MELO3C010142 (gene) Melon (DHL92) v3.5.1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	MELO3C010142
Type	gene
Organism	Cucumis melo (Melon (DHL92) v3.5.1)
Description	PIF1-like helicase
Location	chr2 : 13763123 .. 13763473 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATTGTCCCCACCCATTTGGAGGTAAAGTAATTGTCTTTGGAGGTGATTTTCGTCAAGTGCTTCCAGTGCTTCCTAAAGGAACAAGACAACAAACAATGGAAGTAGGTTTGAGGAAATCATATCTTTGAAAAAAAATGCACAAAATTCAGCTCAAGAAAAATATGAGAGCATATGACAATATAGGATTTGTAGATTTTATTTTGTGTGTTGGAGATGGAAACGAGCCCATCATCAAAGATGATATCATTAAATTGCTTGAGGAAATTGTAGTTGCAAATGATTCTGGAAATGATGCAAAAGGGATATTCATTAGGAAAATCTATCCTGATCTAGAACTACATACATGA

mRNA sequence

ATGGATTGTCCCCACCCATTTGGAGGTAAAGTAATTGTCTTTGGAGGTGATTTTCGTCAAGTGCTTCCAGTGCTTCCTAAAGGAACAAGACAACAAACAATGGAAGTAGATGGAAACGAGCCCATCATCAAAGATGATATCATTAAATTGCTTGAGGAAATTGTAGTTGCAAATGATTCTGGAAATGATGCAAAAGGGATATTCATTAGGAAAATCTATCCTGATCTAGAACTACATACATGA

Coding sequence (CDS)

ATGGATTGTCCCCACCCATTTGGAGGTAAAGTAATTGTCTTTGGAGGTGATTTTCGTCAAGTGCTTCCAGTGCTTCCTAAAGGAACAAGACAACAAACAATGGAAGTAGATGGAAACGAGCCCATCATCAAAGATGATATCATTAAATTGCTTGAGGAAATTGTAGTTGCAAATGATTCTGGAAATGATGCAAAAGGGATATTCATTAGGAAAATCTATCCTGATCTAGAACTACATACATGA

Protein sequence

MDCPHPFGGKVIVFGGDFRQVLPVLPKGTRQQTMEVDGNEPIIKDDIIKLLEEIVVANDSGNDAKGIFIRKIYPDLELHT*

Homology

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BLAST of MELO3C010142 vs. TrEMBL
Match: W5I9C8_WHEAT (Uncharacterized protein OS=Triticum aestivum PE=4 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 3.4e-11
Identity = 38/88 (43.18%), Postives = 53/88 (60.23%), Query Frame = 1


Query: 1   MDCPHPFGGKVIVFGGDFRQVLPVLPKGTRQQTMEV---------DGNEPIIKDDIIKLL 60
           M C  PFGGK+ VFGGDFRQVLPV+PKGTR Q   +         +G E  I DD ++L 
Sbjct: 100 MGCSLPFGGKIAVFGGDFRQVLPVVPKGTRAQANPLFSGYLLRIGNGTEETIGDDYVRLP 159

Query: 61  EEIVVANDSGNDAKGIFIRKIYPDLELH 80
           E+IV+ +     A    I++++P L ++
Sbjct: 160 EDIVIGHTDDETAINRLIKEVFPSLSVN 187

[Close]

BLAST of MELO3C010142 vs. TrEMBL
Match: W5HFA6_WHEAT (Uncharacterized protein OS=Triticum aestivum PE=4 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 4.9e-10
Identity = 37/85 (43.53%), Postives = 51/85 (60.00%), Query Frame = 1


Query: 1   MDCPHPFGGKVIVFGGDFRQVLPVLPKGTRQQT--------MEV-DGNEPIIKDDIIKLL 60
           M+CP PFGGKV+VFGGDFRQVLPV+ +GTR Q         + + +G E  I DD ++L 
Sbjct: 217 MECPLPFGGKVVVFGGDFRQVLPVVTRGTRAQADPWFSEYLLRIGNGTEETIGDDYVRLP 276

