 (99.23%), Postives = 518/521 (99.42%), Query Frame = 0

Query: 14  MTYPSMEPHTITWFSLSLLLITTLILLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKEPFH 73
           MTYPSMEPHTITWFSLSLLLITTLILLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKEPFH
Sbjct: 1   MTYPSMEPHTITWFSLSLLLITTLILLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKEPFH 60

Query: 74  RVLHNLSNKYGPILSLTIGSRPVLVVSSPTAVRECFTKNDIVFANRPRLISGKYINYNYT 133
           RVLHNLSNKYGPILSLTIGSRPVLVVSSPTAVRECFTKNDIVFANRPRLISGKYINYNYT
Sbjct: 61  RVLHNLSNKYGPILSLTIGSRPVLVVSSPTAVRECFTKNDIVFANRPRLISGKYINYNYT 120

Query: 134 AVGFAPYGQHWRNMRRIATTELLSNHRLNMYLNIRVEELKLWVKNNLYKWGSGGDFVVVD 193
           AVGFAPYGQHWRNMRRIATTELLSNHRLNMYLNIRVEELKLWVKNNLYKWGSGGDFVVVD
Sbjct: 121 AVGFAPYGQHWRNMRRIATTELLSNHRLNMYLNIRVEELKLWVKNNLYKWGSGGDFVVVD 180

Query: 194 MKSKLKELSFNTVMRMISGKRYFGVEVEDVEEAFEFREIMKELLELSGATNAADFLKILR 253
           MKSKLKELSFNTVMRMISGKRYFGVEVEDVEEAFEFREIMKELLELSGATNAADFLKILR
Sbjct: 181 MKSKLKELSFNTVMRMISGKRYFGVEVEDVEEAFEFREIMKELLELSGATNAADFLKILR 240

Query: 254 VFDLEGVKKRMMKASGRADVFLQKLIDEERKKKRVSRWPEEKQKQAKTSMIRTLLSLQES 313
           VFDLEGVKKRMMKASGRADVFLQKLIDEERKK+   RWPEEKQKQAKTSMIRTLLSLQES
Sbjct: 241 VFDLEGVKKRMMKASGRADVFLQKLIDEERKKE--FRWPEEKQKQAKTSMIRTLLSLQES 300

Query: 314 QPQYYSDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPMTMKKAWIEIRDCVGENQMV 373
           QPQYYSDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPMTMKKAWIEIRDCVGENQMV
Sbjct: 301 QPQYYSDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPMTMKKAWIEIRDCVGENQMV 360

Query: 374 EEGDVSELKYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFEVPKGTMLLVNAWGIHRD 433
           EEGDVSELKYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFEVPKGTMLLVNAWGIHRD
Sbjct: 361 EEGDVSELKYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFEVPKGTMLLVNAWGIHRD 420

Query: 434 PKVWEDPTSFQPERFLNWEGVETYKWIPFGMGRRACPGVALAQRSMGLTLATLIQCFEWK 493
           PKVWEDPTSFQPERFLNWEGVETYKWIPFGMGRRACPGVALAQRSMGLTLATLIQCFEWK
Sbjct: 421 PKVWEDPTSFQPERFLNWEGVETYKWIPFGMGRRACPGVALAQRSMGLTLATLIQCFEWK 480

Query: 494 KVDEQEQIDLSEGSGITMPKAKALEAMCKPRTSMLHLLAQL 535
           KVDEQEQIDLSEGSGITMPKAKALEAMCKPRTSMLHLLAQL
Sbjct: 481 KVDEQEQIDLSEGSGITMPKAKALEAMCKPRTSMLHLLAQL 519

BLAST of MELO3C016793.2 vs. NCBI nr
Match: XP_004141393.1 (PREDICTED: cytochrome P450 81E8-like [Cucumis sativus])

HSP 1 Score: 911.0 bits (2353), Expect = 1.9e-261
Identity = 455/517 (88.01%), Postives = 477/517 (92.26%), Query Frame = 0

Query: 19  MEPHTITWFSLSLLLITTLILLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKEPFHRVLHN 78
           MEPH +T  SLSLLLI+TLI                      IIGHLHLLK+PF+RVLH+
Sbjct: 1   MEPHALTCLSLSLLLISTLIXXXXXXXXXXXXXXXXXXXXXXIIGHLHLLKQPFYRVLHD 60

Query: 79  LSNKYGPILSLTIGSRPVLVVSSPTAVRECFTKNDIVFANRPRLISGKYINYNYTAVGFA 138
           LSNKYGPILSLTIGSRPV+VVSSPTAVRECFTKNDIVFANRPRL+SGKYINYNYTA+GFA
Sbjct: 61  LSNKYGPILSLTIGSRPVVVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAMGFA 120

Query: 139 PYGQHWRNMRRIATTELLSNHRLNMYLNIRVEELKLWVKNNLYKWGSG-GDFVVVDMKSK 198
           PYGQHWRNMRRIATTELLSNHRLN YLNIRVEELKLWVKN+LYKW SG GDFVVVDMK K
Sbjct: 121 PYGQHWRNMRRIATTELLSNHRLNTYLNIRVEELKLWVKNSLYKWSSGRGDFVVVDMKYK 180

Query: 199 LKELSFNTVMRMISGKRYFGVEVEDVEEAFEFREIMKELLELSGATNAADFLKILRVFDL 258
           LKELSFNTVMRMISGKRY+GVEVEDVEEA EFREIMKELLELSGATNAADF  ILRVFD+
Sbjct: 181 LKELSFNTVMRMISGKRYYGVEVEDVEEALEFREIMKELLELSGATNAADFFTILRVFDI 240

Query: 259 EGVKKRMMKASGRADVFLQKLIDEERKKKRVSRWPEEKQKQAKTSMIRTLLSLQESQPQY 318
           EGVKK+MMKASGRADVFLQKLIDEER +K VSRWPEEKQK+ KTSMIRTLLSLQESQPQY
Sbjct: 241 EGVKKKMMKASGRADVFLQKLIDEER-EKGVSRWPEEKQKEGKTSMIRTLLSLQESQPQY 300

Query: 319 YSDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPMTMKKAWIEIRDCVGENQMVEEGD 378
           YSDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPM M+KAWIEIRDC+GENQMVEEGD
Sbjct: 301 YSDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPMVMEKAWIEIRDCIGENQMVEEGD 360

Query: 379 VSELKYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFEVPKGTMLLVNAWGIHRDPKVW 438
           VS+LKYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFE+PKGTML+VNAW IHRDPKVW
Sbjct: 361 VSKLKYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFEIPKGTMLMVNAWAIHRDPKVW 420

Query: 439 EDPTSFQPERFLNWEGVETYKWIPFGMGRRACPGVALAQRSMGLTLATLIQCFEWKKVDE 498
           EDPTSF+PERFLNWEGVE+YKWIPFGMGRRACPG ALAQRSMGLTLATLIQCFEW+KVDE
Sbjct: 421 EDPTSFRPERFLNWEGVESYKWIPFGMGRRACPGFALAQRSMGLTLATLIQCFEWEKVDE 480

Query: 499 QEQIDLSEGSGITMPKAKALEAMCKPRTSMLHLLAQL 535
            EQIDLSEGSGITMPKAKALEAMCKPR SMLHLLAQL
Sbjct: 481 NEQIDLSEGSGITMPKAKALEAMCKPRNSMLHLLAQL 516

BLAST of MELO3C016793.2 vs. NCBI nr
Match: XP_022977005.1 (isoflavone 3'-hydroxylase-like [Cucurbita maxima])

HSP 1 Score: 774.6 bits (1999), Expect = 2.1e-220
Identity = 389/520 (74.81%), Postives = 451/520 (86.73%), Query Frame = 0

Query: 15  TYPSMEPHTITWFSLSLLLITTLILLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKEPFHR 74
           TY SME +TIT  SLSLLL  TLIL+ KLRK ++ +  PP+PPSLP+IGHLHLLK+PFHR
Sbjct: 8   TYSSMEANTITCLSLSLLL--TLILIYKLRK-QSPRNRPPSPPSLPLIGHLHLLKQPFHR 67

Query: 75  VLHNLSNKYGPILSLTIGSRPVLVVSSPTAVRECFTKNDIVFANRPRLISGKYINYNYTA 134
           VL +LSNK+GPILSL+IGSRP++V+SSPTAVRECFTKNDI  ANRPRL++GKY+NYNYT 
Sbjct: 68  VLQDLSNKHGPILSLSIGSRPLVVISSPTAVRECFTKNDIALANRPRLLAGKYMNYNYTG 127

Query: 135 VGFAPYGQHWRNMRRIATTELLSNHRLNMYLNIRVEELKLWVKNNLYKWGSGGDFVVVDM 194
           V FA YG HWRNMRRIATTELLSNHRLN YLNIRV+ELKL VKN     G   DFV ++M
Sbjct: 128 VAFASYGHHWRNMRRIATTELLSNHRLNAYLNIRVQELKL-VKNLYGATGESSDFVRLEM 187

Query: 195 KSKLKELSFNTVMRMISGKRYFGVEVEDVEEAFEFREIMKELLELSGATNAADFLKILRV 254
           +SKL ELSFN VMRM+SGKRYFGV+VEDVEEA EFREIMKELL+L GA+N ADFL ILR+
Sbjct: 188 QSKLNELSFNIVMRMVSGKRYFGVDVEDVEEAREFREIMKELLQLGGASNPADFLPILRL 247

