MELO3C008895 (gene) Melon (DHL92) v3.5.1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	MELO3C008895
Type	gene
Organism	Cucumis melo (Melon (DHL92) v3.5.1)
Description	Cysteine proteinase inhibitor (Cystatin)
Location	chr8 : 22699100 .. 22699806 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AATATATGTGACGTTGGAAACGAACAATTATATAGAAATACCAAACTTCTGAATATTAATATCATATTTGAAAGGTAAATTAATGAAGGATTAGAAAATGTTTAGCCACCAAAGGTACTGTATAATGAGTGTTGAAGAAGCATATCACACTTCGAAACCTATTGAGAATATAAATGATGATCTGCAAGTGCAGTCAATAATAGGAAAGTTGGCTGTTGAAGACCATAACAAGAAAACAGGAGAGAATTTGGAGTTTGTGGATGTGGTGAATGGTTTGAGAAGTGAAGTTTTTGTTCAACCTGGGTGTACTCATGAAGGAATATTATATCATCTTTTGGTGGAGGCTAAAACCATAGAAGGAATTAATTGGACATATGTGGCCAAGTTGTTAGAGCTTTACGTAGGCTGCCGAATAAGGTATGAATTCAAATCTTTTGAACCTTTTCTCCCATACTACAACCCCTAATATTACCCCTCATCTCTCTACCTACTTGTCACATGCTAATTAAATAAATTGTATCTTTTAATAATATCGTATATCAAAAATGTTTGCACCATAGTTGCATTTAGACTTCTCAAATTGGACCCTCCAACTTATATAATCAATGCGATTTATACAATTATATAAGTATGAGCATCCAATTTTGAATTTTTAATGTATTTGAGAATAGTATAATTTTGTTTTCTAATCTTCAATTTGTGCATTC

mRNA sequence

AATATATGTGACGTTGGAAACGAACAATTATATAGAAATACCAAACTTCTGAATATTAATATCATATTTGAAAGGTAAATTAATGAAGGATTAGAAAATGTTTAGCCACCAAAGGTACTGTATAATGAGTGTTGAAGAAGCATATCACACTTCGAAACCTATTGAGAATATAAATGATGATCTGCAAGTGCAGTCAATAATAGGAAAGTTGGCTGTTGAAGACCATAACAAGAAAACAGGAGAGAATTTGGAGTTTGTGGATGTGGTGAATGGTTTGAGAAGTGAAGTTTTTGTTCAACCTGGGTGTACTCATGAAGGAATATTATATCATCTTTTGGTGGAGGCTAAAACCATAGAAGGAATTAATTGGACATATGTGGCCAAGTTGTTAGAGCTTTACGTAGGCTGCCGAATAAGGTATGAATTCAAATCTTTTGAACCTTTTCTCCCATACTACAACCCCTAATATTACCCCTCATCTCTCTACCTACTTGTCACATGCTAATTAAATAAATTGTATCTTTTAATAATATCGTATATCAAAAATGTTTGCACCATAGTTGCATTTAGACTTCTCAAATTGGACCCTCCAACTTATATAATCAATGCGATTTATACAATTATATAAGTATGAGCATCCAATTTTGAATTTTTAATGTATTTGAGAATAGTATAATTTTGTTTTCTAATCTTCAATTTGTGCATTC

Coding sequence (CDS)

ATGTTTAGCCACCAAAGGTACTGTATAATGAGTGTTGAAGAAGCATATCACACTTCGAAACCTATTGAGAATATAAATGATGATCTGCAAGTGCAGTCAATAATAGGAAAGTTGGCTGTTGAAGACCATAACAAGAAAACAGGAGAGAATTTGGAGTTTGTGGATGTGGTGAATGGTTTGAGAAGTGAAGTTTTTGTTCAACCTGGGTGTACTCATGAAGGAATATTATATCATCTTTTGGTGGAGGCTAAAACCATAGAAGGAATTAATTGGACATATGTGGCCAAGTTGTTAGAGCTTTACGTAGGCTGCCGAATAAGGTATGAATTCAAATCTTTTGAACCTTTTCTCCCATACTACAACCCCTAA

Protein sequence

MFSHQRYCIMSVEEAYHTSKPIENINDDLQVQSIIGKLAVEDHNKKTGENLEFVDVVNGLRSEVFVQPGCTHEGILYHLLVEAKTIEGINWTYVAKLLELYVGCRIRYEFKSFEPFLPYYNP*

Homology

[Close]

