MELO3C009695 (gene) Melon (DHL92) v3.5.1

NameMELO3C009695
Typegene
OrganismCucumis melo (Melon (DHL92) v3.5.1)
DescriptionLeucine-rich repeat (LRR) family protein
Locationchr4 : 28904135 .. 28906729 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCGAGCCTCAAAGCAATTCTTTTGGATGCTGAAGAGAAGCAAGAACAAAATCAACGTCTACATGATTGGTTAAAGGAACTTCAAAATGTCTTTTACCAAGTTGAGGACTCCATAGATGAATTCAAATGGGAATTCTTCAAACAAAAGGACACTGGAAAACAGGTACTTGCCCATTTCTCGTGCTCTAGTCGAATTTCTGCAAATAAATTGAAACAGAATTTTAAAAGGAACAAAGTATGCGACGAGCTCAATAAAATTGCAGCCAACATGTATGAATTTCATCTCAAAGTAAAGCACATCGGTTCCATAAGTATGGAGACAACACACTCTTTCCCTAGTGCTTCAGAAATTTCAACAAGACACCTGAAACCAAGTTGGCAATTGCTTTACCCTTTAAAACATGCTTCCAGAAATTATGACGAAGCATATGATGGCATTTTGAATGTTTTCAATAAATATACTCATGGGTTCTTCCACATAGTTGGGGAAGCAGGTATAGGTAAGAGCACAATGGCCAGATTCTTGTACAATCATGAAAATGTAGTTGATAGGTATACTAGAAGATACTGGGTTTGTGTGGAAGAAGGCTTTAATACACATAGATTGATGAAAGAGATTTACAGTCATGCAGACAATAAAGAAACTCGGGAGGACTTGACGACAGAACAATTGCTTTCTAAGTTTATACGACTTCAGAGAGAGGAAACCTTTTTGCTCGTCTTTCAAGACCTTTCAATCACCAACTTGGATAAGTGTTCCACTTCAGTGTTAATTAAATTATTGGAGATGGGACACCATGACAGCAAGATCATAGTGACCACACAAACTGAGGAAATTGCCAAAGATATAGATGTCCATGGCTACAAGACTGAGATACAATCAAAGAAAAATCTGGCGAATGGTGACGAAGTATCTGCAGACAAACAGTCATTAACAAAAAATACTCAGAACCAAGCAGTTCCAGGTTCACAAACTGAGGAAACTGCAGGCGCTATAGCAAACATGATCTATGAGACTGAGAAAAACTTAGAGAAAAATCCGGTGGCTGGTGACGGAGAATCTGAAAGCAGCCGATTGATAACAAAAGATGCTCAGAATCTAGACGTTTCAATTCCACAAGTTACAGAATGTGAACCTGATAGGGGATATCAAACAATTTTCAAACTTAAGAAGCTGTCAAAGCAAAGTTCATCTCTTTTATTCAAAGAATATGCTTTCAGAAACAGTCCAGAAGTACAAAATCCAGAACTCACTAAAATAGTTGATCAACTTTTGGAGAAATGCATGGGAGTTCCTTTGGCAATAAAGTGTCTGGGAAGCTTGCTATCTTCAAAAACTAGCATAGCTGAGTGGAAAAACATCGAGAAAAAGTTGATGCCCCAAGAGAAAAAGGAAGACGGTATTTTACATGTACTCAGAGTTTGCTATGATCAAATGCCCTCACAATTGAAGCCTTGTTTCCTGTATTGTTCTCAATTACCAAACGATCGCATATTTTCTTCAAATGATATGATTCAGTTATGGATGGCAAATGGGCTCCTCCATTCACCTGAAGAGAAGAACTTAGCTATGGAAAATATAGGTAAGAAGTACTTCATGGAGCTATGGTCAAGATGTTTCATTCAAGAAATTGAAGAACATGGGCTTGGCTACTGGATTAAATTGCACCCTCTCATCCAAAAACTTGCACACAAAATCACACAAGAACAATCTGAGGGCTCGTGGGGCAACAATCATTCCAAAGAAATCACTGAAATAAGATCCATAGCCTTTCAAGAAAGAAATATGGTGCTACCTAATGCATCCCTAACTGAAAAGTGCATCTGGAGGTACAAATGCTTAAGATTGTTGTATTTAGGCAATGCAGACCTTCAGGAAATTCCAAATTCCATAGGAACACTCAAGTACCTGAGATACCTCGACTTGCATGGCAATCAGAAAATCAAGCATCTACCAGACTCAATATGTGATCTACAAAGTTTGCAAACCTTAATTCTTGATTCTTGTTCCGCACTTGAAGACCTGCCGAAGGATATAAGGAATTTGATCAGCCTGAGATACTTGTGGGTAACAACAAACAAGCTTCATCTGCACAAAAACGGAGTTGGAACCATGACCTCTCTGCGATTTCTTGCCATTGGAGGGTGCAAGAACCTAGAAAATCTATTTGAACGGCCAGATTGCCTTGCAGGCCTTGAAACCCTAATGATATATAATTGCAATACCTTGAAATTGTTGCCAAACGAGATGAGGTATCTAAAATCACTACAAAATTTGATGATTTGGAGATGCAAGCAACTTACACTAAACTTAGAAGAAGTCGAATTCAAGCTTCAAAGATTCACGGTCAAGGAGCTTCCAAAAGTGGAAAGCTTACCTCGATGGCTTGAAAATTCGATAGAGACTTTGAGAACCTTGCAGATCATCAATTGTCCCATAAGATTAATGGAACTACCGGTAGATAAAAAGTATAGAGCACTGGAAATTTTCTTAATTCATGGTTCGGTAAGGTTTGATACAATGCCAGATTACGACTTTGAACACCGGAATTTGACAATCTTTCGTGGTAATGAGATGAATATACATTATTAG

mRNA sequence

ATGTCGAGCCTCAAAGCAATTCTTTTGGATGCTGAAGAGAAGCAAGAACAAAATCAACGTCTACATGATTGGTTAAAGGAACTTCAAAATGTCTTTTACCAAGTTGAGGACTCCATAGATGAATTCAAATGGGAATTCTTCAAACAAAAGGACACTGGAAAACAGGTACTTGCCCATTTCTCGTGCTCTAGTCGAATTTCTGCAAATAAATTGAAACAGAATTTTAAAAGGAACAAAGTATGCGACGAGCTCAATAAAATTGCAGCCAACATGTATGAATTTCATCTCAAAGTAAAGCACATCGGTTCCATAAGTATGGAGACAACACACTCTTTCCCTAGTGCTTCAGAAATTTCAACAAGACACCTGAAACCAAGTTGGCAATTGCTTTACCCTTTAAAACATGCTTCCAGAAATTATGACGAAGCATATGATGGCATTTTGAATGTTTTCAATAAATATACTCATGGGTTCTTCCACATAGTTGGGGAAGCAGGTATAGGTAAGAGCACAATGGCCAGATTCTTGTACAATCATGAAAATGTAGTTGATAGGTATACTAGAAGATACTGGGTTTGTGTGGAAGAAGGCTTTAATACACATAGATTGATGAAAGAGATTTACAGTCATGCAGACAATAAAGAAACTCGGGAGGACTTGACGACAGAACAATTGCTTTCTAAGTTTATACGACTTCAGAGAGAGGAAACCTTTTTGCTCGTCTTTCAAGACCTTTCAATCACCAACTTGGATAAGTGTTCCACTTCAGTGTTAATTAAATTATTGGAGATGGGACACCATGACAGCAAGATCATAGTGACCACACAAACTGAGGAAATTGCCAAAGATATAGATGTCCATGGCTACAAGACTGAGATACAATCAAAGAAAAATCTGGCGAATGGTGACGAAGTATCTGCAGACAAACAGTCATTAACAAAAAATACTCAGAACCAAGCAGTTCCAGGTTCACAAACTGAGGAAACTGCAGGCGCTATAGCAAACATGATCTATGAGACTGAGAAAAACTTAGAGAAAAATCCGGTGGCTGGTGACGGAGAATCTGAAAGCAGCCGATTGATAACAAAAGATGCTCAGAATCTAGACGTTTCAATTCCACAAGTTACAGAATGTGAACCTGATAGGGGATATCAAACAATTTTCAAACTTAAGAAGCTGTCAAAGCAAAGTTCATCTCTTTTATTCAAAGAATATGCTTTCAGAAACAGTCCAGAAGTACAAAATCCAGAACTCACTAAAATAGTTGATCAACTTTTGGAGAAATGCATGGGAGTTCCTTTGGCAATAAAGTGTCTGGGAAGCTTGCTATCTTCAAAAACTAGCATAGCTGAGTGGAAAAACATCGAGAAAAAGTTGATGCCCCAAGAGAAAAAGGAAGACGGTATTTTACATGTACTCAGAGTTTGCTATGATCAAATGCCCTCACAATTGAAGCCTTGTTTCCTGTATTGTTCTCAATTACCAAACGATCGCATATTTTCTTCAAATGATATGATTCAGTTATGGATGGCAAATGGGCTCCTCCATTCACCTGAAGAGAAGAACTTAGCTATGGAAAATATAGGTAAGAAGTACTTCATGGAGCTATGGTCAAGATGTTTCATTCAAGAAATTGAAGAACATGGGCTTGGCTACTGGATTAAATTGCACCCTCTCATCCAAAAACTTGCACACAAAATCACACAAGAACAATCTGAGGGCTCGTGGGGCAACAATCATTCCAAAGAAATCACTGAAATAAGATCCATAGCCTTTCAAGAAAGAAATATGGTGCTACCTAATGCATCCCTAACTGAAAAGTGCATCTGGAGGTACAAATGCTTAAGATTGTTGTATTTAGGCAATGCAGACCTTCAGGAAATTCCAAATTCCATAGGAACACTCAAGTACCTGAGATACCTCGACTTGCATGGCAATCAGAAAATCAAGCATCTACCAGACTCAATATGTGATCTACAAAGTTTGCAAACCTTAATTCTTGATTCTTGTTCCGCACTTGAAGACCTGCCGAAGGATATAAGGAATTTGATCAGCCTGAGATACTTGTGGGTAACAACAAACAAGCTTCATCTGCACAAAAACGGAGTTGGAACCATGACCTCTCTGCGATTTCTTGCCATTGGAGGGTGCAAGAACCTAGAAAATCTATTTGAACGGCCAGATTGCCTTGCAGGCCTTGAAACCCTAATGATATATAATTGCAATACCTTGAAATTGTTGCCAAACGAGATGAGGTATCTAAAATCACTACAAAATTTGATGATTTGGAGATGCAAGCAACTTACACTAAACTTAGAAGAAGTCGAATTCAAGCTTCAAAGATTCACGGTCAAGGAGCTTCCAAAAGTGGAAAGCTTACCTCGATGGCTTGAAAATTCGATAGAGACTTTGAGAACCTTGCAGATCATCAATTGTCCCATAAGATTAATGGAACTACCGGTAGATAAAAAGTATAGAGCACTGGAAATTTTCTTAATTCATGGTTCGGTAAGGTTTGATACAATGCCAGATTACGACTTTGAACACCGGAATTTGACAATCTTTCGTGGTAATGAGATGAATATACATTATTAG

Coding sequence (CDS)

ATGTCGAGCCTCAAAGCAATTCTTTTGGATGCTGAAGAGAAGCAAGAACAAAATCAACGTCTACATGATTGGTTAAAGGAACTTCAAAATGTCTTTTACCAAGTTGAGGACTCCATAGATGAATTCAAATGGGAATTCTTCAAACAAAAGGACACTGGAAAACAGGTACTTGCCCATTTCTCGTGCTCTAGTCGAATTTCTGCAAATAAATTGAAACAGAATTTTAAAAGGAACAAAGTATGCGACGAGCTCAATAAAATTGCAGCCAACATGTATGAATTTCATCTCAAAGTAAAGCACATCGGTTCCATAAGTATGGAGACAACACACTCTTTCCCTAGTGCTTCAGAAATTTCAACAAGACACCTGAAACCAAGTTGGCAATTGCTTTACCCTTTAAAACATGCTTCCAGAAATTATGACGAAGCATATGATGGCATTTTGAATGTTTTCAATAAATATACTCATGGGTTCTTCCACATAGTTGGGGAAGCAGGTATAGGTAAGAGCACAATGGCCAGATTCTTGTACAATCATGAAAATGTAGTTGATAGGTATACTAGAAGATACTGGGTTTGTGTGGAAGAAGGCTTTAATACACATAGATTGATGAAAGAGATTTACAGTCATGCAGACAATAAAGAAACTCGGGAGGACTTGACGACAGAACAATTGCTTTCTAAGTTTATACGACTTCAGAGAGAGGAAACCTTTTTGCTCGTCTTTCAAGACCTTTCAATCACCAACTTGGATAAGTGTTCCACTTCAGTGTTAATTAAATTATTGGAGATGGGACACCATGACAGCAAGATCATAGTGACCACACAAACTGAGGAAATTGCCAAAGATATAGATGTCCATGGCTACAAGACTGAGATACAATCAAAGAAAAATCTGGCGAATGGTGACGAAGTATCTGCAGACAAACAGTCATTAACAAAAAATACTCAGAACCAAGCAGTTCCAGGTTCACAAACTGAGGAAACTGCAGGCGCTATAGCAAACATGATCTATGAGACTGAGAAAAACTTAGAGAAAAATCCGGTGGCTGGTGACGGAGAATCTGAAAGCAGCCGATTGATAACAAAAGATGCTCAGAATCTAGACGTTTCAATTCCACAAGTTACAGAATGTGAACCTGATAGGGGATATCAAACAATTTTCAAACTTAAGAAGCTGTCAAAGCAAAGTTCATCTCTTTTATTCAAAGAATATGCTTTCAGAAACAGTCCAGAAGTACAAAATCCAGAACTCACTAAAATAGTTGATCAACTTTTGGAGAAATGCATGGGAGTTCCTTTGGCAATAAAGTGTCTGGGAAGCTTGCTATCTTCAAAAACTAGCATAGCTGAGTGGAAAAACATCGAGAAAAAGTTGATGCCCCAAGAGAAAAAGGAAGACGGTATTTTACATGTACTCAGAGTTTGCTATGATCAAATGCCCTCACAATTGAAGCCTTGTTTCCTGTATTGTTCTCAATTACCAAACGATCGCATATTTTCTTCAAATGATATGATTCAGTTATGGATGGCAAATGGGCTCCTCCATTCACCTGAAGAGAAGAACTTAGCTATGGAAAATATAGGTAAGAAGTACTTCATGGAGCTATGGTCAAGATGTTTCATTCAAGAAATTGAAGAACATGGGCTTGGCTACTGGATTAAATTGCACCCTCTCATCCAAAAACTTGCACACAAAATCACACAAGAACAATCTGAGGGCTCGTGGGGCAACAATCATTCCAAAGAAATCACTGAAATAAGATCCATAGCCTTTCAAGAAAGAAATATGGTGCTACCTAATGCATCCCTAACTGAAAAGTGCATCTGGAGGTACAAATGCTTAAGATTGTTGTATTTAGGCAATGCAGACCTTCAGGAAATTCCAAATTCCATAGGAACACTCAAGTACCTGAGATACCTCGACTTGCATGGCAATCAGAAAATCAAGCATCTACCAGACTCAATATGTGATCTACAAAGTTTGCAAACCTTAATTCTTGATTCTTGTTCCGCACTTGAAGACCTGCCGAAGGATATAAGGAATTTGATCAGCCTGAGATACTTGTGGGTAACAACAAACAAGCTTCATCTGCACAAAAACGGAGTTGGAACCATGACCTCTCTGCGATTTCTTGCCATTGGAGGGTGCAAGAACCTAGAAAATCTATTTGAACGGCCAGATTGCCTTGCAGGCCTTGAAACCCTAATGATATATAATTGCAATACCTTGAAATTGTTGCCAAACGAGATGAGGTATCTAAAATCACTACAAAATTTGATGATTTGGAGATGCAAGCAACTTACACTAAACTTAGAAGAAGTCGAATTCAAGCTTCAAAGATTCACGGTCAAGGAGCTTCCAAAAGTGGAAAGCTTACCTCGATGGCTTGAAAATTCGATAGAGACTTTGAGAACCTTGCAGATCATCAATTGTCCCATAAGATTAATGGAACTACCGGTAGATAAAAAGTATAGAGCACTGGAAATTTTCTTAATTCATGGTTCGGTAAGGTTTGATACAATGCCAGATTACGACTTTGAACACCGGAATTTGACAATCTTTCGTGGTAATGAGATGAATATACATTATTAG

Protein sequence

MSSLKAILLDAEEKQEQNQRLHDWLKELQNVFYQVEDSIDEFKWEFFKQKDTGKQVLAHFSCSSRISANKLKQNFKRNKVCDELNKIAANMYEFHLKVKHIGSISMETTHSFPSASEISTRHLKPSWQLLYPLKHASRNYDEAYDGILNVFNKYTHGFFHIVGEAGIGKSTMARFLYNHENVVDRYTRRYWVCVEEGFNTHRLMKEIYSHADNKETREDLTTEQLLSKFIRLQREETFLLVFQDLSITNLDKCSTSVLIKLLEMGHHDSKIIVTTQTEEIAKDIDVHGYKTEIQSKKNLANGDEVSADKQSLTKNTQNQAVPGSQTEETAGAIANMIYETEKNLEKNPVAGDGESESSRLITKDAQNLDVSIPQVTECEPDRGYQTIFKLKKLSKQSSSLLFKEYAFRNSPEVQNPELTKIVDQLLEKCMGVPLAIKCLGSLLSSKTSIAEWKNIEKKLMPQEKKEDGILHVLRVCYDQMPSQLKPCFLYCSQLPNDRIFSSNDMIQLWMANGLLHSPEEKNLAMENIGKKYFMELWSRCFIQEIEEHGLGYWIKLHPLIQKLAHKITQEQSEGSWGNNHSKEITEIRSIAFQERNMVLPNASLTEKCIWRYKCLRLLYLGNADLQEIPNSIGTLKYLRYLDLHGNQKIKHLPDSICDLQSLQTLILDSCSALEDLPKDIRNLISLRYLWVTTNKLHLHKNGVGTMTSLRFLAIGGCKNLENLFERPDCLAGLETLMIYNCNTLKLLPNEMRYLKSLQNLMIWRCKQLTLNLEEVEFKLQRFTVKELPKVESLPRWLENSIETLRTLQIINCPIRLMELPVDKKYRALEIFLIHGSVRFDTMPDYDFEHRNLTIFRGNEMNIHY*
BLAST of MELO3C009695 vs. Swiss-Prot
Match: RGA1_SOLBU (Putative disease resistance protein RGA1 OS=Solanum bulbocastanum GN=RGA1 PE=2 SV=2)

