MELO3C009749 (gene) Melon (DHL92) v3.5.1

NameMELO3C009749
Typegene
OrganismCucumis melo (Melon (DHL92) v3.5.1)
DescriptionPolygalacturonase
Locationchr4 : 28468886 .. 28469184 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAAAAATGTAAATTGGCCAGTACTACAAACGGTGTTCGAATCAAAACATGGCCAGATTCTGTTGGCACGTTCCCAACCACTGACATGCATTTTGAAGATATAGAAATGGTTAATGTGAGTAATCCTATCATCATAGACCAAGAATATTGTCCATGGAACCAATGCAACCGAAAGGTACATCTAAAATTAGGCCTCCTTAGTTTTGAACTTATTAATTAACTCAAAAGACATCAATAATATTATTGTTCGAGTTATGTATTTTTATTATGTTTTCTTTGTTTGTTCAGGTTCCATAA

mRNA sequence

ATGAAAAAATGTAAATTGGCCAGTACTACAAACGGTGTTCGAATCAAAACATGGCCAGATTCTGTTGGCACGTTCCCAACCACTGACATGCATTTTGAAGATATAGAAATGGTTAATGTGAGTAATCCTATCATCATAGACCAAGAATATTGTCCATGGAACCAATGCAACCGAAAGGTTCCATAA

Coding sequence (CDS)

ATGAAAAAATGTAAATTGGCCAGTACTACAAACGGTGTTCGAATCAAAACATGGCCAGATTCTGTTGGCACGTTCCCAACCACTGACATGCATTTTGAAGATATAGAAATGGTTAATGTGAGTAATCCTATCATCATAGACCAAGAATATTGTCCATGGAACCAATGCAACCGAAAGGTTCCATAA

Protein sequence

MKKCKLASTTNGVRIKTWPDSVGTFPTTDMHFEDIEMVNVSNPIIIDQEYCPWNQCNRKVP*
BLAST of MELO3C009749 vs. Swiss-Prot
Match: PGLR_MEDSA (Polygalacturonase OS=Medicago sativa PE=2 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 1.7e-19
Identity = 40/61 (65.57%), Postives = 46/61 (75.41%), Query Frame = 1

Query: 1   MKKCKLASTTNGVRIKTWPDSVGTFPTTDMHFEDIEMVNVSNPIIIDQEYCPWNQCNRKV 60
           +K C L +T NGVRIKTWPD+ GT   +D+HFEDI M NV NP+IIDQEY PWNQC++K 
Sbjct: 263 VKNCTLTATDNGVRIKTWPDAPGTITVSDIHFEDITMTNVKNPVIIDQEYYPWNQCSKKN 322

Query: 61  P 62
           P
Sbjct: 323 P 323

BLAST of MELO3C009749 vs. Swiss-Prot
Match: PGLR_BRANA (Polygalacturonase OS=Brassica napus PE=2 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 1.3e-16
Identity = 36/61 (59.02%), Postives = 42/61 (68.85%), Query Frame = 1

Query: 1   MKKCKLASTTNGVRIKTWPDSVGTFPTTDMHFEDIEMVNVSNPIIIDQEYCPWNQCNRKV 60
           +K C L  T+NG+RIKTWP +  T     +HFEDI +  VSNPI+IDQEYCPWNQCN+  
Sbjct: 254 VKNCTLEGTSNGLRIKTWPSAACTTTAAGIHFEDIILNKVSNPILIDQEYCPWNQCNKNK 313

Query: 61  P 62
           P
Sbjct: 314 P 314

BLAST of MELO3C009749 vs. Swiss-Prot
Match: PGLR_GOSBA (Polygalacturonase OS=Gossypium barbadense GN=G9 PE=2 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 1.8e-13
Identity = 31/58 (53.45%), Postives = 41/58 (70.69%), Query Frame = 1

Query: 1   MKKCKLASTTNGVRIKTWPDSVGTFPTTDMHFEDIEMVNVSNPIIIDQEYCPWNQCNR 59
           +  C + +T+NG RIKTWP   G    +++HFEDI M NVS+PI+IDQ+YCPWN+C +
Sbjct: 265 ISNCTITNTSNGARIKTWPGEHGG-AVSEIHFEDITMNNVSSPILIDQQYCPWNKCKK 321

