BLAST of MELO3C009693 vs. Swiss-Prot
Match:
RGA1_SOLBU (Putative disease resistance protein RGA1 OS=Solanum bulbocastanum GN=RGA1 PE=2 SV=2)
HSP 1 Score: 228.8 bits (582), Expect = 2.1e-58
Identity = 185/636 (29.09%), Postives = 310/636 (48.74%), Query Frame = 1
Query: 7 SLKTILMDAEKEEYSHH-LNDWLQELQNVFSQIEGLLYEFNGKVKK--QEATGKW-VFVL 66
+++ +L DA++++ + L +WLQ+L +++ +L E+ K + Q G++ V+
Sbjct: 40 TIQAVLEDAQEKQLNDKPLENWLQKLNAATYEVDDILDEYKTKATRFLQSEYGRYHPKVI 99
Query: 67 SFNSSRIEQTKKMMKLCDDLDEIASQMYDFNLTNMETTHSFLSATEVSTRLMKPSWQLLY 126
F R + K+M ++ L+ IA + F+L + + E + L +P +Y
Sbjct: 100 PF---RHKVGKRMDQVMKKLNAIAEERKKFHLQE-KIIERQAATRETGSVLTEPQ---VY 159
Query: 127 PLTNAPKVFHDKRYSNFLDHFKKSTLGFFHIVGEPGIGKTTLAKFFYNDPEVVKTFPSRL 186
+ D K S L I+G G+GKTTL++ +ND V + F ++
Sbjct: 160 GRDKEKDEIVKILINTASDAQKLSVLP---ILGMGGLGKTTLSQMVFNDQRVTERFYPKI 219
Query: 187 WICVKEEFDPRRLIKEMLDFSHCQATYDNLTEKQLCFAVQQFLRDKTFLLVFQDISIKNL 246
WIC+ ++F+ +RLIK +++ ++ D + L +Q+ L K + LV D+ ++
Sbjct: 220 WICISDDFNEKRLIKAIVESIEGKSLSD-MDLAPLQKKLQELLNGKRYFLVLDDVWNEDQ 279
Query: 247 GDCSIFKSLLGMGNPGSKIIVTTQNEEIAEAIRLGKLCKNESQEVPSPEATEPSDVNNDE 306
+ +++L +G G+ ++ TT+ E++ +G L +P
Sbjct: 280 HKWANLRAVLKVGASGAFVLTTTRLEKVGSI--MGTL--------------QP------- 339
Query: 307 AYKDNMTYQKIFEVERLSKENSLSLFKVHAFTETQEAQIPNLTKIQEVIEQKCHGVPLAI 366
+E+ LS E+ LF AF +E PNL I + I +KC GVPLA
Sbjct: 340 -----------YELSNLSPEDCWFLFMQRAFGHQEEIN-PNLMAIGKEIVKKCGGVPLAA 399
Query: 367 KCLGGLLS-KTSIAEWNGVIDK-LWEHEEGEDGNKSILPVLRLCYDRMPSHLQRCFLYCS 426
K LGG+L K EW V D +W + E SILP LRL Y +P L++CF+YC+
Sbjct: 400 KTLGGILRFKREEREWEHVRDSPIWNLPQDE---SSILPALRLSYHHLPLDLRQCFVYCA 459
Query: 427 QLTKDRILSSNDVIQLWIASDLLPKENYLSLEKIGENYFKELCSRCFLQELEEYGLGYWF 486
KD ++ ++I W+A L + L LE +G + EL R F QE+E +F
Sbjct: 460 VFPKDTKMAKENLIAFWMAHGFLLSKGNLELEDVGNEVWNELYLRSFFQEIEVESGKTYF 519
Query: 487 KLHPLIEKLARIL-----TQKQVFPV-ANTKCIAFTVRDKVPPSAFLANTCIDKFKYIRL 546
K+H LI LA L + + + AN ++ S++ + + + KF +R+
Sbjct: 520 KMHDLIHDLATSLFSANTSSSNIREINANYDGYMMSIGFAEVVSSY-SPSLLQKFVSLRV 579
Query: 547 LYLGNANLREIPNAVEKLVQLRYLDLQGNKRLKRLPNSIFNLKNLQTLILASCSALEELP 606
L L N+NL ++P+++ LV LRYLDL GN R++ LP + L+NLQTL L C +L LP
Sbjct: 580 LNLRNSNLNQLPSSIGDLVHLRYLDLSGNFRIRNLPKRLCKLQNLQTLDLHYCDSLSCLP 625
Query: 607 NHIKQLINLRYLWVTANNLRLHKNGVGTMTSLRFLA 631
+L +LR L + +L +G +T L+ L+
Sbjct: 640 KQTSKLGSLRNLLLDGCSLTSTPPRIGLLTCLKSLS 625
HSP 2 Score: 43.1 bits (100), Expect = 1.6e-02
Identity = 36/118 (30.51%), Postives = 49/118 (41.53%), Query Frame = 1
Query: 539 NANLREIPNAVEK-LVQLRYLDLQGNKRLKRLPNSIFNLKNLQTLILASCSALEELPNHI 598
N +P + K L L+YL + + LK LP S+ +L L++L C ALE LP
Sbjct: 853 NVEATSLPEEMFKSLANLKYLKISFFRNLKELPTSLASLNALKSLKFEFCDALESLP--- 912
Query: 599 KQLINLRYLWVTANNLRLHKNGVGTMTSLRFLAIGGCENLQDLFEQPSCLVRLETLMI 656
+ GV +TSL L++ C L+ L E L L TL I
Sbjct: 913 -------------------EEGVKGLTSLTELSVSNCMMLKCLPEGLQHLTALTTLTI 948
BLAST of MELO3C009693 vs. Swiss-Prot
Match:
RGA4_SOLBU (Putative disease resistance protein RGA4 OS=Solanum bulbocastanum GN=RGA4 PE=2 SV=1)
HSP 1 Score: 221.5 bits (563), Expect = 3.3e-56
Identity = 182/640 (28.44%), Postives = 296/640 (46.25%), Query Frame = 1
Query: 7 SLKTILMDA-EKEEYSHHLNDWLQELQNVFSQIEGLLYEFNGKVKKQEATGKWVFVLSFN 66
+++ +L DA EK+ + +WLQ+L + +++ +L E + + E + +
Sbjct: 40 TIQAVLQDAQEKQLKDKAIENWLQKLNSAAYEVDDILGECKNEAIRFEQSRLGFYHPGII 99
Query: 67 SSRIEQTKKMMKLCDDLDEIASQMYDFNLTNMETTHSFLSATEVSTRLMKPSWQLLYPLT 126
+ R + ++M ++ + LD I+ + F+ T +AT + ++
Sbjct: 100 NFRHKIGRRMKEIMEKLDAISEERRKFHFLEKITERQAAAATRETGFVL----------- 159
Query: 127 NAPKVF-HDKRYSNFLDHFKKST-----LGFFHIVGEPGIGKTTLAKFFYNDPEVVKTFP 186
PKV+ DK + + L F I+G G+GKTTLA+ +ND V K F
Sbjct: 160 TEPKVYGRDKEEDEIVKILINNVNVAEELPVFPIIGMGGLGKTTLAQMIFNDERVTKHFN 219
Query: 187 SRLWICVKEEFDPRRLIKEML-DFSHCQATYDNLTEKQLCFAVQQFLRDKTFLLVFQDIS 246
++W+CV ++FD +RLIK ++ + ++L Q +Q+ L K +LLV D+
Sbjct: 220 PKIWVCVSDDFDEKRLIKTIIGNIERSSPHVEDLASFQK--KLQELLNGKRYLLVLDDV- 279
Query: 247 IKNLGDCSIFKSLLGMGNPGSKIIVTTQNEEIAEAIRLGKLCKNESQEVPSPEATEPSDV 306
N+++ + +L + ++ T V
Sbjct: 280 ---------------------------WNDDLEKWAKLRAVLTVGARGASILATTRLEKV 339
Query: 307 NNDEAYKDNMTYQKIFEVERLSKENSLSLFKVHAFTETQEAQIPNLTKIQEVIEQKCHGV 366
+ M + + + LS +SL LF AF + +EA PNL I + I +KC GV
Sbjct: 340 GSI------MGTLQPYHLSNLSPHDSLLLFMQRAFGQQKEAN-PNLVAIGKEIVKKCGGV 399
Query: 367 PLAIKCLGGLLS-KTSIAEWNGVID-KLWEHEEGEDGNKSILPVLRLCYDRMPSHLQRCF 426
PLA K LGGLL K +EW V D ++W + E SILP LRL Y +P L++CF
Sbjct: 400 PLAAKTLGGLLRFKREESEWEHVRDNEIWSLPQDES---SILPALRLSYHHLPLDLRQCF 459
Query: 427 LYCSQLTKDRILSSNDVIQLWIASDLLPKENYLSLEKIGENYFKELCSRCFLQELEEYGL 486
YC+ KD + ++I LW+A L + L LE +G + EL R F QE+E
Sbjct: 460 AYCAVFPKDTKMIKENLITLWMAHGFLLSKGNLELEDVGNEVWNELYLRSFFQEIEAKSG 519
Query: 487 GYWFKLHPLIEKLARILTQKQV----FPVANTKCIAFTVRDKVPPSAFLAN---TCIDKF 546
+FK+H LI LA L N K TV + +A +++ + + KF
Sbjct: 520 NTYFKIHDLIHDLATSLFSASASCGNIREINVKDYKHTV--SIGFAAVVSSYSPSLLKKF 579
Query: 547 KYIRLLYLGNANLREIPNAVEKLVQLRYLDLQGNKRLKRLPNSIFNLKNLQTLILASCSA 606
+R+L L + L ++P+++ L+ LRYLDL N + LP + L+NLQTL + +C +
Sbjct: 580 VSLRVLNLSYSKLEQLPSSIGDLLHLRYLDLSCN-NFRSLPERLCKLQNLQTLDVHNCYS 625
Query: 607 LEELPNHIKQLINLRYLWVTANNLRLHKNGVGTMTSLRFL 630
L LP +L +LR+L V L +G +T L+ L
Sbjct: 640 LNCLPKQTSKLSSLRHLVVDGCPLTSTPPRIGLLTCLKTL 625
HSP 2 Score: 50.1 bits (118), Expect = 1.3e-04
Identity = 42/174 (24.14%), Postives = 77/174 (44.25%), Query Frame = 1
Query: 534 LLYLGNANLREIPNAVEKLVQLRY----LDLQGNKRLKRLPNSIFNLKNLQTLILASCSA 593
LL GN L ++ N V + LR ++ + ++ + I +L ASC
Sbjct: 435 LLSKGNLELEDVGNEVWNELYLRSFFQEIEAKSGNTYFKIHDLIHDLATSLFSASASCGN 494
Query: 594 LEEL---------------------PNHIKQLINLRYLWVTANNLRLHKNGVGTMTSLRF 653
+ E+ P+ +K+ ++LR L ++ + L + +G + LR+
Sbjct: 495 IREINVKDYKHTVSIGFAAVVSSYSPSLLKKFVSLRVLNLSYSKLEQLPSSIGDLLHLRY 554
Query: 654 LAIGGCENLQDLFEQPSCLVRLETLMIYDSKTLKLLPNEIGALISLKNLVIWSC 683
L + C N + L E+ L L+TL +++ +L LP + L SL++LV+ C
Sbjct: 555 LDLS-CNNFRSLPERLCKLQNLQTLDVHNCYSLNCLPKQTSKLSSLRHLVVDGC 607
HSP 3 Score: 42.7 bits (99), Expect = 2.1e-02
Identity = 28/89 (31.46%), Postives = 43/89 (48.31%), Query Frame = 1
Query: 552 LVQLRYLDLQGNKRLKRLPNSIFNLKNLQTLILASCSALEELPNH-IKQLINLRYLWVT- 611
L L +L K LK LP S+ +L L+ L + SC +LE P ++ L +L L+V
Sbjct: 877 LTNLEFLSFFDFKNLKDLPTSLTSLNALKRLQIESCDSLESFPEQGLEGLTSLTQLFVKY 936
Query: 612 ANNLRLHKNGVGTMTSLRFLAIGGCENLQ 639
L+ G+ +T+L L + GC ++
Sbjct: 937 CKMLKCLPEGLQHLTALTNLGVSGCPEVE 965
BLAST of MELO3C009693 vs. Swiss-Prot
Match:
RGA2_SOLBU (Disease resistance protein RGA2 OS=Solanum bulbocastanum GN=RGA2 PE=1 SV=1)
HSP 1 Score: 211.5 bits (537), Expect = 3.4e-53
Identity = 180/646 (27.86%), Postives = 309/646 (47.83%), Query Frame = 1
Query: 7 SLKTILMDAEKEEYSHH-LNDWLQELQNVFSQIEGLLYEFNGKVKK--QEATGKW-VFVL 66
+++ +L DA++++ ++ L +WLQ+L +++ +L E+ K + Q G++ V+
Sbjct: 40 TIQAVLEDAQEKQLNNKPLENWLQKLNAATYEVDDILDEYKTKATRFSQSEYGRYHPKVI 99
Query: 67 SFNSSRIEQTKKMMKLCDDLDEIASQMYDFNLTNM--------ETTHSFLSATEVSTRLM 126
F R + K+M ++ L IA + +F+L T S L+ +V R
Sbjct: 100 PF---RHKVGKRMDQVMKKLKAIAEERKNFHLHEKIVERQAVRRETGSVLTEPQVYGRDK 159
Query: 127 KPSWQLLYPLTNAPKVFHDKRYSNFLDHFKKSTLGFFHIVGEPGIGKTTLAKFFYNDPEV 186
+ + + N H L I+G G+GKTTLA+ +ND V
Sbjct: 160 EKDEIVKILINNVSDAQH---------------LSVLPILGMGGLGKTTLAQMVFNDQRV 219
Query: 187 VKTFPSRLWICVKEEFDPRRLIKEMLDFSHCQATYDNLTEKQLCFAVQQFLRDKTFLLVF 246
+ F S++WICV E+FD +RLIK +++ + + L +Q+ L K +LLV
Sbjct: 220 TEHFHSKIWICVSEDFDEKRLIKAIVESIEGRPLLGEMDLAPLQKKLQELLNGKRYLLVL 279
Query: 247 QDISIKNLGDCSIFKSLLGMGNPGSKIIVTTQNEEIAEAIRLGKLCKNESQEVPSPEATE 306
