MELO3C009917 (gene) Melon (DHL92) v3.5.1

BLAST of MELO3C009917 vs. Swiss-Prot
Match: MTP12_ARATH (Metal tolerance protein 12 OS=Arabidopsis thaliana GN=MTP12 PE=3 SV=1)

HSP 1 Score: 286.2 bits (731), Expect = 1.0e-75
Identity = 140/164 (85.37%), Postives = 152/164 (92.68%), Query Frame = 1

Query: 265 VESSDSIVTMVMRPVRHILTERKSRKIALFLLINTGYMVVEFVAGFMSNSLGLISDACHM 324
           +ES +S  TM M+P+RHIL+E+KSRKIALFLLINT YMVVEFVAGFMSNSLGLISDACHM
Sbjct: 1   MESPESFSTMFMKPIRHILSEKKSRKIALFLLINTAYMVVEFVAGFMSNSLGLISDACHM 60

Query: 325 LFDCAALAIGLYASYISRLPANNQFNYGRGRFEILSGYANAVFLVLVGALIVLESLERIL 384
           LFDCAALAIGLYASYISRLPAN+Q+NYGRGRFE+LSGY NAVFLVLVGALIVLES+ERIL
Sbjct: 61  LFDCAALAIGLYASYISRLPANHQYNYGRGRFEVLSGYVNAVFLVLVGALIVLESIERIL 120

Query: 385 DPQEISTSSLLTVSIGGLVVNVVGLIFFHEEHHHAHGGSGSCSH 429
           DPQEIST+SLL VS+GGL+VN+VGLIFFHEEHHHAHGGSG   H
Sbjct: 121 DPQEISTNSLLVVSVGGLLVNIVGLIFFHEEHHHAHGGSGIFLH 164

HSP 2 Score: 212.6 bits (540), Expect = 1.5e-53
Identity = 108/148 (72.97%), Postives = 119/148 (80.41%), Query Frame = 1

Query: 589 HHHHHIDHNMEGIFLHVLADTMGSVGVVISTLLIKYKGWLVADPACSIFISIMIISSVIP 648
           H  HH  H   GIFLHVLADTMGSVGVVISTLLIKYKGWLVADPA SIFISI+II+SVIP
Sbjct: 149 HEEHHHAHGGSGIFLHVLADTMGSVGVVISTLLIKYKGWLVADPASSIFISILIIASVIP 208

Query: 649 LLRNSAEILLQRVPRAHEQDLKEAVSDIMEIHGVQGIQNLHVWSFTNTDVVGTLRLHVST 708
           LLRNSAEILLQRVPRAH QDLKEA+ +I++  GV  IQ LHVWSFTN+DVV TL L VS 
Sbjct: 209 LLRNSAEILLQRVPRAHRQDLKEAMRNILKTKGVCSIQRLHVWSFTNSDVVATLHLLVSA 268

Query: 709 ETDKPSIKAKVEHILHDAGIKDLTLQLE 737
           ++DK   K +V  +L DAG+KD TLQ+E
Sbjct: 269 DSDKTDTKLQVSRLLEDAGVKDWTLQVE 296

BLAST of MELO3C009917 vs. Swiss-Prot
Match: MTP8_ORYSJ (Metal tolerance protein 8 OS=Oryza sativa subsp. japonica GN=MTP8 PE=2 SV=2)

HSP 1 Score: 266.9 bits (681), Expect = 6.5e-70
Identity = 135/184 (73.37%), Postives = 149/184 (80.98%), Query Frame = 1

Query: 276 MRPVRHILTERKSRKIALFLLINTGYMVVEFVAGFMSNSLGLISDACHMLFDCAALAIGL 335
           M PVRHIL ERKSRKIA FLLINT YM VEF +GFMS+SLGLISDACHMLFDCAALAIGL
Sbjct: 1   MGPVRHILNERKSRKIAAFLLINTAYMFVEFTSGFMSDSLGLISDACHMLFDCAALAIGL 60

Query: 336 YASYISRLPANNQFNYGRGRFEILSGYANAVFLVLVGALIVLESLERILDPQEISTSSLL 395
           YASYI+RLPAN  +NYGRGRFE+LSGY NAVFLVLVGALIVLES ERIL+P+EISTSSLL
Sbjct: 61  YASYIARLPANGLYNYGRGRFEVLSGYVNAVFLVLVGALIVLESFERILEPREISTSSLL 120

Query: 396 TVSIGGLVVNVVGLIFFHEEHHHAHGGSGSCSHSHSHSHSQRNSHSCAGSHHHDKHEHDS 455
           TVSIGGLVVNV+GL+FFHEEHHHAHG + SC+     S     +H+ + + HH  H  + 
Sbjct: 121 TVSIGGLVVNVIGLVFFHEEHHHAHGEAHSCNGGLQSS----ENHNKSRNRHHIDHNMEG 180

Query: 456 CRKH 460
              H
Sbjct: 181 IFLH 180

HSP 2 Score: 237.3 bits (604), Expect = 5.5e-61
Identity = 113/173 (65.32%), Postives = 135/173 (78.03%), Query Frame = 1

Query: 570 ESSHSHPSKQPVGGTVRKKHHH------HHIDHNMEGIFLHVLADTMGSVGVVISTLLIK 629
           E  H+H       G ++   +H      HHIDHNMEGIFLHVLADTMGSVGVVISTLLIK
Sbjct: 140 EHHHAHGEAHSCNGGLQSSENHNKSRNRHHIDHNMEGIFLHVLADTMGSVGVVISTLLIK 199

Query: 630 YKGWLVADPACSIFISIMIISSVIPLLRNSAEILLQRVPRAHEQDLKEAVSDIMEIHGVQ 689
           YKGWL+ADP CS+FISIMI+SSV+PLLRNSAEILLQRVPR+ E+D+KEA+ D+M+I GV 
Sbjct: 200 YKGWLIADPICSVFISIMIVSSVLPLLRNSAEILLQRVPRSLEKDIKEALDDVMKIKGVI 259

Query: 690 GIQNLHVWSFTNTDVVGTLRLHVSTETDKPSIKAKVEHILHDAGIKDLTLQLE 737
           G+ N HVW+ TNTD+VGT  LH++TE DK SI+ K   I H+AGI+DLT+Q+E
Sbjct: 260 GVHNFHVWNLTNTDIVGTFHLHITTEADKSSIREKASDIFHEAGIQDLTIQIE 312

BLAST of MELO3C009917 vs. Swiss-Prot
Match: CIS4_SCHPO (Probable zinc transporter cis4 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=cis4 PE=1 SV=1)

HSP 1 Score: 258.5 bits (659), Expect = 2.3e-67
Identity = 156/461 (33.84%), Postives = 242/461 (52.49%), Query Frame = 1

Query: 279 VRHILTERKSRKIALFLLINTGYMVVEFVAGFMSNSLGLISDACHMLFDCAALAIGLYAS 338
           V  +L +++SR I  F L+N  YM V+ + G  +NSLGLISDA HM FDC A+ +GL A+
Sbjct: 338 VNGVLADKESRSIFYFFLLNVSYMFVQVIYGLWTNSLGLISDAIHMAFDCIAILVGLVAT 397

Query: 339 YISRLPANNQFNYGRGRFEILSGYANAVFLVLVGALIVLESLERILDPQEISTSSLLTVS 398
            ++++P N  + +G  + E LSG+ N +FLVL+   IV E+L R+  P +++T  LL VS
Sbjct: 398 TLAKMPLNYAYPFGFAKIEALSGFTNGIFLVLISFSIVGEALYRLFHPPQMNTDQLLLVS 457

Query: 399 IGGLVVNVVGLIFFHEEHHHAHGGSGSCSHSHSHSHSQRNSHSCAGSHHHDKHEHDSCRK 458
             GLVVN+VG++ F+  H+H HG     SH                 HHH    H  C  
Sbjct: 458 FLGLVVNLVGILAFNHGHNHDHG-----SH-----------------HHHSHSNHSMCLP 517

Query: 459 HE-NAISVTKECHESSVSVHTGHHEHRQVDVCSENHLSNHCDDHHHLEHTDHHDHKHDHS 518
           +  N I++ +E  E   +V     E +++   +++H+S H       EHT          
Sbjct: 518 NTTNDINIFEEFEEEKDNV-----EAQKMGYTNDDHVSQH-------EHT---------- 577

Query: 519 HVHHHKHSHDHDHDHHHDCKHEHHDHVHRHDQHGHLSQPKSDVPKMVSHSVSESSHSHPS 578
               H++S +H H+H+H     +HDH+H++++       K D   +   ++    H    
Sbjct: 578 ----HENSQEHHHEHNH-----NHDHIHKYNE-------KCDHESISLQNLDNDHHC--- 637

Query: 579 KQPVGGTVRKKHHHHHIDHNMEGIFLHVLADTMGSVGVVISTLLIKYKGWLVADPACSIF 638
                       HHHH +HNM GIFLH++ADTMGSVGV++ST+LI++  W   DP  S+ 
Sbjct: 638 ------------HHHHENHNMHGIFLHIIADTMGSVGVIVSTILIQWFSWTGFDPLASLI 697

Query: 639 ISIMIISSVIPLLRNSAEILLQRVPRAHEQDLKEAVSDIMEIHGVQGIQNLHVWSFTNTD 698
           I+ +I  SV+PL+++SA+ LL       E  LK+ +S+I   + V  + N   W+    +
Sbjct: 698 IAALIFVSVLPLIKDSAKNLLSVTDPESEYLLKQCLSNISLSNSVISLSNPKFWTNERGE 723

Query: 699 VVGTLRLHVSTETDKPSIKAKVEHILHDA--GIKDLTLQLE 737
           V G L + VS + D   ++ +V   L  A   +K + +Q E
Sbjct: 758 VYGILHIQVSIDGDLNVVRNEVFRKLSIAVPNLKHICIQSE 723

BLAST of MELO3C009917 vs. Swiss-Prot
Match: Y1141_DICDI (Probable zinc transporter protein DDB_G0291141 OS=Dictyostelium discoideum GN=DDB_G0291141 PE=3 SV=1)

HSP 1 Score: 173.7 bits (439), Expect = 7.5e-42
Identity = 132/485 (27.22%), Postives = 222/485 (45.77%), Query Frame = 1

Query: 5   QLKTMVVKSVLLAIVFLLRFQALLYCGTAAMILA-EMTGNVAARFLVEGRNQMTMGDRTR 64
           Q+K ++  S+   ++ +    ++ + G    ILA + T +          N +  G+   
Sbjct: 97  QIKKIIYHSIFNLLIIITWNSSIKFIGPIGSILASDYTFSTYPLIF----NSLLQGNFLA 156

Query: 65  SRSSEVRGFLSLFFGLFLLSISW--DRIDCFPFATSFIDKYGFSVLPRENCMRIWPMLL- 124
           +  S  RG + L  G FL+ +    +R+D   + +S +   G   L   N + +W   + 
Sbjct: 157 TDMS--RGSIMLMIGYFLIPLFGISNRLDILGYTSSQVFMIGLFSLIVHNVLVLWKKTIV 216

Query: 125 -PFLSGFLGCYERISMNWGSVKQL----------------GQKRVRLVS---LFFTTIIL 184
             + SG  G   ++S     V  +                G   +  VS   LF   II 
Sbjct: 217 RSWNSGSSGGKNKLSSLGSCVSTIILFVFKLFEGFSSGSSGSDSINQVSYSQLFVIAIIT 276

Query: 185 FVPAVISMLLFEAEGKSVSLGSLAW-PLANTVVFGVLLNENYSDDKLVSSKDFRSEFLVT 244
           F+   ++  + +   K ++   L+   L ++V+FG+L          +  KDF    L+ 
Sbjct: 277 FILYSLNQFIDDVSEKELTFNVLSKVSLTSSVIFGLLAA------LFIGFKDFFHPILIL 336