BLAST of MELO3C009749 vs. Swiss-Prot
Match: PGLR_GOSHI (Polygalacturonase OS=Gossypium hirsutum GN=G9 PE=2 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 1.8e-13
Identity = 31/58 (53.45%), Postives = 41/58 (70.69%), Query Frame = 1

Query: 1   MKKCKLASTTNGVRIKTWPDSVGTFPTTDMHFEDIEMVNVSNPIIIDQEYCPWNQCNR 59
           +  C + +T+NG RIKTWP   G    +++HFEDI M NVS+PI+IDQ+YCPWN+C +
Sbjct: 265 ISNCTITNTSNGARIKTWPGEHGG-AVSEIHFEDITMNNVSSPILIDQQYCPWNKCKK 321

BLAST of MELO3C009749 vs. Swiss-Prot
Match: PGLR_TOBAC (Polygalacturonase OS=Nicotiana tabacum GN=PG1 PE=2 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 2.3e-13
Identity = 33/61 (54.10%), Postives = 42/61 (68.85%), Query Frame = 1

Query: 1   MKKCKLASTTNGVRIKTWPDSVGTFPTTDMHFEDIEMVNVSNPIIIDQEYCPWNQCNRKV 60
           ++ C   +T NGVRIKTWP S       D+HFEDI + NVSNP++IDQ YCP+N+CN+ +
Sbjct: 257 VRNCTFTNTDNGVRIKTWPASHPGV-VNDVHFEDIIVQNVSNPVVIDQVYCPFNKCNKDL 316

Query: 61  P 62
           P
Sbjct: 317 P 316

BLAST of MELO3C009749 vs. TrEMBL
Match: A0A0A0LX75_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G066500 PE=3 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 4.8e-26
Identity = 55/59 (93.22%), Postives = 56/59 (94.92%), Query Frame = 1

Query: 1   MKKCKLASTTNGVRIKTWPDSVGTFPTTDMHFEDIEMVNVSNPIIIDQEYCPWNQCNRK 60
           +KKCKL STTNGVRIKTWPDSVGTFP TDMHFEDIEM NVSNPIIIDQEYCPWNQCNRK
Sbjct: 164 VKKCKLTSTTNGVRIKTWPDSVGTFPATDMHFEDIEMDNVSNPIIIDQEYCPWNQCNRK 222

BLAST of MELO3C009749 vs. TrEMBL
Match: A0A0A0KF15_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G434410 PE=3 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 1.7e-23
Identity = 51/59 (86.44%), Postives = 52/59 (88.14%), Query Frame = 1

Query: 1   MKKCKLASTTNGVRIKTWPDSVGTFPTTDMHFEDIEMVNVSNPIIIDQEYCPWNQCNRK 60
           +KKCKL  TTNGVRIKTWPDS   FP TDMHFEDIEM NVSNPIIIDQEYCPWNQCNRK
Sbjct: 175 VKKCKLTGTTNGVRIKTWPDSAVAFPATDMHFEDIEMDNVSNPIIIDQEYCPWNQCNRK 233

BLAST of MELO3C009749 vs. TrEMBL
Match: E3VSV7_CUCPE (Polygalacturonase OS=Cucurbita pepo GN=PG1 PE=2 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 2.9e-23
Identity = 49/61 (80.33%), Postives = 55/61 (90.16%), Query Frame = 1

Query: 1   MKKCKLASTTNGVRIKTWPDSVGTFPTTDMHFEDIEMVNVSNPIIIDQEYCPWNQCNRKV 60
           +KKCKL  TTNGVRIK+WPDS  ++P +DMHFEDIEM NV+NPIIIDQEYCPWNQCNRKV
Sbjct: 271 VKKCKLIGTTNGVRIKSWPDSAVSYPASDMHFEDIEMENVANPIIIDQEYCPWNQCNRKV 330

Query: 61  P 62
           P
Sbjct: 331 P 331

BLAST of MELO3C009749 vs. TrEMBL
Match: A0A0A0K6J6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G037450 PE=3 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 2.5e-22
Identity = 48/60 (80.00%), Postives = 53/60 (88.33%), Query Frame = 1

Query: 1   MKKCKLASTTNGVRIKTWPDSVGTFPTTDMHFEDIEMVNVSNPIIIDQEYCPWNQCNRKV 60
           +K CKL +TTNGVRIKTWPDS G F  +DMHFEDIEM NVSNP+IIDQEYCPWNQCNRK+
Sbjct: 188 VKACKLINTTNGVRIKTWPDSAGAFIASDMHFEDIEMQNVSNPVIIDQEYCPWNQCNRKL 247