D+ ++ + +++L +G G+ ++ TT+ E++ +G L + E
Sbjct: 280 DDVWNEDQQKWANLRAVLKVGASGASVLTTTRLEKVGSI--MGTL-----------QPYE 339
Query: 307 PSDVNNDEAYKDNMTYQKIFEVERLSKENSLSLFKVHAFTETQEAQIPNLTKIQEVIEQK 366
S+++ ++ + + ++R AF +E PNL I + I +K
Sbjct: 340 LSNLSQEDCW--------LLFMQR-------------AFGHQEEIN-PNLVAIGKEIVKK 399
Query: 367 CHGVPLAIKCLGGLLS-KTSIAEWNGVIDK-LWEHEEGEDGNKSILPVLRLCYDRMPSHL 426
GVPLA K LGG+L K W V D +W + E SILP LRL Y ++P L
Sbjct: 400 SGGVPLAAKTLGGILCFKREERAWEHVRDSPIWNLPQDE---SSILPALRLSYHQLPLDL 459
Query: 427 QRCFLYCSQLTKDRILSSNDVIQLWIASDLLPKENYLSLEKIGENYFKELCSRCFLQELE 486
++CF YC+ KD + +I LW+A L + + LE +G+ +KEL R F QE+E
Sbjct: 460 KQCFAYCAVFPKDAKMEKEKLISLWMAHGFLLSKGNMELEDVGDEVWKELYLRSFFQEIE 519
Query: 487 EYGLGYWFKLHPLIEKLARILTQ--------KQVFPVANTKCIAFTVRDKVPPSAFLANT 546
+FK+H LI LA L +++ + T ++ + V F
Sbjct: 520 VKDGKTYFKMHDLIHDLATSLFSANTSSSNIREINKHSYTHMMSIGFAEVV---FFYTLP 579
Query: 547 CIDKFKYIRLLYLGNANLREIPNAVEKLVQLRYLDLQGNKRLKRLPNSIFNLKNLQTLIL 606
++KF +R+L LG++ ++P+++ LV LRYL+L G+ ++ LP + L+NLQTL L
Sbjct: 580 PLEKFISLRVLNLGDSTFNKLPSSIGDLVHLRYLNLYGS-GMRSLPKQLCKLQNLQTLDL 625
Query: 607 ASCSALEELPNHIKQLINLRYLWVT-ANNLRLHKNGVGTMTSLRFL 630
C+ L LP +L +LR L + + +L +G++T L+ L
Sbjct: 640 QYCTKLCCLPKETSKLGSLRNLLLDGSQSLTCMPPRIGSLTCLKTL 625
HSP 2 Score: 47.8 bits (112), Expect = 6.5e-04
Identity = 32/90 (35.56%), Postives = 46/90 (51.11%), Query Frame = 1
Query: 550 EKLVQLRYLDLQGNKRLKRLPNSIFNLKNLQTLILASCSALEELPNH-IKQLINLRYLWV 609
+ L L+YL + LK LP S+ +L L++L + C ALE LP ++ L +L L+V
Sbjct: 856 KNLANLKYLTISRCNNLKELPTSLASLNALKSLKIQLCCALESLPEEGLEGLSSLTELFV 915
Query: 610 T-ANNLRLHKNGVGTMTSLRFLAIGGCENL 638
N L+ G+ +T+L L I GC L
Sbjct: 916 EHCNMLKCLPEGLQHLTTLTSLKIRGCPQL 945
BLAST of MELO3C009693 vs. Swiss-Prot
Match:
RGA3_SOLBU (Putative disease resistance protein RGA3 OS=Solanum bulbocastanum GN=RGA3 PE=2 SV=2)
HSP 1 Score: 176.8 bits (447), Expect = 9.3e-43
Identity = 180/661 (27.23%), Postives = 291/661 (44.02%), Query Frame = 1
Query: 8 LKTILMDAEKEEYSHH-LNDWLQELQNVFSQIEGLLYEFNGKVKKQEATGKWVFVLSFNS 67
++ +L DA++++ + + +WLQ+L +++ +L + K + A K + ++
Sbjct: 41 IQAVLEDAQEKQLKYKAIKNWLQKLNVAAYEVDDILDD----CKTEAARFKQAVLGRYHP 100
Query: 68 SRI----EQTKKMMKLCDDLDEIASQMYDFNLTNMETTHSFLSATEVSTRLMKPSWQLLY 127
I + K+M ++ + LD IA + +F+L + + + R Q +
Sbjct: 101 RTITFCYKVGKRMKEMMEKLDAIAEERRNFHLDER------IIERQAARR------QTGF 160
Query: 128 PLTNAPKVFHDKRYSNFLDHFKKSTLGFFH------IVGEPGIGKTTLAKFFYNDPEVVK 187
LT PKV+ ++ + + + + + I+G G+GKTTLA+ +ND
Sbjct: 161 VLTE-PKVYGREKEEDEIVKILINNVSYSEEVPVLPILGMGGLGKTTLAQMVFND----- 220
Query: 188 TFPSRLWICVKEEFDPRRLIKEMLDFSHCQATYDNLTEKQLCFAVQQFLRDKTFLLVFQD 247
+ I E + D+ EK+L A+ +
Sbjct: 221 -----------------QRITEHFNLKIWVCVSDDFDEKRLIKAI------------VES 280
Query: 248 ISIKNLGDCSIF---KSLLGMGNPGSKIIVTTQ--NEEIAEAIRLGKLCKNESQEVPSPE 307
I K+LGD + K L + N +V NE+ + L + K +
Sbjct: 281 IEGKSLGDMDLAPLQKKLQELLNGKRYFLVLDDVWNEDQEKWDNLRAVLKIGASGASILI 340
Query: 308 ATEPSDVNNDEAYKDNMTYQKIFEVERLSKENSLSLFKVHAFTETQEAQIPNLTKIQEVI 367
T + + M +++++ LS+E+ LFK AF E P L +I + I
Sbjct: 341 TTRLEKIGSI------MGTLQLYQLSNLSQEDCWLLFKQRAFCHQTETS-PKLMEIGKEI 400
Query: 368 EQKCHGVPLAIKCLGGLLS-KTSIAEWNGVID-KLWEHEEGEDGNKSILPVLRLCYDRMP 427
+KC GVPLA K LGGLL K +EW V D ++W + E+ S+LP LRL Y +P
Sbjct: 401 VKKCGGVPLAAKTLGGLLRFKREESEWEHVRDSEIWNLPQDEN---SVLPALRLSYHHLP 460
Query: 428 SHLQRCFLYCSQLTKDRILSSNDVIQLWIASDLLPKENYLSLEKIGENYFKELCSRCFLQ 487
L++CF YC+ KD + +I LW+A L + + LE +G + EL R F Q
Sbjct: 461 LDLRQCFAYCAVFPKDTKIEKEYLIALWMAHSFLLSKGNMELEDVGNEVWNELYLRSFFQ 520
Query: 488 ELEEYGLGYWFKLHPLIEKLARIL-----------------TQKQVFPVANTK---CIAF 547
E+E +FK+H LI LA + + +F V N K I F
Sbjct: 521 EIEVKSGKTYFKMHDLIHDLATSMFSASASSRSIRQINVKDDEDMMFIVTNYKDMMSIGF 580
Query: 548 T-VRDKVPPSAFLANTCIDKFKYIRLLYLGNANLREIPNAVEKLVQLRYLDLQGNKRLKR 607
+ V PS F +F +R+L L N+ ++P++V LV LRYLDL GNK +
Sbjct: 581 SEVVSSYSPSLF------KRFVSLRVLNLSNSEFEQLPSSVGDLVHLRYLDLSGNK-ICS 633
Query: 608 LPNSIFNLKNLQTLILASCSALEELPNHIKQLINLRYLWVTANNLRLHKNGVGTMTSLRF 630
LP + L+NLQTL L +C +L LP +L +LR L + L +G +T L+
Sbjct: 641 LPKRLCKLQNLQTLDLYNCQSLSCLPKQTSKLCSLRNLVLDHCPLTSMPPRIGLLTCLKT 633
HSP 2 Score: 47.0 bits (110), Expect = 1.1e-03
Identity = 33/90 (36.67%), Postives = 46/90 (51.11%), Query Frame = 1
Query: 550 EKLVQLRYLDLQGNKRLKRLPNSIFNLKNLQTLILASCSALEELPNH-IKQLINLRYLWV 609
+ L L YL + + LK LP S+ +L NL+ L + C ALE LP ++ L +L L+V
Sbjct: 878 KNLENLIYLSVSFLENLKELPTSLASLNNLKCLDIRYCYALESLPEEGLEGLSSLTELFV 937
Query: 610 T-ANNLRLHKNGVGTMTSLRFLAIGGCENL 638
N L+ G+ +T+L L I GC L
Sbjct: 938 EHCNMLKCLPEGLQHLTTLTSLKIRGCPQL 967
BLAST of MELO3C009693 vs. Swiss-Prot
Match:
R13L1_ARATH (Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana GN=RPPL1 PE=3 SV=1)
HSP 1 Score: 158.7 bits (400), Expect = 2.6e-37
Identity = 173/708 (24.44%), Postives = 311/708 (43.93%), Query Frame = 1
Query: 5 MLSLKTILMDAEKEEYSHHLND-WLQELQNVFSQIEGLLYEFNGKVKKQEATGKWVFVLS 64
+L++ +L+DAE+++ ++ + + W+ EL++V E L + EA + S
Sbjct: 46 LLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDI-----ATEALRLNIGAES 105
Query: 65 FNSSRIEQTKKMMKLCDDLD------EIASQMYDFNLTNMETTHSFLSATEVSTRLMKPS 124
+S+R+ Q + M L D LD E + L + + + L E++ + K
Sbjct: 106 SSSNRLRQLRGRMSLGDFLDGNSEHLETRLEKVTIRLERLASQRNILGLKELTAMIPKQR 165
Query: 125 WQLLYPLTNAPKVF---HDK-RYSNFL--DHFKKSTLGFFHIVGEPGIGKTTLAKFFYND 184
L + +VF DK FL ++ K + + IVG G+GKTTL++ YND
Sbjct: 166 LPTT-SLVDESEVFGRDDDKDEIMRFLIPENGKDNGITVVAIVGIGGVGKTTLSQLLYND 225
Query: 185 PEVVKTFPSRLWICVKEEFDPRRLIKEMLDFSHCQATYDNLTEKQLCFAVQQFLRDK--- 244
V F +++W V EEFD ++ K++ Y+++T + F L+ K
Sbjct: 226 QHVRSYFGTKVWAHVSEEFDVFKITKKV---------YESVTSRPCEFTDLDVLQVKLKE 285
Query: 245 -------TFLLVFQDISIKNLGDCSIFKSLLGMGNPGSKIIVTTQNEEIAEAIRLGKLCK 304
FLLV D+ +N D + + GS+I+VTT+++ +A +C
Sbjct: 286 RLTGTGLPFLLVLDDLWNENFADWDLLRQPFIHAAQGSQILVTTRSQRVASI-----MC- 345
Query: 305 NESQEVPSPEATEPSDVNNDEAYKDNMTYQKIFEVERLSKENSLSLFKVHAFTETQEAQI 364
V+N + D + + ++E L+
Sbjct: 346 -------------AVHVHNLQPLSDGDCWSLFMKTVFGNQEPCLN--------------- 405
Query: 365 PNLTKIQEVIEQKCHGVPLAIKCLGGLLS-KTSIAEWNGVI-DKLWEHEEGEDGNKSILP 424
+ + E I KC G+PLA+K LGG+L + + EW V+ ++W+ + ++LP
Sbjct: 406 REIGDLAERIVHKCRGLPLAVKTLGGVLRFEGKVIEWERVLSSRIWDLPADK---SNLLP 465
Query: 425 VLRLCYDRMPSHLQRCFLYCSQLTKDRILSSNDVIQLWIASDLLPK-ENYLSLEKIGENY 484
VLR+ Y +P+HL+RCF YCS K + V+ LW+A L + + +LE++G Y
Sbjct: 466 VLRVSYYYLPAHLKRCFAYCSIFPKGHAFEKDKVVLLWMAEGFLQQTRSSKNLEELGNEY 525
Query: 485 FKELCSRCFLQELEEYGLGYWFKLHPLIEKLARILTQK---------QVFPVANTKCIAF 544
F EL SR LQ+ + + +H I +LA+ + + ++ T+ +++
Sbjct: 526 FSELESRSLLQKTKTR-----YIMHDFINELAQFASGEFSSKFEDGCKLQVSERTRYLSY 585
Query: 545 TVRDKVPPSAFLANTCIDKFKYIRLLYLGNANLREIPNAVEKLVQLRYLDLQGNKRLKRL 604
+ P F A + + K++R +P ++ + LD +++L L
Sbjct: 586 LRDNYAEPMEFEA---LREVKFLRTF---------LPLSLTNSSRSCCLDQMVSEKL--L 645
Query: 605 PNSIFNLKNLQTLILASCSALEELPNHIKQLINLRYLWVTANNLRLHKNGVGTMTSLRFL 664
P L L+ L L+ P+ K + + R+L ++ L + M +L+ L
Sbjct: 646 P----TLTRLRVLSLSHYKIARLPPDFFKNISHARFLDLSRTELEKLPKSLCYMYNLQTL 677
Query: 665 AIGGCENLQDLFEQPSCLVRLETLMIYDSKTLKLLPNEIGALISLKNL 678
+ C +L++L S L+ L L + +K L+ +P G L SL+ L
Sbjct: 706 LLSYCSSLKELPTDISNLINLRYLDLIGTK-LRQMPRRFGRLKSLQTL 677
BLAST of MELO3C009693 vs. TrEMBL
Match:
A0A0A0LF99_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G814390 PE=3 SV=1)
HSP 1 Score: 1349.3 bits (3491), Expect = 0.0e+00
Identity = 677/785 (86.24%), Postives = 718/785 (91.46%), Query Frame = 1
Query: 1 MQYTMLSLKTILMDAEKEEYSHHLNDWLQELQNVFSQIEGLLYEFNGKVKKQEATGKWVF 60
