MELO3C013943 (gene) Melon (DHL92) v3.5.1

NameMELO3C013943
Typegene
OrganismCucumis melo (Melon (DHL92) v3.5.1)
DescriptionEukaryotic aspartyl protease family protein
Locationchr6 : 33665039 .. 33666899 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAACCCCATCTTCCCCTAACCAGAACCTTCTTCTTCTTCTTCTATTCTCAATCCCCATCTCTTTAAATTCTCCAATTTCTCTCTCTAATTTCATTTCAAAAACCCCCCAATTTCTCTCTCTAAAATACTCCATTAATGGCGGTTTCCCCTGTTTTCATCTTCCTCCTCTGTTTTCTCCTCTCCTCCCCTGTTTTCTCCTCACAAATTTTCCTTCTACCTCTCTCCCATTCCTTATCATCCTCAATCTCCGATTTCAACAGCACCCACAATCTTCTCAAATCCACCGCCACCCGCTCCTCCGCCCGATTCCACCGCCACCGCCATAACCACCTCTCTCTGCCCCTATCCCCCGGCGGCGATTACACTCTCTCCTTCAACCTCGGCTCTGAGTCTCACAAAATTTCCCTCTATATGGACACCGGCAGCGACCTCGTTTGGTTCCCCTGTTCCCCGTTTGAGTGTATTCTTTGTGAAGGTAAACCAAAAATTCAATCCCCTTTGCCCAAAATCTCAAATAACAAATCAGTTTCCTGCAGCGCCGCCGCCTGCTCCGCCGCTCATGGTGGCTCCCTCTCCGCTTCCCACCTCTGTGCAATTTCTCGATGTCCACTTGAATCCATTGAAATTTCTGAGTGCTCCTCTTTTTCCTGTCCTCCGTTTTATTACGCTTACGGCGATGGGAGTTTAGTTGCTCGGCTTTATAGAGATAGCCTCAGTTTGCCAACGCCAGCGCCATCTCCGCCGATTAATGTTCGGAATTTTACTTTTGGATGTGCCCACACGACGCTTGGCGAACCGGTTGGGGTTGCCGGATTCGGCCGTGGGGTGTTGTCGATGCCCAGTCAACTCGCGACTTTCTCACCTCAACTCGGGAACCGGTTTTCTTATTGTTTGGTTTCTCACTCGTTTGCGGCGGACCGAGTTCGCCGCCCGAGTCCACTGATTCTCGGGCGGTACCACACCGGGGAGACGGAGTTCATTTACACTTCCTTGCTTGAGAATCCAAAACACCCTTATTTTTACTCAGTTGGATTGGCCGGAATATCAGTTGGGAATGTGAGGATTCCAGCGCCGGAGTTCTTGAGAAAAGTTGATGAGAGTGGTAGTGGCGGCGTTGTGGTGGATTCCGGCACTACTTTTACTATGCTCCCGTCAGGTTTGTATGAATCGGTGGTGGCCGAGTTCGAAAACCGTACCGGAAAGGTTGCAAACCGGGCGAGACGGATCGAAGAAAACACCGGGTTGAGCCCTTGCTATTACTACGAGAACTCAGTTGGCGTGCCACGTGTCGTGCTACATTTTGTTGGGGAGAAATCCAGTGTGGTGCTTCCTAGGAAGAACTATTTCTATGAGTTTTTGGACGGTGGAGATGGGGTGGTGGAGGTGGGGAGGAAGAGAAAAGTTGGGTGTTTGATGCTAATGAACGGTGGAGATGAGGCTGAGCTGGCAGGTGGGCCTGGTGCCACGCTTGGGAACTACCAACAACAAGGTTTTGAGGTGGTTTATGATTTGGAAAAGAACCGGGTTGGATTCGCTCGGCGGCAATGCTCCACTCTTTGGGACAATTTGAACCGGAGTTAGTGATGAAGAGTGAACCCGGTTGAGAAAGTGTTCTTTGACTTGTGACTATTGTCAACGGTCAACGCTATGAGGGTAAATAAGGAAATTTCAGGTTTGAAGGTTTTATTTTTTGTTGTAAATTCCTTGGGCACTTCACTTCTTGCTTTAATTATTATTTTTAGTTGAAATTTGTATATTAAAAGTGTTCAAAAAATTTGTTGCAAAGAAGAAAAAAAAATGTATAGCTCAATTTTATTGCATGAAGCAAGGAATGAAGTGGTTGCTTTATCAAGTTAAGAA

mRNA sequence

AAAACCCCATCTTCCCCTAACCAGAACCTTCTTCTTCTTCTTCTATTCTCAATCCCCATCTCTTTAAATTCTCCAATTTCTCTCTCTAATTTCATTTCAAAAACCCCCCAATTTCTCTCTCTAAAATACTCCATTAATGGCGGTTTCCCCTGTTTTCATCTTCCTCCTCTGTTTTCTCCTCTCCTCCCCTGTTTTCTCCTCACAAATTTTCCTTCTACCTCTCTCCCATTCCTTATCATCCTCAATCTCCGATTTCAACAGCACCCACAATCTTCTCAAATCCACCGCCACCCGCTCCTCCGCCCGATTCCACCGCCACCGCCATAACCACCTCTCTCTGCCCCTATCCCCCGGCGGCGATTACACTCTCTCCTTCAACCTCGGCTCTGAGTCTCACAAAATTTCCCTCTATATGGACACCGGCAGCGACCTCGTTTGGTTCCCCTGTTCCCCGTTTGAGTGTATTCTTTGTGAAGGTAAACCAAAAATTCAATCCCCTTTGCCCAAAATCTCAAATAACAAATCAGTTTCCTGCAGCGCCGCCGCCTGCTCCGCCGCTCATGGTGGCTCCCTCTCCGCTTCCCACCTCTGTGCAATTTCTCGATGTCCACTTGAATCCATTGAAATTTCTGAGTGCTCCTCTTTTTCCTGTCCTCCGTTTTATTACGCTTACGGCGATGGGAGTTTAGTTGCTCGGCTTTATAGAGATAGCCTCAGTTTGCCAACGCCAGCGCCATCTCCGCCGATTAATGTTCGGAATTTTACTTTTGGATGTGCCCACACGACGCTTGGCGAACCGGTTGGGGTTGCCGGATTCGGCCGTGGGGTGTTGTCGATGCCCAGTCAACTCGCGACTTTCTCACCTCAACTCGGGAACCGGTTTTCTTATTGTTTGGTTTCTCACTCGTTTGCGGCGGACCGAGTTCGCCGCCCGAGTCCACTGATTCTCGGGCGGTACCACACCGGGGAGACGGAGTTCATTTACACTTCCTTGCTTGAGAATCCAAAACACCCTTATTTTTACTCAGTTGGATTGGCCGGAATATCAGTTGGGAATGTGAGGATTCCAGCGCCGGAGTTCTTGAGAAAAGTTGATGAGAGTGGTAGTGGCGGCGTTGTGGTGGATTCCGGCACTACTTTTACTATGCTCCCGTCAGGTTTGTATGAATCGGTGGTGGCCGAGTTCGAAAACCGTACCGGAAAGGTTGCAAACCGGGCGAGACGGATCGAAGAAAACACCGGGTTGAGCCCTTGCTATTACTACGAGAACTCAGTTGGCGTGCCACGTGTCGTGCTACATTTTGTTGGGGAGAAATCCAGTGTGGTGCTTCCTAGGAAGAACTATTTCTATGAGTTTTTGGACGGTGGAGATGGGGTGGTGGAGGTGGGGAGGAAGAGAAAAGTTGGGTGTTTGATGCTAATGAACGGTGGAGATGAGGCTGAGCTGGCAGGTGGGCCTGGTGCCACGCTTGGGAACTACCAACAACAAGGTTTTGAGGTGGTTTATGATTTGGAAAAGAACCGGGTTGGATTCGCTCGGCGGCAATGCTCCACTCTTTGGGACAATTTGAACCGGAGTTAGTGATGAAGAGTGAACCCGGTTGAGAAAGTGTTCTTTGACTTGTGACTATTGTCAACGGTCAACGCTATGAGGGTAAATAAGGAAATTTCAGGTTTGAAGGTTTTATTTTTTGTTGTAAATTCCTTGGGCACTTCACTTCTTGCTTTAATTATTATTTTTAGTTGAAATTTGTATATTAAAAGTGTTCAAAAAATTTGTTGCAAAGAAGAAAAAAAAATGTATAGCTCAATTTTATTGCATGAAGCAAGGAATGAAGTGGTTGCTTTATCAAGTTAAGAA

Coding sequence (CDS)

ATGGCGGTTTCCCCTGTTTTCATCTTCCTCCTCTGTTTTCTCCTCTCCTCCCCTGTTTTCTCCTCACAAATTTTCCTTCTACCTCTCTCCCATTCCTTATCATCCTCAATCTCCGATTTCAACAGCACCCACAATCTTCTCAAATCCACCGCCACCCGCTCCTCCGCCCGATTCCACCGCCACCGCCATAACCACCTCTCTCTGCCCCTATCCCCCGGCGGCGATTACACTCTCTCCTTCAACCTCGGCTCTGAGTCTCACAAAATTTCCCTCTATATGGACACCGGCAGCGACCTCGTTTGGTTCCCCTGTTCCCCGTTTGAGTGTATTCTTTGTGAAGGTAAACCAAAAATTCAATCCCCTTTGCCCAAAATCTCAAATAACAAATCAGTTTCCTGCAGCGCCGCCGCCTGCTCCGCCGCTCATGGTGGCTCCCTCTCCGCTTCCCACCTCTGTGCAATTTCTCGATGTCCACTTGAATCCATTGAAATTTCTGAGTGCTCCTCTTTTTCCTGTCCTCCGTTTTATTACGCTTACGGCGATGGGAGTTTAGTTGCTCGGCTTTATAGAGATAGCCTCAGTTTGCCAACGCCAGCGCCATCTCCGCCGATTAATGTTCGGAATTTTACTTTTGGATGTGCCCACACGACGCTTGGCGAACCGGTTGGGGTTGCCGGATTCGGCCGTGGGGTGTTGTCGATGCCCAGTCAACTCGCGACTTTCTCACCTCAACTCGGGAACCGGTTTTCTTATTGTTTGGTTTCTCACTCGTTTGCGGCGGACCGAGTTCGCCGCCCGAGTCCACTGATTCTCGGGCGGTACCACACCGGGGAGACGGAGTTCATTTACACTTCCTTGCTTGAGAATCCAAAACACCCTTATTTTTACTCAGTTGGATTGGCCGGAATATCAGTTGGGAATGTGAGGATTCCAGCGCCGGAGTTCTTGAGAAAAGTTGATGAGAGTGGTAGTGGCGGCGTTGTGGTGGATTCCGGCACTACTTTTACTATGCTCCCGTCAGGTTTGTATGAATCGGTGGTGGCCGAGTTCGAAAACCGTACCGGAAAGGTTGCAAACCGGGCGAGACGGATCGAAGAAAACACCGGGTTGAGCCCTTGCTATTACTACGAGAACTCAGTTGGCGTGCCACGTGTCGTGCTACATTTTGTTGGGGAGAAATCCAGTGTGGTGCTTCCTAGGAAGAACTATTTCTATGAGTTTTTGGACGGTGGAGATGGGGTGGTGGAGGTGGGGAGGAAGAGAAAAGTTGGGTGTTTGATGCTAATGAACGGTGGAGATGAGGCTGAGCTGGCAGGTGGGCCTGGTGCCACGCTTGGGAACTACCAACAACAAGGTTTTGAGGTGGTTTATGATTTGGAAAAGAACCGGGTTGGATTCGCTCGGCGGCAATGCTCCACTCTTTGGGACAATTTGAACCGGAGTTAG