Query: 255 FDLEGVKKRMMKASGRADVFLQKLIDEERKKKRVSRWPEEKQKQAKTSMIRTLLSLQESQ 314
           FD +G++KRM++ASGRAD FLQ LID ER  +R SRWPEE  K+   +MI++LLSLQESQ
Sbjct: 248 FDYQGLEKRMVQASGRADSFLQNLIDCER-DRRASRWPEE--KRGNKTMIQSLLSLQESQ 307

Query: 315 PQYYSDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPMTMKKAWIEIRDCVGENQMVE 374
           PQ+YSDDIIKGHVLTMLAAGTDTT+GT+EWAMSLLLNHP  M KAW EIR+CVGE+++VE
Sbjct: 308 PQHYSDDIIKGHVLTMLAAGTDTTTGTLEWAMSLLLNHPAIMNKAWSEIRECVGESRLVE 367

Query: 375 EGDVSELKYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFEVPKGTMLLVNAWGIHRDP 434
           EGDVS LKYL AIIYETLR+FPAAPLLVPHE SE+C +EGF++PKGTMLLVNAW IHRDP
Sbjct: 368 EGDVSSLKYLVAIIYETLRLFPAAPLLVPHESSEECRVEGFDIPKGTMLLVNAWAIHRDP 427

Query: 435 KVWEDPTSFQPERFLNWEGVETYKWIPFGMGRRACPGVALAQRSMGLTLATLIQCFEWKK 494
           +VWEDPTSF+PERFLNWE  E++KW+PFG+GRRACPG ALA R +GLTLATLIQCFEW++
Sbjct: 428 RVWEDPTSFRPERFLNWETAESHKWVPFGVGRRACPGAALAHRVVGLTLATLIQCFEWQR 487

Query: 495 VDEQEQIDLSEGSGITMPKAKALEAMCKPRTSMLHLLAQL 535
           V E E IDLS+G+GIT PKA  LEAMC+ R+SMLHLL+ L
Sbjct: 488 VGE-EPIDLSQGTGITTPKAIPLEAMCRARSSMLHLLSPL 519

BLAST of MELO3C016793.2 vs. NCBI nr
Match: XP_022936846.1 (isoflavone 3'-hydroxylase-like isoform X1 [Cucurbita moschata] >XP_022936847.1 isoflavone 3'-hydroxylase-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 771.2 bits (1990), Expect = 2.4e-219
Identity = 383/516 (74.22%), Postives = 444/516 (86.05%), Query Frame = 0

Query: 19  MEPHTITWFSLSLLLITTLILLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKEPFHRVLHN 78
           ME +TITW SLSLL   TL L+ KLRK ++ ++ PP+PPSLP+IGHLHLLK+PFHRVL +
Sbjct: 1   MEANTITWLSLSLLF--TLSLIYKLRK-QSPRRCPPSPPSLPLIGHLHLLKQPFHRVLQD 60

Query: 79  LSNKYGPILSLTIGSRPVLVVSSPTAVRECFTKNDIVFANRPRLISGKYINYNYTAVGFA 138
           LS+K+GPILSL+IGSRP++V+SSPTAVRECFTKNDI FANRPRL+SGKY+NYNYT V  A
Sbjct: 61  LSDKHGPILSLSIGSRPMVVISSPTAVRECFTKNDIAFANRPRLLSGKYMNYNYTGVAVA 120

Query: 139 PYGQHWRNMRRIATTELLSNHRLNMYLNIRVEELKLWVKNNLYKWGSGGDFVVVDMKSKL 198
            YG HWRNMRRIATTELLSNHRLN YLNIRV+ELKLWVKN   + G   DFV ++M+SKL
Sbjct: 121 SYGHHWRNMRRIATTELLSNHRLNAYLNIRVQELKLWVKNLYGETGESSDFVRLEMQSKL 180

Query: 199 KELSFNTVMRMISGKRYFGVEVEDVEEAFEFREIMKELLELSGATNAADFLKILRVFDLE 258
            ELSFNTVMRM+SGKRYFGV+VED         IMKELL LSGA+N ADFL ILR+FD +
Sbjct: 181 NELSFNTVMRMVSGKRYFGVDVEDXXXXXXXXXIMKELLHLSGASNPADFLPILRLFDYQ 240

Query: 259 GVKKRMMKASGRADVFLQKLIDEERKKKRVSRWPEEKQKQAKTSMIRTLLSLQESQPQYY 318
           G++KRM+KASGRAD+FLQ LID ER ++R SRWPEE  K    +MI++LLS QESQPQYY
Sbjct: 241 GLEKRMVKASGRADLFLQNLIDCER-ERRASRWPEE--KHGNKTMIQSLLSFQESQPQYY 300

Query: 319 SDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPMTMKKAWIEIRDCVGENQMVEEGDV 378
           SDDIIKGHVLTMLAAGTDTT+GTIEWAMSLLLNHP  M KAW EIR+CVGE+++VEEGDV
Sbjct: 301 SDDIIKGHVLTMLAAGTDTTTGTIEWAMSLLLNHPTIMDKAWSEIRECVGESRLVEEGDV 360

Query: 379 SELKYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFEVPKGTMLLVNAWGIHRDPKVWE 438
           S LKY+EAIIYETLR+FP APLLVPHE SE+C IEGF++PKGTMLLVNAW IHRDP++WE
Sbjct: 361 SSLKYVEAIIYETLRLFPVAPLLVPHESSEECRIEGFDIPKGTMLLVNAWAIHRDPQLWE 420

Query: 439 DPTSFQPERFLNWEGVETYKWIPFGMGRRACPGVALAQRSMGLTLATLIQCFEWKKVDEQ 498
           DPTSF+PERFLNWE  E++KW+PFG+GRRACPGVALA R +GLTLATLIQCFEW++V E 
Sbjct: 421 DPTSFRPERFLNWEAAESHKWVPFGVGRRACPGVALAHRVVGLTLATLIQCFEWQRVGE- 480

Query: 499 EQIDLSEGSGITMPKAKALEAMCKPRTSMLHLLAQL 535
           E IDLSEG+GITMPKA  L AMC+ R+SMLHLL+ L
Sbjct: 481 EPIDLSEGTGITMPKAIPLVAMCRARSSMLHLLSPL 509

BLAST of MELO3C016793.2 vs. NCBI nr
Match: XP_023535658.1 (isoflavone 2'-hydroxylase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 769.2 bits (1985), Expect = 9.0e-219
Identity = 382/519 (73.60%), Postives = 442/519 (85.16%), Query Frame = 0

Query: 16  YPSMEPHTITWFSLSLLLITTLILLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKEPFHRV 75
           Y SME  TITW SLSLL   TLIL+ KLRK ++ +K PP+PPSLP+IGHLHLLK+PFHRV
Sbjct: 17  YSSMEASTITWLSLSLLF--TLILIHKLRK-QSPRKCPPSPPSLPLIGHLHLLKQPFHRV 76

Query: 76  LHNLSNKYGPILSLTIGSRPVLVVSSPTAVRECFTKNDIVFANRPRLISGKYINYNYTAV 135
           L +LSNK+GPILSL+IGSRP++V+SSPTAVRECFTKNDI FANRPRL+SGKY+NYNYT V
Sbjct: 77  LQDLSNKHGPILSLSIGSRPMVVISSPTAVRECFTKNDIAFANRPRLLSGKYMNYNYTGV 136

Query: 136 GFAPYGQHWRNMRRIATTELLSNHRLNMYLNIRVEELKLWVKNNLYKWGSGGDFVVVDMK 195
            FA YG HWRNMRRIATTELLSNHRLN YLNIRV+ELKLWVKN     G   DFV ++M+
Sbjct: 137 AFASYGHHWRNMRRIATTELLSNHRLNTYLNIRVQELKLWVKNLYGATGESSDFVRLEMQ 196

Query: 196 SKLKELSFNTVMRMISGKRYFGVEVEDVEEAFEFREIMKELLELSGATNAADFLKILRVF 255
           SKL ELSFNTVMRM+SGKRYFGV+VE             ELL+LSGA+N ADFL ILR+F
Sbjct: 197 SKLNELSFNTVMRMVSGKRYFGVDVEXXXXXXXXXXXXXELLQLSGASNPADFLPILRLF 256

Query: 256 DLEGVKKRMMKASGRADVFLQKLIDEERKKKRVSRWPEEKQKQAKTSMIRTLLSLQESQP 315
           D +G++KRM+KASGRAD+FLQ LID ER ++R S WPEE  K    +MI++LLSLQESQP
Sbjct: 257 DYQGLEKRMVKASGRADLFLQNLIDCER-ERRASPWPEE--KHGNKTMIQSLLSLQESQP 316

Query: 316 QYYSDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPMTMKKAWIEIRDCVGENQMVEE 375
           QYYSDDIIKGHVLTMLAAGTDTT+GTIEWAMSLLLNHP  M KAW EIR+CVGE+++VEE
Sbjct: 317 QYYSDDIIKGHVLTMLAAGTDTTTGTIEWAMSLLLNHPTIMNKAWSEIRECVGESRLVEE 376

Query: 376 GDVSELKYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFEVPKGTMLLVNAWGIHRDPK 435
           GDVS LKY+EAIIYETLR+FP APLLVPHE SE+C IEGF++PKGTMLLVNAW IHRDP+
Sbjct: 377 GDVSSLKYVEAIIYETLRLFPVAPLLVPHESSEECRIEGFDIPKGTMLLVNAWAIHRDPQ 436

