MELO3C010117 (gene) Melon (DHL92) v3.5.1

BLAST of MELO3C010117 vs. Swiss-Prot
Match: FAB1B_ARATH (1-phosphatidylinositol-3-phosphate 5-kinase FAB1B OS=Arabidopsis thaliana GN=FAB1B PE=2 SV=1)

HSP 1 Score: 601.7 bits (1550), Expect = 2.0e-170
Identity = 358/765 (46.80%), Postives = 465/765 (60.78%), Query Frame = 1

Query: 595  NYRCHSCGMPSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGF 654
            +YRC SC MPSEAHVHCYTH QGSLTIS KKL D+ LPGE EGKIWMWHRCL+CPR NGF
Sbjct: 905  SYRCRSCEMPSEAHVHCYTHRQGSLTISVKKLQDYLLPGEKEGKIWMWHRCLRCPRLNGF 964

Query: 655  PPATRRVIMSDAAWGLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFH 714
            PPAT RV+MSDAAWGLSFGKFLELSFSN+AAASRVA CGH LHRDCLRFYGFG  VACF 
Sbjct: 965  PPATLRVVMSDAAWGLSFGKFLELSFSNHAAASRVACCGHSLHRDCLRFYGFGNMVACFR 1024

Query: 715  YAAINVQSVHIPPSMIEFHLNNLEWINKEANEVHNRAKTLFDEVCKALPLISKNESGAEQ 774
            YA I+V SV++PPS++ F+  N +WI +E +EV  RA+ LF EV  A+  I+  E G  +
Sbjct: 1025 YATIDVHSVYLPPSILSFNYENQDWIQRETDEVIERAELLFSEVLNAISQIA--EKGFRR 1084

Query: 775  EDFGADELNNYIQELEQIINMEKENFERLNYVATLRKKHSPDGSSNDISTLEEKELNSLE 834
                       I ELE+++  EK  FE  N    L ++ +      DI  L       L 
Sbjct: 1085 R----------IGELEEVLQKEKAEFEE-NMQKILHREVNEGQPLVDILELYRIHRQLLF 1144

Query: 835  ELV----ELTLASGRDEFFGGCD----NDSLPPESK-----------------REAGLGR 894
            +       L  AS   +     D     +  PP +K                  E  L  
Sbjct: 1145 QSYMWDHRLINASTLHKLENSDDTKREENEKPPLAKSQTLPEMNAGTNSLLTGSEVNLNP 1204

Query: 895  EGNS-GNISRLNETNRE--KNIDL------GGQEDDFCLSPVNSEPVDVGIGIRRANSVG 954
            +G+S G+   LN   +E   N DL      GG+       P  S P++  + +RR  S G
Sbjct: 1205 DGDSTGDTGSLNNVQKEADTNSDLYQEKDDGGEVSPSKTLPDTSYPLENKVDVRRTQSDG 1264

Query: 955  EYPIMTDLSGTLDAVWKG------VIHPNSLIFEDSFSRTNSAILEPLAG---QSDMEKC 1014
            +  +M +LS TLDA W G       I  N+ +     + +NS+   P++      D+ + 
Sbjct: 1265 QI-VMKNLSATLDAAWIGERQTSVEIPTNNKVSLPPSTMSNSSTFPPISEGLMPIDLPE- 1324

Query: 1015 TANGIETAHLLHSALVLTSDDSVETITNSTSMPPSAYNTKIS---LLNIPKSD-ISDYSS 1074
              N  + A+ +  AL   + ++ E   +  S+P   +   I+   LL+  K D   ++S 
Sbjct: 1325 QQNEFKVAYPVSPALPSKNYENSEDSVSWLSVPFLNFYRSINKNFLLSSQKLDTFGEHSP 1384

Query: 1075 VFISSYRELEKQSHLRLLMHVGSSDTVVPVYDEEPTSIIAYSLLMPEYIAQMSEPVKGME 1134
            ++ISS+RE E Q   RLL+ VG +D VVPVYD+EPTS+IAY+L+ PEY  Q S   + + 
Sbjct: 1385 IYISSFREAELQGGPRLLLPVGLNDIVVPVYDDEPTSMIAYALMSPEYQRQTSAEGESLV 1444

Query: 1135 ASDTARSLPVIGSVKMNSIKTCDEEGSDAYKTLRSNEETILSMPRTESLQFEDLVCMTKD 1194
            +  +  ++P          +  D+   D  ++  S +E+ILS+  + S    D +  TK 
Sbjct: 1445 SYPSELNIP----------RPVDDTIFDPSRSNGSVDESILSISSSRSTSLLDPLSYTKA 1504

Query: 1195 LHTRVCFVNETPLGPVKYTITCYFAKRFEALRKKCCPSELDFVRSLSRCRRWGAQGGKSN 1254
            LH RV +  +  LG VKYT+TCY+AKRFEALR  C PSEL+++RSLSRC++WGAQGGKSN
Sbjct: 1505 LHARVSYGEDGTLGKVKYTVTCYYAKRFEALRGICLPSELEYIRSLSRCKKWGAQGGKSN 1564

Query: 1255 VFFAKTLDERFIIKQVTKIELESFIQFSKAYFIYLSQAICTGCPTCLAKVVGMYQVSSKH 1313
            VFFAKTLD+RFIIKQVTK ELESFI+F+ AYF YLS++I T  PTCLAK++G+YQV++K 
Sbjct: 1565 VFFAKTLDDRFIIKQVTKTELESFIKFAPAYFKYLSESISTKSPTCLAKILGIYQVATKQ 1624

HSP 2 Score: 598.2 bits (1541), Expect = 2.2e-169
Identity = 326/615 (53.01%), Postives = 416/615 (67.64%), Query Frame = 1

Query: 9   SDLVGLVKSWMPWRSDPANVSRDFWMPDKSCRVCYECDSQFTFINRRHHCRRCGRVFCSK 68
           S++VGL+KSW+PWRS+PA VSRDFWMPD+SCRVCYECD QFT INRRHHCR CGRVFC K
Sbjct: 12  SEIVGLIKSWLPWRSEPATVSRDFWMPDQSCRVCYECDCQFTLINRRHHCRHCGRVFCGK 71

Query: 69  CTANFIPALSTDPTNAREDSEKIRVCNYCFKQWGKEPSSLQDGDKATSSA--LSVSSSST 128
           CTAN IP   +D    RED E+IRVCNYCF+QW +      DG    S+   LS S S T
Sbjct: 72  CTANSIPFAPSDLRTPREDWERIRVCNYCFRQWEQG-----DGGPHVSNITELSTSPSET 131

Query: 129 SMGSTKSGYTCHSAISHIASTPCS---TSRQYY--DPYCSAAIADEHDNLRNGKTINRTT 188
           S+ S+K+  T +S+   + S P       R ++  D       + E    + GK  +R +
Sbjct: 132 SLLSSKTSTTANSSSFALGSMPGLIGLNQRVHHGSDVSLHGVSSMETSVTKQGKETSRRS 191

Query: 189 SLMTS-----SSSYCGYYRSDDEDDDYYYGMYRSDSESRHISQSDDYYCAVNIDG-GLN- 248
           S + +     S       RSDDE D+Y  G Y++D E+ H  ++++YY  +  +G G++ 
Sbjct: 192 SFIATDVEDPSRFALNSIRSDDEYDEY--GAYQTDIETSHSPRANEYYGPMEYNGMGIDD 251

Query: 249 --------LERENTSTKGSPVNFDTQCP----RGNEESSEDSDENDKGSECDFPTYAMQN 308
                      +  S  GSP+    QC     R   E  +   E+D   EC+  + A  +
Sbjct: 252 VPCKHLGGETADQKSLSGSPLIH--QCLESLIREGSEQFQKKSEHDGRDECEASSPADIS 311

Query: 309 KDH--EPMDFQNNGILWLPPEPEDEEDEK--ALLFEDDDDVCAPGEWGHLHSSLS--SGE 368
            D   EP+DF+NNG+LW+PPEPE+EEDE+  AL  E+D++  A GEWG+L  S S  SGE
Sbjct: 312 DDQVVEPVDFENNGLLWVPPEPENEEDERESALFDEEDNEGDASGEWGYLRPSTSFGSGE 371

Query: 369 CPMKGKSSEDYKKTAKNVVEGHFRALVSQLLEAENLPIGDAPNEDGWLDIITYLSWEAAA 428
              + +++E++KK  KNVV+GHFRAL++QLL+ EN+ + D   ++ WL+IIT LSWEAA 
Sbjct: 372 YRGEDRTTEEHKKAMKNVVDGHFRALLAQLLQVENISVSDEEGKESWLEIITSLSWEAAN 431

Query: 429 VLKPDTSKSGGMDPGGYVKVKCVAGGKRSESIIVKGIICKKNVAHRRMTRGISKPRFFVL 488
           +LKPD SKSGGMDPGGYVKVKC+A G R +S++VKG++CKKNV +RRM+  I K R  +L
Sbjct: 432 LLKPDMSKSGGMDPGGYVKVKCLASGFRHDSMVVKGVVCKKNVVNRRMSTKIEKARLLIL 491

Query: 489 GGALEYQRVTNHLSSFDTLLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKN 548
           GG LEYQRV+N LSSFDTLLQQE DHLKMAVAKI A  PN+LLVEKSVSR AQEYLL+K+
Sbjct: 492 GGGLEYQRVSNQLSSFDTLLQQEKDHLKMAVAKIHAERPNILLVEKSVSRFAQEYLLAKD 551

Query: 549 ITLVLNIKRSLLERIACCTGANIIPTVDHVASLKLGYCDAFRVETFVEEHDSGDGQGGKK 592
           I+LVLNIKR LL+RIA CTGA IIP+VDH++S KLGYC+ FRV+ + EEH S  GQ GKK
Sbjct: 552 ISLVLNIKRPLLDRIARCTGAQIIPSVDHLSSQKLGYCENFRVDRYPEEHGS-TGQVGKK 611

BLAST of MELO3C010117 vs. Swiss-Prot
Match: FAB1A_ARATH (1-phosphatidylinositol-3-phosphate 5-kinase FAB1A OS=Arabidopsis thaliana GN=FAB1A PE=2 SV=1)

HSP 1 Score: 579.3 bits (1492), Expect = 1.0e-163
Identity = 337/759 (44.40%), Postives = 455/759 (59.95%), Query Frame = 1

Query: 598  CHSCGMPSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPA 657
            C SC MP+EAH+HCYTH QGSLTIS KKLP+  LPG+ EGKIWMWHRCLKCPR NGFPPA
Sbjct: 873  CPSCTMPAEAHIHCYTHRQGSLTISVKKLPEL-LPGQREGKIWMWHRCLKCPRINGFPPA 932

Query: 658  TRRVIMSDAAWGLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAA 717
            TRR++MSDAAWGLSFGKFLELSFSN+AAASRVA CGH LHRDCLRFYGFGR VACF YA+
Sbjct: 933  TRRIVMSDAAWGLSFGKFLELSFSNHAAASRVANCGHSLHRDCLRFYGFGRMVACFRYAS 992

Query: 718  INVQSVHIPPSMIEFHLNNLEWINKEANEVHNRAKTLFDEVCKALPLISKNESGAEQEDF 777
            IN+ +V +PP+ + F+  N EW+ KE+ EV  +A+ LF+EV +AL  IS    GA  +  
Sbjct: 993  INIYAVTLPPAKLYFNYENQEWLQKESKEVIKKAEVLFNEVQEALSQISAKTMGAGSKGS 1052

Query: 778  GADELNNYIQELEQIINMEKENF-ERLNYVATLRKKHSPDGSSNDISTLEEKELNSLEEL 837
              +++   ++EL  ++   K+ + + L  +  + K   P        T++   +N L  L
Sbjct: 1053 TPNKIKLSLEELAGLLEQRKKEYKDSLQQMLNVVKDGQP--------TIDILLINKLRRL 1112

Query: 838  VELTLASGRDEFFGGC-----DNDSLPPESKREAGLGREGNSGNISRLNETNREKNIDLG 897
            + +  +   DE   G      +N    P++     +GR  +   +S     +   ++ + 
Sbjct: 1113 I-IFDSYAWDECLAGAANMVRNNYLEAPKNSAPKVMGRNVSLEKLSDEKVKSIPTHVAIC 1172

Query: 898  GQEDDFCLSPVNSEPVDVGIGIRRANSVGEYPIMTDLSGTLDAVWKGVIHPNSLIFEDSF 957
               +D  L   + E   +  G   A++ G++ I  D+        +    P S   +D+F
Sbjct: 1173 ---NDSLLQDADYETC-LNQGKSFADTSGKFAIPEDVGSDRPPDCRMEFDP-SEGGKDNF 1232

Query: 958  SRTNSAILEPLAGQSDMEKCTANGIETAHLLHSALVLTSDDSV------ETITNSTSMPP 1017
               +S +++P   +S  +    +  +T          TS++ +         TN T +P 
Sbjct: 1233 VE-SSQVVKPAHTESQFQATDLS--DTLDAAWIGEQTTSENGIFRPPSRAASTNGTQIPD 1292

Query: 1018 ------------------SAYNTKISL--------------LNIPKSDISDYSSVFISSY 1077
                                + T++ L              LN  K  +++   V++SSY
Sbjct: 1293 LRLLGSESELNFKGGPTNDEHTTQVQLPSPSFYYSLNKNYSLNSRKHIMAEDRPVYVSSY 1352

Query: 1078 RELEKQSHLRLLMHVGSSDTVVPVYDEEPTSIIAYSLLMPEYIAQMSEPVKGMEASDTAR 1137
            RELE +S  RLL+ +G +D V+PVYD+EPTSIIAY+L   EY AQMS   K  +  D+  
Sbjct: 1353 RELEWRSGARLLLPLGCNDLVLPVYDDEPTSIIAYALTSSEYKAQMSGSDKSRDRLDSGG 1412

Query: 1138 SLPVIGSVKMNSIKTCDEEGSDAYKTLRSNEETILSMPRTESLQFEDLVCMTKDLHTRVC 1197
            S  +  SV + S+ +  +   D  ++L S +E +  +  +            KDLH R+ 
Sbjct: 1413 SFSLFDSVNLLSLNSLSDLSVDMSRSLSSADEQVSQLLHSS--------LYLKDLHARIS 1472

Query: 1198 FVNETPLGPVKYTITCYFAKRFEALRKKCCPSELDFVRSLSRCRRWGAQGGKSNVFFAKT 1257
            F +E P G VKY++TCY+AK FEALR  CCPSE DF+RSL RCR+WGAQGGKSNVFFAK+
Sbjct: 1473 FTDEGPPGKVKYSVTCYYAKEFEALRMICCPSETDFIRSLGRCRKWGAQGGKSNVFFAKS 1532

Query: 1258 LDERFIIKQVTKIELESFIQFSKAYFIYLSQAICTGCPTCLAKVVGMYQVSSKHLKGGKE 1313
            LD+RFIIKQVTK ELESFI+F  AYF YL+++I T  PT LAK++G+YQVSSKHLKGGKE
Sbjct: 1533 LDDRFIIKQVTKTELESFIKFGPAYFKYLTESISTKSPTSLAKILGIYQVSSKHLKGGKE 1592

HSP 2 Score: 575.9 bits (1483), Expect = 1.2e-162
Identity = 316/624 (50.64%), Postives = 408/624 (65.38%), Query Frame = 1

Query: 1   MGHPENKISDLVGLVKSWMPWRSDPANVSRDFWMPDKSCRVCYECDSQFTFINRRHHCRR 60
           M   ++K    V +VKSW+P +S+ +N+SRDFWMPD+SC VCYECD+QFT  NRRHHCR 
Sbjct: 1   MDSQDHKAPGFVDIVKSWIPRKSESSNMSRDFWMPDQSCPVCYECDAQFTVFNRRHHCRL 60

Query: 61  CGRVFCSKCTANFIPALSTDPTNAREDSEKIRVCNYCFKQWGKEPSSLQDGDKATSSALS 120
           CGRVFC+KC AN IP+ S +  ++ E+ E+IRVCNYC+KQW +      +G    S   S
Sbjct: 61  CGRVFCAKCAANSIPSPSDETKDSHEEPERIRVCNYCYKQWEQGIVPPDNGASIISLHFS 120

Query: 121 VSSSSTSMGSTKSGYTCHSAISHIASTPCSTSRQYYDPYCSAAIAD-------EHDNLRN 180
            S S+ S+ ST    T +S+   I ST   + R   +P  S  ++        E  N ++
Sbjct: 121 SSPSARSVAST----TSNSSNCTIDSTAGPSPRPKMNPRASRRVSSNMDSEKSEQQNAKS 180

Query: 181 GKTINRTTSLMTSSSSYCGYY-----RSD---DEDDDYYYGMYRSDSESRHISQSDDYYC 240
            ++ +    ++ SS +   ++     RSD   D+DDDY     +S       +Q +DYY 
Sbjct: 181 RRSSDHYGHVLDSSDNQVEFFVNSSGRSDGEADDDDDYQSDFAQS------YAQGNDYYG 240

Query: 241 AVNIDG-------------GLNLERENTSTKGSPVNFDTQCPRGNEESSEDSDENDKGSE 300
           A+N+D              G+ +E    +  G P + D               EN     
Sbjct: 241 AINLDEVDHIYGSHEAHDVGVKIE---PNISGFPPDQDLDSLNTETIDKTRQQENGWNDV 300

Query: 301 CDFPTYAMQNKDHEPMDFQNNGILWLPPEPEDEEDEK-ALLFEDDDDVCAPGEWGHLHSS 360
            +      ++ + E +DF+++G+LWLPPEPE+EEDE+ A+L +DD D    G+WG+L  S
Sbjct: 301 KEGSPPCEESFEPEVVDFESDGLLWLPPEPENEEDEREAVLSDDDGDEGDRGDWGYLRPS 360

Query: 361 LSSGECPMKGKSSEDYKKTA-KNVVEGHFRALVSQLLEAENLPIGDAPNEDGWLDIITYL 420
            S  E   K   S+D    A KNVVEGHFRALV+QLLE +NLP+ +  +E+GWLDIIT L
Sbjct: 361 NSFNE---KDFHSKDKSSGAMKNVVEGHFRALVAQLLEVDNLPMVNEGDEEGWLDIITSL 420

Query: 421 SWEAAAVLKPDTSKSGGMDPGGYVKVKCVAGGKRSESIIVKGIICKKNVAHRRMTRGISK 480
           SWEAA +LKPDTSKSGGMDPGGYVKVKC+  G+RSES++VKG++CKKNVAHRRMT  I K
Sbjct: 421 SWEAATLLKPDTSKSGGMDPGGYVKVKCIPCGRRSESMVVKGVVCKKNVAHRRMTSKIEK 480

Query: 481 PRFFVLGGALEYQRVTNHLSSFDTLLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQE 540
           PR  +LGGALEYQR++N LSSFDTLLQQE+DHLKMAVAKID+H+P++LLVEKSVSR AQE
Sbjct: 481 PRLLILGGALEYQRISNQLSSFDTLLQQEMDHLKMAVAKIDSHNPDILLVEKSVSRFAQE 540

Query: 541 YLLSKNITLVLNIKRSLLERIACCTGANIIPTVDHVASLKLGYCDAFRVETFVEEHDSGD 595
           YLL+K+I+LVLNIKRSLLERI+ CTGA I+P++D + S KLGYCD F VE FVE H S  
Sbjct: 541 YLLAKDISLVLNIKRSLLERISRCTGAQIVPSIDQLTSPKLGYCDLFHVEKFVETHVS-P 600

BLAST of MELO3C010117 vs. Swiss-Prot
Match: FAB1C_ARATH (Putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1C OS=Arabidopsis thaliana GN=FAB1C PE=2 SV=1)

HSP 1 Score: 451.4 bits (1160), Expect = 3.3e-125
Identity = 285/744 (38.31%), Postives = 411/744 (55.24%), Query Frame = 1