Protein sequence

MAVSPVFIFLLCFLLSSPVFSSQIFLLPLSHSLSSSISDFNSTHNLLKSTATRSSARFHRHRHNHLSLPLSPGGDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQSPLPKISNNKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPPFYYAYGDGSLVARLYRDSLSLPTPAPSPPINVRNFTFGCAHTTLGEPVGVAGFGRGVLSMPSQLATFSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYHTGETEFIYTSLLENPKHPYFYSVGLAGISVGNVRIPAPEFLRKVDESGSGGVVVDSGTTFTMLPSGLYESVVAEFENRTGKVANRARRIEENTGLSPCYYYENSVGVPRVVLHFVGEKSSVVLPRKNYFYEFLDGGDGVVEVGRKRKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDNLNRS*
BLAST of MELO3C013943 vs. Swiss-Prot
Match: ASP63_ARATH (Probable aspartyl protease At4g16563 OS=Arabidopsis thaliana GN=At4g16563 PE=2 SV=1)

HSP 1 Score: 589.3 bits (1518), Expect = 3.7e-167
Identity = 301/480 (62.71%), Postives = 357/480 (74.38%), Query Frame = 1

Query: 26  LLPLSHSLSSSISDFNSTHNLLKSTATRSSARFHRHRHNH----LSLPLSPGGDYTLSFN 85
           LL LSHSLS+S    +  H LLKS+++RSSARF RH H      LSLP+S G DY +S +
Sbjct: 30  LLHLSHSLSTSKHSSSPLH-LLKSSSSRSSARFRRHHHKQQQQQLSLPISSGSDYLISLS 89

Query: 86  LGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQSPLPKISNNKS-VSCSAAACSA 145
           +GS S  +SLY+DTGSDLVWFPC PF CILCE KP   SP   +S++ + VSCS+ +CSA
Sbjct: 90  VGSSSSAVSLYLDTGSDLVWFPCRPFTCILCESKPLPPSPPSSLSSSATTVSCSSPSCSA 149

Query: 146 AHGGSLSASHLCAISRCPLESIEISEC--SSFSCPPFYYAYGDGSLVARLYRDSLSLPTP 205
           AH  SL +S LCAIS CPL+ IE  +C  SS+ CPPFYYAYGDGSLVA+LY DSLSLP+ 
Sbjct: 150 AHS-SLPSSDLCAISNCPLDFIETGDCNTSSYPCPPFYYAYGDGSLVAKLYSDSLSLPS- 209

Query: 206 APSPPINVRNFTFGCAHTTLGEPVGVAGFGRGVLSMPSQLATFSPQLGNRFSYCLVSHSF 265
                ++V NFTFGCAHTTL EP+GVAGFGRG LS+P+QLA  SP LGN FSYCLVSHSF
Sbjct: 210 -----VSVSNFTFGCAHTTLAEPIGVAGFGRGRLSLPAQLAVHSPHLGNSFSYCLVSHSF 269

Query: 266 AADRVRRPSPLILGRY------HTGET--------------EFIYTSLLENPKHPYFYSV 325
            +DRVRRPSPLILGR+        G T              EF++T +LENPKHPYFYSV
Sbjct: 270 DSDRVRRPSPLILGRFVDKKEKRVGTTDDHDDGDDEKKKKNEFVFTEMLENPKHPYFYSV 329

Query: 326 GLAGISVGNVRIPAPEFLRKVDESGSGGVVVDSGTTFTMLPSGLYESVVAEFENRTGKVA 385
            L GIS+G   IPAP  LR++D++G GGVVVDSGTTFTMLP+  Y SVV EF++R G+V 
Sbjct: 330 SLQGISIGKRNIPAPAMLRRIDKNGGGGVVVDSGTTFTMLPAKFYNSVVEEFDSRVGRVH 389

Query: 386 NRARRIEENTGLSPCYYYENSVGVPRVVLHFVGEKSSVVLPRKNYFYEFLDGGDGVVEVG 445
            RA R+E ++G+SPCYY   +V VP +VLHF G +SSV LPR+NYFYEF+DGGDG  E  
Sbjct: 390 ERADRVEPSSGMSPCYYLNQTVKVPALVLHFAGNRSSVTLPRRNYFYEFMDGGDGKEE-- 449

Query: 446 RKRKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDNL 479
            KRK+GCLMLMNGGDE+EL GG GA LGNYQQQGFEVVYDL   RVGFA+R+C++LWD+L
Sbjct: 450 -KRKIGCLMLMNGGDESELRGGTGAILGNYQQQGFEVVYDLLNRRVGFAKRKCASLWDSL 498

BLAST of MELO3C013943 vs. Swiss-Prot
Match: APF2_ARATH (Aspartyl protease family protein 2 OS=Arabidopsis thaliana GN=APF2 PE=2 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 2.2e-34
Identity = 133/413 (32.20%), Postives = 181/413 (43.83%), Query Frame = 1

Query: 70  LSPG-GDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQSPLPKISNN 129
           LS G G+Y     +G+ +  + + +DTGSD+VW  C+P  C  C  +       P     
Sbjct: 135 LSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAP--CRRCYSQSD-----PIFDPR 194

Query: 130 KSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPPFYYAYGDGSL-VAR 189
           KS + +   CS+ H   L ++      +  L  +               +YGDGS  V  
Sbjct: 195 KSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQV---------------SYGDGSFTVGD 254

Query: 190 LYRDSLSLPTPAPSPPINVRNFTFGCAHTTLGEPVGVAGF---GRGVLSMPSQLATFSPQ 249
              ++L+           V+    GC H   G  VG AG    G+G LS P Q      +
Sbjct: 255 FSTETLTFRRN------RVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGH---R 314

Query: 250 LGNRFSYCLVSHSFAADRVRRPSPLILGRYHTGETEFIYTSLLENPKHPYFYSVGLAGIS 309
              +FSYCLV  S ++    +PS ++ G          +T LL NPK   FY VGL GIS
Sbjct: 315 FNQKFSYCLVDRSASS----KPSSVVFGNAAVSRIAR-FTPLLSNPKLDTFYYVGLLGIS 374

Query: 310 VGNVRIPA-PEFLRKVDESGSGGVVVDSGTTFTMLPSGLYESVVAEFENRTGKVANRARR 369
           VG  R+P     L K+D+ G+GGV++DSGT+ T L    Y ++   F  R G  A   +R
Sbjct: 375 VGGTRVPGVTASLFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAF--RVG--AKTLKR 434

Query: 370 IEENTGLSPCYYYEN--SVGVPRVVLHFVGEKSSVVLPRKNYFYEFLDGGDGVVEVGRKR 429
             + +    C+   N   V VP VVLHF G  + V LP  NY                  
Sbjct: 435 APDFSLFDTCFDLSNMNEVKVPTVVLHFRG--ADVSLPATNYLIP--------------- 485

Query: 430 KVGCLMLMNGGDEAELAGGPG--ATLGNYQQQGFEVVYDLEKNRVGFARRQCS 473
                +  NG      AG  G  + +GN QQQGF VVYDL  +RVGFA   C+
Sbjct: 495 -----VDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDLASSRVGFAPGGCA 485

BLAST of MELO3C013943 vs. Swiss-Prot
Match: ASPG1_ARATH (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 3.1e-33
Identity = 127/423 (30.02%), Postives = 174/423 (41.13%), Query Frame = 1

Query: 60  RHRHNHLSLPLSPG-----GDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEG 119
           R++   L+ P+  G     G+Y     +G+ + ++ L +DTGSD+ W  C P  C  C  
Sbjct: 141 RYQTEDLTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVLDTGSDVNWIQCEP--CADCYQ 200

Query: 120 KPKIQSPLPKISNNKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPP 179
           +          S  KS++CSA  CS                      +E S C S  C  
Sbjct: 201 QSDPVFNPTSSSTYKSLTCSAPQCSL---------------------LETSACRSNKCL- 260

Query: 180 FYYAYGDGSL-VARLYRDSLSLPTPAPSPPINVRNFTFGCAHTTLGEPVGVAGF---GRG 239
           +  +YGDGS  V  L  D+++           + N   GC H   G   G AG    G G
Sbjct: 261 YQVSYGDGSFTVGELATDTVTFGNSG-----KINNVALGCGHDNEGLFTGAAGLLGLGGG 320

Query: 240 VLSMPSQLATFSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYHTGETEFIYTSLLENP 299
           VLS+ +Q+   S      FSYCLV          + S L       G  +     LL N 
Sbjct: 321 VLSITNQMKATS------FSYCLVDRDSG-----KSSSLDFNSVQLGGGDAT-APLLRNK 380

Query: 300 KHPYFYSVGLAGISVGNVRIPAPEFLRKVDESGSGGVVVDSGTTFTMLPSGLYESVVAEF 359
           K   FY VGL+G SVG  ++  P+ +  VD SGSGGV++D GT  T L +  Y S+   F
Sbjct: 381 KIDTFYYVGLSGFSVGGEKVVLPDAIFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAF 440