Query: 436 VWEDPTSFQPERFLNWEGVETYKWIPFGMGRRACPGVALAQRSMGLTLATLIQCFEWKKV 495
           +WEDPTSF+PER LNWE  E++KW+PFG+GRRACPG ALA R +GLTLATLIQCFEW++V
Sbjct: 437 LWEDPTSFRPERLLNWESAESHKWVPFGVGRRACPGAALAHRVVGLTLATLIQCFEWQRV 496

Query: 496 DEQEQIDLSEGSGITMPKAKALEAMCKPRTSMLHLLAQL 535
            E E +DLSEG+GIT+PKA  LEAMC+ R+SMLHLL+ L
Sbjct: 497 GE-EPVDLSEGTGITIPKAIPLEAMCRARSSMLHLLSLL 528

BLAST of MELO3C016793.2 vs. TAIR10
Match: AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8)

HSP 1 Score: 476.9 bits (1226), Expect = 1.7e-134
Identity = 248/515 (48.16%), Postives = 355/515 (68.93%), Query Frame = 0

Query: 19  MEPHTITWFSLSLLLITTLILLSKLRKVRNQKKLPPTPP-SLPIIGHLHLLKEPFHRVLH 78
           ME  T+  FS+  ++++ + L+ KL++  N   LPP+P  SLP+IGHL LLK P HR   
Sbjct: 1   METKTLI-FSILFVVLSLIYLIGKLKRKPN---LPPSPAWSLPVIGHLRLLKPPIHRTFL 60

Query: 79  NLSNKY--GPILSLTIGSRPVLVVSSPTAVRECFTKNDIVFANRPRLISGKYINYNYTAV 138
           +LS      PI SL +G+R V V SS +   ECFTKND+V ANRP  I  K++ Y+YT +
Sbjct: 61  SLSQSLNNAPIFSLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTM 120

Query: 139 GFAPYGQHWRNMRRIATTELLSNHRLNMYLNIRVEELKLWVKNNLYKWGSGGDFVVVDMK 198
             A YG HWRN+RRI + E+ SNHRLN +L+IR +E++  V      +    +FV VDMK
Sbjct: 121 IAASYGDHWRNLRRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRNFSQ--EFVKVDMK 180

Query: 199 SKLKELSFNTVMRMISGKRYFGVEVEDVEEAFEFREIMKELLELSGATNAADFLKILRVF 258
           S L +L+FN ++RM++GKRY+G  VED  EA   R+++ +++  +GA NA D+L +LR+ 
Sbjct: 181 SMLSDLTFNNILRMVAGKRYYGDGVEDDPEAKRVRQLIADVVACAGAGNAVDYLPVLRL- 240

Query: 259 DLEGVKKRMMKASGRADVFLQKLIDEERKKKRVSRWPEEKQKQAKTSMIRTLLSLQESQP 318
            +   + R+ K +GR D FLQ L+DE+R           + K+   +MI  LL+LQESQP
Sbjct: 241 -VSDYETRVKKLAGRLDEFLQGLVDEKR-----------EAKEKGNTMIDHLLTLQESQP 300

Query: 319 QYYSDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPMTMKKAWIEIRDCVGENQMVEE 378
            Y++D IIKG++L ++ AGTDT++ T+EWA+S +LNHP  + KA  EI   +G +++++E
Sbjct: 301 DYFTDRIIKGNMLALILAGTDTSAVTLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDE 360

Query: 379 GDVSELKYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFEVPKGTMLLVNAWGIHRDPK 438
            D+S L YL+ I+ ETLR++PAAP+L+PH  SEDC + G+++P+GT+LL N W IHRDP+
Sbjct: 361 SDISNLPYLQNIVSETLRLYPAAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQ 420

Query: 439 VWEDPTSFQPERFLNWEGVETYKWIPFGMGRRACPGVALAQRSMGLTLATLIQCFEWKKV 498
           +W+DP SF+PERF   EG E  K +PFG+GRRACPG  LA R + LTL +LIQC EW+K+
Sbjct: 421 LWDDPMSFKPERF-EKEG-EAQKLMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKI 480

Query: 499 DEQEQIDLSEGSGITMPKAKALEAMCKPRTSMLHL 531
              E++D+SEG G+TMPKAK LEAMC+ R S++ +
Sbjct: 481 G--EEVDMSEGKGVTMPKAKPLEAMCRARPSVVKI 492

BLAST of MELO3C016793.2 vs. TAIR10
Match: AT4G37340.1 (cytochrome P450, family 81, subfamily D, polypeptide 3)

HSP 1 Score: 453.4 bits (1165), Expect = 2.0e-127
Identity = 248/510 (48.63%), Postives = 339/510 (66.47%), Query Frame = 0

Query: 19  MEPHTITWFSLSLLLITTLILLSKLRKVRNQKKLPPTPP-SLPIIGHLHLLKEPFHRVLH 78
           ME  T+ +  L + L  T I    + +++ +  LPP+P  +LP+IGHL LLK P HRV  
Sbjct: 1   MEGQTLIFTFLFISLSLTFI----IGRIKRRPNLPPSPSWALPVIGHLRLLKPPLHRVFL 60

Query: 79  NLSNKYG--PILSLTIGSRPVLVVSSPTAVRECFTKNDIVFANRPRLISGKYINYNYTAV 138
           ++S   G  PI+SL +G+R V VVSS +   ECFTKND+V ANR   ++ K+I+Y  T V
Sbjct: 61  SVSESLGDAPIISLRLGNRLVFVVSSHSLAEECFTKNDVVLANRFNSLASKHISYGCTTV 120

Query: 139 GFAPYGQHWRNMRRIATTELLSNHRLNMYLNIRVEELKLWVKNNLYKWGSGGDFVVVDMK 198
             A YG HWRN+RRI   E+ S HRLN + +IR +E+   +        S  +F  V+MK
Sbjct: 121 VTASYGDHWRNLRRIGAVEIFSAHRLNSFSSIRRDEIHRLIA--CLSRNSSLEFTKVEMK 180

Query: 199 SKLKELSFNTVMRMISGKRYFGVEVEDVEEAFEFREIMKELLELSGATNAADFLKILRVF 258
           S    L+FN ++RM++GK Y+G   ED  EA   RE++ E +   GA N AD+L IL   
Sbjct: 181 SMFSNLTFNNIIRMLAGKCYYGDGAEDDPEAKRVRELIAEGMGCFGAGNTADYLPILTW- 240

Query: 259 DLEGVKKRMMKASGRADVFLQKLIDEERKKKRVSRWPEEKQKQAKTSMIRTLLSLQESQP 318
            + G +KR+ K + R D FLQ L+DE R         E K+K+  T M+  LL LQE+QP
Sbjct: 241 -ITGSEKRIKKIASRLDEFLQGLVDERR---------EGKEKRQNT-MVDHLLCLQETQP 300

Query: 319 QYYSDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPMTMKKAWIEIRDCVGENQMVEE 378
           +YY+D+IIKG +L+++ AGTDT++ T+EW +S LLNHP  + KA  EI + VG N++VEE
Sbjct: 301 EYYTDNIIKGIMLSLILAGTDTSAVTLEWTLSALLNHPQILSKARDEIDNKVGLNRLVEE 360

Query: 379 GDVSELKYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFEVPKGTMLLVNAWGIHRDPK 438
            D+S L YL+ I+ E+LR++PA+PLLVPH  SEDC + G+ +P+GTMLL NAW IHRDPK
Sbjct: 361 SDLSHLPYLQNIVSESLRLYPASPLLVPHVASEDCKVGGYHMPRGTMLLTNAWAIHRDPK 420

Query: 439 VWEDPTSFQPERFLNWEGVETYKWIPFGMGRRACPGVALAQRSMGLTLATLIQCFEWKKV 498
           +W+DPTSF+PERF   EG E  K + FG+GRRACPG  LAQR   LT+ +LIQCFEW+++
Sbjct: 421 IWDDPTSFKPERF-EKEG-EAQKLLGFGLGRRACPGSGLAQRLASLTIGSLIQCFEWERI 480

Query: 499 DEQEQIDLSE-GSGITMPKAKALEAMCKPR 525
            E E++D++E G G+ MPKA  L AMCK R
Sbjct: 481 GE-EEVDMTEGGGGVIMPKAIPLVAMCKAR 489

BLAST of MELO3C016793.2 vs. TAIR10
Match: AT4G37360.1 (cytochrome P450, family 81, subfamily D, polypeptide 2)

HSP 1 Score: 451.8 bits (1161), Expect = 5.8e-127
Identity = 238/501 (47.50%), Postives = 341/501 (68.06%), Query Frame = 0

Query: 27  FSLSLLLITTLILLSKLRKVRNQKKLPPTPP-SLPIIGHLHLLKEPFHRVLHNLSNKYG- 86
           F+   ++++ + L+ ++++  N   LPP+P  +LP+IGHL LLK P HRV  ++S   G 
Sbjct: 8   FTFCFIVLSLIFLIGRIKRKLN---LPPSPAWALPVIGHLRLLKPPLHRVFLSVSQSLGD 67

Query: 87  -PILSLTIGSRPVLVVSSPTAVRECFTKNDIVFANRPRLISGKYINYNYTAVGFAPYGQH 146
            PI+SL +G+R + VVSS +   ECFTKND++ ANR   IS K+I+Y  + V  A Y +H
Sbjct: 68  APIISLRLGNRLLFVVSSHSIAEECFTKNDVILANRQTTISTKHISYGNSTVVSASYSEH 127

Query: 147 WRNMRRIATTELLSNHRLNMYLNIRVEELKLWVKNNLYKWGSGGDFVVVDMKSKLKELSF 206
           WRN+RRI   E+ S HRLN + +IR +E++  +   L     G  F  V+MKS   +L+F
Sbjct: 128 WRNLRRIGALEIFSAHRLNSFSSIRRDEIRRLIGRLLRNSSYG--FTKVEMKSMFSDLTF 187