Query: 245 FVCTVI--LELLYFPELSLWGLLFCGLLLYVAVRELDPVFLNYHELGVESSDSIVT---- 304
               +I  + +LY     +  + F   +        +     Y+  G     SI+     
Sbjct: 337 SFIFIINAIHILYSKSNDIQPMTFSNNM-----DGGNSSIKTYNSSGGGGGGSIINGNGS 396

Query: 305 --------MVMRPVRHILTERKSRKIALFLLINTGYMVVEFVAGFMSNSLGLISDACHML 364
                   ++   +R I+ +  SR+I  FL+IN  +M VE   G  +NSLGLI+DACHM 
Sbjct: 397 GNAIYYFEILKDVLRQIVDKPTSRRIFTFLVINLMFMFVEMAYGIWTNSLGLITDACHMF 456

Query: 365 FDCAALAIGLYASYISRLPANNQFNYGRGRFEILSGYANAVFLVLVGALIVLESLERILD 424
           FD  AL I L A  IS+   N++++YG GRF++LSG+ N +FL+ +   I++ES+ER+L+
Sbjct: 457 FDATALFIALVAEVISQWKQNDKYSYGYGRFQVLSGFVNGIFLIFIAVTILMESVERLLE 516

Query: 425 PQEISTSSLLTVSIGGLVVNVVGLIFFHEEHHHAHGGSGSCSHSHSHSHSQRNSHSCAGS 451
           P EI+T  LL VS+ G ++N++G+  FH +H H+HGG G  SH       + + HS  G 
Sbjct: 517 PPEINTDKLLLVSVLGFIINLIGIFSFHGDHGHSHGGGGGHSHGGGEKKEKHHGHSHGGH 564

HSP 2 Score: 115.2 bits (287), Expect = 3.2e-24
Identity = 74/227 (32.60%), Postives = 110/227 (48.46%), Query Frame = 1

Query: 527 DHDHDHHHDCKHEHHDHVHRHDQHGHLSQPKSDVPKMVSHSVSESSHS-HPSKQPVGGTV 586
           DH H H     H H     +   HGH                S   H  H    P+ G  
Sbjct: 529 DHGHSHGGGGGHSHGGGEKKEKHHGH----------------SHGGHGDHQQVTPILGEE 588

Query: 587 RKKHHHHHIDHNMEGIFLHVLADTMGSVGVVISTLLIKYKGWLVADPACSIFISIMIISS 646
           +KK        N++G+FLH+LADT+GSVGV++S+L+I+  G+ +ADP CS+ ISI+I  S
Sbjct: 589 KKKKR----SVNIDGVFLHLLADTLGSVGVIVSSLIIQIWGYTLADPICSLLISILIFLS 648

Query: 647 VIPLLRNSAEILLQRVPRAHEQDLKEAVSDIMEIHGVQGIQNLHVWSFTNTDVVGTLRLH 706
           V+PL+ N+A+ LLQ  P   +  L +    I+ I GV  I + H WS  +   + TL++ 
Sbjct: 649 VLPLIANTAKTLLQCTPEPIQSSLYQINQFILSIDGVHNIISYHFWSHYDDMNIATLKIQ 708

Query: 707 VS----------TETDKPSIKAKV--EHILHDAGIKDLTLQLEHNRE 741
           ++          TE  K SI   +  +H +H   I+ + L   +N +
Sbjct: 709 LNETASSNSTLDTERIKKSISKYLNKDHNIHKCIIEFIPLLYNNNNQ 735

BLAST of MELO3C009917 vs. Swiss-Prot
Match: ZNT5_CHICK (Zinc transporter 5 OS=Gallus gallus GN=SLC30A5 PE=2 SV=1)

HSP 1 Score: 172.6 bits (436), Expect = 1.7e-41
Identity = 87/195 (44.62%), Postives = 120/195 (61.54%), Query Frame = 1

Query: 266 ESSDSIVTMVMRPVRHILTERKSRKIALFLLINTGYMVVEFVAGFMSNSLGLISDACHML 325
           +SS S+   +   ++ IL E  SR+I  FL +N  +  VE   G  +NSLGLISD  HML
Sbjct: 391 QSSQSLPRFIKESLKQILEEYDSRQIFYFLCLNLAFTFVELFYGVWTNSLGLISDGFHML 450

Query: 326 FDCAALAIGLYASYISRLPANNQFNYGRGRFEILSGYANAVFLVLVGALIVLESLERILD 385
           FDC+AL +GL+A+ ++R  A   F+YG GR EILSG+ N +FL+++   + +ES+ R++D
Sbjct: 451 FDCSALVMGLFAALMTRWKATRIFSYGYGRVEILSGFINGLFLMVIAFFVFMESVARLVD 510

Query: 386 PQEISTSSLLTVSIGGLVVNVVGLIFFHEEHHHAHGGSGSCSHSHSHSHSQR-NSHSCAG 445
           P +I T+ L  VS+GGL+VN+VG+  F   H H+HG S    HSH HSHS   +SHS   
Sbjct: 511 PPDIDTNMLTPVSVGGLIVNLVGICAF--SHAHSHGASRGGCHSHEHSHSYHGHSHSHGH 570

Query: 446 SHHHDKHEHDSCRKH 460
            H H+ H H     H
Sbjct: 571 GHSHNDHGHSHGHSH 583

HSP 2 Score: 160.2 bits (404), Expect = 8.6e-38
Identity = 88/220 (40.00%), Postives = 122/220 (55.45%), Query Frame = 1

Query: 517 SHVHHHKHSHDHDHDHHHDCKHEHHDHVHRHDQHGHLSQPKSDVPKMVSHSVSESSHSHP 576
           SH H H  S    H H H   H +H H H H  HGH            SH+    SH H 
Sbjct: 538 SHAHSHGASRGGCHSHEHS--HSYHGHSHSHG-HGH------------SHNDHGHSHGHS 597

Query: 577 SKQPVGGTVRKKHHHHHIDHNMEGIFLHVLADTMGSVGVVISTLLIKYKGWLVADPACSI 636
                GG          ++ NM G+FLHVLADT+GSVGV++ST  I+  GWL+ADP CS+
Sbjct: 598 HVSSGGG----------MNTNMRGVFLHVLADTLGSVGVIVSTTFIQQFGWLIADPLCSL 657

Query: 637 FISIMIISSVIPLLRNSAEILLQRVPRAHEQDLKEAVSDIMEIHGVQGIQNLHVWSFTNT 696
           FI+ +I  SVIPLL+++ ++LL R+P   E+DL  A+  I +I GV   ++ H W  + +
Sbjct: 658 FIATLIFLSVIPLLKDACQVLLLRIPPEQEKDLHAALEKIQKIDGVISYRDPHFWCHSAS 717

Query: 697 DVVGTLRLHVSTETDKPSIKAKVEHILHDAGIKDLTLQLE 737
            V GT+ + V ++  +  I  +V  IL DAG+ +LT+Q+E
Sbjct: 718 VVAGTIHVQVVSDVMEQRIVQQVTAILKDAGVNNLTVQVE 732

BLAST of MELO3C009917 vs. TrEMBL
Match: W9RBJ6_9ROSA (Metal tolerance protein 12 OS=Morus notabilis GN=L484_010513 PE=4 SV=1)

HSP 1 Score: 1013.1 bits (2618), Expect = 1.8e-292
Identity = 509/760 (66.97%), Postives = 590/760 (77.63%), Query Frame = 1

Query: 1   MSQQQLKTMVVKSVLLAIVFLLRFQALLYCGTAAMILAEMTGNVAARFLVEGRNQMTMGD 60
           +SQ + + ++ +S+LLA+VFLLRFQAL YCGTAAMILAE+TGNVAARF+ EG+N    G+
Sbjct: 158 ISQSEHRALLARSILLAVVFLLRFQALRYCGTAAMILAELTGNVAARFVSEGKN----GN 217

Query: 61  RTRSRSSEVRGFLSLFFGLFLLSISWDRIDCFPFATSFIDKYGFSVLPRENCMRIWPMLL 120
                  +VRGF++LF GLFLLSISWDRI+CFPF+   +DK GF    + NC+RIWPMLL
Sbjct: 218 FADRYGFKVRGFVALFLGLFLLSISWDRIECFPFSAKSVDKVGFFTFLKGNCLRIWPMLL 277

Query: 121 PFLSGFLGCYERISMNWGSVKQLGQKRVRLVSLFFTTIILFVPAVISMLLFEAEGKSVSL 180
           PFLSGFL CYER+SMNW +++ LG KRVRL+SLFFTT++LF PAV+S+ +FE+E  +VS+
Sbjct: 278 PFLSGFLSCYERVSMNWRAIRHLGMKRVRLISLFFTTVLLFFPAVVSVFMFESEADNVSI 337

Query: 181 GSLAWPLANTVVFGVLLNENYSDDKLVSSKDFRSEFLVTFVCTVILELLYFPELSLWGLL 240
           G+L WPLANTVVFGVLL ENYSD+KLVSSKDFR EFLVTFVCT++LEL YFPELSLWGLL
Sbjct: 338 GNLVWPLANTVVFGVLLAENYSDEKLVSSKDFRREFLVTFVCTLVLELFYFPELSLWGLL 397

Query: 241 FCGLLLYVAVRELDPVFLNYHELGVESSDSIVTMVMRPVRHILTERKSRKIALFLLINTG 300
            CGLLLY+AVRELDP + NY ELG+ESS+S  ++VM+P+RHIL+ERKSRKIALFL+INTG
Sbjct: 398 LCGLLLYIAVRELDPFYSNYLELGMESSESFSSLVMKPIRHILSERKSRKIALFLMINTG 457

Query: 301 YMVVEFVAGFMSNSLGLISDACHMLFDCAALAIGLYASYISRLPANNQFNYGRGRFEILS 360
           YMVVEFVAGF SNSLGLISDACHMLFDCAALAIGLYASYISRLPANNQFNYGRGRFE+LS
Sbjct: 458 YMVVEFVAGFTSNSLGLISDACHMLFDCAALAIGLYASYISRLPANNQFNYGRGRFEVLS 517

Query: 361 GYANAVFLVLVGALIVLESLERILDPQEISTSSLLTVSIGGLVVNVVGLIFFHEEHHHAH 420
           GY NAVFLVLVG+LIVLESLERILDPQEIST+SLL VSIGGLVVN+VGLIFFHEEHHHAH
Sbjct: 518 GYVNAVFLVLVGSLIVLESLERILDPQEISTNSLLIVSIGGLVVNIVGLIFFHEEHHHAH 577

Query: 421 GGSGSCSHSHSHSHSQRNSHSCAGSHHHDKHEHDSCRKHENAISVTKECHESSVSVHTGH 480
           GGSGSCSHSHSHSH   + H     H H  H+H  C KHE  I V+ E HE   S H   
Sbjct: 578 GGSGSCSHSHSHSHDHTHHHQ--HLHDHVGHDHKGCGKHEEHIFVSHESHEKLCSHHDEK 637

Query: 481 HEHRQVDVCSENHLSNHCDDHHHLE----HTDHHDHKHDH---SHVHHHKHSHDHD---- 540
           H H Q   C+ +  S  C D +HL     H  HH H H H   +H HHH HS  HD    
Sbjct: 638 HGHSQ---CTNHSSSGSCKDDNHLGNNKCHDHHHGHAHQHESPNHAHHHDHSCQHDQLQS 697

Query: 541 --HDHHHDCK-------HEHHDHVHRHDQHGHLSQPKSDVPKMVSHSVSESSHSHPSKQP 600
             H  HH C        H++H HV+ HD H H SQ +        H     +HS   ++ 
Sbjct: 698 NKHHQHHHCDHSGHGELHDNHSHVNLHDHHDHASQHE-------CHGHGSHTHSFEVRKV 757