Query: 360 ENRTGKVANRARRIEENTGLSPCYYYE--NSVGVPRVVLHFVGEKSSVVLPRKNYFYEFL 419
              T    N  +     +    CY +   ++V VP V  HF G K S+ LP KNY     
Sbjct: 441 LKLT---VNLKKGSSSISLFDTCYDFSSLSTVKVPTVAFHFTGGK-SLDLPAKNYLIPVD 500

Query: 420 DGGDGVVEVGRKRKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFAR 472
           D G             C           +       +GN QQQG  + YDL KN +G + 
Sbjct: 501 DSG-----------TFCFAFAPTSSSLSI-------IGNVQQQGTRITYDLSKNVIGLSG 500

BLAST of MELO3C013943 vs. Swiss-Prot
Match: NEP1_NEPGR (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis GN=nep1 PE=1 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 7.0e-33
Identity = 120/409 (29.34%), Postives = 183/409 (44.74%), Query Frame = 1

Query: 74  GDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQSPLPKISNNKSVSC 133
           G+Y ++ ++G+ +   S  MDTGSDL+W  C P  C  C          P  +   S S 
Sbjct: 93  GEYLMNLSIGTPAQPFSAIMDTGSDLIWTQCQP--CTQC-----FNQSTPIFNPQGSSSF 152

Query: 134 SAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPPFYYAYGDGSLV-ARLYRDS 193
           S   CS         S LC       +++    CS+  C  + Y YGDGS     +  ++
Sbjct: 153 STLPCS---------SQLC-------QALSSPTCSNNFCQ-YTYGYGDGSETQGSMGTET 212

Query: 194 LSLPTPAPSPPINVRNFTFGCAHTTLG----EPVGVAGFGRGVLSMPSQLATFSPQLGNR 253
           L+  +      +++ N TFGC     G       G+ G GRG LS+PSQL         +
Sbjct: 213 LTFGS------VSIPNITFGCGENNQGFGQGNGAGLVGMGRGPLSLPSQLDV------TK 272

Query: 254 FSYCLVSHSFAADRVRRPSPLILGRYHTGETEFI-YTSLLENPKHPYFYSVGLAGISVGN 313
           FSYC+     +      PS L+LG      T     T+L+++ + P FY + L G+SVG+
Sbjct: 273 FSYCMTPIGSST-----PSNLLLGSLANSVTAGSPNTTLIQSSQIPTFYYITLNGLSVGS 332

Query: 314 VRIPA-PEFLRKVDESGSGGVVVDSGTTFTMLPSGLYESVVAEFENRTG-KVANRARRIE 373
            R+P  P        +G+GG+++DSGTT T   +  Y+SV  EF ++    V N +    
Sbjct: 333 TRLPIDPSAFALNSNNGTGGIIIDSGTTLTYFVNNAYQSVRQEFISQINLPVVNGS---- 392

Query: 374 ENTGLSPCYYY---ENSVGVPRVVLHFVGEKSSVVLPRKNYFYEFLDGGDGVVEVGRKRK 433
            ++G   C+      +++ +P  V+HF G    + LP +NY   F+   +G++       
Sbjct: 393 -SSGFDLCFQTPSDPSNLQIPTFVMHFDG--GDLELPSENY---FISPSNGLI------- 434

Query: 434 VGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQC 472
             CL + +      +        GN QQQ   VVYD   + V FA  QC
Sbjct: 453 --CLAMGSSSQGMSI-------FGNIQQQNMLVVYDTGNSVVSFASAQC 434

BLAST of MELO3C013943 vs. Swiss-Prot
Match: NEP2_NEPGR (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis GN=nep2 PE=1 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 4.5e-32
Identity = 117/407 (28.75%), Postives = 176/407 (43.24%), Query Frame = 1

Query: 74  GDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQSPLPKISNNKSVSC 133
           G+Y ++  +G+     S  MDTGSDL+W  C P  C  C        P P  +   S S 
Sbjct: 94  GEYLMNVAIGTPDSSFSAIMDTGSDLIWTQCEP--CTQC-----FSQPTPIFNPQDSSSF 153

Query: 134 SAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPPFYYAYGDGSLV-ARLYRDS 193
           S   C + +   L           P E+   +EC       + Y YGDGS     +  ++
Sbjct: 154 STLPCESQYCQDL-----------PSETCNNNECQ------YTYGYGDGSTTQGYMATET 213

Query: 194 LSLPTPAPSPPINVRNFTFGCAHTTLG----EPVGVAGFGRGVLSMPSQLATFSPQLGNR 253
            +  T +      V N  FGC     G       G+ G G G LS+PSQL         +
Sbjct: 214 FTFETSS------VPNIAFGCGEDNQGFGQGNGAGLIGMGWGPLSLPSQLGV------GQ 273

Query: 254 FSYCLVSHSFAADRVRRPSPLILGRYHTGETEFI-YTSLLENPKHPYFYSVGLAGISVGN 313
           FSYC+ S+  ++     PS L LG   +G  E    T+L+ +  +P +Y + L GI+VG 
Sbjct: 274 FSYCMTSYGSSS-----PSTLALGSAASGVPEGSPSTTLIHSSLNPTYYYITLQGITVGG 333

Query: 314 VRIPAPEFLRKVDESGSGGVVVDSGTTFTMLPSGLYESVVAEFENRTGKVANRARRIEEN 373
             +  P    ++ + G+GG+++DSGTT T LP   Y +V   F ++     N     E +
Sbjct: 334 DNLGIPSSTFQLQDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFTDQ----INLPTVDESS 393

Query: 374 TGLSPCYYYE---NSVGVPRVVLHFVGEKSSVVLPRKNYFYEFLDGGDGVVEVGRKRKVG 433
           +GLS C+      ++V VP + + F G                L+ G+  + +     V 
Sbjct: 394 SGLSTCFQQPSDGSTVQVPEISMQFDG--------------GVLNLGEQNILISPAEGVI 435

Query: 434 CLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQC 472
           CL +   G  ++L     +  GN QQQ  +V+YDL+   V F   QC
Sbjct: 454 CLAM---GSSSQLG---ISIFGNIQQQETQVLYDLQNLAVSFVPTQC 435

BLAST of MELO3C013943 vs. TrEMBL
Match: A0A0A0L5I7_CUCSA (Pepsin A OS=Cucumis sativus GN=Csa_3G020060 PE=3 SV=1)

HSP 1 Score: 961.4 bits (2484), Expect = 4.0e-277
Identity = 471/481 (97.92%), Postives = 477/481 (99.17%), Query Frame = 1

Query: 1   MAVSPVFIFLLCFLLSSPVFSSQIFLLPLSHSLSSSISDFNSTHNLLKSTATRSSARFHR 60
           MAVSPVFIFLLCFLLSSPVFSSQIFLLPLSHSLSSSISDFN+THNLLKSTATRSSARFHR
Sbjct: 1   MAVSPVFIFLLCFLLSSPVFSSQIFLLPLSHSLSSSISDFNNTHNLLKSTATRSSARFHR 60

Query: 61  HRHNHLSLPLSPGGDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQS 120
           HRHNHLSLPLSPGGDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQS
Sbjct: 61  HRHNHLSLPLSPGGDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQS 120

Query: 121 PLPKISNNKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPPFYYAYG 180
           PLPKI+NNKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPPFYYAYG
Sbjct: 121 PLPKIANNKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPPFYYAYG 180

Query: 181 DGSLVARLYRDSLSLPTPAPSPPINVRNFTFGCAHTTLGEPVGVAGFGRGVLSMPSQLAT 240
           DGSLVARLYRDSLSLPTPAPSPPINVRNFTFGCAHTTLGEPVGVAGFGRGVLSMPSQLAT
Sbjct: 181 DGSLVARLYRDSLSLPTPAPSPPINVRNFTFGCAHTTLGEPVGVAGFGRGVLSMPSQLAT 240

Query: 241 FSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYHTGETEFIYTSLLENPKHPYFYSVGL 300
           FSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRY+TGETEFIYTSLLENPKHPYFYSVGL
Sbjct: 241 FSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYYTGETEFIYTSLLENPKHPYFYSVGL 300

Query: 301 AGISVGNVRIPAPEFLRKVDESGSGGVVVDSGTTFTMLPSGLYESVVAEFENRTGKVANR 360
           AGISVGN+RIPAPEFL KVDE GSGGVVVDSGTTFTMLP+GLYESVVAEFENRTGKVANR
Sbjct: 301 AGISVGNIRIPAPEFLTKVDEGGSGGVVVDSGTTFTMLPAGLYESVVAEFENRTGKVANR 360

Query: 361 ARRIEENTGLSPCYYYENSVGVPRVVLHFVGEKSSVVLPRKNYFYEFLDGGDGVVEVGRK 420
           ARRIEENTGLSPCYYYENSVGVPRVVLHFVGEKS+VVLPRKNYFYEFLDGGDGV  VGRK
Sbjct: 361 ARRIEENTGLSPCYYYENSVGVPRVVLHFVGEKSNVVLPRKNYFYEFLDGGDGV--VGRK 420

Query: 421 RKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDNLNR 480
           RKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDNLNR
Sbjct: 421 RKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDNLNR 479

Query: 481 S 482
           S
Sbjct: 481 S 479

BLAST of MELO3C013943 vs. TrEMBL
Match: B9GYA7_POPTR (Aspartyl protease family protein OS=Populus trichocarpa GN=POPTR_0003s07390g PE=3 SV=1)

HSP 1 Score: 660.6 bits (1703), Expect = 1.5e-186
Identity = 341/498 (68.47%), Postives = 389/498 (78.11%), Query Frame = 1

Query: 10  LLCFLLSSP---VFSSQIFLLPLSHSLSSSISDFNSTHNLLKSTATRSSARFHRHRHN-- 69
           LLCF+L      + +SQ   LPL HSLS +   F STH+LLKST+TRS+ RFH H HN  
Sbjct: 8   LLCFILCFTHIFISTSQTLFLPLIHSLSKT--QFTSTHHLLKSTSTRSTTRFHHHHHNKN 67