Query: 207 NTVMRMISGKRYFGVEVEDVEEAFEFREIMKELLELSGATNAADFLKILRVFDLEGVKKR 266
           N ++RM++GK Y+G   ED  EA   R ++ E +  SG  NAAD++ IL        + R
Sbjct: 188 NNIIRMLAGKCYYGDGKEDDPEAKRVRTLIAEAMSSSGPGNAADYIPILTWITYS--ETR 247

Query: 267 MMKASGRADVFLQKLIDEERKKKRVSRWPEEKQKQAKTSMIRTLLSLQESQPQYYSDDII 326
           + K +GR D FLQ L+DE+R         E K+K+  T M+  LL LQE+QP+YY D II
Sbjct: 248 IKKLAGRLDEFLQGLVDEKR---------EGKEKKENT-MVDHLLCLQETQPEYYMDRII 307

Query: 327 KGHVLTMLAAGTDTTSGTIEWAMSLLLNHPMTMKKAWIEIRDCVGENQMVEEGDVSELKY 386
           KG +L+++A GTDTT+ T+EWA+S LLN+P  + KA  EI   +G ++++EE D+  L Y
Sbjct: 308 KGTMLSLIAGGTDTTAVTLEWALSSLLNNPEVLNKARDEIDRMIGVDRLLEESDIPNLPY 367

Query: 387 LEAIIYETLRMFPAAPLLVPHECSEDCSIEGFEVPKGTMLLVNAWGIHRDPKVWEDPTSF 446
           L+ I+ ETLR++PAAP+L+PH  S+DC + G+++P+GTMLL NAW IHRDP +W+DPTSF
Sbjct: 368 LQNIVSETLRLYPAAPMLLPHVASKDCKVGGYDMPRGTMLLTNAWAIHRDPLLWDDPTSF 427

Query: 447 QPERFLNWEGVETYKWIPFGMGRRACPGVALAQRSMGLTLATLIQCFEWKKVDEQEQIDL 506
           +PERF   EG E  K +PFG+GRRACPG  LAQR + L+L +LIQCFEW+++ E E++D+
Sbjct: 428 KPERF-EKEG-EAKKLMPFGLGRRACPGSGLAQRLVTLSLGSLIQCFEWERIGE-EEVDM 487

Query: 507 SEGSGITMPKAKALEAMCKPR 525
           +EG G+TMPKA+ LEAMC+ R
Sbjct: 488 TEGPGLTMPKARPLEAMCRAR 488

BLAST of MELO3C016793.2 vs. TAIR10
Match: AT4G37330.1 (cytochrome P450, family 81, subfamily D, polypeptide 4)

HSP 1 Score: 448.0 bits (1151), Expect = 8.3e-126
Identity = 242/500 (48.40%), Postives = 330/500 (66.00%), Query Frame = 0

Query: 29  LSLLLITTLILLSKLRKVRNQKKLPPTPP-SLPIIGHLHLLKEPFHRVLHNLSNKYG--P 88
           LS L + TL +   L + + +  LPP+P  SLP+IGHLHLLK P HR   +LS   G  P
Sbjct: 9   LSFLSLFTLAIF-LLTRSKRKLNLPPSPAISLPVIGHLHLLKPPLHRTFLSLSKSIGNAP 68

Query: 89  ILSLTIGSRPVLVVSSPTAVRECFTKNDIVFANRPRLISGKYINYNYTAVGFAPYGQHWR 148
           +  L +G+R V V+SS +   ECFTKND+V ANRP+    K++ YN T +  A YG HWR
Sbjct: 69  VFHLRLGNRLVYVISSRSIAEECFTKNDVVLANRPKFTISKHLGYNATYLLSASYGDHWR 128

Query: 149 NMRRIATTELLSNHRLNMYLNIRVEELKLWVKNNLYKWGSGGDFVVVDMKSKLKELSFNT 208
           N+RRIA  E+ S HRLN +L IR +E++  + +      S   FV V+MK+ L  L+ NT
Sbjct: 129 NLRRIAAVEIFSTHRLNSFLYIRKDEIRRLISH--LSRDSLHGFVEVEMKTLLTNLASNT 188

Query: 209 VMRMISGKRYFGVEVEDVEEAFEFREIMKELLELSGATNAADFLKILRVFDLEGVKKRMM 268
            +RM++GKRYFG   ED ++A   + ++ E +  +GA N  D+L ILR   +   +KR+ 
Sbjct: 189 TIRMLAGKRYFG---EDNDDAKLVKNLVSEAVTSAGAGNPIDYLSILRW--VSSYEKRIK 248

Query: 269 KASGRADVFLQKLIDEERKKKRVSRWPEEKQKQAKTSMIRTLLSLQESQPQYYSDDIIKG 328
               R D FLQKL+DE+R            +K+   +MI  LL+LQ+ QP YY+D IIKG
Sbjct: 249 NLGNRFDTFLQKLVDEKR-----------AEKEKGETMIDHLLALQDIQPDYYTDVIIKG 308

Query: 329 HVLTMLAAGTDTTSGTIEWAMSLLLNHPMTMKKAWIEIRDCVGENQMVEEGDVSELKYLE 388
            +LT++ AGTDT+S T+EWAMS LLNHP  +KKA +EI + VG +++V+E D+  L YL+
Sbjct: 309 IILTLIIAGTDTSSVTLEWAMSNLLNHPEILKKARMEIDEKVGLDRLVDESDIVNLSYLQ 368

Query: 389 AIIYETLRMFPAAPLLVPHECSEDCSIEGFEVPKGTMLLVNAWGIHRDPKVWEDPTSFQP 448
           +I+ ETLRM+PA PLL+PH  SEDC + G+++P GTM+L NAW +HRDP+VWEDP  F+P
Sbjct: 369 SIVLETLRMYPAVPLLLPHLSSEDCKVGGYDIPSGTMVLTNAWAMHRDPEVWEDPEIFKP 428

Query: 449 ERFLNWEGVETYKWIPFGMGRRACPGVALAQRSMGLTLATLIQCFEWKKVDEQEQIDLSE 508
           ERF   EG E  K I FGMGRRACPG  LA R +   L +L+QCFEW++V E + +D++E
Sbjct: 429 ERF-EKEG-EAEKLISFGMGRRACPGAGLAHRLINQALGSLVQCFEWERVGE-DFVDMTE 486

Query: 509 GSGITMPKAKALEAMCKPRT 526
             G T+PKA  L AMCK R+
Sbjct: 489 DKGATLPKAIPLRAMCKARS 486

BLAST of MELO3C016793.2 vs. TAIR10
Match: AT5G36220.1 (cytochrome p450 81d1)

HSP 1 Score: 445.7 bits (1145), Expect = 4.1e-125
Identity = 246/513 (47.95%), Postives = 333/513 (64.91%), Query Frame = 0

Query: 19  MEPHTITWFSLSLLLITTLILLSKLRKVRNQKKLPPTPPS-LPIIGHLHLLKEPFHRVLH 78
           ME   I     S+  +  LI+  K  K + Q  LPP+PP  LPIIGHL LLK P HR L 
Sbjct: 1   MEETNIRVVLYSIFSLIFLIISFKFLKPKKQ-NLPPSPPGWLPIIGHLRLLKPPIHRTLR 60

Query: 79  NLS-----NKYGPILSLTIGSRPVLVVSS-PTAVRECFTKNDIVFANRPRLISGKYINYN 138
           + S     N  G ++SL +GSR V VVSS   A  ECF KND+V ANRP++I GK++ YN
Sbjct: 61  SFSETLDHNDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYN 120

Query: 139 YTAVGFAPYGQHWRNMRRIATTELLSNHRLNMYLNIRVEELKLWVKNNLYKWGSGGDFVV 198
            T +  APYG HWRN+RR+ T E+ S HRLN +L +R +E++  +        +G    V
Sbjct: 121 NTNMIAAPYGDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISR--LSRLAGTKKTV 180

Query: 199 VDMKSKLKELSFNTVMRMISGKRYFGVEVEDVEEAFEFREIMKELLELSGATNAADFLKI 258
           V++K  L +L+FN +MRM++GKRY+G E  D EEA   R+++ ++   + + NA D++ I
Sbjct: 181 VELKPMLMDLTFNNIMRMMTGKRYYGEETTDEEEAKRVRKLVADVGANTSSGNAVDYVPI 240

Query: 259 LRVFDLEGVKKRMMKASGRADVFLQKLIDEERKKKRVSRWPEEKQKQAKTSMIRTLLSLQ 318
           LR+F     + R+ K     D FLQ LID++R            Q++  T+MI  LL LQ
Sbjct: 241 LRLF--SSYENRVKKLGEETDKFLQGLIDDKR-----------GQQETGTTMIDHLLVLQ 300

Query: 319 ESQPQYYSDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPMTMKKAWIEIRDCVGENQ 378
           +S  +YY+D IIKG +L M+ AGT+T++ T+EWA+S LLNHP  + KA  EI + VG ++
Sbjct: 301 KSDIEYYTDQIIKGIILIMVIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDR 360

Query: 379 MVEEGDVSELKYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFEVPKGTMLLVNAWGIH 438
           ++EE D+SEL YL+ I+ ETLR+ PA PLLVPH  SEDC I  +++P+GT LLVNAW IH
Sbjct: 361 LIEEADLSELPYLKNIVLETLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIH 420