Query: 601 VGGTVR----KKHHHHHIDHNMEGIFLHVLADTMGSVGVVISTLLIKYKGWLVADPACSI 660
               V+     KHHHHHIDHNMEGIFLHVLADTMGSVGVVISTLLIKYKGWLVADPACSI
Sbjct: 758 AESQVQVEESNKHHHHHIDHNMEGIFLHVLADTMGSVGVVISTLLIKYKGWLVADPACSI 817

Query: 661 FISIMIISSVIPLLRNSAEILLQRVPRAHEQDLKEAVSDIMEIHGVQGIQNLHVWSFTNT 720
            IS++I+SSV+PLLRNSAEILLQR+PRAHE DL+ A+ +I ++ GV GIQNLHVWSFTNT
Sbjct: 818 CISVLIVSSVLPLLRNSAEILLQRIPRAHEVDLRRALIEIRKMRGVSGIQNLHVWSFTNT 877

Query: 721 DVVGTLRLHVSTETDKPSIKAKVEHILHDAGIKDLTLQLE 737
           DVVGTLRL V+ ET+K S K +   +L  AGIKDLTLQ+E
Sbjct: 878 DVVGTLRLRVTNETNKASTKMQASDMLRKAGIKDLTLQIE 901

BLAST of MELO3C009917 vs. TrEMBL
Match: W9RBJ6_9ROSA (Metal tolerance protein 12 OS=Morus notabilis GN=L484_010513 PE=4 SV=1)

HSP 1 Score: 32.7 bits (73), Expect = 2.3e+03
Identity = 10/14 (71.43%), Postives = 11/14 (78.57%), Query Frame = 1

Query: 510 HDHKHDHSHVHHHK 524
           HDH HDH H HHH+
Sbjct: 4   HDHDHDHHHHHHHR 17

HSP 2 Score: 30.4 bits (67), Expect = 1.1e+04
Identity = 9/13 (69.23%), Postives = 9/13 (69.23%), Query Frame = 1

Query: 522 HKHSHDHDHDHHH 535
           H H HDH H HHH
Sbjct: 4   HDHDHDHHHHHHH 16

HSP 3 Score: 969.5 bits (2505), Expect = 2.3e-279
Identity = 493/747 (66.00%), Postives = 585/747 (78.31%), Query Frame = 1

Query: 1   MSQQQLKTMVVKSVLLAIVFLLRFQALLYCGTAAMILAEMTGNVAARFLVEGRNQMTMGD 60
           ++Q Q + +V KS+LLA++FLLRFQAL YCGTAA+ILAE++GNVAARF+ EG+N+  +G 
Sbjct: 147 ITQSQYRLLVAKSLLLAVIFLLRFQALRYCGTAALILAELSGNVAARFIAEGKNRNFIG- 206

Query: 61  RTRSRSSEVRGFLSLFFGLFLLSISWDRIDCFPFATSFIDKYGFSVLPRENCMRIWPMLL 120
           +T+   S+VRGF++LF GLFLLSISWDR+DCFPF++  + K+G S  P +NC+RIWPMLL
Sbjct: 207 KTQIGMSKVRGFIALFSGLFLLSISWDRLDCFPFSSINVHKFGLSFFPGQNCIRIWPMLL 266

Query: 121 PFLSGFLGCYERISMNWGSVKQLGQKRVRLVSLFFTTIILFVPAVISMLLFEAEGKSVSL 180
           PFLSGFLGCYER+SMNWG+V+QLG KRVRL+SLFFTT++LFVPAVISM +FE EG S+S+
Sbjct: 267 PFLSGFLGCYERVSMNWGTVRQLGPKRVRLLSLFFTTVVLFVPAVISMFIFEVEGDSISI 326

Query: 181 GSLAWPLANTVVFGVLLNENYSDDKLVSSKDFRSEFLVTFVCTVILELLYFPELSLWGLL 240
            +L WPLANTVVFGVLL+ENY+D+K+ +SKDF+ E+LVTFVCTVILEL YFPELSLWGLL
Sbjct: 327 ANLGWPLANTVVFGVLLSENYTDEKVAASKDFQREYLVTFVCTVILELFYFPELSLWGLL 386

Query: 241 FCGLLLYVAVRELDPVFLNYHELGVESSDSIVTMVMRPVRHILTERKSRKIALFLLINTG 300
            CGLLL+V+V ELDPV  NY ELGVESSDS    +M+P+RHIL+ERKSRKIALFLLIN  
Sbjct: 387 ICGLLLWVSVSELDPVHPNYIELGVESSDSFAMSIMKPIRHILSERKSRKIALFLLINAA 446

Query: 301 YMVVEFVAGFMSNSLGLISDACHMLFDCAALAIGLYASYISRLPANNQFNYGRGRFEILS 360
           YMV+EFVAGFMSNSLGLISDACHMLFDCAAL IGLYASYISRLPAN QFNYGRGRFE+LS
Sbjct: 447 YMVIEFVAGFMSNSLGLISDACHMLFDCAALVIGLYASYISRLPANGQFNYGRGRFEVLS 506

Query: 361 GYANAVFLVLVGALIVLESLERILDPQEISTSSLLTVSIGGLVVNVVGLIFFHEEHHHAH 420
           GY NAVFLVLVGALIVLES ERILDPQEIST+SLL VS+GGL+VNVVGLIFFHEEHHHAH
Sbjct: 507 GYVNAVFLVLVGALIVLESFERILDPQEISTNSLLAVSVGGLLVNVVGLIFFHEEHHHAH 566

Query: 421 GGSGSCSHSHSHSHSQRNSHSCAGSHHHDKHEHDSCRKHENAISVTKECHESSVSVHTGH 480
           GGSG+C+HSHSHSH    S+S + SH H  H H S   H++   +  + HE    VH  H
Sbjct: 567 GGSGTCTHSHSHSH----SNSRSDSHSHHSHHHHS---HDHKCQIQHKTHECVPVVHNSH 626

Query: 481 HEHRQVDVCSENHLSNHCDDHHHLEHTDHHDHKHDHSHVHHHKHSHDHDHDHHHDCKHEH 540
                V  CS        D  HH+ H +HHD         +H+ S   + +     + + 
Sbjct: 627 -----VKPCSG-------DAEHHVGHHEHHD-------CDNHQKSDQKEGEECGTMQCDG 686

Query: 541 HDHVHRHDQHGHLSQPKSDVPKMVSHSVSESSHSHPSKQPVGG-----------TVRKKH 600
           HD +HR+D   H +        +  +  S  SH+  S   +G              RK+ 
Sbjct: 687 HDDLHRYD---HGAGTLICDGSLCVNGQSHGSHAGQSVHVLGDLHGHDHSHLEELNRKQQ 746

Query: 601 HHHHIDHNMEGIFLHVLADTMGSVGVVISTLLIKYKGWLVADPACSIFISIMIISSVIPL 660
           HHHHIDHNMEGIFLHVLADTMGSVGVV++TLLIKYKGWLVADPACSIFIS++IISSVIPL
Sbjct: 747 HHHHIDHNMEGIFLHVLADTMGSVGVVVATLLIKYKGWLVADPACSIFISVLIISSVIPL 806

Query: 661 LRNSAEILLQRVPRAHEQDLKEAVSDIMEIHGVQGIQNLHVWSFTNTDVVGTLRLHVSTE 720
           LRNSAEILLQRVPRA EQDL++AV+D M++ GV+GIQNLH WS TNTDVVGTL LH+STE
Sbjct: 807 LRNSAEILLQRVPRALEQDLRQAVNDAMKVDGVRGIQNLHFWSLTNTDVVGTLHLHISTE 863

Query: 721 TDKPSIKAKVEHILHDAGIKDLTLQLE 737
             K S KA+V H+L DAGIKDLTLQ+E
Sbjct: 867 ASKASAKAQVSHVLEDAGIKDLTLQVE 863

BLAST of MELO3C009917 vs. TrEMBL
Match: V4W1E4_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10014254mg PE=4 SV=1)

HSP 1 Score: 968.4 bits (2502), Expect = 5.0e-279
Identity = 491/735 (66.80%), Postives = 576/735 (78.37%), Query Frame = 1

Query: 7   KTMVVKSVLLAIVFLLRFQALLYCGTAAMILAEMTGNVAARFLVEGRNQMTMGDRTRSRS 66
           K+++ KS LLA VFLLRFQAL YCGTAAMILAE++GNVA RF+ EGR   +  D   S+ 
Sbjct: 135 KSLLAKSFLLACVFLLRFQALRYCGTAAMILAEISGNVAGRFVAEGRKNGSFTDW--SKF 194

Query: 67  SEVRGFLSLFFGLFLLSISWDRIDCFPFATSFIDKYGFSVLPRENCMRIWPMLLPFLSGF 126
           S+V GF  +F GLFLLS+SWDR +CFP ++SF+ K+    L  E+C+R+WPMLLPFLSGF
Sbjct: 195 SKVGGFSCMFVGLFLLSVSWDRTECFPLSSSFLGKWS---LYGEHCVRLWPMLLPFLSGF 254

Query: 127 LGCYERISMNWGSVKQLGQKRVRLVSLFFTTIILFVPAVISMLLFEAEG----KSVSLGS 186
           LGCYER+SMNWG+++QL QK+VRL+SLF+TT++L VPA++S ++FE  G     S+S  +
Sbjct: 255 LGCYERVSMNWGAIRQLSQKQVRLISLFYTTVLLSVPALVSWIVFENYGDGDESSISFAN 314

Query: 187 LAWPLANTVVFGVLLNENYSDDKLVSSKDFRSEFLVTFVCTVILELLYFPELSLWGLLFC 246
           LAWPL NTVVFGVLL+ENYSD K V S DF+ EF+VT VCT++LEL Y+PELSLWGLL C
Sbjct: 315 LAWPLGNTVVFGVLLSENYSDQKFVISADFQREFVVTIVCTIVLELFYYPELSLWGLLLC 374

Query: 247 GLLLYVAVRELDPVFLNYHELGVESSDSIVTMVMRPVRHILTERKSRKIALFLLINTGYM 306
            LLLY AVRELDPV+ NYHELG ESS+S  +++M+P+RHIL+ERKSRKIALFLLINTGYM
Sbjct: 375 VLLLYFAVRELDPVYSNYHELGFESSESFSSLIMKPIRHILSERKSRKIALFLLINTGYM 434

Query: 307 VVEFVAGFMSNSLGLISDACHMLFDCAALAIGLYASYISRLPANNQFNYGRGRFEILSGY 366
           VVEFVAGFMSNSLGLISDACHMLFDCAALAIGLYASYISRLPAN+QFNYGRGRFE+LSGY
Sbjct: 435 VVEFVAGFMSNSLGLISDACHMLFDCAALAIGLYASYISRLPANSQFNYGRGRFEVLSGY 494

Query: 367 ANAVFLVLVGALIVLESLERILDPQEISTSSLLTVSIGGLVVNVVGLIFFHEEHHHAHGG 426
            NAVFLVLVGALIVLES ERILDPQEIST+SLLTVSIGGL+VNVVGLIFFHEEHHHAHG 
Sbjct: 495 TNAVFLVLVGALIVLESFERILDPQEISTNSLLTVSIGGLLVNVVGLIFFHEEHHHAHG- 554

Query: 427 SGSCSHSHSHSHSQRNSHSCAGSHHHDKHEHDSCRKHENAISVTKECHESSVSVHTGHHE 486
            G CSHSHSHSHS  +       HH   H+H+   KH+  IS++ E +E S S H   H 
Sbjct: 555 -GVCSHSHSHSHSHPH------HHHQHSHDHEGHGKHQECISISHESNEKSCSSH--DHR 614