Query: 61  EEIVVANDSGNDAKGIFIRKIYPDL 77
           E+IV+      +     I  ++P L
Sbjct: 277 EDIVIGYTEDEEPINKLIEDVFPSL 301

[Close]

BLAST of MELO3C010142 vs. TrEMBL
Match: W4ZWT7_WHEAT (Uncharacterized protein OS=Triticum aestivum PE=4 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 1.4e-09
Identity = 37/85 (43.53%), Postives = 50/85 (58.82%), Query Frame = 1


Query: 1   MDCPHPFGGKVIVFGGDFRQVLPVLPKGTRQQT--------MEV-DGNEPIIKDDIIKLL 60
           MDC  PFGGKV+VFGGDFRQVLPV+ +GTR Q         + + +G E  I DD ++L 
Sbjct: 217 MDCSLPFGGKVVVFGGDFRQVLPVVTRGTRAQADPWFFEYLLRIGNGTEETIGDDYVRLP 276

Query: 61  EEIVVANDSGNDAKGIFIRKIYPDL 77
           ++IV+       A    I  ++P L
Sbjct: 277 DDIVIGYTEDEKAINTLIDNVFPSL 301

[Close]

BLAST of MELO3C010142 vs. TrEMBL
Match: W5FAI4_WHEAT (Uncharacterized protein OS=Triticum aestivum PE=4 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 5.4e-09
Identity = 38/85 (44.71%), Postives = 50/85 (58.82%), Query Frame = 1


Query: 1   MDCPHPFGGKVIVFGGDFRQVLPVLPKGTRQQT--------MEV-DGNEPIIKDDIIKLL 60
           M+CP PFGGKV+VFGGDFRQVL V+ +GTR Q         + + DG E  I DD  +L 
Sbjct: 219 MECPLPFGGKVVVFGGDFRQVLLVVTRGTRAQADPWFSEYLLRIGDGMEETIGDDYERLP 278

Query: 61  EEIVVANDSGNDAKGIFIRKIYPDL 77
           E+IV+A     +     I  ++P L
Sbjct: 279 EDIVIAYTEDEEPINKLIEDVFPSL 303

[Close]

BLAST of MELO3C010142 vs. TrEMBL
Match: C6JRR4_SORBI (Putative uncharacterized protein Sb0012s012570 OS=Sorghum bicolor GN=Sb0012s012570 PE=4 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 1.2e-08
Identity = 38/88 (43.18%), Postives = 53/88 (60.23%), Query Frame = 1


Query: 1   MDCPHPFGGKVIVFGGDFRQVLPVLPKGTRQQT--------MEV-DGNEPIIKDDIIKLL 60
           M+C  PFGGKV+VFGGDFRQVLPV+  GTR Q+        + + +G E  I DD ++L 
Sbjct: 227 MECALPFGGKVMVFGGDFRQVLPVVTCGTRAQSDPWFFEYLLRIGNGIEETIGDDYVRLP 286

Query: 61  EEIVVANDSGNDAKGIFIRKIYPDLELH 80
            +IV+     + A    I+ ++P LE H
Sbjct: 287 NDIVIGYTDTDVAVNKLIQHVFPALEEH 314

[Close]

BLAST of MELO3C010142 vs. NCBI nr
Match: gi|659109379|ref|XP_008454685.1| (PREDICTED: ATP-dependent DNA helicase PIF1-like [Cucumis melo])

HSP 1 Score: 122.1 bits (305), Expect = 4.5e-25
Identity = 66/99 (66.67%), Postives = 71/99 (71.72%), Query Frame = 1


Query: 1   MDCPHPFGGKVIVFGGDFRQVLPVLPKGTRQQTMEV--------------------DGNE 60
           MD  HPFGGK IVFGGDFRQ L VLPKGTRQQT+ +                    DGN+
Sbjct: 141 MDRLHPFGGKAIVFGGDFRQGLQVLPKGTRQQTVALKKNMRAYDDTRFADFILRVGDGNK 200

Query: 61  PIIKDDIIKLLEEIVVANDSGNDAKGIFIRKIYPDLELH 80
           PIIKDDIIKL E+IVVAN+S NDAKGIFIRKIYPDLELH
Sbjct: 201 PIIKDDIIKLPEDIVVANNSENDAKGIFIRKIYPDLELH 239