Query: 439 RDPKVWEDPTSFQPERFLNWEGVETYKWIPFGMGRRACPGVALAQRSMGLTLATLIQCFE 498
           RDP  W+DP SF+PERF   E  E  K + FG+GRRACPG  LAQR +GL L +LIQCFE
Sbjct: 421 RDPNTWDDPDSFKPERFEKEE--EAQKLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFE 480

Query: 499 WKKVDEQEQIDLSEGSGITMPKAKALEAMCKPR 525
           W++V   E +D+ EG G T+PKA  L+A+CK R
Sbjct: 481 WERVGNVE-VDMKEGVGNTVPKAIPLKAICKAR 494

BLAST of MELO3C016793.2 vs. Swiss-Prot
Match: sp|Q6WNQ8|C81E8_MEDTR (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)

HSP 1 Score: 507.7 bits (1306), Expect = 1.6e-142
Identity = 271/499 (54.31%), Postives = 344/499 (68.94%), Query Frame = 0

Query: 28  SLSLLLITTLILLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKEPFHRVLHNLSNKYGPIL 87
           SL  L+IT  +  +  RK +N   LPP P  LPIIG+LH LK+P H   H LS KYG I 
Sbjct: 11  SLFFLIITLKVFFNTSRKFKN---LPPGPQCLPIIGNLHQLKQPLHHTFHTLSQKYGQIF 70

Query: 88  SLTIGSRPVLVVSSPTAVRECFTKNDIVFANRPRLISGKYINYNYTAVGFAPYGQHWRNM 147
           SL  GSR V+VVSS T  +ECFTKNDIV ANRP  ++GKYI YN T V  +PYG HWRN+
Sbjct: 71  SLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGDHWRNL 130

Query: 148 RRIATTELLSNHRLNMYLNIRVEELKLWVKNNLYKWGSGGDFVVVDMKSKLKELSFNTVM 207
           RRI + E+LS+HRLN +L IR +E+   ++    K  +G  F  V+++    E++FNT+M
Sbjct: 131 RRILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQKSYNG--FTEVELRPMFSEMTFNTIM 190

Query: 208 RMISGKRYFG--VEVEDVEEAFEFREIMKELLELSGATNAADFLKILRVFDLEGVKKRMM 267
           RM+SGKRY+G   +V DVEEA  FR I+KE++ L GA N  DFL  LR FD +G++KR+ 
Sbjct: 191 RMVSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFDGLEKRLK 250

Query: 268 KASGRADVFLQKLIDEERKKKRVSRWPEEKQKQAKTSMIRTLLSLQESQPQYYSDDIIKG 327
           K S R D FLQ LIDE R  KR S            +MI  LL+ Q+SQP+YY+D IIKG
Sbjct: 251 KISKRTDAFLQGLIDEHRFGKRNS-----------NTMIDHLLTQQQSQPEYYTDQIIKG 310

Query: 328 HVLTMLAAGTDTTSGTIEWAMSLLLNHPMTMKKAWIEIRDCVGENQMVEEGDVSELKYLE 387
            ++ ML AGTDT+S TIEWAMS LLNHP  MKKA  E+   +G ++ V+E D+S+L YL+
Sbjct: 311 LMVVMLLAGTDTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGHDRQVDEHDISKLPYLQ 370

Query: 388 AIIYETLRMFPAAPLLVPHECSEDCSIEGFEVPKGTMLLVNAWGIHRDPKVWEDPTSFQP 447
           +I+YETLR+  AAPLLVPH  SED S+ G+ +P+ T+L+VNAW IHRDP +W DPT F+P
Sbjct: 371 SIVYETLRLHAAAPLLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDPNLWSDPTCFKP 430

Query: 448 ERFLNWEGVETYKWIPFGMGRRACPGVALAQRSMGLTLATLIQCFEWKKVDEQEQIDLSE 507
           ERF   EG E  K + FG+GRRACPG  L+QR+ GLTL  LIQCFEWK++ E E+ID+ E
Sbjct: 431 ERF-EKEG-EVNKLLSFGLGRRACPGENLSQRTEGLTLGLLIQCFEWKRIGE-EKIDMVE 490

Query: 508 GSGITMPKAKALEAMCKPR 525
             GIT  K  +L AMCK R
Sbjct: 491 AKGITAGKKTSLNAMCKVR 490

BLAST of MELO3C016793.2 vs. Swiss-Prot
Match: sp|Q6WNQ9|C81E9_MEDTR (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)

HSP 1 Score: 496.9 bits (1278), Expect = 2.8e-139
Identity = 262/497 (52.72%), Postives = 343/497 (69.01%), Query Frame = 0

Query: 29  LSLLLITTLILLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKEPFHRVLHNLSNKYGPILS 88
           LSL  I T+ +L K+   R  K LPP PP++PIIG+LH LK P HR    LS  YG I S
Sbjct: 8   LSLSFIITIKILLKITS-RRLKNLPPGPPTIPIIGNLHHLKHPLHRTFTTLSQTYGDIFS 67

Query: 89  LTIGSRPVLVVSSPTAVRECFTKNDIVFANRPRLISGKYINYNYTAVGFAPYGQHWRNMR 148
           L  GSR V+VVSSP+   ECFTKNDI+ ANRPR ++GKYI YNYT +G A YG HWRN+R
Sbjct: 68  LWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHWRNLR 127

Query: 149 RIATTELLSNHRLNMYLNIRVEELKLWVKNNLYKWGSGG-DFVVVDMKSKLKELSFNTVM 208
           RI T ++LSN+RLN +L +R +E    ++  L    S G  F  V+++ +L E++FN +M
Sbjct: 128 RITTIDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGFTKVELRPRLTEMTFNAMM 187

Query: 209 RMISGKRYFG--VEVEDVEEAFEFREIMKELLELSGATNAADFLKILRVFDLEGVKKRMM 268
           RMISGKRY+G   +V DVEEA +FREI+ E++ L GA N  DFL +LRV DL+ ++KR  
Sbjct: 188 RMISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLDNLEKRCK 247

Query: 269 KASGRADVFLQKLIDEERKKKRVSRWPEEKQKQAKTSMIRTLLSLQESQPQYYSDDIIKG 328
           + + R++ FL+ LI+E R+    S            +MI  LL L ESQP+YYSD +IKG
Sbjct: 248 RIAKRSNAFLEGLIEEHRRGNIHS---------DGGTMIDHLLKLSESQPEYYSDHLIKG 307

Query: 329 HVLTMLAAGTDTTSGTIEWAMSLLLNHPMTMKKAWIEIRDCVGENQMVEEGDVSELKYLE 388
            +  ML AGTDT++ TIEW MS LLNHP  +KKA  E+   +G+N++V+E D+S+L YL+
Sbjct: 308 LIQGMLLAGTDTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKLPYLQ 367

Query: 389 AIIYETLRMFPAAPLLVPHECSEDCSIEGFEVPKGTMLLVNAWGIHRDPKVWEDPTSFQP 448
            II ETLR+ P APLL+PH  SEDC+I  F VPK T++L N WGIHRDPK W D  SF+P
Sbjct: 368 NIISETLRLHPPAPLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDPKHWNDALSFKP 427

Query: 449 ERFLNWEGVETYKWIPFGMGRRACPGVALAQRSMGLTLATLIQCFEWKKVDEQEQIDLSE 508
           ERF   E  E  K + FG+GRRACPG++LAQR++G T+  LIQCFEW++ + +E++D+ E
Sbjct: 428 ERFEKEE--EVNKVMAFGLGRRACPGLSLAQRTVGFTVGLLIQCFEWER-ESEEKLDMME 487

Query: 509 GSGITMPKAKALEAMCK 523
           G GITMP    L AMCK
Sbjct: 488 GKGITMPMKIPLRAMCK 491

BLAST of MELO3C016793.2 vs. Swiss-Prot
Match: sp|P93147|C81E1_GLYEC (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 480.7 bits (1236), Expect = 2.1e-134
Identity = 242/503 (48.11%), Postives = 345/503 (68.59%), Query Frame = 0

Query: 24  ITWFSLSLLLITTLILLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKEPFHRVLHNLSNKY 83
           ++  S S+  +    + + + + R  K LPP PPSLPIIG+LH LK P HR    LS KY
Sbjct: 4   LSLLSYSVFYLALFFIFNIVIRARKFKNLPPGPPSLPIIGNLHHLKRPLHRTFKGLSEKY 63

Query: 84  GPILSLTIGSRPVLVVSSPTAVRECFTKNDIVFANRPRLISGKYINYNYTAVGFAPYGQH 143
           G + SL  GSR V+VVSS +  ++CFTKND+V ANRPR +SGKYI YNYT +G   YG+H
Sbjct: 64  GHVFSLWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEH 123

Query: 144 WRNMRRIATTELLSNHRLNMYLNIRVEELKLWVKNNLYKWGSGGDFVVVDMKSKLKELSF 203
           WRN+RRI   ++LSNHR+N +  IR +E +  +        S  +F  +++ S+L +++F
Sbjct: 124 WRNLRRITALDVLSNHRINSFSGIRRDETQRLITR--LADDSSTNFAEMELSSRLYDMTF 183

Query: 204 NTVMRMISGKRYFG--VEVEDVEEAFEFREIMKELLELSGATNAADFLKILRVFDLEGVK 263
           N +MRMISGKRY+G   +  D++EA +FR+++ ELL+LSGA N  DF+ +LR  D E ++
Sbjct: 184 NNIMRMISGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFENLE 243

