MELO3C015329 (gene) Melon (DHL92) v3.5.1

NameMELO3C015329
Typegene
OrganismCucumis melo (Melon (DHL92) v3.5.1)
DescriptionGlutamyl-tRNA(Gln) amidotransferase subunit A
Locationchr2 : 793725 .. 798062 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGTGGATGATTGTGGAGCTTTCATAGAGAAATTTCTCCTGCAGATGAGCTCTCCCTCCGATCAACTTCCCTTAAGTGGTCTCACTTTTGCCGTTAAAGACATGTGAGTTTCTGATTTTTTACTGTTTAAGTTCATCAAGCCTTTGGTGATCAATAGTCTGTGGGATCTGAAATATCTGTATGGAATCAAAAGATTTAATGAACAGTTCTGAAATATCGTTACTGTTATATTTGTAGATGTTGATGCTTCCATTTTTACAAGCCATCAATTGTTGGTTTTTCTATTTCATTGGAGCTTAGTTCGATAAAATAATTCTTGGTTCTTATGTTTTGAAACGACAACATGGCAGTTTTTTGTTTTCTACATGAAATTGGAGTATTAAAAGTCAAACTTCTTTTGGACTTTAAACCAAACAGATTTGATATAGAAGGGCATGTAGCTGGTTTTGGAAATCCTGAATGGCTAAGGACTCACCCACCTGCCAATCAAACGGCGCCAGCTGTGTTGACTATCCTAAGAGGAGGGGCCACCTGCATTGGCAAGACTATGATGGATGAAATGGCCTTTAGGTAACAAGAAAAATTACCCTGTACTTTATTTTTTGTCTCGTTGGCTTTCAATTTTATACCGCGTATGAAAATATTTTGGAAATGAGGATTGAACTTTCGACCTCTTTGGCACGAGGGATTGAACCTTCAACTTCTAGGGGATGGAACGATGGAATGTGTATATTGCAATTGTGGGGTTGAGGATTAATCCTTGGACCTCTAGGAAAAAGAATGCATGTCAATTGTCATGTTAAGCTTGTATTCATGGACACACAACTCATAACCTTTGGGATTCCTTAGTTGGTTTAGCTATATTATTACCATTCTTTTTCAAGCAGACAATTCTGTCTTCAAATTGGTTAAGACATGACATCAACGTGAACTTGTCAGATTTCTAATTTGTAATTGAAATGTTTGACCAATTGGTTCATCCCAATTCCTAACATACACACAGGCTTTTGAACGAATATTAATCACTACTTTGATAATCATATTTTTGGCAGTATAAATGGGGAAAACTTTCACTATGGCACACCCCAAAACCCATGTGCATCAGGTCGGATACCTGGAGGATCTTCCAGTGGTTCTGGTGTTGCTGTTGCTGCAAAGCTTGTGGATTTCTCCTTAGGCGTGATATTTCTTCTAAGTCCTTGAATAACTTGTGATTCCATGCCTTGAACTTTGTAAATTATTGTAAAAGACCATACCTCAATTTGTAGGAACCGATACCGGAGGTAGTGTAAGAGTGCCTGCATCCTACTGTGGAGTTCTCGGATTCCGGCCTTCACATGGTGTGGTCTCTACATCTGGAGTAATACCAATGACACAGAGCTTTGATACAGTAGGTAATGTGTAATCTCTCATTGTCACATTGTCTACATCGTCTTTTGTAGTAGATAAGCACAATAGGCTGATTTTTTCTCCGAATTTTAAGTCAACTCTTGATCTCCTTTGTGAAACTGTCTATTTTTTTCAACTAAGGCCCTTTGGTGCTGTCATGCAAAGTTGTTTCCTTTTTTCTTTTTTAACTTTTACTTTTTAATTTTATAGGTAAAAGGTTTTTTGAAAAACATTTATGATTATTTTTTTTAAACAGAACCTGAAGTTTGGAAATACAGTACTCCATAATTTCCATTATAATCATGACATCATTGTGATGATGATGTCTATGCTATTCATGATTTTTCATATAGATGAGCAAAGTGATGTAGATTCGTAGCAGAGCCATTGGCATCTCCTCTTTCCTTCGATATCAACTATGTTTCGCTAGCTATCCTGCCATGTGAAAATGGTGTAGATTCCACTGACACCATCTTCTCGTTTTAGGTTGGTTCGCCAGAGATTCTGCCATATTAAAGAGAGTAGGTTGGCTGCTGCTGCAAGAGCCAGAGGTTGAGCATTACAAGCCTACGAAGGTGTTCATTGCAGAAGATTGTTTCAAGCTCCTCTCAAGCATTACTAGTGAACGGTTGACACAAGCTTTTGTTAGTTCAGTTGAGAAGTTATTTGGTGGTACGTGTAAGCTGACATTCTTGTATTAGTGATTTTGTTTTTTAAAATTAGGTACCCTTTCATTTAGGAAGAATAAAGAAAGAGAGGCGTACAAAAAGTTGTTCACGCTATGAACTTCAATCAAGCATAATAAGACTACCCAAAGTGTAATTACAAAATCTTTCTAACACCAAGGCTGGAAGAGAGGTATCAAACCTAACAAGGGACTAAAAACTTCTCCCCCAGAACTTGCAAACCTCTTAACATCAAGCCAAATGGGTTATGATGTTTGTATTATGTTGTTTCCTTGTGCATATGTTTGTGTTTGTGCTTCTTAGATACCTAATATACTGGATCGTTTGAAAGAAAAAATTGGAAAAGTACCTTTTTAGTTAGCTATGAAGCATATGTGTGTTTGGTTCGTAAGTTTTCAAAATATACTTTTTTAGTTACTAAGGATTTTGGAATTGGTGCATTTGGTCCCTGAGTTTTCAACTATACCTTTTTATTGTCCAAATAGTTTTAAAAGGTCCTTGAAGTATTTTTTAGTTCTACAATAATTATATGACATTTTGAATTACGAAATAAGCGTAAAAAAATAAAAAATAATTTTATGGAGAAGATGTAAATTTTTAAAAAATCATTTTTCTAACTTAAAAGACCAAAAAGTTAAAAAAAAAGAATACTGCGGGCACTTTCTAAACCTATATTTAAAAACTCGGGGATTAAAAAGGTGTATAAAACTTAGGGACCAAATGCATATACTCCAAAGAACTTGGGGACTAAAAAAGGTATGTTTGGAAACATAGAGACTAAACACATAATTCTTCAAGACTTGGGACTGTAAAGGTAATTCTTTTAAAAAAGATTACTAATTTGATATTGGAGTTCATCTTTTAGATATAAAGATTTCAATTGGTATAGGTGATTACTTATTTGACCTTTCAACTGCAGGCCATCTTATTAAACAAATTAGCCTTGGAAAGTATGTCGAGGATAAGGTTCCAAGTTTGAAGCATTTCATGATTGAAGAAAATGATGGATATAAGCATAGCATACCATCCTTGGCAGCCCTTGTAAGATCTAAGCGATTGCTTCAGAGGTTTTGATCTTGTGATGAACCGCAACTCAAATTCTGTCGAGCTAATTGATTCTGATATATAATTGTGTTGAATGAATGAATGATCTTTGCCCTTGTCTTTGCTGCCCACTTGCAGGCATGAATTTAAAATCAATCACGGAGAATGGGTTGGGTCCTACAGTTCTCATTTGGGTCCAGGAATATCAGAACAAATATTAGAATTCATCAGGGAGGCAACAGATGAAAATATTGATCTGAGTCGCTCCATTCAAATCGAACTGCGCGAAGTTCTTGCTGCTCTTCTTGAAGTATTATGTTTTTCCCACACACACAATTTACTAAAATGTTAAACTAATATAATCAGTATCTTAAAAATGTGTGCATTTTGATATGTACTCAACTTTCATTGCTTTTTTGTGTAGAAAGAGAAAAGCTATAACCATTGTAGTTTCCATTTGATATTTCAACATAATTATAGTGTCATTGGTCATTTTATTACTGATTGGATAGTTGCTATTGGATCATCTACTTTGGAATAACGCAATGTTTCTTTTAGAAAAAAGGTTTACTTGTATGATTACACTGTGTCATCTGACAAACATATCAAATTTCTTTGAAATTTATTTCTAGAACTATAATGGGACATTGGTGTTTGTTTCCCGAGAATATTTATTCTATTCATTCTATCTCAAAAATCTATTATTAGACATTTTACTATAGAAATAAGCAGTTTGTGAATTTAAAAAAAATCATGAGTTGCTATTGTTTATCATTTCGTTTCAGGATTTTGGAGTACTTGCTATTCCTACAGTCCCAGGCCCTCCTCCAAAACTAAACACAGACATCTCGGAGCAATTCGACTTTCGTGCGAAGGCTTTCAGCTTGCTCACCATTGCTGCTGTCTCTGGAGTCTGCCAGGTTCTCTCTCTCTTGAAAGTTTTTTTGTATTTTTTTTAAGTACAAGTGGGGATGAGAGATTTGAACCATGGACCTCTTGGTTCTATTGGCGTCACTTACTTGAAAGTTAAAAAGGATGTATTTTTTTTTTTCTCACCAACTAATTAATCTGCAGGTTAGCATACCTCTAGGGTTGTACAATGGTCTTCCTGTATCAATATCTTTGCTAGCAAAACATGGCTCAGATGGATTTTTGCTCAATCTTGTTGATTGTCTTTACAGCACTCTAAAAGAAGAAGTTGAGGCAATCTACTAA