HSP 1 Score: 186.4 bits (472), Expect = 1.3e-45
Identity = 122/337 (36.20%), Postives = 178/337 (52.82%), Query Frame = 1

Query: 388 FKLKKLSKQSSSLLFKEYAFRNSPEVQNPELTKIVDQLLEKCMGVPLAIKCLGSLLSSKT 447
           ++L  LS +    LF + AF +  E+ NP L  I  ++++KC GVPLA K LG +L  K 
Sbjct: 306 YELSNLSPEDCWFLFMQRAFGHQEEI-NPNLMAIGKEIVKKCGGVPLAAKTLGGILRFKR 365

Query: 448 SIAEWKNIEKKL---MPQEKKEDGILHVLRVCYDQMPSQLKPCFLYCSQLPNDRIFSSND 507
              EW+++       +PQ+  E  IL  LR+ Y  +P  L+ CF+YC+  P D   +  +
Sbjct: 366 EEREWEHVRDSPIWNLPQD--ESSILPALRLSYHHLPLDLRQCFVYCAVFPKDTKMAKEN 425

Query: 508 MIQLWMANGLLHSPEEKNLAMENIGKKYFMELWSRCFIQEIEEHGLGYWIKLHPLIQKLA 567
           +I  WMA+G L S  + NL +E++G + + EL+ R F QEIE      + K+H LI  LA
Sbjct: 426 LIAFWMAHGFLLS--KGNLELEDVGNEVWNELYLRSFFQEIEVESGKTYFKMHDLIHDLA 485

Query: 568 HKITQEQSEGSWGNNHSKEITEIR--------SIAFQERNMVLPNASLTEKCIWRYKCLR 627
             +          N  S  I EI         SI F E       +S +   + ++  LR
Sbjct: 486 TSLFS-------ANTSSSNIREINANYDGYMMSIGFAE-----VVSSYSPSLLQKFVSLR 545

Query: 628 LLYLGNADLQEIPNSIGTLKYLRYLDLHGNQKIKHLPDSICDLQSLQTLILDSCSALEDL 687
           +L L N++L ++P+SIG L +LRYLDL GN +I++LP  +C LQ+LQTL L  C +L  L
Sbjct: 546 VLNLRNSNLNQLPSSIGDLVHLRYLDLSGNFRIRNLPKRLCKLQNLQTLDLHYCDSLSCL 605

Query: 688 PKDIRNLISLRYLWVTTNKLHLHKNGVGTMTSLRFLA 714
           PK    L SLR L +    L      +G +T L+ L+
Sbjct: 606 PKQTSKLGSLRNLLLDGCSLTSTPPRIGLLTCLKSLS 625


HSP 2 Score: 95.1 bits (235), Expect = 3.9e-18
Identity = 71/283 (25.09%), Postives = 136/283 (48.06%), Query Frame = 1

Query: 2   SSLKAILLDAEEKQEQNQRLHDWLKELQNVFYQVEDSIDEFKWEF--FKQKDTGKQVLAH 61
           S+++A+L DA+EKQ  ++ L +WL++L    Y+V+D +DE+K +   F Q + G+     
Sbjct: 39  STIQAVLEDAQEKQLNDKPLENWLQKLNAATYEVDDILDEYKTKATRFLQSEYGRY---- 98

Query: 62  FSCSSRISANKLKQNFKRNKVCDELNKIAANMYEFHLKVKHI-GSISMETTHSFPSASEI 121
                ++   + K   + ++V  +LN IA    +FHL+ K I    +   T S  +  ++
Sbjct: 99  ---HPKVIPFRHKVGKRMDQVMKKLNAIAEERKKFHLQEKIIERQAATRETGSVLTEPQV 158

Query: 122 STRHLKPSWQLLYPLKHASRNYDEAYDGILNVFNKYTH-GFFHIVGEAGIGKSTMARFLY 181
             R                +  DE    ++N  +         I+G  G+GK+T+++ ++
Sbjct: 159 YGR---------------DKEKDEIVKILINTASDAQKLSVLPILGMGGLGKTTLSQMVF 218

Query: 182 NHENVVDRYTRRYWVCVEEGFNTHRLMKEIYSHADNKETREDLTTEQLLSKFIRLQREET 241
           N + V +R+  + W+C+ + FN  RL+K I    + K +  D+    L  K   L   + 
Sbjct: 219 NDQRVTERFYPKIWICISDDFNEKRLIKAIVESIEGK-SLSDMDLAPLQKKLQELLNGKR 278

Query: 242 FLLVFQDLSITNLDKCSTSVLIKLLEMGHHDSKIIVTTQTEEI 281
           + LV  D  + N D+   + L  +L++G   + ++ TT+ E++
Sbjct: 279 YFLVLDD--VWNEDQHKWANLRAVLKVGASGAFVLTTTRLEKV 296


HSP 3 Score: 47.0 bits (110), Expect = 1.2e-03
Identity = 35/121 (28.93%), Postives = 53/121 (43.80%), Query Frame = 1

Query: 622 NADLQEIPNSI-GTLKYLRYLDLHGNQKIKHLPDSICDLQSLQTLILDSCSALEDLPKDI 681
           N +   +P  +  +L  L+YL +   + +K LP S+  L +L++L  + C ALE LP++ 
Sbjct: 853 NVEATSLPEEMFKSLANLKYLKISFFRNLKELPTSLASLNALKSLKFEFCDALESLPEE- 912

Query: 682 RNLISLRYLWVTTNKLHLHKNGVGTMTSLRFLAIGGCKNLENLFERPDCLAGLETLMIYN 741
                                GV  +TSL  L++  C  L+ L E    L  L TL I  
Sbjct: 913 ---------------------GVKGLTSLTELSVSNCMMLKCLPEGLQHLTALTTLTITQ 951

BLAST of MELO3C009695 vs. Swiss-Prot
Match: RGA3_SOLBU (Putative disease resistance protein RGA3 OS=Solanum bulbocastanum GN=RGA3 PE=2 SV=2)

HSP 1 Score: 181.0 bits (458), Expect = 5.4e-44
Identity = 117/337 (34.72%), Postives = 184/337 (54.60%), Query Frame = 1

Query: 383 GYQTIFKLKKLSKQSSSLLFKEYAFRNSPEVQNPELTKIVDQLLEKCMGVPLAIKCLGSL 442
           G   +++L  LS++   LLFK+ AF +  E  +P+L +I  ++++KC GVPLA K LG L
Sbjct: 301 GTLQLYQLSNLSQEDCWLLFKQRAFCHQTET-SPKLMEIGKEIVKKCGGVPLAAKTLGGL 360

Query: 443 LSSKTSIAEWKNI-EKKLMPQEKKEDGILHVLRVCYDQMPSQLKPCFLYCSQLPNDRIFS 502
           L  K   +EW+++ + ++    + E+ +L  LR+ Y  +P  L+ CF YC+  P D    
Sbjct: 361 LRFKREESEWEHVRDSEIWNLPQDENSVLPALRLSYHHLPLDLRQCFAYCAVFPKDTKIE 420

Query: 503 SNDMIQLWMANGLLHSPEEKNLAMENIGKKYFMELWSRCFIQEIEEHGLGYWIKLHPLIQ 562
              +I LWMA+  L S  + N+ +E++G + + EL+ R F QEIE      + K+H LI 
Sbjct: 421 KEYLIALWMAHSFLLS--KGNMELEDVGNEVWNELYLRSFFQEIEVKSGKTYFKMHDLIH 480

Query: 563 KLAHKI-TQEQSEGSWGNNHSKEITEIRSIAFQERNMVLPN-----ASLTEKCIWRYKCL 622
            LA  + +   S  S    + K+  ++  I    ++M+        +S +     R+  L
Sbjct: 481 DLATSMFSASASSRSIRQINVKDDEDMMFIVTNYKDMMSIGFSEVVSSYSPSLFKRFVSL 540

Query: 623 RLLYLGNADLQEIPNSIGTLKYLRYLDLHGNQKIKHLPDSICDLQSLQTLILDSCSALED 682
           R+L L N++ +++P+S+G L +LRYLDL GN KI  LP  +C LQ+LQTL L +C +L  
Sbjct: 541 RVLNLSNSEFEQLPSSVGDLVHLRYLDLSGN-KICSLPKRLCKLQNLQTLDLYNCQSLSC 600

Query: 683 LPKDIRNLISLRYLWVTTNKLHLHKNGVGTMTSLRFL 713
           LPK    L SLR L +    L      +G +T L+ L
Sbjct: 601 LPKQTSKLCSLRNLVLDHCPLTSMPPRIGLLTCLKTL 633


HSP 2 Score: 122.5 bits (306), Expect = 2.3e-26
Identity = 104/384 (27.08%), Postives = 169/384 (44.01%), Query Frame = 1

Query: 383 GYQTIFKLKKLSKQSSSLLFKEYAFRNSPEVQNPELTKIVDQLLEKCMGVPLAIKCLGSL 442
           G   +++L  LS++   LLFK+ AF +  E  +P+L +I  ++++KC GVPLA K LG L
Sbjct: 301 GTLQLYQLSNLSQEDCWLLFKQRAFCHQTET-SPKLMEIGKEIVKKCGGVPLAAKTLGGL 360

Query: 443 LSSKTSIAEWKNI-EKKLMPQEKKEDGILHVLRVCYDQMPSQLKPCFLYCSQLPNDRIFS 502
           L  K   +EW+++ + ++    + E+ +L  LR+ Y  +P  L+ CF YC+  P D    
Sbjct: 361 LRFKREESEWEHVRDSEIWNLPQDENSVLPALRLSYHHLPLDLRQCFAYCAVFPKDTKIE 420

Query: 503 SNDMIQLWMANGLLHSPEEKNLAMENIGKKYFMELWSRCFIQEIEEHGLGYWIKLHPLIQ 562
              +I LWMA+  L S  + N+ +E++G + + EL+ R F QEIE               
Sbjct: 421 KEYLIALWMAHSFLLS--KGNMELEDVGNEVWNELYLRSFFQEIEV-------------- 480

Query: 563 KLAHKITQEQSEGSWGNNHSKEITEIRSIAFQERNMVLPNASLTEKCIWRYKCLRLLYLG 622
                          G  + K    I  +A    +    + S+ +  +   + +  +   
Sbjct: 481 -------------KSGKTYFKMHDLIHDLATSMFSASASSRSIRQINVKDDEDMMFIVTN 540

Query: 623 NADLQEIPNSIGTLKYLRYLDLHGNQKIKHLPDSICDLQSLQTLILDSCSALEDLPKDIR 682
             D+  I  S     Y               P       SL+ L L + S  E LP  + 
Sbjct: 541 YKDMMSIGFSEVVSSYS--------------PSLFKRFVSLRVLNLSN-SEFEQLPSSVG 600

Query: 683 NLISLRYLWVTTNKLHLHKNGVGTMTSLRFLAIGGCKNLENLFERPDCLAGLETLMIYNC 742
           +L+ LRYL ++ NK+                    C    +L +R   L  L+TL +YNC
Sbjct: 601 DLVHLRYLDLSGNKI--------------------C----SLPKRLCKLQNLQTLDLYNC 615

Query: 743 NTLKLLPNEMRYLKSLQNLMIWRC 766
            +L  LP +   L SL+NL++  C
Sbjct: 661 QSLSCLPKQTSKLCSLRNLVLDHC 615


HSP 3 Score: 88.2 bits (217), Expect = 4.8e-16
Identity = 71/281 (25.27%), Postives = 130/281 (46.26%), Query Frame = 1

Query: 2   SSLKAILLDAEEKQEQNQRLHDWLKELQNVFYQVEDSIDEFKWEFFKQKDTGKQVLAHFS 61
           S ++A+L DA+EKQ + + + +WL++L    Y+V+D +D+ K E  + K   + VL  + 
Sbjct: 39  SMIQAVLEDAQEKQLKYKAIKNWLQKLNVAAYEVDDILDDCKTEAARFK---QAVLGRYH 98

Query: 62  CSSRISANKLKQNFKRNKVCDELNKIAANMYEFHLKVKHIGSISMETTHSFPSASEISTR 121
             +     K+ +  K  ++ ++L+ IA     FHL  + I               E    
Sbjct: 99  PRTITFCYKVGKRMK--EMMEKLDAIAEERRNFHLDERII---------------ERQAA 158

Query: 122 HLKPSWQLLYPLKHASRNYDEAYDGIL--NVFNKYTHGFFHIVGEAGIGKSTMARFLYNH 181
             +  + L  P  +     ++    IL  NV          I+G  G+GK+T+A+ ++N 
Sbjct: 159 RRQTGFVLTEPKVYGREKEEDEIVKILINNVSYSEEVPVLPILGMGGLGKTTLAQMVFND 218

Query: 182 ENVVDRYTRRYWVCVEEGFNTHRLMKEIYSHADNKETREDLTTEQLLSKFIRLQREETFL 241
           + + + +  + WVCV + F+  RL+K I    + K +  D+    L  K   L   + + 
Sbjct: 219 QRITEHFNLKIWVCVSDDFDEKRLIKAIVESIEGK-SLGDMDLAPLQKKLQELLNGKRYF 278

Query: 242 LVFQDLSITNLDKCSTSVLIKLLEMGHHDSKIIVTTQTEEI 281
           LV  D  + N D+     L  +L++G   + I++TT+ E+I
Sbjct: 279 LVLDD--VWNEDQEKWDNLRAVLKIGASGASILITTRLEKI 296


HSP 4 Score: 45.4 bits (106), Expect = 3.6e-03
Identity = 32/88 (36.36%), Postives = 46/88 (52.27%), Query Frame = 1

Query: 635 LKYLRYLDLHGNQKIKHLPDSICDLQSLQTLILDSCSALEDLPKD-IRNLISLRYLWVT- 694
           L+ L YL +   + +K LP S+  L +L+ L +  C ALE LP++ +  L SL  L+V  
Sbjct: 880 LENLIYLSVSFLENLKELPTSLASLNNLKCLDIRYCYALESLPEEGLEGLSSLTELFVEH 939

Query: 695 TNKLHLHKNGVGTMTSLRFLAIGGCKNL 721
            N L     G+  +T+L  L I GC  L
Sbjct: 940 CNMLKCLPEGLQHLTTLTSLKIRGCPQL 967

BLAST of MELO3C009695 vs. Swiss-Prot
Match: RGA4_SOLBU (Putative disease resistance protein RGA4 OS=Solanum bulbocastanum GN=RGA4 PE=2 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 7.1e-44
Identity = 120/339 (35.40%), Postives = 178/339 (52.51%), Query Frame = 1

Query: 388 FKLKKLSKQSSSLLFKEYAFRNSPEVQNPELTKIVDQLLEKCMGVPLAIKCLGSLLSSKT 447
           + L  LS   S LLF + AF    E  NP L  I  ++++KC GVPLA K LG LL  K 
Sbjct: 308 YHLSNLSPHDSLLLFMQRAFGQQKEA-NPNLVAIGKEIVKKCGGVPLAAKTLGGLLRFKR 367

Query: 448 SIAEWKNI-EKKLMPQEKKEDGILHVLRVCYDQMPSQLKPCFLYCSQLPNDRIFSSNDMI 507
             +EW+++ + ++    + E  IL  LR+ Y  +P  L+ CF YC+  P D      ++I
Sbjct: 368 EESEWEHVRDNEIWSLPQDESSILPALRLSYHHLPLDLRQCFAYCAVFPKDTKMIKENLI 427

Query: 508 QLWMANGLLHSPEEKNLAMENIGKKYFMELWSRCFIQEIEEHGLGYWIKLHPLIQKLAHK 567
            LWMA+G L S  + NL +E++G + + EL+ R F QEIE      + K+H LI  LA  
Sbjct: 428 TLWMAHGFLLS--KGNLELEDVGNEVWNELYLRSFFQEIEAKSGNTYFKIHDLIHDLATS 487

Query: 568 I-TQEQSEGSWGNNHSKEITEIRSIAFQERNMVLPNASLTEKCIWRYKCLRLLYLGNADL 627
           + +   S G+    + K+     SI F         +S +   + ++  LR+L L  + L
Sbjct: 488 LFSASASCGNIREINVKDYKHTVSIGF-----AAVVSSYSPSLLKKFVSLRVLNLSYSKL 547

Query: 628 QEIPNSIGTLKYLRYLDLHGNQKIKHLPDSICDLQSLQTLILDSCSALEDLPKDIRNLIS 687
           +++P+SIG L +LRYLDL  N   + LP+ +C LQ+LQTL + +C +L  LPK    L S
Sbjct: 548 EQLPSSIGDLLHLRYLDLSCNN-FRSLPERLCKLQNLQTLDVHNCYSLNCLPKQTSKLSS 607

Query: 688 LRYLWVTTNKLHLHKNGVGTMTSLR---FLAIGGCKNLE 722
           LR+L V    L      +G +T L+   F  +G  K  +
Sbjct: 608 LRHLVVDGCPLTSTPPRIGLLTCLKTLGFFIVGSKKGYQ 637


HSP 2 Score: 87.4 bits (215), Expect = 8.2e-16
Identity = 73/282 (25.89%), Postives = 136/282 (48.23%), Query Frame = 1

Query: 2   SSLKAILLDAEEKQEQNQRLHDWLKELQNVFYQVEDSIDEFKWEFFKQKDTGKQVLAHFS 61
           S+++A+L DA+EKQ +++ + +WL++L +  Y+V+D + E K E  + + +    L  + 
Sbjct: 39  STIQAVLQDAQEKQLKDKAIENWLQKLNSAAYEVDDILGECKNEAIRFEQSR---LGFYH 98