Query: 264 KRMMKASGRADVFLQKLIDEERKKKRVSRWPEEKQKQAKTSMIRTLLSLQESQPQYYSDD 323
           KR+   SG+ D FL+ LI+E R K           K+   +MI  LL+LQ+SQP+YY+D 
Sbjct: 244 KRLKDISGKTDAFLRGLIEEHRTK-----------KERANTMIDHLLNLQDSQPEYYTDQ 303

Query: 324 IIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPMTMKKAWIEIRDCVGENQMVEEGDVSEL 383
           IIKG  L ML AGTD+++ T+EW+MS LLNHP  +KK   E+   VG++++V+E D+ +L
Sbjct: 304 IIKGLALAMLLAGTDSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQDRLVDESDLPKL 363

Query: 384 KYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFEVPKGTMLLVNAWGIHRDPKVWEDPT 443
            YL+ +I ETLR++  APLL+PH  S++C+I G++VP+ T++L+NAW IHRDP++W + T
Sbjct: 364 TYLKNVINETLRLYTPAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEAT 423

Query: 444 SFQPERFLNWEGVETYKWIPFGMGRRACPGVALAQRSMGLTLATLIQCFEWKKVDEQEQI 503
           +F+PERF     +E  K I FGMGRRACPG  LA R++ +TLA LIQCF+WK ++  ++I
Sbjct: 424 TFKPERFEKKGELE--KLIAFGMGRRACPGEGLAIRAISMTLALLIQCFDWKLIN-GDKI 483

Query: 504 DLSEGSGITMPKAKALEAMCKPR 525
           DL+E  G T+ K   L+AMCK R
Sbjct: 484 DLAERDGFTLTKLVPLKAMCKSR 490

BLAST of MELO3C016793.2 vs. Swiss-Prot
Match: sp|Q6WNR0|C81E7_MEDTR (Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1)

HSP 1 Score: 471.9 bits (1213), Expect = 9.7e-132
Identity = 237/503 (47.12%), Postives = 346/503 (68.79%), Query Frame = 0

Query: 24  ITWFSLSLLLITTLILLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKEPFHRVLHNLSNKY 83
           +++   SL  ++   ++  L + R  K LPP P SLPIIG+LH LK P +R    L+ KY
Sbjct: 4   LSYLCYSLFYLSIFFIIRLLFQSRKFKNLPPGPTSLPIIGNLHHLKRPLNRTFKALTEKY 63

Query: 84  GPILSLTIGSRPVLVVSSPTAVRECFTKNDIVFANRPRLISGKYINYNYTAVGFAPYGQH 143
           G ++SL  GSR V+VVSS +  +ECFTKND+V ANRPR +SGKYI YNYT +G   YG+H
Sbjct: 64  GNVISLWFGSRLVVVVSSLSEFQECFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEH 123

Query: 144 WRNMRRIATTELLSNHRLNMYLNIRVEELKLWVKNNLYKWGSGGDFVVVDMKSKLKELSF 203
           WRN+RRI + ++LSNHR+N +  IR +E +  +K       S   F  V++  +  +++F
Sbjct: 124 WRNLRRITSLDVLSNHRINNFAPIRRDETQRLIKK--LAEDSSTKFAEVELTFRFFDMTF 183

Query: 204 NTVMRMISGKRYFG--VEVEDVEEAFEFREIMKELLELSGATNAADFLKILRVFDLEGVK 263
           N +MRMISGKRY+G   ++ +V+EA +FR+++ ELL+LSGA N  DF+ +L+  D E ++
Sbjct: 184 NNIMRMISGKRYYGDDCDISEVQEASQFRDMVSELLQLSGANNKTDFMPLLKFLDFENLE 243

Query: 264 KRMMKASGRADVFLQKLIDEERKKKRVSRWPEEKQKQAKTSMIRTLLSLQESQPQYYSDD 323
           KR+ +   + DVFL  L+ E+R K           K+   +MI  LL++QESQP+YY+D 
Sbjct: 244 KRVKRIGEKNDVFLSGLLQEQRSK-----------KERTNTMIDHLLNMQESQPEYYTDT 303

Query: 324 IIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPMTMKKAWIEIRDCVGENQMVEEGDVSEL 383
           IIKG  L ML AGTD+++ T+EW MS +LN+P  +KK   E+   VG++++V+E D+ +L
Sbjct: 304 IIKGLCLAMLLAGTDSSAVTLEWTMSNILNYPEVLKKVRDEVDTHVGQDRLVDESDLPKL 363

Query: 384 KYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFEVPKGTMLLVNAWGIHRDPKVWEDPT 443
            YL  +IYETLR++  APLL+PH  +++C + G++VP+ T++L+NAW IHRDP+ W + T
Sbjct: 364 TYLRNVIYETLRLYTPAPLLLPHSTADECIMGGYKVPRDTIVLINAWAIHRDPETWSEAT 423

Query: 444 SFQPERFLNWEGVETYKWIPFGMGRRACPGVALAQRSMGLTLATLIQCFEWKKVDEQEQI 503
           +F+PERF + +G E  K I FGMGRRACPG  LA R++ +TLA L+QCF+WK++++ E+I
Sbjct: 424 TFKPERF-DKKG-ELEKMIAFGMGRRACPGEGLALRAISMTLALLVQCFDWKRIND-EKI 483

Query: 504 DLSEGSGITMPKAKALEAMCKPR 525
           D+SE  G TM K   L+AMCK R
Sbjct: 484 DMSERDGFTMTKLLPLKAMCKTR 490

BLAST of MELO3C016793.2 vs. Swiss-Prot
Match: sp|Q9FG65|C81D1_ARATH (Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1)

HSP 1 Score: 445.7 bits (1145), Expect = 7.4e-124
Identity = 246/513 (47.95%), Postives = 333/513 (64.91%), Query Frame = 0

Query: 19  MEPHTITWFSLSLLLITTLILLSKLRKVRNQKKLPPTPPS-LPIIGHLHLLKEPFHRVLH 78
           ME   I     S+  +  LI+  K  K + Q  LPP+PP  LPIIGHL LLK P HR L 
Sbjct: 1   MEETNIRVVLYSIFSLIFLIISFKFLKPKKQ-NLPPSPPGWLPIIGHLRLLKPPIHRTLR 60

Query: 79  NLS-----NKYGPILSLTIGSRPVLVVSS-PTAVRECFTKNDIVFANRPRLISGKYINYN 138
           + S     N  G ++SL +GSR V VVSS   A  ECF KND+V ANRP++I GK++ YN
Sbjct: 61  SFSETLDHNDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYN 120

Query: 139 YTAVGFAPYGQHWRNMRRIATTELLSNHRLNMYLNIRVEELKLWVKNNLYKWGSGGDFVV 198
            T +  APYG HWRN+RR+ T E+ S HRLN +L +R +E++  +        +G    V
Sbjct: 121 NTNMIAAPYGDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISR--LSRLAGTKKTV 180

Query: 199 VDMKSKLKELSFNTVMRMISGKRYFGVEVEDVEEAFEFREIMKELLELSGATNAADFLKI 258
           V++K  L +L+FN +MRM++GKRY+G E  D EEA   R+++ ++   + + NA D++ I
Sbjct: 181 VELKPMLMDLTFNNIMRMMTGKRYYGEETTDEEEAKRVRKLVADVGANTSSGNAVDYVPI 240

Query: 259 LRVFDLEGVKKRMMKASGRADVFLQKLIDEERKKKRVSRWPEEKQKQAKTSMIRTLLSLQ 318
           LR+F     + R+ K     D FLQ LID++R            Q++  T+MI  LL LQ
Sbjct: 241 LRLF--SSYENRVKKLGEETDKFLQGLIDDKR-----------GQQETGTTMIDHLLVLQ 300

Query: 319 ESQPQYYSDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPMTMKKAWIEIRDCVGENQ 378
           +S  +YY+D IIKG +L M+ AGT+T++ T+EWA+S LLNHP  + KA  EI + VG ++
Sbjct: 301 KSDIEYYTDQIIKGIILIMVIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDR 360

Query: 379 MVEEGDVSELKYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFEVPKGTMLLVNAWGIH 438
           ++EE D+SEL YL+ I+ ETLR+ PA PLLVPH  SEDC I  +++P+GT LLVNAW IH
Sbjct: 361 LIEEADLSELPYLKNIVLETLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIH 420

Query: 439 RDPKVWEDPTSFQPERFLNWEGVETYKWIPFGMGRRACPGVALAQRSMGLTLATLIQCFE 498
           RDP  W+DP SF+PERF   E  E  K + FG+GRRACPG  LAQR +GL L +LIQCFE
Sbjct: 421 RDPNTWDDPDSFKPERFEKEE--EAQKLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFE 480

Query: 499 WKKVDEQEQIDLSEGSGITMPKAKALEAMCKPR 525
           W++V   E +D+ EG G T+PKA  L+A+CK R
Sbjct: 481 WERVGNVE-VDMKEGVGNTVPKAIPLKAICKAR 494

BLAST of MELO3C016793.2 vs. TrEMBL
Match: tr|A0A1S3BWG9|A0A1S3BWG9_CUCME (LOW QUALITY PROTEIN: cytochrome P450 81E8-like OS=Cucumis melo OX=3656 GN=LOC103493891 PE=3 SV=1)

HSP 1 Score: 1036.6 bits (2679), Expect = 2.0e-299
Identity = 517/521 (99.23%), Postives = 518/521 (99.42%), Query Frame = 0

Query: 14  MTYPSMEPHTITWFSLSLLLITTLILLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKEPFH 73
           MTYPSMEPHTITWFSLSLLLITTLILLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKEPFH
Sbjct: 1   MTYPSMEPHTITWFSLSLLLITTLILLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKEPFH 60