Query: 598  CHSCGMPSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPA 657
            C SC    +AHV CY+H  G+LTI+ ++LP   LPGE +GKIWMWHRCL+C   +G PPA
Sbjct: 825  CRSCKELVDAHVLCYSHQNGNLTINVRRLPSMKLPGEQDGKIWMWHRCLRCAHVDGVPPA 884

Query: 658  TRRVIMSDAAWGLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAA 717
            TRRV+MSDAAWGLSFGKFLELSFSN+A A+RVA CGH L RDCLRFYGFG  VA F Y+ 
Sbjct: 885  TRRVVMSDAAWGLSFGKFLELSFSNHATANRVASCGHSLQRDCLRFYGFGNMVAFFRYSP 944

Query: 718  INVQSVHIPPSMIEFHLN-NLEWINKEANEVHNRAKTLFDEVCKALPLISKNESGAEQED 777
            IN+ +V +PPSM+EF+ +   EWI  EA E+  + +T++ E+   L  + +  S  E E 
Sbjct: 945  INILTVLLPPSMLEFNSHPQQEWIRTEAAELVGKMRTMYTEISDMLNRMEEKSSLLEPEQ 1004

Query: 778  FGADELNNYIQELEQIINMEKENFERLNYVATLRKKHSPDGSSNDISTLEEKELNSLEEL 837
              A +L++ I  L   +  EK+ ++  + +  + +++     S DI      ELN L   
Sbjct: 1005 SEACDLHSRIIGLIDQLVKEKDEYD--DALQPIFEENLQIQGSLDIL-----ELNRLRRA 1064

Query: 838  V---------ELTLASGR----DEFFGGCDNDSLPPESKREAGLGREGNSGNISR--LNE 897
            +         +L L + +      F  G DN    PE      + R    G+  R   + 
Sbjct: 1065 LMIGAHAWDHQLYLLNSQLKKASVFKTGDDNAPRNPEMHDPPKIDRRMQEGSDERDEQSH 1124

Query: 898  TNREKNIDLGGQEDDFCLSPVNSEPVDVGIGIRRANSVGEYPIMTDLSGTLDAVWKGVIH 957
            T+ E N D             N +P ++        S G     T LS  +D+ W G   
Sbjct: 1125 TDSEANGD-------------NKDPENIP-------SPG-----TSLSERIDSAWLGSFQ 1184

Query: 958  ----PNSLIFEDSFSRTNSAI---LEPLAGQS-----DMEKCTANGIETAHLLHSAL--V 1017
                  ++   + FS  NS++     P+  QS       ++    G+  + L  S L   
Sbjct: 1185 NLEKAETIAETEGFSAVNSSLRRLARPIRVQSFDSAIRFQERIQKGLPPSSLYLSTLRSF 1244

Query: 1018 LTSDDSVETITNSTSMPPSAYNTKISLLNIPKSDISDYSS-VFISSYRELEKQSHLRLLM 1077
              S +    + +  S     Y+  + L  + K D+   S+  +ISS  ++   + + L+ 
Sbjct: 1245 HASGEYRNMVRDPVSNVMRTYSQMLPL-EVQKLDLIVGSAPTYISSASQMADGARM-LIP 1304

Query: 1078 HVGSSDTVVPVYDEEPTSIIAYSLLMPEYIAQMSEPVKGMEASDTARSLPVIGSVKMNSI 1137
              G +D VVPVYD++P S+++Y++   EY   +    KG+ +S ++ +L         + 
Sbjct: 1305 QRGLNDIVVPVYDDDPASVVSYAINSKEYKEWIVN--KGLASSSSSSNL---------NN 1364

Query: 1138 KTCDEEGSDAYKTLRSNEETILSMPRTESLQFEDLVCMTKDLHTRVCFVNETPLGPVKYT 1197
            +  +      +++L  + + I       S        +T     R    +    G VK++
Sbjct: 1365 RESEPSAFSTWRSLSMDVDYIQHAVYGSSQDDRKSPHLTISFSDRASSSSTATEGKVKFS 1424

Query: 1198 ITCYFAKRFEALRKKCCPSELDFVRSLSRCRRWGAQGGKSNVFFAKTLDERFIIKQVTKI 1257
            +TCYFA +F+ LRK CCPSE+DFVRSLSRC+RW AQGGKSNV+FAK+LDERFIIKQV K 
Sbjct: 1425 VTCYFATQFDTLRKTCCPSEVDFVRSLSRCQRWSAQGGKSNVYFAKSLDERFIIKQVVKT 1484

Query: 1258 ELESFIQFSKAYFIYLSQAICTGCPTCLAKVVGMYQVSSKHLKGGKECKVDVLVMENLLF 1311
            EL+SF  F+  YF YL +++ +G PTCLAK++G+YQVS KH KGGKE K+D++VMENL +
Sbjct: 1485 ELDSFEDFAPEYFKYLKESLSSGSPTCLAKILGIYQVSIKHPKGGKETKMDLMVMENLFY 1523

HSP 2 Score: 325.9 bits (834), Expect = 2.1e-87
Identity = 183/433 (42.26%), Postives = 253/433 (58.43%), Query Frame = 1

Query: 190 YCGYYRSDDEDDDYYYGMYRSDSESRHISQSDDYYCAVNIDGGLNLE---RENTSTKGSP 249
           YCG        D+Y      SD ES  +S   + +   +  G    +   R N S  G  
Sbjct: 148 YCGKQLLSPSSDNYQDS---SDIESGSVSARHELFSCKSSAGSSPHDSPLRNNFSPLGRF 207

Query: 250 VNF------DTQCPRGNEESSEDSDENDKGSECDFPTYAMQNKDHE--------PMDFQN 309
           V         T C   N +    +D   K  +       ++ +DHE        P+DF+N
Sbjct: 208 VQHAKDLRSPTVCSFDNHQEQLLADNLVKPGQG-----VLEQEDHEEEEDKLQQPLDFEN 267

Query: 310 NGILWLPPEPEDEEDEKALLF----EDDDDVCAPGEWGHLHSSLSSGECPMKGKSSEDYK 369
           NG +W PP PEDE D+    +    ++DDD+        L SS SS   P K K  E+  
Sbjct: 268 NGRIWYPPPPEDENDDAESNYFHYDDEDDDIGDSATEFSLSSSFSS-HIPTKEKLGENSN 327

Query: 370 KTAKNVVEGHFRALVSQLLEAENLPIGDAPNEDGWLDIITYLSWEAAAVLKPDTSKSGGM 429
           +  + VV  HFRALV++LL  E L   D  +   WLDI+T L+W+AA  +KPDT   G M
Sbjct: 328 EPLRTVVHDHFRALVAELLRGEELSPSDDGSAGEWLDIVTALAWQAANFVKPDTRAGGSM 387

Query: 430 DPGGYVKVKCVAGGKRSESIIVKGIICKKNVAHRRMTRGISKPRFFVLGGALEYQRVTNH 489
           DPG YVK+KCVA G ++ESI+++GI+C KN+ H+RM      PR  +L G+LEYQRV   
Sbjct: 388 DPGNYVKIKCVASGNQNESILIRGIVCSKNITHKRMISQYKNPRVMLLAGSLEYQRVAGQ 447

Query: 490 LSSFDTLLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLL 549
           L+SF+TLLQQE +H+K  +AKI++  PNVLLVEKS S +AQ+YLL K I+LVLN+KRSLL
Sbjct: 448 LASFNTLLQQENEHMKAIIAKIESLRPNVLLVEKSASSYAQQYLLEKEISLVLNVKRSLL 507

Query: 550 ERIACCTGANIIPTVDHVASLKLGYCDAFRVETFVEEHDSGDGQGGKKSTKTLMFFEGCP 602
           +RIA CTGA + P++D +++ +LG+C+ FR E  +E+H++G+ Q  +K ++TLM+FEGCP
Sbjct: 508 DRIARCTGAVLCPSLDSISTARLGHCELFRTERVLEQHEAGN-QSNRKPSRTLMYFEGCP 567

BLAST of MELO3C010117 vs. Swiss-Prot
Match: FAB1D_ARATH (Putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1D OS=Arabidopsis thaliana GN=FAB1D PE=3 SV=1)

HSP 1 Score: 201.8 bits (512), Expect = 4.5e-50
Identity = 144/398 (36.18%), Postives = 208/398 (52.26%), Query Frame = 1

Query: 211 DSESRHISQSDDYYCAVNIDGGLNLERENTSTKGSPVNFDTQCPRGNEESSEDSDENDKG 270
           D  S H SQ D     V +   + ++R    +K  P +        +E+S E+S EN   
Sbjct: 31  DECSSHSSQED-----VELTKEVKVDRLERKSKSMPSDILDIL---DEKSKENSVEN--- 90

Query: 271 SECDFPTYAMQNKDHEPMDFQNNGILWLPPEPEDEEDEKALLFEDDDDVCAPGEWGHLHS 330
               F +    + D  P+        W PPEPE+ EDE   +F DDDD C  G   +  S
Sbjct: 91  --VQFLSDREDDSDDVPV--------WEPPEPENPEDEVDGVFADDDDDCCDGSKWNKAS 150

Query: 331 SLS--SGECPMKGKSSEDYKKTAKNVVEGHFRALVSQLLEAENLPIGDAPNEDG-WLDII 390
            L   S E   K K  E+ ++      +  F+ +VSQL+++    I     E G W +I+
Sbjct: 151 LLGELSDESSEKRKVYEENRRVMLEEADSKFKFIVSQLIKSAGFSI----EESGYWFEIV 210

Query: 391 TYLSWEAAAVLKPDTSKSGGMDPGGYVKVKCVAGGKRSESIIVKGIICKKNVAHRRMTRG 450
             L WEAA++LKP       +DP  Y+KVKC+A G   +S + KG++ KK+ A + M   
Sbjct: 211 ARLCWEAASMLKPAIDGKS-VDPTEYIKVKCIATGSCVDSEVFKGLVFKKHAALKHMATK 270

Query: 451 ISKPRFFVLGGALEYQRVTNHLSSFDTLLQQE---LDHLKMAVAKIDAHHPNVLLVEKSV 510
              PR  ++ G L +    +  SS  ++ Q     L ++K  V  I+A  P+V+LVEKSV
Sbjct: 271 YEHPRIMLVEGVLGHP--ISGFSSLQSVNQDNEYLLKYVKPVVDIIEASKPDVMLVEKSV 330

Query: 511 SRHAQEYLLSKNITLVLNIKRSLLERIACCTGANIIPTVDHVASLKLGYCDAFRVETFVE 570
           SR  Q+ +L K +TLV ++K   L+RI+ C G+ I+ +VD ++S KL +CD+FR+E  VE
Sbjct: 331 SRDIQKTILDKGVTLVFDMKLHRLQRISRCIGSPIL-SVDSLSSQKLKHCDSFRIEKIVE 390

Query: 571 EHDSGDGQGGKKSTKTLMFFEGCPKPLCCTNY--RCHS 601
           EH++  G+  KK TKTLMF EGCP  L CT     CHS
Sbjct: 391 EHNAA-GESDKKPTKTLMFLEGCPTRLGCTILLKGCHS 398

HSP 2 Score: 163.3 bits (412), Expect = 1.8e-38
Identity = 102/284 (35.92%), Postives = 161/284 (56.69%), Query Frame = 1

Query: 1022 ISDYSSVFISSYRELEKQSHLRLLMHVGSSDTVVPVYDEEPTSIIAYSLLMPEYIAQMSE 1081
            +++++   + +  ++  +   RL + +   D +V  Y++E +S+IA +L      A ++ 
Sbjct: 1063 VNNFTQENLQTVNQIITEESSRLRISLRDDDFIVSDYEDELSSLIACAL------AHLNN 1122

Query: 1082 PVKGMEASDTARSLPVIGSVKMNSIKTCDEEGSDAYKTLRSNEETILSMPRTESLQFEDL 1141
                 E S    S  + GS++       D + +D   +  S+E T     R E+L   ++
Sbjct: 1123 -----EESKKPLSRCIHGSLQGFLDNNQDSKQTDRDVSRFSSEST----NRLETLPPPEV 1182

Query: 1142 VCMTKDLHTRVCFVNETPLGPVKYTITCYFAKRFEALRKKCCPSELDFVRSLSRCRRWGA 1201
            +         V F +   +G  KY+I   +A  F  LRK+CC SELD++ SLSRC+ W A
Sbjct: 1183 L---------VTFGSVKSVGKPKYSIVSLYADDFRDLRKRCCSSELDYIASLSRCKPWDA 1242

Query: 1202 QGGKSNVFFAKTLDERFIIKQVTKIELESFIQFSKAYFIYLSQAICTGCPTCLAKVVGMY 1261
            +GGKS   FAKTLD+RFI+K++ K E ESF+ F+  YF Y+  +   G  TCLAKV+G++
Sbjct: 1243 KGGKSKSVFAKTLDDRFIVKEIKKTEYESFVTFATEYFKYMKDSYDLGNQTCLAKVLGIH 1302

Query: 1262 QVSSKHLK-GGKECKVDVLVMENLLFRHNVTRLYDLKGSSRSRY 1305
            QV+ +  K GGKE + D++VMENL F   VTR YDLKG+  +R+
Sbjct: 1303 QVTVRQPKGGGKEIRHDLMVMENLSFSRKVTRQYDLKGALHARF 1322

HSP 3 Score: 129.8 bits (325), Expect = 2.2e-28
Identity = 74/206 (35.92%), Postives = 103/206 (50.00%), Query Frame = 1

Query: 598 CHSCGMPSEAHVHCYTHPQGSLTISAKKLPDFF-LPGEPEGKIWMWHRCLKCPRTNGFPP 657
           C +C    EAH++ Y H    LTI  K++P    L GE +GKIWMW RC KC   N    
Sbjct: 647 CQTCVEFPEAHLYYYAHQNKQLTIQIKRIPVAKGLAGEAKGKIWMWSRCGKCKTKNASRK 706

Query: 658 ATRRVIMSDAAWGLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYA 717
           +T+RV++S AA  LSFGKFLELSFS     +R + CGH    D L F+G G  VA   Y+
Sbjct: 707 STKRVLISTAARSLSFGKFLELSFSQQTFLNRSSSCGHSFDSDFLHFFGLGSMVAMLSYS 766

Query: 718 AINVQSVHIPPSMIEFH-LNNLEWINKEANEVHNRAKTLFDEVCKALPLISKNESGAEQE 777
            +   +V +PP  +E   L    W+ KE   V  +  +LF++   A   + +  S     
Sbjct: 767 QVASYTVSLPPMKLESSILIKAGWLEKEFQTVFTKGISLFED---AAGFLKRLRSQFTNS 826

Query: 778 DFGADELNNYIQELEQIINMEKENFE 802
           D         +  +E+++  E+  FE
Sbjct: 827 DLRYQRARKLLSNIEELLKHERCIFE 849

BLAST of MELO3C010117 vs. Swiss-Prot
Match: FYV1_HUMAN (1-phosphatidylinositol 3-phosphate 5-kinase OS=Homo sapiens GN=PIKFYVE PE=1 SV=3)

HSP 1 Score: 146.7 bits (369), Expect = 1.7e-33
Identity = 87/240 (36.25%), Postives = 134/240 (55.83%), Query Frame = 1

Query: 358 HFRALVSQLLEAENLPIGDAPNEDGWLDIITYLSWEAAAVLKPDT-SKSGGMDPGGYVKV 417
           H  AL+ QLL +++L          W DII  L  +    ++PD  ++   MD   +V +
Sbjct: 615 HMMALLQQLLHSDSL-------SSSWRDIIVSLVCQVVQTVRPDVKNQDDDMDIRQFVHI 674

Query: 418 KCVAGGKRSESIIVKGIICKKNVAHRRMTRGISKPRFFVLGGALEY-QRVTNHLSSFDTL 477
           K + GGK+ +S++V G +C KN+AH++M+  I  P+  +L  ++EY  R     +  D +
Sbjct: 675 KKIPGGKKFDSVVVNGFVCTKNIAHKKMSSCIKNPKILLLKCSIEYLYREETKFTCIDPI 734

Query: 478 LQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCT 537
           + QE + LK  V +I    P ++LVEK+VSR AQ+ LL   ITLV+N+K  +LERI+  T
Sbjct: 735 VLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMT 794

Query: 538 GANIIPTVDHVASL-KLGYCDAFRVETFVEEHDSGDGQGGKKSTKTLMFFEGCPKPLCCT 595
             +++ ++D + +   LG C  F ++ F         Q   + TKTLMFFEGCP+ L CT
Sbjct: 795 QGDLVMSMDQLLTKPHLGTCHKFYMQIF---------QLPNEQTKTLMFFEGCPQHLGCT 838

HSP 2 Score: 120.6 bits (301), Expect = 1.3e-25
Identity = 61/149 (40.94%), Postives = 93/149 (62.42%), Query Frame = 1

Query: 1164 KYTITCYFAKRFEALRKKCCPS-ELDFVRSLSRCRRWGAQGGKSNVFFAKTLDERFIIKQ 1223
            K+    Y+A  F  +R+    S E DF+RSLS    W A+GGKS   F  T D+RFI+KQ
Sbjct: 1819 KFYCRLYYAGEFHKMREVILDSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQ 1878

Query: 1224 VTKIELESFIQFSKAYFIYLSQAICTGCPTCLAKVVGMYQVSSKHLKGGKECKVDVLVME 1283
            + ++E++SF+ F+  YF Y++ A+    PT LAK++G+Y++  K+ +   E K+D+LVME
Sbjct: 1879 MPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVME 1938

Query: 1284 NLLFRHNVTRLYDLKGSSRSRYNPDTSGK 1312
            NL +   + +++DLKGS R+R     +GK
Sbjct: 1939 NLFYGRKMAQVFDLKGSLRNRNVKTDTGK 1967

HSP 3 Score: 79.3 bits (194), Expect = 3.4e-13
Identity = 44/138 (31.88%), Postives = 66/138 (47.83%), Query Frame = 1

Query: 8   ISDLVGLVKSWMPWRSDPANVSRDFWMPDKSCRVCYECDSQFTFINRRHHCRRCGRVFCS 67
           +S ++  +K  M  +S  +++ + +WMPD  C+ CY+C  +FT   RRHHCR CG++FCS
Sbjct: 131 LSTVLKRLKEIMEGKSQDSDL-KQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCS 190

Query: 68  KCTANFIPALSTDPTNAREDSEKIRVCNYCFK------------QWGKEPSSLQDGDKAT 127
           +C    IP      T        +R C YC K              G++ ++L D    +
Sbjct: 191 RCCNQEIPGKFMGYTG------DLRACTYCRKIALSYAHSTDSNSIGEDLNALSD-SACS 250

Query: 128 SSALSVSSSSTSMGSTKS 134
            S L  S   T +GS K+
Sbjct: 251 VSVLDPSEPRTPVGSRKA 260

BLAST of MELO3C010117 vs. TrEMBL
Match: A0A0A0M3A5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G662800 PE=4 SV=1)

HSP 1 Score: 1208.4 bits (3125), Expect = 0.0e+00
Identity = 579/594 (97.47%), Postives = 585/594 (98.48%), Query Frame = 1

Query: 1   MGHPENKISDLVGLVKSWMPWRSDPANVSRDFWMPDKSCRVCYECDSQFTFINRRHHCRR 60
           MGHPENKISDLVGLVKSWMPWRSDP NVSRDFWMPDKSCRVCYECDSQFTFINRRHHCRR
Sbjct: 1   MGHPENKISDLVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYECDSQFTFINRRHHCRR 60

Query: 61  CGRVFCSKCTANFIPALSTDPTNAREDSEKIRVCNYCFKQWGKEPSSLQDGDKATSSALS 120
           CGRVFCS+CTANFIPA STD TNAREDSEKIRVCNYCFKQWGKEPSSLQDGDKATSSALS
Sbjct: 61  CGRVFCSRCTANFIPAPSTDATNAREDSEKIRVCNYCFKQWGKEPSSLQDGDKATSSALS 120

