MELO3C003855 (gene) Melon (DHL92) v3.5.1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	MELO3C003855
Type	gene
Organism	Cucumis melo (Melon (DHL92) v3.5.1)
Description	Cytoplasmic tRNA 2-thiolation protein 1
Location	chr4 : 5596540 .. 5596825 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATTTTTTTTTTCTCCTTAAGGCTCTTGACAGAGGTGTTGCATTGTTGAAAGTAGTCAAGCTAGCCACAGGGCACAATGCAATTGATATGGTTGGAACAATTCTATTAAATATATTACGAGGTGATATTCCAAGGTATTTTAATAGTAAGACTTTTTATTTCTGTCAATTACTTTTGTCCCTCATTTGGGTTGTATTAGACTGAAGTGATGCTTTGTATTTGTAGATTGAGTAGATGTACTGCATTAATTACCGACGAAGATGGACCAAATCCAAGATGCTAA

mRNA sequence

ATGGATTTTTTTTTTCTCCTTAAGGCTCTTGACAGAGGTGTTGCATTGTTGAAAGTAGTCAAGCTAGCCACAGGGCACAATGCAATTGATATGGTTGGAACAATTCTATTAAATATATTACGAGGTGATATTCCAAGATTGAGTAGATGTACTGCATTAATTACCGACGAAGATGGACCAAATCCAAGATGCTAA

Coding sequence (CDS)

ATGGATTTTTTTTTTCTCCTTAAGGCTCTTGACAGAGGTGTTGCATTGTTGAAAGTAGTCAAGCTAGCCACAGGGCACAATGCAATTGATATGGTTGGAACAATTCTATTAAATATATTACGAGGTGATATTCCAAGATTGAGTAGATGTACTGCATTAATTACCGACGAAGATGGACCAAATCCAAGATGCTAA

Protein sequence

MDFFFLLKALDRGVALLKVVKLATGHNAIDMVGTILLNILRGDIPRLSRCTALITDEDGPNPRC*

Homology

[Close]

BLAST of MELO3C003855 vs. Swiss-Prot
Match: CTU1_ARATH (Cytoplasmic tRNA 2-thiolation protein 1 OS=Arabidopsis thaliana GN=NCS6 PE=2 SV=2)

HSP 1 Score: 88.2 bits (217), Expect = 3.6e-17
Identity = 43/60 (71.67%), Postives = 46/60 (76.67%), Query Frame = 1


Query: 5   FLLKALDRGVALLKVVKLATGHNAIDMVGTILLNILRGDIPRLSRCTALITDEDGPNPRC 64
           F  +ALDRG ALLKV KL TGHNA D+  T+LLNILRGDI RLSRCT++ T EDGP PRC
Sbjct: 155 FRRQALDRGAALLKVEKLVTGHNADDIAETVLLNILRGDIARLSRCTSITTGEDGPIPRC 214

[Close]

BLAST of MELO3C003855 vs. Swiss-Prot
Match: CTU1_ORYSJ (Cytoplasmic tRNA 2-thiolation protein 1 OS=Oryza sativa subsp. japonica GN=NCS6 PE=2 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 1.8e-16
Identity = 42/60 (70.00%), Postives = 45/60 (75.00%), Query Frame = 1


Query: 5   FLLKALDRGVALLKVVKLATGHNAIDMVGTILLNILRGDIPRLSRCTALITDEDGPNPRC 64
           F  +ALDRG ALLKV K+ TGHNA D+  T+LLNILRGDI RLSRCT + T EDGP PRC
Sbjct: 161 FRRQALDRGAALLKVDKIVTGHNADDIAETVLLNILRGDIARLSRCTFITTGEDGPIPRC 220

[Close]

BLAST of MELO3C003855 vs. Swiss-Prot
Match: CTU1_CHLRE (Cytoplasmic tRNA 2-thiolation protein 1 OS=Chlamydomonas reinhardtii GN=NCS6 PE=3 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 1.2e-12
Identity = 35/59 (59.32%), Postives = 40/59 (67.80%), Query Frame = 1