Query: 487 HRQVDVCSENHLSNHCDDHHHLEHTDHHDHKHDHSHVHHHKHSHDH-DHDHHHDCKHEHH 546
           H        +   +HCD     EHT  HD +       HH H+H H +H HHH   H   
Sbjct: 615 HCTGHTAHHHGRCDHCDSTLKHEHTHGHDDQGLGDQHSHHDHTHKHNNHYHHHPANHNF- 674

Query: 547 DHVHRHDQHGHLSQPKSDVPKMVSHSVSESSHSHPSKQPVGGTVRKKHHHHHIDHNMEGI 606
            H H HD H H       V + + HS S+S H+H +++P+      KH   HIDHNMEGI
Sbjct: 675 -HAHEHDDHDHHHHADHHVNQSLLHSASKSLHAHSNEEPL------KHDRRHIDHNMEGI 734

Query: 607 FLHVLADTMGSVGVVISTLLIKYKGWLVADPACSIFISIMIISSVIPLLRNSAEILLQRV 666
           FLHVLADTMGSVGVVISTLLIKYKGWLVADPACSIFIS++I+SSVIPLLRNSAEILLQRV
Sbjct: 735 FLHVLADTMGSVGVVISTLLIKYKGWLVADPACSIFISLLIVSSVIPLLRNSAEILLQRV 794

Query: 667 PRAHEQDLKEAVSDIMEIHGVQGIQNLHVWSFTNTDVVGTLRLHVSTETDKPSIKAKVEH 726
            RAHE DLKE ++D+M+I GV GIQNLH+WSFT+TDVVGTL LHVS+E DK SIKA+V H
Sbjct: 795 SRAHELDLKETLNDVMKISGVHGIQNLHLWSFTSTDVVGTLNLHVSSEADKASIKAQVSH 846

Query: 727 ILHDAGIKDLTLQLE 737
           +L DAGIKDLTLQ+E
Sbjct: 855 MLSDAGIKDLTLQVE 846

BLAST of MELO3C009917 vs. TrEMBL
Match: A0A061ELT5_THECC (Cation efflux family protein OS=Theobroma cacao GN=TCM_017990 PE=4 SV=1)

HSP 1 Score: 967.6 bits (2500), Expect = 8.6e-279
Identity = 503/763 (65.92%), Postives = 585/763 (76.67%), Query Frame = 1

Query: 1   MSQQQLKTMVVKSVLLAIVFLLRFQALLYCGTAAMILAEMTGNVAARFLVEGRNQMTMGD 60
           +S+ QLK ++ KS LL+I+FLLRFQAL YCGTAAMILAE++GN+A R         T   
Sbjct: 139 LSKTQLKLLLSKSFLLSIIFLLRFQALRYCGTAAMILAELSGNLAGRLF-----SRTDTP 198

Query: 61  RTRSR-SSEVRGFLSLFFGLFLLSISWDRIDCFPFATSFIDKYGFSVLPRENCMRIWPML 120
           + RS  SS   GF  LF GL LLS+SWDR+DCFP     ++K GFS+ PRE C+R+WPML
Sbjct: 199 KIRSFFSSRFIGFCFLFIGLMLLSLSWDRVDCFP-----LNKSGFSIYPREGCVRVWPML 258

Query: 121 LPFLSGFLGCYERISMNWGSVKQLGQKRVRLVSLFFTTIILFVPAVISMLLFEAEGK--- 180
           LPFLSG LGCYE++SMNWGS++QLG+KRVRL+SLFF+T++LFVPAV+S  ++E+EG    
Sbjct: 259 LPFLSGLLGCYEKVSMNWGSIRQLGRKRVRLISLFFSTVMLFVPAVVSFFVYESEGGGGG 318

Query: 181 SVSLGSLAWPLANTVVFGVLLNENYSDD--KLVSSKDFRSEFLVTFVCTVILELLYFPEL 240
           +VS+ +L WPL NTVVFGVLL+ENYSDD  KLV+ KDF+ EF+VTF CTV+LEL YF EL
Sbjct: 319 NVSVENLVWPLVNTVVFGVLLSENYSDDHHKLVNPKDFQREFVVTFACTVVLELFYFDEL 378

Query: 241 SLWGLLFCGLLLYVAVRELDPVFLNYHELGVESSDSIVTMVMRPVRHILTERKSRKIALF 300
           SL GLL CGLLLY AVRELDP + +Y ELG+ESS+S    +M+P+RHIL+ERKSRKIALF
Sbjct: 379 SLLGLLLCGLLLYFAVRELDPDYWSYLELGMESSESFSMSIMKPIRHILSERKSRKIALF 438

Query: 301 LLINTGYMVVEFVAGFMSNSLGLISDACHMLFDCAALAIGLYASYISRLPANNQFNYGRG 360
           LLINTGYMVVEFVAGFMSNSLGLISDACHMLFDCAALAIGLYASYISRLPANNQFNYGRG
Sbjct: 439 LLINTGYMVVEFVAGFMSNSLGLISDACHMLFDCAALAIGLYASYISRLPANNQFNYGRG 498

Query: 361 RFEILSGYANAVFLVLVGALIVLESLERILDPQEISTSSLLTVSIGGLVVNVVGLIFFHE 420
           RFEILSGYANAVFLVLVGALIVLES ERILDPQEIST+SLLTVSIGGLVVNVVGLIFFHE
Sbjct: 499 RFEILSGYANAVFLVLVGALIVLESFERILDPQEISTNSLLTVSIGGLVVNVVGLIFFHE 558

Query: 421 EHHHAHGGSGSCSHSHSHSHSQRNSHSCAGSHHHDKHEHDSCRKHENAISVTKECHESSV 480
           EHHHAHG  GSCSHSHSHSH   N H     HH   H+H+S  +H N I+V+  C  S  
Sbjct: 559 EHHHAHG--GSCSHSHSHSHFYSNDH-----HHQHSHDHESHVEHHNFINVSDGCQGS-- 618

Query: 481 SVHTGHHEHRQVDVCSENHLSNHCDDHH-HLEHTDHHDHKHDHS------HVHHHKHSHD 540
                 HEH  ++    N+   H +DH  H E  DHHDH HD +      H  HH H H 
Sbjct: 619 ---CSGHEHNHINQHGSNNC--HAEDHDIHTECHDHHDHAHDQAYHDCAYHHDHHDHGHQ 678

Query: 541 HDHDHHHDCK--HEHHDHVHRHDQ-----------HGHLSQPKSDVPKMVSHSVSESSH- 600
           HDH H HDC   H+HHDH H++D            H H    +S +   +++S S+ S  
Sbjct: 679 HDHAHSHDCAHYHDHHDHRHQYDHKDNHHEHGAGLHSHTHYSESKIHLSLAYSGSKQSQL 738

Query: 601 SHPSKQPVGGTVRKKHHHHHIDHNMEGIFLHVLADTMGSVGVVISTLLIKYKGWLVADPA 660
               K+P      +KH HHHIDHNMEGIFLHVLADTMGSVGVV+STLLIKYKGWLVADPA
Sbjct: 739 LSEGKEP------QKHQHHHIDHNMEGIFLHVLADTMGSVGVVVSTLLIKYKGWLVADPA 798

Query: 661 CSIFISIMIISSVIPLLRNSAEILLQRVPRAHEQDLKEAVSDIMEIHGVQGIQNLHVWSF 720
           CSIFIS++IISSV PLLRNSA+ILLQRVPRA EQ LK+A+SD+M+  GV  IQNLHVWSF
Sbjct: 799 CSIFISVLIISSVFPLLRNSADILLQRVPRAQEQGLKKAISDVMKTKGVIRIQNLHVWSF 858

Query: 721 TNTDVVGTLRLHVSTETDKPSIKAKVEHILHDAGIKDLTLQLE 737
           T  D+VGT+ LH+S ETDK + KA+V HILHDAGIKDLTLQ+E
Sbjct: 859 TTMDIVGTIHLHISAETDKAATKAQVSHILHDAGIKDLTLQVE 871

BLAST of MELO3C009917 vs. TrEMBL
Match: A0A059AF92_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_J01747 PE=4 SV=1)

HSP 1 Score: 955.7 bits (2469), Expect = 3.4e-275
Identity = 498/752 (66.22%), Postives = 577/752 (76.73%), Query Frame = 1

Query: 1   MSQQQLKTMVVKSVLLAIVFLLRFQALLYCGTAAMILAEMTGNVAARFLVEGRNQMTMGD 60
           +S  Q K +  KSVLLA+VFLLRFQAL YCG AAMILAE++GNVAARFL EGR     G 
Sbjct: 147 LSPSQNKVLAEKSVLLAVVFLLRFQALRYCGAAAMILAELSGNVAARFLAEGRI-WDSGY 206

Query: 61  RTRSRSSEVRGFLSLFFGLFLLSISWDRIDCFPFA--TSFIDKYGFSVLPRENCMRIWPM 120
             ++RSS VRGF +LF GLFLL++SWDR  CFPF+  +S  DK+ F VLPRENC+RIWPM
Sbjct: 207 LGKNRSSRVRGFAALFLGLFLLALSWDRNMCFPFSGSSSSDDKWRFLVLPRENCLRIWPM 266

Query: 121 LLPFLSGFLGCYERISMNWGSVKQLGQKRVRLVSLFFTTIILFVPAVISMLLFEAEGKSV 180
           LLPFLSGFLGCYER+SMN G +++LGQKRVRL+SL +T+I+L VPAVIS+L+FE EG S+
Sbjct: 267 LLPFLSGFLGCYERVSMNRGVLQELGQKRVRLISLLYTSIVLLVPAVISILVFEHEGDSI 326

Query: 181 SLGSLAWPLANTVVFGVLLNENYSDDKLVSSKDFRSEFLVTFVCTVILELLYFPELSLWG 240
           S+ SL WPLANTVVFGVLL+EN SD+K VSSKDFR EFLVTF CTV+LEL YFPELSLWG
Sbjct: 327 SVTSLGWPLANTVVFGVLLSENCSDEKFVSSKDFRREFLVTFACTVVLELFYFPELSLWG 386

Query: 241 LLFCGLLLYVAVRELDPVFLNYHELGVESSDSIVTMVMRPVRHILTERKSRKIALFLLIN 300
           LL CG+LLYVAV ELDP++ +Y ELG+ESS+   T +++P+RHIL+ERKSRKIALFLLIN
Sbjct: 387 LLLCGILLYVAVAELDPIYSSYAELGLESSEPFTTSIIKPIRHILSERKSRKIALFLLIN 446

Query: 301 TGYMVVEFVAGFMSNSLGLISDACHMLFDCAALAIGLYASYISRLPANNQFNYGRGRFEI 360
            GYMVVEFV GFMSNSLGLISDACHMLFDCAALAIGLYASYISRLPAN QFNYGRGRFE+
Sbjct: 447 AGYMVVEFVVGFMSNSLGLISDACHMLFDCAALAIGLYASYISRLPANGQFNYGRGRFEV 506

Query: 361 LSGYANAVFLVLVGALIVLESLERILDPQEISTSSLLTVSIGGLVVNVVGLIFFHEEHHH 420
           LSGY NAVFLVLVGALIVLES+ERILDPQEIST+SLL VSIGGL+VNV+GLIFFHEEHHH
Sbjct: 507 LSGYVNAVFLVLVGALIVLESIERILDPQEISTNSLLIVSIGGLLVNVIGLIFFHEEHHH 566

Query: 421 AHGGSGSCSHSHSHSHSQRNSHSCAGSHHHDKHEHDSCRKHENAISVTKECHESSVSVHT 480
           AHG  GSCSHS SHS S  +SHS    H HD   HD   + +  ISV  +CHE S S H 
Sbjct: 567 AHG--GSCSHSPSHSGSHSHSHS---QHQHD---HDCHIEDQKCISVEHDCHEKSCSGHH 626