BLAST of MELO3C009749 vs. TrEMBL
Match: A0A0A0KPH7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G266870 PE=3 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 1.0e-20
Identity = 45/57 (78.95%), Postives = 50/57 (87.72%), Query Frame = 1

Query: 3   KCKLASTTNGVRIKTWPDSVGTFPTTDMHFEDIEMVNVSNPIIIDQEYCPWNQCNRK 60
           KCKL  TTNGVRIKTWPDS   +  +DMHFEDIEM++VSNPI+IDQEYCPWNQCNRK
Sbjct: 159 KCKLIKTTNGVRIKTWPDSAVKYSASDMHFEDIEMIDVSNPILIDQEYCPWNQCNRK 215

BLAST of MELO3C009749 vs. TAIR10
Match: AT5G48140.1 (AT5G48140.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 87.4 bits (215), Expect = 3.3e-18
Identity = 36/58 (62.07%), Postives = 43/58 (74.14%), Query Frame = 1

Query: 4   CKLASTTNGVRIKTWPDSVGTFPTTDMHFEDIEMVNVSNPIIIDQEYCPWNQCNRKVP 62
           C L  T NG+RIKTWP +  T   +D+HFE+I + NVSNPI+IDQEYCPWNQCN++ P
Sbjct: 257 CTLQETDNGLRIKTWPSAACTTTASDIHFENILLKNVSNPILIDQEYCPWNQCNKQKP 314

BLAST of MELO3C009749 vs. TAIR10
Match: AT3G07820.1 (AT3G07820.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 84.0 bits (206), Expect = 3.7e-17
Identity = 34/56 (60.71%), Postives = 42/56 (75.00%), Query Frame = 1

Query: 4   CKLASTTNGVRIKTWPDSVGTFPTTDMHFEDIEMVNVSNPIIIDQEYCPWNQCNRK 60
           C L  T NG+RIKTWP +  +   +D+HFEDI + +VSNPI+IDQEYCPWNQCN++
Sbjct: 257 CTLQETDNGLRIKTWPSAACSTTASDIHFEDIILKDVSNPILIDQEYCPWNQCNKQ 312

BLAST of MELO3C009749 vs. TAIR10
Match: AT3G07840.1 (AT3G07840.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 82.4 bits (202), Expect = 1.1e-16
Identity = 34/58 (58.62%), Postives = 41/58 (70.69%), Query Frame = 1

Query: 4   CKLASTTNGVRIKTWPDSVGTFPTTDMHFEDIEMVNVSNPIIIDQEYCPWNQCNRKVP 62
           C L  T NG+RIKTWP +      + +HFE+I + NVSNPI+IDQEYCPWNQCN++ P
Sbjct: 258 CTLQGTDNGLRIKTWPSAACATTASGIHFENIILNNVSNPILIDQEYCPWNQCNKQKP 315

BLAST of MELO3C009749 vs. TAIR10
Match: AT3G07830.1 (AT3G07830.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 81.6 bits (200), Expect = 1.8e-16
Identity = 33/56 (58.93%), Postives = 42/56 (75.00%), Query Frame = 1

Query: 4   CKLASTTNGVRIKTWPDSVGTFPTTDMHFEDIEMVNVSNPIIIDQEYCPWNQCNRK 60
           C L  T NG+RIKTWP +  +   +++HFE+I + NVSNPI+IDQEYCPWNQCN++
Sbjct: 258 CTLQETDNGLRIKTWPSAACSTTASNIHFENIILRNVSNPILIDQEYCPWNQCNKQ 313

BLAST of MELO3C009749 vs. TAIR10
Match: AT4G18180.1 (AT4G18180.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 79.0 bits (193), Expect = 1.2e-15
Identity = 35/61 (57.38%), Postives = 43/61 (70.49%), Query Frame = 1

Query: 1   MKKCKLASTTNGVRIKTWPDSVGTFPTTDMHFEDIEMVNVSNPIIIDQEYCPWNQCNRKV 60
           +K CK++ TTNG+RIKTW +S G    T+M FE+I M NV+NPIIIDQ YCP++ C   V
Sbjct: 270 VKDCKISGTTNGIRIKTWANSPGLSAATNMTFENIIMNNVTNPIIIDQSYCPFSSCISNV 329