mRNA sequence

ATGGCTGTGGATGATTGTGGAGCTTTCATAGAGAAATTTCTCCTGCAGATGAGCTCTCCCTCCGATCAACTTCCCTTAAGTGGTCTCACTTTTGCCGTTAAAGACATATTTGATATAGAAGGGCATGTAGCTGGTTTTGGAAATCCTGAATGGCTAAGGACTCACCCACCTGCCAATCAAACGGCGCCAGCTGTGTTGACTATCCTAAGAGGAGGGGCCACCTGCATTGGCAAGACTATGATGGATGAAATGGCCTTTAGTATAAATGGGGAAAACTTTCACTATGGCACACCCCAAAACCCATGTGCATCAGGTCGGATACCTGGAGGATCTTCCAGTGGTTCTGGTGTTGCTGTTGCTGCAAAGCTTGTGGATTTCTCCTTAGGAACCGATACCGGAGGTAGTGTAAGAGTGCCTGCATCCTACTGTGGAGTTCTCGGATTCCGGCCTTCACATGGTGTGGTCTCTACATCTGGAGTAATACCAATGACACAGAGCTTTGATACAGTAGGTTGGTTCGCCAGAGATTCTGCCATATTAAAGAGAGTAGGTTGGCTGCTGCTGCAAGAGCCAGAGGTTGAGCATTACAAGCCTACGAAGGTGTTCATTGCAGAAGATTGTTTCAAGCTCCTCTCAAGCATTACTAGTGAACGGTTGACACAAGCTTTTGTTAGTTCAGTTGAGAAGTTATTTGGTGGCCATCTTATTAAACAAATTAGCCTTGGAAAGTATGTCGAGGATAAGGTTCCAAGTTTGAAGCATTTCATGATTGAAGAAAATGATGGATATAAGCATAGCATACCATCCTTGGCAGCCCTTGTAAGATCTAAGCGATTGCTTCAGAGGCATGAATTTAAAATCAATCACGGAGAATGGGTTGGGTCCTACAGTTCTCATTTGGGTCCAGGAATATCAGAACAAATATTAGAATTCATCAGGGAGGCAACAGATGAAAATATTGATCTGAGTCGCTCCATTCAAATCGAACTGCGCGAAGTTCTTGCTGCTCTTCTTGAAGATTTTGGAGTACTTGCTATTCCTACAGTCCCAGGCCCTCCTCCAAAACTAAACACAGACATCTCGGAGCAATTCGACTTTCGTGCGAAGGCTTTCAGCTTGCTCACCATTGCTGCTGTCTCTGGAGTCTGCCAGGTTAGCATACCTCTAGGGTTGTACAATGGTCTTCCTGTATCAATATCTTTGCTAGCAAAACATGGCTCAGATGGATTTTTGCTCAATCTTGTTGATTGTCTTTACAGCACTCTAAAAGAAGAAGTTGAGGCAATCTACTAA

Coding sequence (CDS)

ATGGCTGTGGATGATTGTGGAGCTTTCATAGAGAAATTTCTCCTGCAGATGAGCTCTCCCTCCGATCAACTTCCCTTAAGTGGTCTCACTTTTGCCGTTAAAGACATATTTGATATAGAAGGGCATGTAGCTGGTTTTGGAAATCCTGAATGGCTAAGGACTCACCCACCTGCCAATCAAACGGCGCCAGCTGTGTTGACTATCCTAAGAGGAGGGGCCACCTGCATTGGCAAGACTATGATGGATGAAATGGCCTTTAGTATAAATGGGGAAAACTTTCACTATGGCACACCCCAAAACCCATGTGCATCAGGTCGGATACCTGGAGGATCTTCCAGTGGTTCTGGTGTTGCTGTTGCTGCAAAGCTTGTGGATTTCTCCTTAGGAACCGATACCGGAGGTAGTGTAAGAGTGCCTGCATCCTACTGTGGAGTTCTCGGATTCCGGCCTTCACATGGTGTGGTCTCTACATCTGGAGTAATACCAATGACACAGAGCTTTGATACAGTAGGTTGGTTCGCCAGAGATTCTGCCATATTAAAGAGAGTAGGTTGGCTGCTGCTGCAAGAGCCAGAGGTTGAGCATTACAAGCCTACGAAGGTGTTCATTGCAGAAGATTGTTTCAAGCTCCTCTCAAGCATTACTAGTGAACGGTTGACACAAGCTTTTGTTAGTTCAGTTGAGAAGTTATTTGGTGGCCATCTTATTAAACAAATTAGCCTTGGAAAGTATGTCGAGGATAAGGTTCCAAGTTTGAAGCATTTCATGATTGAAGAAAATGATGGATATAAGCATAGCATACCATCCTTGGCAGCCCTTGTAAGATCTAAGCGATTGCTTCAGAGGCATGAATTTAAAATCAATCACGGAGAATGGGTTGGGTCCTACAGTTCTCATTTGGGTCCAGGAATATCAGAACAAATATTAGAATTCATCAGGGAGGCAACAGATGAAAATATTGATCTGAGTCGCTCCATTCAAATCGAACTGCGCGAAGTTCTTGCTGCTCTTCTTGAAGATTTTGGAGTACTTGCTATTCCTACAGTCCCAGGCCCTCCTCCAAAACTAAACACAGACATCTCGGAGCAATTCGACTTTCGTGCGAAGGCTTTCAGCTTGCTCACCATTGCTGCTGTCTCTGGAGTCTGCCAGGTTAGCATACCTCTAGGGTTGTACAATGGTCTTCCTGTATCAATATCTTTGCTAGCAAAACATGGCTCAGATGGATTTTTGCTCAATCTTGTTGATTGTCTTTACAGCACTCTAAAAGAAGAAGTTGAGGCAATCTACTAA

Protein sequence

MAVDDCGAFIEKFLLQMSSPSDQLPLSGLTFAVKDIFDIEGHVAGFGNPEWLRTHPPANQTAPAVLTILRGGATCIGKTMMDEMAFSINGENFHYGTPQNPCASGRIPGGSSSGSGVAVAAKLVDFSLGTDTGGSVRVPASYCGVLGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAILKRVGWLLLQEPEVEHYKPTKVFIAEDCFKLLSSITSERLTQAFVSSVEKLFGGHLIKQISLGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRLLQRHEFKINHGEWVGSYSSHLGPGISEQILEFIREATDENIDLSRSIQIELREVLAALLEDFGVLAIPTVPGPPPKLNTDISEQFDFRAKAFSLLTIAAVSGVCQVSIPLGLYNGLPVSISLLAKHGSDGFLLNLVDCLYSTLKEEVEAIY*
BLAST of MELO3C015329 vs. Swiss-Prot
Match: AMI1_ARATH (Amidase 1 OS=Arabidopsis thaliana GN=AMI1 PE=1 SV=1)

HSP 1 Score: 505.0 bits (1299), Expect = 8.2e-142
Identity = 260/419 (62.05%), Postives = 315/419 (75.18%), Query Frame = 1

Query: 4   DDCGAFIEKFLLQMSSPSDQLP-LSGLTFAVKDIFDIEGHVAGFGNPEWLRTHPPANQTA 63
           +D GAFIEK  +  +S S   P L GLTFA+KDIFD+EG V GFGNP+WLRTH  A  TA
Sbjct: 5   NDFGAFIEKVTISPTSTSSSPPSLQGLTFAIKDIFDVEGRVTGFGNPDWLRTHSAATSTA 64

Query: 64  PAVLTILRGGATCIGKTMMDEMAFSINGENFHYGTPQNPCASGRIPGGSSSGSGVAVAAK 123
           P V ++L  GAT +G T+MDEMA+SINGEN HYGTP+NP A  R+PGGSSSGS VAVAA+
Sbjct: 65  PVVSSLLEAGATALGITIMDEMAYSINGENAHYGTPRNPIAFDRVPGGSSSGSAVAVAAR 124

Query: 124 LVDFSLGTDTGGSVRVPASYCGVLGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAILKR 183
           LVDFS+GTDTGGSVRVPASYCG+ GFRPSHG VST G+ PM QSFDTVGWFARD+A LKR
Sbjct: 125 LVDFSIGTDTGGSVRVPASYCGIFGFRPSHGAVSTVGLTPMAQSFDTVGWFARDTATLKR 184

Query: 184 VGWLLLQEPEVEHYKPTKVFIAEDCFKLLSSITSERLTQAFVSSVEKLFGGH-LIKQISL 243
           VG +LLQ+  +   +P+++ IA+DCFK L S+  + L Q  V SVEK FGG+ ++K+++L
Sbjct: 185 VGCVLLQQHHLNPIEPSQLIIADDCFK-LCSVPHDLLVQPLVGSVEKSFGGNTVVKKVNL 244

Query: 244 GKYVEDKVPSLKHFMIEEN--DGYKHSIPSLAALVRSKRLLQRHEFKINHGEWVGSYSSH 303
           G+Y+   VPSLKHFM  ++     +  IPSL AL  S RLLQRHEFKINHG W+ S    
Sbjct: 245 GEYIGQNVPSLKHFMTSDDVTTQQEFCIPSLMALSSSMRLLQRHEFKINHGAWISSVKPE 304

Query: 304 LGPGISEQILEFIREATDENIDLSRSIQIELREVLAALLEDFGVLAIPTVPGPPPKLNTD 363
            GPGISE+I E IR  +DE ID  RS++ EL   L+ LL + GVL IPTVPGPPP L  +
Sbjct: 305 FGPGISERIEEAIR-TSDEKIDHCRSVKSELITALSTLLGEKGVLVIPTVPGPPPHLQAN 364

Query: 364 ISEQFDFRAKAFSLLTIAAVSGVCQVSIPLGLYNGLPVSISLLAKHGSDGFLLNLVDCL 419
           ++    FR++AFSLL+IA VSG CQVSIPLGL+  LPVS+SL+AK+GSDGFLL+LVD L
Sbjct: 365 VAALESFRSRAFSLLSIAGVSGFCQVSIPLGLHENLPVSVSLVAKYGSDGFLLSLVDSL 421

BLAST of MELO3C015329 vs. Swiss-Prot
Match: TOC64_PEA (Translocon at the outer membrane of chloroplasts 64 OS=Pisum sativum GN=TOC64 PE=1 SV=1)

HSP 1 Score: 412.1 bits (1058), Expect = 7.2e-114
Identity = 210/428 (49.07%), Postives = 287/428 (67.06%), Query Frame = 1

Query: 4   DDCGAFIEKFLL----QMSSPSDQLPLSGLTFAVKDIFDIEGHVAGFGNPEWLRTHPPAN 63
           +D GAF+ K  L      + P    PLS L FA+ DIFDIEGHV+ FG+PEW RTH PA+
Sbjct: 35  EDFGAFLLKLKLLPPPPPAPPKAPHPLSSLNFAISDIFDIEGHVSTFGHPEWARTHEPAS 94

Query: 64  QTAPAVLTILRGGATCIGKTMMDEMAFSINGENFHYGTPQNPCASGRIPGGSSSGSGVAV 123
            TA AV  ++  GATCIG T++DE+A+ I+GEN H+GTP NP    R+PGGSSSG+ VAV
Sbjct: 95  STASAVSALVESGATCIGTTVVDELAYGISGENKHFGTPTNPAVPNRVPGGSSSGAAVAV 154

Query: 124 AAKLVDFSLGTDTGGSVRVPASYCGVLGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAI 183
           AA  VDFSLG DT G VRVPA +CG+LGFRPSHG VS  G+IP++ S DTVGWFA+D  +
Sbjct: 155 AANFVDFSLGVDTSGGVRVPAGFCGILGFRPSHGAVSHVGIIPVSTSLDTVGWFAKDPDV 214