Query: 481 GHHEHRQVD--------VCSENHLSNHCDDHHHLEHTDHHDHKHDHSHVHHHKHSHDHDH 540
             H+H   D        V      S+ C  H H +  +H +  H   H HH+ H+H H H
Sbjct: 627 NQHDHHHSDQHGNGDAKVAYPTLGSSSCHGHDHAD--NHSEGSHGCDHAHHNDHAHGHGH 686

Query: 541 DHHHDCKHEHHDHV--HRHDQHGHLS---QPKSDVPKMVSHSVSES-SHSHPSKQPVGGT 600
           DHH      HH+ V  H H   GH       ++  P   +H +S S S  H    P    
Sbjct: 687 DHH------HHEPVFTHHHSTEGHADGCLPTQTAAPH--AHGLSSSISQIHQRNLPSSEG 746

Query: 601 VRKKHHHHHIDHNMEGIFLHVLADTMGSVGVVISTLLIKYKGWLVADPACSIFISIMIIS 660
             KKH HHHIDHNMEGIFLHVLADTMGSVGVV+STLLIKYKGWLVADPA SIFISI+I+ 
Sbjct: 747 EAKKHSHHHIDHNMEGIFLHVLADTMGSVGVVVSTLLIKYKGWLVADPASSIFISILIVL 806

Query: 661 SVIPLLRNSAEILLQRVPRAHEQDLKEAVSDIMEIHGVQGIQNLHVWSFTNTDVVGTLRL 720
           SVIPLLRNSAE+LLQRVPRAHE+DL  A++++ +I  V+GI + H WSFTNT+VVGT+ L
Sbjct: 807 SVIPLLRNSAEVLLQRVPRAHERDLFNALNNVTKIKQVRGIPHFHAWSFTNTEVVGTIHL 866

Query: 721 HVSTETDKPSIKAKVEHILHDAGIKDLTLQLE 737
           HV TE+DK S KA+V  +LHD GI DLTLQ+E
Sbjct: 867 HVETESDKASAKAQVSRMLHDVGINDLTLQVE 879

BLAST of MELO3C009917 vs. TAIR10
Match: AT2G04620.1 (AT2G04620.1 Cation efflux family protein)

HSP 1 Score: 769.6 bits (1986), Expect = 1.7e-222
Identity = 420/749 (56.07%), Postives = 511/749 (68.22%), Query Frame = 1

Query: 1   MSQQQLKTMVVKSVLLAIVFLLRFQALLYCGTAAMILAEMTGNVAARFLVEGRNQMTMGD 60
           +S  Q+K ++ KS LLA VFLLRFQAL YCG AAMILAE++G V+AR L      + +  
Sbjct: 135 LSSSQIKLLLAKSFLLAFVFLLRFQALRYCGAAAMILAELSGTVSARVLFSDTGGIGV-- 194

Query: 61  RTRSRSSEVRGFLSLFFGLFLLSISWDRIDCFPFATSFIDKYGFSVLPRENCMRIWPMLL 120
               RSS+VRGF  LF GL LLSISWDR+DCFPF++S ++ +GF + P+ENC+RIWP+LL
Sbjct: 195 ----RSSKVRGFCVLFAGLLLLSISWDRVDCFPFSSS-VESWGFWIYPKENCLRIWPLLL 254

Query: 121 PFLSGFLGCYERISMNWGSVKQLGQKRVRLVSLFFTTIILFVPAVISMLLFEAEGKSVSL 180
           PFLSGFLGCYE++S+NW  +KQL QKRVRL+SLF TT++LF  A+ S     +   SVS 
Sbjct: 255 PFLSGFLGCYEKVSVNWNEIKQLDQKRVRLLSLFLTTVLLFPLAIWSFFFSGSGDDSVSF 314

Query: 181 GSLAWPLANTVVFGVLLNENYSDDKLVSSK--DFRSEFLVTFVCTVILELLYFPELSLWG 240
           G+L WPLANTVVFGVLL+ENY+DDK  SSK  D   EFLVTF+CT++LEL YFPELSLWG
Sbjct: 315 GNLGWPLANTVVFGVLLSENYNDDKFSSSKKKDSEREFLVTFLCTIVLELFYFPELSLWG 374

Query: 241 LLFCGLLLYVAVRELDPVFLNYHELGVESSDSIVTMVMRPVRHILTERKSRKIALFLLIN 300
           LL CGLLLY+AVREL+ V+ +Y E+G+ES +S  TM M+P+RHIL+E+KSRKIALFLLIN
Sbjct: 375 LLLCGLLLYIAVRELESVYSDYQEIGMESPESFSTMFMKPIRHILSEKKSRKIALFLLIN 434

Query: 301 TGYMVVEFVAGFMSNSLGLISDACHMLFDCAALAIGLYASYISRLPANNQFNYGRGRFEI 360
           T YMVVEFVAGFMSNSLGLISDACHMLFDCAALAIGLYASYISRLPAN+Q+NYGRGRFE+
Sbjct: 435 TAYMVVEFVAGFMSNSLGLISDACHMLFDCAALAIGLYASYISRLPANHQYNYGRGRFEV 494

Query: 361 LSGYANAVFLVLVGALIVLESLERILDPQEISTSSLLTVSIGGLVVNVVGLIFFHEEHHH 420
           LSGY NAVFLVLVGALIVLES+ERILDPQEIST+SLL VS+GGL+VN+VGLIFFHEEHHH
Sbjct: 495 LSGYVNAVFLVLVGALIVLESIERILDPQEISTNSLLVVSVGGLLVNIVGLIFFHEEHHH 554

Query: 421 AHGGSGSCSHSHSH-SHSQRNS--HSCAGSHHHDK-HEHDSCRKHENAISVTKECHESSV 480
           AHGGSG C+HSHSH SHS +N   H  + SH H++ H+H    KHE              
Sbjct: 555 AHGGSG-CTHSHSHQSHSHKNEEHHQHSDSHKHEEHHQHSDSHKHEE------------- 614

Query: 481 SVHTGHHEHRQVDVCSENHLSNHCDDHHHLEHTDHHD---HKHDHSHVHHHKHSHD---H 540
                HHEH                DHHH  H+  H+   H HDH H   H H+H+   H
Sbjct: 615 -----HHEH----------------DHHHHSHSHKHEECNHNHDHEH-QSHSHNHEECNH 674

Query: 541 DHDHHHDCKHEHHD-HVHRHDQHGHLSQPKSDVPKMVSHSVSESSHSHPSKQPVGGTVRK 600
           +HDHH D + E  +   HRH  H        ++  +  H ++++     S   V  T+  
Sbjct: 675 NHDHHSDHQPEKSEKKEHRHIDH--------NMEGIFLHVLADTM---GSVGVVISTLLI 734

Query: 601 KHHHHHIDHNMEGIFLHVLADTMGSVGVVISTLLIKYKGWLVADPACSIFISIMIISSVI 660
           K+    +      IF+ +L                             I  S++ +    
Sbjct: 735 KYKGWLVADPASSIFISIL-----------------------------IIASVIPL---- 794

Query: 661 PLLRNSAEILLQRVPRAHEQDLKEAVSDIMEIHGVQGIQNLHVWSFTNTDVVGTLRLHVS 720
             LRNSAEILLQRVPRAH QDLKEA+ +I++  GV  IQ LHVWSFTN+DVV TL L VS
Sbjct: 795 --LRNSAEILLQRVPRAHRQDLKEAMRNILKTKGVCSIQRLHVWSFTNSDVVATLHLLVS 794

Query: 721 TETDKPSIKAKVEHILHDAGIKDLTLQLE 737
            ++DK   K +V  +L DAG+KD TLQ+E
Sbjct: 855 ADSDKTDTKLQVSRLLEDAGVKDWTLQVE 794

BLAST of MELO3C009917 vs. TAIR10
Match: AT3G58810.1 (AT3G58810.1 metal tolerance protein A2)

HSP 1 Score: 108.6 bits (270), Expect = 1.7e-23
Identity = 62/181 (34.25%), Postives = 93/181 (51.38%), Query Frame = 1

Query: 289 RKIALFLLINTGYMVVEFVAGFMSNSLGLISDACHMLFDCAALAIGLYASYISRLPANNQ 348
           RK+ + +L+   ++VVE V G  +NSL +++DA H+L D AA AI L++ + S   AN Q
Sbjct: 111 RKLLIAVLLCAIFIVVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWKANPQ 170

Query: 349 FNYGRGRFEILSGYANAVFLVLVGALIVLESLERILDPQ-EISTSSLLTVSIGGLVVNVV 408
            +YG  R EIL    +   + L+  ++V E++ R+ +   E+  S +  VS  GL+VN+ 
Sbjct: 171 QSYGFFRIEILGALVSIQMIWLLAGILVYEAIVRLNNGSGEVEGSLMFAVSAVGLLVNIA 230

Query: 409 GLIFFHEEHHHAHGGSGSCSHSHSHSHSQRNSHSCAGSHHHDKHEHDSCRKHENAISVTK 468
             I    +H H HG S    H HSH H     H  A + HH    HD  +  +  I   K
Sbjct: 231 MAILLGHDHGHGHGHSHDNGHGHSHDH----GHGIAATEHHHDSGHDESQLSDVLIEQKK 287

BLAST of MELO3C009917 vs. TAIR10
Match: AT2G46800.1 (AT2G46800.1 zinc transporter of Arabidopsis thaliana)

HSP 1 Score: 100.9 bits (250), Expect = 3.5e-21
Identity = 55/173 (31.79%), Postives = 90/173 (52.02%), Query Frame = 1

Query: 289 RKIALFLLINTGYMVVEFVAGFMSNSLGLISDACHMLFDCAALAIGLYASYISRLPANNQ 348
           RK+ + +++   +M VE V G  +NSL +++DA H+L D AA AI L++ + +   A  +
Sbjct: 56  RKLCIAVVLCLVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWAAGWEATPR 115

Query: 349 FNYGRGRFEILSGYANAVFLVLVGALIVLESLERIL-DPQEISTSSLLTVSIGGLVVNVV 408
             YG  R EIL    +   + L+  ++V E++ RI+ +  E++   +  V+  GLVVN++
Sbjct: 116 QTYGFFRIEILGALVSIQLIWLLTGILVYEAIIRIVTETSEVNGFLMFLVAAFGLVVNII 175

Query: 409 GLIFFHEEHHHAHGGSGSCSHSHSHSHSQRN-SHSCAGSHHHDKHEHDSCRKH 460
             +    +H H+HG      H H H H   N SH    + HH  H+H+    H
Sbjct: 176 MAVLLGHDHGHSHG------HGHGHGHDHHNHSHGVTVTTHHHHHDHEHGHSH 222

BLAST of MELO3C009917 vs. TAIR10
Match: AT2G29410.1 (AT2G29410.1 metal tolerance protein B1)

HSP 1 Score: 94.4 bits (233), Expect = 3.2e-19
Identity = 64/227 (28.19%), Postives = 103/227 (45.37%), Query Frame = 1

Query: 514 HDHSHVHHHKHSHDHDHDHHHDCKHEHHDHVHRHDQHGHLSQPKSDVPKMVSHSVSESSH 573
           H+HSH             HHHD  H HH+H H+H  H               H    +  
Sbjct: 182 HNHSH-------------HHHDHHHHHHNHKHQHQHH---------------HKEVVAEE 241

Query: 574 SHPSKQPVGGTVRKKHHHHHIDHNMEGIFLHVLADTMGSVGVVISTLLIKYK-GWLVADP 633
                 P+ G   +K     ++ N++G +LH +AD + S+GV+I   +I  K  W++ D 
Sbjct: 242 EEEEMNPLKG---EKSSSKEMNINIQGAYLHAMADMIQSLGVMIGGGIIWVKPKWVLVDL 301