Query: 121 VSSSSTSMGSTKSGYTCHSAISHIASTPCSTSRQYYDPYCSAAIADEHDNLRNGKTINRT 180
           +SSSSTSMGSTKSGYTCHSAISHIASTPCST RQYYDPYCSAA+ADEHDNLRNGKTINRT
Sbjct: 121 LSSSSTSMGSTKSGYTCHSAISHIASTPCSTGRQYYDPYCSAAMADEHDNLRNGKTINRT 180

Query: 181 TSLMTSSSSYCGYYRSDDEDDDYYYGMYRSDSESRHISQSDDYYCAVNIDGGLNLERENT 240
           TSLMTSSSSY GYYRSDDEDDDYYYGMYRSDSESRHISQSDDYYCAVNID G NLERENT
Sbjct: 181 TSLMTSSSSYYGYYRSDDEDDDYYYGMYRSDSESRHISQSDDYYCAVNIDEGSNLERENT 240

Query: 241 STKGSPVNFDTQCPRGNEESSEDSDENDKGSECDFPTYAMQNKDHEPMDFQNNGILWLPP 300
           STKGSPVNFDTQ PRGNEESSEDSDENDKGSECDFP+YAMQNK+HEPMDFQNNGILWLPP
Sbjct: 241 STKGSPVNFDTQSPRGNEESSEDSDENDKGSECDFPSYAMQNKNHEPMDFQNNGILWLPP 300

Query: 301 EPEDEEDEKALLFEDDDDVCAPGEWGHLHSSLSSGECPMKGKSSEDYKKTAKNVVEGHFR 360
           EPEDEEDEKALLFEDDDDVCAPGEWG+LHSSLSSGECPMKGKSSEDYKKTAKNVVEGHFR
Sbjct: 301 EPEDEEDEKALLFEDDDDVCAPGEWGYLHSSLSSGECPMKGKSSEDYKKTAKNVVEGHFR 360

Query: 361 ALVSQLLEAENLPIGDAPNEDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYVKVKCVA 420
           ALVSQLLEAENLPIGDAP EDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYVKVKCVA
Sbjct: 361 ALVSQLLEAENLPIGDAPREDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYVKVKCVA 420

Query: 421 GGKRSESIIVKGIICKKNVAHRRMTRGISKPRFFVLGGALEYQRVTNHLSSFDTLLQQEL 480
           GGKRSESIIVKGIICKKNVAHRRMTRGISKPRFFVLGGALEYQRVTNHLSSFDTLLQQEL
Sbjct: 421 GGKRSESIIVKGIICKKNVAHRRMTRGISKPRFFVLGGALEYQRVTNHLSSFDTLLQQEL 480

Query: 481 DHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCTGANII 540
           DHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCTGANII
Sbjct: 481 DHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCTGANII 540

Query: 541 PTVDHVASLKLGYCDAFRVETFVEEHDSGDGQGGKKSTKTLMFFEGCPKPLCCT 595
           PTVDHVASLKLGYCDAFRVETFVEEHDSGDGQGGKKSTKTLMFFEGCPKPLCCT
Sbjct: 541 PTVDHVASLKLGYCDAFRVETFVEEHDSGDGQGGKKSTKTLMFFEGCPKPLCCT 594

BLAST of MELO3C010117 vs. TrEMBL
Match: A0A0A0M3A5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G662800 PE=4 SV=1)

HSP 1 Score: 981.1 bits (2535), Expect = 1.3e-282
Identity = 492/512 (96.09%), Postives = 501/512 (97.85%), Query Frame = 1

Query: 801  ERLNYVATLRKKHSPDGSSNDISTLEEKELNSLEELVELTLASGRDEFFGGCDNDSLPPE 860
            +RLNYVATLRKKHSPDGSSNDIS L EKELNSLEELVELTLASGRDEFFGGCDNDSLP E
Sbjct: 1127 QRLNYVATLRKKHSPDGSSNDISILVEKELNSLEELVELTLASGRDEFFGGCDNDSLPAE 1186

Query: 861  SKREAGLGREGNSGNISRLNETNREKNIDLGGQEDDFCLSPVNSEPVDVGIGIRRANSVG 920
            SKREAGLGREGNSGNISRLN+T+REKNIDLGGQEDDFCLSPVN +PVDVGIGIRRANSVG
Sbjct: 1187 SKREAGLGREGNSGNISRLNKTDREKNIDLGGQEDDFCLSPVNLDPVDVGIGIRRANSVG 1246

Query: 921  EYPIMTDLSGTLDAVWKGVIHPNSLIFEDSFSRTNSAILEPLAGQSDMEKCTANGIETAH 980
            EYPIMT + GTLDAVWKGVIHPNSLIF+DSFSRT SAILEPLAGQSDMEKCTAN IETAH
Sbjct: 1247 EYPIMT-VPGTLDAVWKGVIHPNSLIFDDSFSRTTSAILEPLAGQSDMEKCTANRIETAH 1306

Query: 981  LLHSALVLTSDDSVETITNSTSMPPSAYNTKISLLNIPKSDISDYSSVFISSYRELEKQS 1040
            LLHSALVLT DDSVETI NSTSMPPSAYNTKISLLNIPKSDISDYSSVFISSYRELEKQS
Sbjct: 1307 LLHSALVLTRDDSVETIPNSTSMPPSAYNTKISLLNIPKSDISDYSSVFISSYRELEKQS 1366

Query: 1041 HLRLLMHVGSSDTVVPVYDEEPTSIIAYSLLMPEYIAQMSEPVKGMEASDTARSLPVIGS 1100
            HLRLLMHVGSSDTVVPVYDEEPTSIIAYS+LMPEYIAQMSEP KGMEASDTARSLPVIGS
Sbjct: 1367 HLRLLMHVGSSDTVVPVYDEEPTSIIAYSMLMPEYIAQMSEPAKGMEASDTARSLPVIGS 1426

Query: 1101 VKMNSIKTCDEEGSDAYKTLRSNEETILSMPRTESLQFEDLVCMTKDLHTRVCFVNETPL 1160
            VKMNSIKTCDEEGSDAYKTLRSNEETILSMPRTESLQFEDLVCM+KDLHTRVCF+NETPL
Sbjct: 1427 VKMNSIKTCDEEGSDAYKTLRSNEETILSMPRTESLQFEDLVCMSKDLHTRVCFINETPL 1486

Query: 1161 GPVKYTITCYFAKRFEALRKKCCPSELDFVRSLSRCRRWGAQGGKSNVFFAKTLDERFII 1220
            GPVKYTITCYFAKRFEALRKKCCPSELDFVRSLSRCRRWGAQGGKSNVFFAKTLDERFII
Sbjct: 1487 GPVKYTITCYFAKRFEALRKKCCPSELDFVRSLSRCRRWGAQGGKSNVFFAKTLDERFII 1546

Query: 1221 KQVTKIELESFIQFSKAYFIYLSQAICTGCPTCLAKVVGMYQVSSKHLKGGKECKVDVLV 1280
            KQVTKIELESFIQFSKAYFIYLSQAICTGCPTCLAKVVGMYQVSSKHLKGGKECKVDVLV
Sbjct: 1547 KQVTKIELESFIQFSKAYFIYLSQAICTGCPTCLAKVVGMYQVSSKHLKGGKECKVDVLV 1606

Query: 1281 MENLLFRHNVTRLYDLKGSSRSRYNPDTSGKN 1313
            MENLLFRHNVTRLYDLKGSSRSRYNPDTSGKN
Sbjct: 1607 MENLLFRHNVTRLYDLKGSSRSRYNPDTSGKN 1637

HSP 2 Score: 438.7 bits (1127), Expect = 2.5e-119
Identity = 204/207 (98.55%), Postives = 204/207 (98.55%), Query Frame = 1

Query: 595  NYRCHSCGMPSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGF 654
            NYRCHSCGMPSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGF
Sbjct: 882  NYRCHSCGMPSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGF 941

Query: 655  PPATRRVIMSDAAWGLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFH 714
            PPATRRVIMSDAAWGLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFH
Sbjct: 942  PPATRRVIMSDAAWGLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFH 1001

Query: 715  YAAINVQSVHIPPSMIEFHLNNLEWINKEANEVHNRAKTLFDEVCKALPLISKNESGAEQ 774
            YAAINVQSVHIPPSMIEFHLNNLEWINKEANEVHNRAKTLF EVCKALPLI KNESGAEQ
Sbjct: 1002 YAAINVQSVHIPPSMIEFHLNNLEWINKEANEVHNRAKTLFGEVCKALPLIPKNESGAEQ 1061

Query: 775  EDFGADELNNYIQELEQIINMEKENFE 802
            EDFG DELNNYIQELEQIINMEKENFE
Sbjct: 1062 EDFGVDELNNYIQELEQIINMEKENFE 1088

HSP 3 Score: 746.9 bits (1927), Expect = 4.2e-212
Identity = 381/621 (61.35%), Postives = 463/621 (74.56%), Query Frame = 1

Query: 1   MGHPENKISDLVGLVKSWMPWRSDPANVSRDFWMPDKSCRVCYECDSQFTFINRRHHCRR 60
           MG+P+NK+SDLV +VKSW+P RS+P NVSRDFWMPD+SCRVCYECDSQFT  NRRHHCR 
Sbjct: 1   MGNPDNKLSDLVDIVKSWIPRRSEPPNVSRDFWMPDQSCRVCYECDSQFTVFNRRHHCRL 60

Query: 61  CGRVFCSKCTANFIPALSTDPTNAREDSEKIRVCNYCFKQWGKEPSSLQDGDKATSSALS 120
           CGRVFC+KCTAN +PA S      +EDSE+IRVCNYCFKQW +  +++  G  A S  LS
Sbjct: 61  CGRVFCAKCTANSVPAPSDVQRAGQEDSERIRVCNYCFKQWEQWIAAVDTGTNAHSPGLS 120

Query: 121 VSSSSTSMGSTKSGYTCHSAISHIASTPCSTSRQYYDPYCSA----------AIADEHDN 180
            S S+TS+ STKS  TC+S+ S + STP ST   +   Y S           A A E +N
Sbjct: 121 PSPSATSLASTKSSCTCNSSSSTVGSTPYSTGPYHRVNYNSGLSPRESSQMNASATEQNN 180

Query: 181 LRNGKTINRTTSLMTSSSSYCGYY--RSDDEDDDYYYGMYRSDSESRHISQSDDYYCAVN 240
             +G + N +++ + SSS++ G    RSDDEDDDY  G Y SDSESRH + ++DYY A+N
Sbjct: 181 KASGTSTNPSSAAVDSSSNHFGLCDNRSDDEDDDY--GAYHSDSESRHYAHAEDYYGAIN 240

Query: 241 I--------------DGGLNLERENTSTKGSPVNFDTQCPRGNEESSEDSDENDKGSECD 300
           I              DGG N++ ++ S    P NF+ Q   G ++  E+ +E +   E +
Sbjct: 241 IGSIDRVYGSDKVHPDGG-NMDTKSLSGSPLPENFNAQSVDGIKKF-EEVNERENADEGE 300

Query: 301 FPTYAMQNKDHEPMDFQNNGILWLPPEPEDEEDEK--ALLFEDDDDVCAPGEWGHLHSSL 360
            P Y +   D EP+DF+NNG+LWLPPEPEDEEDE+  AL  +DDDD  A GEWG+L SS 
Sbjct: 301 VPAYDVDGTDVEPVDFENNGLLWLPPEPEDEEDERESALFDDDDDDEGASGEWGYLRSSN 360

Query: 361 S--SGECPMKGKSSEDYKKTAKNVVEGHFRALVSQLLEAENLPIGDAPNEDGWLDIITYL 420
           S  SGE   + KS+E++++  KNVVEGHFRALV+QLL+ ENLP+GD    D WLDIITYL
Sbjct: 361 SFGSGEYRSRDKSNEEHRRAMKNVVEGHFRALVAQLLQVENLPVGDEDGGDSWLDIITYL 420

Query: 421 SWEAAAVLKPDTSKSGGMDPGGYVKVKCVAGGKRSESIIVKGIICKKNVAHRRMTRGISK 480
           SWEAA +LKPDTSK GGMDPGGYVKVKC+A G+R+ES +VKG++CKKNVAHRRMT  I K
Sbjct: 421 SWEAATLLKPDTSKGGGMDPGGYVKVKCIASGRRNESSVVKGVVCKKNVAHRRMTSKIDK 480

Query: 481 PRFFVLGGALEYQRVTNHLSSFDTLLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQE 540
           PRF +LGGALEYQR+++HLSSFDTLLQQE+DHLKMAVAKIDAHHPNVLLVEKSVSRHAQE
Sbjct: 481 PRFLILGGALEYQRISSHLSSFDTLLQQEMDHLKMAVAKIDAHHPNVLLVEKSVSRHAQE 540

Query: 541 YLLSKNITLVLNIKRSLLERIACCTGANIIPTVDHVASLKLGYCDAFRVETFVEEHDSGD 592
           YLL+K+I+LVLNIKR LLERIA CTGA I+P++DH+ S KLGYCD F VE F+EEH S  
Sbjct: 541 YLLAKDISLVLNIKRPLLERIARCTGAQIVPSIDHLTSPKLGYCDVFHVEKFLEEHGSA- 600

BLAST of MELO3C010117 vs. TrEMBL
Match: A0A061GSN9_THECC (Phosphatidylinositol-4-phosphate 5-kinase family protein, putative isoform 1 OS=Theobroma cacao GN=TCM_037451 PE=4 SV=1)

HSP 1 Score: 701.4 bits (1809), Expect = 2.0e-198
Identity = 392/774 (50.65%), Postives = 520/774 (67.18%), Query Frame = 1

Query: 595  NYRCHSCGMPSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGF 654
            ++RC SC MPSEAHVHCYTH QGSLTIS +KLP+  LPG+ EGKIWMWHRCL+CPR N F
Sbjct: 924  SFRCRSCEMPSEAHVHCYTHRQGSLTISVRKLPELPLPGQREGKIWMWHRCLRCPRANKF 983

Query: 655  PPATRRVIMSDAAWGLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFH 714
            PPATRR++MSDAAWGLSFGKFLELSFSN+AAASRVA CGH LHRDCLRFYGFGR VACF 
Sbjct: 984  PPATRRIVMSDAAWGLSFGKFLELSFSNHAAASRVASCGHSLHRDCLRFYGFGRRVACFR 1043

Query: 715  YAAINVQSVHIPPSMIEFHLNNLEWINKEANEVHNRAKTLFDEVCKALPLISKN------ 774
            YAAI+V SV++PP  +EF+ +N EWI  EANEV NRA+ LF EV  AL  +S+       
Sbjct: 1044 YAAIDVHSVYLPPPKLEFNYDNQEWIQSEANEVTNRAEFLFREVYNALQKMSEKLLGPGF 1103

Query: 775  -ESGAEQ-------EDFGA------DELNNYIQEL--------EQIIN-MEKENFER--- 834
             + G +        E+  A      +E    +QE+        + +I+ +E    +R   
Sbjct: 1104 QDGGIKSPEKRICIEELEAMLQKDREEFQESLQEVLCKEVKVGQPVIDILEINKLQRQIL 1163

Query: 835  -LNYVATLRKKHS--------PDGSSNDISTLEEKELNSLEELVELTLASGRDEFFGGCD 894
             L+YV   R  H+         +  S+ I  L  K ++S+E+LVE+ ++    +    CD
Sbjct: 1164 FLSYVWDQRLIHAFSSIVNNIQEVMSSSIPKLGLKPVSSVEKLVEINVSPKPSKALSSCD 1223

Query: 895  NDSLPPESKREAGLGREGNSGNISRLNETNREKNIDL---GGQEDDFCLS-----PVNSE 954
            +  +  ++K +  + +EGN+G IS     +REK +D       E +  LS        S+
Sbjct: 1224 SALV--QTKPDININQEGNTGEISEPGGDHREKGMDQDLNSRNEAESSLSCSANTSEKSD 1283

Query: 955  PVDVGIGIRRANSVGEYPIMTDLSGTLDAVWKGVIHPNSL-IFEDSFSRTNSAILE-PLA 1014
             ++ G  +RRA S GE+PIM +LS TL+A W G  HP S+   E+ +S +++ +++   A
Sbjct: 1284 SLESGKVVRRALSEGEFPIMANLSDTLEAAWTGESHPASVGPKENGYSVSDTVVVDLSTA 1343

Query: 1015 GQSDMEKCTANG--IETAHLLHSALVLTSDDSVETITNSTSMP-PSAYN--TKISLLNIP 1074
              SDM   T++   +E A    SAL     +++E   +  SMP P+ Y+   K S  N  
Sbjct: 1344 ANSDMGNRTSDRGEVEVACSPQSALPTKGPENMEKTMSWASMPFPNFYSLFNKNSSFNAQ 1403

Query: 1075 KSDISDYSSVFISSYRELEKQSHLRLLMHVGSSDTVVPVYDEEPTSIIAYSLLMPEYIAQ 1134
            K  IS+Y+ V++SS RELE+QS  RLL+ +G +DTVVPVYD+EPTSIIAY+L+  +Y +Q
Sbjct: 1404 KLSISEYNPVYVSSLRELERQSGARLLLPIGVNDTVVPVYDDEPTSIIAYALVSSDYYSQ 1463

Query: 1135 MSEPVKGMEASDTARSLPVIGSVKMNSIKTCDEEGSDAYKTLRSNEETILSMPRTESLQF 1194
            MSE  K  +A+D+A S  +  SV +  + + ++  SD +++  S +E+ILS+  + S   
Sbjct: 1464 MSELEKPKDAADSAVSSSLFDSVNLLLLNSFNDSSSDTFRSFGSGDESILSISGSYSSLV 1523

Query: 1195 EDLVCMTKDLHTRVCFVNETPLGPVKYTITCYFAKRFEALRKKCCPSELDFVRSLSRCRR 1254
             D +  TK+ H RV F ++ PLG VK+++TCY+AK FE+LR+ CCPSELDF+RSLSRC++
Sbjct: 1524 SDPLLDTKNFHARVSFTDDGPLGKVKHSVTCYYAKWFESLRRTCCPSELDFIRSLSRCKK 1583

Query: 1255 WGAQGGKSNVFFAKTLDERFIIKQVTKIELESFIQFSKAYFIYLSQAICTGCPTCLAKVV 1313
            WGAQGGKSNVFFAKTLD+RFIIKQVTK ELESFI+F  AYF YLS +I T  PTCLAK++
Sbjct: 1584 WGAQGGKSNVFFAKTLDDRFIIKQVTKTELESFIKFGPAYFKYLSDSISTRSPTCLAKIL 1643

HSP 2 Score: 746.9 bits (1927), Expect = 4.2e-212
Identity = 381/621 (61.35%), Postives = 463/621 (74.56%), Query Frame = 1

Query: 1   MGHPENKISDLVGLVKSWMPWRSDPANVSRDFWMPDKSCRVCYECDSQFTFINRRHHCRR 60
           MG+P+NK+SDLV +VKSW+P RS+P NVSRDFWMPD+SCRVCYECDSQFT  NRRHHCR 
Sbjct: 1   MGNPDNKLSDLVDIVKSWIPRRSEPPNVSRDFWMPDQSCRVCYECDSQFTVFNRRHHCRL 60

Query: 61  CGRVFCSKCTANFIPALSTDPTNAREDSEKIRVCNYCFKQWGKEPSSLQDGDKATSSALS 120
           CGRVFC+KCTAN +PA S      +EDSE+IRVCNYCFKQW +  +++  G  A S  LS
Sbjct: 61  CGRVFCAKCTANSVPAPSDVQRAGQEDSERIRVCNYCFKQWEQWIAAVDTGTNAHSPGLS 120