MQYTMLSLKTIL DAEKEEY H LNDWLQ+LQ+VF QIE LLYE N +VKKQEATGKWVF
Sbjct: 26 MQYTMLSLKTILKDAEKEEYRHCLNDWLQKLQSVFLQIEELLYESNREVKKQEATGKWVF 85
Query: 61 VLSFNSSRIEQTKKMMKLCDDLDEIASQMYDFNLTNMETTHSFLSATEVSTRLMKPSWQL 120
+ SFN S+I+QTKKMMKLCDDLDEIAS MY FNLTNMETTHSFLSATEVSTRLMKPSWQL
Sbjct: 86 LPSFNFSQIDQTKKMMKLCDDLDEIASHMYGFNLTNMETTHSFLSATEVSTRLMKPSWQL 145
Query: 121 LYPLTNAPKVFHDKRYSNFLDHFKKSTLGFFHIVGEPGIGKTTLAKFFYNDPEVVKTFPS 180
LY LTNAPKVF DKRY NFLDHFKKST G FHIVGEPGIGKTTLAKFFYN+ +V TFPS
Sbjct: 146 LYSLTNAPKVFQDKRYHNFLDHFKKSTHGLFHIVGEPGIGKTTLAKFFYNN--LVNTFPS 205
Query: 181 RLWICVKEEFDPRRLIKEMLDFSHCQATYDNLTEKQLCFAVQQFLRDKTFLLVFQDISIK 240
RLWICVKEEFDP+RLIKEML FSHCQ T DNLTEKQLCFAVQQFLRDK FL+VFQDISIK
Sbjct: 206 RLWICVKEEFDPQRLIKEMLSFSHCQVTCDNLTEKQLCFAVQQFLRDKKFLIVFQDISIK 265
Query: 241 NLGDCSIFKSLLGMGNPGSKIIVTTQNEEIAEAIRLGKLCKNESQEVPSPEATEPSDVNN 300
NLG+CSIFKSLLGMGN GSKIIVTTQNE+IA+A+ L KL KNESQ VPSPEAT+PSDVN
Sbjct: 266 NLGNCSIFKSLLGMGNRGSKIIVTTQNEKIADAVGLKKLYKNESQVVPSPEATKPSDVN- 325
Query: 301 DEAYKDNMTYQKIFEVERLSKENSLSLFKVHAFTETQEAQIPNLTKIQEVIEQKCHGVPL 360
KDNM +Q IF+VERLSKENSLSLFKVHAFTETQEAQIPNLTKIQEVIEQKCHGVPL
Sbjct: 326 ----KDNMKHQTIFKVERLSKENSLSLFKVHAFTETQEAQIPNLTKIQEVIEQKCHGVPL 385
Query: 361 AIKCLGGLLSKTSIAEWNGVIDKLWEHEEGEDGNKSILPVLRLCYDRMPSHLQRCFLYCS 420
AIKCLGGLLSKTSIAEWNGVIDKLWEHEE EDGNKSILP LRLCYD+MPSHLQRCFLYCS
Sbjct: 386 AIKCLGGLLSKTSIAEWNGVIDKLWEHEEEEDGNKSILPTLRLCYDQMPSHLQRCFLYCS 445
Query: 421 QLTKDRILSSNDVIQLWIASDLLPKENYLSLEKIGENYFKELCSRCFLQELEEYGLGYWF 480
QL KDRILSSNDVIQLWIASDLLPKENYLSLEKIGENYFKELCSRCFLQELEEYG GYWF
Sbjct: 446 QLKKDRILSSNDVIQLWIASDLLPKENYLSLEKIGENYFKELCSRCFLQELEEYGFGYWF 505
Query: 481 KLHPLIEKLARILTQKQVFPVANTKCIAFTVRDKVPPSAFLANTCIDKFKYIRLLYLGNA 540
KLHPLIEKLAR+LTQKQVF V T+ IAFT+RDKVPPSAFLAN CIDKFKY+RLL+LGNA
Sbjct: 506 KLHPLIEKLARLLTQKQVFEVTKTQSIAFTIRDKVPPSAFLANACIDKFKYLRLLHLGNA 565
Query: 541 NLREIPNAVEKLVQLRYLDLQGNKRLKRLPNSIFNLKNLQTLILASCSALEELPNHIKQL 600
NL+ IP+AVE LVQLRYLDLQGNK++KRLPNSIF LKNLQTLILASCSAL+ELPN I+QL
Sbjct: 566 NLQGIPSAVENLVQLRYLDLQGNKKIKRLPNSIFKLKNLQTLILASCSALKELPNDIRQL 625
Query: 601 INLRYLWVTANNLRLHKNGVGTMTSLRFLAIGGCENLQDLFEQPSCLVRLETLMIYDSKT 660
NLRYLWVTANNLRLHKNGVGTMTSLRFLAIGGC+NLQDLF++PSCLVRLETLMIYD KT
Sbjct: 626 TNLRYLWVTANNLRLHKNGVGTMTSLRFLAIGGCQNLQDLFKKPSCLVRLETLMIYDCKT 685
Query: 661 LKLLPNEIGALISLKNLVIWSCKQLTLTLKGVEFRLRRFTIRELPRVRKLPEWIQRFTES 720
LK LPNEIG+LISLKNLVIWSCK+LTLTLKGVEFRL+RFTIRELP V+KLPEW QRFTE+
Sbjct: 686 LKSLPNEIGSLISLKNLVIWSCKKLTLTLKGVEFRLQRFTIRELPIVKKLPEWTQRFTET 745
Query: 721 LRVLEIIDCPIELKDDEFKSYKSLERLSIHGAVRIKNLNGEYCIDFQNSVRRREVTKEMK 780
LRVLEIIDCPIE DD KSYKSLER SIHGAVR KN G Y ID++N VR R+V KE+K
Sbjct: 746 LRVLEIIDCPIEWNDDVLKSYKSLERFSIHGAVRTKNQIGGYNIDYRNFVRSRKVKKEVK 803
Query: 781 TCYYY 786
TC YY
Sbjct: 806 TCVYY 803
BLAST of MELO3C009693 vs. TrEMBL
Match:
A0A0A0LEM8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G814400 PE=3 SV=1)
HSP 1 Score: 889.4 bits (2297), Expect = 3.1e-255
Identity = 485/790 (61.39%), Postives = 595/790 (75.32%), Query Frame = 1
Query: 1 MQYTMLSLKTILMDAE-KEEYSHHLNDWLQELQNVFSQIEGLLYEFNGKVKKQEATGKWV 60
++YTMLSLK+ILMDAE K+E S L +WL+ELQNVFSQIEG + E K + E GK V
Sbjct: 26 IEYTMLSLKSILMDAEEKQEQSRGLQNWLEELQNVFSQIEGFIDEH--KEEAYEGIGKQV 85
Query: 61 FV-LSFNSSRIEQTKKMMKLCDDLDEIASQMYDFNLTNM-------ETTHSFLSATEVST 120
S +S++I +T KM KL D L+E+A++MY+FNLT ETT+SFL+ATEVST
Sbjct: 86 LAPFSCSSNQIARTWKMEKLFDHLNEVAAKMYEFNLTERHTGAIKTETTNSFLTATEVST 145
Query: 121 RLMKPSWQLLYPLTNAPKVFHDKRYSNFLDHFKKSTLGFFHIVGEPGIGKTTLAKFFYND 180
RLMKPSW++LYPLTNAPK + D+RY L+ FK TLGFFHIVGE GIGK+TLAKF YND
Sbjct: 146 RLMKPSWKVLYPLTNAPKFYQDERYRKILNDFKNPTLGFFHIVGEAGIGKSTLAKFIYND 205
Query: 181 PEVVKTFPSRLWICVKEEFDPRRLIKEMLDFSHCQATYDNLTEKQLCFAVQQFLRDKTFL 240
PEV FPSRLW+CVKEEFD +RL+KE+L+FS+ AT DNLT K LC Q+LR++TFL
Sbjct: 206 PEVEGMFPSRLWVCVKEEFDTQRLMKEILNFSYSPATCDNLTTK-LC-PTDQYLRERTFL 265
Query: 241 LVFQDISIKNLGDCSIFKSLLGMGNPGSKIIVTTQNEEIAEAIRLGKLCK-------NES 300
LVFQD+SIKNL +CS+F SLL MG PGSKIIVTTQNEEIA AI L + K N S
Sbjct: 266 LVFQDLSIKNLDNCSLFTSLLMMGKPGSKIIVTTQNEEIANAIELTMIYKVGQQSEQNRS 325
Query: 301 QEVPSPEATEPSDVNNDEAY---------KDNMTYQKIFEVERLSKENSLSLFKVHAFT- 360
Q E ++VNN + + ++ Q IF+V+RLS+++SLSLFK +A T
Sbjct: 326 QTALDTVTKETANVNNADQFVQANPLGKIDQSIPSQTIFKVKRLSEKDSLSLFKDYASTY 385
Query: 361 ETQEAQIPNLTKIQEVIEQKCHGVPLAIKCLGGLLSKTSIA-EWNGVIDKLWEHEEGEDG 420
E E I K KC+G+PLAIKCLG +LS A +W + D E ++G++
Sbjct: 386 EGNEKDIMKTLK-------KCNGIPLAIKCLGSMLSLGPPATKW--MEDN--ERQKGDNE 445
Query: 421 NKSILPVLRLCYDRMPSHLQRCFLYCSQLTKDRILSSNDVIQLWIASDLL--PKENYLSL 480
+ S +L+LCY+ MPSHL+RCFLYCSQL D ILSSNDVIQLW+A+ LL +ENYLSL
Sbjct: 446 SSSTFSILKLCYNEMPSHLKRCFLYCSQLPNDSILSSNDVIQLWMANGLLRSRQENYLSL 505
Query: 481 EKIGENYFKELCSRCFLQELEEYGLGYWFKLHPLIEKLARILTQK-----QVFPVANTKC 540
E IGE YFKELCSRCFLQ++EEYGLGYWFK+HPLI +LAR++ ++ + PV N
Sbjct: 506 EDIGEIYFKELCSRCFLQDVEEYGLGYWFKMHPLIRELARLVQKRTKDLISIKPVTNVTS 565
Query: 541 IAFTVRDKVPPSAFLANTCIDKFKYIRLLYLGNANLREIPNAVEKLVQLRYLDLQGNKRL 600
IAF VRD+VP S+FLA CI KF+++RLLYLG+ +L+EIPN +E L L YLDLQGNK +
Sbjct: 566 IAFPVRDEVPSSSFLAEKCISKFQHLRLLYLGHTDLQEIPNTIETLNHLTYLDLQGNKNI 625
Query: 601 KRLPNSIFNLKNLQTLILASCSALEELPNHIKQLINLRYLWVTANNLRLHKNGVGTMTSL 660
KRLPN+I NL++LQTLILASCSALEELP I +L NLRYLWVT+N LRLHKNGVGTMTSL
Sbjct: 626 KRLPNAICNLQHLQTLILASCSALEELPKDICKLSNLRYLWVTSNKLRLHKNGVGTMTSL 685
Query: 661 RFLAIGGCENLQDLFEQPSCLVRLETLMIYDSKTLKLLPNEIGALISLKNLVIWSCKQLT 720
RFLAIGGC+ LQDLFE+PSCLVRLETLMIYD +L+LLPNE+G+LISL+NLVIWSCKQLT
Sbjct: 686 RFLAIGGCDKLQDLFERPSCLVRLETLMIYDCNSLQLLPNEMGSLISLQNLVIWSCKQLT 745
Query: 721 LT-LKGVEFRLRRFTIRELPRVRKLPEWIQRFTESLRVLEIIDCPIELKDDEFKSYKSLE 756
L L+ V+F L+RFTIRELP V KLPEW+QR TE+LRVLEIIDCPI+++++ K YK++E
Sbjct: 746 LKGLEKVDFSLQRFTIRELPEVNKLPEWLQRSTETLRVLEIIDCPIKVEEEGIKMYKAVE 800
BLAST of MELO3C009693 vs. TrEMBL
Match:
A0A0A0LBH9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G815400 PE=3 SV=1)
HSP 1 Score: 530.8 bits (1366), Expect = 2.8e-147
Identity = 293/545 (53.76%), Postives = 381/545 (69.91%), Query Frame = 1
Query: 246 SIFKSLLGMGNPGSKIIVTTQNEEIAEAIRLGKLCKNESQ---EVPSPEATEPSDV---- 305
S+ K+ PGS+I T + IA I L E EVP P+ TE +++
Sbjct: 339 SLTKNTQNQAVPGSQIEETA--DAIANKIYEHSLSLTEDAQNLEVPIPQVTEDTNIGLGI 398
Query: 306 NND--EAYKDNMTYQKIFEVERLSKENSLSLFKVHAFTETQEAQIPNLTKIQEVIEQKCH 365
N D +D+ YQ IF++E+LSK++S LFK +AF QE + P LTKI + + +KC
Sbjct: 399 NPDIPPIKQDDTEYQTIFKLEKLSKQSSSRLFKEYAFRNRQEVENPELTKIVDQLLEKCM 458
Query: 366 GVPLAIKCLGGLLS-KTSIAEWNGVIDKLWEHEEGEDGNKSILPVLRLCYDRMPSHLQRC 425
GVPLAIKCLG LLS +TSIA+W + +KL E+ E+G IL VLR+CYD+MPS L+ C
Sbjct: 459 GVPLAIKCLGSLLSSETSIAKWKNIEEKLSLQEKKENG---ILHVLRVCYDQMPSQLKPC 518
Query: 426 FLYCSQLTKDRILSSNDVIQLWIASDLL--PKENYLSLEKIGENYFKELCSRCFLQELEE 485
FL+CSQL DRI+SSND+IQLW+A+ LL P+E ++E IGE YF EL SR F+QE+EE
Sbjct: 519 FLHCSQLPNDRIISSNDMIQLWMANGLLHSPEEKNSTMENIGEKYFMELWSRYFIQEIEE 578
Query: 486 YGLGYWFKLHPLIEKLARILTQKQVF---------PVANTKCIAFTVRDKVPPSAFLANT 545
+GLGYW KLHPLI+KLA +TQ+Q V + IAF R+ V P+A L
Sbjct: 579 HGLGYWIKLHPLIQKLAHKITQEQSEGSGGNNHPKEVTEIRSIAFQERNMVLPNASLTEK 638
Query: 546 CIDKFKYIRLLYLGNANLREIPNAVEKLVQLRYLDLQGNKRLKRLPNSIFNLKNLQTLIL 605
CI K+K +RLLYL NA+L+EIPN++ L LRYLDL GN ++K LPNSI NL++LQTLIL
Sbjct: 639 CIWKYKGLRLLYLSNADLQEIPNSIGTLKYLRYLDLHGNTKIKHLPNSICNLQSLQTLIL 698
Query: 606 ASCSALEELPNHIKQLINLRYLWVTANNLRLHKNGVGTMTSLRFLAIGGCENLQDLFEQP 665
SCSALE+LP I+ LI+LRYLWVT N LRL KNGVGTM SLRFLAIGGC NL++LFE+P
Sbjct: 699 GSCSALEDLPKDIRNLISLRYLWVTTNKLRLDKNGVGTMNSLRFLAIGGCNNLENLFERP 758
Query: 666 SCLVRLETLMIYDSKTLKLLPNEIGALISLKNLVIWSCKQLTLTLKGVEFRLRRFTIREL 725