Query: 70  -----HLSLPLSPGGDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQ 129
                 +SLPLSPG DYTLSF + S+   ISLY+DTGSDLVWFPC PFECILCEGK +  
Sbjct: 68  SHNHRQVSLPLSPGSDYTLSFTINSQP--ISLYLDTGSDLVWFPCQPFECILCEGKAENA 127

Query: 130 S----PLPKISNNKS-VSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPP 189
           S    P PK+S   + VSC ++ACSA H  +L +S LCAIS CPLESIEIS+C   SCP 
Sbjct: 128 SLASTPPPKLSKTATPVSCKSSACSAVHS-NLPSSDLCAISNCPLESIEISDCRKHSCPQ 187

Query: 190 FYYAYGDGSLVARLYRDSLSLPTPAPSPPINVRNFTFGCAHTTLGEPVGVAGFGRGVLSM 249
           FYYAYGDGSL+ARLYRDS+ LP    +  I   NFTFGCAHTTL EP+GVAGFGRGVLS+
Sbjct: 188 FYYAYGDGSLIARLYRDSIRLPLSNQTNLI-FNNFTFGCAHTTLAEPIGVAGFGRGVLSL 247

Query: 250 PSQLATFSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYHTGETE----------FIYT 309
           P+QLAT SPQLGN+FSYCLVSHSF +DRVRRPSPLILGRY   E E          F+YT
Sbjct: 248 PAQLATLSPQLGNQFSYCLVSHSFDSDRVRRPSPLILGRYDHDEKERRVNGVKKPSFVYT 307

Query: 310 SLLENPKHPYFYSVGLAGISVGNVRIPAPEFLRKVDESGSGGVVVDSGTTFTMLPSGLYE 369
           S+L+NP+HPYFY VGL GIS+G  +IPAP+FLRKVD  GSGGVVVDSGTTFTMLP+ LY+
Sbjct: 308 SMLDNPRHPYFYCVGLEGISIGRKKIPAPDFLRKVDRKGSGGVVVDSGTTFTMLPASLYD 367

Query: 370 SVVAEFENRTGKVANRARRIEENTGLSPCYYYENS-VGVPRVVLHFVGEKSSVVLPRKNY 429
            VVAEFENR G+V  RA  IEENTGLSPCYY++N+ V VPRVVLHFVG  SSVVLPR+NY
Sbjct: 368 FVVAEFENRVGRVNERASVIEENTGLSPCYYFDNNVVNVPRVVLHFVGNGSSVVLPRRNY 427

Query: 430 FYEFLDGGDGVVEVGRKRKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNR 482
           FYEFLDGG G    G+KRKVGCLMLMNGGDEAEL+GGPGATLGNYQQQGFEVVYDLE  R
Sbjct: 428 FYEFLDGGHG---KGKKRKVGCLMLMNGGDEAELSGGPGATLGNYQQQGFEVVYDLENRR 487

BLAST of MELO3C013943 vs. TrEMBL
Match: B9NGC6_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0001s15870g PE=3 SV=1)

HSP 1 Score: 652.9 bits (1683), Expect = 3.0e-184
Identity = 342/500 (68.40%), Postives = 388/500 (77.60%), Query Frame = 1

Query: 7   FIFLLCFLLSSPVF---SSQIFLLPLSHSLSSSISDFNSTHNLLKSTATRSSARFHRHRH 66
           +  LLCF L    F   +SQ   LPL+HSLS +   F STH+L+KST+T S  RF RH H
Sbjct: 5   YSLLLCFSLCFSHFFISTSQTLFLPLTHSLSKT--QFTSTHHLIKSTSTSSITRFRRHHH 64

Query: 67  -----NH--LSLPLSPGGDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEGKP 126
                NH  +SLPLSPG DYTLSF L  +S  I LY+DTGSDLVWFPC PFECILCEGK 
Sbjct: 65  QKNTHNHRQVSLPLSPGSDYTLSFTL--DSQPIFLYLDTGSDLVWFPCQPFECILCEGKA 124

Query: 127 KIQS----PLPKISNNKS-VSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFS 186
           +  S    P PK+S   + VSC ++ACSAAH  +L +S LCAIS CPLESIE S+C   S
Sbjct: 125 ENTSLASTPPPKLSKTATPVSCKSSACSAAHS-NLPSSDLCAISNCPLESIETSDCQKHS 184

Query: 187 CPPFYYAYGDGSLVARLYRDSLSLPTPAPSPPINVRNFTFGCAHTTLGEPVGVAGFGRGV 246
           CP FYYAYGDGSL+ARLYRDS+SLP   P+  I V NFTFGCAHT L EP+GVAGFGRGV
Sbjct: 185 CPQFYYAYGDGSLIARLYRDSISLPLSNPTNLI-VNNFTFGCAHTALAEPIGVAGFGRGV 244

Query: 247 LSMPSQLATFSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYHTGETE----------F 306
           LS+P+QLAT SPQLGN+FSYCLVSHSF +DR+RRPSPLILGRY   E E          F
Sbjct: 245 LSLPAQLATLSPQLGNQFSYCLVSHSFDSDRLRRPSPLILGRYDHDEKERRVNGVNKPRF 304

Query: 307 IYTSLLENPKHPYFYSVGLAGISVGNVRIPAPEFLRKVDESGSGGVVVDSGTTFTMLPSG 366
           +YTS+L+N +HPYFY VGL GIS+G  +IPAP FLRKVD  GSGG+VVDSGTTFTMLP+ 
Sbjct: 305 VYTSMLDNLEHPYFYCVGLEGISIGRKKIPAPGFLRKVDGEGSGGLVVDSGTTFTMLPAS 364

Query: 367 LYESVVAEFENRTGKVANRARRIEENTGLSPCYYYENS-VGVPRVVLHFVGEKSSVVLPR 426
           LY SVVAEFENR G+V  RAR IEE+TGLSPCYY++N+ V VP VVLHFVG  SSVVLPR
Sbjct: 365 LYGSVVAEFENRVGRVNERARVIEEDTGLSPCYYFDNNVVNVPSVVLHFVGNGSSVVLPR 424

Query: 427 KNYFYEFLDGGDGVVEVGRKRKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLE 481
           +NYFYEFLDGGDG    G+KRKVGCLMLMNGGDEAEL+GGPGATLGNYQQQGFEVVYDLE
Sbjct: 425 RNYFYEFLDGGDG---KGKKRKVGCLMLMNGGDEAELSGGPGATLGNYQQQGFEVVYDLE 484

BLAST of MELO3C013943 vs. TrEMBL
Match: B9SSF8_RICCO (Pepsin A, putative OS=Ricinus communis GN=RCOM_1061010 PE=3 SV=1)

HSP 1 Score: 650.6 bits (1677), Expect = 1.5e-183
Identity = 326/491 (66.40%), Postives = 393/491 (80.04%), Query Frame = 1

Query: 9   FLLCFLLSSPVFSSQIFLLPLSHSLSSSISDFNSTHNLLKSTATRSSARF-HRHRHNHL- 68
           F+LCF   S V  S+I  LPL+HSLS++   F STH+LLKST++RS++RF H+H+  HL 
Sbjct: 11  FILCFSCIS-VSISEILYLPLTHSLSNT--QFTSTHHLLKSTSSRSASRFQHQHQKRHLR 70

Query: 69  -----SLPLSPGGDYTLSFNLGSESHK-ISLYMDTGSDLVWFPCSPFECILCEGKPK--- 128
                SLPLSPG DYTLSF L S   + +SLY+DTGSDLVWFPC PFECILCEGK +   
Sbjct: 71  NRHQVSLPLSPGSDYTLSFTLNSNPPQHVSLYLDTGSDLVWFPCKPFECILCEGKAENTT 130

Query: 129 IQSPLPKISNN-KSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPPFY 188
             +P P++S+  +SV C ++ACSAAH  +L  S LCAI+ CPLESIE S+C SFSCP FY
Sbjct: 131 ASTPPPRLSSTARSVHCKSSACSAAHS-NLPTSDLCAIADCPLESIETSDCHSFSCPSFY 190

Query: 189 YAYGDGSLVARLYRDSLSLPTPAPSPPINVRNFTFGCAHTTLGEPVGVAGFGRGVLSMPS 248
           YAYGDGSLVARLY DS+ LP   PS  +++ NFTFGCAHT L EPVGVAGFGRGVLS+P+
Sbjct: 191 YAYGDGSLVARLYHDSIKLPLATPS--LSLHNFTFGCAHTALAEPVGVAGFGRGVLSLPA 250

Query: 249 QLATFSPQLGNRFSYCLVSHSFAADRVRRPSPLILG-------RYHTGETEFIYTSLLEN 308
           QLA+F+PQLGNRFSYCLVSHSF +DR+R PSPLILG       R +  + +F+YTS+L+N
Sbjct: 251 QLASFAPQLGNRFSYCLVSHSFNSDRLRLPSPLILGHSDDKEKRVNKDDVQFVYTSMLDN 310

Query: 309 PKHPYFYSVGLAGISVGNVRIPAPEFLRKVDESGSGGVVVDSGTTFTMLPSGLYESVVAE 368
           PKHPYFY VGL GIS+G  +IPAPEFL++VD  GSGGVVVDSGTTFTMLP+ LY SVVAE
Sbjct: 311 PKHPYFYCVGLEGISIGKKKIPAPEFLKRVDREGSGGVVVDSGTTFTMLPASLYNSVVAE 370

Query: 369 FENRTGKVANRARRIEENTGLSPCYYYENSVGVPRVVLHFVGEKSSVVLPRKNYFYEFLD 428
           F+NR G+V  RA+ +E+ TGL PCYYY+  V +P +VLHFVG +SSVVLP+KNYFY+FLD
Sbjct: 371 FDNRVGRVYERAKEVEDKTGLGPCYYYDTVVNIPSLVLHFVGNESSVVLPKKNYFYDFLD 430

Query: 429 GGDGVVEVGRKRKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARR 481
           GGDG   V RKR+VGCLMLMNGG+EAEL GGPGATLGNYQQ GFEVVYDLE+ RVGFARR
Sbjct: 431 GGDG---VRRKRRVGCLMLMNGGEEAELTGGPGATLGNYQQHGFEVVYDLEQRRVGFARR 490

BLAST of MELO3C013943 vs. TrEMBL
Match: A0A061G3B8_THECC (Eukaryotic aspartyl protease family protein OS=Theobroma cacao GN=TCM_015978 PE=3 SV=1)