Query: 121 VSSSSTSMGSTKSGYTCHSAISHIASTPCSTSRQYYDPYCSA----------AIADEHDN 180
            S S+TS+ STKS  TC+S+ S + STP ST   +   Y S           A A E +N
Sbjct: 121 PSPSATSLASTKSSCTCNSSSSTVGSTPYSTGPYHRVNYNSGLSPRESSQMNASATEQNN 180

Query: 181 LRNGKTINRTTSLMTSSSSYCGYY--RSDDEDDDYYYGMYRSDSESRHISQSDDYYCAVN 240
             +G + N +++ + SSS++ G    RSDDEDDDY  G Y SDSESRH + ++DYY A+N
Sbjct: 181 KASGTSTNPSSAAVDSSSNHFGLCDNRSDDEDDDY--GAYHSDSESRHYAHAEDYYGAIN 240

Query: 241 I--------------DGGLNLERENTSTKGSPVNFDTQCPRGNEESSEDSDENDKGSECD 300
           I              DGG N++ ++ S    P NF+ Q   G ++  E+ +E +   E +
Sbjct: 241 IGSIDRVYGSDKVHPDGG-NMDTKSLSGSPLPENFNAQSVDGIKKF-EEVNERENADEGE 300

Query: 301 FPTYAMQNKDHEPMDFQNNGILWLPPEPEDEEDEK--ALLFEDDDDVCAPGEWGHLHSSL 360
            P Y +   D EP+DF+NNG+LWLPPEPEDEEDE+  AL  +DDDD  A GEWG+L SS 
Sbjct: 301 VPAYDVDGTDVEPVDFENNGLLWLPPEPEDEEDERESALFDDDDDDEGASGEWGYLRSSN 360

Query: 361 S--SGECPMKGKSSEDYKKTAKNVVEGHFRALVSQLLEAENLPIGDAPNEDGWLDIITYL 420
           S  SGE   + KS+E++++  KNVVEGHFRALV+QLL+ ENLP+GD    D WLDIITYL
Sbjct: 361 SFGSGEYRSRDKSNEEHRRAMKNVVEGHFRALVAQLLQVENLPVGDEDGGDSWLDIITYL 420

Query: 421 SWEAAAVLKPDTSKSGGMDPGGYVKVKCVAGGKRSESIIVKGIICKKNVAHRRMTRGISK 480
           SWEAA +LKPDTSK GGMDPGGYVKVKC+A G+R+ES +VKG++CKKNVAHRRMT  I K
Sbjct: 421 SWEAATLLKPDTSKGGGMDPGGYVKVKCIASGRRNESSVVKGVVCKKNVAHRRMTSKIDK 480

Query: 481 PRFFVLGGALEYQRVTNHLSSFDTLLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQE 540
           PRF +LGGALEYQR+++HLSSFDTLLQQE+DHLKMAVAKIDAHHPNVLLVEKSVSRHAQE
Sbjct: 481 PRFLILGGALEYQRISSHLSSFDTLLQQEMDHLKMAVAKIDAHHPNVLLVEKSVSRHAQE 540

Query: 541 YLLSKNITLVLNIKRSLLERIACCTGANIIPTVDHVASLKLGYCDAFRVETFVEEHDSGD 592
           YLL+K+I+LVLNIKR LLERIA CTGA I+P++DH+ S KLGYCD F VE F+EEH S  
Sbjct: 541 YLLAKDISLVLNIKRPLLERIARCTGAQIVPSIDHLTSPKLGYCDVFHVEKFLEEHGSA- 600

BLAST of MELO3C010117 vs. TrEMBL
Match: A0A061GJU0_THECC (Phosphatidylinositol-4-phosphate 5-kinase family protein, putative isoform 2 (Fragment) OS=Theobroma cacao GN=TCM_037451 PE=4 SV=1)

HSP 1 Score: 701.4 bits (1809), Expect = 2.0e-198
Identity = 392/774 (50.65%), Postives = 520/774 (67.18%), Query Frame = 1

Query: 595  NYRCHSCGMPSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGF 654
            ++RC SC MPSEAHVHCYTH QGSLTIS +KLP+  LPG+ EGKIWMWHRCL+CPR N F
Sbjct: 924  SFRCRSCEMPSEAHVHCYTHRQGSLTISVRKLPELPLPGQREGKIWMWHRCLRCPRANKF 983

Query: 655  PPATRRVIMSDAAWGLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFH 714
            PPATRR++MSDAAWGLSFGKFLELSFSN+AAASRVA CGH LHRDCLRFYGFGR VACF 
Sbjct: 984  PPATRRIVMSDAAWGLSFGKFLELSFSNHAAASRVASCGHSLHRDCLRFYGFGRRVACFR 1043

Query: 715  YAAINVQSVHIPPSMIEFHLNNLEWINKEANEVHNRAKTLFDEVCKALPLISKN------ 774
            YAAI+V SV++PP  +EF+ +N EWI  EANEV NRA+ LF EV  AL  +S+       
Sbjct: 1044 YAAIDVHSVYLPPPKLEFNYDNQEWIQSEANEVTNRAEFLFREVYNALQKMSEKLLGPGF 1103

Query: 775  -ESGAEQ-------EDFGA------DELNNYIQEL--------EQIIN-MEKENFER--- 834
             + G +        E+  A      +E    +QE+        + +I+ +E    +R   
Sbjct: 1104 QDGGIKSPEKRICIEELEAMLQKDREEFQESLQEVLCKEVKVGQPVIDILEINKLQRQIL 1163

Query: 835  -LNYVATLRKKHS--------PDGSSNDISTLEEKELNSLEELVELTLASGRDEFFGGCD 894
             L+YV   R  H+         +  S+ I  L  K ++S+E+LVE+ ++    +    CD
Sbjct: 1164 FLSYVWDQRLIHAFSSIVNNIQEVMSSSIPKLGLKPVSSVEKLVEINVSPKPSKALSSCD 1223

Query: 895  NDSLPPESKREAGLGREGNSGNISRLNETNREKNIDL---GGQEDDFCLS-----PVNSE 954
            +  +  ++K +  + +EGN+G IS     +REK +D       E +  LS        S+
Sbjct: 1224 SALV--QTKPDININQEGNTGEISEPGGDHREKGMDQDLNSRNEAESSLSCSANTSEKSD 1283

Query: 955  PVDVGIGIRRANSVGEYPIMTDLSGTLDAVWKGVIHPNSL-IFEDSFSRTNSAILE-PLA 1014
             ++ G  +RRA S GE+PIM +LS TL+A W G  HP S+   E+ +S +++ +++   A
Sbjct: 1284 SLESGKVVRRALSEGEFPIMANLSDTLEAAWTGESHPASVGPKENGYSVSDTVVVDLSTA 1343

Query: 1015 GQSDMEKCTANG--IETAHLLHSALVLTSDDSVETITNSTSMP-PSAYN--TKISLLNIP 1074
              SDM   T++   +E A    SAL     +++E   +  SMP P+ Y+   K S  N  
Sbjct: 1344 ANSDMGNRTSDRGEVEVACSPQSALPTKGPENMEKTMSWASMPFPNFYSLFNKNSSFNAQ 1403

Query: 1075 KSDISDYSSVFISSYRELEKQSHLRLLMHVGSSDTVVPVYDEEPTSIIAYSLLMPEYIAQ 1134
            K  IS+Y+ V++SS RELE+QS  RLL+ +G +DTVVPVYD+EPTSIIAY+L+  +Y +Q
Sbjct: 1404 KLSISEYNPVYVSSLRELERQSGARLLLPIGVNDTVVPVYDDEPTSIIAYALVSSDYYSQ 1463

Query: 1135 MSEPVKGMEASDTARSLPVIGSVKMNSIKTCDEEGSDAYKTLRSNEETILSMPRTESLQF 1194
            MSE  K  +A+D+A S  +  SV +  + + ++  SD +++  S +E+ILS+  + S   
Sbjct: 1464 MSELEKPKDAADSAVSSSLFDSVNLLLLNSFNDSSSDTFRSFGSGDESILSISGSYSSLV 1523

Query: 1195 EDLVCMTKDLHTRVCFVNETPLGPVKYTITCYFAKRFEALRKKCCPSELDFVRSLSRCRR 1254
             D +  TK+ H RV F ++ PLG VK+++TCY+AK FE+LR+ CCPSELDF+RSLSRC++
Sbjct: 1524 SDPLLDTKNFHARVSFTDDGPLGKVKHSVTCYYAKWFESLRRTCCPSELDFIRSLSRCKK 1583

Query: 1255 WGAQGGKSNVFFAKTLDERFIIKQVTKIELESFIQFSKAYFIYLSQAICTGCPTCLAKVV 1313
            WGAQGGKSNVFFAKTLD+RFIIKQVTK ELESFI+F  AYF YLS +I T  PTCLAK++
Sbjct: 1584 WGAQGGKSNVFFAKTLDDRFIIKQVTKTELESFIKFGPAYFKYLSDSISTRSPTCLAKIL 1643

HSP 2 Score: 746.9 bits (1927), Expect = 4.2e-212
Identity = 381/621 (61.35%), Postives = 463/621 (74.56%), Query Frame = 1

Query: 1   MGHPENKISDLVGLVKSWMPWRSDPANVSRDFWMPDKSCRVCYECDSQFTFINRRHHCRR 60
           MG+P+NK+SDLV +VKSW+P RS+P NVSRDFWMPD+SCRVCYECDSQFT  NRRHHCR 
Sbjct: 1   MGNPDNKLSDLVDIVKSWIPRRSEPPNVSRDFWMPDQSCRVCYECDSQFTVFNRRHHCRL 60

Query: 61  CGRVFCSKCTANFIPALSTDPTNAREDSEKIRVCNYCFKQWGKEPSSLQDGDKATSSALS 120
           CGRVFC+KCTAN +PA S      +EDSE+IRVCNYCFKQW +  +++  G  A S  LS
Sbjct: 61  CGRVFCAKCTANSVPAPSDVQRAGQEDSERIRVCNYCFKQWEQWIAAVDTGTNAHSPGLS 120

Query: 121 VSSSSTSMGSTKSGYTCHSAISHIASTPCSTSRQYYDPYCSA----------AIADEHDN 180
            S S+TS+ STKS  TC+S+ S + STP ST   +   Y S           A A E +N
Sbjct: 121 PSPSATSLASTKSSCTCNSSSSTVGSTPYSTGPYHRVNYNSGLSPRESSQMNASATEQNN 180

Query: 181 LRNGKTINRTTSLMTSSSSYCGYY--RSDDEDDDYYYGMYRSDSESRHISQSDDYYCAVN 240
             +G + N +++ + SSS++ G    RSDDEDDDY  G Y SDSESRH + ++DYY A+N
Sbjct: 181 KASGTSTNPSSAAVDSSSNHFGLCDNRSDDEDDDY--GAYHSDSESRHYAHAEDYYGAIN 240

Query: 241 I--------------DGGLNLERENTSTKGSPVNFDTQCPRGNEESSEDSDENDKGSECD 300
           I              DGG N++ ++ S    P NF+ Q   G ++  E+ +E +   E +
Sbjct: 241 IGSIDRVYGSDKVHPDGG-NMDTKSLSGSPLPENFNAQSVDGIKKF-EEVNERENADEGE 300

Query: 301 FPTYAMQNKDHEPMDFQNNGILWLPPEPEDEEDEK--ALLFEDDDDVCAPGEWGHLHSSL 360
            P Y +   D EP+DF+NNG+LWLPPEPEDEEDE+  AL  +DDDD  A GEWG+L SS 
Sbjct: 301 VPAYDVDGTDVEPVDFENNGLLWLPPEPEDEEDERESALFDDDDDDEGASGEWGYLRSSN 360

Query: 361 S--SGECPMKGKSSEDYKKTAKNVVEGHFRALVSQLLEAENLPIGDAPNEDGWLDIITYL 420
           S  SGE   + KS+E++++  KNVVEGHFRALV+QLL+ ENLP+GD    D WLDIITYL
Sbjct: 361 SFGSGEYRSRDKSNEEHRRAMKNVVEGHFRALVAQLLQVENLPVGDEDGGDSWLDIITYL 420

Query: 421 SWEAAAVLKPDTSKSGGMDPGGYVKVKCVAGGKRSESIIVKGIICKKNVAHRRMTRGISK 480
           SWEAA +LKPDTSK GGMDPGGYVKVKC+A G+R+ES +VKG++CKKNVAHRRMT  I K
Sbjct: 421 SWEAATLLKPDTSKGGGMDPGGYVKVKCIASGRRNESSVVKGVVCKKNVAHRRMTSKIDK 480

Query: 481 PRFFVLGGALEYQRVTNHLSSFDTLLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQE 540
           PRF +LGGALEYQR+++HLSSFDTLLQQE+DHLKMAVAKIDAHHPNVLLVEKSVSRHAQE
Sbjct: 481 PRFLILGGALEYQRISSHLSSFDTLLQQEMDHLKMAVAKIDAHHPNVLLVEKSVSRHAQE 540

Query: 541 YLLSKNITLVLNIKRSLLERIACCTGANIIPTVDHVASLKLGYCDAFRVETFVEEHDSGD 592
           YLL+K+I+LVLNIKR LLERIA CTGA I+P++DH+ S KLGYCD F VE F+EEH S  
Sbjct: 541 YLLAKDISLVLNIKRPLLERIARCTGAQIVPSIDHLTSPKLGYCDVFHVEKFLEEHGSA- 600

BLAST of MELO3C010117 vs. TrEMBL
Match: A0A061GL64_THECC (Phosphatidylinositol-4-phosphate 5-kinase family protein, putative isoform 3 OS=Theobroma cacao GN=TCM_037451 PE=4 SV=1)

HSP 1 Score: 701.4 bits (1809), Expect = 2.0e-198
Identity = 392/774 (50.65%), Postives = 520/774 (67.18%), Query Frame = 1

Query: 595  NYRCHSCGMPSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGF 654
            ++RC SC MPSEAHVHCYTH QGSLTIS +KLP+  LPG+ EGKIWMWHRCL+CPR N F
Sbjct: 924  SFRCRSCEMPSEAHVHCYTHRQGSLTISVRKLPELPLPGQREGKIWMWHRCLRCPRANKF 983

Query: 655  PPATRRVIMSDAAWGLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFH 714
            PPATRR++MSDAAWGLSFGKFLELSFSN+AAASRVA CGH LHRDCLRFYGFGR VACF 
Sbjct: 984  PPATRRIVMSDAAWGLSFGKFLELSFSNHAAASRVASCGHSLHRDCLRFYGFGRRVACFR 1043

Query: 715  YAAINVQSVHIPPSMIEFHLNNLEWINKEANEVHNRAKTLFDEVCKALPLISKN------ 774
            YAAI+V SV++PP  +EF+ +N EWI  EANEV NRA+ LF EV  AL  +S+       
Sbjct: 1044 YAAIDVHSVYLPPPKLEFNYDNQEWIQSEANEVTNRAEFLFREVYNALQKMSEKLLGPGF 1103

Query: 775  -ESGAEQ-------EDFGA------DELNNYIQEL--------EQIIN-MEKENFER--- 834
             + G +        E+  A      +E    +QE+        + +I+ +E    +R   
Sbjct: 1104 QDGGIKSPEKRICIEELEAMLQKDREEFQESLQEVLCKEVKVGQPVIDILEINKLQRQIL 1163

Query: 835  -LNYVATLRKKHS--------PDGSSNDISTLEEKELNSLEELVELTLASGRDEFFGGCD 894
             L+YV   R  H+         +  S+ I  L  K ++S+E+LVE+ ++    +    CD
Sbjct: 1164 FLSYVWDQRLIHAFSSIVNNIQEVMSSSIPKLGLKPVSSVEKLVEINVSPKPSKALSSCD 1223

Query: 895  NDSLPPESKREAGLGREGNSGNISRLNETNREKNIDL---GGQEDDFCLS-----PVNSE 954
            +  +  ++K +  + +EGN+G IS     +REK +D       E +  LS        S+
Sbjct: 1224 SALV--QTKPDININQEGNTGEISEPGGDHREKGMDQDLNSRNEAESSLSCSANTSEKSD 1283

Query: 955  PVDVGIGIRRANSVGEYPIMTDLSGTLDAVWKGVIHPNSL-IFEDSFSRTNSAILE-PLA 1014
             ++ G  +RRA S GE+PIM +LS TL+A W G  HP S+   E+ +S +++ +++   A
Sbjct: 1284 SLESGKVVRRALSEGEFPIMANLSDTLEAAWTGESHPASVGPKENGYSVSDTVVVDLSTA 1343

Query: 1015 GQSDMEKCTANG--IETAHLLHSALVLTSDDSVETITNSTSMP-PSAYN--TKISLLNIP 1074
              SDM   T++   +E A    SAL     +++E   +  SMP P+ Y+   K S  N  
Sbjct: 1344 ANSDMGNRTSDRGEVEVACSPQSALPTKGPENMEKTMSWASMPFPNFYSLFNKNSSFNAQ 1403

Query: 1075 KSDISDYSSVFISSYRELEKQSHLRLLMHVGSSDTVVPVYDEEPTSIIAYSLLMPEYIAQ 1134
            K  IS+Y+ V++SS RELE+QS  RLL+ +G +DTVVPVYD+EPTSIIAY+L+  +Y +Q
Sbjct: 1404 KLSISEYNPVYVSSLRELERQSGARLLLPIGVNDTVVPVYDDEPTSIIAYALVSSDYYSQ 1463

Query: 1135 MSEPVKGMEASDTARSLPVIGSVKMNSIKTCDEEGSDAYKTLRSNEETILSMPRTESLQF 1194
            MSE  K  +A+D+A S  +  SV +  + + ++  SD +++  S +E+ILS+  + S   
Sbjct: 1464 MSELEKPKDAADSAVSSSLFDSVNLLLLNSFNDSSSDTFRSFGSGDESILSISGSYSSLV 1523

Query: 1195 EDLVCMTKDLHTRVCFVNETPLGPVKYTITCYFAKRFEALRKKCCPSELDFVRSLSRCRR 1254
             D +  TK+ H RV F ++ PLG VK+++TCY+AK FE+LR+ CCPSELDF+RSLSRC++
Sbjct: 1524 SDPLLDTKNFHARVSFTDDGPLGKVKHSVTCYYAKWFESLRRTCCPSELDFIRSLSRCKK 1583

Query: 1255 WGAQGGKSNVFFAKTLDERFIIKQVTKIELESFIQFSKAYFIYLSQAICTGCPTCLAKVV 1313
            WGAQGGKSNVFFAKTLD+RFIIKQVTK ELESFI+F  AYF YLS +I T  PTCLAK++
Sbjct: 1584 WGAQGGKSNVFFAKTLDDRFIIKQVTKTELESFIKFGPAYFKYLSDSISTRSPTCLAKIL 1643

HSP 2 Score: 735.7 bits (1898), Expect = 9.7e-209
Identity = 375/623 (60.19%), Postives = 464/623 (74.48%), Query Frame = 1

Query: 1   MGHPENKISDLVGLVKSWMPWRSDPANVSRDFWMPDKSCRVCYECDSQFTFINRRHHCRR 60
           MG P+NK+S+LV + KSW+P RS+P NVSRDFWMPD+SCRVCY+CDSQFT  NRRHHCR 
Sbjct: 1   MGTPDNKLSELVDIFKSWIPRRSEPPNVSRDFWMPDQSCRVCYDCDSQFTIFNRRHHCRL 60

Query: 61  CGRVFCSKCTANFIPALSTDPTNAREDSEKIRVCNYCFKQWGKEPSSLQDGDKATSSALS 120
           CGRVFC+KCTAN +PA S +    RED E+IRVCNYCF+QW +  +++ +G  A S  LS
Sbjct: 61  CGRVFCAKCTANSVPAPSDEQRAGREDWERIRVCNYCFRQWEQGIATVDNGPPARSPGLS 120