CL LETLMIY+ TLKLLP+E+ L SL+NL+IWSCKQLTL LK VEF+L+RFTI+EL
Sbjct: 759 DCLAGLETLMIYNCTTLKLLPDEMRYLKSLQNLMIWSCKQLTLNLKEVEFKLQRFTIKEL 818
Query: 726 PRVRKLPEWIQRFTESLRVLEIIDCPIELKDDE-FKSYKSLERLSIHGAVRIKNLNGEYC 769
PRV +LP+W++ E+LR L+II+CPI + + + + Y+++E I+GAVR + Y
Sbjct: 819 PRVERLPQWLENSAETLRTLQIINCPIRIMERQGIEKYEAVENTIIYGAVRFEMAPPGYD 878
BLAST of MELO3C009693 vs. TrEMBL
Match:
A0A0A0LBH9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G815400 PE=3 SV=1)
HSP 1 Score: 235.0 bits (598), Expect = 3.2e-58
Identity = 132/290 (45.52%), Postives = 192/290 (66.21%), Query Frame = 1
Query: 1 MQYTMLSLKTILMDAE-KEEYSHHLNDWLQELQNVFSQIEGLLYEFNGKVKKQEATGKWV 60
++Y M SLK +L+DAE K+E + L +WL+ELQNVF Q+E + EF ++ KQ+ GK V
Sbjct: 26 IEYAMSSLKAVLLDAEEKQEQNQRLQNWLKELQNVFYQVEDSIDEFKWEIFKQKDIGKQV 85
Query: 61 FVL-----SFNSSRIEQTKKMMKLCDDLDEIASQMYDFNLT-------NMETTHSFLSAT 120
++++++Q K ++CD+L+ IA++MY+F+L +METTH+F SA+
Sbjct: 86 LAPFSCSNQISANKLKQKCKRKQVCDELNNIATRMYEFHLKVKHIDSISMETTHTFPSAS 145
Query: 121 EVSTRLMKPSWQLLYPLTNAPKVFHDKRYSNFLDHFKKSTLGFFHIVGEPGIGKTTLAKF 180
E+S R +KPSWQLLYPL +A + + D+ Y L+ F + T FHIVGE GIGK+T+A+F
Sbjct: 146 EISIRHLKPSWQLLYPLIDASRTY-DEIYDGILNVFNECT-HVFHIVGEAGIGKSTVARF 205
Query: 181 FYNDPEVVKTFPSRLWICVKEEFDPRRLIKEMLDFSHCQATYDNLTEKQLCFAVQQFLRD 240
YN VV + SR W+CV+E F+ RL+KE+ + + ++LT +QL V++ L+
Sbjct: 206 LYNHNNVVGKYTSRYWVCVEEGFNKHRLVKEVYSHADNKEICEDLTTEQLLSKVKRLLKV 265
Query: 241 KTFLLVFQDISIKNLGDCS--IFKSLLGMGNPGSKIIVTTQNEEIAEAIR 276
+TFLLVFQD+SI NL D S + LL MG GSKIIVTTQ EEIA ++
Sbjct: 266 ETFLLVFQDLSITNLKDRSPLVLNELLEMGQHGSKIIVTTQTEEIANYLQ 313
HSP 2 Score: 344.4 bits (882), Expect = 3.8e-91
Identity = 273/823 (33.17%), Postives = 409/823 (49.70%), Query Frame = 1
Query: 1 MQYTMLSLKTILMDAE-KEEYSHHLNDWLQELQNVFSQIEGLLYEFNGKVKKQEA----- 60
+Q TM ++K +L+DAE K+ ++ + WL++L++VF E LL EF ++E
Sbjct: 37 LQRTMSTIKDVLLDAEQKQAHNQQIRSWLRQLKDVFLDAEDLLDEFECDALRREVVETFH 96
Query: 61 --TGKWVFVLSFNSS---RIEQTKKMMKLCDDLDEIASQMYDFN-LTNME---------- 120
TGK S ++ R+ +M ++ + LDE+ S F+ LT+++
Sbjct: 97 GTTGKVRRFFSRSNPIAFRLRVGHEMKEIRERLDELKSNKAIFDSLTSIDHHGGGGEHHE 156
Query: 121 ---TTHSFLSATEVSTRLMKPSWQLLYPLTNAPKVFHDKRYSNFLDHFKKSTLGFFHIVG 180
THSF+ A++V R + Q++ L D + N + IVG
Sbjct: 157 RVNVTHSFVRASKVIGRESEKK-QIINLLMEQGD---DNQSGN-------GNVSVIPIVG 216
Query: 181 EPGIGKTTLAKFFYNDPEVVKTFPSRLWICVKEEFDPRRLIKEMLDFSHCQATYDNLTEK 240
G+GKTTLAK Y+D VV F R+W+ V +F+ RLIK +L + D L+
Sbjct: 217 IGGLGKTTLAKLVYDDEGVVGHFEKRMWVSVSVDFEITRLIKMILSSASDTEMSDKLSLD 276
Query: 241 QLCFAVQQFLRDKTFLLVFQDISIKNLGDCSIFKSLLGMG-NPGSKIIVTTQNEEIAEAI 300
QL ++ L+DK FLLV D+ ++ S + LL G GSKI+VTT+N +AE +
Sbjct: 277 QLQGRLRHALKDKKFLLVLDDVWNEDSIKWSELRDLLIEGAKSGSKILVTTRNTWVAEMM 336
Query: 301 RLGKLCKNESQEVPSPEATEPSDVNNDEAYKDNMTYQKIFEVERLSKENSLSLFKVHAFT 360
T P+ +N + LS E+ LSLF AF
Sbjct: 337 -----------------GTIPTSINLEF----------------LSFEDCLSLFVECAFK 396
Query: 361 ETQEAQIPNLTKIQEVIEQKCHGVPLAIKCLGG-LLSKTSIAEWNGVID-KLWEHEEGED 420
E + PNL ++ + I +KC GVPLA+K LG L SKT EW V D ++WE ++ +
Sbjct: 397 EGRNKDYPNLFEMGKDIVRKCGGVPLAVKTLGSQLYSKTDEREWKLVRDSEIWELKQEDA 456
Query: 421 GNKSILPVLRLCYDRMPSHLQRCFLYCSQLTKDRI-LSSNDVIQLWIASDLLPK---ENY 480
G+ ILP LRL Y R+P HL++C CS L KD I SS +I+ W+A +L +
Sbjct: 457 GH--ILPALRLSYTRLPHHLRQCLACCSHLQKDMIEFSSTYLIRYWMAHGILDQSRDHRN 516
Query: 481 LSLEKIGENYFKELCSRCFLQELEEYGLGYWFKLHPLIEKLARILTQKQVFPV--ANTKC 540
+ LE IGE YFK+L R F Q + + G+ Y F +H LI L + + Q + F V ANTK
Sbjct: 517 MELEDIGELYFKDLWVRSFFQNVIDNGMFYRFDMHDLIHDLVQSVAQGECFTVKSANTKD 576
Query: 541 IA---------------------------FTVRDKVPPSAFLANTCIDKFKYIRLLYLGN 600
++ TV++ +F+ TC +FKY+R+L L
Sbjct: 577 MSENVRHLTFLEAGQNVSTTLQKLNKVRTITVQETEIDESFVC-TCFSRFKYLRVLELST 636
Query: 601 ANLREIPNAVEKLVQLRYLDLQGNKRLKRLPNSIFNLKNLQTLILASCSALEELPNHIKQ 660
+L+ +P+++ L LRY++L N+ + +LPN+I L++LQTL L +C L+ELP I +
Sbjct: 637 CSLQVLPSSIGSLKHLRYMNLNSNEAITKLPNAICRLQSLQTLNLCNCENLQELPRDISK 696
Query: 661 LINLRYLWVTANNLRLHKNGVGTMTSLRFLAIGGCENLQDLFEQPSCLVRLETLMIYDSK 720
LI+L LW+T +NGVG + SLRFL+I C NL
Sbjct: 697 LISLTSLWITTKQTSFTENGVGCLKSLRFLSIPSCCNLTS-------------------- 756
Query: 721 TLKLLPNEIGALISLKNLVIWSCKQLTL---TLKGVEFRLRRFTIRELPRVRKLPEWIQR 759
LP E L SL+ L+I +C+QL L +G RL++ I LPR+ LPEW Q
Sbjct: 757 ----LPREKSYLTSLRTLLIRNCEQLDLGNVNYQGTPLRLQKLGIINLPRMVALPEWFQG 788
BLAST of MELO3C009693 vs. TrEMBL
Match:
B9I533_POPTR (NBS-LRR resistance gene-like protein ARGH30 OS=Populus trichocarpa GN=POPTR_0012s01300g PE=3 SV=1)
HSP 1 Score: 340.5 bits (872), Expect = 5.4e-90
Identity = 269/823 (32.69%), Postives = 407/823 (49.45%), Query Frame = 1
Query: 1 MQYTMLSLKTILMDAE-KEEYSHHLNDWLQELQNVFSQIEGLLYEFNGKVKKQEATGKWV 60
++++ML +K +L+DAE K S L WL++L +VF E +L E + +++ +
Sbjct: 37 LKHSMLVIKDVLIDAEEKRSNSPELRLWLKQLNHVFYDAEDVLDELEVENLRRQVIDRGN 96
Query: 61 F-----VLSFNSS-----RIEQTKKMMKLCDDLDEIASQMYDFNLT---------NMET- 120
F + F+SS R +K+ ++ + LD IA+ LT N E
Sbjct: 97 FYTRKVLRCFSSSNPLIFRSTIGRKLKRINEGLDAIAAGNVKCRLTERAEERRPLNRERG 156
Query: 121 THSFLSATEVSTRLMKPSWQLLYPLTNAPKVFHDKRYSNFLDHFKKSTLGFFHIVGEPGI 180
THSF+ + + R + K +K L + + IVG G+
Sbjct: 157 THSFVHSAGIIGR-------------DEDK---EKIIQLLLHPSDEENISVLPIVGIGGM 216
Query: 181 GKTTLAKFFYNDPEVVKTFPSRLWICVKEEFDPRRLIKEMLDFSHCQAT------YDN-- 240
GKTTLAK YND VVK F ++W+ V + D +RL+++++ AT DN
Sbjct: 217 GKTTLAKMAYNDERVVKHFQFKMWVYVSRDSDKKRLMEKLI----ISATGGVGIGEDNGS 276
Query: 241 LTEKQLCFAVQQFLRDKTFLLVFQDISIKNLGDCSIFKSLLGMGNPGSKIIVTTQNEEIA 300
+ ++L +++ +RDK + LV D+ NL K LL +G GS I+VTT++ ++A
Sbjct: 277 MEVEELQTLLRESIRDKKYFLVLDDLWNDNLARWEELKDLLRVGARGSMIMVTTRSNQVA 336
Query: 301 EAIRLGKLCKNESQEVPSPEATEPSDVNNDEAYKDNMTYQKIFEVERLSKENSLSLFKVH 360
I T P V+N ++ + + LSLF +
Sbjct: 337 SMI-----------------GTAPKYVHN---------------LQGVRYDECLSLFVKY 396
Query: 361 AFTETQEAQIPNLTKIQEVIEQKCHGVPLAIKCLGG-LLSKTSIAEWNGVID-KLWEHEE 420
AF E Q+ Q PNL +I E I +KC VPLA++ L G L T +WN + D +LW+ E+
Sbjct: 397 AFKEGQDKQYPNLLRIGEEIVKKCGEVPLAVRTLAGQLFLNTDERDWNLIRDSRLWKVEQ 456
Query: 421 GEDGNKSILPVLRLCYDRMPSHLQRCFLYCSQLTKDRILSSNDVIQLWIASDLLPKENYL 480
ED ILP LR+ Y+++PS L+RCF YCS K+ + ++IQ W+A LL +
Sbjct: 457 KED---DILPALRVSYEQLPSCLKRCFAYCSLFPKNYEYNDYELIQFWMAHGLLQSSDGE 516
Query: 481 S-LEKIGENYFKELCSRCFLQELEEYGLGYWFKLHPLIEKLARILTQKQVFPV-ANTKCI 540
S LE IG Y KEL CFLQ+ + F + ++ LA + Q + F V AN+K I
Sbjct: 517 SELEDIGSIYLKELEYGCFLQDFRDLYGSLQFGMLDVMHDLALSVAQDECFVVTANSKRI 576
Query: 541 AFTVR-------------------------------DK-VPPSAFLANTCIDKFKYIRLL 600
+V+ DK V S + TC+ +FKY+R L
Sbjct: 577 EKSVQHISIPDPDSVRQDFPMLSKELDQVRTVFIHSDKDVLASNSILETCLSRFKYLRAL 636
Query: 601 YLGNANLREIPNAVEKLVQLRYLDLQGNKRLKRLPNSIFNLKNLQTLILASCSALEELPN 660
L + +E+P + KL LRYLDL N R+KRLPNSI L+NLQTL L C +EELP
Sbjct: 637 NLSRSQFKELPKKIGKLKHLRYLDLSWNHRIKRLPNSICKLQNLQTLFLGGCDEIEELPR 696
Query: 661 HIKQLINLRYLWVTANNLRLHKNGVGTMTSLRFLAIGGCENLQDLFEQPSCLVRLETLMI 720
++ + +LR+LW+ L ++ +G + SLRFL I CENL+ LFE L L +L I
Sbjct: 697 GMRYMESLRFLWLATRQTSLPRDEIGCLKSLRFLWIATCENLERLFEDMENLSALRSLYI 756
Query: 721 YDSKTLKLLPNEIGALISLKNLVIWSCKQLTL-TLKGVEFRLRRFTIRELPRVRKLPEWI 756
+L LP I L SL++L I C L + EF+L++ + L V +LPEW+
Sbjct: 757 VTCPSLNSLPPSIKYLTSLQDLHISGCVALNFPNQEACEFKLKKLVLCFLEAVEELPEWL 804
BLAST of MELO3C009693 vs. TAIR10
Match:
AT3G14470.1 (AT3G14470.1 NB-ARC domain-containing disease resistance protein)
HSP 1 Score: 158.7 bits (400), Expect = 1.5e-38
Identity = 173/708 (24.44%), Postives = 311/708 (43.93%), Query Frame = 1
Query: 5 MLSLKTILMDAEKEEYSHHLND-WLQELQNVFSQIEGLLYEFNGKVKKQEATGKWVFVLS 64