HSP 1 Score: 643.7 bits (1659), Expect = 1.8e-181
Identity = 324/489 (66.26%), Postives = 382/489 (78.12%), Query Frame = 1

Query: 1   MAVSPVFIFLLCFLLSSPVFS-SQIFLLPLSHSLSSSISDFNSTHNLLKSTATRSSARFH 60
           MA S   ++LL F       S S++ LLPL+HSLS +   F + H+LLK+T+ RS+ARFH
Sbjct: 1   MATSLCLLYLLLFSTCWLTISMSEMVLLPLTHSLSKT--QFTTPHHLLKTTSARSAARFH 60

Query: 61  R--HRHNHLSLPLSPGGDYTLSFNLGSESHK-ISLYMDTGSDLVWFPCSPFECILCEGKP 120
           +  HRH  +SLPLSPG DYTLSF LGS S   ISLY+DTGSDLVW PCSPFECILCE KP
Sbjct: 61  QRNHRHKQVSLPLSPGSDYTLSFTLGSPSSPTISLYLDTGSDLVWLPCSPFECILCESKP 120

Query: 121 KIQSPLPKISNNKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPPFY 180
            +  PL   S+  +V C ++ACSAAH  SL +S LCAI+RCPL+SIE S+C+SF CPPFY
Sbjct: 121 PLSPPLNLSSSATAVPCKSSACSAAHS-SLPSSDLCAIARCPLDSIETSDCNSFPCPPFY 180

Query: 181 YAYGDGSLVARLYRDSLSLPTPAPSPPINVRNFTFGCAHTTLGEPVGVAGFGRGVLSMPS 240
           YAYGDGSL+ARL++DSL+LP       ++++NFTFGCAHT LGEPVGVAGFG G LS+P+
Sbjct: 181 YAYGDGSLIARLFKDSLTLPNS-----LSIQNFTFGCAHTALGEPVGVAGFGFGRLSLPA 240

Query: 241 QLATFSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYHTGE-------TEFIYTSLLEN 300
           QL++ SPQLGNRFSYCLVSHSF +D+V RPSPLILGR+   E        EF+YT +L N
Sbjct: 241 QLSSVSPQLGNRFSYCLVSHSFDSDKVGRPSPLILGRHEEQEKQFGNENVEFVYTDMLSN 300

Query: 301 PKHPYFYSVGLAGISVGNVRIPAPEFLRKVDESGSGGVVVDSGTTFTMLPSGLYESVVAE 360
           PKHPYFYSVG  GISVG   IPAPE L++VD  GSGG+VVDSGTTFTMLP+ LY+SVV E
Sbjct: 301 PKHPYFYSVGFEGISVGKRNIPAPENLKRVDRRGSGGMVVDSGTTFTMLPASLYDSVVTE 360

Query: 361 FENRTGKVANRARRIEENTGLSPCYYYENSVGVPRVVLHFVGEKSSVVLPRKNYFYEFLD 420
           F++R G+V  RA  +EE TG+SPCYYY+    VP + LHFVG  SSVVLPR+NYFYEFLD
Sbjct: 361 FDHRVGRVYERASEVEETTGMSPCYYYDKVAKVPVISLHFVGNGSSVVLPRRNYFYEFLD 420

Query: 421 GGDGVVEVGRKRKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARR 479
           GGDG   V +KR VGCLMLMNGGDE EL GGPGATLGNYQQQGFEVVYDLEK +VGFARR
Sbjct: 421 GGDG---VRKKRNVGCLMLMNGGDEKELIGGPGATLGNYQQQGFEVVYDLEKRKVGFARR 478

BLAST of MELO3C013943 vs. TAIR10
Match: AT4G16563.1 (AT4G16563.1 Eukaryotic aspartyl protease family protein)

HSP 1 Score: 589.3 bits (1518), Expect = 2.1e-168
Identity = 301/480 (62.71%), Postives = 357/480 (74.38%), Query Frame = 1

Query: 26  LLPLSHSLSSSISDFNSTHNLLKSTATRSSARFHRHRHNH----LSLPLSPGGDYTLSFN 85
           LL LSHSLS+S    +  H LLKS+++RSSARF RH H      LSLP+S G DY +S +
Sbjct: 30  LLHLSHSLSTSKHSSSPLH-LLKSSSSRSSARFRRHHHKQQQQQLSLPISSGSDYLISLS 89

Query: 86  LGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQSPLPKISNNKS-VSCSAAACSA 145
           +GS S  +SLY+DTGSDLVWFPC PF CILCE KP   SP   +S++ + VSCS+ +CSA
Sbjct: 90  VGSSSSAVSLYLDTGSDLVWFPCRPFTCILCESKPLPPSPPSSLSSSATTVSCSSPSCSA 149

Query: 146 AHGGSLSASHLCAISRCPLESIEISEC--SSFSCPPFYYAYGDGSLVARLYRDSLSLPTP 205
           AH  SL +S LCAIS CPL+ IE  +C  SS+ CPPFYYAYGDGSLVA+LY DSLSLP+ 
Sbjct: 150 AHS-SLPSSDLCAISNCPLDFIETGDCNTSSYPCPPFYYAYGDGSLVAKLYSDSLSLPS- 209

Query: 206 APSPPINVRNFTFGCAHTTLGEPVGVAGFGRGVLSMPSQLATFSPQLGNRFSYCLVSHSF 265
                ++V NFTFGCAHTTL EP+GVAGFGRG LS+P+QLA  SP LGN FSYCLVSHSF
Sbjct: 210 -----VSVSNFTFGCAHTTLAEPIGVAGFGRGRLSLPAQLAVHSPHLGNSFSYCLVSHSF 269

Query: 266 AADRVRRPSPLILGRY------HTGET--------------EFIYTSLLENPKHPYFYSV 325
            +DRVRRPSPLILGR+        G T              EF++T +LENPKHPYFYSV
Sbjct: 270 DSDRVRRPSPLILGRFVDKKEKRVGTTDDHDDGDDEKKKKNEFVFTEMLENPKHPYFYSV 329

Query: 326 GLAGISVGNVRIPAPEFLRKVDESGSGGVVVDSGTTFTMLPSGLYESVVAEFENRTGKVA 385
            L GIS+G   IPAP  LR++D++G GGVVVDSGTTFTMLP+  Y SVV EF++R G+V 
Sbjct: 330 SLQGISIGKRNIPAPAMLRRIDKNGGGGVVVDSGTTFTMLPAKFYNSVVEEFDSRVGRVH 389

Query: 386 NRARRIEENTGLSPCYYYENSVGVPRVVLHFVGEKSSVVLPRKNYFYEFLDGGDGVVEVG 445
            RA R+E ++G+SPCYY   +V VP +VLHF G +SSV LPR+NYFYEF+DGGDG  E  
Sbjct: 390 ERADRVEPSSGMSPCYYLNQTVKVPALVLHFAGNRSSVTLPRRNYFYEFMDGGDGKEE-- 449

Query: 446 RKRKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDNL 479
            KRK+GCLMLMNGGDE+EL GG GA LGNYQQQGFEVVYDL   RVGFA+R+C++LWD+L
Sbjct: 450 -KRKIGCLMLMNGGDESELRGGTGAILGNYQQQGFEVVYDLLNRRVGFAKRKCASLWDSL 498

BLAST of MELO3C013943 vs. TAIR10
Match: AT5G45120.1 (AT5G45120.1 Eukaryotic aspartyl protease family protein)

HSP 1 Score: 210.3 bits (534), Expect = 2.6e-54
Identity = 163/502 (32.47%), Postives = 230/502 (45.82%), Query Frame = 1

Query: 6   VFIFLLCFLLSSPVFSSQIFLLPLSHSLSSSISDFNSTHNLLKSTATRSSARFHRHRHNH 65
           +F+FLL  LL +    +Q       H   SS S       L KS+ +  + +        
Sbjct: 8   LFLFLLITLLLNTTNKTQA----RQHKNPSSSSSSFLVLTLTKSSVSLPTPKSQTQERIK 67

Query: 66  LSL--------PLSPGGD-YTLSFNLGSESHKISLYMDTGSDLVWFPCS--PFECILCEG 125
             L        PL    D Y ++ N+G+    + +Y+DTGSDL W PC    F+CI C  
Sbjct: 68  KPLSSVDVVMEPLREVRDGYLITLNIGTPPQAVQVYLDTGSDLTWVPCGNLSFDCIECYD 127

Query: 126 -------KPKIQSPLPKISNNKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEISEC 185
                   P + SPL   ++ +  SC+++ C   H    +    CA++ C +  +  S C
Sbjct: 128 LKNNDLKSPSVFSPLHSSTSFRD-SCASSFCVEIHSSD-NPFDPCAVAGCSVSMLLKSTC 187

Query: 186 SSFSCPPFYYAYGDGSLVAR-LYRDSLSLPTPAPSPPINVRNFTFGCAHTTLGEPVGVAG 245
               CP F Y YG+G L++  L RD L   T       +V  F+FGC  +T  EP+G+AG
Sbjct: 188 VR-PCPSFAYTYGEGGLISGILTRDILKARTR------DVPRFSFGCVTSTYREPIGIAG 247

Query: 246 FGRGVLSMPSQLATFSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYHTG---ETEFIY 305
           FGRG+LS+PSQL      L   FS+C +   F  +     SPLILG             +
Sbjct: 248 FGRGLLSLPSQLGF----LEKGFSHCFLPFKF-VNNPNISSPLILGASALSINLTDSLQF 307

Query: 306 TSLLENPKHPYFYSVGLAGISVGNVRIP--APEFLRKVDESGSGGVVVDSGTTFTMLPSG 365
           T +L  P +P  Y +GL  I++G    P   P  LR+ D  G+GG++VDSGTT+T LP  
Sbjct: 308 TPMLNTPMYPNSYYIGLESITIGTNITPTQVPLTLRQFDSQGNGGMLVDSGTTYTHLPEP 367

Query: 366 LYESVVAEFENRTGKVANRARRIEENTGLSPCY----------YYENSVGV--PRVVLHF 425
            Y  ++   ++       RA   E  TG   CY            EN V +  P +  HF
Sbjct: 368 FYSQLLTTLQSTI--TYPRATETESRTGFDLCYKVPCPNNNLTSLENDVMMIFPSITFHF 427