Query: 634 ACSIFISIMIISSVIPLLRNSAEILLQRVPRAHEQDLKEAVSDIMEIHGVQGIQNLHVWS 693
            C++  S   +++ +P+L+N   IL++RVPR  + D+++    +  I GV+ + +LHVW 
Sbjct: 302 ICTLVFSAFALAATLPILKNIFGILMERVPR--DMDIEKLERGLKRIDGVKIVYDLHVWE 361

Query: 694 FTNTDVVGTLRLHVSTE---TDKPSIKAKVEHILHDAGIKDLTLQLE 737
            T   +V  L  H+  E   + K  I           GI   T+Q+E
Sbjct: 362 ITVGRIV--LSCHILPEPGASPKEIITGVRNFCRKSYGIYHATVQVE 373

HSP 2 Score: 45.4 bits (106), Expect = 1.7e-04
Identity = 14/25 (56.00%), Postives = 15/25 (60.00%), Query Frame = 1

Query: 510 HDHKHDHSHVHHHKHSHDHDHDHHH 535
           H+H H H   HHH H+H H H HHH
Sbjct: 182 HNHSHHHHDHHHHHHNHKHQHQHHH 206

BLAST of MELO3C009917 vs. TAIR10
Match: AT3G61940.1 (AT3G61940.1 Cation efflux family protein)

HSP 1 Score: 90.5 bits (223), Expect = 4.7e-18
Identity = 59/198 (29.80%), Postives = 97/198 (48.99%), Query Frame = 1

Query: 541 HDHVHRHDQHGHLSQPKSDVPKMVSHSVSESSHSHPSKQPVGGTVRKKHHHHHIDHNMEG 600
           HDH H HD HGH            SH      H H   +     + K     +I  N++G
Sbjct: 158 HDHGHGHD-HGH------------SHD-----HGHSYGERAEQLLEKSKEIRNI--NVQG 217

Query: 601 IFLHVLADTMGSVGVVISTLLIKYKG-WLVADPACSIFISIMIISSVIPLLRNSAEILLQ 660
            +LHVL D + S+GV+I   +I Y   W V D  C++F S++++ + I +LR+  E+L++
Sbjct: 218 AYLHVLGDLIQSIGVMIGGGMIWYNPKWKVIDLICTLFFSVIVLGTTIKMLRSILEVLME 277

Query: 661 RVPRAHEQDLKEAVSDIMEIHGVQGIQNLHVWSFTNTDVVGTLRLHVSTET-DKPSIKAK 720
             PR  E D ++    +MEI  V  +  LH+W+ T    + +  + V  E  D+  +   
Sbjct: 278 STPR--EIDARQLEKGLMEIEEVVDVHELHIWAITVGKALFSCHVKVRPEAGDEMVLNKV 333

Query: 721 VEHILHDAGIKDLTLQLE 737
           +++I  +  I  +T+Q+E
Sbjct: 338 IDYIWREYRISHVTIQIE 333

HSP 2 Score: 84.0 bits (206), Expect = 4.4e-16
Identity = 46/151 (30.46%), Postives = 80/151 (52.98%), Query Frame = 1

Query: 289 RKIALFLLINTGYMVVEFVAGFMSNSLGLISDACHMLFDCAALAIGLYASYISRLPANNQ 348
           RK+   +++   +M +E V G  +NSL +++DA H+L D  A AI + + + S   AN +
Sbjct: 31  RKLCFVVVLCLLFMSIEVVCGIKANSLAILADAAHLLTDVGAFAISMLSLWASSWEANPR 90

Query: 349 FNYGRGRFEILSGYANAVFLVLVGALIVLESLERILDP--QEISTSSLLTVSIGGLVVNV 408
            +YG  R EIL    +   + L+  ++V E++ R++     ++    ++ V+  GLVVN+
Sbjct: 91  QSYGFFRIEILGTLVSIQLIWLLTGILVYEAVTRLVQETNDDVDGFFMVLVAAFGLVVNI 150

Query: 409 VGLIFFHEEHHHAHGGSGSCSHSHSHSHSQR 438
           + ++     H H HG     SH H HS+ +R
Sbjct: 151 IMIVVL--GHDHGHGHDHGHSHDHGHSYGER 179

HSP 3 Score: 36.6 bits (83), Expect = 8.0e-02
Identity = 12/19 (63.16%), Postives = 11/19 (57.89%), Query Frame = 1

Query: 514 HDHSHVHHHKHSHDHDHDH 533
           HDH H H H HSHDH H +
Sbjct: 158 HDHGHGHDHGHSHDHGHSY 176

BLAST of MELO3C009917 vs. NCBI nr
Match: gi|659085522|ref|XP_008443464.1| (PREDICTED: probable zinc transporter protein DDB_G0291141 [Cucumis melo])

HSP 1 Score: 1516.5 bits (3925), Expect = 0.0e+00
Identity = 742/742 (100.00%), Postives = 742/742 (100.00%), Query Frame = 1

Query: 1   MSQQQLKTMVVKSVLLAIVFLLRFQALLYCGTAAMILAEMTGNVAARFLVEGRNQMTMGD 60
           MSQQQLKTMVVKSVLLAIVFLLRFQALLYCGTAAMILAEMTGNVAARFLVEGRNQMTMGD
Sbjct: 140 MSQQQLKTMVVKSVLLAIVFLLRFQALLYCGTAAMILAEMTGNVAARFLVEGRNQMTMGD 199

Query: 61  RTRSRSSEVRGFLSLFFGLFLLSISWDRIDCFPFATSFIDKYGFSVLPRENCMRIWPMLL 120
           RTRSRSSEVRGFLSLFFGLFLLSISWDRIDCFPFATSFIDKYGFSVLPRENCMRIWPMLL
Sbjct: 200 RTRSRSSEVRGFLSLFFGLFLLSISWDRIDCFPFATSFIDKYGFSVLPRENCMRIWPMLL 259

Query: 121 PFLSGFLGCYERISMNWGSVKQLGQKRVRLVSLFFTTIILFVPAVISMLLFEAEGKSVSL 180
           PFLSGFLGCYERISMNWGSVKQLGQKRVRLVSLFFTTIILFVPAVISMLLFEAEGKSVSL
Sbjct: 260 PFLSGFLGCYERISMNWGSVKQLGQKRVRLVSLFFTTIILFVPAVISMLLFEAEGKSVSL 319

Query: 181 GSLAWPLANTVVFGVLLNENYSDDKLVSSKDFRSEFLVTFVCTVILELLYFPELSLWGLL 240
           GSLAWPLANTVVFGVLLNENYSDDKLVSSKDFRSEFLVTFVCTVILELLYFPELSLWGLL
Sbjct: 320 GSLAWPLANTVVFGVLLNENYSDDKLVSSKDFRSEFLVTFVCTVILELLYFPELSLWGLL 379

Query: 241 FCGLLLYVAVRELDPVFLNYHELGVESSDSIVTMVMRPVRHILTERKSRKIALFLLINTG 300
           FCGLLLYVAVRELDPVFLNYHELGVESSDSIVTMVMRPVRHILTERKSRKIALFLLINTG
Sbjct: 380 FCGLLLYVAVRELDPVFLNYHELGVESSDSIVTMVMRPVRHILTERKSRKIALFLLINTG 439

Query: 301 YMVVEFVAGFMSNSLGLISDACHMLFDCAALAIGLYASYISRLPANNQFNYGRGRFEILS 360
           YMVVEFVAGFMSNSLGLISDACHMLFDCAALAIGLYASYISRLPANNQFNYGRGRFEILS
Sbjct: 440 YMVVEFVAGFMSNSLGLISDACHMLFDCAALAIGLYASYISRLPANNQFNYGRGRFEILS 499

Query: 361 GYANAVFLVLVGALIVLESLERILDPQEISTSSLLTVSIGGLVVNVVGLIFFHEEHHHAH 420
           GYANAVFLVLVGALIVLESLERILDPQEISTSSLLTVSIGGLVVNVVGLIFFHEEHHHAH
Sbjct: 500 GYANAVFLVLVGALIVLESLERILDPQEISTSSLLTVSIGGLVVNVVGLIFFHEEHHHAH 559

Query: 421 GGSGSCSHSHSHSHSQRNSHSCAGSHHHDKHEHDSCRKHENAISVTKECHESSVSVHTGH 480
           GGSGSCSHSHSHSHSQRNSHSCAGSHHHDKHEHDSCRKHENAISVTKECHESSVSVHTGH
Sbjct: 560 GGSGSCSHSHSHSHSQRNSHSCAGSHHHDKHEHDSCRKHENAISVTKECHESSVSVHTGH 619

Query: 481 HEHRQVDVCSENHLSNHCDDHHHLEHTDHHDHKHDHSHVHHHKHSHDHDHDHHHDCKHEH 540
           HEHRQVDVCSENHLSNHCDDHHHLEHTDHHDHKHDHSHVHHHKHSHDHDHDHHHDCKHEH
Sbjct: 620 HEHRQVDVCSENHLSNHCDDHHHLEHTDHHDHKHDHSHVHHHKHSHDHDHDHHHDCKHEH 679

Query: 541 HDHVHRHDQHGHLSQPKSDVPKMVSHSVSESSHSHPSKQPVGGTVRKKHHHHHIDHNMEG 600
           HDHVHRHDQHGHLSQPKSDVPKMVSHSVSESSHSHPSKQPVGGTVRKKHHHHHIDHNMEG
Sbjct: 680 HDHVHRHDQHGHLSQPKSDVPKMVSHSVSESSHSHPSKQPVGGTVRKKHHHHHIDHNMEG 739

Query: 601 IFLHVLADTMGSVGVVISTLLIKYKGWLVADPACSIFISIMIISSVIPLLRNSAEILLQR 660
           IFLHVLADTMGSVGVVISTLLIKYKGWLVADPACSIFISIMIISSVIPLLRNSAEILLQR
Sbjct: 740 IFLHVLADTMGSVGVVISTLLIKYKGWLVADPACSIFISIMIISSVIPLLRNSAEILLQR 799

Query: 661 VPRAHEQDLKEAVSDIMEIHGVQGIQNLHVWSFTNTDVVGTLRLHVSTETDKPSIKAKVE 720
           VPRAHEQDLKEAVSDIMEIHGVQGIQNLHVWSFTNTDVVGTLRLHVSTETDKPSIKAKVE
Sbjct: 800 VPRAHEQDLKEAVSDIMEIHGVQGIQNLHVWSFTNTDVVGTLRLHVSTETDKPSIKAKVE 859

Query: 721 HILHDAGIKDLTLQLEHNRELS 743
           HILHDAGIKDLTLQLEHNRELS
Sbjct: 860 HILHDAGIKDLTLQLEHNRELS 881

BLAST of MELO3C009917 vs. NCBI nr
Match: gi|778685683|ref|XP_011652255.1| (PREDICTED: zinc transporter 5 isoform X1 [Cucumis sativus])

HSP 1 Score: 1397.9 bits (3617), Expect = 0.0e+00
Identity = 699/746 (93.70%), Postives = 712/746 (95.44%), Query Frame = 1

Query: 1   MSQQQLKTMVVKSVLLAIVFLLRFQALLYCGTAAMILAEMTGNVAARFLVEGRNQMTMGD 60
           +SQ QLKTMVVKS+LLAIVFLLRFQALLYCGTAAMILAEMTGNVAARFL E RN+MTMGD
Sbjct: 140 ISQPQLKTMVVKSILLAIVFLLRFQALLYCGTAAMILAEMTGNVAARFLAERRNRMTMGD 199