+L++ +L+DAE+++ ++ + + W+ EL++V E L + EA + S
Sbjct: 46 LLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDI-----ATEALRLNIGAES 105
Query: 65 FNSSRIEQTKKMMKLCDDLD------EIASQMYDFNLTNMETTHSFLSATEVSTRLMKPS 124
+S+R+ Q + M L D LD E + L + + + L E++ + K
Sbjct: 106 SSSNRLRQLRGRMSLGDFLDGNSEHLETRLEKVTIRLERLASQRNILGLKELTAMIPKQR 165
Query: 125 WQLLYPLTNAPKVF---HDK-RYSNFL--DHFKKSTLGFFHIVGEPGIGKTTLAKFFYND 184
L + +VF DK FL ++ K + + IVG G+GKTTL++ YND
Sbjct: 166 LPTT-SLVDESEVFGRDDDKDEIMRFLIPENGKDNGITVVAIVGIGGVGKTTLSQLLYND 225
Query: 185 PEVVKTFPSRLWICVKEEFDPRRLIKEMLDFSHCQATYDNLTEKQLCFAVQQFLRDK--- 244
V F +++W V EEFD ++ K++ Y+++T + F L+ K
Sbjct: 226 QHVRSYFGTKVWAHVSEEFDVFKITKKV---------YESVTSRPCEFTDLDVLQVKLKE 285
Query: 245 -------TFLLVFQDISIKNLGDCSIFKSLLGMGNPGSKIIVTTQNEEIAEAIRLGKLCK 304
FLLV D+ +N D + + GS+I+VTT+++ +A +C
Sbjct: 286 RLTGTGLPFLLVLDDLWNENFADWDLLRQPFIHAAQGSQILVTTRSQRVASI-----MC- 345
Query: 305 NESQEVPSPEATEPSDVNNDEAYKDNMTYQKIFEVERLSKENSLSLFKVHAFTETQEAQI 364
V+N + D + + ++E L+
Sbjct: 346 -------------AVHVHNLQPLSDGDCWSLFMKTVFGNQEPCLN--------------- 405
Query: 365 PNLTKIQEVIEQKCHGVPLAIKCLGGLLS-KTSIAEWNGVI-DKLWEHEEGEDGNKSILP 424
+ + E I KC G+PLA+K LGG+L + + EW V+ ++W+ + ++LP
Sbjct: 406 REIGDLAERIVHKCRGLPLAVKTLGGVLRFEGKVIEWERVLSSRIWDLPADK---SNLLP 465
Query: 425 VLRLCYDRMPSHLQRCFLYCSQLTKDRILSSNDVIQLWIASDLLPK-ENYLSLEKIGENY 484
VLR+ Y +P+HL+RCF YCS K + V+ LW+A L + + +LE++G Y
Sbjct: 466 VLRVSYYYLPAHLKRCFAYCSIFPKGHAFEKDKVVLLWMAEGFLQQTRSSKNLEELGNEY 525
Query: 485 FKELCSRCFLQELEEYGLGYWFKLHPLIEKLARILTQK---------QVFPVANTKCIAF 544
F EL SR LQ+ + + +H I +LA+ + + ++ T+ +++
Sbjct: 526 FSELESRSLLQKTKTR-----YIMHDFINELAQFASGEFSSKFEDGCKLQVSERTRYLSY 585
Query: 545 TVRDKVPPSAFLANTCIDKFKYIRLLYLGNANLREIPNAVEKLVQLRYLDLQGNKRLKRL 604
+ P F A + + K++R +P ++ + LD +++L L
Sbjct: 586 LRDNYAEPMEFEA---LREVKFLRTF---------LPLSLTNSSRSCCLDQMVSEKL--L 645
Query: 605 PNSIFNLKNLQTLILASCSALEELPNHIKQLINLRYLWVTANNLRLHKNGVGTMTSLRFL 664
P L L+ L L+ P+ K + + R+L ++ L + M +L+ L
Sbjct: 646 P----TLTRLRVLSLSHYKIARLPPDFFKNISHARFLDLSRTELEKLPKSLCYMYNLQTL 677
Query: 665 AIGGCENLQDLFEQPSCLVRLETLMIYDSKTLKLLPNEIGALISLKNL 678
+ C +L++L S L+ L L + +K L+ +P G L SL+ L
Sbjct: 706 LLSYCSSLKELPTDISNLINLRYLDLIGTK-LRQMPRRFGRLKSLQTL 677
BLAST of MELO3C009693 vs. TAIR10
Match:
AT3G50950.2 (AT3G50950.2 HOPZ-ACTIVATED RESISTANCE 1)
HSP 1 Score: 141.7 bits (356), Expect = 1.9e-33
Identity = 160/698 (22.92%), Postives = 292/698 (41.83%), Query Frame = 1
Query: 1 MQYTMLSLKTILMDAEKEEYSHH-LNDWLQELQNVFSQIEGLLYEFN----GKVKKQEAT 60
+Q + +++ L DAE+++ ++ L + +L+ + + E +L + +Q ++
Sbjct: 34 LQSELKYMQSFLKDAERQKRTNETLRTLVADLRELVYEAEDILVDCQLADGDDGNEQRSS 93
Query: 61 GKWVFVLSFNSSRIEQTKKMMKLCDDLDEIASQMYDFNLTNMETTHSFLSATEVSTRLMK 120
W+ L + +R+ K K +++E +++ + +E F++ + V
Sbjct: 94 NAWLSRL--HPARVPLQYKKSKRLQEINERITKIK----SQVEPYFEFITPSNVGRDNGT 153
Query: 121 PSWQL-LYPLTNAPKVFHDKR-YSNFLDHFKKSTLGFFHIVGEPGIGKTTLAKFFYNDPE 180
W +Y T + DKR +L S L VG G+GKTT+A+ +ND E
Sbjct: 154 DRWSSPVYDHTQVVGLEGDKRKIKEWLFRSNDSQLLIMAFVGMGGLGKTTIAQEVFNDKE 213
Query: 181 VVKTFPSRLWICVKEEFDPRRLIKEMLDFSHCQATYDNLTEKQLCFAVQQFLRDKTFLLV 240
+ F R+W+ V + F ++++ +L + D++ L +QQ+L K +L+V
Sbjct: 214 IEHRFERRIWVSVSQTFTEEQIMRSILRNLGDASVGDDIGT--LLRKIQQYLLGKRYLIV 273
Query: 241 FQDISIKNLGDCSIFKSLLGMGNPGSKIIVTTQNEEIAEAIRLGKLCKNESQEVPSPEAT 300
D+ KNL L G GS +IVTT++E +A+ ++
Sbjct: 274 MDDVWDKNLSWWDKIYQGLPRGQGGS-VIVTTRSESVAKRVQA----------------- 333
Query: 301 EPSDVNNDEAYKDNMTYQKIFEVERLSKENSLSLFKVHAFTETQ-EAQIPNLTKIQEVIE 360
+D+ T++ E LS +NS LF AF + P L + + I
Sbjct: 334 -----------RDDKTHRP----ELLSPDNSWLLFCNVAFAANDGTCERPELEDVGKEIV 393
Query: 361 QKCHGVPLAIKCLGGLL-SKTSIA-EWNGVIDKLWEHEEGEDGNK-SILPVLRLCYDRMP 420
KC G+PL IK +GGLL K + EW + + + G +++ L+L YD +P
Sbjct: 394 TKCKGLPLTIKAVGGLLLCKDHVYHEWRRIAEHFQDELRGNTSETDNVMSSLQLSYDELP 453
Query: 421 SHLQRCFLYCSQLTKDRILSSNDVIQLWIASDLLPKENYLSLEKIGENYFKELCSRCFLQ 480
SHL+ C L S +D ++ ++ WI + N S + GE+ F L +RC ++
Sbjct: 454 SHLKSCILTLSLYPEDCVIPKQQLVHGWIGEGFVMWRNGRSATESGEDCFSGLTNRCLIE 513
Query: 481 ELEE--YGLGYWFKLHPLIEKLARILTQKQVFP-VANTKCIAFTVRDKVPPSAFLANTCI 540
+++ G K+H ++ L + +K F C + N +
Sbjct: 514 VVDKTYSGTIITCKIHDMVRDLVIDIAKKDSFSNPEGLNCRHLGISGNFDEKQIKVNHKL 573
Query: 541 DKFKYIRLLYLGNANLREIPNAVEKLVQLRYLDLQG---NKRLKRLPNSIFNLKNLQTLI 600
N ++ LR LD+ + L + + I +L++L L
Sbjct: 574 RGVVSTTKTGEVNKLNSDLAKKFTDCKYLRVLDISKSIFDAPLSEILDEIASLQHLACLS 633
Query: 601 LASCSALEELPNHIKQLINLRYLWVTANNLRLHKNGVGTMTSLRFLAIGGCENLQDLFEQ 660
L++ L + P ++ L N L+ L C+NL+ L Q
Sbjct: 634 LSNTHPLIQFPRSMEDLHN-----------------------LQILDASYCQNLKQL--Q 664
Query: 661 PSCLVRLETLMIYDSK---TLKLLPNEIGALISLKNLV 679
P C+V + L++ D +L+ P IG+L+ L+ L+
Sbjct: 694 P-CIVLFKKLLVLDMTNCGSLECFPKGIGSLVKLEVLL 664
HSP 2 Score: 42.0 bits (97), Expect = 2.0e-03
Identity = 38/133 (28.57%), Postives = 58/133 (43.61%), Query Frame = 1
Query: 526 IDKFKYIRLLYLGNAN-LREIPNAVEKLVQLRYLDLQGNKRLKRLPNSIFNLKNLQTLIL 585
I +++ L L N + L + P ++E L L+ LD + LK+L I K L L +
Sbjct: 582 IASLQHLACLSLSNTHPLIQFPRSMEDLHNLQILDASYCQNLKQLQPCIVLFKKLLVLDM 641
Query: 586 ASCSALEELPNHIKQLINLRYLW---VTANNLRLHKNGVGTMTSLRFLAIGGCENLQDLF 645
+C +LE P I L+ L L +N + V +T+LR L + Q
Sbjct: 642 TNCGSLECFPKGIGSLVKLEVLLGFKPARSNNGCKLSEVKNLTNLRKLGLSLTRGDQIEE 701
Query: 646 EQPSCLVRLETLM 655
E+ L+ L LM
Sbjct: 702 EELDSLINLSKLM 714
BLAST of MELO3C009693 vs. TAIR10
Match:
AT3G14460.1 (AT3G14460.1 LRR and NB-ARC domains-containing disease resistance protein)
HSP 1 Score: 127.9 bits (320), Expect = 2.8e-29
Identity = 109/392 (27.81%), Postives = 173/392 (44.13%), Query Frame = 1
Query: 283 ESQEVPSPEATEPSDV---NNDEAYKDNMTYQKIFEVERLSKENSLSLFKVHAFTETQEA 342
ES +V +A E S + E +KI++++ ++ E L AF
Sbjct: 290 ESFQVAFTDAEEGSKIVLTTRSEIVSTVAKAEKIYQMKLMTNEECWELISRFAFGNISVG 349
Query: 343 QI-PNLTKIQEVIEQKCHGVPLAIKCLGG-LLSKTSIAEWNGVIDKLWEHEEGEDGNKSI 402
I L I + I ++C G+PLA + + L SK + +W V + SI
Sbjct: 350 SINQELEGIGKRIAEQCKGLPLAARAIASHLRSKPNPDDWYAVSKNFSSY------TNSI 409
Query: 403 LPVLRLCYDRMPSHLQRCFLYCSQLTKDRILSSNDVIQLWIASDLLPK-ENYLSLEKIGE 462
LPVL+L YD +P L+RCF CS K + +++ LW+A DLL + + LE IG
Sbjct: 410 LPVLKLSYDSLPPQLKRCFALCSIFPKGHVFDREELVLLWMAIDLLYQPRSSRRLEDIGN 469
Query: 463 NYFKELCSRCFLQELEEYGLGYWFKLHPLIEKLAR-----------------ILTQKQVF 522
+Y +L ++ F Q L+ F +H L+ LA+ I + + F
Sbjct: 470 DYLGDLVAQSFFQRLDITMTS--FVMHDLMNDLAKAVSGDFCFRLEDDNIPEIPSTTRHF 529
Query: 523 PVANTKCIAFTVRDKVPPSAFLA---------------------NTCIDKFKYIRLLYLG 582
+ ++C A + + FL N ++ +R+L L
Sbjct: 530 SFSRSQCDASVAFRSICGAEFLRTILPFNSPTSLESLQLTEKVLNPLLNALSGLRILSLS 589
Query: 583 NANLREIPNAVEKLVQLRYLDLQGNKRLKRLPNSIFNLKNLQTLILASCSALEELPNHIK 631
+ + +P +++ L LRYLDL K +K LP + L NLQTL+L++C L LP I
Sbjct: 590 HYQITNLPKSLKGLKLLRYLDLSSTK-IKELPEFVCTLCNLQTLLLSNCRDLTSLPKSIA 649
HSP 2 Score: 103.6 bits (257), Expect = 5.7e-22
Identity = 108/414 (26.09%), Postives = 185/414 (44.69%), Query Frame = 1
Query: 283 ESQEVPSPEATEPSDV---NNDEAYKDNMTYQKIFEVERLSKENSLSLFKVHAFTETQEA 342
ES +V +A E S + E +KI++++ ++ E L AF
Sbjct: 290 ESFQVAFTDAEEGSKIVLTTRSEIVSTVAKAEKIYQMKLMTNEECWELISRFAFGNISVG 349
Query: 343 QI-PNLTKIQEVIEQKCHGVPLAIKCLGG-LLSKTSIAEWNGVIDKLWEHEEGEDGNKSI 402
I L I + I ++C G+PLA + + L SK + +W V + SI
Sbjct: 350 SINQELEGIGKRIAEQCKGLPLAARAIASHLRSKPNPDDWYAVSKNFSSY------TNSI 409
Query: 403 LPVLRLCYDRMPSHLQRCFLYCSQLTKDRILSSNDVIQLWIASDLL-PKENYLSLEKIGE 462
LPVL+L YD +P L+RCF CS K + +++ LW+A DLL + LE IG
Sbjct: 410 LPVLKLSYDSLPPQLKRCFALCSIFPKGHVFDREELVLLWMAIDLLYQPRSSRRLEDIGN 469
Query: 463 NYFKELCSRCFLQELEEYGLGYWFKLHPLIEKLARILTQKQVFPV---------ANTKCI 522