Query: 426 VGEKSSVVLPRKNYFYEFLDGGDGVVEVGRKRKVGCLMLMNGGDEAELAGGPGATLGNYQ 472
           +   ++++LP+ N FY      DG V       V CL+  N  D      GP    G++Q
Sbjct: 428 L-NNATLLLPQGNSFYAMSAPSDGSV-------VQCLLFQNMEDGDY---GPAGVFGSFQ 478

BLAST of MELO3C013943 vs. TAIR10
Match: AT3G52500.1 (AT3G52500.1 Eukaryotic aspartyl protease family protein)

HSP 1 Score: 188.0 bits (476), Expect = 1.4e-47
Identity = 154/507 (30.37%), Postives = 226/507 (44.58%), Query Frame = 1

Query: 4   SPVFIFLLCFLLSSPVFSSQIFLLPLSHSLSSSISDFNSTHNLLKSTATRSSARFHRHRH 63
           S +F F L FL  S V + ++ L P SHS  S    + S   L +S    S AR H+ +H
Sbjct: 3   SSIFFFFLIFL--SVVSAVKLPLSPFSHSDQSPKDPYLSLRRLAES----SIARAHKLKH 62

Query: 64  NH-------------------LSLPLSPG--GDYTLSFNLGSESHKISLYMDTGSDLVWF 123
                                +  PLS    G Y++S + G+ S  I    DTGS LVW 
Sbjct: 63  GTSIKPDEDALSSTTTASATVVKSPLSAKSYGGYSVSLSFGTPSQTIPFVFDTGSSLVWL 122

Query: 124 PC-SPFECILCEGKPKIQSPLPKI-----SNNKSVSCSAAACSAAHGGSLSASHLCAISR 183
           PC S + C  C+      + +P+      S++K + C +  C   +G ++        +R
Sbjct: 123 PCTSRYLCSGCDFSGLDPTLIPRFIPKNSSSSKIIGCQSPKCQFLYGPNVQCRGCDPNTR 182

Query: 184 CPLESIEISECSSFSCPPFYYAYGDGSLVARLYRDSLSLPTPAPSPPINVRNFTFGCAHT 243
                     C+   CPP+   YG GS    L  + L  P       + V +F  GC+  
Sbjct: 183 ---------NCT-VGCPPYILQYGLGSTAGVLITEKLDFPD------LTVPDFVVGCSII 242

Query: 244 TLGEPVGVAGFGRGVLSMPSQLATFSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYHT 303
           +  +P G+AGFGRG +S+PSQ+         RFS+CLVS  F    V     L  G  H 
Sbjct: 243 STRQPAGIAGFGRGPVSLPSQMNL------KRFSHCLVSRRFDDTNVTTDLDLDTGSGHN 302

Query: 304 GETE---FIYTSLLENPKHPY-----FYSVGLAGISVGNVRIPAPEFLRKVDESGSGGVV 363
             ++     YT   +NP         +Y + L  I VG   +  P        +G GG +
Sbjct: 303 SGSKTPGLTYTPFRKNPNVSNKAFLEYYYLNLRRIYVGRKHVKIPYKYLAPGTNGDGGSI 362

Query: 364 VDSGTTFTMLPSGLYESVVAEFENRTGKVANRARRIEENTGLSPCYYY--ENSVGVPRVV 423
           VDSG+TFT +   ++E V  EF ++      R + +E+ TGL PC+    +  V VP ++
Sbjct: 363 VDSGSTFTFMERPVFELVAEEFASQMSNYT-REKDLEKETGLGPCFNISGKGDVTVPELI 422

Query: 424 LHFVGEKSSVVLPRKNYFYEFLDGGDGVVEVGRKRKVGCLMLMNGGDEAELAG-GPGATL 473
             F G  + + LP  NYF  F+   D V          CL +++        G GP   L
Sbjct: 423 FEFKGG-AKLELPLSNYF-TFVGNTDTV----------CLTVVSDKTVNPSGGTGPAIIL 468

BLAST of MELO3C013943 vs. TAIR10
Match: AT3G61820.1 (AT3G61820.1 Eukaryotic aspartyl protease family protein)

HSP 1 Score: 148.7 bits (374), Expect = 9.4e-36
Identity = 130/409 (31.78%), Postives = 175/409 (42.79%), Query Frame = 1

Query: 70  LSPG-GDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQSPLPKISNN 129
           LS G G+Y +   +G+ +  + + +DTGSD+VW  CSP  C  C  +        K    
Sbjct: 128 LSQGSGEYFMRLGVGTPATNVYMVLDTGSDVVWLQCSP--CKACYNQTDAIFDPKKSKTF 187

Query: 130 KSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPPFYYAYGDGSLVARL 189
            +V C +  C       L  S  C   R      ++S             YGDGS     
Sbjct: 188 ATVPCGSRLCRR-----LDDSSECVTRRSKTCLYQVS-------------YGDGSFTEGD 247

Query: 190 YRDSLSLPTPAPSPPINVRNFTFGCAHTTLGEPVGVAGF---GRGVLSMPSQLATFSPQL 249
           +         A      V +   GC H   G  VG AG    GRG LS PSQ      + 
Sbjct: 248 FSTETLTFHGA-----RVDHVPLGCGHDNEGLFVGAAGLLGLGRGGLSFPSQTKN---RY 307

Query: 250 GNRFSYCLVSHSFAADRVRRPSPLILGRYHTGETEFIYTSLLENPKHPYFYSVGLAGISV 309
             +FSYCLV  + +    + PS ++ G     +T  ++T LL NPK   FY + L GISV
Sbjct: 308 NGKFSYCLVDRTSSGSSSKPPSTIVFGNAAVPKTS-VFTPLLTNPKLDTFYYLQLLGISV 367

Query: 310 GNVRIPA-PEFLRKVDESGSGGVVVDSGTTFTMLPSGLYESVVAEFENRTGKVANRARRI 369
           G  R+P   E   K+D +G+GGV++DSGT+ T L    Y ++   F  R G  A + +R 
Sbjct: 368 GGSRVPGVSESQFKLDATGNGGVIIDSGTSVTRLTQPAYVALRDAF--RLG--ATKLKRA 427

Query: 370 EENTGLSPCYYYEN--SVGVPRVVLHFVGEKSSVVLPRKNYFYEFLDGGDGVVEVGRKRK 429
              +    C+      +V VP VV HF G    V LP  NY          ++ V  +  
Sbjct: 428 PSYSLFDTCFDLSGMTTVKVPTVVFHFGG--GEVSLPASNY----------LIPVNTE-- 483

Query: 430 VGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQC 472
            G       G    L+      +GN QQQGF V YDL  +RVGF  R C
Sbjct: 488 -GRFCFAFAGTMGSLS-----IIGNIQQQGFRVAYDLVGSRVGFLSRAC 483

BLAST of MELO3C013943 vs. TAIR10
Match: AT1G01300.1 (AT1G01300.1 Eukaryotic aspartyl protease family protein)

HSP 1 Score: 148.3 bits (373), Expect = 1.2e-35
Identity = 133/413 (32.20%), Postives = 181/413 (43.83%), Query Frame = 1

Query: 70  LSPG-GDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQSPLPKISNN 129
           LS G G+Y     +G+ +  + + +DTGSD+VW  C+P  C  C  +       P     
Sbjct: 135 LSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAP--CRRCYSQSD-----PIFDPR 194

Query: 130 KSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPPFYYAYGDGSL-VAR 189
           KS + +   CS+ H   L ++      +  L  +               +YGDGS  V  
Sbjct: 195 KSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQV---------------SYGDGSFTVGD 254

Query: 190 LYRDSLSLPTPAPSPPINVRNFTFGCAHTTLGEPVGVAGF---GRGVLSMPSQLATFSPQ 249
              ++L+           V+    GC H   G  VG AG    G+G LS P Q      +
Sbjct: 255 FSTETLTFRRN------RVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGH---R 314

Query: 250 LGNRFSYCLVSHSFAADRVRRPSPLILGRYHTGETEFIYTSLLENPKHPYFYSVGLAGIS 309
              +FSYCLV  S ++    +PS ++ G          +T LL NPK   FY VGL GIS
Sbjct: 315 FNQKFSYCLVDRSASS----KPSSVVFGNAAVSRIAR-FTPLLSNPKLDTFYYVGLLGIS 374

Query: 310 VGNVRIPA-PEFLRKVDESGSGGVVVDSGTTFTMLPSGLYESVVAEFENRTGKVANRARR 369
           VG  R+P     L K+D+ G+GGV++DSGT+ T L    Y ++   F  R G  A   +R
Sbjct: 375 VGGTRVPGVTASLFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAF--RVG--AKTLKR 434

Query: 370 IEENTGLSPCYYYEN--SVGVPRVVLHFVGEKSSVVLPRKNYFYEFLDGGDGVVEVGRKR 429
             + +    C+   N   V VP VVLHF G  + V LP  NY                  
Sbjct: 435 APDFSLFDTCFDLSNMNEVKVPTVVLHFRG--ADVSLPATNYLIP--------------- 485

Query: 430 KVGCLMLMNGGDEAELAGGPG--ATLGNYQQQGFEVVYDLEKNRVGFARRQCS 473
                +  NG      AG  G  + +GN QQQGF VVYDL  +RVGFA   C+
Sbjct: 495 -----VDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDLASSRVGFAPGGCA 485

BLAST of MELO3C013943 vs. NCBI nr
Match: gi|659095959|ref|XP_008448851.1| (PREDICTED: aspartic proteinase nepenthesin-1 [Cucumis melo])

HSP 1 Score: 981.1 bits (2535), Expect = 7.0e-283
Identity = 481/481 (100.00%), Postives = 481/481 (100.00%), Query Frame = 1

Query: 1   MAVSPVFIFLLCFLLSSPVFSSQIFLLPLSHSLSSSISDFNSTHNLLKSTATRSSARFHR 60
           MAVSPVFIFLLCFLLSSPVFSSQIFLLPLSHSLSSSISDFNSTHNLLKSTATRSSARFHR
Sbjct: 1   MAVSPVFIFLLCFLLSSPVFSSQIFLLPLSHSLSSSISDFNSTHNLLKSTATRSSARFHR 60

Query: 61  HRHNHLSLPLSPGGDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQS 120
           HRHNHLSLPLSPGGDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQS
Sbjct: 61  HRHNHLSLPLSPGGDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQS 120