Query: 61  P 62
           P
Sbjct: 330 P 330

BLAST of MELO3C009749 vs. NCBI nr
Match: gi|659067859|ref|XP_008441641.1| (PREDICTED: exopolygalacturonase-like [Cucumis melo])

HSP 1 Score: 129.4 bits (324), Expect = 2.2e-27
Identity = 56/61 (91.80%), Postives = 59/61 (96.72%), Query Frame = 1

Query: 1   MKKCKLASTTNGVRIKTWPDSVGTFPTTDMHFEDIEMVNVSNPIIIDQEYCPWNQCNRKV 60
           +KKCKL STTNGVRIKTWPDSVGTFP +DMHFEDIEMVNVSNP+IIDQEYCPWNQCNRKV
Sbjct: 260 VKKCKLTSTTNGVRIKTWPDSVGTFPASDMHFEDIEMVNVSNPVIIDQEYCPWNQCNRKV 319

Query: 61  P 62
           P
Sbjct: 320 P 320

BLAST of MELO3C009749 vs. NCBI nr
Match: gi|778658546|ref|XP_011652893.1| (PREDICTED: exopolygalacturonase-like [Cucumis sativus])

HSP 1 Score: 128.6 bits (322), Expect = 3.7e-27
Identity = 57/61 (93.44%), Postives = 58/61 (95.08%), Query Frame = 1

Query: 1   MKKCKLASTTNGVRIKTWPDSVGTFPTTDMHFEDIEMVNVSNPIIIDQEYCPWNQCNRKV 60
           +KKCKL STTNGVRIKTWPDSVGTFP TDMHFEDIEM NVSNPIIIDQEYCPWNQCNRKV
Sbjct: 262 VKKCKLTSTTNGVRIKTWPDSVGTFPATDMHFEDIEMDNVSNPIIIDQEYCPWNQCNRKV 321

Query: 61  P 62
           P
Sbjct: 322 P 322

BLAST of MELO3C009749 vs. NCBI nr
Match: gi|700209485|gb|KGN64581.1| (hypothetical protein Csa_1G066500 [Cucumis sativus])

HSP 1 Score: 124.4 bits (311), Expect = 6.9e-26
Identity = 55/59 (93.22%), Postives = 56/59 (94.92%), Query Frame = 1

Query: 1   MKKCKLASTTNGVRIKTWPDSVGTFPTTDMHFEDIEMVNVSNPIIIDQEYCPWNQCNRK 60
           +KKCKL STTNGVRIKTWPDSVGTFP TDMHFEDIEM NVSNPIIIDQEYCPWNQCNRK
Sbjct: 164 VKKCKLTSTTNGVRIKTWPDSVGTFPATDMHFEDIEMDNVSNPIIIDQEYCPWNQCNRK 222

BLAST of MELO3C009749 vs. NCBI nr
Match: gi|659134134|ref|XP_008467043.1| (PREDICTED: exopolygalacturonase-like, partial [Cucumis melo])

HSP 1 Score: 120.9 bits (302), Expect = 7.7e-25
Identity = 52/61 (85.25%), Postives = 55/61 (90.16%), Query Frame = 1

Query: 1   MKKCKLASTTNGVRIKTWPDSVGTFPTTDMHFEDIEMVNVSNPIIIDQEYCPWNQCNRKV 60
           +KKCKL  TTNGVRIKTWPDS   FP +DMHFEDIEMVNVSNP+IIDQEYCPWNQCNRKV
Sbjct: 221 VKKCKLTDTTNGVRIKTWPDSAVAFPASDMHFEDIEMVNVSNPVIIDQEYCPWNQCNRKV 280

Query: 61  P 62
           P
Sbjct: 281 P 281

BLAST of MELO3C009749 vs. NCBI nr
Match: gi|659097567|ref|XP_008449696.1| (PREDICTED: LOW QUALITY PROTEIN: exopolygalacturonase-like [Cucumis melo])

HSP 1 Score: 120.2 bits (300), Expect = 1.3e-24
Identity = 51/61 (83.61%), Postives = 55/61 (90.16%), Query Frame = 1