+Y +L ++ F Q L+ F +H L+ LA+ ++ F + + T+
Sbjct: 470 DYLGDLVAQSFFQRLDITMTS--FVMHDLMNDLAKAVSGDFCFRLEDDNIPEIPSTTRHF 529
Query: 523 AFTVRDKVPPSAFLANTCIDKFKYIRLLYLGNANLREIPNAVEKLVQLRYLDLQGNKRLK 582
+F+ R + S + C +F +R + N+ P ++E L QL K L
Sbjct: 530 SFS-RSQCDASVAFRSICGAEF--LRTILPFNS-----PTSLESL-QLT------EKVLN 589
Query: 583 RLPNSIFNLKNLQTLILASCSALEELPNHIKQLINLRYLWVTANNLRLHKNGVGTMTSLR 642
L N++ L+ ++ S + LP +K L LRYL +++ ++ V T+ +L+
Sbjct: 590 PLLNALSGLR----ILSLSHYQITNLPKSLKGLKLLRYLDLSSTKIKELPEFVCTLCNLQ 649
Query: 643 FLAIGGCENLQDLFEQPSCLVRLETLMIYDSKTLKLLP--NEIGALISLKNLVI 680
L + C +L L + + L+ L L + + +++ P ++ +L L N VI
Sbjct: 650 TLLLSNCRDLTSLPKSIAELINLRLLDLVGTPLVEMPPGIKKLRSLQKLSNFVI 676
HSP 3 Score: 61.2 bits (147), Expect = 3.2e-09
Identity = 43/137 (31.39%), Postives = 67/137 (48.91%), Query Frame = 1
Query: 153 IVGEPGIGKTTLAKFFYNDPEVVKTFPSRLWICVKEEFDPRRLIKEML-DFSHCQATYDN 212
+VG PG+GKTTL + +ND V + F ++WI F+ + K +L D + ++
Sbjct: 198 VVGMPGVGKTTLTEIVFNDYRVTEHFEVKMWISAGINFNVFTVTKAVLQDITSSAVNTED 257
Query: 213 LTEKQLCFAVQQFLRDKTFLLVFQDISIKNLGDCSIFKSLLGMGNPGSKIIVTTQNEEI- 272
L Q+ +++ L K FLLV D ++ + F+ GSKI++TT++E +
Sbjct: 258 LPSLQI--QLKKTLSGKRFLLVLDDFWSESDSEWESFQVAFTDAEEGSKIVLTTRSEIVS 317
Query: 273 ----AEAIRLGKLCKNE 284
AE I KL NE
Sbjct: 318 TVAKAEKIYQMKLMTNE 332
BLAST of MELO3C009693 vs. TAIR10
Match:
AT4G27220.1 (AT4G27220.1 NB-ARC domain-containing disease resistance protein)
HSP 1 Score: 114.4 bits (285), Expect = 3.2e-25
Identity = 92/374 (24.60%), Postives = 176/374 (47.06%), Query Frame = 1
Query: 302 EAYKDNMTYQKIFEVERLSKENSLSLFKVHAFTETQEAQIPNLTKIQEVIEQKCHGVPLA 361
E + MT + I +V L ++ + LF H E A N+ I + + +C G+PLA
Sbjct: 256 EVCQQMMTNENI-KVACLQEKEAWELF-CHNVGEV--ANSDNVKPIAKDVSHECCGLPLA 315
Query: 362 IKCLGGLL-SKTSIAEWNGVIDKLWEHEEGEDGNKSILPVLRLCYDRMPSHLQRCFLYCS 421
I +G L K + W ++ L D + I L+L YD + +++ CFL+C+
Sbjct: 316 IITIGRTLRGKPQVEVWKHTLNLLKRSAPSIDTEEKIFGTLKLSYDFLQDNMKSCFLFCA 375
Query: 422 QLTKDRILSSNDVIQLWIASDLLP-KENYLSLEKIGENYFKELCSRCFLQELEEYGLGYW 481
+D + +++I W+A LL + +Y + G + L C L++ +
Sbjct: 376 LFPEDYSIKVSELIMYWVAEGLLDGQHHYEDMMNEGVTLVERLKDSCLLEDGDSCDT--- 435
Query: 482 FKLHPLIEKLA-------------RILTQKQVFPVANTKCIAFTVRDKVPPSAF--LANT 541
K+H ++ A ++ + + K ++ R + + L N
Sbjct: 436 VKMHDVVRDFAIWFMSSQGEGFHSLVMAGRGLIEFPQDKFVSSVQRVSLMANKLERLPNN 495
Query: 542 CIDKFKYIRLLYLGNANLREIPNA-VEKLVQLRYLDLQGNKRLKRLPNSIFNLKNLQTLI 601
I+ + + LL GN++++E+PN ++ LR LDL G R++ LP+S NL +L++L+
Sbjct: 496 VIEGVETLVLLLQGNSHVKEVPNGFLQAFPNLRILDLSG-VRIRTLPDSFSNLHSLRSLV 555
Query: 602 LASCSALEELPNHIKQLINLRYLWVTANNLRLHKNGVGTMTSLRFLAIGGCENLQDLFEQ 658
L +C L LP+ ++ L+ L++L + + +R G+ ++SLR++ + LQ +
Sbjct: 556 LRNCKKLRNLPS-LESLVKLQFLDLHESAIRELPRGLEALSSLRYICVSNTYQLQSI--P 615
BLAST of MELO3C009693 vs. TAIR10
Match:
AT1G59780.1 (AT1G59780.1 NB-ARC domain-containing disease resistance protein)
HSP 1 Score: 108.2 bits (269), Expect = 2.3e-23
Identity = 124/535 (23.18%), Postives = 234/535 (43.74%), Query Frame = 1
Query: 153 IVGEPGIGKTTLAKFFYNDPEVVKTFPSRLWICVKEEFDPRRLIKEMLDFSHCQATYDNL 212
I G G+GKTTLA+ ++ +V F W+CV +EF + + K +L + +L
Sbjct: 185 ITGLGGLGKTTLARQIFDHDKVKSHFDGLAWVCVSQEFTRKDVWKTILGNLSPKYKDSDL 244
Query: 213 TEKQLCFAVQQFLRDKTFLLVFQDISIKNLGDCSIFKSLLGMGNPGSKIIVTTQNEEIAE 272
E + + Q L K L+VF D+ + D + G K+++T++N+ I
Sbjct: 245 PEDDIQKKLFQLLETKKALIVFDDLWKRE--DWYRIAPMFPERKAGWKVLLTSRNDAIH- 304
Query: 273 AIRLGKLCKNESQEVPSPEATEPSDVNNDEAYKDNMTYQKIFEVERLSKENSLSLFKVHA 332
P +P + +DE + K+ + SK+ +++ + +
Sbjct: 305 ---------------PHCVTFKPELLTHDECW-------KLLQRIAFSKQKTITGYIID- 364
Query: 333 FTETQEAQIPNLTKIQEVIEQKCHGVPLAIKCLGGLL-SKTSIAEWNGVIDKLWEH---- 392
+ K+ + + + C +PLA+K LGGLL +K ++ +W + + + H
Sbjct: 365 ---------KEMVKMAKEMTKHCKRLPLAVKLLGGLLDAKHTLRQWKLISENIISHIVVG 424
Query: 393 --EEGEDGNKSILPVLRLCYDRMPSHLQRCFLYCSQLTKDRILSSNDVIQLWIASDLLPK 452
E+ + S+ VL L ++ +P +L+ C LY + +D + + +W A +
Sbjct: 425 GTSSNENDSSSVNHVLSLSFEGLPGYLKHCLLYLASYPEDHEIEIERLSYVWAAEGITYP 484
Query: 453 ENY--LSLEKIGENYFKELCSRCFLQELEEYGLGYWF---KLHPLIEKLARILTQK---- 512
NY ++ + + Y +EL R + E L F +LH L+ ++ + ++
Sbjct: 485 GNYEGATIRDVADLYIEELVKRNMVIS-ERDALTSRFEKCQLHDLMREICLLKAKEENFL 544
Query: 513 QVFPVANTKCIAFTVRDKVPPSAFLANTCI---------DKFKYIRLLYLGNANLREIPN 572
Q+ + ++ + NT I K + + + +G + N
Sbjct: 545 QIVTDPTSSSSVHSLASSRSRRLVVYNTSIFSGENDMKNSKLRSLLFIPVGYSRFSMGSN 604
Query: 573 AVEKLVQLRYLDLQGNK-RLKRLPNSIFNLKNLQTLILASCSALEELPNHIKQLINLRYL 632
+E L LR LDL G K + +LP+SI L +L+ L L S + LP+ ++ L +L YL
Sbjct: 605 FIE-LPLLRVLDLDGAKFKGGKLPSSIGKLIHLKYLSLYQAS-VTYLPSSLRNLKSLLYL 664
Query: 633 WVTANNLRLHK--NGVGTMTSLRFLAIGGCENLQDLFEQPSCLVRLETLMIYDSK 660
+ N+ +L N M LR+L++ + E + L++LETL+ + +K
Sbjct: 665 NLRINSGQLINVPNVFKEMLELRYLSLPWERSSLTKLELGN-LLKLETLINFSTK 680
HSP 2 Score: 51.2 bits (121), Expect = 3.3e-06
Identity = 57/223 (25.56%), Postives = 105/223 (47.09%), Query Frame = 1
Query: 532 IRLLYLGNANLR--EIPNAVEKLVQLRYLDLQGNKRLKRLPNSIFNLKNLQTLILASCSA 591
+R+L L A + ++P+++ KL+ L+YL L + LP+S+ NLK+L L L S
Sbjct: 575 LRVLDLDGAKFKGGKLPSSIGKLIHLKYLSLY-QASVTYLPSSLRNLKSLLYLNLRINSG 634
Query: 592 -LEELPNHIKQLINLRYLWVTANNLRLHKNGVGTMTSLRFLAIGGCE--NLQDLFEQPSC 651
L +PN K+++ LRYL + L K +G + L L + ++ DL
Sbjct: 635 QLINVPNVFKEMLELRYLSLPWERSSLTKLELGNLLKLETLINFSTKDSSVTDLHRMTK- 694
Query: 652 LVRLETLMIYDSKTLKLLPNEIGALISLKNLVIWSCKQLTLTLKGVEFRLRRFTIRELPR 711
L L+ L+ + ++ L + + L L++L + T + V+F+ + R +
Sbjct: 695 LRTLQILISGEGLHMETLSSALSMLGHLEDLTV------TPSENSVQFKHPKLIYRPM-- 754
Query: 712 VRKLPEWIQRFTESLRVLEIIDCPIELKDDEFKSYKSLERLSI 750
LP+ +Q F L + ++ C +E +D + + L +L +
Sbjct: 755 ---LPD-VQHFPSHLTTISLVYCFLE--EDPMPTLEKLLQLKV 781
HSP 3 Score: 37.4 bits (85), Expect = 5.0e-02
Identity = 31/104 (29.81%), Postives = 46/104 (44.23%), Query Frame = 1
Query: 578 NLQTLILASCSALEELPNHIKQLINLRY--LWVTANNLRLHKNGVGTMTSLRFLAIGGCE 637
+L T+ L C E+ +++L+ L+ LW A R G L L I G +
Sbjct: 754 HLTTISLVYCFLEEDPMPTLEKLLQLKVVSLWYNAYVGRRMVCTGGGFPPLHRLEIWGLD 813
Query: 638 NLQDLFEQPSCLVRLETLMIYDSKTLKLLPNEIGALISLKNLVI 680
L++ + + L TL I D K LK +P+ + + SLK L I
Sbjct: 814 ALEEWIVEEGSMPLLHTLHIVDCKKLKEIPDGLRFISSLKELAI 857
BLAST of MELO3C009693 vs. NCBI nr
Match:
gi|659084959|ref|XP_008443166.1| (PREDICTED: putative disease resistance protein RGA4 [Cucumis melo])
HSP 1 Score: 1585.5 bits (4104), Expect = 0.0e+00
Identity = 785/785 (100.00%), Postives = 785/785 (100.00%), Query Frame = 1
Query: 1 MQYTMLSLKTILMDAEKEEYSHHLNDWLQELQNVFSQIEGLLYEFNGKVKKQEATGKWVF 60
MQYTMLSLKTILMDAEKEEYSHHLNDWLQELQNVFSQIEGLLYEFNGKVKKQEATGKWVF
Sbjct: 26 MQYTMLSLKTILMDAEKEEYSHHLNDWLQELQNVFSQIEGLLYEFNGKVKKQEATGKWVF 85
Query: 61 VLSFNSSRIEQTKKMMKLCDDLDEIASQMYDFNLTNMETTHSFLSATEVSTRLMKPSWQL 120
VLSFNSSRIEQTKKMMKLCDDLDEIASQMYDFNLTNMETTHSFLSATEVSTRLMKPSWQL
Sbjct: 86 VLSFNSSRIEQTKKMMKLCDDLDEIASQMYDFNLTNMETTHSFLSATEVSTRLMKPSWQL 145
Query: 121 LYPLTNAPKVFHDKRYSNFLDHFKKSTLGFFHIVGEPGIGKTTLAKFFYNDPEVVKTFPS 180
LYPLTNAPKVFHDKRYSNFLDHFKKSTLGFFHIVGEPGIGKTTLAKFFYNDPEVVKTFPS
Sbjct: 146 LYPLTNAPKVFHDKRYSNFLDHFKKSTLGFFHIVGEPGIGKTTLAKFFYNDPEVVKTFPS 205
Query: 181 RLWICVKEEFDPRRLIKEMLDFSHCQATYDNLTEKQLCFAVQQFLRDKTFLLVFQDISIK 240
RLWICVKEEFDPRRLIKEMLDFSHCQATYDNLTEKQLCFAVQQFLRDKTFLLVFQDISIK
Sbjct: 206 RLWICVKEEFDPRRLIKEMLDFSHCQATYDNLTEKQLCFAVQQFLRDKTFLLVFQDISIK 265
Query: 241 NLGDCSIFKSLLGMGNPGSKIIVTTQNEEIAEAIRLGKLCKNESQEVPSPEATEPSDVNN 300
NLGDCSIFKSLLGMGNPGSKIIVTTQNEEIAEAIRLGKLCKNESQEVPSPEATEPSDVNN
Sbjct: 266 NLGDCSIFKSLLGMGNPGSKIIVTTQNEEIAEAIRLGKLCKNESQEVPSPEATEPSDVNN 325