Query: 61  RTRSRSSEVRGFLSLFFGLFLLSISWDRIDCFPFATSFIDKYGFSVLPRENCMRIWPMLL 120
           RTRSRSSEVRGFLSLF GLFLLSISWDRIDCFPFATSFIDKYGFSVLPRENCMRIWPMLL
Sbjct: 200 RTRSRSSEVRGFLSLFLGLFLLSISWDRIDCFPFATSFIDKYGFSVLPRENCMRIWPMLL 259

Query: 121 PFLSGFLGCYERISMNWGSVKQLGQKRVRLVSLFFTTIILFVPAVISMLLFEAEGKSVSL 180
           PFLSGFLGCYERISMNWGSVKQLGQKRVRLVSLFFTTIILFVPAVISMLLFEAEGKSVS 
Sbjct: 260 PFLSGFLGCYERISMNWGSVKQLGQKRVRLVSLFFTTIILFVPAVISMLLFEAEGKSVSF 319

Query: 181 GSLAWPLANTVVFGVLLNENYSDDKLVSSKDFRSEFLVTFVCTVILELLYFPELSLWGLL 240
           G+LAWPLANTVVFGVLLNENYSDDKLVSSKDFRSEFLVTFVCTVILELLYF ELSLWGLL
Sbjct: 320 GNLAWPLANTVVFGVLLNENYSDDKLVSSKDFRSEFLVTFVCTVILELLYFSELSLWGLL 379

Query: 241 FCGLLLYVAVRELDPVFLNYHELGVESSDSIVTMVMRPVRHILTERKSRKIALFLLINTG 300
           FCGLLLYVAVRELDPV+LNY ELGVESSDSIVTMVMRPVRHILTERKSRKIALFLL+NTG
Sbjct: 380 FCGLLLYVAVRELDPVYLNYLELGVESSDSIVTMVMRPVRHILTERKSRKIALFLLLNTG 439

Query: 301 YMVVEFVAGFMSNSLGLISDACHMLFDCAALAIGLYASYISRLPANNQFNYGRGRFEILS 360
           YMVVEFVAGFMSNSLGLISDACHMLFDCAALAIGLYASYISRLPANNQFNYGRGRFEILS
Sbjct: 440 YMVVEFVAGFMSNSLGLISDACHMLFDCAALAIGLYASYISRLPANNQFNYGRGRFEILS 499

Query: 361 GYANAVFLVLVGALIVLESLERILDPQEISTSSLLTVSIGGLVVNVVGLIFFHEEHHHAH 420
           GYANAVFLVLVGALIVLESLERILDPQEISTSSLLTVSIGGLVVNVVGLIFFHEEHHHAH
Sbjct: 500 GYANAVFLVLVGALIVLESLERILDPQEISTSSLLTVSIGGLVVNVVGLIFFHEEHHHAH 559

Query: 421 GGSGSC----SHSHSHSHSQRNSHSCAGSHHHDKHEHDSCRKHENAISVTKECHESSVSV 480
           GGSGSC    SHSHSHSHS   SHSCAGSHHHDKHEHDS RKHENAISVTKECHESSVS 
Sbjct: 560 GGSGSCAHSHSHSHSHSHSPSKSHSCAGSHHHDKHEHDSGRKHENAISVTKECHESSVS- 619

Query: 481 HTGHHEHRQVDVCSENHLSNHCDDHHHLEHTDHHDHKHDHSHVHHHKHSHDHDHDHHHDC 540
             GHHEH+QVDVCSENHLSN CDDHHH EHTDHHD          HKHSH+HDH H HDC
Sbjct: 620 --GHHEHKQVDVCSENHLSNQCDDHHH-EHTDHHD----------HKHSHNHDHHHQHDC 679

Query: 541 KHEHHDHVHRHDQHGHLSQPKSDVPKMVSHSVSESSHSHPSKQPVGGTVRKKHHHHHIDH 600
           KHEHHDHVH+HDQHGHLS+PKSDVPKMVS+SVSE+SHSHPSKQPV GTVRKKHHHHHIDH
Sbjct: 680 KHEHHDHVHQHDQHGHLSEPKSDVPKMVSYSVSENSHSHPSKQPVEGTVRKKHHHHHIDH 739

Query: 601 NMEGIFLHVLADTMGSVGVVISTLLIKYKGWLVADPACSIFISIMIISSVIPLLRNSAEI 660
           NMEGIFLHVLADTMGSVGVVISTLLIKYKGWLVADPACSIFISIMIISSVIPLLRNSAEI
Sbjct: 740 NMEGIFLHVLADTMGSVGVVISTLLIKYKGWLVADPACSIFISIMIISSVIPLLRNSAEI 799

Query: 661 LLQRVPRAHEQDLKEAVSDIMEIHGVQGIQNLHVWSFTNTDVVGTLRLHVSTETDKPSIK 720
           LLQRVPRAHEQDLKEAV DIMEIHGVQGIQNLHVWSFTNTDVVGTLRLHVSTETDKPSIK
Sbjct: 800 LLQRVPRAHEQDLKEAVRDIMEIHGVQGIQNLHVWSFTNTDVVGTLRLHVSTETDKPSIK 859

Query: 721 AKVEHILHDAGIKDLTLQLEHNRELS 743
           AKVEHILHDAGIKDLTLQLEHN+ELS
Sbjct: 860 AKVEHILHDAGIKDLTLQLEHNQELS 871

BLAST of MELO3C009917 vs. NCBI nr
Match: gi|778685689|ref|XP_011652257.1| (PREDICTED: zinc transporter 5 isoform X3 [Cucumis sativus])

HSP 1 Score: 1394.8 bits (3609), Expect = 0.0e+00
Identity = 697/742 (93.94%), Postives = 712/742 (95.96%), Query Frame = 1

Query: 1   MSQQQLKTMVVKSVLLAIVFLLRFQALLYCGTAAMILAEMTGNVAARFLVEGRNQMTMGD 60
           +SQ QLKTMVVKS+LLAIVFLLRFQALLYCGTAAMILAEMTGNVAARFL E RN+MTMGD
Sbjct: 140 ISQPQLKTMVVKSILLAIVFLLRFQALLYCGTAAMILAEMTGNVAARFLAERRNRMTMGD 199

Query: 61  RTRSRSSEVRGFLSLFFGLFLLSISWDRIDCFPFATSFIDKYGFSVLPRENCMRIWPMLL 120
           RTRSRSSEVRGFLSLF GLFLLSISWDRIDCFPFATSFIDKYGFSVLPRENCMRIWPMLL
Sbjct: 200 RTRSRSSEVRGFLSLFLGLFLLSISWDRIDCFPFATSFIDKYGFSVLPRENCMRIWPMLL 259

Query: 121 PFLSGFLGCYERISMNWGSVKQLGQKRVRLVSLFFTTIILFVPAVISMLLFEAEGKSVSL 180
           PFLSGFLGCYERISMNWGSVKQLGQKRVRLVSLFFTTIILFVPAVISMLLFEAEGKSVS 
Sbjct: 260 PFLSGFLGCYERISMNWGSVKQLGQKRVRLVSLFFTTIILFVPAVISMLLFEAEGKSVSF 319

Query: 181 GSLAWPLANTVVFGVLLNENYSDDKLVSSKDFRSEFLVTFVCTVILELLYFPELSLWGLL 240
           G+LAWPLANTVVFGVLLNENYSDDKLVSSKDFRSEFLVTFVCTVILELLYF ELSLWGLL
Sbjct: 320 GNLAWPLANTVVFGVLLNENYSDDKLVSSKDFRSEFLVTFVCTVILELLYFSELSLWGLL 379

Query: 241 FCGLLLYVAVRELDPVFLNYHELGVESSDSIVTMVMRPVRHILTERKSRKIALFLLINTG 300
           FCGLLLYVAVRELDPV+LNY ELGVESSDSIVTMVMRPVRHILTERKSRKIALFLL+NTG
Sbjct: 380 FCGLLLYVAVRELDPVYLNYLELGVESSDSIVTMVMRPVRHILTERKSRKIALFLLLNTG 439

Query: 301 YMVVEFVAGFMSNSLGLISDACHMLFDCAALAIGLYASYISRLPANNQFNYGRGRFEILS 360
           YMVVEFVAGFMSNSLGLISDACHMLFDCAALAIGLYASYISRLPANNQFNYGRGRFEILS
Sbjct: 440 YMVVEFVAGFMSNSLGLISDACHMLFDCAALAIGLYASYISRLPANNQFNYGRGRFEILS 499

Query: 361 GYANAVFLVLVGALIVLESLERILDPQEISTSSLLTVSIGGLVVNVVGLIFFHEEHHHAH 420
           GYANAVFLVLVGALIVLESLERILDPQEISTSSLLTVSIGGLVVNVVGLIFFHEEHHHAH
Sbjct: 500 GYANAVFLVLVGALIVLESLERILDPQEISTSSLLTVSIGGLVVNVVGLIFFHEEHHHAH 559

Query: 421 GGSGSCSHSHSHSHSQRNSHSCAGSHHHDKHEHDSCRKHENAISVTKECHESSVSVHTGH 480
           GGSGSC+HSHSHS S+  SHSCAGSHHHDKHEHDS RKHENAISVTKECHESSVS   GH
Sbjct: 560 GGSGSCAHSHSHSPSK--SHSCAGSHHHDKHEHDSGRKHENAISVTKECHESSVS---GH 619

Query: 481 HEHRQVDVCSENHLSNHCDDHHHLEHTDHHDHKHDHSHVHHHKHSHDHDHDHHHDCKHEH 540
           HEH+QVDVCSENHLSN CDDHHH EHTDHHD          HKHSH+HDH H HDCKHEH
Sbjct: 620 HEHKQVDVCSENHLSNQCDDHHH-EHTDHHD----------HKHSHNHDHHHQHDCKHEH 679

Query: 541 HDHVHRHDQHGHLSQPKSDVPKMVSHSVSESSHSHPSKQPVGGTVRKKHHHHHIDHNMEG 600
           HDHVH+HDQHGHLS+PKSDVPKMVS+SVSE+SHSHPSKQPV GTVRKKHHHHHIDHNMEG
Sbjct: 680 HDHVHQHDQHGHLSEPKSDVPKMVSYSVSENSHSHPSKQPVEGTVRKKHHHHHIDHNMEG 739

Query: 601 IFLHVLADTMGSVGVVISTLLIKYKGWLVADPACSIFISIMIISSVIPLLRNSAEILLQR 660
           IFLHVLADTMGSVGVVISTLLIKYKGWLVADPACSIFISIMIISSVIPLLRNSAEILLQR
Sbjct: 740 IFLHVLADTMGSVGVVISTLLIKYKGWLVADPACSIFISIMIISSVIPLLRNSAEILLQR 799

Query: 661 VPRAHEQDLKEAVSDIMEIHGVQGIQNLHVWSFTNTDVVGTLRLHVSTETDKPSIKAKVE 720
           VPRAHEQDLKEAV DIMEIHGVQGIQNLHVWSFTNTDVVGTLRLHVSTETDKPSIKAKVE
Sbjct: 800 VPRAHEQDLKEAVRDIMEIHGVQGIQNLHVWSFTNTDVVGTLRLHVSTETDKPSIKAKVE 859

Query: 721 HILHDAGIKDLTLQLEHNRELS 743
           HILHDAGIKDLTLQLEHN+ELS
Sbjct: 860 HILHDAGIKDLTLQLEHNQELS 865

BLAST of MELO3C009917 vs. NCBI nr
Match: gi|658012463|ref|XP_008341499.1| (PREDICTED: zinc transporter 5 [Malus domestica])

HSP 1 Score: 1031.6 bits (2666), Expect = 6.9e-298
Identity = 509/764 (66.62%), Postives = 608/764 (79.58%), Query Frame = 1