Query: 121 VSSSSTSMGSTKSGYTCHSAISHIASTPCSTSRQYYDPYCSA-----------AIADEHD 180
            S S+TS+ STKS  TCHS+ S I STP ST      PY S            ++    D
Sbjct: 121 PSPSATSLASTKSSCTCHSSSSTIGSTPYSTGPYQRVPYTSGLSPNQSSPQIDSVTATQD 180

Query: 181 NLRNGKTINRTTSLMTSSSSYCGY--YRSDDEDDDYYYGMYRSDSESRHISQSDDYYCAV 240
           N  + ++I+   ++  SS ++ G+   RSDDEDDDY  G+YR DSE  H S ++DYY AV
Sbjct: 181 NCTSQRSISSDAAMAESSPNHFGFGMNRSDDEDDDY--GVYRLDSEPSHFSHANDYYGAV 240

Query: 241 NIDG--------GLNLERENTSTKGSPVNFDTQCPRGNEESSEDSDENDKGSECDFPTYA 300
           NI+          ++L+ +NTS+   P  FDTQ   G++E  E+S E++   EC+   Y 
Sbjct: 241 NIEEFDNVYGPHNVHLDGDNTSSL-LPEGFDTQGVEGSQELREESYEHNNCDECETSPYD 300

Query: 301 MQNKDHEPMDFQNNGILWLPPEPEDEEDEK-ALLFEDDDDVC-----APGEWGHLHSSLS 360
           +Q+ + EP+DF+NNG+LWLPPEPEDEEDE+ A+LF++DDD       A GEWG+L SS S
Sbjct: 301 LQSTNAEPVDFENNGLLWLPPEPEDEEDEREAVLFDEDDDDGGGVGGATGEWGYLRSSNS 360

Query: 361 --SGECPMKGKSSEDYKKTAKNVVEGHFRALVSQLLEAENLPIGDAPNEDGWLDIITYLS 420
             +GEC  + KS E+++   KNVVEGHFRALV+QLL+ E+LP+GD  N++ WLDIIT LS
Sbjct: 361 FGNGECRTREKSIEEHRNAMKNVVEGHFRALVAQLLQVESLPLGDEDNKESWLDIITSLS 420

Query: 421 WEAAAVLKPDTSKSGGMDPGGYVKVKCVAGGKRSESIIVKGIICKKNVAHRRMTRGISKP 480
           WEAA +LKPDTSK GGMDPGGYVKVKC+A G+R+ES +VKG++CKKNVAHRRMT  I KP
Sbjct: 421 WEAATLLKPDTSKGGGMDPGGYVKVKCIACGRRNESTVVKGVVCKKNVAHRRMTSKIEKP 480

Query: 481 RFFVLGGALEYQRVTNHLSSFDTLLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEY 540
           RF +LGGALEYQRV+N LSSFDTLLQQE+DHLKMAVAKID+HHPNVLLVEKSVSR+AQ+Y
Sbjct: 481 RFLILGGALEYQRVSNLLSSFDTLLQQEMDHLKMAVAKIDSHHPNVLLVEKSVSRYAQDY 540

Query: 541 LLSKNITLVLNIKRSLLERIACCTGANIIPTVDHVASLKLGYCDAFRVETFVEEHDSGDG 595
           LL+K+I+LVLNIKR LLERIA CTGA I+P++DH+ S KLGYCD F VE F E H S  G
Sbjct: 541 LLAKDISLVLNIKRPLLERIARCTGAQIVPSIDHLISPKLGYCDIFHVEKFFEVHGSA-G 600

BLAST of MELO3C010117 vs. TAIR10
Match: AT3G14270.1 (AT3G14270.1 phosphatidylinositol-4-phosphate 5-kinase family protein)

HSP 1 Score: 601.7 bits (1550), Expect = 1.1e-171
Identity = 358/765 (46.80%), Postives = 465/765 (60.78%), Query Frame = 1

Query: 595  NYRCHSCGMPSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGF 654
            +YRC SC MPSEAHVHCYTH QGSLTIS KKL D+ LPGE EGKIWMWHRCL+CPR NGF
Sbjct: 905  SYRCRSCEMPSEAHVHCYTHRQGSLTISVKKLQDYLLPGEKEGKIWMWHRCLRCPRLNGF 964

Query: 655  PPATRRVIMSDAAWGLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFH 714
            PPAT RV+MSDAAWGLSFGKFLELSFSN+AAASRVA CGH LHRDCLRFYGFG  VACF 
Sbjct: 965  PPATLRVVMSDAAWGLSFGKFLELSFSNHAAASRVACCGHSLHRDCLRFYGFGNMVACFR 1024

Query: 715  YAAINVQSVHIPPSMIEFHLNNLEWINKEANEVHNRAKTLFDEVCKALPLISKNESGAEQ 774
            YA I+V SV++PPS++ F+  N +WI +E +EV  RA+ LF EV  A+  I+  E G  +
Sbjct: 1025 YATIDVHSVYLPPSILSFNYENQDWIQRETDEVIERAELLFSEVLNAISQIA--EKGFRR 1084

Query: 775  EDFGADELNNYIQELEQIINMEKENFERLNYVATLRKKHSPDGSSNDISTLEEKELNSLE 834
                       I ELE+++  EK  FE  N    L ++ +      DI  L       L 
Sbjct: 1085 R----------IGELEEVLQKEKAEFEE-NMQKILHREVNEGQPLVDILELYRIHRQLLF 1144

Query: 835  ELV----ELTLASGRDEFFGGCD----NDSLPPESK-----------------REAGLGR 894
            +       L  AS   +     D     +  PP +K                  E  L  
Sbjct: 1145 QSYMWDHRLINASTLHKLENSDDTKREENEKPPLAKSQTLPEMNAGTNSLLTGSEVNLNP 1204

Query: 895  EGNS-GNISRLNETNRE--KNIDL------GGQEDDFCLSPVNSEPVDVGIGIRRANSVG 954
            +G+S G+   LN   +E   N DL      GG+       P  S P++  + +RR  S G
Sbjct: 1205 DGDSTGDTGSLNNVQKEADTNSDLYQEKDDGGEVSPSKTLPDTSYPLENKVDVRRTQSDG 1264

Query: 955  EYPIMTDLSGTLDAVWKG------VIHPNSLIFEDSFSRTNSAILEPLAG---QSDMEKC 1014
            +  +M +LS TLDA W G       I  N+ +     + +NS+   P++      D+ + 
Sbjct: 1265 QI-VMKNLSATLDAAWIGERQTSVEIPTNNKVSLPPSTMSNSSTFPPISEGLMPIDLPE- 1324

Query: 1015 TANGIETAHLLHSALVLTSDDSVETITNSTSMPPSAYNTKIS---LLNIPKSD-ISDYSS 1074
              N  + A+ +  AL   + ++ E   +  S+P   +   I+   LL+  K D   ++S 
Sbjct: 1325 QQNEFKVAYPVSPALPSKNYENSEDSVSWLSVPFLNFYRSINKNFLLSSQKLDTFGEHSP 1384

Query: 1075 VFISSYRELEKQSHLRLLMHVGSSDTVVPVYDEEPTSIIAYSLLMPEYIAQMSEPVKGME 1134
            ++ISS+RE E Q   RLL+ VG +D VVPVYD+EPTS+IAY+L+ PEY  Q S   + + 
Sbjct: 1385 IYISSFREAELQGGPRLLLPVGLNDIVVPVYDDEPTSMIAYALMSPEYQRQTSAEGESLV 1444

Query: 1135 ASDTARSLPVIGSVKMNSIKTCDEEGSDAYKTLRSNEETILSMPRTESLQFEDLVCMTKD 1194
            +  +  ++P          +  D+   D  ++  S +E+ILS+  + S    D +  TK 
Sbjct: 1445 SYPSELNIP----------RPVDDTIFDPSRSNGSVDESILSISSSRSTSLLDPLSYTKA 1504

Query: 1195 LHTRVCFVNETPLGPVKYTITCYFAKRFEALRKKCCPSELDFVRSLSRCRRWGAQGGKSN 1254
            LH RV +  +  LG VKYT+TCY+AKRFEALR  C PSEL+++RSLSRC++WGAQGGKSN
Sbjct: 1505 LHARVSYGEDGTLGKVKYTVTCYYAKRFEALRGICLPSELEYIRSLSRCKKWGAQGGKSN 1564

Query: 1255 VFFAKTLDERFIIKQVTKIELESFIQFSKAYFIYLSQAICTGCPTCLAKVVGMYQVSSKH 1313
            VFFAKTLD+RFIIKQVTK ELESFI+F+ AYF YLS++I T  PTCLAK++G+YQV++K 
Sbjct: 1565 VFFAKTLDDRFIIKQVTKTELESFIKFAPAYFKYLSESISTKSPTCLAKILGIYQVATKQ 1624

HSP 2 Score: 598.2 bits (1541), Expect = 1.2e-170
Identity = 326/615 (53.01%), Postives = 416/615 (67.64%), Query Frame = 1

Query: 9   SDLVGLVKSWMPWRSDPANVSRDFWMPDKSCRVCYECDSQFTFINRRHHCRRCGRVFCSK 68
           S++VGL+KSW+PWRS+PA VSRDFWMPD+SCRVCYECD QFT INRRHHCR CGRVFC K
Sbjct: 12  SEIVGLIKSWLPWRSEPATVSRDFWMPDQSCRVCYECDCQFTLINRRHHCRHCGRVFCGK 71

Query: 69  CTANFIPALSTDPTNAREDSEKIRVCNYCFKQWGKEPSSLQDGDKATSSA--LSVSSSST 128
           CTAN IP   +D    RED E+IRVCNYCF+QW +      DG    S+   LS S S T
Sbjct: 72  CTANSIPFAPSDLRTPREDWERIRVCNYCFRQWEQG-----DGGPHVSNITELSTSPSET 131

Query: 129 SMGSTKSGYTCHSAISHIASTPCS---TSRQYY--DPYCSAAIADEHDNLRNGKTINRTT 188
           S+ S+K+  T +S+   + S P       R ++  D       + E    + GK  +R +
Sbjct: 132 SLLSSKTSTTANSSSFALGSMPGLIGLNQRVHHGSDVSLHGVSSMETSVTKQGKETSRRS 191

Query: 189 SLMTS-----SSSYCGYYRSDDEDDDYYYGMYRSDSESRHISQSDDYYCAVNIDG-GLN- 248
           S + +     S       RSDDE D+Y  G Y++D E+ H  ++++YY  +  +G G++ 
Sbjct: 192 SFIATDVEDPSRFALNSIRSDDEYDEY--GAYQTDIETSHSPRANEYYGPMEYNGMGIDD 251

Query: 249 --------LERENTSTKGSPVNFDTQCP----RGNEESSEDSDENDKGSECDFPTYAMQN 308
                      +  S  GSP+    QC     R   E  +   E+D   EC+  + A  +
Sbjct: 252 VPCKHLGGETADQKSLSGSPLIH--QCLESLIREGSEQFQKKSEHDGRDECEASSPADIS 311

Query: 309 KDH--EPMDFQNNGILWLPPEPEDEEDEK--ALLFEDDDDVCAPGEWGHLHSSLS--SGE 368
            D   EP+DF+NNG+LW+PPEPE+EEDE+  AL  E+D++  A GEWG+L  S S  SGE
Sbjct: 312 DDQVVEPVDFENNGLLWVPPEPENEEDERESALFDEEDNEGDASGEWGYLRPSTSFGSGE 371

Query: 369 CPMKGKSSEDYKKTAKNVVEGHFRALVSQLLEAENLPIGDAPNEDGWLDIITYLSWEAAA 428
              + +++E++KK  KNVV+GHFRAL++QLL+ EN+ + D   ++ WL+IIT LSWEAA 
Sbjct: 372 YRGEDRTTEEHKKAMKNVVDGHFRALLAQLLQVENISVSDEEGKESWLEIITSLSWEAAN 431

Query: 429 VLKPDTSKSGGMDPGGYVKVKCVAGGKRSESIIVKGIICKKNVAHRRMTRGISKPRFFVL 488
           +LKPD SKSGGMDPGGYVKVKC+A G R +S++VKG++CKKNV +RRM+  I K R  +L
Sbjct: 432 LLKPDMSKSGGMDPGGYVKVKCLASGFRHDSMVVKGVVCKKNVVNRRMSTKIEKARLLIL 491

Query: 489 GGALEYQRVTNHLSSFDTLLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKN 548
           GG LEYQRV+N LSSFDTLLQQE DHLKMAVAKI A  PN+LLVEKSVSR AQEYLL+K+
Sbjct: 492 GGGLEYQRVSNQLSSFDTLLQQEKDHLKMAVAKIHAERPNILLVEKSVSRFAQEYLLAKD 551

Query: 549 ITLVLNIKRSLLERIACCTGANIIPTVDHVASLKLGYCDAFRVETFVEEHDSGDGQGGKK 592
           I+LVLNIKR LL+RIA CTGA IIP+VDH++S KLGYC+ FRV+ + EEH S  GQ GKK
Sbjct: 552 ISLVLNIKRPLLDRIARCTGAQIIPSVDHLSSQKLGYCENFRVDRYPEEHGS-TGQVGKK 611

BLAST of MELO3C010117 vs. TAIR10
Match: AT4G33240.1 (AT4G33240.1 1-phosphatidylinositol-4-phosphate 5-kinases;zinc ion binding;1-phosphatidylinositol-3-phosphate 5-kinases)

HSP 1 Score: 579.3 bits (1492), Expect = 5.9e-165
Identity = 337/759 (44.40%), Postives = 455/759 (59.95%), Query Frame = 1

Query: 598  CHSCGMPSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPA 657
            C SC MP+EAH+HCYTH QGSLTIS KKLP+  LPG+ EGKIWMWHRCLKCPR NGFPPA
Sbjct: 873  CPSCTMPAEAHIHCYTHRQGSLTISVKKLPEL-LPGQREGKIWMWHRCLKCPRINGFPPA 932

Query: 658  TRRVIMSDAAWGLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAA 717
            TRR++MSDAAWGLSFGKFLELSFSN+AAASRVA CGH LHRDCLRFYGFGR VACF YA+
Sbjct: 933  TRRIVMSDAAWGLSFGKFLELSFSNHAAASRVANCGHSLHRDCLRFYGFGRMVACFRYAS 992

Query: 718  INVQSVHIPPSMIEFHLNNLEWINKEANEVHNRAKTLFDEVCKALPLISKNESGAEQEDF 777
            IN+ +V +PP+ + F+  N EW+ KE+ EV  +A+ LF+EV +AL  IS    GA  +  
Sbjct: 993  INIYAVTLPPAKLYFNYENQEWLQKESKEVIKKAEVLFNEVQEALSQISAKTMGAGSKGS 1052

Query: 778  GADELNNYIQELEQIINMEKENF-ERLNYVATLRKKHSPDGSSNDISTLEEKELNSLEEL 837
              +++   ++EL  ++   K+ + + L  +  + K   P        T++   +N L  L
Sbjct: 1053 TPNKIKLSLEELAGLLEQRKKEYKDSLQQMLNVVKDGQP--------TIDILLINKLRRL 1112

Query: 838  VELTLASGRDEFFGGC-----DNDSLPPESKREAGLGREGNSGNISRLNETNREKNIDLG 897
            + +  +   DE   G      +N    P++     +GR  +   +S     +   ++ + 
Sbjct: 1113 I-IFDSYAWDECLAGAANMVRNNYLEAPKNSAPKVMGRNVSLEKLSDEKVKSIPTHVAIC 1172

Query: 898  GQEDDFCLSPVNSEPVDVGIGIRRANSVGEYPIMTDLSGTLDAVWKGVIHPNSLIFEDSF 957
               +D  L   + E   +  G   A++ G++ I  D+        +    P S   +D+F
Sbjct: 1173 ---NDSLLQDADYETC-LNQGKSFADTSGKFAIPEDVGSDRPPDCRMEFDP-SEGGKDNF 1232

Query: 958  SRTNSAILEPLAGQSDMEKCTANGIETAHLLHSALVLTSDDSV------ETITNSTSMPP 1017
               +S +++P   +S  +    +  +T          TS++ +         TN T +P 
Sbjct: 1233 VE-SSQVVKPAHTESQFQATDLS--DTLDAAWIGEQTTSENGIFRPPSRAASTNGTQIPD 1292

Query: 1018 ------------------SAYNTKISL--------------LNIPKSDISDYSSVFISSY 1077
                                + T++ L              LN  K  +++   V++SSY
Sbjct: 1293 LRLLGSESELNFKGGPTNDEHTTQVQLPSPSFYYSLNKNYSLNSRKHIMAEDRPVYVSSY 1352

Query: 1078 RELEKQSHLRLLMHVGSSDTVVPVYDEEPTSIIAYSLLMPEYIAQMSEPVKGMEASDTAR 1137
            RELE +S  RLL+ +G +D V+PVYD+EPTSIIAY+L   EY AQMS   K  +  D+  
Sbjct: 1353 RELEWRSGARLLLPLGCNDLVLPVYDDEPTSIIAYALTSSEYKAQMSGSDKSRDRLDSGG 1412

Query: 1138 SLPVIGSVKMNSIKTCDEEGSDAYKTLRSNEETILSMPRTESLQFEDLVCMTKDLHTRVC 1197
            S  +  SV + S+ +  +   D  ++L S +E +  +  +            KDLH R+ 
Sbjct: 1413 SFSLFDSVNLLSLNSLSDLSVDMSRSLSSADEQVSQLLHSS--------LYLKDLHARIS 1472

Query: 1198 FVNETPLGPVKYTITCYFAKRFEALRKKCCPSELDFVRSLSRCRRWGAQGGKSNVFFAKT 1257
            F +E P G VKY++TCY+AK FEALR  CCPSE DF+RSL RCR+WGAQGGKSNVFFAK+
Sbjct: 1473 FTDEGPPGKVKYSVTCYYAKEFEALRMICCPSETDFIRSLGRCRKWGAQGGKSNVFFAKS 1532

Query: 1258 LDERFIIKQVTKIELESFIQFSKAYFIYLSQAICTGCPTCLAKVVGMYQVSSKHLKGGKE 1313
            LD+RFIIKQVTK ELESFI+F  AYF YL+++I T  PT LAK++G+YQVSSKHLKGGKE
Sbjct: 1533 LDDRFIIKQVTKTELESFIKFGPAYFKYLTESISTKSPTSLAKILGIYQVSSKHLKGGKE 1592

HSP 2 Score: 575.9 bits (1483), Expect = 6.5e-164
Identity = 316/624 (50.64%), Postives = 408/624 (65.38%), Query Frame = 1

Query: 1   MGHPENKISDLVGLVKSWMPWRSDPANVSRDFWMPDKSCRVCYECDSQFTFINRRHHCRR 60
           M   ++K    V +VKSW+P +S+ +N+SRDFWMPD+SC VCYECD+QFT  NRRHHCR 
Sbjct: 1   MDSQDHKAPGFVDIVKSWIPRKSESSNMSRDFWMPDQSCPVCYECDAQFTVFNRRHHCRL 60

Query: 61  CGRVFCSKCTANFIPALSTDPTNAREDSEKIRVCNYCFKQWGKEPSSLQDGDKATSSALS 120
           CGRVFC+KC AN IP+ S +  ++ E+ E+IRVCNYC+KQW +      +G    S   S
Sbjct: 61  CGRVFCAKCAANSIPSPSDETKDSHEEPERIRVCNYCYKQWEQGIVPPDNGASIISLHFS 120

Query: 121 VSSSSTSMGSTKSGYTCHSAISHIASTPCSTSRQYYDPYCSAAIAD-------EHDNLRN 180
            S S+ S+ ST    T +S+   I ST   + R   +P  S  ++        E  N ++
Sbjct: 121 SSPSARSVAST----TSNSSNCTIDSTAGPSPRPKMNPRASRRVSSNMDSEKSEQQNAKS 180