Query: 301 DEAYKDNMTYQKIFEVERLSKENSLSLFKVHAFTETQEAQIPNLTKIQEVIEQKCHGVPL 360
DEAYKDNMTYQKIFEVERLSKENSLSLFKVHAFTETQEAQIPNLTKIQEVIEQKCHGVPL
Sbjct: 326 DEAYKDNMTYQKIFEVERLSKENSLSLFKVHAFTETQEAQIPNLTKIQEVIEQKCHGVPL 385
Query: 361 AIKCLGGLLSKTSIAEWNGVIDKLWEHEEGEDGNKSILPVLRLCYDRMPSHLQRCFLYCS 420
AIKCLGGLLSKTSIAEWNGVIDKLWEHEEGEDGNKSILPVLRLCYDRMPSHLQRCFLYCS
Sbjct: 386 AIKCLGGLLSKTSIAEWNGVIDKLWEHEEGEDGNKSILPVLRLCYDRMPSHLQRCFLYCS 445
Query: 421 QLTKDRILSSNDVIQLWIASDLLPKENYLSLEKIGENYFKELCSRCFLQELEEYGLGYWF 480
QLTKDRILSSNDVIQLWIASDLLPKENYLSLEKIGENYFKELCSRCFLQELEEYGLGYWF
Sbjct: 446 QLTKDRILSSNDVIQLWIASDLLPKENYLSLEKIGENYFKELCSRCFLQELEEYGLGYWF 505
Query: 481 KLHPLIEKLARILTQKQVFPVANTKCIAFTVRDKVPPSAFLANTCIDKFKYIRLLYLGNA 540
KLHPLIEKLARILTQKQVFPVANTKCIAFTVRDKVPPSAFLANTCIDKFKYIRLLYLGNA
Sbjct: 506 KLHPLIEKLARILTQKQVFPVANTKCIAFTVRDKVPPSAFLANTCIDKFKYIRLLYLGNA 565
Query: 541 NLREIPNAVEKLVQLRYLDLQGNKRLKRLPNSIFNLKNLQTLILASCSALEELPNHIKQL 600
NLREIPNAVEKLVQLRYLDLQGNKRLKRLPNSIFNLKNLQTLILASCSALEELPNHIKQL
Sbjct: 566 NLREIPNAVEKLVQLRYLDLQGNKRLKRLPNSIFNLKNLQTLILASCSALEELPNHIKQL 625
Query: 601 INLRYLWVTANNLRLHKNGVGTMTSLRFLAIGGCENLQDLFEQPSCLVRLETLMIYDSKT 660
INLRYLWVTANNLRLHKNGVGTMTSLRFLAIGGCENLQDLFEQPSCLVRLETLMIYDSKT
Sbjct: 626 INLRYLWVTANNLRLHKNGVGTMTSLRFLAIGGCENLQDLFEQPSCLVRLETLMIYDSKT 685
Query: 661 LKLLPNEIGALISLKNLVIWSCKQLTLTLKGVEFRLRRFTIRELPRVRKLPEWIQRFTES 720
LKLLPNEIGALISLKNLVIWSCKQLTLTLKGVEFRLRRFTIRELPRVRKLPEWIQRFTES
Sbjct: 686 LKLLPNEIGALISLKNLVIWSCKQLTLTLKGVEFRLRRFTIRELPRVRKLPEWIQRFTES 745
Query: 721 LRVLEIIDCPIELKDDEFKSYKSLERLSIHGAVRIKNLNGEYCIDFQNSVRRREVTKEMK 780
LRVLEIIDCPIELKDDEFKSYKSLERLSIHGAVRIKNLNGEYCIDFQNSVRRREVTKEMK
Sbjct: 746 LRVLEIIDCPIELKDDEFKSYKSLERLSIHGAVRIKNLNGEYCIDFQNSVRRREVTKEMK 805
Query: 781 TCYYY 786
TCYYY
Sbjct: 806 TCYYY 810
BLAST of MELO3C009693 vs. NCBI nr
Match:
gi|778688885|ref|XP_004136756.2| (PREDICTED: putative disease resistance protein RGA4 [Cucumis sativus])
HSP 1 Score: 1349.3 bits (3491), Expect = 0.0e+00
Identity = 677/785 (86.24%), Postives = 718/785 (91.46%), Query Frame = 1
Query: 1 MQYTMLSLKTILMDAEKEEYSHHLNDWLQELQNVFSQIEGLLYEFNGKVKKQEATGKWVF 60
MQYTMLSLKTIL DAEKEEY H LNDWLQ+LQ+VF QIE LLYE N +VKKQEATGKWVF
Sbjct: 57 MQYTMLSLKTILKDAEKEEYRHCLNDWLQKLQSVFLQIEELLYESNREVKKQEATGKWVF 116
Query: 61 VLSFNSSRIEQTKKMMKLCDDLDEIASQMYDFNLTNMETTHSFLSATEVSTRLMKPSWQL 120
+ SFN S+I+QTKKMMKLCDDLDEIAS MY FNLTNMETTHSFLSATEVSTRLMKPSWQL
Sbjct: 117 LPSFNFSQIDQTKKMMKLCDDLDEIASHMYGFNLTNMETTHSFLSATEVSTRLMKPSWQL 176
Query: 121 LYPLTNAPKVFHDKRYSNFLDHFKKSTLGFFHIVGEPGIGKTTLAKFFYNDPEVVKTFPS 180
LY LTNAPKVF DKRY NFLDHFKKST G FHIVGEPGIGKTTLAKFFYN+ +V TFPS
Sbjct: 177 LYSLTNAPKVFQDKRYHNFLDHFKKSTHGLFHIVGEPGIGKTTLAKFFYNN--LVNTFPS 236
Query: 181 RLWICVKEEFDPRRLIKEMLDFSHCQATYDNLTEKQLCFAVQQFLRDKTFLLVFQDISIK 240
RLWICVKEEFDP+RLIKEML FSHCQ T DNLTEKQLCFAVQQFLRDK FL+VFQDISIK
Sbjct: 237 RLWICVKEEFDPQRLIKEMLSFSHCQVTCDNLTEKQLCFAVQQFLRDKKFLIVFQDISIK 296
Query: 241 NLGDCSIFKSLLGMGNPGSKIIVTTQNEEIAEAIRLGKLCKNESQEVPSPEATEPSDVNN 300
NLG+CSIFKSLLGMGN GSKIIVTTQNE+IA+A+ L KL KNESQ VPSPEAT+PSDVN
Sbjct: 297 NLGNCSIFKSLLGMGNRGSKIIVTTQNEKIADAVGLKKLYKNESQVVPSPEATKPSDVN- 356
Query: 301 DEAYKDNMTYQKIFEVERLSKENSLSLFKVHAFTETQEAQIPNLTKIQEVIEQKCHGVPL 360
KDNM +Q IF+VERLSKENSLSLFKVHAFTETQEAQIPNLTKIQEVIEQKCHGVPL
Sbjct: 357 ----KDNMKHQTIFKVERLSKENSLSLFKVHAFTETQEAQIPNLTKIQEVIEQKCHGVPL 416
Query: 361 AIKCLGGLLSKTSIAEWNGVIDKLWEHEEGEDGNKSILPVLRLCYDRMPSHLQRCFLYCS 420
AIKCLGGLLSKTSIAEWNGVIDKLWEHEE EDGNKSILP LRLCYD+MPSHLQRCFLYCS
Sbjct: 417 AIKCLGGLLSKTSIAEWNGVIDKLWEHEEEEDGNKSILPTLRLCYDQMPSHLQRCFLYCS 476
Query: 421 QLTKDRILSSNDVIQLWIASDLLPKENYLSLEKIGENYFKELCSRCFLQELEEYGLGYWF 480
QL KDRILSSNDVIQLWIASDLLPKENYLSLEKIGENYFKELCSRCFLQELEEYG GYWF
Sbjct: 477 QLKKDRILSSNDVIQLWIASDLLPKENYLSLEKIGENYFKELCSRCFLQELEEYGFGYWF 536
Query: 481 KLHPLIEKLARILTQKQVFPVANTKCIAFTVRDKVPPSAFLANTCIDKFKYIRLLYLGNA 540
KLHPLIEKLAR+LTQKQVF V T+ IAFT+RDKVPPSAFLAN CIDKFKY+RLL+LGNA
Sbjct: 537 KLHPLIEKLARLLTQKQVFEVTKTQSIAFTIRDKVPPSAFLANACIDKFKYLRLLHLGNA 596
Query: 541 NLREIPNAVEKLVQLRYLDLQGNKRLKRLPNSIFNLKNLQTLILASCSALEELPNHIKQL 600
NL+ IP+AVE LVQLRYLDLQGNK++KRLPNSIF LKNLQTLILASCSAL+ELPN I+QL
Sbjct: 597 NLQGIPSAVENLVQLRYLDLQGNKKIKRLPNSIFKLKNLQTLILASCSALKELPNDIRQL 656
Query: 601 INLRYLWVTANNLRLHKNGVGTMTSLRFLAIGGCENLQDLFEQPSCLVRLETLMIYDSKT 660
NLRYLWVTANNLRLHKNGVGTMTSLRFLAIGGC+NLQDLF++PSCLVRLETLMIYD KT
Sbjct: 657 TNLRYLWVTANNLRLHKNGVGTMTSLRFLAIGGCQNLQDLFKKPSCLVRLETLMIYDCKT 716
Query: 661 LKLLPNEIGALISLKNLVIWSCKQLTLTLKGVEFRLRRFTIRELPRVRKLPEWIQRFTES 720
LK LPNEIG+LISLKNLVIWSCK+LTLTLKGVEFRL+RFTIRELP V+KLPEW QRFTE+
Sbjct: 717 LKSLPNEIGSLISLKNLVIWSCKKLTLTLKGVEFRLQRFTIRELPIVKKLPEWTQRFTET 776
Query: 721 LRVLEIIDCPIELKDDEFKSYKSLERLSIHGAVRIKNLNGEYCIDFQNSVRRREVTKEMK 780
LRVLEIIDCPIE DD KSYKSLER SIHGAVR KN G Y ID++N VR R+V KE+K
Sbjct: 777 LRVLEIIDCPIEWNDDVLKSYKSLERFSIHGAVRTKNQIGGYNIDYRNFVRSRKVKKEVK 834
Query: 781 TCYYY 786
TC YY
Sbjct: 837 TCVYY 834
BLAST of MELO3C009693 vs. NCBI nr
Match:
gi|700204239|gb|KGN59372.1| (hypothetical protein Csa_3G814390 [Cucumis sativus])
HSP 1 Score: 1349.3 bits (3491), Expect = 0.0e+00
Identity = 677/785 (86.24%), Postives = 718/785 (91.46%), Query Frame = 1
Query: 1 MQYTMLSLKTILMDAEKEEYSHHLNDWLQELQNVFSQIEGLLYEFNGKVKKQEATGKWVF 60
MQYTMLSLKTIL DAEKEEY H LNDWLQ+LQ+VF QIE LLYE N +VKKQEATGKWVF
Sbjct: 26 MQYTMLSLKTILKDAEKEEYRHCLNDWLQKLQSVFLQIEELLYESNREVKKQEATGKWVF 85
Query: 61 VLSFNSSRIEQTKKMMKLCDDLDEIASQMYDFNLTNMETTHSFLSATEVSTRLMKPSWQL 120
+ SFN S+I+QTKKMMKLCDDLDEIAS MY FNLTNMETTHSFLSATEVSTRLMKPSWQL
Sbjct: 86 LPSFNFSQIDQTKKMMKLCDDLDEIASHMYGFNLTNMETTHSFLSATEVSTRLMKPSWQL 145
Query: 121 LYPLTNAPKVFHDKRYSNFLDHFKKSTLGFFHIVGEPGIGKTTLAKFFYNDPEVVKTFPS 180
LY LTNAPKVF DKRY NFLDHFKKST G FHIVGEPGIGKTTLAKFFYN+ +V TFPS
Sbjct: 146 LYSLTNAPKVFQDKRYHNFLDHFKKSTHGLFHIVGEPGIGKTTLAKFFYNN--LVNTFPS 205
Query: 181 RLWICVKEEFDPRRLIKEMLDFSHCQATYDNLTEKQLCFAVQQFLRDKTFLLVFQDISIK 240
RLWICVKEEFDP+RLIKEML FSHCQ T DNLTEKQLCFAVQQFLRDK FL+VFQDISIK
Sbjct: 206 RLWICVKEEFDPQRLIKEMLSFSHCQVTCDNLTEKQLCFAVQQFLRDKKFLIVFQDISIK 265
Query: 241 NLGDCSIFKSLLGMGNPGSKIIVTTQNEEIAEAIRLGKLCKNESQEVPSPEATEPSDVNN 300
NLG+CSIFKSLLGMGN GSKIIVTTQNE+IA+A+ L KL KNESQ VPSPEAT+PSDVN
Sbjct: 266 NLGNCSIFKSLLGMGNRGSKIIVTTQNEKIADAVGLKKLYKNESQVVPSPEATKPSDVN- 325
Query: 301 DEAYKDNMTYQKIFEVERLSKENSLSLFKVHAFTETQEAQIPNLTKIQEVIEQKCHGVPL 360
KDNM +Q IF+VERLSKENSLSLFKVHAFTETQEAQIPNLTKIQEVIEQKCHGVPL
Sbjct: 326 ----KDNMKHQTIFKVERLSKENSLSLFKVHAFTETQEAQIPNLTKIQEVIEQKCHGVPL 385
Query: 361 AIKCLGGLLSKTSIAEWNGVIDKLWEHEEGEDGNKSILPVLRLCYDRMPSHLQRCFLYCS 420
AIKCLGGLLSKTSIAEWNGVIDKLWEHEE EDGNKSILP LRLCYD+MPSHLQRCFLYCS
Sbjct: 386 AIKCLGGLLSKTSIAEWNGVIDKLWEHEEEEDGNKSILPTLRLCYDQMPSHLQRCFLYCS 445
Query: 421 QLTKDRILSSNDVIQLWIASDLLPKENYLSLEKIGENYFKELCSRCFLQELEEYGLGYWF 480
QL KDRILSSNDVIQLWIASDLLPKENYLSLEKIGENYFKELCSRCFLQELEEYG GYWF
Sbjct: 446 QLKKDRILSSNDVIQLWIASDLLPKENYLSLEKIGENYFKELCSRCFLQELEEYGFGYWF 505
Query: 481 KLHPLIEKLARILTQKQVFPVANTKCIAFTVRDKVPPSAFLANTCIDKFKYIRLLYLGNA 540
KLHPLIEKLAR+LTQKQVF V T+ IAFT+RDKVPPSAFLAN CIDKFKY+RLL+LGNA
Sbjct: 506 KLHPLIEKLARLLTQKQVFEVTKTQSIAFTIRDKVPPSAFLANACIDKFKYLRLLHLGNA 565
Query: 541 NLREIPNAVEKLVQLRYLDLQGNKRLKRLPNSIFNLKNLQTLILASCSALEELPNHIKQL 600
NL+ IP+AVE LVQLRYLDLQGNK++KRLPNSIF LKNLQTLILASCSAL+ELPN I+QL
Sbjct: 566 NLQGIPSAVENLVQLRYLDLQGNKKIKRLPNSIFKLKNLQTLILASCSALKELPNDIRQL 625
Query: 601 INLRYLWVTANNLRLHKNGVGTMTSLRFLAIGGCENLQDLFEQPSCLVRLETLMIYDSKT 660
NLRYLWVTANNLRLHKNGVGTMTSLRFLAIGGC+NLQDLF++PSCLVRLETLMIYD KT
Sbjct: 626 TNLRYLWVTANNLRLHKNGVGTMTSLRFLAIGGCQNLQDLFKKPSCLVRLETLMIYDCKT 685