Query: 184 LKRVGWLLLQEPEVEHYKPTKVFIAEDCFKLLSSITSERLTQAFVSSVEKLFGGHLIKQI 243
           L+RVG +LLQ P V    P ++ IA+DCF+ L ++  +R +Q  + + EKLFG  ++K I
Sbjct: 215 LRRVGHILLQAPFVMQRNPRQIIIADDCFQHL-NVPLDRTSQVVIKATEKLFGKQVLKHI 274

Query: 244 SLGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRLLQRHEFKINHGEWVGSYSSH 303
           +   Y+  KV SLK   I++++G   S  SL  L    + LQRHEF+  H EW+      
Sbjct: 275 NFEDYISSKVSSLKACSIQKSNGVLKS-SSLKLLANVMQSLQRHEFEHTHSEWMSIVKPD 334

Query: 304 LGPGISEQILEFIREATDENIDLSRSIQIELREVLAALLEDFGVLAIPTVPGPPPKLNTD 363
           L P +S Q+ E   E ++  I+ S+S++ ELR  + +LL+D GVL IPTV  PPPKL   
Sbjct: 335 LHPAVSAQLHEKF-EVSELEIENSKSVRSELRVAVNSLLKDEGVLVIPTVADPPPKLGGK 394

Query: 364 ISEQFDFRAKAFSLLTIAAVSGVCQVSIPLGLYNGLPVSISLLAKHGSDGFLLNLVDCLY 423
                D++++A SLL+IA++SG CQV++PLG ++  PVS+SL+A+HG D FLL+ +  +Y
Sbjct: 395 EFLSHDYQSRALSLLSIASISGCCQVTVPLGFFDKNPVSVSLIARHGGDRFLLDTLKTMY 454

Query: 424 STLKEEVE 428
           + L+E+ +
Sbjct: 455 TVLQEQAD 459

BLAST of MELO3C015329 vs. Swiss-Prot
Match: OE64C_ARATH (Outer envelope protein 64, chloroplastic OS=Arabidopsis thaliana GN=OEP64 PE=1 SV=1)

HSP 1 Score: 401.4 bits (1030), Expect = 1.3e-110
Identity = 205/425 (48.24%), Postives = 280/425 (65.88%), Query Frame = 1

Query: 4   DDCGAFIEKFLL----QMSSPSDQLPLSGLTFAVKDIFDIEGHVAGFGNPEWLRTHPPAN 63
           +D GAFI+K +L    Q + P    PL+GLTFAV D+FDI G+V GFG+P+W+RTH  A+
Sbjct: 32  EDFGAFIDKLMLLPPPQPAPPKAPHPLTGLTFAVSDVFDITGYVTGFGHPDWVRTHEAAS 91

Query: 64  QTAPAVLTILRGGATCIGKTMMDEMAFSINGENFHYGTPQNPCASGRIPGGSSSGSGVAV 123
            T+P V T++ GGATC+GKT++DE AFSI+GEN HY +P NP A  RIPGG+ SG+ VAV
Sbjct: 92  STSPVVSTLVEGGATCVGKTVVDEFAFSISGENKHYDSPTNPAAPTRIPGGACSGAAVAV 151

Query: 124 AAKLVDFSLGTDTGGSVRVPASYCGVLGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAI 183
           A   VDF+LG DT G VRVPA YCGVLGF+ S+G +S +G+IP++ S D+VGWFARD   
Sbjct: 152 ATNAVDFALGIDTVGGVRVPAGYCGVLGFKSSYGAISNTGIIPVSSSLDSVGWFARDPNT 211

Query: 184 LKRVGWLLLQEPEVEHYKPTKVFIAEDCFKLLSSITSERLTQAFVSSVEKLFGGHLIKQI 243
           L+RVG +LLQ P      P ++ +A+DCF+LL  I  +R+TQ    S EKLFG  L+K  
Sbjct: 212 LRRVGHVLLQLPFATQRNPRQIILADDCFQLL-KIPVDRITQVVTKSAEKLFGRQLLKHQ 271

Query: 244 SLGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRLLQRHEFKINHGEWVGSYSSH 303
           +L  Y E KVPSLK F   +       + +   L    +LLQRHEF  NHG+W+ +    
Sbjct: 272 NLETYFETKVPSLKEFARTKAIA-NTKVSTSRLLANVMQLLQRHEFLQNHGDWINTVKPA 331

Query: 304 LGPGISEQILEFIREATDENIDLSRSIQIELREVLAALLEDFGVLAIPTVPGPPPKLNTD 363
           + P I  Q+ E   E T+E  +   +I+ E R  + +LL+D G+L IPT+P  PPKL + 
Sbjct: 332 IDPVILSQVCE-NPELTNEETENLNAIRNETRVAIGSLLKDDGILVIPTLPAVPPKLGSK 391

Query: 364 ISEQFDFRAKAFSLLTIAAVSGVCQVSIPLGLYNGLPVSISLLAKHGSDGFLLNLVDCLY 423
                D++ +A SLL+IA++SG CQV++PLG +   P+S+S + +HG D FLL+ V  +Y
Sbjct: 392 EITSEDYQNRASSLLSIASISGCCQVTVPLGHHEKCPISVSFIGRHGGDRFLLDTVQTMY 451

Query: 424 STLKE 425
            +L+E
Sbjct: 452 PSLQE 453

BLAST of MELO3C015329 vs. Swiss-Prot
Match: OE64M_ARATH (Outer envelope protein 64, mitochondrial OS=Arabidopsis thaliana GN=OM64 PE=1 SV=1)

HSP 1 Score: 359.4 bits (921), Expect = 5.6e-98
Identity = 188/429 (43.82%), Postives = 275/429 (64.10%), Query Frame = 1

Query: 4   DDCGAFIEKFLL----QMSSPSDQLPLSGLTFAVKDIFDIEGHVAGFGNPEWLRTHPPAN 63
           +D GAF+++F L        P+ +  LSGLTF++ D FD++ ++ GFG P+W +TH  A 
Sbjct: 45  EDFGAFLDRFELLPFPPPPPPAAKQSLSGLTFSISDAFDVKDYITGFGCPQWKKTHEAAE 104

Query: 64  QTAPAVLTILRGGATCIGKTMMDEMAFSINGENFHYGTPQNPCASGRIPGGSSSGSGVAV 123
           +TA  V T+L+ GATC+GKT+MDE+ F I GEN HYGTP NP     +PGG SSGS V+V
Sbjct: 105 KTAVVVTTLLKNGATCVGKTIMDELGFGIIGENKHYGTPINPLMPDNVPGGCSSGSAVSV 164

Query: 124 AAKLVDFSLGTDTGGSVRVPASYCGVLGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAI 183
            A+LVDFSLG DT G VRVPA++CG+LGFRPS G VS+ GV+P +QS +TVGWFA D ++
Sbjct: 165 GAELVDFSLGIDTTGGVRVPAAFCGILGFRPSQGTVSSVGVLPNSQSLETVGWFASDPSV 224

Query: 184 LKRVGWLLLQEPEVEHYKPTKVFIAEDCFKLLSSITSERLTQAFVSSVEKLFGGHLIKQI 243
           L +VG  LL    V H +   +  A+D F+ LS I  ++  Q    ++E L G    K +
Sbjct: 225 LCQVGHALLNLSAVTHRRQRSLIFADDLFE-LSDIPKQKSVQVVRKAIENLSGYKTPKHV 284

Query: 244 SLGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRLLQRHEFKINHGEWVGSYSSH 303
           ++G+YV   VPSL  F  E++   ++S  +L AL      +QRHEFK NH EW  +  S 
Sbjct: 285 NVGQYVASNVPSLAEF-CEQSGKSQNSASTLRALSSVMLAIQRHEFKTNHEEWWQTCKSF 344

Query: 304 LGPGISEQILEFIREATDENIDLSRSIQIELREVLAALLEDFGVLAIPTVPGPPPKLNTD 363
           LGP  S  ++  ++ + +E+I     ++ E+R  + +LL++ G+L IPTV  PPP+LNT 
Sbjct: 345 LGPRFSNDVVTALK-SKNESIKSLYRVKNEMRATIQSLLKEDGILVIPTVADPPPRLNTK 404

Query: 364 ISEQF-DFRAKAFSLLTIAAVSGVCQVSIPLGLYNGLPVSISLLAKHGSDGFLLNLVDCL 423
            ++   +F  + ++L  IA++SG CQV+IPLG +   P+S+SLL  +G D FLL+    +
Sbjct: 405 RNKSLNEFLDRTYALSCIASMSGCCQVTIPLGEHGDRPISVSLLTYYGGDKFLLDTTLDV 464

Query: 424 YSTLKEEVE 428
           Y++L+++ +
Sbjct: 465 YASLQDQAK 470

BLAST of MELO3C015329 vs. Swiss-Prot
Match: GATA_SULTO (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Sulfolobus tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) GN=gatA PE=3 SV=2)

HSP 1 Score: 152.9 bits (385), Expect = 7.9e-36
Identity = 117/410 (28.54%), Postives = 205/410 (50.00%), Query Frame = 1

Query: 26  LSGLTFAVKDIFDIEGHVAGFGNPEWLRTHPPANQTAPAVLTILRGGATCIGKTMMDEMA 85
           LSG+  A+KD    +G      +       PP + T   +  + + GA  +GKT MDE A
Sbjct: 61  LSGILIAIKDNISTKGIRTTCASRMLEDYVPPYDATV--IEKLKQEGAVILGKTNMDEFA 120

Query: 86  FSINGENFHYGTPQNPCASGRIPGGSSSGSGVAVAAKLVDFSLGTDTGGSVRVPASYCGV 145
                E  ++G  +NP    R PGGSS GSG A+AA +VD +LG+DTGGS+R PA+Y  V
Sbjct: 121 MGSTTETSYFGPTRNPWDLERTPGGSSGGSGAALAAGIVDIALGSDTGGSIRAPAAYNAV 180

Query: 146 LGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAILKRVGWLLL--------------QEP 205
            G +PS+G VS  G++    S + +G  A+++  L  +  ++               Q P
Sbjct: 181 FGLKPSYGTVSRFGLVAYANSLEQIGPMAKNAEDLALLYSIISGDDPKDATTIHFEPQVP 240

Query: 206 EVEHYKPTKVFIAEDCFKLLSSITSERLTQAFVSSVEKLFG-GHLIKQISLGKYVEDKVP 265
           E    K  K+ + +D    +   + + +   F S+++KL   G +IK+++LG Y E  +P
Sbjct: 241 EKVELKDVKIAVLKD----IVEASDKPVVSIFNSTLDKLSSEGAIIKEVNLG-YAEYALP 300

Query: 266 SLKHFMIEENDGYKHSIPSLAALVRSKRLLQRHEFKINHGEWVGSYSSHLGPG----ISE 325
           +  +++I  ++   +       L R   +   +  K + G W  +++ + G G    +  
Sbjct: 301 A--YYIIAMSEASSN-------LARFDGVRYGYS-KYSEGNWRETFAKNRGEGFGIEVKR 360