Query: 5   FLLKALDRGVALLKVVKLATGHNAIDMVGTILLNILRGDIPRLSRCTALITDEDGPNPR 64
           F  +ALDRG  L+   K+ATGHNA D+  T+LLNILRGD+PRL RC  +IT ED   PR
Sbjct: 147 FRRQALDRGAVLMGANKIATGHNADDVAETVLLNILRGDVPRLGRCANIITGEDSSLPR 205

[Close]

BLAST of MELO3C003855 vs. Swiss-Prot
Match: CTU1_DICDI (Cytoplasmic tRNA 2-thiolation protein 1 OS=Dictyostelium discoideum GN=ctu1 PE=2 SV=2)

HSP 1 Score: 72.0 bits (175), Expect = 2.7e-12
Identity = 33/59 (55.93%), Postives = 40/59 (67.80%), Query Frame = 1


Query: 5   FLLKALDRGVALLKVVKLATGHNAIDMVGTILLNILRGDIPRLSRCTALITDEDGPNPR 64
           F  +ALDRG  +LK  K+ TGHNA D+  T+L+N+LRGDIPRL RC  +IT  +G  PR
Sbjct: 154 FRRQALDRGAVMLKANKIVTGHNADDIAETVLMNLLRGDIPRLQRCVNIITGSEGALPR 212

[Close]

BLAST of MELO3C003855 vs. Swiss-Prot
Match: CTU1_XENLA (Cytoplasmic tRNA 2-thiolation protein 1 OS=Xenopus laevis GN=ctu1 PE=2 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 3.5e-12
Identity = 33/60 (55.00%), Postives = 40/60 (66.67%), Query Frame = 1


Query: 5   FLLKALDRGVALLKVVKLATGHNAIDMVGTILLNILRGDIPRLSRCTALITDEDGPNPRC 64
           F  +ALDRG  +L + K+ TGHNA D+  T+L+N LRGDI RL RCTA+ T  +G  PRC
Sbjct: 146 FRRQALDRGAMMLGINKICTGHNADDIAETVLMNFLRGDIARLRRCTAITTGSEGAIPRC 205

[Close]

BLAST of MELO3C003855 vs. TrEMBL
Match: A0A0A0LKF0_CUCSA (Cytoplasmic tRNA 2-thiolation protein 1 OS=Cucumis sativus GN=NCS6 PE=3 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 7.3e-17
Identity = 47/60 (78.33%), Postives = 50/60 (83.33%), Query Frame = 1


Query: 5   FLLKALDRGVALLKVVKLATGHNAIDMVGTILLNILRGDIPRLSRCTALITDEDGPNPRC 64
           F  +ALDRG ALLKV KLATGHNA DM  T+LLNILRGDI RLSRCTA+IT EDGP PRC
Sbjct: 157 FRRQALDRGAALLKVDKLATGHNADDMAETVLLNILRGDIARLSRCTAIITGEDGPIPRC 216

[Close]

BLAST of MELO3C003855 vs. TrEMBL
Match: A0A0D3D124_BRAOL (Uncharacterized protein OS=Brassica oleracea var. oleracea PE=4 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 9.6e-17
Identity = 45/62 (72.58%), Postives = 50/62 (80.65%), Query Frame = 1


Query: 3   FFFLLKALDRGVALLKVVKLATGHNAIDMVGTILLNILRGDIPRLSRCTALITDEDGPNP 62
           F F +KALDRG ALLKV KL TGHNA D+  T+LLNILRGDI RLSRCT++ T EDGP P
Sbjct: 130 FMFFVKALDRGAALLKVDKLVTGHNADDIAETVLLNILRGDIARLSRCTSITTGEDGPIP 189

Query: 63  RC 65
           RC
Sbjct: 190 RC 191

[Close]