Query: 74  RVLHNLSNKYGPILSLTIGSRPVLVVSSPTAVRECFTKNDIVFANRPRLISGKYINYNYT 133
           RVLHNLSNKYGPILSLTIGSRPVLVVSSPTAVRECFTKNDIVFANRPRLISGKYINYNYT
Sbjct: 61  RVLHNLSNKYGPILSLTIGSRPVLVVSSPTAVRECFTKNDIVFANRPRLISGKYINYNYT 120

Query: 134 AVGFAPYGQHWRNMRRIATTELLSNHRLNMYLNIRVEELKLWVKNNLYKWGSGGDFVVVD 193
           AVGFAPYGQHWRNMRRIATTELLSNHRLNMYLNIRVEELKLWVKNNLYKWGSGGDFVVVD
Sbjct: 121 AVGFAPYGQHWRNMRRIATTELLSNHRLNMYLNIRVEELKLWVKNNLYKWGSGGDFVVVD 180

Query: 194 MKSKLKELSFNTVMRMISGKRYFGVEVEDVEEAFEFREIMKELLELSGATNAADFLKILR 253
           MKSKLKELSFNTVMRMISGKRYFGVEVEDVEEAFEFREIMKELLELSGATNAADFLKILR
Sbjct: 181 MKSKLKELSFNTVMRMISGKRYFGVEVEDVEEAFEFREIMKELLELSGATNAADFLKILR 240

Query: 254 VFDLEGVKKRMMKASGRADVFLQKLIDEERKKKRVSRWPEEKQKQAKTSMIRTLLSLQES 313
           VFDLEGVKKRMMKASGRADVFLQKLIDEERKK+   RWPEEKQKQAKTSMIRTLLSLQES
Sbjct: 241 VFDLEGVKKRMMKASGRADVFLQKLIDEERKKE--FRWPEEKQKQAKTSMIRTLLSLQES 300

Query: 314 QPQYYSDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPMTMKKAWIEIRDCVGENQMV 373
           QPQYYSDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPMTMKKAWIEIRDCVGENQMV
Sbjct: 301 QPQYYSDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPMTMKKAWIEIRDCVGENQMV 360

Query: 374 EEGDVSELKYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFEVPKGTMLLVNAWGIHRD 433
           EEGDVSELKYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFEVPKGTMLLVNAWGIHRD
Sbjct: 361 EEGDVSELKYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFEVPKGTMLLVNAWGIHRD 420

Query: 434 PKVWEDPTSFQPERFLNWEGVETYKWIPFGMGRRACPGVALAQRSMGLTLATLIQCFEWK 493
           PKVWEDPTSFQPERFLNWEGVETYKWIPFGMGRRACPGVALAQRSMGLTLATLIQCFEWK
Sbjct: 421 PKVWEDPTSFQPERFLNWEGVETYKWIPFGMGRRACPGVALAQRSMGLTLATLIQCFEWK 480

Query: 494 KVDEQEQIDLSEGSGITMPKAKALEAMCKPRTSMLHLLAQL 535
           KVDEQEQIDLSEGSGITMPKAKALEAMCKPRTSMLHLLAQL
Sbjct: 481 KVDEQEQIDLSEGSGITMPKAKALEAMCKPRTSMLHLLAQL 519

BLAST of MELO3C016793.2 vs. TrEMBL
Match: tr|A0A0A0L2I1|A0A0A0L2I1_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G641760 PE=3 SV=1)

HSP 1 Score: 602.8 bits (1553), Expect = 7.4e-169
Identity = 303/514 (58.95%), Postives = 380/514 (73.93%), Query Frame = 0

Query: 29  LSLLLITTLILLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKEPFHRVLHNLSNKYGPILS 88
           L  + +T+L L+SK R+ ++ K LPP+PPSLP+IGHLHLLKEPFH++L + S KYGP+ S
Sbjct: 22  LLAIFLTSLFLISKFRRTQHTKLLPPSPPSLPVIGHLHLLKEPFHQMLQDFSKKYGPVFS 81

Query: 89  LTIGSRPVLVVSSPTAVRECFTKNDIVFANRPRLISGKYINYNYTAVGFAPYGQHWRNMR 148
           LT+G RP++VVSSP+AV+ECFTKNDIVFA+RPR++SGK +NY+YTAVG   YGQHWR MR
Sbjct: 82  LTLGFRPLVVVSSPSAVQECFTKNDIVFASRPRMLSGKILNYDYTAVGATAYGQHWRIMR 141

Query: 149 RIATTELLSNHRLNMYLNIRVEELKLWVKNNLY------KWGSGGDFVVVDMKSKLKELS 208
           RIATTELLS  RLN    IR EE+KLWVK  LY      +   GG    V ++SKL ELS
Sbjct: 142 RIATTELLSTKRLNDLSKIREEEVKLWVK-RLYGSVTRAEVNKGGR---VGLRSKLTELS 201

Query: 209 FNTVMRMISGKRYFGVEVE--DVEEAFEFREIMKELLELSGATNAADFLKILRVFDLEGV 268
           FN  + +++G+RYFG E+E  + EEA E +E MK++  LSGA+  ADFL +L++ D +G 
Sbjct: 202 FNIALMLMAGRRYFGDEMEAGESEEAKELKETMKQVPLLSGASYPADFLSVLKLIDFQGF 261

Query: 269 KKRMMKASGRADVFLQKLIDEERKKKRVSRWPEEKQKQAKTSMIRTLLSLQESQPQYYSD 328
           +KR+ K     D F Q L+DE RK K    W E K      +MI ++L LQES P+YY D
Sbjct: 262 RKRLAKVRVDGDAFAQSLVDERRKLK--GSWCERK------TMIDSMLCLQESDPEYYKD 321

Query: 329 DIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPMTMKKAWIEIRDCVGENQMVEEGDVSE 388
           DIIKG  LT+LAAGTDTT+ T+EWAMSLLLNHP  ++KAW EI   VG+ +++ E D ++
Sbjct: 322 DIIKGQFLTLLAAGTDTTASTLEWAMSLLLNHPTVLEKAWTEINQVVGDQRLIREADTNK 381

Query: 389 LKYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFEVPKGTMLLVNAWGIHRDPKVWEDP 448
           L YL+AII ET R+FPA P+LVP E SEDC+I GF++PKGTML+VNAW +HRDP VWEDP
Sbjct: 382 LHYLQAIINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAVHRDPNVWEDP 441

Query: 449 TSFQPERFLNWEGVETYKWIPFGMGRRACPGVALAQRSMGLTLATLIQCFEWKKVDEQEQ 508
             F+PERF   E VE  K +PFGMGRRACPG  LA R + L LATLIQCFEW K+D Q+ 
Sbjct: 442 NCFKPERFKQGETVEVNKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEWGKLDNQKD 501

Query: 509 IDLSEGSGITMPKAKALEAMCKPRTSMLHLLAQL 535
           +DL  G G+TMPKA  LEAMCKPR S+ HLL+QL
Sbjct: 502 VDLCPGIGLTMPKAIPLEAMCKPRASVTHLLSQL 523

BLAST of MELO3C016793.2 vs. TrEMBL
Match: tr|A0A1S3BTN1|A0A1S3BTN1_CUCME (isoflavone 2'-hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103493583 PE=3 SV=1)

HSP 1 Score: 596.7 bits (1537), Expect = 5.3e-167
Identity = 302/516 (58.53%), Postives = 378/516 (73.26%), Query Frame = 0

Query: 27  FSLSLLLITTLILLSKLRKVRNQK-KLPPTPPSLPIIGHLHLLKEPFHRVLHNLSNKYGP 86
           F L  + +TTL L+SK R+  + K  LPP+PPSLP+IGHLHLLKEPFH++L +LS KYGP
Sbjct: 18  FPLLAIFLTTLFLISKFRRSEHTKLLLPPSPPSLPVIGHLHLLKEPFHQMLQDLSLKYGP 77

Query: 87  ILSLTIGSRPVLVVSSPTAVRECFTKNDIVFANRPRLISGKYINYNYTAVGFAPYGQHWR 146
           + SLT+G RP++VVSSP+AV ECFTKNDIVFA+RPR++SGK +NY+YTAVG   YGQHWR
Sbjct: 78  VFSLTLGFRPLVVVSSPSAVHECFTKNDIVFASRPRMLSGKILNYDYTAVGATAYGQHWR 137

Query: 147 NMRRIATTELLSNHRLNMYLNIRVEELKLWVKN-----NLYKWGSGGDFVVVDMKSKLKE 206
            MRRIATTELLS  RLN +  +R EE+KLWVK       + +   GG    V ++SKL E
Sbjct: 138 IMRRIATTELLSTKRLNDFSKLREEEVKLWVKRLYGSVTIREVQKGGR---VGLRSKLTE 197

Query: 207 LSFNTVMRMISGKRYFGVEVE--DVEEAFEFREIMKELLELSGATNAADFLKILRVFDLE 266
           LSFN  + + +G+RYFG EVE  + EEA E +E MK++  LSGA+  ADFL +L++ D +
Sbjct: 198 LSFNIALMLTAGRRYFGEEVEEGESEEAKELKETMKQVPLLSGASYPADFLSVLKLIDFQ 257

Query: 267 GVKKRMMKASGRADVFLQKLIDEERKKKRVSRWPEEKQKQAKTSMIRTLLSLQESQPQYY 326
           G +KR+ K     D F Q L+DE R+ K    W E K      +MI  +LSLQES P+YY
Sbjct: 258 GFRKRLAKVRVDGDAFAQSLVDERRELK--GSWCERK------TMIDAMLSLQESDPEYY 317