Query: 326 QIL--EFIREA--TDENIDLSRSIQIELREVLAALLEDFGVLAIPTVPGPPPKLNTDISE 385
           +IL   FI  A   +E    +  I+  +++ +  +L++F ++A PT+P  PPK+   + +
Sbjct: 361 RILLGSFILSAGYYEEFYIKALKIRRLIKDNVDNILKEFDLIASPTMPILPPKIGEVVED 420

Query: 386 QFDFRAKAFSLLTIAAVSGVCQVSIPLGLYNGLPVSISLLAKHGSDGFLL 413
                A   + + IA ++GV  +S P G YN LP+ + L+ ++ SD +++
Sbjct: 421 PIKMYAMDLNTV-IANLAGVPALSQPAGFYNNLPIGLQLMGRYLSDYYIM 452

BLAST of MELO3C015329 vs. TrEMBL
Match: A0A0A0LZ24_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G570120 PE=4 SV=1)

HSP 1 Score: 820.5 bits (2118), Expect = 9.8e-235
Identity = 410/430 (95.35%), Postives = 420/430 (97.67%), Query Frame = 1

Query: 1   MAVDDCGAFIEKFLLQMSSPSDQLPLSGLTFAVKDIFDIEGHVAGFGNPEWLRTHPPANQ 60
           MAVDD GAFIEKFLLQMSSPSD+LPLSGLTFAVKDIFDIEG+VAGFGNPEWLRTHPPANQ
Sbjct: 1   MAVDDYGAFIEKFLLQMSSPSDKLPLSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPANQ 60

Query: 61  TAPAVLTILRGGATCIGKTMMDEMAFSINGENFHYGTPQNPCASGRIPGGSSSGSGVAVA 120
           TAPAVLTILRGGATCIGKT+MDEMAFSINGENFHYGTPQNP AS RIPGGSSSGSGVAVA
Sbjct: 61  TAPAVLTILRGGATCIGKTIMDEMAFSINGENFHYGTPQNPHASDRIPGGSSSGSGVAVA 120

Query: 121 AKLVDFSLGTDTGGSVRVPASYCGVLGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAIL 180
           AKLVDFSLGTDTGGSVRVPASYCG+LGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAIL
Sbjct: 121 AKLVDFSLGTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAIL 180

Query: 181 KRVGWLLLQEPEVEHYKPTKVFIAEDCFKLLSSITSERLTQAFVSSVEKLFGGHLIKQIS 240
           KRVGWLLLQEPE+EHYKPTKVFIAEDCFKLLS+ITSERLTQAFVSSVEKLFGGHLIKQIS
Sbjct: 181 KRVGWLLLQEPEIEHYKPTKVFIAEDCFKLLSNITSERLTQAFVSSVEKLFGGHLIKQIS 240

Query: 241 LGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRLLQRHEFKINHGEWVGSYSSHL 300
           LGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKR LQRHEF INHGEWV SY SHL
Sbjct: 241 LGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRFLQRHEFNINHGEWVRSYHSHL 300

Query: 301 GPGISEQILEFIREATDENIDLSRSIQIELREVLAALLEDFGVLAIPTVPGPPPKLNTDI 360
           GPGISE+ILEFIRE TDENIDLSRSIQIELRE LAALLEDFGVLAIPTVPGPPPKLNTDI
Sbjct: 301 GPGISERILEFIREVTDENIDLSRSIQIELREALAALLEDFGVLAIPTVPGPPPKLNTDI 360

Query: 361 SEQFDFRAKAFSLLTIAAVSGVCQVSIPLGLYNGLPVSISLLAKHGSDGFLLNLVDCLYS 420
           SEQ+DFRAKAFSLLTIAAVSGVCQVS+PLGLYNGLPVSISLLAKHG+DGFLLNLVD LYS
Sbjct: 361 SEQYDFRAKAFSLLTIAAVSGVCQVSVPLGLYNGLPVSISLLAKHGADGFLLNLVDSLYS 420

Query: 421 TLKEEVEAIY 431
           TLKEEVEAIY
Sbjct: 421 TLKEEVEAIY 430

BLAST of MELO3C015329 vs. TrEMBL
Match: A0A0A0M1K1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G570110 PE=4 SV=1)

HSP 1 Score: 697.6 bits (1799), Expect = 9.6e-198
Identity = 347/430 (80.70%), Postives = 384/430 (89.30%), Query Frame = 1

Query: 1   MAVDDCGAFIEKFLLQMSSPSDQLPLSGLTFAVKDIFDIEGHVAGFGNPEWLRTHPPANQ 60
           MAV   GAF+EKFLLQ SSPSDQLPL+GLTFAVKDIFD++GHV GFGNPEWLRTHPPAN 
Sbjct: 1   MAVQCYGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANH 60

Query: 61  TAPAVLTILRGGATCIGKTMMDEMAFSINGENFHYGTPQNPCASGRIPGGSSSGSGVAVA 120
           TAP V TILRGGATCIG+T+MDEMA+SINGENFHYGTPQNPCAS R+PGGSSSGS VAV 
Sbjct: 61  TAPTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVG 120

Query: 121 AKLVDFSLGTDTGGSVRVPASYCGVLGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAIL 180
           A LVDFSLGTDTGGSVRVPASYCG+LGFRPSHG VSTSGV+PM QS DTVGWFARD  +L
Sbjct: 121 ANLVDFSLGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSMDTVGWFARDPVVL 180

Query: 181 KRVGWLLLQEPEVEHYKPTKVFIAEDCFKLLSSITSERLTQAFVSSVEKLFGGHLIKQIS 240
           K+VGWLLLQ+PEVEH KP +V IAEDCFK LSSI SERLTQAFV+SV+KLFGGH IK++S
Sbjct: 181 KKVGWLLLQQPEVEHQKPRQVLIAEDCFK-LSSIPSERLTQAFVNSVKKLFGGHPIKEVS 240

Query: 241 LGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRLLQRHEFKINHGEWVGSYSSHL 300
           LG YVE+KVPSLKHFMIE N G +HSIPSLAAL RS +LLQR+EFKINH EWV +Y+ HL
Sbjct: 241 LGNYVEEKVPSLKHFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHL 300

Query: 301 GPGISEQILEFIREATDENIDLSRSIQIELREVLAALLEDFGVLAIPTVPGPPPKLNTDI 360
           GPGISE++ E +R ATDENIDL  SI+IELR+ LAALLEDFGVLAIPTVPGPPPKLNTD+
Sbjct: 301 GPGISERVSEAMR-ATDENIDLCHSIRIELRKALAALLEDFGVLAIPTVPGPPPKLNTDV 360

Query: 361 SEQFDFRAKAFSLLTIAAVSGVCQVSIPLGLYNGLPVSISLLAKHGSDGFLLNLVDCLYS 420
           SE  DFRAKAFSLL+IA VSG CQVSIPLGLYNGLPVSISL+A HGSDGFLLN+V  LY+
Sbjct: 361 SELQDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLNVVHSLYN 420

Query: 421 TLKEEVEAIY 431
           TL+EEV+A +
Sbjct: 421 TLEEEVKASF 428

BLAST of MELO3C015329 vs. TrEMBL
Match: B9RF45_RICCO (Amidase, putative OS=Ricinus communis GN=RCOM_1431340 PE=4 SV=1)

HSP 1 Score: 575.5 bits (1482), Expect = 5.5e-161
Identity = 287/422 (68.01%), Postives = 343/422 (81.28%), Query Frame = 1

Query: 5   DCGAFIEKFLLQMSSPSDQLPLSGLTFAVKDIFDIEGHVAGFGNPEWLRTHPPANQTAPA 64
           D GAF+EKF+L+ SS SDQLPL  LTFAVKDIFD++G+V GFGNP+W RTH  A  TAPA
Sbjct: 78  DYGAFMEKFVLKPSSSSDQLPLHSLTFAVKDIFDVDGYVTGFGNPDWARTHSAATATAPA 137

Query: 65  VLTILRGGATCIGKTMMDEMAFSINGENFHYGTPQNPCASGRIPGGSSSGSGVAVAAKLV 124
           VL +LRGGATC+GK +MDEMA+SINGEN HYGTP NP A  R+PGGSSSGS VAV AKLV
Sbjct: 138 VLAVLRGGATCVGKVIMDEMAYSINGENAHYGTPTNPSAPDRVPGGSSSGSAVAVGAKLV 197

Query: 125 DFSLGTDTGGSVRVPASYCGVLGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAILKRVG 184
           DFSLG+DTGGSVRVPASYCG+LGFRPSHGVVS SGVIPM QSFDTVGWF+R+  ILK VG
Sbjct: 198 DFSLGSDTGGSVRVPASYCGILGFRPSHGVVSVSGVIPMAQSFDTVGWFSRNPVILKHVG 257

Query: 185 WLLLQEPEVEHYKPTKVFIAEDCFKLLSSITSERLTQAFVSSVEKLFGGHLIKQISLGKY 244
            LLLQ P+V+  KP ++FIAEDCF+ LSSI  +R++Q  V SVEKLFGG ++K ++LG Y
Sbjct: 258 RLLLQLPDVDRTKPNQIFIAEDCFQ-LSSIPKKRVSQVLVKSVEKLFGGDIVKHVNLGDY 317

Query: 245 VEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRLLQRHEFKINHGEWVGSYSSHLGPGI 304
           VEDKVPSL+HFM +E    +++I  LAAL  + R+LQR+EFK NHGEWV +     GPG+
Sbjct: 318 VEDKVPSLRHFMSKEIKEQEYNIAPLAALSSAMRMLQRYEFKTNHGEWVTTVKPDFGPGL 377

Query: 305 SEQILEFIREATDENIDLSRSIQIELREVLAALLEDFGVLAIPTVPGPPPKLNTDISEQF 364
            E+I E +R +T+ENID  +S++ ELR  L  LLE+FG++AIPTVPG PPKLNTD +   
Sbjct: 378 KERIWEAVR-STEENIDAFQSVRTELRAALTNLLEEFGIIAIPTVPGVPPKLNTDPTTLE 437

Query: 365 DFRAKAFSLLTIAAVSGVCQVSIPLGLYNGLPVSISLLAKHGSDGFLLNLVDCLYSTLKE 424
            FRAKAFSLL+I  VSG CQVSIPLG+++GLPVSISLLAKHGSD FLLN+V+ LY TL+E
Sbjct: 438 IFRAKAFSLLSITGVSGFCQVSIPLGMHDGLPVSISLLAKHGSDVFLLNVVETLYDTLQE 497