[Close]

BLAST of MELO3C010142 vs. NCBI nr
Match: gi|698466178|ref|XP_009782853.1| (PREDICTED: ATP-dependent DNA helicase pfh1-like [Nicotiana sylvestris])

HSP 1 Score: 74.7 bits (182), Expect = 8.2e-11
Identity = 39/76 (51.32%), Postives = 51/76 (67.11%), Query Frame = 1


Query: 1   MDCPHPFGGKVIVFGGDFRQVLPVLPKGTRQQTMEVDGNEPIIKDDIIKLLEEIVVANDS 60
           MD   PFGGKV+VFGGDFRQVLPV+PK TR +T  V+ +   IKDD+I L E++V+  + 
Sbjct: 80  MDIDEPFGGKVMVFGGDFRQVLPVVPKATRAET--VNASLHTIKDDMILLPEQLVIKPNG 139

Query: 61  GNDAKGIFIRKIYPDL 77
               +   I KI+P L
Sbjct: 140 NISGEDRLITKIFPSL 153

[Close]

BLAST of MELO3C010142 vs. NCBI nr
Match: gi|698489698|ref|XP_009791382.1| (PREDICTED: ATP-dependent DNA helicase PIF1-like [Nicotiana sylvestris])

HSP 1 Score: 74.3 bits (181), Expect = 1.1e-10
Identity = 41/113 (36.28%), Postives = 58/113 (51.33%), Query Frame = 1


Query: 1   MDCPHPFGGKVIVFGGDFRQVLPVLPKGTRQQTMEV------------------------ 60
           MD   PFGGKV+VFGGDFRQVLPV+PK TR +T+                          
Sbjct: 236 MDINEPFGGKVMVFGGDFRQVLPVVPKSTRVETINASLVKSYLWNKMEKIQLIRNMRARA 295

Query: 61  ------------DGNEPIIKDDIIKLLEEIVVANDSGNDAKGIFIRKIYPDLE 78
                       +G EP I+DD++ LLE+++V +D  +  +   IR+I+P L+
Sbjct: 296 DPTFSDFLLRIGNGEEPTIRDDMVLLLEKLIVKHDGDSTGEDNLIREIFPSLD 348

[Close]

BLAST of MELO3C010142 vs. NCBI nr
Match: gi|698528496|ref|XP_009761081.1| (PREDICTED: ATP-dependent DNA helicase pfh1-like [Nicotiana sylvestris])

HSP 1 Score: 70.5 bits (171), Expect = 1.6e-09
Identity = 41/113 (36.28%), Postives = 57/113 (50.44%), Query Frame = 1


Query: 1   MDCPHPFGGKVIVFGGDFRQVLPVLPKGTRQQTMEV------------------------ 60
           MD   PFGGKV+VFGGDFRQVLPV+PK TR +T+                          
Sbjct: 205 MDINEPFGGKVMVFGGDFRQVLPVVPKSTRVETINASLVKSYLWNKMEKIQLIRNMRARA 264

Query: 61  ------------DGNEPIIKDDIIKLLEEIVVANDSGNDAKGIFIRKIYPDLE 78
                       +G EP I+ D+I LLE+++V +D  +  +   IR+I+P L+
Sbjct: 265 DPTFSDFLLRIGNGEEPTIRYDMILLLEKLIVKHDGDSIGEDNLIREIFPSLD 317

[Close]

BLAST of MELO3C010142 vs. NCBI nr
Match: gi|697098387|ref|XP_009626438.1| (PREDICTED: uncharacterized protein LOC104117138 [Nicotiana tomentosiformis])

HSP 1 Score: 69.3 bits (168), Expect = 3.5e-09
Identity = 38/113 (33.63%), Postives = 57/113 (50.44%), Query Frame = 1


Query: 1   MDCPHPFGGKVIVFGGDFRQVLPVLPKGTRQQTMEV------------------------ 60
           MD   PF GKV+VFGGDFRQVLPV+PK TR +T+                          
Sbjct: 456 MDINEPFDGKVMVFGGDFRQVLPVVPKSTRAETINASLVTSYLGNKMEKIQLTRNMRARA 515