Query: 62  CSSRISANKLKQNFKRNKVCDELNKIAANMYEFHLKVKHIGSISMETTHSFPSASEISTR 121
                  +K+ +  K  ++ ++L+ I+    +FH   K         T    +A+   T 
Sbjct: 99  PGIINFRHKIGRRMK--EIMEKLDAISEERRKFHFLEK--------ITERQAAAATRET- 158

Query: 122 HLKPSWQLLYPLKHA-SRNYDEAYDGILNVFNKYTH-GFFHIVGEAGIGKSTMARFLYNH 181
                + L  P  +   +  DE    ++N  N       F I+G  G+GK+T+A+ ++N 
Sbjct: 159 ----GFVLTEPKVYGRDKEEDEIVKILINNVNVAEELPVFPIIGMGGLGKTTLAQMIFND 218

Query: 182 ENVVDRYTRRYWVCVEEGFNTHRLMKEIYSHADNKETR-EDLTTEQLLSKFIRLQREETF 241
           E V   +  + WVCV + F+  RL+K I  + +      EDL + Q   K   L   + +
Sbjct: 219 ERVTKHFNPKIWVCVSDDFDEKRLIKTIIGNIERSSPHVEDLASFQ--KKLQELLNGKRY 278

Query: 242 LLVFQDLSITNLDKCSTSVLIKLLEMGHHDSKIIVTTQTEEI 281
           LLV  D+   +L+K +   L  +L +G   + I+ TT+ E++
Sbjct: 279 LLVLDDVWNDDLEKWAK--LRAVLTVGARGASILATTRLEKV 298


HSP 3 Score: 53.5 bits (127), Expect = 1.3e-05
Identity = 30/89 (33.71%), Postives = 49/89 (55.06%), Query Frame = 1

Query: 677 PKDIRNLISLRYLWVTTNKLHLHKNGVGTMTSLRFLAIGGCKNLENLFERPDCLAGLETL 736
           P  ++  +SLR L ++ +KL    + +G +  LR+L +  C N  +L ER   L  L+TL
Sbjct: 520 PSLLKKFVSLRVLNLSYSKLEQLPSSIGDLLHLRYLDLS-CNNFRSLPERLCKLQNLQTL 579

Query: 737 MIYNCNTLKLLPNEMRYLKSLQNLMIWRC 766
            ++NC +L  LP +   L SL++L++  C
Sbjct: 580 DVHNCYSLNCLPKQTSKLSSLRHLVVDGC 607


HSP 4 Score: 47.8 bits (112), Expect = 7.2e-04
Identity = 31/98 (31.63%), Postives = 51/98 (52.04%), Query Frame = 1

Query: 626 QEIPNSIGTLKYLRYLDLHGNQKIKHLPDSICDLQSLQTLILDSCSALEDLPKD-IRNLI 685
           +E+  S+  L++L + D    + +K LP S+  L +L+ L ++SC +LE  P+  +  L 
Sbjct: 871 EEMFTSLTNLEFLSFFDF---KNLKDLPTSLTSLNALKRLQIESCDSLESFPEQGLEGLT 930

Query: 686 SLRYLWVTTNK-LHLHKNGVGTMTSLRFLAIGGCKNLE 722
           SL  L+V   K L     G+  +T+L  L + GC  +E
Sbjct: 931 SLTQLFVKYCKMLKCLPEGLQHLTALTNLGVSGCPEVE 965


HSP 5 Score: 45.8 bits (107), Expect = 2.7e-03
Identity = 36/113 (31.86%), Postives = 51/113 (45.13%), Query Frame = 1

Query: 653 PDSICDLQSLQTLILDSCSALEDLPKDIRNLISLRYLWVTTNKLHLHKNGVGTMTSLRFL 712
           P  +    SL+ L L S S LE LP  I +L+ LRYL ++                    
Sbjct: 520 PSLLKKFVSLRVLNL-SYSKLEQLPSSIGDLLHLRYLDLS-------------------- 579

Query: 713 AIGGCKNLENLFERPDCLAGLETLMIYNCNTLKLLPNEMRYLKSLQNLMIWRC 766
               C N  +L ER   L  L+TL ++NC +L  LP +   L SL++L++  C
Sbjct: 580 ----CNNFRSLPERLCKLQNLQTLDVHNCYSLNCLPKQTSKLSSLRHLVVDGC 607

BLAST of MELO3C009695 vs. Swiss-Prot
Match: RGA2_SOLBU (Disease resistance protein RGA2 OS=Solanum bulbocastanum GN=RGA2 PE=1 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 7.4e-41
Identity = 119/332 (35.84%), Postives = 176/332 (53.01%), Query Frame = 1

Query: 388 FKLKKLSKQSSSLLFKEYAFRNSPEVQNPELTKIVDQLLEKCMGVPLAIKCLGSLLSSKT 447
           ++L  LS++   LLF + AF +  E+ NP L  I  ++++K  GVPLA K LG +L  K 
Sbjct: 307 YELSNLSQEDCWLLFMQRAFGHQEEI-NPNLVAIGKEIVKKSGGVPLAAKTLGGILCFKR 366

Query: 448 SIAEWKNIEKKL---MPQEKKEDGILHVLRVCYDQMPSQLKPCFLYCSQLPNDRIFSSND 507
               W+++       +PQ+  E  IL  LR+ Y Q+P  LK CF YC+  P D       
Sbjct: 367 EERAWEHVRDSPIWNLPQD--ESSILPALRLSYHQLPLDLKQCFAYCAVFPKDAKMEKEK 426

Query: 508 MIQLWMANGLLHSPEEKNLAMENIGKKYFMELWSRCFIQEIEEHGLGYWIKLHPLIQKLA 567
           +I LWMA+G L S  + N+ +E++G + + EL+ R F QEIE      + K+H LI  LA
Sbjct: 427 LISLWMAHGFLLS--KGNMELEDVGDEVWKELYLRSFFQEIEVKDGKTYFKMHDLIHDLA 486

Query: 568 HKITQEQSEGS---WGNNHSKEITEIRSIAFQERNMVLPNASLTEKCIWRYKCLRLLYLG 627
             +    +  S     N HS   T + SI F E         L      ++  LR+L LG
Sbjct: 487 TSLFSANTSSSNIREINKHS--YTHMMSIGFAEVVFFYTLPPLE-----KFISLRVLNLG 546

Query: 628 NADLQEIPNSIGTLKYLRYLDLHGNQKIKHLPDSICDLQSLQTLILDSCSALEDLPKDIR 687
           ++   ++P+SIG L +LRYL+L+G+  ++ LP  +C LQ+LQTL L  C+ L  LPK+  
Sbjct: 547 DSTFNKLPSSIGDLVHLRYLNLYGS-GMRSLPKQLCKLQNLQTLDLQYCTKLCCLPKETS 606

Query: 688 NLISLRYLWVT-TNKLHLHKNGVGTMTSLRFL 713
            L SLR L +  +  L      +G++T L+ L
Sbjct: 607 KLGSLRNLLLDGSQSLTCMPPRIGSLTCLKTL 625


HSP 2 Score: 98.2 bits (243), Expect = 4.6e-19
Identity = 71/283 (25.09%), Postives = 134/283 (47.35%), Query Frame = 1

Query: 2   SSLKAILLDAEEKQEQNQRLHDWLKELQNVFYQVEDSIDEFKWEF--FKQKDTGKQVLAH 61
           S+++A+L DA+EKQ  N+ L +WL++L    Y+V+D +DE+K +   F Q + G+     
Sbjct: 39  STIQAVLEDAQEKQLNNKPLENWLQKLNAATYEVDDILDEYKTKATRFSQSEYGRY---- 98

Query: 62  FSCSSRISANKLKQNFKRNKVCDELNKIAANMYEFHLKVKHIGSISM-ETTHSFPSASEI 121
                ++   + K   + ++V  +L  IA     FHL  K +   ++   T S  +  ++
Sbjct: 99  ---HPKVIPFRHKVGKRMDQVMKKLKAIAEERKNFHLHEKIVERQAVRRETGSVLTEPQV 158

Query: 122 STRHLKPSWQLLYPLKHASRNYDEAYDGILNVFNKYTH-GFFHIVGEAGIGKSTMARFLY 181
             R                +  DE    ++N  +   H     I+G  G+GK+T+A+ ++
Sbjct: 159 YGR---------------DKEKDEIVKILINNVSDAQHLSVLPILGMGGLGKTTLAQMVF 218

Query: 182 NHENVVDRYTRRYWVCVEEGFNTHRLMKEIYSHADNKETREDLTTEQLLSKFIRLQREET 241
           N + V + +  + W+CV E F+  RL+K I    + +    ++    L  K   L   + 
Sbjct: 219 NDQRVTEHFHSKIWICVSEDFDEKRLIKAIVESIEGRPLLGEMDLAPLQKKLQELLNGKR 278

Query: 242 FLLVFQDLSITNLDKCSTSVLIKLLEMGHHDSKIIVTTQTEEI 281
           +LLV  D  + N D+   + L  +L++G   + ++ TT+ E++
Sbjct: 279 YLLVLDD--VWNEDQQKWANLRAVLKVGASGASVLTTTRLEKV 297


HSP 3 Score: 48.1 bits (113), Expect = 5.5e-04
Identity = 32/88 (36.36%), Postives = 46/88 (52.27%), Query Frame = 1

Query: 635 LKYLRYLDLHGNQKIKHLPDSICDLQSLQTLILDSCSALEDLPKD-IRNLISLRYLWVT- 694
           L  L+YL +     +K LP S+  L +L++L +  C ALE LP++ +  L SL  L+V  
Sbjct: 858 LANLKYLTISRCNNLKELPTSLASLNALKSLKIQLCCALESLPEEGLEGLSSLTELFVEH 917

Query: 695 TNKLHLHKNGVGTMTSLRFLAIGGCKNL 721
            N L     G+  +T+L  L I GC  L
Sbjct: 918 CNMLKCLPEGLQHLTTLTSLKIRGCPQL 945


HSP 4 Score: 43.1 bits (100), Expect = 1.8e-02
Identity = 44/156 (28.21%), Postives = 67/156 (42.95%), Query Frame = 1

Query: 662 LQTLILDSCSALEDLPKDIRNLISLRYLW---VTTNKLHLHKNGVGTMTSLRFLAIGGCK 721
           L+ +I+  C  L  L  ++R L SLR  +    T+    + KN    + +L++L I  C 
Sbjct: 816 LEEMIIHECPFLT-LSSNLRALTSLRICYNKVATSFPEEMFKN----LANLKYLTISRCN 875

Query: 722 NLENLFERPDCLAGLETLMIYNCNTLKLLPNE-MRYLKSLQNLMIWRCKQLTLNLEEVEF 781
           NL+ L      L  L++L I  C  L+ LP E +  L SL  L +  C  L         
Sbjct: 876 NLKELPTSLASLNALKSLKIQLCCALESLPEEGLEGLSSLTELFVEHCNML--------- 935

Query: 782 KLQRFTVKELPKVESLPRWLENSIETLRTLQIINCP 814
                        + LP  L++ + TL +L+I  CP
Sbjct: 936 -------------KCLPEGLQH-LTTLTSLKIRGCP 943

BLAST of MELO3C009695 vs. Swiss-Prot
Match: R13L1_ARATH (Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana GN=RPPL1 PE=3 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 3.0e-34
Identity = 108/358 (30.17%), Postives = 173/358 (48.32%), Query Frame = 1

Query: 387 IFKLKKLSKQSSSLLFKEYAFRNSPEVQNPELTKIVDQLLEKCMGVPLAIKCLGSLLSSK 446
           +  L+ LS      LF +  F N     N E+  + ++++ KC G+PLA+K LG +L  +
Sbjct: 328 VHNLQPLSDGDCWSLFMKTVFGNQEPCLNREIGDLAERIVHKCRGLPLAVKTLGGVLRFE 387

Query: 447 TSIAEWKNI-EKKLMPQEKKEDGILHVLRVCYDQMPSQLKPCFLYCSQLPNDRIFSSNDM 506
             + EW+ +   ++      +  +L VLRV Y  +P+ LK CF YCS  P    F  + +
Sbjct: 388 GKVIEWERVLSSRIWDLPADKSNLLPVLRVSYYYLPAHLKRCFAYCSIFPKGHAFEKDKV 447

Query: 507 IQLWMANGLLHSPEE-KNLAMENIGKKYFMELWSRCFIQEIEEHGLGYWIKLHPLIQKLA 566
           + LWMA G L      KNL  E +G +YF EL SR  +Q+ +   +     +H  I +LA
Sbjct: 448 VLLWMAEGFLQQTRSSKNL--EELGNEYFSELESRSLLQKTKTRYI-----MHDFINELA 507

Query: 567 HKITQEQSE-----------------GSWGNNHSK--EITEIRSIAFQER--NMVLPNAS 626
              + E S                      +N+++  E   +R + F      + L N+S
Sbjct: 508 QFASGEFSSKFEDGCKLQVSERTRYLSYLRDNYAEPMEFEALREVKFLRTFLPLSLTNSS 567

Query: 627 --------LTEKCIWRYKCLRLLYLGNADLQEIP-NSIGTLKYLRYLDLHGNQKIKHLPD 686
                   ++EK +     LR+L L +  +  +P +    + + R+LDL    +++ LP 
Sbjct: 568 RSCCLDQMVSEKLLPTLTRLRVLSLSHYKIARLPPDFFKNISHARFLDL-SRTELEKLPK 627

Query: 687 SICDLQSLQTLILDSCSALEDLPKDIRNLISLRYLWVTTNKLHLHKNGVGTMTSLRFL 713
           S+C + +LQTL+L  CS+L++LP DI NLI+LRYL +   KL       G + SL+ L
Sbjct: 628 SLCYMYNLQTLLLSYCSSLKELPTDISNLINLRYLDLIGTKLRQMPRRFGRLKSLQTL 677


HSP 2 Score: 74.3 bits (181), Expect = 7.2e-12
Identity = 73/293 (24.91%), Postives = 125/293 (42.66%), Query Frame = 1

Query: 3   SLKAILLDAEEKQEQNQRLHDWLKELQNVFYQVEDSIDEFKWEFFK--------QKDTGK 62
           ++ A+L+DAEEKQ  N  +  W+ EL++V Y  ED++D+   E  +          +  +
Sbjct: 48  TITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALRLNIGAESSSSNRLR 107

Query: 63  QVLAHFSCSSRISANKLKQNFKRNKVCDELNKIAANMYEFHLKVKHIGSISMETTHSFPS 122
           Q+    S    +  N      +  KV   L ++A+      LK         E T   P 
Sbjct: 108 QLRGRMSLGDFLDGNSEHLETRLEKVTIRLERLASQRNILGLK---------ELTAMIP- 167

Query: 123 ASEISTRHLKPSWQLLYPLKHASRNYDEAYDGILNVF---NKYTHGF--FHIVGEAGIGK 182
                 +   P+  L+   +   R+ D+  D I+      N   +G     IVG  G+GK
Sbjct: 168 ------KQRLPTTSLVDESEVFGRDDDK--DEIMRFLIPENGKDNGITVVAIVGIGGVGK 227

Query: 183 STMARFLYNHENVVDRYTRRYWVCVEEGFNTHRLMKEIYSHADNKETR-EDLTTEQLLSK 242
           +T+++ LYN ++V   +  + W  V E F+  ++ K++Y    ++     DL   Q+  K
Sbjct: 228 TTLSQLLYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVTSRPCEFTDLDVLQVKLK 287

Query: 243 FIRLQREETFLLVFQDLSITNLDKCSTSVLIKLLEMGHHDSKIIVTTQTEEIA 282
                    FLLV  DL   N +     +L +        S+I+VTT+++ +A
Sbjct: 288 ERLTGTGLPFLLVLDDL--WNENFADWDLLRQPFIHAAQGSQILVTTRSQRVA 320

BLAST of MELO3C009695 vs. TrEMBL
Match: A0A0A0LBH9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G815400 PE=3 SV=1)

HSP 1 Score: 1371.3 bits (3548), Expect = 0.0e+00
Identity = 704/875 (80.46%), Postives = 749/875 (85.60%), Query Frame = 1

Query: 1   MSSLKAILLDAEEKQEQNQRLHDWLKELQNVFYQVEDSIDEFKWEFFKQKDTGKQVLAHF 60
           MSSLKA+LLDAEEKQEQNQRL +WLKELQNVFYQVEDSIDEFKWE FKQKD GKQVLA F
Sbjct: 30  MSSLKAVLLDAEEKQEQNQRLQNWLKELQNVFYQVEDSIDEFKWEIFKQKDIGKQVLAPF 89

Query: 61  SCSSRISANKLKQNFKRNKVCDELNKIAANMYEFHLKVKHIGSISMETTHSFPSASEIST 120
           SCS++ISANKLKQ  KR +VCDELN IA  MYEFHLKVKHI SISMETTH+FPSASEIS 
Sbjct: 90  SCSNQISANKLKQKCKRKQVCDELNNIATRMYEFHLKVKHIDSISMETTHTFPSASEISI 149

Query: 121 RHLKPSWQLLYPLKHASRNYDEAYDGILNVFNKYTHGFFHIVGEAGIGKSTMARFLYNHE 180
           RHLKPSWQLLYPL  ASR YDE YDGILNVFN+ TH  FHIVGEAGIGKST+ARFLYNH 
Sbjct: 150 RHLKPSWQLLYPLIDASRTYDEIYDGILNVFNECTH-VFHIVGEAGIGKSTVARFLYNHN 209

Query: 181 NVVDRYTRRYWVCVEEGFNTHRLMKEIYSHADNKETREDLTTEQLLSKFIRLQREETFLL 240
           NVV +YT RYWVCVEEGFN HRL+KE+YSHADNKE  EDLTTEQLLSK  RL + ETFLL
Sbjct: 210 NVVGKYTSRYWVCVEEGFNKHRLVKEVYSHADNKEICEDLTTEQLLSKVKRLLKVETFLL 269