BLAST of MELO3C008895 vs. TrEMBL
Match: A0A0A0L3W1_CUCSA (Proteinase inhibitor I25, cystatin OS=Cucumis sativus GN=Csa_4G508490 PE=4 SV=1)

HSP 1 Score: 239.6 bits (610), Expect = 2.0e-60
Identity = 114/122 (93.44%), Postives = 118/122 (96.72%), Query Frame = 1


Query: 1   MFSHQRYCIMSVEEAYHTSKPIENINDDLQVQSIIGKLAVEDHNKKTGENLEFVDVVNGL 60
           MFSHQRYCIMSVEEAYHTSKPIE+INDDLQVQSIIG+LAVEDHNKKTG+NLE VDVVNGL
Sbjct: 1   MFSHQRYCIMSVEEAYHTSKPIEDINDDLQVQSIIGQLAVEDHNKKTGDNLELVDVVNGL 60

Query: 61  RSEVFVQPGCTHEGILYHLLVEAKTIEGINWTYVAKLLELYVGCRIRYEFKSFEPFLPYY 120
           RS +FVQPG THEGILYHLLVEAKTIEGINWTYVAKLLELYVGCRIRYEFKSFEP LPYY
Sbjct: 61  RSGIFVQPGSTHEGILYHLLVEAKTIEGINWTYVAKLLELYVGCRIRYEFKSFEPLLPYY 120

Query: 121 NP 123
           NP
Sbjct: 121 NP 122

[Close]

BLAST of MELO3C008895 vs. TrEMBL
Match: A0A0A0L191_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G508520 PE=4 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 2.1e-25
Identity = 69/114 (60.53%), Postives = 83/114 (72.81%), Query Frame = 1


Query: 10  MSVEEAYHTSKPIENINDDLQVQSIIGKLAVEDHNKKT-GENLEFVDVVNGLRSEVFVQP 69
           MS+EE +H  KPI +IND   VQ IIGKLAVEDH+    GE L+FV VVNGL+S+ ++ P
Sbjct: 1   MSIEEIHHRLKPIIDINDP-HVQ-IIGKLAVEDHDSHNPGEKLKFVRVVNGLKSDKYIGP 60

Query: 70  GCTHEGILYHLLVEAKTIEGINWTYVAKLLELYVGCRIRYEFKSFEPFLPYYNP 123
           G T E ILYHL++EAKT E INWTY  KL ++Y GC IR+ F SFEP LPY  P
Sbjct: 61  GFT-EVILYHLVLEAKTNEEINWTYATKLEKVYSGCLIRHVFLSFEPVLPYNKP 111

[Close]

BLAST of MELO3C008895 vs. TrEMBL
Match: A0A0A0KZ98_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G508510 PE=4 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 1.1e-21
Identity = 65/114 (57.02%), Postives = 84/114 (73.68%), Query Frame = 1


Query: 10  MSVEEAYHTSKPIENINDDLQVQSIIGKLAVED-HNKKTGENLEFVDVVNGLRSEVFVQP 69
           MS+EE+ H  KPI +IND   VQ++ G+LAVE+ ++K +GE L+FV VVNGL+S+ ++ P
Sbjct: 1   MSIEESRHRLKPIIDINDP-HVQAL-GELAVENLYSKNSGEKLKFVRVVNGLKSDKYIGP 60

Query: 70  GCTHEGILYHLLVEAKTIEGINWTYVAKLLELYVGCRIRYEFKSFEPFLPYYNP 123
           G T E ILYHL++EAKT E INWTY  KL E+  G  IR+ F SFEP LPYY P
Sbjct: 61  GFT-EVILYHLVLEAKTNEEINWTYATKLEEVSGGL-IRHVFLSFEPVLPYYKP 110

[Close]

BLAST of MELO3C008895 vs. NCBI nr
Match: gi|700199663|gb|KGN54821.1| (Proteinase inhibitor I25, cystatin [Cucumis sativus])

HSP 1 Score: 239.6 bits (610), Expect = 2.9e-60
Identity = 114/122 (93.44%), Postives = 118/122 (96.72%), Query Frame = 1


Query: 1   MFSHQRYCIMSVEEAYHTSKPIENINDDLQVQSIIGKLAVEDHNKKTGENLEFVDVVNGL 60
           MFSHQRYCIMSVEEAYHTSKPIE+INDDLQVQSIIG+LAVEDHNKKTG+NLE VDVVNGL
Sbjct: 1   MFSHQRYCIMSVEEAYHTSKPIEDINDDLQVQSIIGQLAVEDHNKKTGDNLELVDVVNGL 60