BLAST of MELO3C003855 vs. TrEMBL
Match: A0A103XR73_CYNCS (Rossmann-like alpha/beta/alpha sandwich fold (Fragment) OS=Cynara cardunculus var. scolymus GN=Ccrd_002507 PE=4 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 4.8e-16
Identity = 45/60 (75.00%), Postives = 50/60 (83.33%), Query Frame = 1


Query: 5   FLLKALDRGVALLKVVKLATGHNAIDMVGTILLNILRGDIPRLSRCTALITDEDGPNPRC 64
           F  +ALDRG ALLKV KLATGHNA D+  T+LLNILRGDI RLSRCT++IT EDGP PRC
Sbjct: 215 FRRQALDRGAALLKVDKLATGHNADDIAETVLLNILRGDIARLSRCTSIITGEDGPIPRC 274

[Close]

BLAST of MELO3C003855 vs. TrEMBL
Match: M5XB75_PRUPE (Cytoplasmic tRNA 2-thiolation protein 1 OS=Prunus persica GN=NCS6 PE=3 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 6.2e-16
Identity = 44/60 (73.33%), Postives = 50/60 (83.33%), Query Frame = 1


Query: 5   FLLKALDRGVALLKVVKLATGHNAIDMVGTILLNILRGDIPRLSRCTALITDEDGPNPRC 64
           F  +ALDRG ALLKV KLATGHNA D+  T+LLNILRGDI RLSRCT+++T EDGP PRC
Sbjct: 158 FRRQALDRGAALLKVDKLATGHNADDIAETVLLNILRGDIARLSRCTSIVTGEDGPIPRC 217

[Close]

BLAST of MELO3C003855 vs. TrEMBL
Match: E0CQT4_VITVI (Cytoplasmic tRNA 2-thiolation protein 1 OS=Vitis vinifera GN=NCS6 PE=3 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 8.1e-16
Identity = 44/60 (73.33%), Postives = 50/60 (83.33%), Query Frame = 1


Query: 5   FLLKALDRGVALLKVVKLATGHNAIDMVGTILLNILRGDIPRLSRCTALITDEDGPNPRC 64
           F  +ALDRG A+LKV KLATGHNA D+  T+LLNILRGDI RLSRCT++IT EDGP PRC
Sbjct: 157 FRRQALDRGAAMLKVDKLATGHNADDIAETVLLNILRGDIARLSRCTSIITGEDGPIPRC 216

[Close]

BLAST of MELO3C003855 vs. TAIR10
Match: AT2G44270.2 (AT2G44270.2 repressor of lrx1)

HSP 1 Score: 88.2 bits (217), Expect = 2.0e-18
Identity = 43/60 (71.67%), Postives = 46/60 (76.67%), Query Frame = 1


Query: 5   FLLKALDRGVALLKVVKLATGHNAIDMVGTILLNILRGDIPRLSRCTALITDEDGPNPRC 64
           F  +ALDRG ALLKV KL TGHNA D+  T+LLNILRGDI RLSRCT++ T EDGP PRC
Sbjct: 165 FRRQALDRGAALLKVEKLVTGHNADDIAETVLLNILRGDIARLSRCTSITTGEDGPIPRC 224

[Close]

BLAST of MELO3C003855 vs. TAIR10
Match: AT1G76170.1 (AT1G76170.1 2-thiocytidine tRNA biosynthesis protein, TtcA)

HSP 1 Score: 86.7 bits (213), Expect = 5.9e-18
Identity = 42/60 (70.00%), Postives = 46/60 (76.67%), Query Frame = 1


Query: 5   FLLKALDRGVALLKVVKLATGHNAIDMVGTILLNILRGDIPRLSRCTALITDEDGPNPRC 64
           F  +ALDRG ALLKV KL TGHNA D+  T++LNILRGDI RLSRCT++ T EDGP PRC
Sbjct: 133 FRRQALDRGAALLKVDKLVTGHNADDIAETVVLNILRGDIARLSRCTSITTGEDGPIPRC 192