Query: 181 GKTINRTTSLMTSSSSYCGYY-----RSD---DEDDDYYYGMYRSDSESRHISQSDDYYC 240
            ++ +    ++ SS +   ++     RSD   D+DDDY     +S       +Q +DYY 
Sbjct: 181 RRSSDHYGHVLDSSDNQVEFFVNSSGRSDGEADDDDDYQSDFAQS------YAQGNDYYG 240

Query: 241 AVNIDG-------------GLNLERENTSTKGSPVNFDTQCPRGNEESSEDSDENDKGSE 300
           A+N+D              G+ +E    +  G P + D               EN     
Sbjct: 241 AINLDEVDHIYGSHEAHDVGVKIE---PNISGFPPDQDLDSLNTETIDKTRQQENGWNDV 300

Query: 301 CDFPTYAMQNKDHEPMDFQNNGILWLPPEPEDEEDEK-ALLFEDDDDVCAPGEWGHLHSS 360
            +      ++ + E +DF+++G+LWLPPEPE+EEDE+ A+L +DD D    G+WG+L  S
Sbjct: 301 KEGSPPCEESFEPEVVDFESDGLLWLPPEPENEEDEREAVLSDDDGDEGDRGDWGYLRPS 360

Query: 361 LSSGECPMKGKSSEDYKKTA-KNVVEGHFRALVSQLLEAENLPIGDAPNEDGWLDIITYL 420
            S  E   K   S+D    A KNVVEGHFRALV+QLLE +NLP+ +  +E+GWLDIIT L
Sbjct: 361 NSFNE---KDFHSKDKSSGAMKNVVEGHFRALVAQLLEVDNLPMVNEGDEEGWLDIITSL 420

Query: 421 SWEAAAVLKPDTSKSGGMDPGGYVKVKCVAGGKRSESIIVKGIICKKNVAHRRMTRGISK 480
           SWEAA +LKPDTSKSGGMDPGGYVKVKC+  G+RSES++VKG++CKKNVAHRRMT  I K
Sbjct: 421 SWEAATLLKPDTSKSGGMDPGGYVKVKCIPCGRRSESMVVKGVVCKKNVAHRRMTSKIEK 480

Query: 481 PRFFVLGGALEYQRVTNHLSSFDTLLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQE 540
           PR  +LGGALEYQR++N LSSFDTLLQQE+DHLKMAVAKID+H+P++LLVEKSVSR AQE
Sbjct: 481 PRLLILGGALEYQRISNQLSSFDTLLQQEMDHLKMAVAKIDSHNPDILLVEKSVSRFAQE 540

Query: 541 YLLSKNITLVLNIKRSLLERIACCTGANIIPTVDHVASLKLGYCDAFRVETFVEEHDSGD 595
           YLL+K+I+LVLNIKRSLLERI+ CTGA I+P++D + S KLGYCD F VE FVE H S  
Sbjct: 541 YLLAKDISLVLNIKRSLLERISRCTGAQIVPSIDQLTSPKLGYCDLFHVEKFVETHVS-P 600

BLAST of MELO3C010117 vs. TAIR10
Match: AT1G71010.1 (AT1G71010.1 FORMS APLOID AND BINUCLEATE CELLS 1C)

HSP 1 Score: 451.4 bits (1160), Expect = 1.8e-126
Identity = 285/744 (38.31%), Postives = 411/744 (55.24%), Query Frame = 1

Query: 598  CHSCGMPSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPA 657
            C SC    +AHV CY+H  G+LTI+ ++LP   LPGE +GKIWMWHRCL+C   +G PPA
Sbjct: 825  CRSCKELVDAHVLCYSHQNGNLTINVRRLPSMKLPGEQDGKIWMWHRCLRCAHVDGVPPA 884

Query: 658  TRRVIMSDAAWGLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAA 717
            TRRV+MSDAAWGLSFGKFLELSFSN+A A+RVA CGH L RDCLRFYGFG  VA F Y+ 
Sbjct: 885  TRRVVMSDAAWGLSFGKFLELSFSNHATANRVASCGHSLQRDCLRFYGFGNMVAFFRYSP 944

Query: 718  INVQSVHIPPSMIEFHLN-NLEWINKEANEVHNRAKTLFDEVCKALPLISKNESGAEQED 777
            IN+ +V +PPSM+EF+ +   EWI  EA E+  + +T++ E+   L  + +  S  E E 
Sbjct: 945  INILTVLLPPSMLEFNSHPQQEWIRTEAAELVGKMRTMYTEISDMLNRMEEKSSLLEPEQ 1004

Query: 778  FGADELNNYIQELEQIINMEKENFERLNYVATLRKKHSPDGSSNDISTLEEKELNSLEEL 837
              A +L++ I  L   +  EK+ ++  + +  + +++     S DI      ELN L   
Sbjct: 1005 SEACDLHSRIIGLIDQLVKEKDEYD--DALQPIFEENLQIQGSLDIL-----ELNRLRRA 1064

Query: 838  V---------ELTLASGR----DEFFGGCDNDSLPPESKREAGLGREGNSGNISR--LNE 897
            +         +L L + +      F  G DN    PE      + R    G+  R   + 
Sbjct: 1065 LMIGAHAWDHQLYLLNSQLKKASVFKTGDDNAPRNPEMHDPPKIDRRMQEGSDERDEQSH 1124

Query: 898  TNREKNIDLGGQEDDFCLSPVNSEPVDVGIGIRRANSVGEYPIMTDLSGTLDAVWKGVIH 957
            T+ E N D             N +P ++        S G     T LS  +D+ W G   
Sbjct: 1125 TDSEANGD-------------NKDPENIP-------SPG-----TSLSERIDSAWLGSFQ 1184

Query: 958  ----PNSLIFEDSFSRTNSAI---LEPLAGQS-----DMEKCTANGIETAHLLHSAL--V 1017
                  ++   + FS  NS++     P+  QS       ++    G+  + L  S L   
Sbjct: 1185 NLEKAETIAETEGFSAVNSSLRRLARPIRVQSFDSAIRFQERIQKGLPPSSLYLSTLRSF 1244

Query: 1018 LTSDDSVETITNSTSMPPSAYNTKISLLNIPKSDISDYSS-VFISSYRELEKQSHLRLLM 1077
              S +    + +  S     Y+  + L  + K D+   S+  +ISS  ++   + + L+ 
Sbjct: 1245 HASGEYRNMVRDPVSNVMRTYSQMLPL-EVQKLDLIVGSAPTYISSASQMADGARM-LIP 1304

Query: 1078 HVGSSDTVVPVYDEEPTSIIAYSLLMPEYIAQMSEPVKGMEASDTARSLPVIGSVKMNSI 1137
              G +D VVPVYD++P S+++Y++   EY   +    KG+ +S ++ +L         + 
Sbjct: 1305 QRGLNDIVVPVYDDDPASVVSYAINSKEYKEWIVN--KGLASSSSSSNL---------NN 1364

Query: 1138 KTCDEEGSDAYKTLRSNEETILSMPRTESLQFEDLVCMTKDLHTRVCFVNETPLGPVKYT 1197
            +  +      +++L  + + I       S        +T     R    +    G VK++
Sbjct: 1365 RESEPSAFSTWRSLSMDVDYIQHAVYGSSQDDRKSPHLTISFSDRASSSSTATEGKVKFS 1424

Query: 1198 ITCYFAKRFEALRKKCCPSELDFVRSLSRCRRWGAQGGKSNVFFAKTLDERFIIKQVTKI 1257
            +TCYFA +F+ LRK CCPSE+DFVRSLSRC+RW AQGGKSNV+FAK+LDERFIIKQV K 
Sbjct: 1425 VTCYFATQFDTLRKTCCPSEVDFVRSLSRCQRWSAQGGKSNVYFAKSLDERFIIKQVVKT 1484

Query: 1258 ELESFIQFSKAYFIYLSQAICTGCPTCLAKVVGMYQVSSKHLKGGKECKVDVLVMENLLF 1311
            EL+SF  F+  YF YL +++ +G PTCLAK++G+YQVS KH KGGKE K+D++VMENL +
Sbjct: 1485 ELDSFEDFAPEYFKYLKESLSSGSPTCLAKILGIYQVSIKHPKGGKETKMDLMVMENLFY 1523

HSP 2 Score: 325.9 bits (834), Expect = 1.2e-88
Identity = 183/433 (42.26%), Postives = 253/433 (58.43%), Query Frame = 1

Query: 190 YCGYYRSDDEDDDYYYGMYRSDSESRHISQSDDYYCAVNIDGGLNLE---RENTSTKGSP 249
           YCG        D+Y      SD ES  +S   + +   +  G    +   R N S  G  
Sbjct: 148 YCGKQLLSPSSDNYQDS---SDIESGSVSARHELFSCKSSAGSSPHDSPLRNNFSPLGRF 207

Query: 250 VNF------DTQCPRGNEESSEDSDENDKGSECDFPTYAMQNKDHE--------PMDFQN 309
           V         T C   N +    +D   K  +       ++ +DHE        P+DF+N
Sbjct: 208 VQHAKDLRSPTVCSFDNHQEQLLADNLVKPGQG-----VLEQEDHEEEEDKLQQPLDFEN 267

Query: 310 NGILWLPPEPEDEEDEKALLF----EDDDDVCAPGEWGHLHSSLSSGECPMKGKSSEDYK 369
           NG +W PP PEDE D+    +    ++DDD+        L SS SS   P K K  E+  
Sbjct: 268 NGRIWYPPPPEDENDDAESNYFHYDDEDDDIGDSATEFSLSSSFSS-HIPTKEKLGENSN 327

Query: 370 KTAKNVVEGHFRALVSQLLEAENLPIGDAPNEDGWLDIITYLSWEAAAVLKPDTSKSGGM 429
           +  + VV  HFRALV++LL  E L   D  +   WLDI+T L+W+AA  +KPDT   G M
Sbjct: 328 EPLRTVVHDHFRALVAELLRGEELSPSDDGSAGEWLDIVTALAWQAANFVKPDTRAGGSM 387

Query: 430 DPGGYVKVKCVAGGKRSESIIVKGIICKKNVAHRRMTRGISKPRFFVLGGALEYQRVTNH 489
           DPG YVK+KCVA G ++ESI+++GI+C KN+ H+RM      PR  +L G+LEYQRV   
Sbjct: 388 DPGNYVKIKCVASGNQNESILIRGIVCSKNITHKRMISQYKNPRVMLLAGSLEYQRVAGQ 447

Query: 490 LSSFDTLLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLL 549
           L+SF+TLLQQE +H+K  +AKI++  PNVLLVEKS S +AQ+YLL K I+LVLN+KRSLL
Sbjct: 448 LASFNTLLQQENEHMKAIIAKIESLRPNVLLVEKSASSYAQQYLLEKEISLVLNVKRSLL 507

Query: 550 ERIACCTGANIIPTVDHVASLKLGYCDAFRVETFVEEHDSGDGQGGKKSTKTLMFFEGCP 602
           +RIA CTGA + P++D +++ +LG+C+ FR E  +E+H++G+ Q  +K ++TLM+FEGCP
Sbjct: 508 DRIARCTGAVLCPSLDSISTARLGHCELFRTERVLEQHEAGN-QSNRKPSRTLMYFEGCP 567

BLAST of MELO3C010117 vs. TAIR10
Match: AT1G34260.1 (AT1G34260.1 FORMS APLOID AND BINUCLEATE CELLS 1A)

HSP 1 Score: 201.8 bits (512), Expect = 2.6e-51
Identity = 144/398 (36.18%), Postives = 208/398 (52.26%), Query Frame = 1

Query: 211 DSESRHISQSDDYYCAVNIDGGLNLERENTSTKGSPVNFDTQCPRGNEESSEDSDENDKG 270
           D  S H SQ D     V +   + ++R    +K  P +        +E+S E+S EN   
Sbjct: 31  DECSSHSSQED-----VELTKEVKVDRLERKSKSMPSDILDIL---DEKSKENSVEN--- 90

Query: 271 SECDFPTYAMQNKDHEPMDFQNNGILWLPPEPEDEEDEKALLFEDDDDVCAPGEWGHLHS 330
               F +    + D  P+        W PPEPE+ EDE   +F DDDD C  G   +  S
Sbjct: 91  --VQFLSDREDDSDDVPV--------WEPPEPENPEDEVDGVFADDDDDCCDGSKWNKAS 150

Query: 331 SLS--SGECPMKGKSSEDYKKTAKNVVEGHFRALVSQLLEAENLPIGDAPNEDG-WLDII 390
            L   S E   K K  E+ ++      +  F+ +VSQL+++    I     E G W +I+
Sbjct: 151 LLGELSDESSEKRKVYEENRRVMLEEADSKFKFIVSQLIKSAGFSI----EESGYWFEIV 210

Query: 391 TYLSWEAAAVLKPDTSKSGGMDPGGYVKVKCVAGGKRSESIIVKGIICKKNVAHRRMTRG 450
             L WEAA++LKP       +DP  Y+KVKC+A G   +S + KG++ KK+ A + M   
Sbjct: 211 ARLCWEAASMLKPAIDGKS-VDPTEYIKVKCIATGSCVDSEVFKGLVFKKHAALKHMATK 270

Query: 451 ISKPRFFVLGGALEYQRVTNHLSSFDTLLQQE---LDHLKMAVAKIDAHHPNVLLVEKSV 510
              PR  ++ G L +    +  SS  ++ Q     L ++K  V  I+A  P+V+LVEKSV
Sbjct: 271 YEHPRIMLVEGVLGHP--ISGFSSLQSVNQDNEYLLKYVKPVVDIIEASKPDVMLVEKSV 330

Query: 511 SRHAQEYLLSKNITLVLNIKRSLLERIACCTGANIIPTVDHVASLKLGYCDAFRVETFVE 570
           SR  Q+ +L K +TLV ++K   L+RI+ C G+ I+ +VD ++S KL +CD+FR+E  VE
Sbjct: 331 SRDIQKTILDKGVTLVFDMKLHRLQRISRCIGSPIL-SVDSLSSQKLKHCDSFRIEKIVE 390

Query: 571 EHDSGDGQGGKKSTKTLMFFEGCPKPLCCTNY--RCHS 601
           EH++  G+  KK TKTLMF EGCP  L CT     CHS
Sbjct: 391 EHNAA-GESDKKPTKTLMFLEGCPTRLGCTILLKGCHS 398

HSP 2 Score: 163.3 bits (412), Expect = 1.0e-39
Identity = 102/284 (35.92%), Postives = 161/284 (56.69%), Query Frame = 1

Query: 1022 ISDYSSVFISSYRELEKQSHLRLLMHVGSSDTVVPVYDEEPTSIIAYSLLMPEYIAQMSE 1081
            +++++   + +  ++  +   RL + +   D +V  Y++E +S+IA +L      A ++ 
Sbjct: 1063 VNNFTQENLQTVNQIITEESSRLRISLRDDDFIVSDYEDELSSLIACAL------AHLNN 1122

Query: 1082 PVKGMEASDTARSLPVIGSVKMNSIKTCDEEGSDAYKTLRSNEETILSMPRTESLQFEDL 1141
                 E S    S  + GS++       D + +D   +  S+E T     R E+L   ++
Sbjct: 1123 -----EESKKPLSRCIHGSLQGFLDNNQDSKQTDRDVSRFSSEST----NRLETLPPPEV 1182

Query: 1142 VCMTKDLHTRVCFVNETPLGPVKYTITCYFAKRFEALRKKCCPSELDFVRSLSRCRRWGA 1201
            +         V F +   +G  KY+I   +A  F  LRK+CC SELD++ SLSRC+ W A
Sbjct: 1183 L---------VTFGSVKSVGKPKYSIVSLYADDFRDLRKRCCSSELDYIASLSRCKPWDA 1242

Query: 1202 QGGKSNVFFAKTLDERFIIKQVTKIELESFIQFSKAYFIYLSQAICTGCPTCLAKVVGMY 1261
            +GGKS   FAKTLD+RFI+K++ K E ESF+ F+  YF Y+  +   G  TCLAKV+G++
Sbjct: 1243 KGGKSKSVFAKTLDDRFIVKEIKKTEYESFVTFATEYFKYMKDSYDLGNQTCLAKVLGIH 1302

Query: 1262 QVSSKHLK-GGKECKVDVLVMENLLFRHNVTRLYDLKGSSRSRY 1305
            QV+ +  K GGKE + D++VMENL F   VTR YDLKG+  +R+
Sbjct: 1303 QVTVRQPKGGGKEIRHDLMVMENLSFSRKVTRQYDLKGALHARF 1322

HSP 3 Score: 129.8 bits (325), Expect = 1.2e-29
Identity = 74/206 (35.92%), Postives = 103/206 (50.00%), Query Frame = 1

Query: 598 CHSCGMPSEAHVHCYTHPQGSLTISAKKLPDFF-LPGEPEGKIWMWHRCLKCPRTNGFPP 657
           C +C    EAH++ Y H    LTI  K++P    L GE +GKIWMW RC KC   N    
Sbjct: 647 CQTCVEFPEAHLYYYAHQNKQLTIQIKRIPVAKGLAGEAKGKIWMWSRCGKCKTKNASRK 706

Query: 658 ATRRVIMSDAAWGLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYA 717
           +T+RV++S AA  LSFGKFLELSFS     +R + CGH    D L F+G G  VA   Y+
Sbjct: 707 STKRVLISTAARSLSFGKFLELSFSQQTFLNRSSSCGHSFDSDFLHFFGLGSMVAMLSYS 766

Query: 718 AINVQSVHIPPSMIEFH-LNNLEWINKEANEVHNRAKTLFDEVCKALPLISKNESGAEQE 777
            +   +V +PP  +E   L    W+ KE   V  +  +LF++   A   + +  S     
Sbjct: 767 QVASYTVSLPPMKLESSILIKAGWLEKEFQTVFTKGISLFED---AAGFLKRLRSQFTNS 826

Query: 778 DFGADELNNYIQELEQIINMEKENFE 802
           D         +  +E+++  E+  FE
Sbjct: 827 DLRYQRARKLLSNIEELLKHERCIFE 849

BLAST of MELO3C010117 vs. TAIR10
Match: AT1G20110.1 (AT1G20110.1 RING/FYVE/PHD zinc finger superfamily protein)

HSP 1 Score: 70.5 bits (171), Expect = 8.9e-12
Identity = 31/86 (36.05%), Postives = 44/86 (51.16%), Query Frame = 1

Query: 25  PANVSRDFWMPDKSCRVCYECDSQFTFINRRHHCRRCGRVFCSKCTANFIPALSTDPTNA 84
           P N  +D W+PD++   C  C S F    RRHHCR CG VFC KCT   I         A
Sbjct: 444 PVNEEKDHWVPDEAVSKCTSCGSDFGAFIRRHHCRNCGDVFCDKCTQGRI------ALTA 503

Query: 85  REDSEKIRVCNYCFKQWGKEPSSLQD 111
            +++ ++RVC+ C  +  +  S+ ++
Sbjct: 504 EDNAPQVRVCDRCMAEVSQRLSNAKE 523

BLAST of MELO3C010117 vs. NCBI nr
Match: gi|659086075|ref|XP_008443752.1| (PREDICTED: LOW QUALITY PROTEIN: 1-phosphatidylinositol-3-phosphate 5-kinase FAB1B-like [Cucumis melo])

HSP 1 Score: 1242.6 bits (3214), Expect = 0.0e+00
Identity = 594/594 (100.00%), Postives = 594/594 (100.00%), Query Frame = 1

Query: 1   MGHPENKISDLVGLVKSWMPWRSDPANVSRDFWMPDKSCRVCYECDSQFTFINRRHHCRR 60
           MGHPENKISDLVGLVKSWMPWRSDPANVSRDFWMPDKSCRVCYECDSQFTFINRRHHCRR
Sbjct: 1   MGHPENKISDLVGLVKSWMPWRSDPANVSRDFWMPDKSCRVCYECDSQFTFINRRHHCRR 60