Query: 425 EV 427
           EV
Sbjct: 498 EV 497

BLAST of MELO3C015329 vs. TrEMBL
Match: V4T529_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10001213mg PE=4 SV=1)

HSP 1 Score: 566.6 bits (1459), Expect = 2.6e-158
Identity = 287/424 (67.69%), Postives = 341/424 (80.42%), Query Frame = 1

Query: 5   DCGAFIEKFLLQMSSPSDQLPLSGLTFAVKDIFDIEGHVAGFGNPEWLRTHPPANQTAPA 64
           D GAF+EKF+LQ SS + QLPLSGLTFA+KDIFD++GHV GFGNP+W RTH  A  TAPA
Sbjct: 7   DYGAFMEKFVLQPSSSAHQLPLSGLTFAIKDIFDVDGHVTGFGNPDWARTHSAATSTAPA 66

Query: 65  VLTILRGGATCIGKTMMDEMAFSINGENFHYGTPQNPCASGRIPGGSSSGSGVAVAAKLV 124
           VL +L GGAT IGKT+MDEMA+SINGEN HYGTP NPCA  R+PGGSSSGS VAV AKLV
Sbjct: 67  VLAVLSGGATSIGKTIMDEMAYSINGENKHYGTPTNPCAPDRVPGGSSSGSAVAVGAKLV 126

Query: 125 DFSLGTDTGGSVRVPASYCGVLGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAILKRVG 184
           DFSLGTDTGGSVRVPASYCG+ GFRPSH  VST+GVIPM QSFDTVGWFA D  IL RVG
Sbjct: 127 DFSLGTDTGGSVRVPASYCGIFGFRPSHSAVSTAGVIPMAQSFDTVGWFAWDPKILNRVG 186

Query: 185 WLLLQ-EPEVEHYKPTKVFIAEDCFKLLSSITSERLTQAFVSSVEKLFGGHLIKQISLGK 244
            +LLQ   +V   +P++V  AEDC + LSSI S+R+TQ  V SVEKLFGGH+I+ + LG 
Sbjct: 187 RVLLQLADDVNLVRPSQVIFAEDCLQ-LSSIPSDRVTQGLVKSVEKLFGGHIIENVILGD 246

Query: 245 YVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRLLQRHEFKINHGEWVGSYSSHLGPG 304
           YV+DKVPSL+HF+ E N   ++SIPSLAAL  + RLLQR+EFK NHG+WV +    LGPG
Sbjct: 247 YVKDKVPSLQHFLSEGNKEQEYSIPSLAALSSAMRLLQRYEFKNNHGDWVTTVKPDLGPG 306

Query: 305 ISEQILEFIREATDENIDLSRSIQIELREVLAALLEDFGVLAIPTVPGPPPKLNTDISEQ 364
           ISE++ E +R +  + ID+ +S++ ELR  L+ALL D G+L IPTVPGPPPKL  D S  
Sbjct: 307 ISERVWEAVRTSA-QKIDVCQSVKTELRAALSALLGDHGILVIPTVPGPPPKLQMDPSAL 366

Query: 365 FDFRAKAFSLLTIAAVSGVCQVSIPLGLYNGLPVSISLLAKHGSDGFLLNLVDCLYSTLK 424
             FRA+AFSLL+IA VSG CQVSIPLGL++ LPV+ISLLAKHGSDGFLLNLV+ L++TLK
Sbjct: 367 EVFRARAFSLLSIAGVSGFCQVSIPLGLHDNLPVAISLLAKHGSDGFLLNLVETLHNTLK 426

Query: 425 EEVE 428
           EE++
Sbjct: 427 EELQ 428

BLAST of MELO3C015329 vs. TrEMBL
Match: A5BL97_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_015334 PE=4 SV=1)

HSP 1 Score: 563.5 bits (1451), Expect = 2.2e-157
Identity = 282/423 (66.67%), Postives = 336/423 (79.43%), Query Frame = 1

Query: 5   DCGAFIEKFLLQMSSPSDQLPLSGLTFAVKDIFDIEGHVAGFGNPEWLRTHPPANQTAPA 64
           D GAF+E+F+LQ SS S +LPL+GLTFAVKDIFD++G+V GFGNP+W RTH  A  TAP+
Sbjct: 6   DYGAFMERFILQPSSSSHELPLNGLTFAVKDIFDVDGYVTGFGNPDWARTHQAAMLTAPS 65

Query: 65  VLTILRGGATCIGKTMMDEMAFSINGENFHYGTPQNPCASGRIPGGSSSGSGVAVAAKLV 124
           VL +L+GGATC+GKT+MDEMA+SINGEN HYGTP NP A  R+PGGSSSGS VAV A LV
Sbjct: 66  VLAVLKGGATCVGKTVMDEMAYSINGENKHYGTPTNPQAPDRVPGGSSSGSAVAVGAMLV 125

Query: 125 DFSLGTDTGGSVRVPASYCGVLGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAILKRVG 184
           DFSLGTDTGGSVRVPASYCG+ G RPSHGVVST+GVIPM QSFDTVGWFARD  IL RVG
Sbjct: 126 DFSLGTDTGGSVRVPASYCGIFGIRPSHGVVSTTGVIPMAQSFDTVGWFARDPEILNRVG 185

Query: 185 WLLLQEPEVEHYKPTKVFIAEDCFKLLSSITSERLTQAFVSSVEKLFGGHLIKQISLGKY 244
            +LL  P+V   KP+++ I EDCF+LL SI  +R+TQ  + SVEKLFG  ++K +SLG Y
Sbjct: 186 HVLLPFPDVNPVKPSQIIIPEDCFRLL-SIPIDRVTQVLIKSVEKLFGSDIVKHVSLGDY 245

Query: 245 VEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRLLQRHEFKINHGEWVGSYSSHLGPGI 304
           VEDKV SLK FM +EN   +++IPSLAAL  + RLLQR+EFK NHGEWV +    LGPGI
Sbjct: 246 VEDKVTSLKPFMSKENQTQEYNIPSLAALSTAMRLLQRYEFKNNHGEWVRAVQPDLGPGI 305

Query: 305 SEQILEFIREATDENIDLSRSIQIELREVLAALLEDFGVLAIPTVPGPPPKLNTDISEQF 364
            E++ E +   TDE ID+ +S++ E R  L  LL D GVL +PTVPGPPPKL TD +   
Sbjct: 306 RERVWEAL-GTTDEKIDVCQSLKTEFRSALTDLLGDSGVLVLPTVPGPPPKLQTDPTTLE 365

Query: 365 DFRAKAFSLLTIAAVSGVCQVSIPLGLYNGLPVSISLLAKHGSDGFLLNLVDCLYSTLKE 424
            FRA+AFSLL+IA VSG CQVSIPLGLY+ LPV++SLLAKHGSDGFLLNLV  LY ++KE
Sbjct: 366 SFRARAFSLLSIAGVSGFCQVSIPLGLYDNLPVAVSLLAKHGSDGFLLNLVQTLYGSIKE 425

Query: 425 EVE 428
           +VE
Sbjct: 426 QVE 426

BLAST of MELO3C015329 vs. TAIR10
Match: AT1G08980.1 (AT1G08980.1 amidase 1)

HSP 1 Score: 505.0 bits (1299), Expect = 4.6e-143
Identity = 260/419 (62.05%), Postives = 315/419 (75.18%), Query Frame = 1

Query: 4   DDCGAFIEKFLLQMSSPSDQLP-LSGLTFAVKDIFDIEGHVAGFGNPEWLRTHPPANQTA 63
           +D GAFIEK  +  +S S   P L GLTFA+KDIFD+EG V GFGNP+WLRTH  A  TA
Sbjct: 5   NDFGAFIEKVTISPTSTSSSPPSLQGLTFAIKDIFDVEGRVTGFGNPDWLRTHSAATSTA 64

Query: 64  PAVLTILRGGATCIGKTMMDEMAFSINGENFHYGTPQNPCASGRIPGGSSSGSGVAVAAK 123
           P V ++L  GAT +G T+MDEMA+SINGEN HYGTP+NP A  R+PGGSSSGS VAVAA+
Sbjct: 65  PVVSSLLEAGATALGITIMDEMAYSINGENAHYGTPRNPIAFDRVPGGSSSGSAVAVAAR 124

Query: 124 LVDFSLGTDTGGSVRVPASYCGVLGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAILKR 183
           LVDFS+GTDTGGSVRVPASYCG+ GFRPSHG VST G+ PM QSFDTVGWFARD+A LKR
Sbjct: 125 LVDFSIGTDTGGSVRVPASYCGIFGFRPSHGAVSTVGLTPMAQSFDTVGWFARDTATLKR 184

Query: 184 VGWLLLQEPEVEHYKPTKVFIAEDCFKLLSSITSERLTQAFVSSVEKLFGGH-LIKQISL 243
           VG +LLQ+  +   +P+++ IA+DCFK L S+  + L Q  V SVEK FGG+ ++K+++L
Sbjct: 185 VGCVLLQQHHLNPIEPSQLIIADDCFK-LCSVPHDLLVQPLVGSVEKSFGGNTVVKKVNL 244

Query: 244 GKYVEDKVPSLKHFMIEEN--DGYKHSIPSLAALVRSKRLLQRHEFKINHGEWVGSYSSH 303
           G+Y+   VPSLKHFM  ++     +  IPSL AL  S RLLQRHEFKINHG W+ S    
Sbjct: 245 GEYIGQNVPSLKHFMTSDDVTTQQEFCIPSLMALSSSMRLLQRHEFKINHGAWISSVKPE 304

Query: 304 LGPGISEQILEFIREATDENIDLSRSIQIELREVLAALLEDFGVLAIPTVPGPPPKLNTD 363
            GPGISE+I E IR  +DE ID  RS++ EL   L+ LL + GVL IPTVPGPPP L  +
Sbjct: 305 FGPGISERIEEAIR-TSDEKIDHCRSVKSELITALSTLLGEKGVLVIPTVPGPPPHLQAN 364

Query: 364 ISEQFDFRAKAFSLLTIAAVSGVCQVSIPLGLYNGLPVSISLLAKHGSDGFLLNLVDCL 419
           ++    FR++AFSLL+IA VSG CQVSIPLGL+  LPVS+SL+AK+GSDGFLL+LVD L
Sbjct: 365 VAALESFRSRAFSLLSIAGVSGFCQVSIPLGLHENLPVSVSLVAKYGSDGFLLSLVDSL 421

BLAST of MELO3C015329 vs. TAIR10
Match: AT3G17970.1 (AT3G17970.1 translocon at the outer membrane of chloroplasts 64-III)

HSP 1 Score: 401.4 bits (1030), Expect = 7.2e-112
Identity = 205/425 (48.24%), Postives = 280/425 (65.88%), Query Frame = 1