Query: 61  ------------DGNEPIIKDDIIKLLEEIVVANDSGNDAKGIFIRKIYPDLE 78
                       +G+EP I+DD++ L E++++ +D  +  +   IR+I+P L+
Sbjct: 516 DPTFNDFLQRIGNGDEPTIRDDMVLLPEKLIIKHDGDSTGEDNLIREIFPSLD 568

The following BLAST results are available for this feature:

BLAST of MELO3C010142 vs. TrEMBL


Match Name	E-value	Identity	Description
W5I9C8_WHEAT	3.4e-11	43.18	Uncharacterized protein OS=Triticum aestivum PE=4 SV=1	[more]
W5HFA6_WHEAT	4.9e-10	43.53	Uncharacterized protein OS=Triticum aestivum PE=4 SV=1	[more]
W4ZWT7_WHEAT	1.4e-09	43.53	Uncharacterized protein OS=Triticum aestivum PE=4 SV=1	[more]
W5FAI4_WHEAT	5.4e-09	44.71	Uncharacterized protein OS=Triticum aestivum PE=4 SV=1	[more]
C6JRR4_SORBI	1.2e-08	43.18	Putative uncharacterized protein Sb0012s012570 OS=Sorghum bicolor GN=Sb0012s0125...	[more]

BLAST of MELO3C010142 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659109379\|ref\|XP_008454685.1\|	4.5e-25	66.67	PREDICTED: ATP-dependent DNA helicase PIF1-like [Cucumis melo]	[more]
gi\|698466178\|ref\|XP_009782853.1\|	8.2e-11	51.32	PREDICTED: ATP-dependent DNA helicase pfh1-like [Nicotiana sylvestris]	[more]
gi\|698489698\|ref\|XP_009791382.1\|	1.1e-10	36.28	PREDICTED: ATP-dependent DNA helicase PIF1-like [Nicotiana sylvestris]	[more]
gi\|698528496\|ref\|XP_009761081.1\|	1.6e-09	36.28	PREDICTED: ATP-dependent DNA helicase pfh1-like [Nicotiana sylvestris]	[more]
gi\|697098387\|ref\|XP_009626438.1\|	3.5e-09	33.63	PREDICTED: uncharacterized protein LOC104117138 [Nicotiana tomentosiformis]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR010285	DNA_helicase_pif1-like

Vocabulary: **Biological Process**
Term	Definition
GO:0000723	telomere maintenance
GO:0006281	DNA repair

Vocabulary: **Molecular Function**
Term	Definition
GO:0003678	DNA helicase activity

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006281	DNA repair
biological_process	GO:0000723	telomere maintenance
cellular_component	GO:0005657	replication fork
molecular_function	GO:0003678	DNA helicase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MELO3C010142T1	MELO3C010142T1	mRNA

InterPro

Analysis Name: InterPro Annotations of melon
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR010285	DNA helicase Pif1-like	PFAM	PF05970	PIF1	coord: 3..36 score: 1.8
None	No IPR available	PANTHER	PTHR10492	UNCHARACTERIZED	coord: 5..77 score: 1.8
None	No IPR available	PANTHER	PTHR10492:SF32	SUBFAMILY NOT NAMED	coord: 5..77 score: 1.8

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
MELO3C010142	Cucumber (Chinese Long) v3	cucmeB044
MELO3C010142	Cucumber (Gy14) v1	cgymeB651
MELO3C010142	Cucurbita maxima (Rimu)	cmameB072
MELO3C010142	Cucurbita maxima (Rimu)	cmameB107
MELO3C010142	Cucurbita moschata (Rifu)	cmomeB065
MELO3C010142	Cucurbita moschata (Rifu)	cmomeB099
MELO3C010142	Wild cucumber (PI 183967)	cpimeB044
MELO3C010142	Cucumber (Chinese Long) v2	cumeB043
MELO3C010142	Watermelon (97103) v1	mewmB270
MELO3C010142	Bottle gourd (USVL1VR-Ls)	lsimeB398
MELO3C010142	Cucumber (Gy14) v2	cgybmeB040