Query: 241 VFQDLSITNLDKCSTSVLIKLLEMGHHDSKIIVTTQTEEIAKDIDVHGYKTEIQSKKNLA 300
           VFQDLSITNL   S  VL +LLEMG H SKIIVTTQTEEIA  +   GYKTE +SK+NLA
Sbjct: 270 VFQDLSITNLKDRSPLVLNELLEMGQHGSKIIVTTQTEEIANYLQDRGYKTERRSKENLA 329

Query: 301 NGDEVSADKQSLTKNTQNQAVPGSQTEETAGAIANMIYETEKNLEKNPVAGDGESESSRL 360
           NGD VSA  QSLTKNTQNQAVPGSQ EETA AIAN IYE   +L                
Sbjct: 330 NGDRVSAHNQSLTKNTQNQAVPGSQIEETADAIANKIYEHSLSL---------------- 389

Query: 361 ITKDAQNLDVSIPQVTEC---------------EPDRGYQTIFKLKKLSKQSSSLLFKEY 420
            T+DAQNL+V IPQVTE                + D  YQTIFKL+KLSKQSSS LFKEY
Sbjct: 390 -TEDAQNLEVPIPQVTEDTNIGLGINPDIPPIKQDDTEYQTIFKLEKLSKQSSSRLFKEY 449

Query: 421 AFRNSPEVQNPELTKIVDQLLEKCMGVPLAIKCLGSLLSSKTSIAEWKNIEKKLMPQEKK 480
           AFRN  EV+NPELTKIVDQLLEKCMGVPLAIKCLGSLLSS+TSIA+WKNIE+KL  QEKK
Sbjct: 450 AFRNRQEVENPELTKIVDQLLEKCMGVPLAIKCLGSLLSSETSIAKWKNIEEKLSLQEKK 509

Query: 481 EDGILHVLRVCYDQMPSQLKPCFLYCSQLPNDRIFSSNDMIQLWMANGLLHSPEEKNLAM 540
           E+GILHVLRVCYDQMPSQLKPCFL+CSQLPNDRI SSNDMIQLWMANGLLHSPEEKN  M
Sbjct: 510 ENGILHVLRVCYDQMPSQLKPCFLHCSQLPNDRIISSNDMIQLWMANGLLHSPEEKNSTM 569

Query: 541 ENIGKKYFMELWSRCFIQEIEEHGLGYWIKLHPLIQKLAHKITQEQSEGSWGNNHSKEIT 600
           ENIG+KYFMELWSR FIQEIEEHGLGYWIKLHPLIQKLAHKITQEQSEGS GNNH KE+T
Sbjct: 570 ENIGEKYFMELWSRYFIQEIEEHGLGYWIKLHPLIQKLAHKITQEQSEGSGGNNHPKEVT 629

Query: 601 EIRSIAFQERNMVLPNASLTEKCIWRYKCLRLLYLGNADLQEIPNSIGTLKYLRYLDLHG 660
           EIRSIAFQERNMVLPNASLTEKCIW+YK LRLLYL NADLQEIPNSIGTLKYLRYLDLHG
Sbjct: 630 EIRSIAFQERNMVLPNASLTEKCIWKYKGLRLLYLSNADLQEIPNSIGTLKYLRYLDLHG 689

Query: 661 NQKIKHLPDSICDLQSLQTLILDSCSALEDLPKDIRNLISLRYLWVTTNKLHLHKNGVGT 720
           N KIKHLP+SIC+LQSLQTLIL SCSALEDLPKDIRNLISLRYLWVTTNKL L KNGVGT
Sbjct: 690 NTKIKHLPNSICNLQSLQTLILGSCSALEDLPKDIRNLISLRYLWVTTNKLRLDKNGVGT 749

Query: 721 MTSLRFLAIGGCKNLENLFERPDCLAGLETLMIYNCNTLKLLPNEMRYLKSLQNLMIWRC 780
           M SLRFLAIGGC NLENLFERPDCLAGLETLMIYNC TLKLLP+EMRYLKSLQNLMIW C
Sbjct: 750 MNSLRFLAIGGCNNLENLFERPDCLAGLETLMIYNCTTLKLLPDEMRYLKSLQNLMIWSC 809

Query: 781 KQLTLNLEEVEFKLQRFTVKELPKVESLPRWLENSIETLRTLQIINCPIRLMELPVDKKY 840
           KQLTLNL+EVEFKLQRFT+KELP+VE LP+WLENS ETLRTLQIINCPIR+ME    +KY
Sbjct: 810 KQLTLNLKEVEFKLQRFTIKELPRVERLPQWLENSAETLRTLQIINCPIRIMERQGIEKY 869

Query: 841 RALEIFLIHGSVRFD-TMPDYDFEHRNLTIFRGNE 860
            A+E  +I+G+VRF+   P YDFEHRNL +  GNE
Sbjct: 870 EAVENTIIYGAVRFEMAPPGYDFEHRNLAVRNGNE 886

BLAST of MELO3C009695 vs. TrEMBL
Match: A0A0A0LF99_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G814390 PE=3 SV=1)

HSP 1 Score: 679.5 bits (1752), Expect = 5.4e-192
Identity = 408/859 (47.50%), Postives = 540/859 (62.86%), Query Frame = 1

Query: 1   MSSLKAILLDAEEKQEQNQRLHDWLKELQNVFYQVEDSIDEFKWEFFKQKDTGKQV-LAH 60
           M SLK IL DAE K+E    L+DWL++LQ+VF Q+E+ + E   E  KQ+ TGK V L  
Sbjct: 30  MLSLKTILKDAE-KEEYRHCLNDWLQKLQSVFLQIEELLYESNREVKKQEATGKWVFLPS 89

Query: 61  FSCSSRISANKLKQNFKRNKVCDELNKIAANMYEFHLKVKHIGSISMETTHSFPSASEIS 120
           F+ S      ++ Q  K  K+CD+L++IA++MY F+L        +METTHSF SA+E+S
Sbjct: 90  FNFS------QIDQTKKMMKLCDDLDEIASHMYGFNLT-------NMETTHSFLSATEVS 149

Query: 121 TRHLKPSWQLLYPLKHASRNY-DEAYDGILNVFNKYTHGFFHIVGEAGIGKSTMARFLYN 180
           TR +KPSWQLLY L +A + + D+ Y   L+ F K THG FHIVGE GIGK+T+A+F YN
Sbjct: 150 TRLMKPSWQLLYSLTNAPKVFQDKRYHNFLDHFKKSTHGLFHIVGEPGIGKTTLAKFFYN 209

Query: 181 HENVVDRYTRRYWVCVEEGFNTHRLMKEIYSHADNKETREDLTTEQLLSKFIRLQREETF 240
             N+V+ +  R W+CV+E F+                                 QR    
Sbjct: 210 --NLVNTFPSRLWICVKEEFDP--------------------------------QRLIKE 269

Query: 241 LLVFQDLSITNLDKCSTSVLIKLLEMGHHDSKIIVTTQTEEIAKDIDVHGYKTEIQSKKN 300
           +L F    +T  D  +   L   ++    D K ++  Q      DI +          KN
Sbjct: 270 MLSFSHCQVT-CDNLTEKQLCFAVQQFLRDKKFLIVFQ------DISI----------KN 329

Query: 301 LANGDEVSADKQSLTK-NTQNQAVPGSQTEETAGAIA-NMIYETEKNLEKNPVAGDGESE 360
           L N    S  K  L   N  ++ +  +Q E+ A A+    +Y+ E  +  +P     E+ 
Sbjct: 330 LGN---CSIFKSLLGMGNRGSKIIVTTQNEKIADAVGLKKLYKNESQVVPSP-----EAT 389

Query: 361 SSRLITKDAQNLDVSIPQVTECEPDRGYQTIFKLKKLSKQSSSLLFKEYAFRNSPEVQNP 420
               + KD                   +QTIFK+++LSK++S  LFK +AF  + E Q P
Sbjct: 390 KPSDVNKDNMK----------------HQTIFKVERLSKENSLSLFKVHAFTETQEAQIP 449

Query: 421 ELTKIVDQLLEKCMGVPLAIKCLGSLLSSKTSIAEWKNIEKKLMPQEKKEDG---ILHVL 480
            LTKI + + +KC GVPLAIKCLG LLS KTSIAEW  +  KL   E++EDG   IL  L
Sbjct: 450 NLTKIQEVIEQKCHGVPLAIKCLGGLLS-KTSIAEWNGVIDKLWEHEEEEDGNKSILPTL 509

Query: 481 RVCYDQMPSQLKPCFLYCSQLPNDRIFSSNDMIQLWMANGLLHSPEEKNLAMENIGKKYF 540
           R+CYDQMPS L+ CFLYCSQL  DRI SSND+IQLW+A+ LL  P+E  L++E IG+ YF
Sbjct: 510 RLCYDQMPSHLQRCFLYCSQLKKDRILSSNDVIQLWIASDLL--PKENYLSLEKIGENYF 569

Query: 541 MELWSRCFIQEIEEHGLGYWIKLHPLIQKLAHKITQEQSEGSWGNNHSKEITEIRSIAFQ 600
            EL SRCF+QE+EE+G GYW KLHPLI+KLA  +TQ+Q           E+T+ +SIAF 
Sbjct: 570 KELCSRCFLQELEEYGFGYWFKLHPLIEKLARLLTQKQVF---------EVTKTQSIAFT 629

Query: 601 ERNMVLPNASLTEKCIWRYKCLRLLYLGNADLQEIPNSIGTLKYLRYLDLHGNQKIKHLP 660
            R+ V P+A L   CI ++K LRLL+LGNA+LQ IP+++  L  LRYLDL GN+KIK LP
Sbjct: 630 IRDKVPPSAFLANACIDKFKYLRLLHLGNANLQGIPSAVENLVQLRYLDLQGNKKIKRLP 689

Query: 661 DSICDLQSLQTLILDSCSALEDLPKDIRNLISLRYLWVTTNKLHLHKNGVGTMTSLRFLA 720
           +SI  L++LQTLIL SCSAL++LP DIR L +LRYLWVT N L LHKNGVGTMTSLRFLA
Sbjct: 690 NSIFKLKNLQTLILASCSALKELPNDIRQLTNLRYLWVTANNLRLHKNGVGTMTSLRFLA 749

Query: 721 IGGCKNLENLFERPDCLAGLETLMIYNCNTLKLLPNEMRYLKSLQNLMIWRCKQLTLNLE 780
           IGGC+NL++LF++P CL  LETLMIY+C TLK LPNE+  L SL+NL+IW CK+LTL L+
Sbjct: 750 IGGCQNLQDLFKKPSCLVRLETLMIYDCKTLKSLPNEIGSLISLKNLVIWSCKKLTLTLK 786

Query: 781 EVEFKLQRFTVKELPKVESLPRWLENSIETLRTLQIINCPIRLMELPVDKKYRALEIFLI 840
            VEF+LQRFT++ELP V+ LP W +   ETLR L+II+CPI   +  V K Y++LE F I
Sbjct: 810 GVEFRLQRFTIRELPIVKKLPEWTQRFTETLRVLEIIDCPIEWND-DVLKSYKSLERFSI 786

Query: 841 HGSVRF-DTMPDYDFEHRN 852
           HG+VR  + +  Y+ ++RN
Sbjct: 870 HGAVRTKNQIGGYNIDYRN 786

BLAST of MELO3C009695 vs. TrEMBL
Match: A0A0A0LEM8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G814400 PE=3 SV=1)

HSP 1 Score: 516.2 bits (1328), Expect = 8.0e-143
Identity = 286/544 (52.57%), Postives = 378/544 (69.49%), Query Frame = 1

Query: 308 DKQSLTKNTQNQAVPGS------QTEETAGAIA-NMIYETEKNLEKNPVAGDGESESSRL 367
           D  SL  +      PGS      Q EE A AI   MIY+  +  E+N         +   
Sbjct: 273 DNCSLFTSLLMMGKPGSKIIVTTQNEEIANAIELTMIYKVGQQSEQNR-----SQTALDT 332

Query: 368 ITKDAQNLDVSIPQVTECEP----DRGY--QTIFKLKKLSKQSSSLLFKEYAFRNSPEVQ 427
           +TK+  N++ +  Q  +  P    D+    QTIFK+K+LS++ S  LFK+YA  ++ E  
Sbjct: 333 VTKETANVN-NADQFVQANPLGKIDQSIPSQTIFKVKRLSEKDSLSLFKDYA--STYEGN 392

Query: 428 NPELTKIVDQLLEKCMGVPLAIKCLGSLLSSKTSIAEWKNIEKKLMPQEKKEDGILHVLR 487
             ++ K     L+KC G+PLAIKCLGS+LS      +W   + +    + +      +L+
Sbjct: 393 EKDIMKT----LKKCNGIPLAIKCLGSMLSLGPPATKWME-DNERQKGDNESSSTFSILK 452

Query: 488 VCYDQMPSQLKPCFLYCSQLPNDRIFSSNDMIQLWMANGLLHSPEEKNLAMENIGKKYFM 547
           +CY++MPS LK CFLYCSQLPND I SSND+IQLWMANGLL S +E  L++E+IG+ YF 
Sbjct: 453 LCYNEMPSHLKRCFLYCSQLPNDSILSSNDVIQLWMANGLLRSRQENYLSLEDIGEIYFK 512

Query: 548 ELWSRCFIQEIEEHGLGYWIKLHPLIQKLAHKITQEQSEGSWGNNHSKEITEIRSIAFQE 607
           EL SRCF+Q++EE+GLGYW K+HPLI++LA ++ Q++++        K +T + SIAF  
Sbjct: 513 ELCSRCFLQDVEEYGLGYWFKMHPLIRELA-RLVQKRTKDLIS---IKPVTNVTSIAFPV 572

Query: 608 RNMVLPNASLTEKCIWRYKCLRLLYLGNADLQEIPNSIGTLKYLRYLDLHGNQKIKHLPD 667
           R+ V  ++ L EKCI +++ LRLLYLG+ DLQEIPN+I TL +L YLDL GN+ IK LP+
Sbjct: 573 RDEVPSSSFLAEKCISKFQHLRLLYLGHTDLQEIPNTIETLNHLTYLDLQGNKNIKRLPN 632

Query: 668 SICDLQSLQTLILDSCSALEDLPKDIRNLISLRYLWVTTNKLHLHKNGVGTMTSLRFLAI 727
           +IC+LQ LQTLIL SCSALE+LPKDI  L +LRYLWVT+NKL LHKNGVGTMTSLRFLAI
Sbjct: 633 AICNLQHLQTLILASCSALEELPKDICKLSNLRYLWVTSNKLRLHKNGVGTMTSLRFLAI 692

Query: 728 GGCKNLENLFERPDCLAGLETLMIYNCNTLKLLPNEMRYLKSLQNLMIWRCKQLTL-NLE 787
           GGC  L++LFERP CL  LETLMIY+CN+L+LLPNEM  L SLQNL+IW CKQLTL  LE
Sbjct: 693 GGCDKLQDLFERPSCLVRLETLMIYDCNSLQLLPNEMGSLISLQNLVIWSCKQLTLKGLE 752

Query: 788 EVEFKLQRFTVKELPKVESLPRWLENSIETLRTLQIINCPIRLMELPVDKKYRALEIFLI 838
           +V+F LQRFT++ELP+V  LP WL+ S ETLR L+II+CPI++ E  + K Y+A+E  +I
Sbjct: 753 KVDFSLQRFTIRELPEVNKLPEWLQRSTETLRVLEIIDCPIKVEEEGI-KMYKAVESKII 798

BLAST of MELO3C009695 vs. TrEMBL
Match: A0A0A0LEM8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G814400 PE=3 SV=1)

HSP 1 Score: 288.1 bits (736), Expect = 3.5e-74
Identity = 172/353 (48.73%), Postives = 234/353 (66.29%), Query Frame = 1

Query: 1   MSSLKAILLDAEEKQEQNQRLHDWLKELQNVFYQVEDSIDEFKWEFFKQKDTGKQVLAHF 60
           M SLK+IL+DAEEKQEQ++ L +WL+ELQNVF Q+E  IDE K E ++    GKQVLA F
Sbjct: 30  MLSLKSILMDAEEKQEQSRGLQNWLEELQNVFSQIEGFIDEHKEEAYE--GIGKQVLAPF 89

Query: 61  SCSSRISANKLKQNFKRNKVCDELNKIAANMYEFHLKVKHIGSISMETTHSFPSASEIST 120
           SCSS    N++ + +K  K+ D LN++AA MYEF+L  +H G+I  ETT+SF +A+E+ST
Sbjct: 90  SCSS----NQIARTWKMEKLFDHLNEVAAKMYEFNLTERHTGAIKTETTNSFLTATEVST 149

Query: 121 RHLKPSWQLLYPLKHASRNY-DEAYDGILNVFNKYTHGFFHIVGEAGIGKSTMARFLYNH 180
           R +KPSW++LYPL +A + Y DE Y  ILN F   T GFFHIVGEAGIGKST+A+F+YN 
Sbjct: 150 RLMKPSWKVLYPLTNAPKFYQDERYRKILNDFKNPTLGFFHIVGEAGIGKSTLAKFIYND 209

Query: 181 ENVVDRYTRRYWVCVEEGFNTHRLMKEIYSHADNKETREDLTTEQLLSKFIRLQREETFL 240
             V   +  R WVCV+E F+T RLMKEI + + +  T ++LTT+  L    +  RE TFL
Sbjct: 210 PEVEGMFPSRLWVCVKEEFDTQRLMKEILNFSYSPATCDNLTTK--LCPTDQYLRERTFL 269

Query: 241 LVFQDLSITNLDKCSTSVLIKLLEMGHHDSKIIVTTQTEEIAKDIDVHG-YKTEIQSKKN 300
           LVFQDLSI NLD C  S+   LL MG   SKIIVTTQ EEIA  I++   YK   QS++N
Sbjct: 270 LVFQDLSIKNLDNC--SLFTSLLMMGKPGSKIIVTTQNEEIANAIELTMIYKVGQQSEQN 329

Query: 301 LANGDEVSADKQSLTKNTQNQAVPGSQTEETAGAI-ANMIYETEKNLEKNPVA 351
            +     +  K++   N  +Q V  +   +   +I +  I++ ++  EK+ ++
Sbjct: 330 RSQTALDTVTKETANVNNADQFVQANPLGKIDQSIPSQTIFKVKRLSEKDSLS 372