Query: 1   MKKCKLASTTNGVRIKTWPDSVGTFPTTDMHFEDIEMVNVSNPIIIDQEYCPWNQCNRKV 60
           +KKCKL  TTNGVRIKTWPDS   FP +DMHFEDIEMVNVSNP++IDQEYCPWNQCNRKV
Sbjct: 260 VKKCKLTGTTNGVRIKTWPDSAVAFPASDMHFEDIEMVNVSNPVLIDQEYCPWNQCNRKV 319

Query: 61  P 62
           P
Sbjct: 320 P 320

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PGLR_MEDSA1.7e-1965.57Polygalacturonase OS=Medicago sativa PE=2 SV=1[more]
PGLR_BRANA1.3e-1659.02Polygalacturonase OS=Brassica napus PE=2 SV=1[more]
PGLR_GOSBA1.8e-1353.45Polygalacturonase OS=Gossypium barbadense GN=G9 PE=2 SV=1[more]
PGLR_GOSHI1.8e-1353.45Polygalacturonase OS=Gossypium hirsutum GN=G9 PE=2 SV=1[more]
PGLR_TOBAC2.3e-1354.10Polygalacturonase OS=Nicotiana tabacum GN=PG1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LX75_CUCSA4.8e-2693.22Uncharacterized protein OS=Cucumis sativus GN=Csa_1G066500 PE=3 SV=1[more]
A0A0A0KF15_CUCSA1.7e-2386.44Uncharacterized protein OS=Cucumis sativus GN=Csa_6G434410 PE=3 SV=1[more]
E3VSV7_CUCPE2.9e-2380.33Polygalacturonase OS=Cucurbita pepo GN=PG1 PE=2 SV=1[more]
A0A0A0K6J6_CUCSA2.5e-2280.00Uncharacterized protein OS=Cucumis sativus GN=Csa_7G037450 PE=3 SV=1[more]
A0A0A0KPH7_CUCSA1.0e-2078.95Uncharacterized protein OS=Cucumis sativus GN=Csa_5G266870 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G48140.13.3e-1862.07 Pectin lyase-like superfamily protein[more]
AT3G07820.13.7e-1760.71 Pectin lyase-like superfamily protein[more]
AT3G07840.11.1e-1658.62 Pectin lyase-like superfamily protein[more]
AT3G07830.11.8e-1658.93 Pectin lyase-like superfamily protein[more]
AT4G18180.11.2e-1557.38 Pectin lyase-like superfamily protein[more]
Match NameE-valueIdentityDescription
gi|659067859|ref|XP_008441641.1|2.2e-2791.80PREDICTED: exopolygalacturonase-like [Cucumis melo][more]
gi|778658546|ref|XP_011652893.1|3.7e-2793.44PREDICTED: exopolygalacturonase-like [Cucumis sativus][more]
gi|700209485|gb|KGN64581.1|6.9e-2693.22hypothetical protein Csa_1G066500 [Cucumis sativus][more]
gi|659134134|ref|XP_008467043.1|7.7e-2585.25PREDICTED: exopolygalacturonase-like, partial [Cucumis melo][more]
gi|659097567|ref|XP_008449696.1|1.3e-2483.61PREDICTED: LOW QUALITY PROTEIN: exopolygalacturonase-like [Cucumis melo][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000743Glyco_hydro_28
IPR011050Pectin_lyase_fold/virulence
IPR012334Pectin_lyas_fold
Vocabulary: Molecular Function
TermDefinition
GO:0004650polygalacturonase activity
Vocabulary: Biological Process
TermDefinition
GO:0005975carbohydrate metabolic process
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071555 cell wall organization
biological_process GO:0009094 L-phenylalanine biosynthetic process
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
biological_process GO:0000162 tryptophan biosynthetic process
biological_process GO:0006571 tyrosine biosynthetic process
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0005576 extracellular region
molecular_function GO:0004106 chorismate mutase activity
molecular_function GO:0004650 polygalacturonase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO3C009749T1MELO3C009749T1mRNA


Analysis Name: InterPro Annotations of melon
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 2..59
score: 1.3
IPR011050Pectin lyase fold/virulence factorunknownSSF51126Pectin lyase-likecoord: 3..58
score: 4.54
IPR012334Pectin lyase foldGENE3DG3DSA:2.160.20.10coord: 2..58
score: 3.8
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 1..61
score: 9.6
NoneNo IPR availablePANTHERPTHR31375:SF37PECTIN LYASE-LIKE SUPERFAMILY PROTEIN-RELATEDcoord: 1..61
score: 9.6