Query: 121 PLPKISNNKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPPFYYAYG 180
           PLPKISNNKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPPFYYAYG
Sbjct: 121 PLPKISNNKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPPFYYAYG 180

Query: 181 DGSLVARLYRDSLSLPTPAPSPPINVRNFTFGCAHTTLGEPVGVAGFGRGVLSMPSQLAT 240
           DGSLVARLYRDSLSLPTPAPSPPINVRNFTFGCAHTTLGEPVGVAGFGRGVLSMPSQLAT
Sbjct: 181 DGSLVARLYRDSLSLPTPAPSPPINVRNFTFGCAHTTLGEPVGVAGFGRGVLSMPSQLAT 240

Query: 241 FSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYHTGETEFIYTSLLENPKHPYFYSVGL 300
           FSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYHTGETEFIYTSLLENPKHPYFYSVGL
Sbjct: 241 FSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYHTGETEFIYTSLLENPKHPYFYSVGL 300

Query: 301 AGISVGNVRIPAPEFLRKVDESGSGGVVVDSGTTFTMLPSGLYESVVAEFENRTGKVANR 360
           AGISVGNVRIPAPEFLRKVDESGSGGVVVDSGTTFTMLPSGLYESVVAEFENRTGKVANR
Sbjct: 301 AGISVGNVRIPAPEFLRKVDESGSGGVVVDSGTTFTMLPSGLYESVVAEFENRTGKVANR 360

Query: 361 ARRIEENTGLSPCYYYENSVGVPRVVLHFVGEKSSVVLPRKNYFYEFLDGGDGVVEVGRK 420
           ARRIEENTGLSPCYYYENSVGVPRVVLHFVGEKSSVVLPRKNYFYEFLDGGDGVVEVGRK
Sbjct: 361 ARRIEENTGLSPCYYYENSVGVPRVVLHFVGEKSSVVLPRKNYFYEFLDGGDGVVEVGRK 420

Query: 421 RKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDNLNR 480
           RKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDNLNR
Sbjct: 421 RKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDNLNR 480

Query: 481 S 482
           S
Sbjct: 481 S 481

BLAST of MELO3C013943 vs. NCBI nr
Match: gi|449458942|ref|XP_004147205.1| (PREDICTED: aspartic proteinase nepenthesin-1 [Cucumis sativus])

HSP 1 Score: 961.4 bits (2484), Expect = 5.7e-277
Identity = 471/481 (97.92%), Postives = 477/481 (99.17%), Query Frame = 1

Query: 1   MAVSPVFIFLLCFLLSSPVFSSQIFLLPLSHSLSSSISDFNSTHNLLKSTATRSSARFHR 60
           MAVSPVFIFLLCFLLSSPVFSSQIFLLPLSHSLSSSISDFN+THNLLKSTATRSSARFHR
Sbjct: 1   MAVSPVFIFLLCFLLSSPVFSSQIFLLPLSHSLSSSISDFNNTHNLLKSTATRSSARFHR 60

Query: 61  HRHNHLSLPLSPGGDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQS 120
           HRHNHLSLPLSPGGDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQS
Sbjct: 61  HRHNHLSLPLSPGGDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQS 120

Query: 121 PLPKISNNKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPPFYYAYG 180
           PLPKI+NNKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPPFYYAYG
Sbjct: 121 PLPKIANNKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPPFYYAYG 180

Query: 181 DGSLVARLYRDSLSLPTPAPSPPINVRNFTFGCAHTTLGEPVGVAGFGRGVLSMPSQLAT 240
           DGSLVARLYRDSLSLPTPAPSPPINVRNFTFGCAHTTLGEPVGVAGFGRGVLSMPSQLAT
Sbjct: 181 DGSLVARLYRDSLSLPTPAPSPPINVRNFTFGCAHTTLGEPVGVAGFGRGVLSMPSQLAT 240

Query: 241 FSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYHTGETEFIYTSLLENPKHPYFYSVGL 300
           FSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRY+TGETEFIYTSLLENPKHPYFYSVGL
Sbjct: 241 FSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYYTGETEFIYTSLLENPKHPYFYSVGL 300

Query: 301 AGISVGNVRIPAPEFLRKVDESGSGGVVVDSGTTFTMLPSGLYESVVAEFENRTGKVANR 360
           AGISVGN+RIPAPEFL KVDE GSGGVVVDSGTTFTMLP+GLYESVVAEFENRTGKVANR
Sbjct: 301 AGISVGNIRIPAPEFLTKVDEGGSGGVVVDSGTTFTMLPAGLYESVVAEFENRTGKVANR 360

Query: 361 ARRIEENTGLSPCYYYENSVGVPRVVLHFVGEKSSVVLPRKNYFYEFLDGGDGVVEVGRK 420
           ARRIEENTGLSPCYYYENSVGVPRVVLHFVGEKS+VVLPRKNYFYEFLDGGDGV  VGRK
Sbjct: 361 ARRIEENTGLSPCYYYENSVGVPRVVLHFVGEKSNVVLPRKNYFYEFLDGGDGV--VGRK 420

Query: 421 RKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDNLNR 480
           RKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDNLNR
Sbjct: 421 RKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDNLNR 479

Query: 481 S 482
           S
Sbjct: 481 S 479

BLAST of MELO3C013943 vs. NCBI nr
Match: gi|470130620|ref|XP_004301201.1| (PREDICTED: aspartic proteinase nepenthesin-2 [Fragaria vesca subsp. vesca])

HSP 1 Score: 666.0 bits (1717), Expect = 5.0e-188
Identity = 332/486 (68.31%), Postives = 385/486 (79.22%), Query Frame = 1

Query: 4   SPVFIFLLCFLLSSPVFSSQIFLLPLSHSLSSSISDFNSTHNLLKSTATRSSARFHRHRH 63
           SP+F+ +LCF   S  FS  +FL PL+HSLS +   FN+TH+LLK+TATRS+ RFHRHRH
Sbjct: 5   SPLFL-ILCFTYLSVSFSQTLFL-PLTHSLSQT--QFNTTHHLLKATATRSATRFHRHRH 64

Query: 64  N----HLSLPLSPGGDYTLSFNLGSES-HKISLYMDTGSDLVWFPCSPFECILCEGKPKI 123
                 +SLPLSPG DYTLSF LGS     ISLYMDTGSDLVWFPCSPFECILCEGKP  
Sbjct: 65  RKTTQQVSLPLSPGSDYTLSFTLGSSPPQSISLYMDTGSDLVWFPCSPFECILCEGKPNS 124

Query: 124 QSPLPKISNNKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPPFYYA 183
             P PKI  N +VSC + +CSAAH  +LS+  LCAI+ CPL+SIE+SECSSF CPPFYYA
Sbjct: 125 TFPPPKIPQNAAVSCDSHSCSAAHS-ALSSRSLCAIANCPLDSIELSECSSFKCPPFYYA 184

Query: 184 YGDGSLVARLYRDSLSLPTPAPSPPINVRNFTFGCAHTTLGEPVGVAGFGRGVLSMPSQL 243
           YGDGSL+++L+R SLS+P   PS  + + NFTFGC+H+ LGEP+GVAGFGRG+LS+P+QL
Sbjct: 185 YGDGSLISKLFRYSLSIPMSTPS--LLLPNFTFGCSHSALGEPIGVAGFGRGLLSLPAQL 244

Query: 244 ATFSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYHT----GETEFIYTSLLENPKHPY 303
           A  SP LGN+FSYCLVSHSF  +RV RPSPLILGRY      G  E+ YTS+L NPKHPY
Sbjct: 245 ARSSPHLGNQFSYCLVSHSFDQERVGRPSPLILGRYDQNSAHGADEYTYTSMLYNPKHPY 304

Query: 304 FYSVGLAGISVGNVRIPAPEFLRKVDESGSGGVVVDSGTTFTMLPSGLYESVVAEFENRT 363
           FY VGLAGIS+G   +PAPEFL++VDE G+GGVVVDSGTTFTMLP   Y S+VAEF+ R 
Sbjct: 305 FYCVGLAGISIGKRVVPAPEFLKRVDEKGNGGVVVDSGTTFTMLPQRFYNSLVAEFDRRV 364

Query: 364 GKVANRARRIEENTGLSPCYYYENSVGVPRVVLHFVGEKSSVVLPRKNYFYEFLDGGDGV 423
           G+V  RA ++E+ TGL PCYYY+  + VP V LHFVGEKSSVVLPRKNYFYEF DGGDG 
Sbjct: 365 GRVHKRATQVEDGTGLGPCYYYDGVMEVPAVTLHFVGEKSSVVLPRKNYFYEFTDGGDG- 424

Query: 424 VEVGRKRKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTL 481
              G+KRKVGC MLMNGGDE E  GGPGA  GNYQQQGFEVVYDLEK+RVGFA+RQCS L
Sbjct: 425 --TGKKRKVGCWMLMNGGDEKESGGGPGAIFGNYQQQGFEVVYDLEKHRVGFAKRQCSLL 480

BLAST of MELO3C013943 vs. NCBI nr
Match: gi|1009157128|ref|XP_015896606.1| (PREDICTED: aspartic proteinase nepenthesin-2 [Ziziphus jujuba])

HSP 1 Score: 661.4 bits (1705), Expect = 1.2e-186
Identity = 334/491 (68.02%), Postives = 391/491 (79.63%), Query Frame = 1

Query: 6   VFIFLLCFLLSS-PVFSSQIFLLPLSHSLSSSISDFNSTHNLLKSTATRSSARFHR---- 65
           ++  +LCF      V  SQI LLPL+HSLS +   FNST +LLKSTATRS+ARFHR    
Sbjct: 8   LYYIILCFSFECLSVSYSQILLLPLTHSLSQN--QFNSTQHLLKSTATRSAARFHRSRSD 67

Query: 66  -HRHNHLSLPLSPGGDYTLSFNLGSES-HKISLYMDTGSDLVWFPCSPFECILCEGK--P 125
            +RH+ +SLPLS G DYTLS  +G+     ISLYMDTGSDLVWFPCSPFECILCEGK  P
Sbjct: 68  RNRHSQVSLPLSSGSDYTLSLTVGTNPPQSISLYMDTGSDLVWFPCSPFECILCEGKYDP 127