[Close]

BLAST of MELO3C003855 vs. NCBI nr
Match: gi|659093684|ref|XP_008447661.1| (PREDICTED: cytoplasmic tRNA 2-thiolation protein 1-like [Cucumis melo])

HSP 1 Score: 101.7 bits (252), Expect = 5.0e-19
Identity = 51/56 (91.07%), Postives = 52/56 (92.86%), Query Frame = 1


Query: 9   ALDRGVALLKVVKLATGHNAIDMVGTILLNILRGDIPRLSRCTALITDEDGPNPRC 65
           ALDR VALLKVV+LATGHNAIDMVGTILLNILRGDI RLSRCT LITDEDGP PRC
Sbjct: 80  ALDRVVALLKVVRLATGHNAIDMVGTILLNILRGDIARLSRCTKLITDEDGPIPRC 135

[Close]

BLAST of MELO3C003855 vs. NCBI nr
Match: gi|778670437|ref|XP_011649473.1| (PREDICTED: cytoplasmic tRNA 2-thiolation protein 1 [Cucumis sativus])

HSP 1 Score: 94.0 bits (232), Expect = 1.1e-16
Identity = 47/60 (78.33%), Postives = 50/60 (83.33%), Query Frame = 1


Query: 5   FLLKALDRGVALLKVVKLATGHNAIDMVGTILLNILRGDIPRLSRCTALITDEDGPNPRC 64
           F  +ALDRG ALLKV KLATGHNA DM  T+LLNILRGDI RLSRCTA+IT EDGP PRC
Sbjct: 157 FRRQALDRGAALLKVDKLATGHNADDMAETVLLNILRGDIARLSRCTAIITGEDGPIPRC 216

[Close]

BLAST of MELO3C003855 vs. NCBI nr
Match: gi|659089053|ref|XP_008445303.1| (PREDICTED: cytoplasmic tRNA 2-thiolation protein 1 [Cucumis melo])

HSP 1 Score: 91.7 bits (226), Expect = 5.2e-16
Identity = 46/60 (76.67%), Postives = 49/60 (81.67%), Query Frame = 1


Query: 5   FLLKALDRGVALLKVVKLATGHNAIDMVGTILLNILRGDIPRLSRCTALITDEDGPNPRC 64
           F  +ALDRG A LKV KLATGHNA DM  T+LLNILRGDI RLSRCTA+IT EDGP PRC
Sbjct: 157 FRRQALDRGAAWLKVDKLATGHNADDMAETVLLNILRGDIARLSRCTAIITGEDGPIPRC 216

[Close]

BLAST of MELO3C003855 vs. NCBI nr
Match: gi|976908178|gb|KVH95408.1| (Rossmann-like alpha/beta/alpha sandwich fold, partial [Cynara cardunculus var. scolymus])

HSP 1 Score: 91.3 bits (225), Expect = 6.8e-16
Identity = 45/60 (75.00%), Postives = 50/60 (83.33%), Query Frame = 1


Query: 5   FLLKALDRGVALLKVVKLATGHNAIDMVGTILLNILRGDIPRLSRCTALITDEDGPNPRC 64
           F  +ALDRG ALLKV KLATGHNA D+  T+LLNILRGDI RLSRCT++IT EDGP PRC
Sbjct: 215 FRRQALDRGAALLKVDKLATGHNADDIAETVLLNILRGDIARLSRCTSIITGEDGPIPRC 274

[Close]

BLAST of MELO3C003855 vs. NCBI nr
Match: gi|596130726|ref|XP_007222158.1| (hypothetical protein PRUPE_ppa007715mg [Prunus persica])

HSP 1 Score: 90.9 bits (224), Expect = 8.9e-16
Identity = 44/60 (73.33%), Postives = 50/60 (83.33%), Query Frame = 1