HSP 2 Score: 325.9 bits (834), Expect = 1.5e-85
Identity = 196/456 (42.98%), Postives = 269/456 (58.99%), Query Frame = 1

Query: 386 TIFKLKKLSKQSSSLLFKE-YAFRNSPEVQNPELTKIVDQLLEKCMGVPLAIKCLGSLLS 445
           T  K+ KL +  S  +F++ Y      E    +L   +  L+ +C G PLAIKCL  LLS
Sbjct: 76  TTHKIDKLDETISMQIFEDTYGSEGLSEGLRDDL--YLKNLVAECGGAPLAIKCLAGLLS 135

Query: 446 SKTSIAEWKNIEKKLMPQEKKED-----GILHVLRVCYDQMPSQLKPCFLYCSQLPNDRI 505
           SK S        K L  + K+E+     G+L  LR+ YD MPS LKPCFL  S LP D +
Sbjct: 136 SKPSDGAKSPNVKDLSEKWKQEEANNGGGVLCALRLSYDLMPSYLKPCFLCFSVLPKDNV 195

Query: 506 FSSNDMIQLWMANGLLHSPEEKNLAMENIGKKYFMELWSRCFIQEIEEHGLGYWIKLHPL 565
           F S ++IQLWMA G+L S  + N   E +G+KYF E   R  + ++EEH LGYW K+H L
Sbjct: 196 FFSFELIQLWMAQGILPSGTKDN--PEEVGEKYFKEFRDRRLLVDVEEHTLGYWFKIHSL 255

Query: 566 IQKLAHKITQEQSEGSWGNNHSKEITEIRSIAFQE-----RNMVLP---------NASLT 625
           +  LA +   EQ   + GN H     +  SI         R + +P         N+ L 
Sbjct: 256 VHDLAVQKATEQK--NLGNFHMLSFVDCDSIPSSTNYDNTRFISIPVVGGAGPNINSDLF 315

Query: 626 EKCIWRYKCLRLLYLGNADLQEIPNSIGTLKYLRYLDLHGNQKIKHLPDSICDLQSLQTL 685
            KCI +++ LR LYL N+ L+EIP SI TLK+LR LDL G+Q++K LP+SIC LQSLQTL
Sbjct: 316 -KCITQFRQLRFLYLCNSSLEEIPTSIDTLKHLRCLDLRGSQRLKRLPESICKLQSLQTL 375

Query: 686 ILDSCSALEDLPKDIRNLISLRYLWVTTNKLHLHKNGVGTMTSLRFLAIGGCKNLENLFE 745
           +L  CS LE+LP++I+NLISLR+LW+ T +  L K+ +G++TSLRFLAIG  +NL +LFE
Sbjct: 376 VLAFCSELEELPRNIKNLISLRFLWIQTKQARLEKDEIGSLTSLRFLAIGRSENLTHLFE 435

Query: 746 RPDCLAGLETLMIYNCNTLKLLPNEMRYLKSLQNLMIWRCKQLTLNLEEVEFKLQRFTVK 805
             + L  L+TL+IY C +L  LP  +  +KS+ N+ IW C +L          L++  ++
Sbjct: 436 DINKLNSLKTLIIYECKSLLTLPKGLENMKSICNMGIWECDRLRFTFSLASLHLKKLILR 495

Query: 806 ELPKVESLPRWLENSIETLRTLQIINCPIRLMELPV 822
           EL  V +LP WL N   TL  L+I   P  L +LP+
Sbjct: 496 ELTAVSTLPNWLSNLDGTLEVLEIGEFP-TLRKLPI 523

BLAST of MELO3C009695 vs. TrEMBL
Match: A0A0A0LBX7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G822460 PE=3 SV=1)

HSP 1 Score: 37.0 bits (84), Expect = 1.4e+02
Identity = 26/77 (33.77%), Postives = 40/77 (51.95%), Query Frame = 1

Query: 216 TREDLTTEQLLSKFIRLQREETF-----LLVFQDLSITNLDKCSTSVLIKLLEMGHHDSK 275
           T E++TT  L    +R++ +  F     LLV  D    NLD    S L K+++MG   SK
Sbjct: 4   TGENVTTVALTESRLRIRLQRYFFGKKILLVLDDFG--NLDPERVSELKKIVKMGVGGSK 63

Query: 276 IIVTTQTEEIAKDIDVH 288
           I++TT+++E       H
Sbjct: 64  IMITTRSDETLNVATTH 78


HSP 2 Score: 310.1 bits (793), Expect = 8.7e-81
Identity = 186/461 (40.35%), Postives = 258/461 (55.97%), Query Frame = 1

Query: 390 LKKLSKQSSSLLFKEYAFRNSPEVQNPELTKIVDQLLEKCMGVPLAIKCLGSLLSSKTSI 449
           L  LS++ S  LF + AF    E   P+L  I  +++ KC G P+A+K LGSLL SKT+ 
Sbjct: 306 LANLSQEDSLTLFMKCAFEQGQERYFPDLMVIGKEIVNKCGGNPMAVKTLGSLLHSKTNQ 365

Query: 450 AEWKNIEKKLMPQEKKEDGILHVLRVCYDQMPSQLKPCFLYCSQLPNDRIFSSNDMIQLW 509
           + WK+I    + Q + +  IL  LR+ YD MPS LK CF YCS  P +  F++ ++IQLW
Sbjct: 366 SNWKSIRDSEIWQLQTD--ILPSLRISYDLMPSYLKQCFAYCSIFPKNYEFNNLELIQLW 425

Query: 510 MANGLLHSPEEKNLAMENIGKKYFMELWSRCFIQEIEEHGLGYWIKLHPLIQKLAHKITQ 569
           +ANGL+  P   N  +E IG++Y+ ELWSR F  ++ E  L    K+H LI+ LA  + Q
Sbjct: 426 IANGLIQ-PNRNNTELEEIGQQYWEELWSRSFFDDVVEDYLFLKFKMHDLIRALAISVAQ 485

Query: 570 EQSEG--------SWGNNHSK-----------------EITEIRSIAFQERNMVLPNASL 629
             S          S    H                   E++ IR+I F  +       S 
Sbjct: 486 NDSSNVNMRTQDVSLTTRHISFSDPSLLPEDELPGYLSELSGIRTIMFPIKKEGSTGESF 545

Query: 630 TEKCIWRYKCLRLLYLGNADLQEIPNSIGTLKYLRYLDLHGNQKIKHLPDSICDLQSLQT 689
            E CI R+K LR+LYL ++   ++PNSIG LK+LR+  L GN+ IK LP+SIC+L  LQ+
Sbjct: 546 LEMCISRFKHLRVLYLHDSSFDQLPNSIGGLKHLRFFHLCGNRNIKKLPNSICELHHLQS 605

Query: 690 LILDSCSALEDLPKDIRNLISLRYLWVTTNKLHLHKNGVGTMTSLRFLAIGGCKNLENLF 749
           L L  C  LE+LP DI+N+ISLR+L VTT +    ++G+G +TSLR L IG CKNLE LF
Sbjct: 606 LGLARCEELEELPADIKNMISLRFLDVTTKQRRFPESGIGCLTSLRCLFIGDCKNLEALF 665

Query: 750 ERPDCLAGLETLMIYNCNTLKLLPNEMRYLKSLQNLMIWRCKQLTL-----NLEEVEFKL 809
                L  L  L I  C  L  LP +++ LK+L+NL I  C+ L L     +      +L
Sbjct: 666 NDMQSLTSLRKLFIGGCPKLTSLPQDIKNLKALENLWICNCENLKLQEGGSDEPRSMSRL 725

Query: 810 QRFTVKELPKVESLPRWLENSIETLRTLQIINCPIRLMELP 821
           Q      LPK+ S PRWL+ S  T++ + + +CP  L  LP
Sbjct: 726 QSMMFVGLPKIVSFPRWLKGSARTMQAIDVAHCP-NLSTLP 762

BLAST of MELO3C009695 vs. TAIR10
Match: AT3G14470.1 (AT3G14470.1 NB-ARC domain-containing disease resistance protein)

HSP 1 Score: 148.7 bits (374), Expect = 1.7e-35
Identity = 108/358 (30.17%), Postives = 173/358 (48.32%), Query Frame = 1

Query: 387 IFKLKKLSKQSSSLLFKEYAFRNSPEVQNPELTKIVDQLLEKCMGVPLAIKCLGSLLSSK 446
           +  L+ LS      LF +  F N     N E+  + ++++ KC G+PLA+K LG +L  +
Sbjct: 328 VHNLQPLSDGDCWSLFMKTVFGNQEPCLNREIGDLAERIVHKCRGLPLAVKTLGGVLRFE 387

Query: 447 TSIAEWKNI-EKKLMPQEKKEDGILHVLRVCYDQMPSQLKPCFLYCSQLPNDRIFSSNDM 506
             + EW+ +   ++      +  +L VLRV Y  +P+ LK CF YCS  P    F  + +
Sbjct: 388 GKVIEWERVLSSRIWDLPADKSNLLPVLRVSYYYLPAHLKRCFAYCSIFPKGHAFEKDKV 447

Query: 507 IQLWMANGLLHSPEE-KNLAMENIGKKYFMELWSRCFIQEIEEHGLGYWIKLHPLIQKLA 566
           + LWMA G L      KNL  E +G +YF EL SR  +Q+ +   +     +H  I +LA
Sbjct: 448 VLLWMAEGFLQQTRSSKNL--EELGNEYFSELESRSLLQKTKTRYI-----MHDFINELA 507

Query: 567 HKITQEQSE-----------------GSWGNNHSK--EITEIRSIAFQER--NMVLPNAS 626
              + E S                      +N+++  E   +R + F      + L N+S
Sbjct: 508 QFASGEFSSKFEDGCKLQVSERTRYLSYLRDNYAEPMEFEALREVKFLRTFLPLSLTNSS 567

Query: 627 --------LTEKCIWRYKCLRLLYLGNADLQEIP-NSIGTLKYLRYLDLHGNQKIKHLPD 686
                   ++EK +     LR+L L +  +  +P +    + + R+LDL    +++ LP 
Sbjct: 568 RSCCLDQMVSEKLLPTLTRLRVLSLSHYKIARLPPDFFKNISHARFLDL-SRTELEKLPK 627

Query: 687 SICDLQSLQTLILDSCSALEDLPKDIRNLISLRYLWVTTNKLHLHKNGVGTMTSLRFL 713
           S+C + +LQTL+L  CS+L++LP DI NLI+LRYL +   KL       G + SL+ L
Sbjct: 628 SLCYMYNLQTLLLSYCSSLKELPTDISNLINLRYLDLIGTKLRQMPRRFGRLKSLQTL 677


HSP 2 Score: 74.3 bits (181), Expect = 4.0e-13
Identity = 73/293 (24.91%), Postives = 125/293 (42.66%), Query Frame = 1

Query: 3   SLKAILLDAEEKQEQNQRLHDWLKELQNVFYQVEDSIDEFKWEFFK--------QKDTGK 62
           ++ A+L+DAEEKQ  N  +  W+ EL++V Y  ED++D+   E  +          +  +
Sbjct: 48  TITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALRLNIGAESSSSNRLR 107

Query: 63  QVLAHFSCSSRISANKLKQNFKRNKVCDELNKIAANMYEFHLKVKHIGSISMETTHSFPS 122
           Q+    S    +  N      +  KV   L ++A+      LK         E T   P 
Sbjct: 108 QLRGRMSLGDFLDGNSEHLETRLEKVTIRLERLASQRNILGLK---------ELTAMIP- 167

Query: 123 ASEISTRHLKPSWQLLYPLKHASRNYDEAYDGILNVF---NKYTHGF--FHIVGEAGIGK 182
                 +   P+  L+   +   R+ D+  D I+      N   +G     IVG  G+GK
Sbjct: 168 ------KQRLPTTSLVDESEVFGRDDDK--DEIMRFLIPENGKDNGITVVAIVGIGGVGK 227

Query: 183 STMARFLYNHENVVDRYTRRYWVCVEEGFNTHRLMKEIYSHADNKETR-EDLTTEQLLSK 242
           +T+++ LYN ++V   +  + W  V E F+  ++ K++Y    ++     DL   Q+  K
Sbjct: 228 TTLSQLLYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVTSRPCEFTDLDVLQVKLK 287

Query: 243 FIRLQREETFLLVFQDLSITNLDKCSTSVLIKLLEMGHHDSKIIVTTQTEEIA 282
                    FLLV  DL   N +     +L +        S+I+VTT+++ +A
Sbjct: 288 ERLTGTGLPFLLVLDDL--WNENFADWDLLRQPFIHAAQGSQILVTTRSQRVA 320

BLAST of MELO3C009695 vs. TAIR10
Match: AT4G27220.1 (AT4G27220.1 NB-ARC domain-containing disease resistance protein)

HSP 1 Score: 123.2 bits (308), Expect = 7.6e-28
Identity = 90/326 (27.61%), Postives = 154/326 (47.24%), Query Frame = 1

Query: 409 NSPEVQNPELTK-IVDQLLEKCMGVPLAIKCLGSLLSSKTSIAEWK---NIEKKLMPQEK 468
           N  EV N +  K I   +  +C G+PLAI  +G  L  K  +  WK   N+ K+  P   
Sbjct: 284 NVGEVANSDNVKPIAKDVSHECCGLPLAIITIGRTLRGKPQVEVWKHTLNLLKRSAPSID 343

Query: 469 KEDGILHVLRVCYDQMPSQLKPCFLYCSQLPNDRIFSSNDMIQLWMANGLLHSPEEKNLA 528
            E+ I   L++ YD +   +K CFL+C+  P D     +++I  W+A GLL         
Sbjct: 344 TEEKIFGTLKLSYDFLQDNMKSCFLFCALFPEDYSIKVSELIMYWVAEGLLDGQHHYE-D 403

Query: 529 MENIGKKYFMELWSRCFIQEIEEHGLGYWIKLHPLIQKLAHKITQEQSEGS----WGNNH 588
           M N G      L   C +++ +       +K+H +++  A      Q EG          
Sbjct: 404 MMNEGVTLVERLKDSCLLEDGDSCDT---VKMHDVVRDFAIWFMSSQGEGFHSLVMAGRG 463

Query: 589 SKEITEIRSIAFQERNMVLPNA--SLTEKCIWRYKCLRLLYLGNADLQEIPNS-IGTLKY 648
             E  + + ++  +R  ++ N    L    I   + L LL  GN+ ++E+PN  +     
Sbjct: 464 LIEFPQDKFVSSVQRVSLMANKLERLPNNVIEGVETLVLLLQGNSHVKEVPNGFLQAFPN 523

Query: 649 LRYLDLHGNQKIKHLPDSICDLQSLQTLILDSCSALEDLPKDIRNLISLRYLWVTTNKLH 708
           LR LDL G  +I+ LPDS  +L SL++L+L +C  L +LP  + +L+ L++L +  + + 
Sbjct: 524 LRILDLSG-VRIRTLPDSFSNLHSLRSLVLRNCKKLRNLP-SLESLVKLQFLDLHESAIR 583

Query: 709 LHKNGVGTMTSLRFLAIGGCKNLENL 724
               G+  ++SLR++ +     L+++
Sbjct: 584 ELPRGLEALSSLRYICVSNTYQLQSI 603


HSP 2 Score: 48.9 bits (115), Expect = 1.8e-05
Identity = 44/138 (31.88%), Postives = 63/138 (45.65%), Query Frame = 1

Query: 577 GNNHSKEITEIRSIAFQERNMVLPNASLTEKCIWRYKCLRLLYLGNADLQEIPNSIGTLK 636
           GN+H KE+               PN  L       +  LR+L L    ++ +P+S   L 
Sbjct: 502 GNSHVKEV---------------PNGFLQA-----FPNLRILDLSGVRIRTLPDSFSNLH 561

Query: 637 YLRYLDLHGNQKIKHLPDSICDLQSLQTLILDSCSALEDLPKDIRNLISLRYLWVTTNKL 696
            LR L L   +K+++LP S+  L  LQ L L   SA+ +LP+ +  L SLRY+ V +N  
Sbjct: 562 SLRSLVLRNCKKLRNLP-SLESLVKLQFLDLHE-SAIRELPRGLEALSSLRYICV-SNTY 616

Query: 697 HLHKNGVGTMTSLRFLAI 715
            L     GT+  L  L +
Sbjct: 622 QLQSIPAGTILQLSSLEV 616

BLAST of MELO3C009695 vs. TAIR10
Match: AT3G14460.1 (AT3G14460.1 LRR and NB-ARC domains-containing disease resistance protein)

HSP 1 Score: 118.2 bits (295), Expect = 2.4e-26
Identity = 114/456 (25.00%), Postives = 204/456 (44.74%), Query Frame = 1

Query: 339 ETEKNLEKNPVAGDGESESSRLITKDAQNLDVSIPQVTECEPDRGYQTIFKLKKLSKQSS 398
           E++   E   VA     E S+++      +  ++ +          + I+++K ++ +  
Sbjct: 284 ESDSEWESFQVAFTDAEEGSKIVLTTRSEIVSTVAKA---------EKIYQMKLMTNEEC 343

Query: 399 SLLFKEYAFRN-SPEVQNPELTKIVDQLLEKCMGVPLAIKCLGSLLSSKTSIAEWKNIEK 458
             L   +AF N S    N EL  I  ++ E+C G+PLA + + S L SK +  +W  + K
Sbjct: 344 WELISRFAFGNISVGSINQELEGIGKRIAEQCKGLPLAARAIASHLRSKPNPDDWYAVSK 403

Query: 459 KLMPQEKKEDGILHVLRVCYDQMPSQLKPCFLYCSQLPNDRIFSSNDMIQLWMANGLLHS 518
                    + IL VL++ YD +P QLK CF  CS  P   +F   +++ LWMA  LL+ 
Sbjct: 404 NF---SSYTNSILPVLKLSYDSLPPQLKRCFALCSIFPKGHVFDREELVLLWMAIDLLYQ 463