Query: 661 LKLLPNEIGALISLKNLVIWSCKQLTLTLKGVEFRLRRFTIRELPRVRKLPEWIQRFTES 720
LK LPNEIG+LISLKNLVIWSCK+LTLTLKGVEFRL+RFTIRELP V+KLPEW QRFTE+
Sbjct: 686 LKSLPNEIGSLISLKNLVIWSCKKLTLTLKGVEFRLQRFTIRELPIVKKLPEWTQRFTET 745
Query: 721 LRVLEIIDCPIELKDDEFKSYKSLERLSIHGAVRIKNLNGEYCIDFQNSVRRREVTKEMK 780
LRVLEIIDCPIE DD KSYKSLER SIHGAVR KN G Y ID++N VR R+V KE+K
Sbjct: 746 LRVLEIIDCPIEWNDDVLKSYKSLERFSIHGAVRTKNQIGGYNIDYRNFVRSRKVKKEVK 803
Query: 781 TCYYY 786
TC YY
Sbjct: 806 TCVYY 803
BLAST of MELO3C009693 vs. NCBI nr
Match:
gi|449437958|ref|XP_004136757.1| (PREDICTED: putative disease resistance protein RGA3 [Cucumis sativus])
HSP 1 Score: 889.4 bits (2297), Expect = 4.5e-255
Identity = 485/790 (61.39%), Postives = 595/790 (75.32%), Query Frame = 1
Query: 1 MQYTMLSLKTILMDAE-KEEYSHHLNDWLQELQNVFSQIEGLLYEFNGKVKKQEATGKWV 60
++YTMLSLK+ILMDAE K+E S L +WL+ELQNVFSQIEG + E K + E GK V
Sbjct: 26 IEYTMLSLKSILMDAEEKQEQSRGLQNWLEELQNVFSQIEGFIDEH--KEEAYEGIGKQV 85
Query: 61 FV-LSFNSSRIEQTKKMMKLCDDLDEIASQMYDFNLTNM-------ETTHSFLSATEVST 120
S +S++I +T KM KL D L+E+A++MY+FNLT ETT+SFL+ATEVST
Sbjct: 86 LAPFSCSSNQIARTWKMEKLFDHLNEVAAKMYEFNLTERHTGAIKTETTNSFLTATEVST 145
Query: 121 RLMKPSWQLLYPLTNAPKVFHDKRYSNFLDHFKKSTLGFFHIVGEPGIGKTTLAKFFYND 180
RLMKPSW++LYPLTNAPK + D+RY L+ FK TLGFFHIVGE GIGK+TLAKF YND
Sbjct: 146 RLMKPSWKVLYPLTNAPKFYQDERYRKILNDFKNPTLGFFHIVGEAGIGKSTLAKFIYND 205
Query: 181 PEVVKTFPSRLWICVKEEFDPRRLIKEMLDFSHCQATYDNLTEKQLCFAVQQFLRDKTFL 240
PEV FPSRLW+CVKEEFD +RL+KE+L+FS+ AT DNLT K LC Q+LR++TFL
Sbjct: 206 PEVEGMFPSRLWVCVKEEFDTQRLMKEILNFSYSPATCDNLTTK-LC-PTDQYLRERTFL 265
Query: 241 LVFQDISIKNLGDCSIFKSLLGMGNPGSKIIVTTQNEEIAEAIRLGKLCK-------NES 300
LVFQD+SIKNL +CS+F SLL MG PGSKIIVTTQNEEIA AI L + K N S
Sbjct: 266 LVFQDLSIKNLDNCSLFTSLLMMGKPGSKIIVTTQNEEIANAIELTMIYKVGQQSEQNRS 325
Query: 301 QEVPSPEATEPSDVNNDEAY---------KDNMTYQKIFEVERLSKENSLSLFKVHAFT- 360
Q E ++VNN + + ++ Q IF+V+RLS+++SLSLFK +A T
Sbjct: 326 QTALDTVTKETANVNNADQFVQANPLGKIDQSIPSQTIFKVKRLSEKDSLSLFKDYASTY 385
Query: 361 ETQEAQIPNLTKIQEVIEQKCHGVPLAIKCLGGLLSKTSIA-EWNGVIDKLWEHEEGEDG 420
E E I K KC+G+PLAIKCLG +LS A +W + D E ++G++
Sbjct: 386 EGNEKDIMKTLK-------KCNGIPLAIKCLGSMLSLGPPATKW--MEDN--ERQKGDNE 445
Query: 421 NKSILPVLRLCYDRMPSHLQRCFLYCSQLTKDRILSSNDVIQLWIASDLL--PKENYLSL 480
+ S +L+LCY+ MPSHL+RCFLYCSQL D ILSSNDVIQLW+A+ LL +ENYLSL
Sbjct: 446 SSSTFSILKLCYNEMPSHLKRCFLYCSQLPNDSILSSNDVIQLWMANGLLRSRQENYLSL 505
Query: 481 EKIGENYFKELCSRCFLQELEEYGLGYWFKLHPLIEKLARILTQK-----QVFPVANTKC 540
E IGE YFKELCSRCFLQ++EEYGLGYWFK+HPLI +LAR++ ++ + PV N
Sbjct: 506 EDIGEIYFKELCSRCFLQDVEEYGLGYWFKMHPLIRELARLVQKRTKDLISIKPVTNVTS 565
Query: 541 IAFTVRDKVPPSAFLANTCIDKFKYIRLLYLGNANLREIPNAVEKLVQLRYLDLQGNKRL 600
IAF VRD+VP S+FLA CI KF+++RLLYLG+ +L+EIPN +E L L YLDLQGNK +
Sbjct: 566 IAFPVRDEVPSSSFLAEKCISKFQHLRLLYLGHTDLQEIPNTIETLNHLTYLDLQGNKNI 625
Query: 601 KRLPNSIFNLKNLQTLILASCSALEELPNHIKQLINLRYLWVTANNLRLHKNGVGTMTSL 660
KRLPN+I NL++LQTLILASCSALEELP I +L NLRYLWVT+N LRLHKNGVGTMTSL
Sbjct: 626 KRLPNAICNLQHLQTLILASCSALEELPKDICKLSNLRYLWVTSNKLRLHKNGVGTMTSL 685
Query: 661 RFLAIGGCENLQDLFEQPSCLVRLETLMIYDSKTLKLLPNEIGALISLKNLVIWSCKQLT 720
RFLAIGGC+ LQDLFE+PSCLVRLETLMIYD +L+LLPNE+G+LISL+NLVIWSCKQLT
Sbjct: 686 RFLAIGGCDKLQDLFERPSCLVRLETLMIYDCNSLQLLPNEMGSLISLQNLVIWSCKQLT 745
Query: 721 LT-LKGVEFRLRRFTIRELPRVRKLPEWIQRFTESLRVLEIIDCPIELKDDEFKSYKSLE 756
L L+ V+F L+RFTIRELP V KLPEW+QR TE+LRVLEIIDCPI+++++ K YK++E
Sbjct: 746 LKGLEKVDFSLQRFTIRELPEVNKLPEWLQRSTETLRVLEIIDCPIKVEEEGIKMYKAVE 800
BLAST of MELO3C009693 vs. NCBI nr
Match:
gi|778685015|ref|XP_004136758.2| (PREDICTED: putative disease resistance protein RGA1 [Cucumis sativus])
HSP 1 Score: 530.8 bits (1366), Expect = 4.1e-147
Identity = 293/545 (53.76%), Postives = 381/545 (69.91%), Query Frame = 1
Query: 246 SIFKSLLGMGNPGSKIIVTTQNEEIAEAIRLGKLCKNESQ---EVPSPEATEPSDV---- 305
S+ K+ PGS+I T + IA I L E EVP P+ TE +++
Sbjct: 310 SLTKNTQNQAVPGSQIEETA--DAIANKIYEHSLSLTEDAQNLEVPIPQVTEDTNIGLGI 369
Query: 306 NND--EAYKDNMTYQKIFEVERLSKENSLSLFKVHAFTETQEAQIPNLTKIQEVIEQKCH 365
N D +D+ YQ IF++E+LSK++S LFK +AF QE + P LTKI + + +KC
Sbjct: 370 NPDIPPIKQDDTEYQTIFKLEKLSKQSSSRLFKEYAFRNRQEVENPELTKIVDQLLEKCM 429
Query: 366 GVPLAIKCLGGLLS-KTSIAEWNGVIDKLWEHEEGEDGNKSILPVLRLCYDRMPSHLQRC 425
GVPLAIKCLG LLS +TSIA+W + +KL E+ E+G IL VLR+CYD+MPS L+ C
Sbjct: 430 GVPLAIKCLGSLLSSETSIAKWKNIEEKLSLQEKKENG---ILHVLRVCYDQMPSQLKPC 489
Query: 426 FLYCSQLTKDRILSSNDVIQLWIASDLL--PKENYLSLEKIGENYFKELCSRCFLQELEE 485
FL+CSQL DRI+SSND+IQLW+A+ LL P+E ++E IGE YF EL SR F+QE+EE
Sbjct: 490 FLHCSQLPNDRIISSNDMIQLWMANGLLHSPEEKNSTMENIGEKYFMELWSRYFIQEIEE 549
Query: 486 YGLGYWFKLHPLIEKLARILTQKQVF---------PVANTKCIAFTVRDKVPPSAFLANT 545
+GLGYW KLHPLI+KLA +TQ+Q V + IAF R+ V P+A L
Sbjct: 550 HGLGYWIKLHPLIQKLAHKITQEQSEGSGGNNHPKEVTEIRSIAFQERNMVLPNASLTEK 609
Query: 546 CIDKFKYIRLLYLGNANLREIPNAVEKLVQLRYLDLQGNKRLKRLPNSIFNLKNLQTLIL 605
CI K+K +RLLYL NA+L+EIPN++ L LRYLDL GN ++K LPNSI NL++LQTLIL
Sbjct: 610 CIWKYKGLRLLYLSNADLQEIPNSIGTLKYLRYLDLHGNTKIKHLPNSICNLQSLQTLIL 669
Query: 606 ASCSALEELPNHIKQLINLRYLWVTANNLRLHKNGVGTMTSLRFLAIGGCENLQDLFEQP 665
SCSALE+LP I+ LI+LRYLWVT N LRL KNGVGTM SLRFLAIGGC NL++LFE+P
Sbjct: 670 GSCSALEDLPKDIRNLISLRYLWVTTNKLRLDKNGVGTMNSLRFLAIGGCNNLENLFERP 729
Query: 666 SCLVRLETLMIYDSKTLKLLPNEIGALISLKNLVIWSCKQLTLTLKGVEFRLRRFTIREL 725
CL LETLMIY+ TLKLLP+E+ L SL+NL+IWSCKQLTL LK VEF+L+RFTI+EL
Sbjct: 730 DCLAGLETLMIYNCTTLKLLPDEMRYLKSLQNLMIWSCKQLTLNLKEVEFKLQRFTIKEL 789
Query: 726 PRVRKLPEWIQRFTESLRVLEIIDCPIELKDDE-FKSYKSLERLSIHGAVRIKNLNGEYC 769
PRV +LP+W++ E+LR L+II+CPI + + + + Y+++E I+GAVR + Y
Sbjct: 790 PRVERLPQWLENSAETLRTLQIINCPIRIMERQGIEKYEAVENTIIYGAVRFEMAPPGYD 849
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| RGA1_SOLBU | 2.1e-58 | 29.09 | Putative disease resistance protein RGA1 OS=Solanum bulbocastanum GN=RGA1 PE=2 S... | [more] |
| RGA4_SOLBU | 3.3e-56 | 28.44 | Putative disease resistance protein RGA4 OS=Solanum bulbocastanum GN=RGA4 PE=2 S... | [more] |
| RGA2_SOLBU | 3.4e-53 | 27.86 | Disease resistance protein RGA2 OS=Solanum bulbocastanum GN=RGA2 PE=1 SV=1 | [more] |
| RGA3_SOLBU | 9.3e-43 | 27.23 | Putative disease resistance protein RGA3 OS=Solanum bulbocastanum GN=RGA3 PE=2 S... | [more] |
| R13L1_ARATH | 2.6e-37 | 24.44 | Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana GN=RPPL... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A0A0LF99_CUCSA | 0.0e+00 | 86.24 | Uncharacterized protein OS=Cucumis sativus GN=Csa_3G814390 PE=3 SV=1 | [more] |
| A0A0A0LEM8_CUCSA | 3.1e-255 | 61.39 | Uncharacterized protein OS=Cucumis sativus GN=Csa_3G814400 PE=3 SV=1 | [more] |
| A0A0A0LBH9_CUCSA | 2.8e-147 | 53.76 | Uncharacterized protein OS=Cucumis sativus GN=Csa_3G815400 PE=3 SV=1 | [more] |
| A0A0A0LBH9_CUCSA | 3.2e-58 | 45.52 | Uncharacterized protein OS=Cucumis sativus GN=Csa_3G815400 PE=3 SV=1 | [more] |
| B9I533_POPTR | 5.4e-90 | 32.69 | NBS-LRR resistance gene-like protein ARGH30 OS=Populus trichocarpa GN=POPTR_0012... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| AT3G14470.1 | 1.5e-38 | 24.44 | NB-ARC domain-containing disease resistance protein | [more] |
| AT3G50950.2 | 1.9e-33 | 22.92 | HOPZ-ACTIVATED RESISTANCE 1 | [more] |
| AT3G14460.1 | 2.8e-29 | 27.81 | LRR and NB-ARC domains-containing disease resistance protein | [more] |
| AT4G27220.1 | 3.2e-25 | 24.60 | NB-ARC domain-containing disease resistance protein | [more] |
| AT1G59780.1 | 2.3e-23 | 23.18 | NB-ARC domain-containing disease resistance protein | [more] |