Query: 61  CGRVFCSKCTANFIPALSTDPTNAREDSEKIRVCNYCFKQWGKEPSSLQDGDKATSSALS 120
           CGRVFCSKCTANFIPALSTDPTNAREDSEKIRVCNYCFKQWGKEPSSLQDGDKATSSALS
Sbjct: 61  CGRVFCSKCTANFIPALSTDPTNAREDSEKIRVCNYCFKQWGKEPSSLQDGDKATSSALS 120

Query: 121 VSSSSTSMGSTKSGYTCHSAISHIASTPCSTSRQYYDPYCSAAIADEHDNLRNGKTINRT 180
           VSSSSTSMGSTKSGYTCHSAISHIASTPCSTSRQYYDPYCSAAIADEHDNLRNGKTINRT
Sbjct: 121 VSSSSTSMGSTKSGYTCHSAISHIASTPCSTSRQYYDPYCSAAIADEHDNLRNGKTINRT 180

Query: 181 TSLMTSSSSYCGYYRSDDEDDDYYYGMYRSDSESRHISQSDDYYCAVNIDGGLNLERENT 240
           TSLMTSSSSYCGYYRSDDEDDDYYYGMYRSDSESRHISQSDDYYCAVNIDGGLNLERENT
Sbjct: 181 TSLMTSSSSYCGYYRSDDEDDDYYYGMYRSDSESRHISQSDDYYCAVNIDGGLNLERENT 240

Query: 241 STKGSPVNFDTQCPRGNEESSEDSDENDKGSECDFPTYAMQNKDHEPMDFQNNGILWLPP 300
           STKGSPVNFDTQCPRGNEESSEDSDENDKGSECDFPTYAMQNKDHEPMDFQNNGILWLPP
Sbjct: 241 STKGSPVNFDTQCPRGNEESSEDSDENDKGSECDFPTYAMQNKDHEPMDFQNNGILWLPP 300

Query: 301 EPEDEEDEKALLFEDDDDVCAPGEWGHLHSSLSSGECPMKGKSSEDYKKTAKNVVEGHFR 360
           EPEDEEDEKALLFEDDDDVCAPGEWGHLHSSLSSGECPMKGKSSEDYKKTAKNVVEGHFR
Sbjct: 301 EPEDEEDEKALLFEDDDDVCAPGEWGHLHSSLSSGECPMKGKSSEDYKKTAKNVVEGHFR 360

Query: 361 ALVSQLLEAENLPIGDAPNEDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYVKVKCVA 420
           ALVSQLLEAENLPIGDAPNEDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYVKVKCVA
Sbjct: 361 ALVSQLLEAENLPIGDAPNEDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYVKVKCVA 420

Query: 421 GGKRSESIIVKGIICKKNVAHRRMTRGISKPRFFVLGGALEYQRVTNHLSSFDTLLQQEL 480
           GGKRSESIIVKGIICKKNVAHRRMTRGISKPRFFVLGGALEYQRVTNHLSSFDTLLQQEL
Sbjct: 421 GGKRSESIIVKGIICKKNVAHRRMTRGISKPRFFVLGGALEYQRVTNHLSSFDTLLQQEL 480

Query: 481 DHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCTGANII 540
           DHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCTGANII
Sbjct: 481 DHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCTGANII 540

Query: 541 PTVDHVASLKLGYCDAFRVETFVEEHDSGDGQGGKKSTKTLMFFEGCPKPLCCT 595
           PTVDHVASLKLGYCDAFRVETFVEEHDSGDGQGGKKSTKTLMFFEGCPKPLCCT
Sbjct: 541 PTVDHVASLKLGYCDAFRVETFVEEHDSGDGQGGKKSTKTLMFFEGCPKPLCCT 594

BLAST of MELO3C010117 vs. NCBI nr
Match: gi|659086075|ref|XP_008443752.1| (PREDICTED: LOW QUALITY PROTEIN: 1-phosphatidylinositol-3-phosphate 5-kinase FAB1B-like [Cucumis melo])

HSP 1 Score: 1018.8 bits (2633), Expect = 8.2e-294
Identity = 510/512 (99.61%), Postives = 511/512 (99.80%), Query Frame = 1

Query: 801  ERLNYVATLRKKHSPDGSSNDISTLEEKELNSLEELVELTLASGRDEFFGGCDNDSLPPE 860
            +RLNYVATLRKKHSPDGSSNDISTLEEKELNSLEELVELTLASGRDEFFGGCDNDSLPPE
Sbjct: 1127 QRLNYVATLRKKHSPDGSSNDISTLEEKELNSLEELVELTLASGRDEFFGGCDNDSLPPE 1186

Query: 861  SKREAGLGREGNSGNISRLNETNREKNIDLGGQEDDFCLSPVNSEPVDVGIGIRRANSVG 920
            SKREAGLGREGNSGNISRLNETNREKNIDLGGQEDDFCLSPVNSEPVDVGIGIRRANSVG
Sbjct: 1187 SKREAGLGREGNSGNISRLNETNREKNIDLGGQEDDFCLSPVNSEPVDVGIGIRRANSVG 1246

Query: 921  EYPIMTDLSGTLDAVWKGVIHPNSLIFEDSFSRTNSAILEPLAGQSDMEKCTANGIETAH 980
            EYPIMTDLSGTLDAVWKGVIHPNSLIFEDSFSRTNSAILEPLAGQSDMEKCTANGIETAH
Sbjct: 1247 EYPIMTDLSGTLDAVWKGVIHPNSLIFEDSFSRTNSAILEPLAGQSDMEKCTANGIETAH 1306

Query: 981  LLHSALVLTSDDSVETITNSTSMPPSAYNTKISLLNIPKSDISDYSSVFISSYRELEKQS 1040
            LLHSALVLTSDDSVETITNSTSMPPSAYNTKISLLNIPKSDISDYSSVFISSYRELEKQS
Sbjct: 1307 LLHSALVLTSDDSVETITNSTSMPPSAYNTKISLLNIPKSDISDYSSVFISSYRELEKQS 1366

Query: 1041 HLRLLMHVGSSDTVVPVYDEEPTSIIAYSLLMPEYIAQMSEPVKGMEASDTARSLPVIGS 1100
            HLRLLMHVGSSDTVVPVYDEEPTSIIAYSLLMPEYIAQMSEPVKGMEASDTARSLPVIGS
Sbjct: 1367 HLRLLMHVGSSDTVVPVYDEEPTSIIAYSLLMPEYIAQMSEPVKGMEASDTARSLPVIGS 1426

Query: 1101 VKMNSIKTCDEEGSDAYKTLRSNEETILSMPRTESLQFEDLVCMTKDLHTRVCFVNETPL 1160
            VKMNSIKTCDEEGSDAYKTLRSNEETILSMPRTESLQFEDLVCMTKDLHTRVCFVNETPL
Sbjct: 1427 VKMNSIKTCDEEGSDAYKTLRSNEETILSMPRTESLQFEDLVCMTKDLHTRVCFVNETPL 1486

Query: 1161 GPVKYTITCYFAKRFEALRKKCCPSELDFVRSLSRCRRWGAQGGKSNVFFAKTLDERFII 1220
            GPVKYTITCYFAKRFEALRKKCCPSELDFVRSLSRCRRWGAQGGKSNVFFAKTLDERFII
Sbjct: 1487 GPVKYTITCYFAKRFEALRKKCCPSELDFVRSLSRCRRWGAQGGKSNVFFAKTLDERFII 1546

Query: 1221 KQVTKIELESFIQFSKAYFIYLSQAICTGCPTCLAKVVGMYQVSSKHLKGGKECKVDVLV 1280
            KQVTKIELESFIQFSKAYFIYLSQAICTGCPTCLAKVVGMYQVSSKHLKGGKECKVDVLV
Sbjct: 1547 KQVTKIELESFIQFSKAYFIYLSQAICTGCPTCLAKVVGMYQVSSKHLKGGKECKVDVLV 1606

Query: 1281 MENLLFRHNVTRLYDLKGSSRSRYNPDTSGKN 1313
            MENLLFRHNVTRLYDLKGSSRSRYNPDTSG N
Sbjct: 1607 MENLLFRHNVTRLYDLKGSSRSRYNPDTSGXN 1638

HSP 2 Score: 444.9 bits (1143), Expect = 4.9e-121
Identity = 207/207 (100.00%), Postives = 207/207 (100.00%), Query Frame = 1

Query: 595  NYRCHSCGMPSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGF 654
            NYRCHSCGMPSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGF
Sbjct: 882  NYRCHSCGMPSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGF 941

Query: 655  PPATRRVIMSDAAWGLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFH 714
            PPATRRVIMSDAAWGLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFH
Sbjct: 942  PPATRRVIMSDAAWGLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFH 1001

Query: 715  YAAINVQSVHIPPSMIEFHLNNLEWINKEANEVHNRAKTLFDEVCKALPLISKNESGAEQ 774
            YAAINVQSVHIPPSMIEFHLNNLEWINKEANEVHNRAKTLFDEVCKALPLISKNESGAEQ
Sbjct: 1002 YAAINVQSVHIPPSMIEFHLNNLEWINKEANEVHNRAKTLFDEVCKALPLISKNESGAEQ 1061

Query: 775  EDFGADELNNYIQELEQIINMEKENFE 802
            EDFGADELNNYIQELEQIINMEKENFE
Sbjct: 1062 EDFGADELNNYIQELEQIINMEKENFE 1088

HSP 3 Score: 1208.4 bits (3125), Expect = 0.0e+00
Identity = 579/594 (97.47%), Postives = 585/594 (98.48%), Query Frame = 1

Query: 1   MGHPENKISDLVGLVKSWMPWRSDPANVSRDFWMPDKSCRVCYECDSQFTFINRRHHCRR 60
           MGHPENKISDLVGLVKSWMPWRSDP NVSRDFWMPDKSCRVCYECDSQFTFINRRHHCRR
Sbjct: 1   MGHPENKISDLVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYECDSQFTFINRRHHCRR 60

Query: 61  CGRVFCSKCTANFIPALSTDPTNAREDSEKIRVCNYCFKQWGKEPSSLQDGDKATSSALS 120
           CGRVFCS+CTANFIPA STD TNAREDSEKIRVCNYCFKQWGKEPSSLQDGDKATSSALS
Sbjct: 61  CGRVFCSRCTANFIPAPSTDATNAREDSEKIRVCNYCFKQWGKEPSSLQDGDKATSSALS 120

Query: 121 VSSSSTSMGSTKSGYTCHSAISHIASTPCSTSRQYYDPYCSAAIADEHDNLRNGKTINRT 180
           +SSSSTSMGSTKSGYTCHSAISHIASTPCST RQYYDPYCSAA+ADEHDNLRNGKTINRT
Sbjct: 121 LSSSSTSMGSTKSGYTCHSAISHIASTPCSTGRQYYDPYCSAAMADEHDNLRNGKTINRT 180

Query: 181 TSLMTSSSSYCGYYRSDDEDDDYYYGMYRSDSESRHISQSDDYYCAVNIDGGLNLERENT 240
           TSLMTSSSSY GYYRSDDEDDDYYYGMYRSDSESRHISQSDDYYCAVNID G NLERENT
Sbjct: 181 TSLMTSSSSYYGYYRSDDEDDDYYYGMYRSDSESRHISQSDDYYCAVNIDEGSNLERENT 240

Query: 241 STKGSPVNFDTQCPRGNEESSEDSDENDKGSECDFPTYAMQNKDHEPMDFQNNGILWLPP 300
           STKGSPVNFDTQ PRGNEESSEDSDENDKGSECDFP+YAMQNK+HEPMDFQNNGILWLPP
Sbjct: 241 STKGSPVNFDTQSPRGNEESSEDSDENDKGSECDFPSYAMQNKNHEPMDFQNNGILWLPP 300

Query: 301 EPEDEEDEKALLFEDDDDVCAPGEWGHLHSSLSSGECPMKGKSSEDYKKTAKNVVEGHFR 360
           EPEDEEDEKALLFEDDDDVCAPGEWG+LHSSLSSGECPMKGKSSEDYKKTAKNVVEGHFR
Sbjct: 301 EPEDEEDEKALLFEDDDDVCAPGEWGYLHSSLSSGECPMKGKSSEDYKKTAKNVVEGHFR 360

Query: 361 ALVSQLLEAENLPIGDAPNEDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYVKVKCVA 420
           ALVSQLLEAENLPIGDAP EDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYVKVKCVA
Sbjct: 361 ALVSQLLEAENLPIGDAPREDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYVKVKCVA 420

Query: 421 GGKRSESIIVKGIICKKNVAHRRMTRGISKPRFFVLGGALEYQRVTNHLSSFDTLLQQEL 480
           GGKRSESIIVKGIICKKNVAHRRMTRGISKPRFFVLGGALEYQRVTNHLSSFDTLLQQEL
Sbjct: 421 GGKRSESIIVKGIICKKNVAHRRMTRGISKPRFFVLGGALEYQRVTNHLSSFDTLLQQEL 480

Query: 481 DHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCTGANII 540
           DHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCTGANII
Sbjct: 481 DHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCTGANII 540

Query: 541 PTVDHVASLKLGYCDAFRVETFVEEHDSGDGQGGKKSTKTLMFFEGCPKPLCCT 595
           PTVDHVASLKLGYCDAFRVETFVEEHDSGDGQGGKKSTKTLMFFEGCPKPLCCT
Sbjct: 541 PTVDHVASLKLGYCDAFRVETFVEEHDSGDGQGGKKSTKTLMFFEGCPKPLCCT 594

BLAST of MELO3C010117 vs. NCBI nr
Match: gi|778664202|ref|XP_011660242.1| (PREDICTED: 1-phosphatidylinositol-3-phosphate 5-kinase FAB1B-like isoform X2 [Cucumis sativus])

HSP 1 Score: 439.1 bits (1128), Expect = 2.7e-119
Identity = 209/236 (88.56%), Postives = 212/236 (89.83%), Query Frame = 1

Query: 595  NYRCHSCGMPSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGF 654
            NYRCHSCGMPSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGF
Sbjct: 882  NYRCHSCGMPSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGF 941

Query: 655  PPATRRVIMSDAAWGLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFH 714
            PPATRRVIMSDAAWGLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFH
Sbjct: 942  PPATRRVIMSDAAWGLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFH 1001

Query: 715  YAAINVQSVHIPPSMIEFHLNNLEWINKEANEVHNRAKTLFDEVCKALPLISKNESGAEQ 774
            YAAINVQSVHIPPSMIEFHLNNLEWINKEANEVHNRAKTLF EVCKALPLI KNESGAEQ
Sbjct: 1002 YAAINVQSVHIPPSMIEFHLNNLEWINKEANEVHNRAKTLFGEVCKALPLIPKNESGAEQ 1061

Query: 775  EDFGADELNNYIQELEQIINMEKENFERLNYVATLRKKHSPDGSSNDISTLEEKEL 831
            EDFG DELNNYIQELEQIINMEKENFE       +  KH   G    +  L  + L
Sbjct: 1062 EDFGVDELNNYIQELEQIINMEKENFE-------VSSKHLKGGKECKVDVLVMENL 1110

HSP 2 Score: 107.1 bits (266), Expect = 2.4e-19
Identity = 50/52 (96.15%), Postives = 52/52 (100.00%), Query Frame = 1

Query: 1261 YQVSSKHLKGGKECKVDVLVMENLLFRHNVTRLYDLKGSSRSRYNPDTSGKN 1313
            ++VSSKHLKGGKECKVDVLVMENLLFRHNVTRLYDLKGSSRSRYNPDTSGKN
Sbjct: 1087 FEVSSKHLKGGKECKVDVLVMENLLFRHNVTRLYDLKGSSRSRYNPDTSGKN 1138

HSP 3 Score: 1208.4 bits (3125), Expect = 0.0e+00
Identity = 579/594 (97.47%), Postives = 585/594 (98.48%), Query Frame = 1

Query: 1   MGHPENKISDLVGLVKSWMPWRSDPANVSRDFWMPDKSCRVCYECDSQFTFINRRHHCRR 60
           MGHPENKISDLVGLVKSWMPWRSDP NVSRDFWMPDKSCRVCYECDSQFTFINRRHHCRR
Sbjct: 1   MGHPENKISDLVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYECDSQFTFINRRHHCRR 60

Query: 61  CGRVFCSKCTANFIPALSTDPTNAREDSEKIRVCNYCFKQWGKEPSSLQDGDKATSSALS 120
           CGRVFCS+CTANFIPA STD TNAREDSEKIRVCNYCFKQWGKEPSSLQDGDKATSSALS
Sbjct: 61  CGRVFCSRCTANFIPAPSTDATNAREDSEKIRVCNYCFKQWGKEPSSLQDGDKATSSALS 120

Query: 121 VSSSSTSMGSTKSGYTCHSAISHIASTPCSTSRQYYDPYCSAAIADEHDNLRNGKTINRT 180
           +SSSSTSMGSTKSGYTCHSAISHIASTPCST RQYYDPYCSAA+ADEHDNLRNGKTINRT
Sbjct: 121 LSSSSTSMGSTKSGYTCHSAISHIASTPCSTGRQYYDPYCSAAMADEHDNLRNGKTINRT 180

Query: 181 TSLMTSSSSYCGYYRSDDEDDDYYYGMYRSDSESRHISQSDDYYCAVNIDGGLNLERENT 240
           TSLMTSSSSY GYYRSDDEDDDYYYGMYRSDSESRHISQSDDYYCAVNID G NLERENT
Sbjct: 181 TSLMTSSSSYYGYYRSDDEDDDYYYGMYRSDSESRHISQSDDYYCAVNIDEGSNLERENT 240

Query: 241 STKGSPVNFDTQCPRGNEESSEDSDENDKGSECDFPTYAMQNKDHEPMDFQNNGILWLPP 300
           STKGSPVNFDTQ PRGNEESSEDSDENDKGSECDFP+YAMQNK+HEPMDFQNNGILWLPP
Sbjct: 241 STKGSPVNFDTQSPRGNEESSEDSDENDKGSECDFPSYAMQNKNHEPMDFQNNGILWLPP 300

Query: 301 EPEDEEDEKALLFEDDDDVCAPGEWGHLHSSLSSGECPMKGKSSEDYKKTAKNVVEGHFR 360
           EPEDEEDEKALLFEDDDDVCAPGEWG+LHSSLSSGECPMKGKSSEDYKKTAKNVVEGHFR
Sbjct: 301 EPEDEEDEKALLFEDDDDVCAPGEWGYLHSSLSSGECPMKGKSSEDYKKTAKNVVEGHFR 360

Query: 361 ALVSQLLEAENLPIGDAPNEDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYVKVKCVA 420
           ALVSQLLEAENLPIGDAP EDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYVKVKCVA
Sbjct: 361 ALVSQLLEAENLPIGDAPREDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYVKVKCVA 420

Query: 421 GGKRSESIIVKGIICKKNVAHRRMTRGISKPRFFVLGGALEYQRVTNHLSSFDTLLQQEL 480
           GGKRSESIIVKGIICKKNVAHRRMTRGISKPRFFVLGGALEYQRVTNHLSSFDTLLQQEL
Sbjct: 421 GGKRSESIIVKGIICKKNVAHRRMTRGISKPRFFVLGGALEYQRVTNHLSSFDTLLQQEL 480

Query: 481 DHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCTGANII 540
           DHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCTGANII
Sbjct: 481 DHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCTGANII 540

Query: 541 PTVDHVASLKLGYCDAFRVETFVEEHDSGDGQGGKKSTKTLMFFEGCPKPLCCT 595
           PTVDHVASLKLGYCDAFRVETFVEEHDSGDGQGGKKSTKTLMFFEGCPKPLCCT
Sbjct: 541 PTVDHVASLKLGYCDAFRVETFVEEHDSGDGQGGKKSTKTLMFFEGCPKPLCCT 594

BLAST of MELO3C010117 vs. NCBI nr
Match: gi|778664198|ref|XP_011660241.1| (PREDICTED: 1-phosphatidylinositol-3-phosphate 5-kinase FAB1B-like isoform X1 [Cucumis sativus])