Query: 519 PEEKNLAMENIGKKYFMELWSRCFIQEIEEHGLGYWIKLHPLIQKLAHKITQEQSEGSWG 578
           P      +E+IG  Y  +L ++ F Q ++     +   +H L+  LA  ++ +       
Sbjct: 464 PRSSR-RLEDIGNDYLGDLVAQSFFQRLDITMTSF--VMHDLMNDLAKAVSGDFCFRLED 523

Query: 579 NNHSKEITEIRSIAFQERNMVLPNASLTEKCIWRYKCLRLLYLGNADLQEIPNSIGTLKY 638
           +N  +  +  R  +F        +AS+  + I   + LR +   N+     P S+ +L+ 
Sbjct: 524 DNIPEIPSTTRHFSFSRSQC---DASVAFRSICGAEFLRTILPFNS-----PTSLESLQL 583

Query: 639 LRYLDLHGNQKIKHLPDSICDLQSLQTLILDSCSALEDLPKDIRNLISLRYLWVTTNKLH 698
                       K L   +  L  L+ L L S   + +LPK ++ L  LRYL +++ K+ 
Sbjct: 584 TE----------KVLNPLLNALSGLRILSL-SHYQITNLPKSLKGLKLLRYLDLSSTKIK 643

Query: 699 LHKNGVGTMTSLRFLAIGGCKNLENLFERPDCLAGLETLMIYNCNTLKLLP--NEMRYLK 758
                V T+ +L+ L +  C++L +L +    L  L  L +     +++ P   ++R L+
Sbjct: 644 ELPEFVCTLCNLQTLLLSNCRDLTSLPKSIAELINLRLLDLVGTPLVEMPPGIKKLRSLQ 703

Query: 759 SLQNLMIWRCKQLTLN-LEEVEFKLQRFTVKELPKV 791
            L N +I R     L+ L+E+        + EL  V
Sbjct: 704 KLSNFVIGRLSGAGLHELKELSHLRGTLRISELQNV 705


HSP 2 Score: 40.0 bits (92), Expect = 8.5e-03
Identity = 63/239 (26.36%), Postives = 109/239 (45.61%), Query Frame = 1

Query: 613 KCLRLLYLGNADLQEIPNSIGTLKYLRYLDLHGNQKIKHLPDSICDLQSLQTLILDSCSA 672
           K LR L L +  ++E+P  + TL  L+ L L   + +  LP SI +L +L+ L L   + 
Sbjct: 596 KLLRYLDLSSTKIKELPEFVCTLCNLQTLLLSNCRDLTSLPKSIAELINLRLLDLVG-TP 655

Query: 673 LEDLPKDIRNLISLRYLWVTTNKLHLHKNGVG-----TMTSLR-FLAIGGCKNLENLFER 732
           L ++P  I+ L SL+ L   +N +    +G G      ++ LR  L I   +N+    E 
Sbjct: 656 LVEMPPGIKKLRSLQKL---SNFVIGRLSGAGLHELKELSHLRGTLRISELQNVAFASEA 715

Query: 733 PDCLAGLETLMIYNCNTLKLLPNEMRYLKSLQNLMIWRCKQLTLNLEEVEFKLQRFTVKE 792
            D  AGL+     +   LK       ++    N +    K++ L + E    L+ F + E
Sbjct: 716 KD--AGLKRKPFLDGLILKWTVKGSGFVPGSFNALACDQKEV-LRMLEPHPHLKTFCI-E 775

Query: 793 LPKVESLPRWL-ENSIETLRTLQIINCPIRLMELPVDK----KYRALEIFLIHGSVRFD 841
             +  + P+WL ++S   + ++ + +C + +   PV +    KY ++E F I   V  D
Sbjct: 776 SYQGGAFPKWLGDSSFFGITSVTLSSCNLCISLPPVGQLPSLKYLSIEKFNILQKVGLD 826

BLAST of MELO3C009695 vs. TAIR10
Match: AT1G58390.1 (AT1G58390.1 Disease resistance protein (CC-NBS-LRR class) family)

HSP 1 Score: 108.2 bits (269), Expect = 2.5e-23
Identity = 102/372 (27.42%), Postives = 163/372 (43.82%), Query Frame = 1

Query: 388 FKLKKLSKQSSSLLFKEYAFRN---SPEVQNPELTKIVDQLLEKCMGVPLAIKCLGSLLS 447
           FK + L+ + S  LF+  AF     S    + E+  +  Q+L+ C G+PLAIK LG LL+
Sbjct: 316 FKPECLAIEDSWTLFQRIAFPKKDASESKVDEEMEDMGKQMLKHCGGLPLAIKVLGGLLA 375

Query: 448 SKTSIAEWKNIEKKLMPQ-----EKKEDGILHVLRVCYDQMPSQLKPCFLYCSQLPNDRI 507
           +K ++ +W+ +   +              I HVL + ++++PS LK CFLY +  P D  
Sbjct: 376 AKYTMHDWERLSVNIGSDIVGRTSSNNSSIYHVLSMSFEELPSYLKHCFLYLAHFPEDHK 435

Query: 508 FSSNDMIQLWMANGLLHSPEEKN-LAMENIGKKYFMEL-------WSR---------CFI 567
            +   +   W A G+  + +  N   ++++G+ Y  EL       W R         C +
Sbjct: 436 INVEKLSYCWAAEGISTAEDYHNGETIQDVGQSYLEELVRRNMIIWERDATASRFGTCHL 495

Query: 568 QEIEEHGLGYWIKLHPLIQKLAHKITQEQSEGSWGNNHS------------------KEI 627
            ++      +  K    +Q     +    S  S GN+ S                  ++I
Sbjct: 496 HDMMREVCLFKAKEENFLQIAVKSVGVTSS--STGNSQSPCRSRRLVYQCPTTLHVERDI 555

Query: 628 T--EIRSIAFQERNMVLPNASLTEKCIWRYKCLRLLYLGNADLQ--EIPNSIGTLKYLRY 687
              ++RS+     ++ + N  L      R K LR+L L   D +  ++P  IG L +LRY
Sbjct: 556 NNPKLRSLVVLWHDLWVENWKLLGTSFTRLKLLRVLDLFYVDFEGMKLPFGIGNLIHLRY 615

Query: 688 LDLHGNQKIKHLPDSICDLQSLQTLILDSCSALEDLPKDIRNLISLRYLWVTTNKLHLHK 713
           L L  + K+ HLP S+ +L  L  L LD  +    +P     +  LRYL +    LH+HK
Sbjct: 616 LSLQ-DAKVSHLPSSLGNLMLLIYLNLDVDTEFIFVPDVFMRMHELRYLKL---PLHMHK 675


HSP 2 Score: 67.4 bits (163), Expect = 4.9e-11
Identity = 70/308 (22.73%), Postives = 133/308 (43.18%), Query Frame = 1

Query: 1   MSSLKAILLDAEEKQEQNQRLHDWLKELQNVFYQVEDSIDEF--KWEFFKQKDTGKQVLA 60
           ++ L + L DA+ K+     + + ++E++ + Y  ED I+ +  K + +K      ++  
Sbjct: 38  LNLLSSFLKDADAKKHTTAVVRNVVEEIKEIVYDAEDIIETYLLKEKLWKTSGIKMRIRR 97

Query: 61  HFSCSSRISANKLKQNFKRNKVCDELNKIAANMYEFHLKVKHIGSISMETTHSFPSASEI 120
           H    S    N L     R ++ D    +  +M  F ++   +    M+     P     
Sbjct: 98  HACIISDRRRNALDVGGIRTRISD----VIRDMQSFGVQQAIVDGGYMQ-----PQGDR- 157

Query: 121 STRHLKPSWQLLYPLKHASRNYDEAYDGILNVFNKYTHGFF---------HIVGEAGIGK 180
             R ++ ++         S++Y+  + G L V  K   G+           I G  G+GK
Sbjct: 158 -QREMRQTF---------SKDYESDFVG-LEVNVKKLVGYLVDEENVQVVSITGMGGLGK 217

Query: 181 STMARFLYNHENVVDRYTRRYWVCVEEGFNTHRLMKEIYSHADNKETRED---LTTEQLL 240
           +T+AR ++NHE+V  ++ R  WVCV + F    + + I  +  ++E +++   +   +L 
Sbjct: 218 TTLARQVFNHEDVKHQFDRLAWVCVSQEFTRKNVWQMILQNLTSREKKDEILQMEEAELH 277

Query: 241 SKFIRLQREETFLLVFQDLSITNLDKCSTSVLIKLLEMGHHDSKIIVTTQTEEIA--KDI 293
            K  +L      L+VF D+      K     LIK +   +   K+++T+Q E +A   DI
Sbjct: 278 DKLFQLLETSKSLIVFDDIW-----KDEDWDLIKPIFPPNKGWKVLLTSQNESVAVRGDI 319


HSP 3 Score: 37.0 bits (84), Expect = 7.2e-02
Identity = 27/98 (27.55%), Postives = 47/98 (47.96%), Query Frame = 1

Query: 704 GTMTSLRFLAIGGCKNLENLFERPDCLAGLETLMIYNCNTLKLLPNEMRYLKSLQNLMI- 763
           G    L+ L I G    E        +  LETL I +C  LK +P+ +R++ SL+ +M+ 
Sbjct: 817 GGFPQLKKLEIVGLNKWEEWLVEEGSMPLLETLSILDCEELKEIPDGLRFIYSLELVMLG 876

Query: 764 --WRCKQLTLNLEEVEFKLQRFTVKELPKVESLPRWLE 799
             W+ K+ ++  E+       + V+ +P VE +  +L+
Sbjct: 877 TRWK-KKFSVGGEDY------YKVQHIPSVEFIGGYLK 907


HSP 4 Score: 36.6 bits (83), Expect = 9.4e-02
Identity = 39/134 (29.10%), Postives = 58/134 (43.28%), Query Frame = 1

Query: 615 LRLLYLGNADLQEIPNSIGTLKYLRYLDLHGNQKIKHLPDSICDLQSLQTLILDSCSALE 674
           LR L L +A +  +P+S+G L  L YL+L  + +   +PD    +  L+ L L      +
Sbjct: 611 LRYLSLQDAKVSHLPSSLGNLMLLIYLNLDVDTEFIFVPDVFMRMHELRYLKL-PLHMHK 670

Query: 675 DLPKDIRNLISLRYLWVTTNKLHLHKNGVGTMTSLRFLAIGGCK--NLENLFERPDCLAG 734
                +RNL+ L  L V  +  H     +  MT L  LAI   +  + E L      L  
Sbjct: 671 KTRLSLRNLVKLETL-VYFSTWHSSSKDLCGMTRLMTLAIRLTRVTSTETLSASISGLRN 730

Query: 735 LETLMIYNCNTLKL 747
           LE L I   ++ K+
Sbjct: 731 LEYLYIVGTHSKKM 742

BLAST of MELO3C009695 vs. TAIR10
Match: AT1G59620.1 (AT1G59620.1 Disease resistance protein (CC-NBS-LRR class) family)

HSP 1 Score: 105.1 bits (261), Expect = 2.1e-22
Identity = 122/498 (24.50%), Postives = 215/498 (43.17%), Query Frame = 1

Query: 387 IFKLKKLSKQSSSLLFKEYAF--RNSPEVQNPE-LTKIVDQLLEKCMGVPLAIKCLGSLL 446
           IFK   L+ + S  +F+   F   N+ E +  E + ++  Q+++ C G+PLA+K LG LL
Sbjct: 279 IFKPDCLTPEESWTIFRRIVFPGENTTEYKVDEKMEELGKQMIKHCGGLPLALKVLGGLL 338

Query: 447 SSKTSIAEWK----NIEKKLMP----QEKKEDGILHVLRVCYDQMPSQLKPCFLYCSQLP 506
               ++ EWK    NI+  ++      +K    + H+L + ++++P  LK CFLY +Q P
Sbjct: 339 VVHFTLDEWKRIYGNIKSHIVGGTSFNDKNMSSVYHILHLSFEELPIYLKHCFLYLAQFP 398

Query: 507 NDRIFSSNDMIQLWMANGLLHSPEEKNLAMENIGKKYFMELWSR-CFIQEIEEHGLGY-W 566
            D       +   W A G+          +  +G  Y  EL  R   I E +     +  
Sbjct: 399 EDFTIDLEKLSYYWAAEGMPRPRYYDGATIRKVGDGYIEELVKRNMVISERDARTRRFET 458

Query: 567 IKLHPLIQKLAHKITQEQSEGSWGNNHSKEITEIRSIAFQE----------RNMVLPNAS 626
             LH +++++   + + + E      +SK  ++ R +  +           +N  L +  
Sbjct: 459 CHLHDIVREVC--LLKAEEENLIETENSKSPSKPRRLVVKGGDKTDMEGKLKNPKLRSLL 518

Query: 627 LTEKC-------IW--RYKCLRLLYLGNADL-QEIPNSIGTLKYLRYLDLHGNQKIKHLP 686
             E+        +W  R + +R+L L   +   E+P+SIG L +LRYL L+   K  HLP
Sbjct: 519 FIEELGGYRGFEVWFTRLQLMRVLDLHGVEFGGELPSSIGLLIHLRYLSLY-RAKASHLP 578

Query: 687 DSICDLQSLQTLILDSC---SALEDLPKDIRNLISLRYLWVTTNKLHLHKNGVGTMTSLR 746
            S+ +L+ L  L L+ C   S    +P  ++ ++ L+YL   +  L +    +G    L+
Sbjct: 579 SSMQNLKML--LYLNLCVQESCYIYIPNFLKEMLELKYL---SLPLRMDDKSMGEWGDLQ 638

Query: 747 FLAIGGCKNLENLFERPDCLAGLETLMIYNCNTL--KLLPNEMRYLKSLQNLMIW----- 806
           F                  +  L  L IY    L  K L + +  L+ L+NL I      
Sbjct: 639 F------------------MTRLRALSIYIRGRLNMKTLSSSLSKLRDLENLTICYYPMY 698

Query: 807 ----RCKQLTLNLEEVEFKLQRFTVKELPKVESLPRWLENSIETLRTLQIINCPIRLMEL 837
                 + L L+ ++++    R  +  LP  +  P W       LR + +  C ++   +
Sbjct: 699 APMSGIEGLVLDCDQLKHLNLRIYMPRLPDEQHFP-W------HLRNISLAECCLKEDPM 743


HSP 2 Score: 55.1 bits (131), Expect = 2.5e-07
Identity = 27/85 (31.76%), Postives = 42/85 (49.41%), Query Frame = 1

Query: 161 IVGEAGIGKSTMARFLYNHENVVDRYTRRYWVCVEEGFNTHRLMKEIYSHADNKETREDL 220
           I G  GIGK+T+AR ++NHE V   + +  WVCV + F    + + I      +  + ++
Sbjct: 155 ITGMGGIGKTTLARQVFNHETVKSHFAQLAWVCVSQQFTRKYVWQTILRKVGPEYIKLEM 214

Query: 221 TTEQLLSKFIRLQREETFLLVFQDL 246
           T ++L  K  RL      L+V  D+
Sbjct: 215 TEDELQEKLFRLLGTRKALIVLDDI 239

BLAST of MELO3C009695 vs. NCBI nr
Match: gi|659084955|ref|XP_008443163.1| (PREDICTED: probable disease resistance protein At1g59620 isoform X1 [Cucumis melo])

HSP 1 Score: 1751.5 bits (4535), Expect = 0.0e+00
Identity = 864/864 (100.00%), Postives = 864/864 (100.00%), Query Frame = 1

Query: 1   MSSLKAILLDAEEKQEQNQRLHDWLKELQNVFYQVEDSIDEFKWEFFKQKDTGKQVLAHF 60
           MSSLKAILLDAEEKQEQNQRLHDWLKELQNVFYQVEDSIDEFKWEFFKQKDTGKQVLAHF
Sbjct: 30  MSSLKAILLDAEEKQEQNQRLHDWLKELQNVFYQVEDSIDEFKWEFFKQKDTGKQVLAHF 89

Query: 61  SCSSRISANKLKQNFKRNKVCDELNKIAANMYEFHLKVKHIGSISMETTHSFPSASEIST 120
           SCSSRISANKLKQNFKRNKVCDELNKIAANMYEFHLKVKHIGSISMETTHSFPSASEIST
Sbjct: 90  SCSSRISANKLKQNFKRNKVCDELNKIAANMYEFHLKVKHIGSISMETTHSFPSASEIST 149

Query: 121 RHLKPSWQLLYPLKHASRNYDEAYDGILNVFNKYTHGFFHIVGEAGIGKSTMARFLYNHE 180
           RHLKPSWQLLYPLKHASRNYDEAYDGILNVFNKYTHGFFHIVGEAGIGKSTMARFLYNHE
Sbjct: 150 RHLKPSWQLLYPLKHASRNYDEAYDGILNVFNKYTHGFFHIVGEAGIGKSTMARFLYNHE 209

Query: 181 NVVDRYTRRYWVCVEEGFNTHRLMKEIYSHADNKETREDLTTEQLLSKFIRLQREETFLL 240
           NVVDRYTRRYWVCVEEGFNTHRLMKEIYSHADNKETREDLTTEQLLSKFIRLQREETFLL
Sbjct: 210 NVVDRYTRRYWVCVEEGFNTHRLMKEIYSHADNKETREDLTTEQLLSKFIRLQREETFLL 269

Query: 241 VFQDLSITNLDKCSTSVLIKLLEMGHHDSKIIVTTQTEEIAKDIDVHGYKTEIQSKKNLA 300
           VFQDLSITNLDKCSTSVLIKLLEMGHHDSKIIVTTQTEEIAKDIDVHGYKTEIQSKKNLA
Sbjct: 270 VFQDLSITNLDKCSTSVLIKLLEMGHHDSKIIVTTQTEEIAKDIDVHGYKTEIQSKKNLA 329

Query: 301 NGDEVSADKQSLTKNTQNQAVPGSQTEETAGAIANMIYETEKNLEKNPVAGDGESESSRL 360
           NGDEVSADKQSLTKNTQNQAVPGSQTEETAGAIANMIYETEKNLEKNPVAGDGESESSRL
Sbjct: 330 NGDEVSADKQSLTKNTQNQAVPGSQTEETAGAIANMIYETEKNLEKNPVAGDGESESSRL 389