Query: 4   DDCGAFIEKFLL----QMSSPSDQLPLSGLTFAVKDIFDIEGHVAGFGNPEWLRTHPPAN 63
           +D GAFI+K +L    Q + P    PL+GLTFAV D+FDI G+V GFG+P+W+RTH  A+
Sbjct: 32  EDFGAFIDKLMLLPPPQPAPPKAPHPLTGLTFAVSDVFDITGYVTGFGHPDWVRTHEAAS 91

Query: 64  QTAPAVLTILRGGATCIGKTMMDEMAFSINGENFHYGTPQNPCASGRIPGGSSSGSGVAV 123
            T+P V T++ GGATC+GKT++DE AFSI+GEN HY +P NP A  RIPGG+ SG+ VAV
Sbjct: 92  STSPVVSTLVEGGATCVGKTVVDEFAFSISGENKHYDSPTNPAAPTRIPGGACSGAAVAV 151

Query: 124 AAKLVDFSLGTDTGGSVRVPASYCGVLGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAI 183
           A   VDF+LG DT G VRVPA YCGVLGF+ S+G +S +G+IP++ S D+VGWFARD   
Sbjct: 152 ATNAVDFALGIDTVGGVRVPAGYCGVLGFKSSYGAISNTGIIPVSSSLDSVGWFARDPNT 211

Query: 184 LKRVGWLLLQEPEVEHYKPTKVFIAEDCFKLLSSITSERLTQAFVSSVEKLFGGHLIKQI 243
           L+RVG +LLQ P      P ++ +A+DCF+LL  I  +R+TQ    S EKLFG  L+K  
Sbjct: 212 LRRVGHVLLQLPFATQRNPRQIILADDCFQLL-KIPVDRITQVVTKSAEKLFGRQLLKHQ 271

Query: 244 SLGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRLLQRHEFKINHGEWVGSYSSH 303
           +L  Y E KVPSLK F   +       + +   L    +LLQRHEF  NHG+W+ +    
Sbjct: 272 NLETYFETKVPSLKEFARTKAIA-NTKVSTSRLLANVMQLLQRHEFLQNHGDWINTVKPA 331

Query: 304 LGPGISEQILEFIREATDENIDLSRSIQIELREVLAALLEDFGVLAIPTVPGPPPKLNTD 363
           + P I  Q+ E   E T+E  +   +I+ E R  + +LL+D G+L IPT+P  PPKL + 
Sbjct: 332 IDPVILSQVCE-NPELTNEETENLNAIRNETRVAIGSLLKDDGILVIPTLPAVPPKLGSK 391

Query: 364 ISEQFDFRAKAFSLLTIAAVSGVCQVSIPLGLYNGLPVSISLLAKHGSDGFLLNLVDCLY 423
                D++ +A SLL+IA++SG CQV++PLG +   P+S+S + +HG D FLL+ V  +Y
Sbjct: 392 EITSEDYQNRASSLLSIASISGCCQVTVPLGHHEKCPISVSFIGRHGGDRFLLDTVQTMY 451

Query: 424 STLKE 425
            +L+E
Sbjct: 452 PSLQE 453

BLAST of MELO3C015329 vs. TAIR10
Match: AT5G09420.1 (AT5G09420.1 translocon at the outer membrane of chloroplasts 64-V)

HSP 1 Score: 359.4 bits (921), Expect = 3.1e-99
Identity = 188/429 (43.82%), Postives = 275/429 (64.10%), Query Frame = 1

Query: 4   DDCGAFIEKFLL----QMSSPSDQLPLSGLTFAVKDIFDIEGHVAGFGNPEWLRTHPPAN 63
           +D GAF+++F L        P+ +  LSGLTF++ D FD++ ++ GFG P+W +TH  A 
Sbjct: 45  EDFGAFLDRFELLPFPPPPPPAAKQSLSGLTFSISDAFDVKDYITGFGCPQWKKTHEAAE 104

Query: 64  QTAPAVLTILRGGATCIGKTMMDEMAFSINGENFHYGTPQNPCASGRIPGGSSSGSGVAV 123
           +TA  V T+L+ GATC+GKT+MDE+ F I GEN HYGTP NP     +PGG SSGS V+V
Sbjct: 105 KTAVVVTTLLKNGATCVGKTIMDELGFGIIGENKHYGTPINPLMPDNVPGGCSSGSAVSV 164

Query: 124 AAKLVDFSLGTDTGGSVRVPASYCGVLGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAI 183
            A+LVDFSLG DT G VRVPA++CG+LGFRPS G VS+ GV+P +QS +TVGWFA D ++
Sbjct: 165 GAELVDFSLGIDTTGGVRVPAAFCGILGFRPSQGTVSSVGVLPNSQSLETVGWFASDPSV 224

Query: 184 LKRVGWLLLQEPEVEHYKPTKVFIAEDCFKLLSSITSERLTQAFVSSVEKLFGGHLIKQI 243
           L +VG  LL    V H +   +  A+D F+ LS I  ++  Q    ++E L G    K +
Sbjct: 225 LCQVGHALLNLSAVTHRRQRSLIFADDLFE-LSDIPKQKSVQVVRKAIENLSGYKTPKHV 284

Query: 244 SLGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRLLQRHEFKINHGEWVGSYSSH 303
           ++G+YV   VPSL  F  E++   ++S  +L AL      +QRHEFK NH EW  +  S 
Sbjct: 285 NVGQYVASNVPSLAEF-CEQSGKSQNSASTLRALSSVMLAIQRHEFKTNHEEWWQTCKSF 344

Query: 304 LGPGISEQILEFIREATDENIDLSRSIQIELREVLAALLEDFGVLAIPTVPGPPPKLNTD 363
           LGP  S  ++  ++ + +E+I     ++ E+R  + +LL++ G+L IPTV  PPP+LNT 
Sbjct: 345 LGPRFSNDVVTALK-SKNESIKSLYRVKNEMRATIQSLLKEDGILVIPTVADPPPRLNTK 404

Query: 364 ISEQF-DFRAKAFSLLTIAAVSGVCQVSIPLGLYNGLPVSISLLAKHGSDGFLLNLVDCL 423
            ++   +F  + ++L  IA++SG CQV+IPLG +   P+S+SLL  +G D FLL+    +
Sbjct: 405 RNKSLNEFLDRTYALSCIASMSGCCQVTIPLGEHGDRPISVSLLTYYGGDKFLLDTTLDV 464

Query: 424 YSTLKEEVE 428
           Y++L+++ +
Sbjct: 465 YASLQDQAK 470

BLAST of MELO3C015329 vs. TAIR10
Match: AT3G25660.1 (AT3G25660.1 Amidase family protein)

HSP 1 Score: 104.4 bits (259), Expect = 1.8e-22
Identity = 61/149 (40.94%), Postives = 78/149 (52.35%), Query Frame = 1

Query: 25  PLSGLTFAVKDIFDIEGHVAGFGNPEWLRTHPPANQTAPAVLTILRGGATCIGKTMMDEM 84
           PL+G+   VKD    +G  +   +       PP + TA  V  I   G   +GKT MDE 
Sbjct: 107 PLAGVLIGVKDNICTQGMPSTAASRILEHYRPPFDATA--VKKIKELGGIVVGKTNMDEF 166

Query: 85  AFSINGENFHYGTPQNPCASGRIPGGSSSGSGVAVAAKLVDFSLGTDTGGSVRVPASYCG 144
                 E   +    NP    R+PGGSS GS  AVAA+    SLG+DTGGSVR PAS+CG
Sbjct: 167 GMGSTTEASAFQVTANPWDLSRVPGGSSGGSAAAVAARQCMVSLGSDTGGSVRQPASFCG 226

Query: 145 VLGFRPSHGVVSTSGVIPMTQSFDTVGWF 174
           V+G +P++G VS  G++    S D +G F
Sbjct: 227 VVGLKPTYGRVSRFGLMAYASSLDVIGCF 253

BLAST of MELO3C015329 vs. TAIR10
Match: AT5G64440.1 (AT5G64440.1 fatty acid amide hydrolase)

HSP 1 Score: 96.7 bits (239), Expect = 3.8e-20
Identity = 99/407 (24.32%), Postives = 160/407 (39.31%), Query Frame = 1

Query: 26  LSGLTFAVKDIFDIEGHVAGFGNPEWLRTHPPANQTAPAVLTILRGGATCIGKTMMDEMA 85
           L G+   +KD  D   H    G   WL       + +  V  +   GA  +GK  M E+ 
Sbjct: 197 LDGIFVTIKDDIDCLPHPTN-GGTTWLHEDRSVEKDSAVVSKLRSCGAILLGKANMHELG 256

Query: 86  FSINGENFHYGTPQNPCASGRIPGGSSSGSGVAVAAKLVDFSLGTDTGGSVRVPASYCGV 145
               G N +YGT +NP    R  GGSSSGS   VAA L   +LGTD GGSVR+P++ CG+
Sbjct: 257 MGTTGNNSNYGTTRNPHDPKRYTGGSSSGSAAIVAAGLCSAALGTDGGGSVRIPSALCGI 316

Query: 146 LGFRPSHGVVSTSGVIPMTQSFDTVGWFAR--DSAILKRVGWLLLQEPEVEHYKPTKVFI 205
            G + ++G    +G +    + + +G  A   + A L     L     +  + KP     
Sbjct: 317 TGLKTTYGRTDMTGSLCEGGTVEIIGPLASSLEDAFLVYAAILGSSSADRYNLKP----- 376

Query: 206 AEDCF-KLLSSITSERLTQAFVSSVEKLFGGHLIKQISLGKYVEDKVPSLKHFMIEENDG 265
           +  CF KLLS   S  +    +    K F       IS      DK   +   ++  N G
Sbjct: 377 SPPCFPKLLSHNGSNAIGSLRLGKYTKWFNDVSSSDIS------DKCEDILK-LLSNNHG 436

Query: 266 YK---HSIPSLAALVRSKRLLQRHEFKINHGEWVGSYSSHLGPGISEQI-------LEFI 325
            K     +P L      + +   H   I     + S + +   G + ++           
Sbjct: 437 CKVVEIVVPEL------EEMRAAHVISIG-SPTLSSLTPYCEAGKNSKLSYDTRTSFAIF 496

Query: 326 REATDENIDLSRSIQIELREVLAALLEDFGVLAIP----TVPGPPPKLNTDISEQFDFRA 385
           R  +  +   ++ ++  L E    + +D  V+  P    T P  PP    +         
Sbjct: 497 RSFSASDYIAAQCLRRRLMEYHLNIFKDVDVIVTPTTGMTAPVIPPDALKNGETNIQVTT 556