Query: 61  RSEVFVQPGCTHEGILYHLLVEAKTIEGINWTYVAKLLELYVGCRIRYEFKSFEPFLPYY 120
           RS +FVQPG THEGILYHLLVEAKTIEGINWTYVAKLLELYVGCRIRYEFKSFEP LPYY
Sbjct: 61  RSGIFVQPGSTHEGILYHLLVEAKTIEGINWTYVAKLLELYVGCRIRYEFKSFEPLLPYY 120

Query: 121 NP 123
           NP
Sbjct: 121 NP 122

[Close]

BLAST of MELO3C008895 vs. NCBI nr
Match: gi|700199666|gb|KGN54824.1| (hypothetical protein Csa_4G508520 [Cucumis sativus])

HSP 1 Score: 123.2 bits (308), Expect = 3.1e-25
Identity = 69/114 (60.53%), Postives = 83/114 (72.81%), Query Frame = 1


Query: 10  MSVEEAYHTSKPIENINDDLQVQSIIGKLAVEDHNKKT-GENLEFVDVVNGLRSEVFVQP 69
           MS+EE +H  KPI +IND   VQ IIGKLAVEDH+    GE L+FV VVNGL+S+ ++ P
Sbjct: 1   MSIEEIHHRLKPIIDINDP-HVQ-IIGKLAVEDHDSHNPGEKLKFVRVVNGLKSDKYIGP 60

Query: 70  GCTHEGILYHLLVEAKTIEGINWTYVAKLLELYVGCRIRYEFKSFEPFLPYYNP 123
           G T E ILYHL++EAKT E INWTY  KL ++Y GC IR+ F SFEP LPY  P
Sbjct: 61  GFT-EVILYHLVLEAKTNEEINWTYATKLEKVYSGCLIRHVFLSFEPVLPYNKP 111

[Close]

BLAST of MELO3C008895 vs. NCBI nr
Match: gi|700199665|gb|KGN54823.1| (hypothetical protein Csa_4G508510 [Cucumis sativus])

HSP 1 Score: 110.9 bits (276), Expect = 1.6e-21
Identity = 65/114 (57.02%), Postives = 84/114 (73.68%), Query Frame = 1


Query: 10  MSVEEAYHTSKPIENINDDLQVQSIIGKLAVED-HNKKTGENLEFVDVVNGLRSEVFVQP 69
           MS+EE+ H  KPI +IND   VQ++ G+LAVE+ ++K +GE L+FV VVNGL+S+ ++ P
Sbjct: 1   MSIEESRHRLKPIIDINDP-HVQAL-GELAVENLYSKNSGEKLKFVRVVNGLKSDKYIGP 60

Query: 70  GCTHEGILYHLLVEAKTIEGINWTYVAKLLELYVGCRIRYEFKSFEPFLPYYNP 123
           G T E ILYHL++EAKT E INWTY  KL E+  G  IR+ F SFEP LPYY P
Sbjct: 61  GFT-EVILYHLVLEAKTNEEINWTYATKLEEVSGGL-IRHVFLSFEPVLPYYKP 110

[Close]

BLAST of MELO3C008895 vs. NCBI nr
Match: gi|659083303|ref|XP_008442277.1| (PREDICTED: cysteine proteinase inhibitor A-like [Cucumis melo])

HSP 1 Score: 94.0 bits (232), Expect = 2.0e-16
Identity = 56/113 (49.56%), Postives = 73/113 (64.60%), Query Frame = 1


Query: 10  MSVEEAYHTSKPIENINDDLQVQSIIGKLAVEDHNKKTGENLEFVDVVNGLRSEVFVQPG 69
           MS+  A+   KPIE+IND+L+VQ + G L V+DHNKKTGENL+FV VVNGL +  F  P 
Sbjct: 1   MSIMSAF---KPIEDINDNLEVQDL-GNLTVDDHNKKTGENLKFVRVVNGLAAS-FSDPI 60

Query: 70  CTHEGILYHLLVEAKTIEGINWTY---VAKLLELYVGCRIRYEFKSFEPFLPY 120
               G+LY L++EA T   INWTY   V+K   +     + Y F+SF+P L Y
Sbjct: 61  SPLGGVLYELVLEAITTSKINWTYKARVSKSKAITPPFHMLYSFQSFKPVLEY 108

The following BLAST results are available for this feature:

BLAST of MELO3C008895 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L3W1_CUCSA	2.0e-60	93.44	Proteinase inhibitor I25, cystatin OS=Cucumis sativus GN=Csa_4G508490 PE=4 SV=1	[more]
A0A0A0L191_CUCSA	2.1e-25	60.53	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G508520 PE=4 SV=1	[more]
A0A0A0KZ98_CUCSA	1.1e-21	57.02	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G508510 PE=4 SV=1	[more]

BLAST of MELO3C008895 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700199663\|gb\|KGN54821.1\|	2.9e-60	93.44	Proteinase inhibitor I25, cystatin [Cucumis sativus]	[more]
gi\|700199666\|gb\|KGN54824.1\|	3.1e-25	60.53	hypothetical protein Csa_4G508520 [Cucumis sativus]	[more]
gi\|700199665\|gb\|KGN54823.1\|	1.6e-21	57.02	hypothetical protein Csa_4G508510 [Cucumis sativus]	[more]
gi\|659083303\|ref\|XP_008442277.1\|	2.0e-16	49.56	PREDICTED: cysteine proteinase inhibitor A-like [Cucumis melo]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR020381	Proteinase inhibitor I25, cystatin, conserved region

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
biological_process	GO:0010951	negative regulation of endopeptidase activity
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity

Unigene

This gene is associated with the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
MU51115	melon EST collection version 4.0	transcribed_cluster

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MELO3C008895T1	MELO3C008895T1	mRNA

The following transcribed_cluster feature(s) are associated with this gene:


Feature Name	Unique Name	Type
MU51115	MU51115	transcribed_cluster

InterPro

Analysis Name: InterPro Annotations of melon
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR020381	Proteinase inhibitor I25, cystatin, conserved region	PFAM	PF16845	SQAPI	coord: 22..100 score: 1.
None	No IPR available	GENE3D	G3DSA:3.10.450.10		coord: 20..91 score: 8.
None	No IPR available	unknown	SSF54403	Cystatin/monellin	coord: 23..101 score: 3.16

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
MELO3C008895	Csa4G508490	Cucumber (Chinese Long) v2	cumeB326
MELO3C008895	Cla003015	Watermelon (97103) v1	mewmB576
MELO3C008895	ClCG04G002440	Watermelon (Charleston Gray)	mewcgB526
MELO3C008895	Cla97C04G069980	Watermelon (97103) v2	mewmbB534
MELO3C008895	Bhi03G002318	Wax gourd	mewgoB656
MELO3C008895	CSPI04G20140	Wild cucumber (PI 183967)	cpimeB334
MELO3C008895	Cucsa.264120	Cucumber (Gy14) v1	cgymeB444
MELO3C008895	Lsi01G020650	Bottle gourd (USVL1VR-Ls)	lsimeB159
MELO3C008895	CsGy4G018770	Cucumber (Gy14) v2	cgybmeB285

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
MELO3C008895	Watermelon (Charleston Gray)	mewcgB533
MELO3C008895	Watermelon (97103) v1	mewmB587
MELO3C008895	Cucurbita pepo (Zucchini)	cpemeB070
MELO3C008895	Cucurbita pepo (Zucchini)	cpemeB074
MELO3C008895	Cucurbita pepo (Zucchini)	cpemeB459
MELO3C008895	Bottle gourd (USVL1VR-Ls)	lsimeB157
MELO3C008895	Silver-seed gourd	carmeB0286
MELO3C008895	Silver-seed gourd	carmeB0586
MELO3C008895	Silver-seed gourd	carmeB0998
MELO3C008895	Cucumber (Chinese Long) v3	cucmeB336
MELO3C008895	Cucumber (Chinese Long) v3	cucmeB339
MELO3C008895	Watermelon (97103) v2	mewmbB542
MELO3C008895	Cucumber (Gy14) v1	cgymeB047
MELO3C008895	Cucurbita maxima (Rimu)	cmameB610
MELO3C008895	Cucurbita maxima (Rimu)	cmameB620
MELO3C008895	Cucurbita maxima (Rimu)	cmameB801
MELO3C008895	Cucurbita maxima (Rimu)	cmameB806
MELO3C008895	Cucurbita moschata (Rifu)	cmomeB599
MELO3C008895	Cucurbita moschata (Rifu)	cmomeB612
MELO3C008895	Cucurbita moschata (Rifu)	cmomeB790
MELO3C008895	Cucurbita moschata (Rifu)	cmomeB785
MELO3C008895	Wild cucumber (PI 183967)	cpimeB335