Query: 361 ITKDAQNLDVSIPQVTECEPDRGYQTIFKLKKLSKQSSSLLFKEYAFRNSPEVQNPELTK 420
           ITKDAQNLDVSIPQVTECEPDRGYQTIFKLKKLSKQSSSLLFKEYAFRNSPEVQNPELTK
Sbjct: 390 ITKDAQNLDVSIPQVTECEPDRGYQTIFKLKKLSKQSSSLLFKEYAFRNSPEVQNPELTK 449

Query: 421 IVDQLLEKCMGVPLAIKCLGSLLSSKTSIAEWKNIEKKLMPQEKKEDGILHVLRVCYDQM 480
           IVDQLLEKCMGVPLAIKCLGSLLSSKTSIAEWKNIEKKLMPQEKKEDGILHVLRVCYDQM
Sbjct: 450 IVDQLLEKCMGVPLAIKCLGSLLSSKTSIAEWKNIEKKLMPQEKKEDGILHVLRVCYDQM 509

Query: 481 PSQLKPCFLYCSQLPNDRIFSSNDMIQLWMANGLLHSPEEKNLAMENIGKKYFMELWSRC 540
           PSQLKPCFLYCSQLPNDRIFSSNDMIQLWMANGLLHSPEEKNLAMENIGKKYFMELWSRC
Sbjct: 510 PSQLKPCFLYCSQLPNDRIFSSNDMIQLWMANGLLHSPEEKNLAMENIGKKYFMELWSRC 569

Query: 541 FIQEIEEHGLGYWIKLHPLIQKLAHKITQEQSEGSWGNNHSKEITEIRSIAFQERNMVLP 600
           FIQEIEEHGLGYWIKLHPLIQKLAHKITQEQSEGSWGNNHSKEITEIRSIAFQERNMVLP
Sbjct: 570 FIQEIEEHGLGYWIKLHPLIQKLAHKITQEQSEGSWGNNHSKEITEIRSIAFQERNMVLP 629

Query: 601 NASLTEKCIWRYKCLRLLYLGNADLQEIPNSIGTLKYLRYLDLHGNQKIKHLPDSICDLQ 660
           NASLTEKCIWRYKCLRLLYLGNADLQEIPNSIGTLKYLRYLDLHGNQKIKHLPDSICDLQ
Sbjct: 630 NASLTEKCIWRYKCLRLLYLGNADLQEIPNSIGTLKYLRYLDLHGNQKIKHLPDSICDLQ 689

Query: 661 SLQTLILDSCSALEDLPKDIRNLISLRYLWVTTNKLHLHKNGVGTMTSLRFLAIGGCKNL 720
           SLQTLILDSCSALEDLPKDIRNLISLRYLWVTTNKLHLHKNGVGTMTSLRFLAIGGCKNL
Sbjct: 690 SLQTLILDSCSALEDLPKDIRNLISLRYLWVTTNKLHLHKNGVGTMTSLRFLAIGGCKNL 749

Query: 721 ENLFERPDCLAGLETLMIYNCNTLKLLPNEMRYLKSLQNLMIWRCKQLTLNLEEVEFKLQ 780
           ENLFERPDCLAGLETLMIYNCNTLKLLPNEMRYLKSLQNLMIWRCKQLTLNLEEVEFKLQ
Sbjct: 750 ENLFERPDCLAGLETLMIYNCNTLKLLPNEMRYLKSLQNLMIWRCKQLTLNLEEVEFKLQ 809

Query: 781 RFTVKELPKVESLPRWLENSIETLRTLQIINCPIRLMELPVDKKYRALEIFLIHGSVRFD 840
           RFTVKELPKVESLPRWLENSIETLRTLQIINCPIRLMELPVDKKYRALEIFLIHGSVRFD
Sbjct: 810 RFTVKELPKVESLPRWLENSIETLRTLQIINCPIRLMELPVDKKYRALEIFLIHGSVRFD 869

Query: 841 TMPDYDFEHRNLTIFRGNEMNIHY 865
           TMPDYDFEHRNLTIFRGNEMNIHY
Sbjct: 870 TMPDYDFEHRNLTIFRGNEMNIHY 893

BLAST of MELO3C009695 vs. NCBI nr
Match: gi|659084957|ref|XP_008443164.1| (PREDICTED: probable disease resistance protein At1g59620 isoform X2 [Cucumis melo])

HSP 1 Score: 1670.6 bits (4325), Expect = 0.0e+00
Identity = 827/837 (98.81%), Postives = 831/837 (99.28%), Query Frame = 1

Query: 1   MSSLKAILLDAEEKQEQNQRLHDWLKELQNVFYQVEDSIDEFKWEFFKQKDTGKQVLAHF 60
           MSSLKAILLDAEEKQEQNQRLHDWLKELQNVFYQVEDSIDEFKWEFFKQKDTGKQVLAHF
Sbjct: 30  MSSLKAILLDAEEKQEQNQRLHDWLKELQNVFYQVEDSIDEFKWEFFKQKDTGKQVLAHF 89

Query: 61  SCSSRISANKLKQNFKRNKVCDELNKIAANMYEFHLKVKHIGSISMETTHSFPSASEIST 120
           SCSSRISANKLKQNFKRNKVCDELNKIAANMYEFHLKVKHIGSISMETTHSFPSASEIST
Sbjct: 90  SCSSRISANKLKQNFKRNKVCDELNKIAANMYEFHLKVKHIGSISMETTHSFPSASEIST 149

Query: 121 RHLKPSWQLLYPLKHASRNYDEAYDGILNVFNKYTHGFFHIVGEAGIGKSTMARFLYNHE 180
           RHLKPSWQLLYPLKHASRNYDEAYDGILNVFNKYTHGFFHIVGEAGIGKSTMARFLYNHE
Sbjct: 150 RHLKPSWQLLYPLKHASRNYDEAYDGILNVFNKYTHGFFHIVGEAGIGKSTMARFLYNHE 209

Query: 181 NVVDRYTRRYWVCVEEGFNTHRLMKEIYSHADNKETREDLTTEQLLSKFIRLQREETFLL 240
           NVVDRYTRRYWVCVEEGFNTHRLMKEIYSHADNKETREDLTTEQLLSKFIRLQREETFLL
Sbjct: 210 NVVDRYTRRYWVCVEEGFNTHRLMKEIYSHADNKETREDLTTEQLLSKFIRLQREETFLL 269

Query: 241 VFQDLSITNLDKCSTSVLIKLLEMGHHDSKIIVTTQTEEIAKDIDVHGYKTEIQSKKNLA 300
           VFQDLSITNLDKCSTSVLIKLLEMGHHDSKIIVTTQTEEIAKDIDVHGYKTEIQSKKNLA
Sbjct: 270 VFQDLSITNLDKCSTSVLIKLLEMGHHDSKIIVTTQTEEIAKDIDVHGYKTEIQSKKNLA 329

Query: 301 NGDEVSADKQSLTKNTQNQAVPGSQTEETAGAIANMIYETEKNLEKNPVAGDGESESSRL 360
           NGDEVSADKQSLTKNTQNQAVPGSQTEETAGAIANMIYETEKNLEKNPVAGDGESESSRL
Sbjct: 330 NGDEVSADKQSLTKNTQNQAVPGSQTEETAGAIANMIYETEKNLEKNPVAGDGESESSRL 389

Query: 361 ITKDAQNLDVSIPQVTECEPDRGYQTIFKLKKLSKQSSSLLFKEYAFRNSPEVQNPELTK 420
           ITKDAQNLDVSIPQVTECEPDRGYQTIFKLKKLSKQSSSLLFKEYAFRNSPEVQNPELTK
Sbjct: 390 ITKDAQNLDVSIPQVTECEPDRGYQTIFKLKKLSKQSSSLLFKEYAFRNSPEVQNPELTK 449

Query: 421 IVDQLLEKCMGVPLAIKCLGSLLSSKTSIAEWKNIEKKLMPQEKKEDGILHVLRVCYDQM 480
           IVDQLLEKCMGVPLAIKCLGSLLSSKTSIAEWKNIEKKLMPQEKKEDGILHVLRVCYDQM
Sbjct: 450 IVDQLLEKCMGVPLAIKCLGSLLSSKTSIAEWKNIEKKLMPQEKKEDGILHVLRVCYDQM 509

Query: 481 PSQLKPCFLYCSQLPNDRIFSSNDMIQLWMANGLLHSPEEKNLAMENIGKKYFMELWSRC 540
           PSQLKPCFLYCSQLPNDRIFSSNDMIQLWMANGLLHSPEEKNLAMENIGKKYFMELWSRC
Sbjct: 510 PSQLKPCFLYCSQLPNDRIFSSNDMIQLWMANGLLHSPEEKNLAMENIGKKYFMELWSRC 569

Query: 541 FIQEIEEHGLGYWIKLHPLIQKLAHKITQEQSEGSWGNNHSKEITEIRSIAFQERNMVLP 600
           FIQEIEEHGLGYWIKLHPLIQKLAHKITQEQSEGSWGNNHSKEITEIRSIAFQERNMVLP
Sbjct: 570 FIQEIEEHGLGYWIKLHPLIQKLAHKITQEQSEGSWGNNHSKEITEIRSIAFQERNMVLP 629

Query: 601 NASLTEKCIWRYKCLRLLYLGNADLQEIPNSIGTLKYLRYLDLHGNQKIKHLPDSICDLQ 660
           NASLTEKCIWRYKCLRLLYLGNADLQEIPNSIGTLKYLRYLDLHGNQKIKHLPDSICDLQ
Sbjct: 630 NASLTEKCIWRYKCLRLLYLGNADLQEIPNSIGTLKYLRYLDLHGNQKIKHLPDSICDLQ 689

Query: 661 SLQTLILDSCSALEDLPKDIRNLISLRYLWVTTNKLHLHKNGVGTMTSLRFLAIGGCKNL 720
           SLQTLILDSCSALEDLPKDIRNLISLRYLWVTTNKLHLHKNGVGTMTSLRFLAIGGCKNL
Sbjct: 690 SLQTLILDSCSALEDLPKDIRNLISLRYLWVTTNKLHLHKNGVGTMTSLRFLAIGGCKNL 749

Query: 721 ENLFERPDCLAGLETLMIYNCNTLKLLPNEMRYLKSLQNLMIWRCKQLTLNLEEVEFKLQ 780
           ENLFERPDCLAGLETLMIYNCNTLKLLPNEMRYLKSLQNLMIWRCKQLTLNLEEVEFKLQ
Sbjct: 750 ENLFERPDCLAGLETLMIYNCNTLKLLPNEMRYLKSLQNLMIWRCKQLTLNLEEVEFKLQ 809

Query: 781 RFTVKELPKVESLPRWLENSIETLRTLQIINCPIRLMELPVDKKYRALEIFLIHGSV 838
           RFTVKELPKVESLPRWLENSIETLRTLQIINCPIRLMELP  K Y+A+E  +I G+V
Sbjct: 810 RFTVKELPKVESLPRWLENSIETLRTLQIINCPIRLMELPGIKMYKAVESKIIQGAV 866

BLAST of MELO3C009695 vs. NCBI nr
Match: gi|700204242|gb|KGN59375.1| (hypothetical protein Csa_3G815400 [Cucumis sativus])

HSP 1 Score: 1371.3 bits (3548), Expect = 0.0e+00
Identity = 704/875 (80.46%), Postives = 749/875 (85.60%), Query Frame = 1

Query: 1   MSSLKAILLDAEEKQEQNQRLHDWLKELQNVFYQVEDSIDEFKWEFFKQKDTGKQVLAHF 60
           MSSLKA+LLDAEEKQEQNQRL +WLKELQNVFYQVEDSIDEFKWE FKQKD GKQVLA F
Sbjct: 30  MSSLKAVLLDAEEKQEQNQRLQNWLKELQNVFYQVEDSIDEFKWEIFKQKDIGKQVLAPF 89

Query: 61  SCSSRISANKLKQNFKRNKVCDELNKIAANMYEFHLKVKHIGSISMETTHSFPSASEIST 120
           SCS++ISANKLKQ  KR +VCDELN IA  MYEFHLKVKHI SISMETTH+FPSASEIS 
Sbjct: 90  SCSNQISANKLKQKCKRKQVCDELNNIATRMYEFHLKVKHIDSISMETTHTFPSASEISI 149

Query: 121 RHLKPSWQLLYPLKHASRNYDEAYDGILNVFNKYTHGFFHIVGEAGIGKSTMARFLYNHE 180
           RHLKPSWQLLYPL  ASR YDE YDGILNVFN+ TH  FHIVGEAGIGKST+ARFLYNH 
Sbjct: 150 RHLKPSWQLLYPLIDASRTYDEIYDGILNVFNECTH-VFHIVGEAGIGKSTVARFLYNHN 209

Query: 181 NVVDRYTRRYWVCVEEGFNTHRLMKEIYSHADNKETREDLTTEQLLSKFIRLQREETFLL 240
           NVV +YT RYWVCVEEGFN HRL+KE+YSHADNKE  EDLTTEQLLSK  RL + ETFLL
Sbjct: 210 NVVGKYTSRYWVCVEEGFNKHRLVKEVYSHADNKEICEDLTTEQLLSKVKRLLKVETFLL 269

Query: 241 VFQDLSITNLDKCSTSVLIKLLEMGHHDSKIIVTTQTEEIAKDIDVHGYKTEIQSKKNLA 300
           VFQDLSITNL   S  VL +LLEMG H SKIIVTTQTEEIA  +   GYKTE +SK+NLA
Sbjct: 270 VFQDLSITNLKDRSPLVLNELLEMGQHGSKIIVTTQTEEIANYLQDRGYKTERRSKENLA 329

Query: 301 NGDEVSADKQSLTKNTQNQAVPGSQTEETAGAIANMIYETEKNLEKNPVAGDGESESSRL 360
           NGD VSA  QSLTKNTQNQAVPGSQ EETA AIAN IYE   +L                
Sbjct: 330 NGDRVSAHNQSLTKNTQNQAVPGSQIEETADAIANKIYEHSLSL---------------- 389

Query: 361 ITKDAQNLDVSIPQVTEC---------------EPDRGYQTIFKLKKLSKQSSSLLFKEY 420
            T+DAQNL+V IPQVTE                + D  YQTIFKL+KLSKQSSS LFKEY
Sbjct: 390 -TEDAQNLEVPIPQVTEDTNIGLGINPDIPPIKQDDTEYQTIFKLEKLSKQSSSRLFKEY 449

Query: 421 AFRNSPEVQNPELTKIVDQLLEKCMGVPLAIKCLGSLLSSKTSIAEWKNIEKKLMPQEKK 480
           AFRN  EV+NPELTKIVDQLLEKCMGVPLAIKCLGSLLSS+TSIA+WKNIE+KL  QEKK
Sbjct: 450 AFRNRQEVENPELTKIVDQLLEKCMGVPLAIKCLGSLLSSETSIAKWKNIEEKLSLQEKK 509

Query: 481 EDGILHVLRVCYDQMPSQLKPCFLYCSQLPNDRIFSSNDMIQLWMANGLLHSPEEKNLAM 540
           E+GILHVLRVCYDQMPSQLKPCFL+CSQLPNDRI SSNDMIQLWMANGLLHSPEEKN  M
Sbjct: 510 ENGILHVLRVCYDQMPSQLKPCFLHCSQLPNDRIISSNDMIQLWMANGLLHSPEEKNSTM 569

Query: 541 ENIGKKYFMELWSRCFIQEIEEHGLGYWIKLHPLIQKLAHKITQEQSEGSWGNNHSKEIT 600
           ENIG+KYFMELWSR FIQEIEEHGLGYWIKLHPLIQKLAHKITQEQSEGS GNNH KE+T
Sbjct: 570 ENIGEKYFMELWSRYFIQEIEEHGLGYWIKLHPLIQKLAHKITQEQSEGSGGNNHPKEVT 629

Query: 601 EIRSIAFQERNMVLPNASLTEKCIWRYKCLRLLYLGNADLQEIPNSIGTLKYLRYLDLHG 660
           EIRSIAFQERNMVLPNASLTEKCIW+YK LRLLYL NADLQEIPNSIGTLKYLRYLDLHG
Sbjct: 630 EIRSIAFQERNMVLPNASLTEKCIWKYKGLRLLYLSNADLQEIPNSIGTLKYLRYLDLHG 689

Query: 661 NQKIKHLPDSICDLQSLQTLILDSCSALEDLPKDIRNLISLRYLWVTTNKLHLHKNGVGT 720
           N KIKHLP+SIC+LQSLQTLIL SCSALEDLPKDIRNLISLRYLWVTTNKL L KNGVGT
Sbjct: 690 NTKIKHLPNSICNLQSLQTLILGSCSALEDLPKDIRNLISLRYLWVTTNKLRLDKNGVGT 749

Query: 721 MTSLRFLAIGGCKNLENLFERPDCLAGLETLMIYNCNTLKLLPNEMRYLKSLQNLMIWRC 780
           M SLRFLAIGGC NLENLFERPDCLAGLETLMIYNC TLKLLP+EMRYLKSLQNLMIW C
Sbjct: 750 MNSLRFLAIGGCNNLENLFERPDCLAGLETLMIYNCTTLKLLPDEMRYLKSLQNLMIWSC 809

Query: 781 KQLTLNLEEVEFKLQRFTVKELPKVESLPRWLENSIETLRTLQIINCPIRLMELPVDKKY 840
           KQLTLNL+EVEFKLQRFT+KELP+VE LP+WLENS ETLRTLQIINCPIR+ME    +KY
Sbjct: 810 KQLTLNLKEVEFKLQRFTIKELPRVERLPQWLENSAETLRTLQIINCPIRIMERQGIEKY 869

Query: 841 RALEIFLIHGSVRFD-TMPDYDFEHRNLTIFRGNE 860
            A+E  +I+G+VRF+   P YDFEHRNL +  GNE
Sbjct: 870 EAVENTIIYGAVRFEMAPPGYDFEHRNLAVRNGNE 886

BLAST of MELO3C009695 vs. NCBI nr
Match: gi|778685015|ref|XP_004136758.2| (PREDICTED: putative disease resistance protein RGA1 [Cucumis sativus])