Query: 126 KIQSPLPKISNNKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPPFY 185
           K  +   KI  N +VSC + ACSAAH  SLS+S+LCAI+RCPLESIEIS+CSSFSCPPFY
Sbjct: 128 KTTNKPLKIPPNATVSCKSPACSAAHS-SLSSSNLCAIARCPLESIEISDCSSFSCPPFY 187

Query: 186 YAYGDGSLVARLYRDSLSLPTPAPSPPINVRNFTFGCAHTTLGEPVGVAGFGRGVLSMPS 245
           YAY DGSL+ARL++  LS+P  +PS  + + NFTFGCAH+ LGEP+GVAGFGRG+LS+P+
Sbjct: 188 YAYADGSLIARLHKYRLSIPMSSPS--LVLHNFTFGCAHSALGEPIGVAGFGRGLLSLPA 247

Query: 246 QLATFSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYHTGE-------TEFIYTSLLEN 305
           QL++FSPQLGNRFSYCLVSHSF +DRVRRPSPLILGRY   E        +F+YTS+L+N
Sbjct: 248 QLSSFSPQLGNRFSYCLVSHSFDSDRVRRPSPLILGRYEEKEKRVGDDGAQFVYTSMLDN 307

Query: 306 PKHPYFYSVGLAGISVGNVRIPAPEFLRKVDESGSGGVVVDSGTTFTMLPSGLYESVVAE 365
           PKHPYFYSVGL GISVG   I APEFL  VD +G+GG+VVDSGTTFTMLPS LY S+VAE
Sbjct: 308 PKHPYFYSVGLVGISVGKKNILAPEFLHGVDATGNGGMVVDSGTTFTMLPSSLYNSLVAE 367

Query: 366 FENRTGKVANRARRIEENTGLSPCYYYENSVGVPRVVLHFVGEKSSVVLPRKNYFYEFLD 425
           F+ R G+V  RAR IE+ TGLSPCYYY   + +P + LHFVG +S V+LPR+NYFYEFLD
Sbjct: 368 FDQRVGRVHERARDIEDKTGLSPCYYYNKVIQIPNLTLHFVGNESGVLLPRRNYFYEFLD 427

Query: 426 GGDGVVEVGRKRKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARR 481
           GGDG    G+KR VGCLMLMNGGDE EL GGPGATLGNYQQQGFEVVYDL K RVGFARR
Sbjct: 428 GGDG---SGKKRNVGCLMLMNGGDEKELTGGPGATLGNYQQQGFEVVYDLAKRRVGFARR 487

BLAST of MELO3C013943 vs. NCBI nr
Match: gi|743864222|ref|XP_011031864.1| (PREDICTED: aspartic proteinase nepenthesin-1 [Populus euphratica])

HSP 1 Score: 661.0 bits (1704), Expect = 1.6e-186
Identity = 341/501 (68.06%), Postives = 390/501 (77.84%), Query Frame = 1

Query: 10  LLCFLLSSP---VFSSQIFLLPLSHSLSSSISDFNSTHNLLKSTATRSSARFHRHRHNH- 69
           LLCF+L      + +SQ   LPL HSLS +   F STH+LLKST+TRS+ARFH H H+H 
Sbjct: 8   LLCFILCFTHVFISTSQTLFLPLIHSLSKT--QFTSTHHLLKSTSTRSTARFHHHHHHHH 67

Query: 70  ----------LSLPLSPGGDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEGK 129
                     +SLPLSPG DYTLSF + S+   ISLY+DTGSDLVWFPC PFECILCEGK
Sbjct: 68  NNKNSHKHRQVSLPLSPGSDYTLSFTINSQP--ISLYLDTGSDLVWFPCQPFECILCEGK 127

Query: 130 PKIQS----PLPKISNNKS-VSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSF 189
            +  S    P PK+S   + VSC ++ACSA H  +L +S LCAIS CPLESIEIS+C   
Sbjct: 128 AENASLASTPPPKLSKTATPVSCKSSACSAVHS-NLPSSDLCAISNCPLESIEISDCRKH 187

Query: 190 SCPPFYYAYGDGSLVARLYRDSLSLPTPAPSPPINVRNFTFGCAHTTLGEPVGVAGFGRG 249
           SCP FYYAYGDGSL+ARLYRDS+ LP    +  I   NFTFGCAHTTL EP+GVAGFGRG
Sbjct: 188 SCPQFYYAYGDGSLIARLYRDSIRLPLSNQTNLI-FNNFTFGCAHTTLAEPIGVAGFGRG 247

Query: 250 VLSMPSQLATFSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYHTGETE---------- 309
           VLS+P+QLAT SPQLGN+FSYCLVSHSF +D VRRPSPLILGRY   E E          
Sbjct: 248 VLSLPAQLATLSPQLGNQFSYCLVSHSFDSDGVRRPSPLILGRYDHDEKERRVNGVKKPS 307

Query: 310 FIYTSLLENPKHPYFYSVGLAGISVGNVRIPAPEFLRKVDESGSGGVVVDSGTTFTMLPS 369
           F+YTS+L+NP+HPYFY VGL GIS+G  +IPAP+FLRKVD  GSGGVVVDSGTTFTMLP+
Sbjct: 308 FVYTSMLDNPRHPYFYCVGLEGISIGRKKIPAPDFLRKVDGEGSGGVVVDSGTTFTMLPA 367

Query: 370 GLYESVVAEFENRTGKVANRARRIEENTGLSPCYYYENS-VGVPRVVLHFVGEKSSVVLP 429
            LY+ +VAEFENR G+V  RA  IEENTGLSPCYY++N+ V VPRVVLHFVG  SSVVLP
Sbjct: 368 SLYDFIVAEFENRVGRVNERASVIEENTGLSPCYYFDNNVVNVPRVVLHFVGNGSSVVLP 427

Query: 430 RKNYFYEFLDGGDGVVEVGRKRKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDL 481
           R+NYFYEFLDGGDG    G+KRKVGCLMLMNGGDEAEL+GGPGATLGNYQQQGFEVVYDL
Sbjct: 428 RRNYFYEFLDGGDG---KGKKRKVGCLMLMNGGDEAELSGGPGATLGNYQQQGFEVVYDL 487

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
ASP63_ARATH3.7e-16762.71Probable aspartyl protease At4g16563 OS=Arabidopsis thaliana GN=At4g16563 PE=2 S... [more]
APF2_ARATH2.2e-3432.20Aspartyl protease family protein 2 OS=Arabidopsis thaliana GN=APF2 PE=2 SV=1[more]
ASPG1_ARATH3.1e-3330.02Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana GN=ASPG1 PE=1 ... [more]
NEP1_NEPGR7.0e-3329.34Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis GN=nep1 PE=1 SV=1[more]
NEP2_NEPGR4.5e-3228.75Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis GN=nep2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0L5I7_CUCSA4.0e-27797.92Pepsin A OS=Cucumis sativus GN=Csa_3G020060 PE=3 SV=1[more]
B9GYA7_POPTR1.5e-18668.47Aspartyl protease family protein OS=Populus trichocarpa GN=POPTR_0003s07390g PE=... [more]
B9NGC6_POPTR3.0e-18468.40Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0001s15870g PE=3 SV=1[more]
B9SSF8_RICCO1.5e-18366.40Pepsin A, putative OS=Ricinus communis GN=RCOM_1061010 PE=3 SV=1[more]
A0A061G3B8_THECC1.8e-18166.26Eukaryotic aspartyl protease family protein OS=Theobroma cacao GN=TCM_015978 PE=... [more]
Match NameE-valueIdentityDescription
AT4G16563.12.1e-16862.71 Eukaryotic aspartyl protease family protein[more]
AT5G45120.12.6e-5432.47 Eukaryotic aspartyl protease family protein[more]
AT3G52500.11.4e-4730.37 Eukaryotic aspartyl protease family protein[more]
AT3G61820.19.4e-3631.78 Eukaryotic aspartyl protease family protein[more]
AT1G01300.11.2e-3532.20 Eukaryotic aspartyl protease family protein[more]
Match NameE-valueIdentityDescription
gi|659095959|ref|XP_008448851.1|7.0e-283100.00PREDICTED: aspartic proteinase nepenthesin-1 [Cucumis melo][more]
gi|449458942|ref|XP_004147205.1|5.7e-27797.92PREDICTED: aspartic proteinase nepenthesin-1 [Cucumis sativus][more]
gi|470130620|ref|XP_004301201.1|5.0e-18868.31PREDICTED: aspartic proteinase nepenthesin-2 [Fragaria vesca subsp. vesca][more]
gi|1009157128|ref|XP_015896606.1|1.2e-18668.02PREDICTED: aspartic proteinase nepenthesin-2 [Ziziphus jujuba][more]
gi|743864222|ref|XP_011031864.1|1.6e-18668.06PREDICTED: aspartic proteinase nepenthesin-1 [Populus euphratica][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001461Aspartic_peptidase_A1
IPR001969Aspartic_peptidase_AS
IPR021109Peptidase_aspartic_dom_sf
Vocabulary: Molecular Function
TermDefinition
GO:0004190aspartic-type endopeptidase activity
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
biological_process GO:0030163 protein catabolic process
cellular_component GO:0005575 cellular_component
cellular_component GO:0009505 plant-type cell wall
molecular_function GO:0004190 aspartic-type endopeptidase activity
This gene is associated with the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
MU49151melon EST collection version 4.0transcribed_cluster

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO3C013943T1MELO3C013943T1mRNA


The following transcribed_cluster feature(s) are associated with this gene:

Feature NameUnique NameType
MU49151MU49151transcribed_cluster


Analysis Name: InterPro Annotations of melon
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001461Aspartic peptidase A1 familyPANTHERPTHR13683ASPARTYL PROTEASEScoord: 2..138
score: 9.7E-245coord: 163..479
score: 9.7E
IPR001969Aspartic peptidase, active sitePROSITEPS00141ASP_PROTEASEcoord: 327..338
scor
IPR021109Aspartic peptidase domainGENE3DG3DSA:2.40.70.10coord: 278..475
score: 5.5E-44coord: 73..272
score: 4.1
IPR021109Aspartic peptidase domainunknownSSF50630Acid proteasescoord: 71..476
score: 5.42
NoneNo IPR availablePANTHERPTHR13683:SF276SUBFAMILY NOT NAMEDcoord: 163..479
score: 9.7E-245coord: 2..138
score: 9.7E

The following gene(s) are paralogous to this gene:

None