Query: 1   MSQQQLKTMVVKSVLLAIVFLLRFQALLYCGTAAMILAEMTGNVAARFLVEGRNQMTMGD 60
           ++Q Q + +V KS+LLA+VFLLRFQAL YCGTAAMILAE++GNVAARFL +G +    GD
Sbjct: 157 ITQSQHRILVAKSILLAVVFLLRFQALRYCGTAAMILAELSGNVAARFLADGHDSSVGGD 216

Query: 61  RTRSRSSEVRGFLSLFFGLFLLSISWDRIDCFPFATSFIDKYGFSVLPRENCMRIWPMLL 120
           R  +RSS+VRGFL+LF GLFLLS+SWDRIDCFPF+   ++  G S+ PR NC+RIWPM+L
Sbjct: 217 R--NRSSKVRGFLALFSGLFLLSMSWDRIDCFPFSAKLVESLGLSLFPRVNCVRIWPMML 276

Query: 121 PFLSGFLGCYERISMNWGSVKQLGQKRVRLVSLFFTTIILFVPAVISMLLFEAEGKSVSL 180
           PFL+GFLGCYER+SMNWG+++QLG+KRVR++SLFFTT++LF+PAV+S  +FEA G  VS+
Sbjct: 277 PFLAGFLGCYERVSMNWGTIRQLGRKRVRVISLFFTTVVLFIPAVVSFFMFEAXGDGVSV 336

Query: 181 GSLAWPLANTVVFGVLLNENYSDDKLVSSKDFRSEFLVTFVCTVILELLYFPELSLWGLL 240
           G+L WPLANTVVFGVLL+E++SDDK +SS DFR E++VTF+CT++LEL YFPELSLWGLL
Sbjct: 337 GTLVWPLANTVVFGVLLSESFSDDKSLSSNDFRREYMVTFLCTLVLELFYFPELSLWGLL 396

Query: 241 FCGLLLYVAVRELDPVFLNYHELGVESSDSIVTMVMRPVRHILTERKSRKIALFLLINTG 300
            CGLLLYVAVRELDP +L Y ELG+ESS+S  T +M+P++HIL+ERKSRKIALFL+INTG
Sbjct: 397 LCGLLLYVAVRELDPYYLKYLELGMESSESFTTAIMKPIKHILSERKSRKIALFLMINTG 456

Query: 301 YMVVEFVAGFMSNSLGLISDACHMLFDCAALAIGLYASYISRLPANNQFNYGRGRFEILS 360
           YM VEF AGFMSNSLGLISDACHMLFDCAALAIGLYASYISRLPANNQ+NYGRGRFE+LS
Sbjct: 457 YMFVEFAAGFMSNSLGLISDACHMLFDCAALAIGLYASYISRLPANNQYNYGRGRFEVLS 516

Query: 361 GYANAVFLVLVGALIVLESLERILDPQEISTSSLLTVSIGGLVVNVVGLIFFHEEHHHAH 420
           GY NAVFLVLVG LIVLES ERILDPQEISTSSLL VSIGGL+VNVVGLIFFHEEHHHAH
Sbjct: 517 GYTNAVFLVLVGLLIVLESFERILDPQEISTSSLLVVSIGGLLVNVVGLIFFHEEHHHAH 576

Query: 421 GGSGSCSHSHSHSH----------------------SQRNSHSCA--GSHHHDKHEHDSC 480
           GGSGSCSHSH H H                      S+ +  SC+    HHH++ +H S 
Sbjct: 577 GGSGSCSHSHHHPHPHDSVGHNHEGHGKHEECIPVFSEDHEKSCSGHHDHHHEQ-QHGSS 636

Query: 481 RKHENAISVTKECHESSVSVHTGHHEHRQVD--VCSENH-LSNHCDDHHHLEHTDHHDHK 540
              ++    +KECH+     H  HH H + D  V S +H   +H   HHH +H   H+H 
Sbjct: 637 VSSKDHNCGSKECHDHQHDQHD-HHGHSKHDSHVHSHHHDQHDHAHQHHHHDHAHQHEH- 696

Query: 541 HDHSHVH-HHKHSHDHDHDHHHDCKHEHHDHVHRHDQHGHLSQPKSDVPKMVSHSVSESS 600
           HDH+H H HH H+H H+H H H  +HEHHDH H+H+ H H +     +P  V+ S  + +
Sbjct: 697 HDHAHQHEHHDHAHLHEH-HDHAHQHEHHDHAHQHEHHDH-AHTHGSLPHQVAESHIQEA 756

Query: 601 HSHPSKQPVGGTVRKKHHHHHIDHNMEGIFLHVLADTMGSVGVVISTLLIKYKGWLVADP 660
             H  KQP+ G    KH H HIDHNMEGIFLHVLADT+GSVGVVISTLLIKYKGWLVADP
Sbjct: 757 GFHSHKQPIDGEKSAKHQHRHIDHNMEGIFLHVLADTLGSVGVVISTLLIKYKGWLVADP 816

Query: 661 ACSIFISIMIISSVIPLLRNSAEILLQRVPRAHEQDLKEAVSDIMEIHGVQGIQNLHVWS 720
           ACSIFIS++IISSVIPLLRNSAEILLQR+PRAHEQDL++A+ ++ +I GV GI+N H+WS
Sbjct: 817 ACSIFISVLIISSVIPLLRNSAEILLQRIPRAHEQDLRKALIEVRKIRGVSGIRNWHIWS 876

Query: 721 FTNTDVVGTLRLHVSTETDKPSIKAKVEHILHDAGIKDLTLQLE 737
           FTN+DVVGTL LHVSTE DK + KAKV H LH+AG+KDLTLQLE
Sbjct: 877 FTNSDVVGTLHLHVSTEIDKAAAKAKVSHXLHEAGVKDLTLQLE 913

BLAST of MELO3C009917 vs. NCBI nr
Match: gi|802574177|ref|XP_012068699.1| (PREDICTED: uncharacterized protein LOC105631249 [Jatropha curcas])

HSP 1 Score: 1031.6 bits (2666), Expect = 6.9e-298
Identity = 512/761 (67.28%), Postives = 602/761 (79.11%), Query Frame = 1

Query: 1   MSQQQLKTMVVKSVLLAIVFLLRFQALLYCGTAAMILAEMTGNVAARFLVE-GRNQMTMG 60
           +S+ Q K +V KS+LLA+VFLLRFQAL YCGTAAMILAE++GNVAARF+ +  R+ +   
Sbjct: 145 ISRSQHKLLVCKSLLLAVVFLLRFQALRYCGTAAMILAELSGNVAARFVADLKRHNLGAT 204

Query: 61  DRTRSRSSEVRGFLSLFFGLFLLSISWDRIDCFPFATSFIDKYGFSVLPRENCMRIWPML 120
                  +++ GFL LF GL LLSISWDRIDCFPF+TSF+ +YGFSV PRENC+RI PML
Sbjct: 205 QNGNYNGNKLFGFLMLFIGLLLLSISWDRIDCFPFSTSFVVRYGFSVFPRENCVRIGPML 264

Query: 121 LPFLSGFLGCYERISMNWGSVKQLGQKRVRLVSLFFTTIILFVPAVISMLLFEAEGKSVS 180
           LPFLSGFLGCY+R+SMNWG++KQLG+KR+RL+SLF+TT++LF+PA +S +LFE +   VS
Sbjct: 265 LPFLSGFLGCYDRLSMNWGTIKQLGEKRIRLISLFYTTVLLFIPAAVSFILFERDNAGVS 324

Query: 181 LGSLAWPLANTVVFGVLLNENYSDDKLVSSKDFRSEFLVTFVCTVILELLYFPELSLWGL 240
            G+  WPLANTVVFGVLL+EN S+DKLV SKDFR EFL+TFVCTV+LEL YFPELSLWGL
Sbjct: 325 FGNFGWPLANTVVFGVLLSENNSEDKLVYSKDFRREFLITFVCTVVLELFYFPELSLWGL 384

Query: 241 LFCGLLLYVAVRELDPVFLNYHELGVESSDSIVTMVMRPVRHILTERKSRKIALFLLINT 300
           L CGLLLY+AV +LDP + NY ELG+E+S  + + +M+P+RHIL+ERKSRKIA FLLINT
Sbjct: 385 LLCGLLLYLAVSKLDPFYSNYLELGLEASQPLSSSIMKPIRHILSERKSRKIAFFLLINT 444

Query: 301 GYMVVEFVAGFMSNSLGLISDACHMLFDCAALAIGLYASYISRLPANNQFNYGRGRFEIL 360
           GYMVVEFVAGFMSNSLGLISDACHMLFDCAALAIGLYASYISRLPANNQFNYGRGRFE+L
Sbjct: 445 GYMVVEFVAGFMSNSLGLISDACHMLFDCAALAIGLYASYISRLPANNQFNYGRGRFEVL 504

Query: 361 SGYANAVFLVLVGALIVLESLERILDPQEISTSSLLTVSIGGLVVNVVGLIFFHEEHHHA 420
           SGY NAVFLVLVGALIVLES ERILDPQEIST+SLLTVSIGGLVVN+VGLIFFHEEHHHA
Sbjct: 505 SGYVNAVFLVLVGALIVLESFERILDPQEISTNSLLTVSIGGLVVNMVGLIFFHEEHHHA 564

Query: 421 HGGSGSCSHSHSHSHSQRNSHSCAGSHHHDKHEHDS-C-RKHENAISVTKECHESSVSVH 480
           HGGSG+CSHSHSH HS  + HS   SHHH  H HD+ C  K    ISV+ +CHE +++ H
Sbjct: 565 HGGSGACSHSHSHPHSHSHPHS--HSHHHHLHSHDNKCHEKDHECISVSGDCHEKTLASH 624

Query: 481 TGHHEHR------QVDVC---------SENHLSNHCDDHHHLEHTDHHDHKHDHSHVHHH 540
           T HHEH+       +D C           NH+ +H   +H   + DHH+H H H   H H
Sbjct: 625 TDHHEHQHCNHHSSIDSCKIESSNCHDDHNHIHDHNPGNHEYHYLDHHEHSHHHHDHHDH 684

Query: 541 KHSH--DHDHDHHHDCK-HEHHDHVHRHDQHGHLSQPKSDVPKMVSHSVSESSH----SH 600
            H H   HDH HHH+ + H HH HVH +  H +     SD+   +S   S S      SH
Sbjct: 685 SHHHHDHHDHSHHHERQDHHHHHHVHGNAPHANTHTYHSDIKSNLSLGNSGSQQVKLVSH 744

Query: 601 PSKQPVGGTVRKKHHHHHIDHNMEGIFLHVLADTMGSVGVVISTLLIKYKGWLVADPACS 660
             +        ++HHHHHIDHNMEGIFLHVLADTMGSVGVV+STLLIKYKGWLVADPACS
Sbjct: 745 EEES-------QEHHHHHIDHNMEGIFLHVLADTMGSVGVVVSTLLIKYKGWLVADPACS 804

Query: 661 IFISIMIISSVIPLLRNSAEILLQRVPRAHEQDLKEAVSDIMEIHGVQGIQNLHVWSFTN 720
           IFIS +I+SSVI LLRNSAEILLQRVPR HEQDL+ A++D+M+I GV+GIQNLHVWSFTN
Sbjct: 805 IFISFLIVSSVIRLLRNSAEILLQRVPRTHEQDLRVAINDVMQIKGVRGIQNLHVWSFTN 864

Query: 721 TDVVGTLRLHVSTETDKPSIKAKVEHILHDAGIKDLTLQLE 737
           TD+VGTL LHVS E DK S+KA+V H+ H AGIKDLT+Q+E
Sbjct: 865 TDIVGTLHLHVSAEADKVSVKAQVLHLFHGAGIKDLTVQVE 896