Query: 386 KAFSLLTIAAVSGVCQVSIPLGL-YNGLPVSISLLAKHGSDGFLLNL 415
                +  A + G   +S+P+G    GLP+ + ++ +  ++  +L L
Sbjct: 557 DLMRFVLAANLLGFPAISVPVGYDKEGLPIGLQIMGRPWAEATVLGL 583

BLAST of MELO3C015329 vs. NCBI nr
Match: gi|659099553|ref|XP_008450659.1| (PREDICTED: amidase 1-like [Cucumis melo])

HSP 1 Score: 859.0 bits (2218), Expect = 3.6e-246
Identity = 430/430 (100.00%), Postives = 430/430 (100.00%), Query Frame = 1

Query: 1   MAVDDCGAFIEKFLLQMSSPSDQLPLSGLTFAVKDIFDIEGHVAGFGNPEWLRTHPPANQ 60
           MAVDDCGAFIEKFLLQMSSPSDQLPLSGLTFAVKDIFDIEGHVAGFGNPEWLRTHPPANQ
Sbjct: 1   MAVDDCGAFIEKFLLQMSSPSDQLPLSGLTFAVKDIFDIEGHVAGFGNPEWLRTHPPANQ 60

Query: 61  TAPAVLTILRGGATCIGKTMMDEMAFSINGENFHYGTPQNPCASGRIPGGSSSGSGVAVA 120
           TAPAVLTILRGGATCIGKTMMDEMAFSINGENFHYGTPQNPCASGRIPGGSSSGSGVAVA
Sbjct: 61  TAPAVLTILRGGATCIGKTMMDEMAFSINGENFHYGTPQNPCASGRIPGGSSSGSGVAVA 120

Query: 121 AKLVDFSLGTDTGGSVRVPASYCGVLGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAIL 180
           AKLVDFSLGTDTGGSVRVPASYCGVLGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAIL
Sbjct: 121 AKLVDFSLGTDTGGSVRVPASYCGVLGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAIL 180

Query: 181 KRVGWLLLQEPEVEHYKPTKVFIAEDCFKLLSSITSERLTQAFVSSVEKLFGGHLIKQIS 240
           KRVGWLLLQEPEVEHYKPTKVFIAEDCFKLLSSITSERLTQAFVSSVEKLFGGHLIKQIS
Sbjct: 181 KRVGWLLLQEPEVEHYKPTKVFIAEDCFKLLSSITSERLTQAFVSSVEKLFGGHLIKQIS 240

Query: 241 LGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRLLQRHEFKINHGEWVGSYSSHL 300
           LGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRLLQRHEFKINHGEWVGSYSSHL
Sbjct: 241 LGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRLLQRHEFKINHGEWVGSYSSHL 300

Query: 301 GPGISEQILEFIREATDENIDLSRSIQIELREVLAALLEDFGVLAIPTVPGPPPKLNTDI 360
           GPGISEQILEFIREATDENIDLSRSIQIELREVLAALLEDFGVLAIPTVPGPPPKLNTDI
Sbjct: 301 GPGISEQILEFIREATDENIDLSRSIQIELREVLAALLEDFGVLAIPTVPGPPPKLNTDI 360

Query: 361 SEQFDFRAKAFSLLTIAAVSGVCQVSIPLGLYNGLPVSISLLAKHGSDGFLLNLVDCLYS 420
           SEQFDFRAKAFSLLTIAAVSGVCQVSIPLGLYNGLPVSISLLAKHGSDGFLLNLVDCLYS
Sbjct: 361 SEQFDFRAKAFSLLTIAAVSGVCQVSIPLGLYNGLPVSISLLAKHGSDGFLLNLVDCLYS 420

Query: 421 TLKEEVEAIY 431
           TLKEEVEAIY
Sbjct: 421 TLKEEVEAIY 430

BLAST of MELO3C015329 vs. NCBI nr
Match: gi|449435685|ref|XP_004135625.1| (PREDICTED: amidase 1-like [Cucumis sativus])

HSP 1 Score: 820.5 bits (2118), Expect = 1.4e-234
Identity = 410/430 (95.35%), Postives = 420/430 (97.67%), Query Frame = 1

Query: 1   MAVDDCGAFIEKFLLQMSSPSDQLPLSGLTFAVKDIFDIEGHVAGFGNPEWLRTHPPANQ 60
           MAVDD GAFIEKFLLQMSSPSD+LPLSGLTFAVKDIFDIEG+VAGFGNPEWLRTHPPANQ
Sbjct: 1   MAVDDYGAFIEKFLLQMSSPSDKLPLSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPANQ 60

Query: 61  TAPAVLTILRGGATCIGKTMMDEMAFSINGENFHYGTPQNPCASGRIPGGSSSGSGVAVA 120
           TAPAVLTILRGGATCIGKT+MDEMAFSINGENFHYGTPQNP AS RIPGGSSSGSGVAVA
Sbjct: 61  TAPAVLTILRGGATCIGKTIMDEMAFSINGENFHYGTPQNPHASDRIPGGSSSGSGVAVA 120

Query: 121 AKLVDFSLGTDTGGSVRVPASYCGVLGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAIL 180
           AKLVDFSLGTDTGGSVRVPASYCG+LGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAIL
Sbjct: 121 AKLVDFSLGTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAIL 180

Query: 181 KRVGWLLLQEPEVEHYKPTKVFIAEDCFKLLSSITSERLTQAFVSSVEKLFGGHLIKQIS 240
           KRVGWLLLQEPE+EHYKPTKVFIAEDCFKLLS+ITSERLTQAFVSSVEKLFGGHLIKQIS
Sbjct: 181 KRVGWLLLQEPEIEHYKPTKVFIAEDCFKLLSNITSERLTQAFVSSVEKLFGGHLIKQIS 240

Query: 241 LGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRLLQRHEFKINHGEWVGSYSSHL 300
           LGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKR LQRHEF INHGEWV SY SHL
Sbjct: 241 LGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRFLQRHEFNINHGEWVRSYHSHL 300

Query: 301 GPGISEQILEFIREATDENIDLSRSIQIELREVLAALLEDFGVLAIPTVPGPPPKLNTDI 360
           GPGISE+ILEFIRE TDENIDLSRSIQIELRE LAALLEDFGVLAIPTVPGPPPKLNTDI
Sbjct: 301 GPGISERILEFIREVTDENIDLSRSIQIELREALAALLEDFGVLAIPTVPGPPPKLNTDI 360

Query: 361 SEQFDFRAKAFSLLTIAAVSGVCQVSIPLGLYNGLPVSISLLAKHGSDGFLLNLVDCLYS 420
           SEQ+DFRAKAFSLLTIAAVSGVCQVS+PLGLYNGLPVSISLLAKHG+DGFLLNLVD LYS
Sbjct: 361 SEQYDFRAKAFSLLTIAAVSGVCQVSVPLGLYNGLPVSISLLAKHGADGFLLNLVDSLYS 420

Query: 421 TLKEEVEAIY 431
           TLKEEVEAIY
Sbjct: 421 TLKEEVEAIY 430

BLAST of MELO3C015329 vs. NCBI nr
Match: gi|449435683|ref|XP_004135624.1| (PREDICTED: amidase 1 [Cucumis sativus])

HSP 1 Score: 697.6 bits (1799), Expect = 1.4e-197
Identity = 347/430 (80.70%), Postives = 384/430 (89.30%), Query Frame = 1

Query: 1   MAVDDCGAFIEKFLLQMSSPSDQLPLSGLTFAVKDIFDIEGHVAGFGNPEWLRTHPPANQ 60
           MAV   GAF+EKFLLQ SSPSDQLPL+GLTFAVKDIFD++GHV GFGNPEWLRTHPPAN 
Sbjct: 1   MAVQCYGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANH 60

Query: 61  TAPAVLTILRGGATCIGKTMMDEMAFSINGENFHYGTPQNPCASGRIPGGSSSGSGVAVA 120
           TAP V TILRGGATCIG+T+MDEMA+SINGENFHYGTPQNPCAS R+PGGSSSGS VAV 
Sbjct: 61  TAPTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVG 120

Query: 121 AKLVDFSLGTDTGGSVRVPASYCGVLGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAIL 180
           A LVDFSLGTDTGGSVRVPASYCG+LGFRPSHG VSTSGV+PM QS DTVGWFARD  +L
Sbjct: 121 ANLVDFSLGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSMDTVGWFARDPVVL 180

Query: 181 KRVGWLLLQEPEVEHYKPTKVFIAEDCFKLLSSITSERLTQAFVSSVEKLFGGHLIKQIS 240
           K+VGWLLLQ+PEVEH KP +V IAEDCFK LSSI SERLTQAFV+SV+KLFGGH IK++S
Sbjct: 181 KKVGWLLLQQPEVEHQKPRQVLIAEDCFK-LSSIPSERLTQAFVNSVKKLFGGHPIKEVS 240

Query: 241 LGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRLLQRHEFKINHGEWVGSYSSHL 300
           LG YVE+KVPSLKHFMIE N G +HSIPSLAAL RS +LLQR+EFKINH EWV +Y+ HL
Sbjct: 241 LGNYVEEKVPSLKHFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHL 300

Query: 301 GPGISEQILEFIREATDENIDLSRSIQIELREVLAALLEDFGVLAIPTVPGPPPKLNTDI 360
           GPGISE++ E +R ATDENIDL  SI+IELR+ LAALLEDFGVLAIPTVPGPPPKLNTD+
Sbjct: 301 GPGISERVSEAMR-ATDENIDLCHSIRIELRKALAALLEDFGVLAIPTVPGPPPKLNTDV 360

Query: 361 SEQFDFRAKAFSLLTIAAVSGVCQVSIPLGLYNGLPVSISLLAKHGSDGFLLNLVDCLYS 420
           SE  DFRAKAFSLL+IA VSG CQVSIPLGLYNGLPVSISL+A HGSDGFLLN+V  LY+
Sbjct: 361 SELQDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLNVVHSLYN 420

Query: 421 TLKEEVEAIY 431
           TL+EEV+A +
Sbjct: 421 TLEEEVKASF 428

BLAST of MELO3C015329 vs. NCBI nr
Match: gi|659099551|ref|XP_008450658.1| (PREDICTED: amidase 1-like [Cucumis melo])

HSP 1 Score: 692.6 bits (1786), Expect = 4.4e-196
Identity = 344/424 (81.13%), Postives = 380/424 (89.62%), Query Frame = 1

Query: 7   GAFIEKFLLQMSSPSDQLPLSGLTFAVKDIFDIEGHVAGFGNPEWLRTHPPANQTAPAVL 66
           GAF+EKFLLQ SSPSDQLPL+GLTFAVKDIFD++GHV GFGNPEWLRTHPPAN TAP V 
Sbjct: 6   GAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANHTAPTVS 65