HSP 1 Score: 1371.3 bits (3548), Expect = 0.0e+00
Identity = 704/875 (80.46%), Postives = 749/875 (85.60%), Query Frame = 1

Query: 1   MSSLKAILLDAEEKQEQNQRLHDWLKELQNVFYQVEDSIDEFKWEFFKQKDTGKQVLAHF 60
           MSSLKA+LLDAEEKQEQNQRL +WLKELQNVFYQVEDSIDEFKWE FKQKD GKQVLA F
Sbjct: 1   MSSLKAVLLDAEEKQEQNQRLQNWLKELQNVFYQVEDSIDEFKWEIFKQKDIGKQVLAPF 60

Query: 61  SCSSRISANKLKQNFKRNKVCDELNKIAANMYEFHLKVKHIGSISMETTHSFPSASEIST 120
           SCS++ISANKLKQ  KR +VCDELN IA  MYEFHLKVKHI SISMETTH+FPSASEIS 
Sbjct: 61  SCSNQISANKLKQKCKRKQVCDELNNIATRMYEFHLKVKHIDSISMETTHTFPSASEISI 120

Query: 121 RHLKPSWQLLYPLKHASRNYDEAYDGILNVFNKYTHGFFHIVGEAGIGKSTMARFLYNHE 180
           RHLKPSWQLLYPL  ASR YDE YDGILNVFN+ TH  FHIVGEAGIGKST+ARFLYNH 
Sbjct: 121 RHLKPSWQLLYPLIDASRTYDEIYDGILNVFNECTH-VFHIVGEAGIGKSTVARFLYNHN 180

Query: 181 NVVDRYTRRYWVCVEEGFNTHRLMKEIYSHADNKETREDLTTEQLLSKFIRLQREETFLL 240
           NVV +YT RYWVCVEEGFN HRL+KE+YSHADNKE  EDLTTEQLLSK  RL + ETFLL
Sbjct: 181 NVVGKYTSRYWVCVEEGFNKHRLVKEVYSHADNKEICEDLTTEQLLSKVKRLLKVETFLL 240

Query: 241 VFQDLSITNLDKCSTSVLIKLLEMGHHDSKIIVTTQTEEIAKDIDVHGYKTEIQSKKNLA 300
           VFQDLSITNL   S  VL +LLEMG H SKIIVTTQTEEIA  +   GYKTE +SK+NLA
Sbjct: 241 VFQDLSITNLKDRSPLVLNELLEMGQHGSKIIVTTQTEEIANYLQDRGYKTERRSKENLA 300

Query: 301 NGDEVSADKQSLTKNTQNQAVPGSQTEETAGAIANMIYETEKNLEKNPVAGDGESESSRL 360
           NGD VSA  QSLTKNTQNQAVPGSQ EETA AIAN IYE   +L                
Sbjct: 301 NGDRVSAHNQSLTKNTQNQAVPGSQIEETADAIANKIYEHSLSL---------------- 360

Query: 361 ITKDAQNLDVSIPQVTEC---------------EPDRGYQTIFKLKKLSKQSSSLLFKEY 420
            T+DAQNL+V IPQVTE                + D  YQTIFKL+KLSKQSSS LFKEY
Sbjct: 361 -TEDAQNLEVPIPQVTEDTNIGLGINPDIPPIKQDDTEYQTIFKLEKLSKQSSSRLFKEY 420

Query: 421 AFRNSPEVQNPELTKIVDQLLEKCMGVPLAIKCLGSLLSSKTSIAEWKNIEKKLMPQEKK 480
           AFRN  EV+NPELTKIVDQLLEKCMGVPLAIKCLGSLLSS+TSIA+WKNIE+KL  QEKK
Sbjct: 421 AFRNRQEVENPELTKIVDQLLEKCMGVPLAIKCLGSLLSSETSIAKWKNIEEKLSLQEKK 480

Query: 481 EDGILHVLRVCYDQMPSQLKPCFLYCSQLPNDRIFSSNDMIQLWMANGLLHSPEEKNLAM 540
           E+GILHVLRVCYDQMPSQLKPCFL+CSQLPNDRI SSNDMIQLWMANGLLHSPEEKN  M
Sbjct: 481 ENGILHVLRVCYDQMPSQLKPCFLHCSQLPNDRIISSNDMIQLWMANGLLHSPEEKNSTM 540

Query: 541 ENIGKKYFMELWSRCFIQEIEEHGLGYWIKLHPLIQKLAHKITQEQSEGSWGNNHSKEIT 600
           ENIG+KYFMELWSR FIQEIEEHGLGYWIKLHPLIQKLAHKITQEQSEGS GNNH KE+T
Sbjct: 541 ENIGEKYFMELWSRYFIQEIEEHGLGYWIKLHPLIQKLAHKITQEQSEGSGGNNHPKEVT 600

Query: 601 EIRSIAFQERNMVLPNASLTEKCIWRYKCLRLLYLGNADLQEIPNSIGTLKYLRYLDLHG 660
           EIRSIAFQERNMVLPNASLTEKCIW+YK LRLLYL NADLQEIPNSIGTLKYLRYLDLHG
Sbjct: 601 EIRSIAFQERNMVLPNASLTEKCIWKYKGLRLLYLSNADLQEIPNSIGTLKYLRYLDLHG 660

Query: 661 NQKIKHLPDSICDLQSLQTLILDSCSALEDLPKDIRNLISLRYLWVTTNKLHLHKNGVGT 720
           N KIKHLP+SIC+LQSLQTLIL SCSALEDLPKDIRNLISLRYLWVTTNKL L KNGVGT
Sbjct: 661 NTKIKHLPNSICNLQSLQTLILGSCSALEDLPKDIRNLISLRYLWVTTNKLRLDKNGVGT 720

Query: 721 MTSLRFLAIGGCKNLENLFERPDCLAGLETLMIYNCNTLKLLPNEMRYLKSLQNLMIWRC 780
           M SLRFLAIGGC NLENLFERPDCLAGLETLMIYNC TLKLLP+EMRYLKSLQNLMIW C
Sbjct: 721 MNSLRFLAIGGCNNLENLFERPDCLAGLETLMIYNCTTLKLLPDEMRYLKSLQNLMIWSC 780

Query: 781 KQLTLNLEEVEFKLQRFTVKELPKVESLPRWLENSIETLRTLQIINCPIRLMELPVDKKY 840
           KQLTLNL+EVEFKLQRFT+KELP+VE LP+WLENS ETLRTLQIINCPIR+ME    +KY
Sbjct: 781 KQLTLNLKEVEFKLQRFTIKELPRVERLPQWLENSAETLRTLQIINCPIRIMERQGIEKY 840

Query: 841 RALEIFLIHGSVRFD-TMPDYDFEHRNLTIFRGNE 860
            A+E  +I+G+VRF+   P YDFEHRNL +  GNE
Sbjct: 841 EAVENTIIYGAVRFEMAPPGYDFEHRNLAVRNGNE 857

BLAST of MELO3C009695 vs. NCBI nr
Match: gi|700204239|gb|KGN59372.1| (hypothetical protein Csa_3G814390 [Cucumis sativus])

HSP 1 Score: 679.5 bits (1752), Expect = 7.8e-192
Identity = 408/859 (47.50%), Postives = 540/859 (62.86%), Query Frame = 1

Query: 1   MSSLKAILLDAEEKQEQNQRLHDWLKELQNVFYQVEDSIDEFKWEFFKQKDTGKQV-LAH 60
           M SLK IL DAE K+E    L+DWL++LQ+VF Q+E+ + E   E  KQ+ TGK V L  
Sbjct: 30  MLSLKTILKDAE-KEEYRHCLNDWLQKLQSVFLQIEELLYESNREVKKQEATGKWVFLPS 89

Query: 61  FSCSSRISANKLKQNFKRNKVCDELNKIAANMYEFHLKVKHIGSISMETTHSFPSASEIS 120
           F+ S      ++ Q  K  K+CD+L++IA++MY F+L        +METTHSF SA+E+S
Sbjct: 90  FNFS------QIDQTKKMMKLCDDLDEIASHMYGFNLT-------NMETTHSFLSATEVS 149

Query: 121 TRHLKPSWQLLYPLKHASRNY-DEAYDGILNVFNKYTHGFFHIVGEAGIGKSTMARFLYN 180
           TR +KPSWQLLY L +A + + D+ Y   L+ F K THG FHIVGE GIGK+T+A+F YN
Sbjct: 150 TRLMKPSWQLLYSLTNAPKVFQDKRYHNFLDHFKKSTHGLFHIVGEPGIGKTTLAKFFYN 209

Query: 181 HENVVDRYTRRYWVCVEEGFNTHRLMKEIYSHADNKETREDLTTEQLLSKFIRLQREETF 240
             N+V+ +  R W+CV+E F+                                 QR    
Sbjct: 210 --NLVNTFPSRLWICVKEEFDP--------------------------------QRLIKE 269

Query: 241 LLVFQDLSITNLDKCSTSVLIKLLEMGHHDSKIIVTTQTEEIAKDIDVHGYKTEIQSKKN 300
           +L F    +T  D  +   L   ++    D K ++  Q      DI +          KN
Sbjct: 270 MLSFSHCQVT-CDNLTEKQLCFAVQQFLRDKKFLIVFQ------DISI----------KN 329

Query: 301 LANGDEVSADKQSLTK-NTQNQAVPGSQTEETAGAIA-NMIYETEKNLEKNPVAGDGESE 360
           L N    S  K  L   N  ++ +  +Q E+ A A+    +Y+ E  +  +P     E+ 
Sbjct: 330 LGN---CSIFKSLLGMGNRGSKIIVTTQNEKIADAVGLKKLYKNESQVVPSP-----EAT 389

Query: 361 SSRLITKDAQNLDVSIPQVTECEPDRGYQTIFKLKKLSKQSSSLLFKEYAFRNSPEVQNP 420
               + KD                   +QTIFK+++LSK++S  LFK +AF  + E Q P
Sbjct: 390 KPSDVNKDNMK----------------HQTIFKVERLSKENSLSLFKVHAFTETQEAQIP 449

Query: 421 ELTKIVDQLLEKCMGVPLAIKCLGSLLSSKTSIAEWKNIEKKLMPQEKKEDG---ILHVL 480
            LTKI + + +KC GVPLAIKCLG LLS KTSIAEW  +  KL   E++EDG   IL  L
Sbjct: 450 NLTKIQEVIEQKCHGVPLAIKCLGGLLS-KTSIAEWNGVIDKLWEHEEEEDGNKSILPTL 509

Query: 481 RVCYDQMPSQLKPCFLYCSQLPNDRIFSSNDMIQLWMANGLLHSPEEKNLAMENIGKKYF 540
           R+CYDQMPS L+ CFLYCSQL  DRI SSND+IQLW+A+ LL  P+E  L++E IG+ YF
Sbjct: 510 RLCYDQMPSHLQRCFLYCSQLKKDRILSSNDVIQLWIASDLL--PKENYLSLEKIGENYF 569

Query: 541 MELWSRCFIQEIEEHGLGYWIKLHPLIQKLAHKITQEQSEGSWGNNHSKEITEIRSIAFQ 600
            EL SRCF+QE+EE+G GYW KLHPLI+KLA  +TQ+Q           E+T+ +SIAF 
Sbjct: 570 KELCSRCFLQELEEYGFGYWFKLHPLIEKLARLLTQKQVF---------EVTKTQSIAFT 629

Query: 601 ERNMVLPNASLTEKCIWRYKCLRLLYLGNADLQEIPNSIGTLKYLRYLDLHGNQKIKHLP 660
            R+ V P+A L   CI ++K LRLL+LGNA+LQ IP+++  L  LRYLDL GN+KIK LP
Sbjct: 630 IRDKVPPSAFLANACIDKFKYLRLLHLGNANLQGIPSAVENLVQLRYLDLQGNKKIKRLP 689

Query: 661 DSICDLQSLQTLILDSCSALEDLPKDIRNLISLRYLWVTTNKLHLHKNGVGTMTSLRFLA 720
           +SI  L++LQTLIL SCSAL++LP DIR L +LRYLWVT N L LHKNGVGTMTSLRFLA
Sbjct: 690 NSIFKLKNLQTLILASCSALKELPNDIRQLTNLRYLWVTANNLRLHKNGVGTMTSLRFLA 749

Query: 721 IGGCKNLENLFERPDCLAGLETLMIYNCNTLKLLPNEMRYLKSLQNLMIWRCKQLTLNLE 780
           IGGC+NL++LF++P CL  LETLMIY+C TLK LPNE+  L SL+NL+IW CK+LTL L+
Sbjct: 750 IGGCQNLQDLFKKPSCLVRLETLMIYDCKTLKSLPNEIGSLISLKNLVIWSCKKLTLTLK 786

Query: 781 EVEFKLQRFTVKELPKVESLPRWLENSIETLRTLQIINCPIRLMELPVDKKYRALEIFLI 840
            VEF+LQRFT++ELP V+ LP W +   ETLR L+II+CPI   +  V K Y++LE F I
Sbjct: 810 GVEFRLQRFTIRELPIVKKLPEWTQRFTETLRVLEIIDCPIEWND-DVLKSYKSLERFSI 786

Query: 841 HGSVRF-DTMPDYDFEHRN 852
           HG+VR  + +  Y+ ++RN
Sbjct: 870 HGAVRTKNQIGGYNIDYRN 786

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
RGA1_SOLBU1.3e-4536.20Putative disease resistance protein RGA1 OS=Solanum bulbocastanum GN=RGA1 PE=2 S... [more]
RGA3_SOLBU5.4e-4434.72Putative disease resistance protein RGA3 OS=Solanum bulbocastanum GN=RGA3 PE=2 S... [more]
RGA4_SOLBU7.1e-4435.40Putative disease resistance protein RGA4 OS=Solanum bulbocastanum GN=RGA4 PE=2 S... [more]
RGA2_SOLBU7.4e-4135.84Disease resistance protein RGA2 OS=Solanum bulbocastanum GN=RGA2 PE=1 SV=1[more]
R13L1_ARATH3.0e-3430.17Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana GN=RPPL... [more]
Match NameE-valueIdentityDescription
A0A0A0LBH9_CUCSA0.0e+0080.46Uncharacterized protein OS=Cucumis sativus GN=Csa_3G815400 PE=3 SV=1[more]
A0A0A0LF99_CUCSA5.4e-19247.50Uncharacterized protein OS=Cucumis sativus GN=Csa_3G814390 PE=3 SV=1[more]
A0A0A0LEM8_CUCSA8.0e-14352.57Uncharacterized protein OS=Cucumis sativus GN=Csa_3G814400 PE=3 SV=1[more]
A0A0A0LEM8_CUCSA3.5e-7448.73Uncharacterized protein OS=Cucumis sativus GN=Csa_3G814400 PE=3 SV=1[more]
A0A0A0LBX7_CUCSA1.4e+0233.77Uncharacterized protein OS=Cucumis sativus GN=Csa_3G822460 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G14470.11.7e-3530.17 NB-ARC domain-containing disease resistance protein[more]
AT4G27220.17.6e-2827.61 NB-ARC domain-containing disease resistance protein[more]
AT3G14460.12.4e-2625.00 LRR and NB-ARC domains-containing disease resistance protein[more]
AT1G58390.12.5e-2327.42 Disease resistance protein (CC-NBS-LRR class) family[more]
AT1G59620.12.1e-2224.50 Disease resistance protein (CC-NBS-LRR class) family[more]
Match NameE-valueIdentityDescription
gi|659084955|ref|XP_008443163.1|0.0e+00100.00PREDICTED: probable disease resistance protein At1g59620 isoform X1 [Cucumis mel... [more]
gi|659084957|ref|XP_008443164.1|0.0e+0098.81PREDICTED: probable disease resistance protein At1g59620 isoform X2 [Cucumis mel... [more]
gi|700204242|gb|KGN59375.1|0.0e+0080.46hypothetical protein Csa_3G815400 [Cucumis sativus][more]
gi|778685015|ref|XP_004136758.2|0.0e+0080.46PREDICTED: putative disease resistance protein RGA1 [Cucumis sativus][more]
gi|700204239|gb|KGN59372.1|7.8e-19247.50hypothetical protein Csa_3G814390 [Cucumis sativus][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001611Leu-rich_rpt
IPR002182NB-ARC
IPR027417P-loop_NTPase
Vocabulary: Molecular Function
TermDefinition
GO:0005515protein binding
GO:0043531ADP binding
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
biological_process GO:0006952 defense response
cellular_component GO:0005575 cellular_component
molecular_function GO:0043531 ADP binding
molecular_function GO:0005515 protein binding
This gene is associated with the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
MU55863melon EST collection version 4.0transcribed_cluster

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO3C009695T1MELO3C009695T1mRNA


The following transcribed_cluster feature(s) are associated with this gene:

Feature NameUnique NameType
MU55863MU55863transcribed_cluster


Analysis Name: InterPro Annotations of melon
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001611Leucine-rich repeatPFAMPF13855LRR_8coord: 615..670
score: 1.
IPR002182NB-ARCPFAMPF00931NB-ARCcoord: 385..516
score: 2.9E-23coord: 156..294
score: 7.7
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3DG3DSA:3.40.50.300coord: 157..282
score: 2.
IPR027417P-loop containing nucleoside triphosphate hydrolaseunknownSSF52540P-loop containing nucleoside triphosphate hydrolasescoord: 384..491
score: 4.51E-18coord: 147..293
score: 1.46
NoneNo IPR availableunknownCoilCoilcoord: 11..31
scor
NoneNo IPR availablePRINTSPR00364DISEASERSISTcoord: 158..173
score: 2.3E-10coord: 657..673
score: 2.3E-10coord: 429..443
score: 2.3
NoneNo IPR availablePANTHERPTHR23155LEUCINE-RICH REPEAT-CONTAINING PROTEINcoord: 372..847
score: 3.0E-133coord: 1..276
score: 3.0E
NoneNo IPR availablePANTHERPTHR23155:SF640SUBFAMILY NOT NAMEDcoord: 372..847
score: 3.0E-133coord: 1..276
score: 3.0E