Query: 327 SDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPMTMKKAWIEIRDCVGENQMVEEGDV 386
            DDIIKG  LT+LAAGTDT + T+EWAMSLLLNHP+ M KAW EI   VG+ ++V E D 
Sbjct: 318 KDDIIKGQFLTLLAAGTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVGDQRLVREADT 377

Query: 387 SELKYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFEVPKGTMLLVNAWGIHRDPKVWE 446
           + L YL++II ET R+FPA P+LVP E SEDC+I GF++PKGTML+VNAW +HR+P+VWE
Sbjct: 378 NNLHYLQSIINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAVHRNPEVWE 437

Query: 447 DPTSFQPERFLNWEGVETYKWIPFGMGRRACPGVALAQRSMGLTLATLIQCFEWKKVDEQ 506
           DP  F+PERF   E VE  K +PFGMGRRACPG  LA R + L LATLIQCFEW K+D +
Sbjct: 438 DPNCFKPERFKQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEWGKLDNK 497

Query: 507 EQIDLSEGSGITMPKAKALEAMCKPRTSMLHLLAQL 535
           + +DL  G G+TMPKA  LEAMCKPR S+ HLL+QL
Sbjct: 498 KDVDLCPGIGLTMPKAIPLEAMCKPRASVTHLLSQL 522

BLAST of MELO3C016793.2 vs. TrEMBL
Match: tr|F6GZW4|F6GZW4_VITVI (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_18s0001g09660 PE=3 SV=1)

HSP 1 Score: 586.6 bits (1511), Expect = 5.5e-164
Identity = 298/514 (57.98%), Postives = 387/514 (75.29%), Query Frame = 0

Query: 25  TWFS-LSLLLITTLILLSKL--RKVRNQKKLPPTPPSLPIIGHLHLLKEPFHRVLHNLSN 84
           TWF  LS+     LI+LSKL   K    +KLPP+PPS PIIGHLHLLKEP HR L +LS+
Sbjct: 120 TWFRFLSVSSSLFLIVLSKLFYHKQSRGRKLPPSPPSFPIIGHLHLLKEPVHRSLQHLSD 179

Query: 85  KYGPILSLTIGSRPVLVVSSPTAVRECFTKNDIVFANRPRLISGKYINYNYTAVGFAPYG 144
           +YGP+L+L  G R VL++SSP+AV ECFTK+D VFANRPRL++GK+++Y+YT +G APYG
Sbjct: 180 QYGPVLTLQFGFRTVLLLSSPSAVEECFTKHDQVFANRPRLLAGKHLHYDYTTLGVAPYG 239

Query: 145 QHWRNMRRIATTELLSNHRLNMYLNIRVEELKLWVKNNLYKWGSGGDFVVVDMKSKLKEL 204
           QHWRN+RR+ T E+ S +RLNM+L IR +E+K  ++N   + G G  F  V+MKS+L EL
Sbjct: 240 QHWRNLRRLTTLEIFSTNRLNMFLGIRQDEVKFLLRNLFQRSGQG--FARVEMKSRLSEL 299

Query: 205 SFNTVMRMISGKRYFGVEVEDVEEAFEFREIMKELLELSGATNAADFLKILRVFDLEGVK 264
           SFN + R+++GKRYFG +VED EEA  FR+I+KE+LE SGA+NA DFL  L+ FD +G+K
Sbjct: 300 SFNIITRIVAGKRYFGTDVEDFEEASRFRDIIKEILETSGASNAGDFLPFLQWFDFQGLK 359

Query: 265 KRMMKASGRADVFLQKLIDEERKKKRVSRWPEEKQKQAKT-SMIRTLLSLQESQPQYYSD 324
           KR++    R D FLQ LIDE R      R     Q++ KT + + ++L+LQ S+P+YY+D
Sbjct: 360 KRVLALQRRTDAFLQGLIDEGRNHNNSYR-----QERGKTKTFVDSMLALQNSEPEYYAD 419

Query: 325 DIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPMTMKKAWIEIRDCVGENQMVEEGDVSE 384
            IIKG +LT+L AGTDT++ T+EWAMSLLLNHP  + K   E+   +G  ++VEE D+S+
Sbjct: 420 HIIKGMILTLLTAGTDTSAVTMEWAMSLLLNHPTVLDKVKTELDCKIGHQRLVEEPDLSD 479

Query: 385 LKYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFEVPKGTMLLVNAWGIHRDPKVWEDP 444
           L YL AI+ ETLR+FPAAPLLV HE S+DCSI G++V  GTMLLVNAW IHRD KVWEDP
Sbjct: 480 LPYLRAIVNETLRLFPAAPLLVAHESSDDCSIGGYDVRGGTMLLVNAWAIHRDAKVWEDP 539

Query: 445 TSFQPERFLNWEGVETYKWIPFGMGRRACPGVALAQRSMGLTLATLIQCFEWKKVDEQEQ 504
           TSF+PERF   EG E  ++IPFG+GRR CPG  LA R MGL LA L+QCFEW++V E E 
Sbjct: 540 TSFRPERFEGGEG-EACRFIPFGLGRRGCPGAGLANRVMGLALAALVQCFEWQRVGEVE- 599

Query: 505 IDLSEGSGITMPKAKALEAMCKPRTSMLHLLAQL 535
           +D+SEG G+TMPKA+ LEAMC+ R SM+ +L++L
Sbjct: 600 VDMSEGKGLTMPKAQPLEAMCRARNSMIKVLSEL 624

BLAST of MELO3C016793.2 vs. TrEMBL
Match: tr|A0A2R6RN81|A0A2R6RN81_ACTCH (Cytochrome P450 81E8 like OS=Actinidia chinensis var. chinensis OX=1590841 GN=CEY00_Acc04759 PE=3 SV=1)

HSP 1 Score: 573.2 bits (1476), Expect = 6.2e-160
Identity = 293/517 (56.67%), Postives = 375/517 (72.53%), Query Frame = 0

Query: 19  MEPHTITWF-SLSLLLITTLILLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKEPFHRVLH 78
           M+P+   WF  LSL    +L+LL+                 LPIIGHLHLLKEP HR LH
Sbjct: 1   MDPN---WFYFLSLATFLSLLLLNXXXXXXXXXXXXXXXXXLPIIGHLHLLKEPLHRTLH 60

Query: 79  NLSNKYGPILSLTIGSRPVLVVSSPTAVRECFTKNDIVFANRPRLISGKYINYNYTAVGF 138
            LS  +GP+L+L  GSRPVLVVSSP+A+ ECF+KND++FANRPR + GK++NYN+T +G 
Sbjct: 61  RLSASHGPVLALRFGSRPVLVVSSPSAIEECFSKNDLLFANRPRFLVGKHLNYNFTTLGA 120

Query: 139 APYGQHWRNMRRIATTELLSNHRLNMYLNIRVEELKLWVKNNLYKWGSGGDFVVVDMKSK 198
            PYG HWRN+RR +T ELLS  +LNM+L+IR EE+K  +KN      +   F  V+MKS+
Sbjct: 121 TPYGHHWRNLRRFSTVELLSTSKLNMFLSIRQEEVKSLLKNL-----NQPKFAKVEMKSR 180

Query: 199 LKELSFNTVMRMISGKRYFGVEVEDVEEAFEFREIMKELLELSGATNAADFLKILRVFDL 258
           L ELSFN VMRM+SGKRYFG E ED E A  FR I++E+ ELSGA+N  DF+  LR  D 
Sbjct: 181 LSELSFNIVMRMVSGKRYFGAEAEDSESAQRFRGIIREIFELSGASNPGDFMPFLRWIDF 240

Query: 259 EGVKKRMMKASGRADVFLQKLIDEERKKKRVSRWPEEKQKQAKTSMIRTLLSLQESQPQY 318
             +++RM+      D FLQ LIDE R K        +       SM  ++L+LQES+P+ 
Sbjct: 241 GNLERRMLNIQKEVDEFLQGLIDEIRNK--------DPNVGRNKSMTDSMLALQESEPES 300

Query: 319 YSDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPMTMKKAWIEIRDCVGENQMVEEGD 378
           YSD+IIKG++LTML+AGTDT++ TIEWAMSLLLNHP  +K+A  E+ + VGE+++ +E D
Sbjct: 301 YSDEIIKGNILTMLSAGTDTSAVTIEWAMSLLLNHPEVLKRARAELDEIVGEDRLADEPD 360

Query: 379 VSELKYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFEVPKGTMLLVNAWGIHRDPKVW 438
           +S+L YL+ II ETLR+FPAAPLLVPHE S+DC I GF+VP+GTMLL NAW IHRDPK+W
Sbjct: 361 LSKLPYLQNIINETLRLFPAAPLLVPHESSDDCFIGGFDVPRGTMLLANAWAIHRDPKLW 420

Query: 439 EDPTSFQPERFLNWEGVETYKWIPFGMGRRACPGVALAQRSMGLTLATLIQCFEWKKVDE 498
           EDPTSF PER+ N EG E YK IPFG+GRR+CPG  LA R +GL LA+LIQCFEW++V E
Sbjct: 421 EDPTSFNPERYDNREG-EAYKLIPFGIGRRSCPGAGLANRVVGLALASLIQCFEWERVSE 480

Query: 499 QEQIDLSEGSGITMPKAKALEAMCKPRTSMLHLLAQL 535
            E +D+SEG G+TMPK K LEAMC+ R  M+++L++L
Sbjct: 481 -ELVDMSEGQGLTMPKLKPLEAMCRSRERMINVLSEL 499