HSP 1 Score: 981.1 bits (2535), Expect = 1.9e-282
Identity = 492/512 (96.09%), Postives = 501/512 (97.85%), Query Frame = 1

Query: 801  ERLNYVATLRKKHSPDGSSNDISTLEEKELNSLEELVELTLASGRDEFFGGCDNDSLPPE 860
            +RLNYVATLRKKHSPDGSSNDIS L EKELNSLEELVELTLASGRDEFFGGCDNDSLP E
Sbjct: 1127 QRLNYVATLRKKHSPDGSSNDISILVEKELNSLEELVELTLASGRDEFFGGCDNDSLPAE 1186

Query: 861  SKREAGLGREGNSGNISRLNETNREKNIDLGGQEDDFCLSPVNSEPVDVGIGIRRANSVG 920
            SKREAGLGREGNSGNISRLN+T+REKNIDLGGQEDDFCLSPVN +PVDVGIGIRRANSVG
Sbjct: 1187 SKREAGLGREGNSGNISRLNKTDREKNIDLGGQEDDFCLSPVNLDPVDVGIGIRRANSVG 1246

Query: 921  EYPIMTDLSGTLDAVWKGVIHPNSLIFEDSFSRTNSAILEPLAGQSDMEKCTANGIETAH 980
            EYPIMT + GTLDAVWKGVIHPNSLIF+DSFSRT SAILEPLAGQSDMEKCTAN IETAH
Sbjct: 1247 EYPIMT-VPGTLDAVWKGVIHPNSLIFDDSFSRTTSAILEPLAGQSDMEKCTANRIETAH 1306

Query: 981  LLHSALVLTSDDSVETITNSTSMPPSAYNTKISLLNIPKSDISDYSSVFISSYRELEKQS 1040
            LLHSALVLT DDSVETI NSTSMPPSAYNTKISLLNIPKSDISDYSSVFISSYRELEKQS
Sbjct: 1307 LLHSALVLTRDDSVETIPNSTSMPPSAYNTKISLLNIPKSDISDYSSVFISSYRELEKQS 1366

Query: 1041 HLRLLMHVGSSDTVVPVYDEEPTSIIAYSLLMPEYIAQMSEPVKGMEASDTARSLPVIGS 1100
            HLRLLMHVGSSDTVVPVYDEEPTSIIAYS+LMPEYIAQMSEP KGMEASDTARSLPVIGS
Sbjct: 1367 HLRLLMHVGSSDTVVPVYDEEPTSIIAYSMLMPEYIAQMSEPAKGMEASDTARSLPVIGS 1426

Query: 1101 VKMNSIKTCDEEGSDAYKTLRSNEETILSMPRTESLQFEDLVCMTKDLHTRVCFVNETPL 1160
            VKMNSIKTCDEEGSDAYKTLRSNEETILSMPRTESLQFEDLVCM+KDLHTRVCF+NETPL
Sbjct: 1427 VKMNSIKTCDEEGSDAYKTLRSNEETILSMPRTESLQFEDLVCMSKDLHTRVCFINETPL 1486

Query: 1161 GPVKYTITCYFAKRFEALRKKCCPSELDFVRSLSRCRRWGAQGGKSNVFFAKTLDERFII 1220
            GPVKYTITCYFAKRFEALRKKCCPSELDFVRSLSRCRRWGAQGGKSNVFFAKTLDERFII
Sbjct: 1487 GPVKYTITCYFAKRFEALRKKCCPSELDFVRSLSRCRRWGAQGGKSNVFFAKTLDERFII 1546

Query: 1221 KQVTKIELESFIQFSKAYFIYLSQAICTGCPTCLAKVVGMYQVSSKHLKGGKECKVDVLV 1280
            KQVTKIELESFIQFSKAYFIYLSQAICTGCPTCLAKVVGMYQVSSKHLKGGKECKVDVLV
Sbjct: 1547 KQVTKIELESFIQFSKAYFIYLSQAICTGCPTCLAKVVGMYQVSSKHLKGGKECKVDVLV 1606

Query: 1281 MENLLFRHNVTRLYDLKGSSRSRYNPDTSGKN 1313
            MENLLFRHNVTRLYDLKGSSRSRYNPDTSGKN
Sbjct: 1607 MENLLFRHNVTRLYDLKGSSRSRYNPDTSGKN 1637

HSP 2 Score: 438.7 bits (1127), Expect = 3.5e-119
Identity = 204/207 (98.55%), Postives = 204/207 (98.55%), Query Frame = 1

Query: 595  NYRCHSCGMPSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGF 654
            NYRCHSCGMPSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGF
Sbjct: 882  NYRCHSCGMPSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGF 941

Query: 655  PPATRRVIMSDAAWGLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFH 714
            PPATRRVIMSDAAWGLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFH
Sbjct: 942  PPATRRVIMSDAAWGLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFH 1001

Query: 715  YAAINVQSVHIPPSMIEFHLNNLEWINKEANEVHNRAKTLFDEVCKALPLISKNESGAEQ 774
            YAAINVQSVHIPPSMIEFHLNNLEWINKEANEVHNRAKTLF EVCKALPLI KNESGAEQ
Sbjct: 1002 YAAINVQSVHIPPSMIEFHLNNLEWINKEANEVHNRAKTLFGEVCKALPLIPKNESGAEQ 1061

Query: 775  EDFGADELNNYIQELEQIINMEKENFE 802
            EDFG DELNNYIQELEQIINMEKENFE
Sbjct: 1062 EDFGVDELNNYIQELEQIINMEKENFE 1088

HSP 3 Score: 746.9 bits (1927), Expect = 6.0e-212
Identity = 381/621 (61.35%), Postives = 463/621 (74.56%), Query Frame = 1

Query: 1   MGHPENKISDLVGLVKSWMPWRSDPANVSRDFWMPDKSCRVCYECDSQFTFINRRHHCRR 60
           MG+P+NK+SDLV +VKSW+P RS+P NVSRDFWMPD+SCRVCYECDSQFT  NRRHHCR 
Sbjct: 1   MGNPDNKLSDLVDIVKSWIPRRSEPPNVSRDFWMPDQSCRVCYECDSQFTVFNRRHHCRL 60

Query: 61  CGRVFCSKCTANFIPALSTDPTNAREDSEKIRVCNYCFKQWGKEPSSLQDGDKATSSALS 120
           CGRVFC+KCTAN +PA S      +EDSE+IRVCNYCFKQW +  +++  G  A S  LS
Sbjct: 61  CGRVFCAKCTANSVPAPSDVQRAGQEDSERIRVCNYCFKQWEQWIAAVDTGTNAHSPGLS 120

Query: 121 VSSSSTSMGSTKSGYTCHSAISHIASTPCSTSRQYYDPYCSA----------AIADEHDN 180
            S S+TS+ STKS  TC+S+ S + STP ST   +   Y S           A A E +N
Sbjct: 121 PSPSATSLASTKSSCTCNSSSSTVGSTPYSTGPYHRVNYNSGLSPRESSQMNASATEQNN 180

Query: 181 LRNGKTINRTTSLMTSSSSYCGYY--RSDDEDDDYYYGMYRSDSESRHISQSDDYYCAVN 240
             +G + N +++ + SSS++ G    RSDDEDDDY  G Y SDSESRH + ++DYY A+N
Sbjct: 181 KASGTSTNPSSAAVDSSSNHFGLCDNRSDDEDDDY--GAYHSDSESRHYAHAEDYYGAIN 240

Query: 241 I--------------DGGLNLERENTSTKGSPVNFDTQCPRGNEESSEDSDENDKGSECD 300
           I              DGG N++ ++ S    P NF+ Q   G ++  E+ +E +   E +
Sbjct: 241 IGSIDRVYGSDKVHPDGG-NMDTKSLSGSPLPENFNAQSVDGIKKF-EEVNERENADEGE 300

Query: 301 FPTYAMQNKDHEPMDFQNNGILWLPPEPEDEEDEK--ALLFEDDDDVCAPGEWGHLHSSL 360
            P Y +   D EP+DF+NNG+LWLPPEPEDEEDE+  AL  +DDDD  A GEWG+L SS 
Sbjct: 301 VPAYDVDGTDVEPVDFENNGLLWLPPEPEDEEDERESALFDDDDDDEGASGEWGYLRSSN 360

Query: 361 S--SGECPMKGKSSEDYKKTAKNVVEGHFRALVSQLLEAENLPIGDAPNEDGWLDIITYL 420
           S  SGE   + KS+E++++  KNVVEGHFRALV+QLL+ ENLP+GD    D WLDIITYL
Sbjct: 361 SFGSGEYRSRDKSNEEHRRAMKNVVEGHFRALVAQLLQVENLPVGDEDGGDSWLDIITYL 420

Query: 421 SWEAAAVLKPDTSKSGGMDPGGYVKVKCVAGGKRSESIIVKGIICKKNVAHRRMTRGISK 480
           SWEAA +LKPDTSK GGMDPGGYVKVKC+A G+R+ES +VKG++CKKNVAHRRMT  I K
Sbjct: 421 SWEAATLLKPDTSKGGGMDPGGYVKVKCIASGRRNESSVVKGVVCKKNVAHRRMTSKIDK 480

Query: 481 PRFFVLGGALEYQRVTNHLSSFDTLLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQE 540
           PRF +LGGALEYQR+++HLSSFDTLLQQE+DHLKMAVAKIDAHHPNVLLVEKSVSRHAQE
Sbjct: 481 PRFLILGGALEYQRISSHLSSFDTLLQQEMDHLKMAVAKIDAHHPNVLLVEKSVSRHAQE 540

Query: 541 YLLSKNITLVLNIKRSLLERIACCTGANIIPTVDHVASLKLGYCDAFRVETFVEEHDSGD 592
           YLL+K+I+LVLNIKR LLERIA CTGA I+P++DH+ S KLGYCD F VE F+EEH S  
Sbjct: 541 YLLAKDISLVLNIKRPLLERIARCTGAQIVPSIDHLTSPKLGYCDVFHVEKFLEEHGSA- 600

BLAST of MELO3C010117 vs. NCBI nr
Match: gi|590574831|ref|XP_007012516.1| (Phosphatidylinositol-4-phosphate 5-kinase family protein, putative isoform 2, partial [Theobroma cacao])

HSP 1 Score: 701.4 bits (1809), Expect = 2.9e-198
Identity = 392/774 (50.65%), Postives = 520/774 (67.18%), Query Frame = 1

Query: 595  NYRCHSCGMPSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGF 654
            ++RC SC MPSEAHVHCYTH QGSLTIS +KLP+  LPG+ EGKIWMWHRCL+CPR N F
Sbjct: 924  SFRCRSCEMPSEAHVHCYTHRQGSLTISVRKLPELPLPGQREGKIWMWHRCLRCPRANKF 983

Query: 655  PPATRRVIMSDAAWGLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFH 714
            PPATRR++MSDAAWGLSFGKFLELSFSN+AAASRVA CGH LHRDCLRFYGFGR VACF 
Sbjct: 984  PPATRRIVMSDAAWGLSFGKFLELSFSNHAAASRVASCGHSLHRDCLRFYGFGRRVACFR 1043

Query: 715  YAAINVQSVHIPPSMIEFHLNNLEWINKEANEVHNRAKTLFDEVCKALPLISKN------ 774
            YAAI+V SV++PP  +EF+ +N EWI  EANEV NRA+ LF EV  AL  +S+       
Sbjct: 1044 YAAIDVHSVYLPPPKLEFNYDNQEWIQSEANEVTNRAEFLFREVYNALQKMSEKLLGPGF 1103

Query: 775  -ESGAEQ-------EDFGA------DELNNYIQEL--------EQIIN-MEKENFER--- 834
             + G +        E+  A      +E    +QE+        + +I+ +E    +R   
Sbjct: 1104 QDGGIKSPEKRICIEELEAMLQKDREEFQESLQEVLCKEVKVGQPVIDILEINKLQRQIL 1163

Query: 835  -LNYVATLRKKHS--------PDGSSNDISTLEEKELNSLEELVELTLASGRDEFFGGCD 894
             L+YV   R  H+         +  S+ I  L  K ++S+E+LVE+ ++    +    CD
Sbjct: 1164 FLSYVWDQRLIHAFSSIVNNIQEVMSSSIPKLGLKPVSSVEKLVEINVSPKPSKALSSCD 1223

Query: 895  NDSLPPESKREAGLGREGNSGNISRLNETNREKNIDL---GGQEDDFCLS-----PVNSE 954
            +  +  ++K +  + +EGN+G IS     +REK +D       E +  LS        S+
Sbjct: 1224 SALV--QTKPDININQEGNTGEISEPGGDHREKGMDQDLNSRNEAESSLSCSANTSEKSD 1283

Query: 955  PVDVGIGIRRANSVGEYPIMTDLSGTLDAVWKGVIHPNSL-IFEDSFSRTNSAILE-PLA 1014
             ++ G  +RRA S GE+PIM +LS TL+A W G  HP S+   E+ +S +++ +++   A
Sbjct: 1284 SLESGKVVRRALSEGEFPIMANLSDTLEAAWTGESHPASVGPKENGYSVSDTVVVDLSTA 1343

Query: 1015 GQSDMEKCTANG--IETAHLLHSALVLTSDDSVETITNSTSMP-PSAYN--TKISLLNIP 1074
              SDM   T++   +E A    SAL     +++E   +  SMP P+ Y+   K S  N  
Sbjct: 1344 ANSDMGNRTSDRGEVEVACSPQSALPTKGPENMEKTMSWASMPFPNFYSLFNKNSSFNAQ 1403

Query: 1075 KSDISDYSSVFISSYRELEKQSHLRLLMHVGSSDTVVPVYDEEPTSIIAYSLLMPEYIAQ 1134
            K  IS+Y+ V++SS RELE+QS  RLL+ +G +DTVVPVYD+EPTSIIAY+L+  +Y +Q
Sbjct: 1404 KLSISEYNPVYVSSLRELERQSGARLLLPIGVNDTVVPVYDDEPTSIIAYALVSSDYYSQ 1463

Query: 1135 MSEPVKGMEASDTARSLPVIGSVKMNSIKTCDEEGSDAYKTLRSNEETILSMPRTESLQF 1194
            MSE  K  +A+D+A S  +  SV +  + + ++  SD +++  S +E+ILS+  + S   
Sbjct: 1464 MSELEKPKDAADSAVSSSLFDSVNLLLLNSFNDSSSDTFRSFGSGDESILSISGSYSSLV 1523

Query: 1195 EDLVCMTKDLHTRVCFVNETPLGPVKYTITCYFAKRFEALRKKCCPSELDFVRSLSRCRR 1254
             D +  TK+ H RV F ++ PLG VK+++TCY+AK FE+LR+ CCPSELDF+RSLSRC++
Sbjct: 1524 SDPLLDTKNFHARVSFTDDGPLGKVKHSVTCYYAKWFESLRRTCCPSELDFIRSLSRCKK 1583

Query: 1255 WGAQGGKSNVFFAKTLDERFIIKQVTKIELESFIQFSKAYFIYLSQAICTGCPTCLAKVV 1313
            WGAQGGKSNVFFAKTLD+RFIIKQVTK ELESFI+F  AYF YLS +I T  PTCLAK++
Sbjct: 1584 WGAQGGKSNVFFAKTLDDRFIIKQVTKTELESFIKFGPAYFKYLSDSISTRSPTCLAKIL 1643

HSP 2 Score: 746.9 bits (1927), Expect = 6.0e-212
Identity = 381/621 (61.35%), Postives = 463/621 (74.56%), Query Frame = 1

Query: 1   MGHPENKISDLVGLVKSWMPWRSDPANVSRDFWMPDKSCRVCYECDSQFTFINRRHHCRR 60
           MG+P+NK+SDLV +VKSW+P RS+P NVSRDFWMPD+SCRVCYECDSQFT  NRRHHCR 
Sbjct: 1   MGNPDNKLSDLVDIVKSWIPRRSEPPNVSRDFWMPDQSCRVCYECDSQFTVFNRRHHCRL 60

Query: 61  CGRVFCSKCTANFIPALSTDPTNAREDSEKIRVCNYCFKQWGKEPSSLQDGDKATSSALS 120
           CGRVFC+KCTAN +PA S      +EDSE+IRVCNYCFKQW +  +++  G  A S  LS
Sbjct: 61  CGRVFCAKCTANSVPAPSDVQRAGQEDSERIRVCNYCFKQWEQWIAAVDTGTNAHSPGLS 120

Query: 121 VSSSSTSMGSTKSGYTCHSAISHIASTPCSTSRQYYDPYCSA----------AIADEHDN 180
            S S+TS+ STKS  TC+S+ S + STP ST   +   Y S           A A E +N
Sbjct: 121 PSPSATSLASTKSSCTCNSSSSTVGSTPYSTGPYHRVNYNSGLSPRESSQMNASATEQNN 180

Query: 181 LRNGKTINRTTSLMTSSSSYCGYY--RSDDEDDDYYYGMYRSDSESRHISQSDDYYCAVN 240
             +G + N +++ + SSS++ G    RSDDEDDDY  G Y SDSESRH + ++DYY A+N
Sbjct: 181 KASGTSTNPSSAAVDSSSNHFGLCDNRSDDEDDDY--GAYHSDSESRHYAHAEDYYGAIN 240

Query: 241 I--------------DGGLNLERENTSTKGSPVNFDTQCPRGNEESSEDSDENDKGSECD 300
           I              DGG N++ ++ S    P NF+ Q   G ++  E+ +E +   E +
Sbjct: 241 IGSIDRVYGSDKVHPDGG-NMDTKSLSGSPLPENFNAQSVDGIKKF-EEVNERENADEGE 300

Query: 301 FPTYAMQNKDHEPMDFQNNGILWLPPEPEDEEDEK--ALLFEDDDDVCAPGEWGHLHSSL 360
            P Y +   D EP+DF+NNG+LWLPPEPEDEEDE+  AL  +DDDD  A GEWG+L SS 
Sbjct: 301 VPAYDVDGTDVEPVDFENNGLLWLPPEPEDEEDERESALFDDDDDDEGASGEWGYLRSSN 360

Query: 361 S--SGECPMKGKSSEDYKKTAKNVVEGHFRALVSQLLEAENLPIGDAPNEDGWLDIITYL 420
           S  SGE   + KS+E++++  KNVVEGHFRALV+QLL+ ENLP+GD    D WLDIITYL
Sbjct: 361 SFGSGEYRSRDKSNEEHRRAMKNVVEGHFRALVAQLLQVENLPVGDEDGGDSWLDIITYL 420

Query: 421 SWEAAAVLKPDTSKSGGMDPGGYVKVKCVAGGKRSESIIVKGIICKKNVAHRRMTRGISK 480
           SWEAA +LKPDTSK GGMDPGGYVKVKC+A G+R+ES +VKG++CKKNVAHRRMT  I K
Sbjct: 421 SWEAATLLKPDTSKGGGMDPGGYVKVKCIASGRRNESSVVKGVVCKKNVAHRRMTSKIDK 480

Query: 481 PRFFVLGGALEYQRVTNHLSSFDTLLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQE 540
           PRF +LGGALEYQR+++HLSSFDTLLQQE+DHLKMAVAKIDAHHPNVLLVEKSVSRHAQE
Sbjct: 481 PRFLILGGALEYQRISSHLSSFDTLLQQEMDHLKMAVAKIDAHHPNVLLVEKSVSRHAQE 540

Query: 541 YLLSKNITLVLNIKRSLLERIACCTGANIIPTVDHVASLKLGYCDAFRVETFVEEHDSGD 592
           YLL+K+I+LVLNIKR LLERIA CTGA I+P++DH+ S KLGYCD F VE F+EEH S  
Sbjct: 541 YLLAKDISLVLNIKRPLLERIARCTGAQIVPSIDHLTSPKLGYCDVFHVEKFLEEHGSA- 600