Query: 67  TILRGGATCIGKTMMDEMAFSINGENFHYGTPQNPCASGRIPGGSSSGSGVAVAAKLVDF 126
           TILRGGATCIG+T+MDEMA+SINGENFHYGTPQNPCAS R+PGGSSSGS VAV A LVDF
Sbjct: 66  TILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVGANLVDF 125

Query: 127 SLGTDTGGSVRVPASYCGVLGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAILKRVGWL 186
           SLGTDTGGSVRVPASYCG+LGFRPSHG VSTSGV+PM QSFDTVGWFARD  + K+VG L
Sbjct: 126 SLGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARDPVVFKKVGLL 185

Query: 187 LLQEPEVEHYKPTKVFIAEDCFKLLSSITSERLTQAFVSSVEKLFGGHLIKQISLGKYVE 246
           LLQ+PEVEH KPT+V IAEDCFK LSSI SERLTQAFV+SV+KLFGGHLIKQ++LG YVE
Sbjct: 186 LLQQPEVEHQKPTQVLIAEDCFK-LSSIPSERLTQAFVNSVKKLFGGHLIKQVNLGNYVE 245

Query: 247 DKVPSLKHFMIEENDGYKHSIPSLAALVRSKRLLQRHEFKINHGEWVGSYSSHLGPGISE 306
           +KVPSLK FMIE N G +HSIPSLAAL RS +LLQR+EFKINH EWV +Y+ HLGPGISE
Sbjct: 246 EKVPSLKQFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHLGPGISE 305

Query: 307 QILEFIREATDENIDLSRSIQIELREVLAALLEDFGVLAIPTVPGPPPKLNTDISEQFDF 366
           ++ E +R ATDENIDL  SI+IELR  LAALLEDFGVLAIPTVPGPPPKLNTD+SE  DF
Sbjct: 306 RVSEAMR-ATDENIDLCHSIKIELRRALAALLEDFGVLAIPTVPGPPPKLNTDVSELHDF 365

Query: 367 RAKAFSLLTIAAVSGVCQVSIPLGLYNGLPVSISLLAKHGSDGFLLNLVDCLYSTLKEEV 426
           RAKAFSLL+IA VSG CQVSIPLGLYNGLPVSISL+A HGSDGFLLN+V  LY+TL+EEV
Sbjct: 366 RAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLNVVHSLYNTLEEEV 425

Query: 427 EAIY 431
           +A +
Sbjct: 426 KASF 427

BLAST of MELO3C015329 vs. NCBI nr
Match: gi|223548325|gb|EEF49816.1| (amidase, putative [Ricinus communis])

HSP 1 Score: 575.5 bits (1482), Expect = 7.9e-161
Identity = 287/422 (68.01%), Postives = 343/422 (81.28%), Query Frame = 1

Query: 5   DCGAFIEKFLLQMSSPSDQLPLSGLTFAVKDIFDIEGHVAGFGNPEWLRTHPPANQTAPA 64
           D GAF+EKF+L+ SS SDQLPL  LTFAVKDIFD++G+V GFGNP+W RTH  A  TAPA
Sbjct: 78  DYGAFMEKFVLKPSSSSDQLPLHSLTFAVKDIFDVDGYVTGFGNPDWARTHSAATATAPA 137

Query: 65  VLTILRGGATCIGKTMMDEMAFSINGENFHYGTPQNPCASGRIPGGSSSGSGVAVAAKLV 124
           VL +LRGGATC+GK +MDEMA+SINGEN HYGTP NP A  R+PGGSSSGS VAV AKLV
Sbjct: 138 VLAVLRGGATCVGKVIMDEMAYSINGENAHYGTPTNPSAPDRVPGGSSSGSAVAVGAKLV 197

Query: 125 DFSLGTDTGGSVRVPASYCGVLGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAILKRVG 184
           DFSLG+DTGGSVRVPASYCG+LGFRPSHGVVS SGVIPM QSFDTVGWF+R+  ILK VG
Sbjct: 198 DFSLGSDTGGSVRVPASYCGILGFRPSHGVVSVSGVIPMAQSFDTVGWFSRNPVILKHVG 257

Query: 185 WLLLQEPEVEHYKPTKVFIAEDCFKLLSSITSERLTQAFVSSVEKLFGGHLIKQISLGKY 244
            LLLQ P+V+  KP ++FIAEDCF+ LSSI  +R++Q  V SVEKLFGG ++K ++LG Y
Sbjct: 258 RLLLQLPDVDRTKPNQIFIAEDCFQ-LSSIPKKRVSQVLVKSVEKLFGGDIVKHVNLGDY 317

Query: 245 VEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRLLQRHEFKINHGEWVGSYSSHLGPGI 304
           VEDKVPSL+HFM +E    +++I  LAAL  + R+LQR+EFK NHGEWV +     GPG+
Sbjct: 318 VEDKVPSLRHFMSKEIKEQEYNIAPLAALSSAMRMLQRYEFKTNHGEWVTTVKPDFGPGL 377

Query: 305 SEQILEFIREATDENIDLSRSIQIELREVLAALLEDFGVLAIPTVPGPPPKLNTDISEQF 364
            E+I E +R +T+ENID  +S++ ELR  L  LLE+FG++AIPTVPG PPKLNTD +   
Sbjct: 378 KERIWEAVR-STEENIDAFQSVRTELRAALTNLLEEFGIIAIPTVPGVPPKLNTDPTTLE 437

Query: 365 DFRAKAFSLLTIAAVSGVCQVSIPLGLYNGLPVSISLLAKHGSDGFLLNLVDCLYSTLKE 424
            FRAKAFSLL+I  VSG CQVSIPLG+++GLPVSISLLAKHGSD FLLN+V+ LY TL+E
Sbjct: 438 IFRAKAFSLLSITGVSGFCQVSIPLGMHDGLPVSISLLAKHGSDVFLLNVVETLYDTLQE 497

Query: 425 EV 427
           EV
Sbjct: 498 EV 497

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AMI1_ARATH8.2e-14262.05Amidase 1 OS=Arabidopsis thaliana GN=AMI1 PE=1 SV=1[more]
TOC64_PEA7.2e-11449.07Translocon at the outer membrane of chloroplasts 64 OS=Pisum sativum GN=TOC64 PE... [more]
OE64C_ARATH1.3e-11048.24Outer envelope protein 64, chloroplastic OS=Arabidopsis thaliana GN=OEP64 PE=1 S... [more]
OE64M_ARATH5.6e-9843.82Outer envelope protein 64, mitochondrial OS=Arabidopsis thaliana GN=OM64 PE=1 SV... [more]
GATA_SULTO7.9e-3628.54Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Sulfolobus tokodaii (strain DSM... [more]
Match NameE-valueIdentityDescription
A0A0A0LZ24_CUCSA9.8e-23595.35Uncharacterized protein OS=Cucumis sativus GN=Csa_1G570120 PE=4 SV=1[more]
A0A0A0M1K1_CUCSA9.6e-19880.70Uncharacterized protein OS=Cucumis sativus GN=Csa_1G570110 PE=4 SV=1[more]
B9RF45_RICCO5.5e-16168.01Amidase, putative OS=Ricinus communis GN=RCOM_1431340 PE=4 SV=1[more]
V4T529_9ROSI2.6e-15867.69Uncharacterized protein OS=Citrus clementina GN=CICLE_v10001213mg PE=4 SV=1[more]
A5BL97_VITVI2.2e-15766.67Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_015334 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G08980.14.6e-14362.05 amidase 1[more]
AT3G17970.17.2e-11248.24 translocon at the outer membrane of chloroplasts 64-III[more]
AT5G09420.13.1e-9943.82 translocon at the outer membrane of chloroplasts 64-V[more]
AT3G25660.11.8e-2240.94 Amidase family protein[more]
AT5G64440.13.8e-2024.32 fatty acid amide hydrolase[more]
Match NameE-valueIdentityDescription
gi|659099553|ref|XP_008450659.1|3.6e-246100.00PREDICTED: amidase 1-like [Cucumis melo][more]
gi|449435685|ref|XP_004135625.1|1.4e-23495.35PREDICTED: amidase 1-like [Cucumis sativus][more]
gi|449435683|ref|XP_004135624.1|1.4e-19780.70PREDICTED: amidase 1 [Cucumis sativus][more]
gi|659099551|ref|XP_008450658.1|4.4e-19681.13PREDICTED: amidase 1-like [Cucumis melo][more]
gi|223548325|gb|EEF49816.1|7.9e-16168.01amidase, putative [Ricinus communis][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000120Amidase
IPR020556Amidase_CS
IPR023631Amidase_dom
Vocabulary: Molecular Function
TermDefinition
GO:0016884carbon-nitrogen ligase activity, with glutamine as amido-N-donor
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006525 arginine metabolic process
biological_process GO:0018874 benzoate metabolic process
biological_process GO:0030154 cell differentiation
biological_process GO:0019761 glucosinolate biosynthetic process
biological_process GO:0009684 indoleacetic acid biosynthetic process
biological_process GO:0009965 leaf morphogenesis
biological_process GO:0006558 L-phenylalanine metabolic process
biological_process GO:0045893 positive regulation of transcription, DNA-templated
biological_process GO:0006560 proline metabolic process
biological_process GO:0042207 styrene catabolic process
biological_process GO:0006568 tryptophan metabolic process
cellular_component GO:0005829 cytosol
molecular_function GO:0004040 amidase activity
molecular_function GO:0016884 carbon-nitrogen ligase activity, with glutamine as amido-N-donor
molecular_function GO:0043864 indoleacetamide hydrolase activity
This gene is associated with the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
MU61435melon EST collection version 4.0transcribed_cluster

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO3C015329T1MELO3C015329T1mRNA


The following transcribed_cluster feature(s) are associated with this gene:

Feature NameUnique NameType
MU61435MU61435transcribed_cluster


Analysis Name: InterPro Annotations of melon
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000120AmidasePANTHERPTHR11895AMIDASEcoord: 4..429
score: 4.1E
IPR020556Amidase, conserved sitePROSITEPS00571AMIDASEScoord: 109..140
scor
IPR023631Amidase signature domainGENE3DG3DSA:3.90.1300.10coord: 21..418
score: 1.0
IPR023631Amidase signature domainPFAMPF01425Amidasecoord: 310..412
score: 5.4E-8coord: 20..176
score: 1.2
IPR023631Amidase signature domainunknownSSF75304Amidase signature (AS) enzymescoord: 13..414
score: 2.49
NoneNo IPR availablePANTHERPTHR11895:SF39OUTER ENVELOPE PROTEIN 64, CHLOROPLASTICcoord: 4..429
score: 4.1E

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None