Query: 5   FLLKALDRGVALLKVVKLATGHNAIDMVGTILLNILRGDIPRLSRCTALITDEDGPNPRC 64
           F  +ALDRG ALLKV KLATGHNA D+  T+LLNILRGDI RLSRCT+++T EDGP PRC
Sbjct: 158 FRRQALDRGAALLKVDKLATGHNADDIAETVLLNILRGDIARLSRCTSIVTGEDGPIPRC 217

The following BLAST results are available for this feature:

BLAST of MELO3C003855 vs. Swiss-Prot


Match Name	E-value	Identity	Description
CTU1_ARATH	3.6e-17	71.67	Cytoplasmic tRNA 2-thiolation protein 1 OS=Arabidopsis thaliana GN=NCS6 PE=2 SV=...	[more]
CTU1_ORYSJ	1.8e-16	70.00	Cytoplasmic tRNA 2-thiolation protein 1 OS=Oryza sativa subsp. japonica GN=NCS6 ...	[more]
CTU1_CHLRE	1.2e-12	59.32	Cytoplasmic tRNA 2-thiolation protein 1 OS=Chlamydomonas reinhardtii GN=NCS6 PE=...	[more]
CTU1_DICDI	2.7e-12	55.93	Cytoplasmic tRNA 2-thiolation protein 1 OS=Dictyostelium discoideum GN=ctu1 PE=2...	[more]
CTU1_XENLA	3.5e-12	55.00	Cytoplasmic tRNA 2-thiolation protein 1 OS=Xenopus laevis GN=ctu1 PE=2 SV=1	[more]

BLAST of MELO3C003855 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LKF0_CUCSA	7.3e-17	78.33	Cytoplasmic tRNA 2-thiolation protein 1 OS=Cucumis sativus GN=NCS6 PE=3 SV=1	[more]
A0A0D3D124_BRAOL	9.6e-17	72.58	Uncharacterized protein OS=Brassica oleracea var. oleracea PE=4 SV=1	[more]
A0A103XR73_CYNCS	4.8e-16	75.00	Rossmann-like alpha/beta/alpha sandwich fold (Fragment) OS=Cynara cardunculus va...	[more]
M5XB75_PRUPE	6.2e-16	73.33	Cytoplasmic tRNA 2-thiolation protein 1 OS=Prunus persica GN=NCS6 PE=3 SV=1	[more]
E0CQT4_VITVI	8.1e-16	73.33	Cytoplasmic tRNA 2-thiolation protein 1 OS=Vitis vinifera GN=NCS6 PE=3 SV=1	[more]

BLAST of MELO3C003855 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G44270.2	2.0e-18	71.67	repressor of lrx1	[more]
AT1G76170.1	5.9e-18	70.00	2-thiocytidine tRNA biosynthesis protein, TtcA	[more]

BLAST of MELO3C003855 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659093684\|ref\|XP_008447661.1\|	5.0e-19	91.07	PREDICTED: cytoplasmic tRNA 2-thiolation protein 1-like [Cucumis melo]	[more]
gi\|778670437\|ref\|XP_011649473.1\|	1.1e-16	78.33	PREDICTED: cytoplasmic tRNA 2-thiolation protein 1 [Cucumis sativus]	[more]
gi\|659089053\|ref\|XP_008445303.1\|	5.2e-16	76.67	PREDICTED: cytoplasmic tRNA 2-thiolation protein 1 [Cucumis melo]	[more]
gi\|976908178\|gb\|KVH95408.1\|	6.8e-16	75.00	Rossmann-like alpha/beta/alpha sandwich fold, partial [Cynara cardunculus var. s...	[more]
gi\|596130726\|ref\|XP_007222158.1\|	8.9e-16	73.33	hypothetical protein PRUPE_ppa007715mg [Prunus persica]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032447	protein urmylation
biological_process	GO:0034227	tRNA thio-modification
biological_process	GO:0002098	tRNA wobble uridine modification
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0016779	nucleotidyltransferase activity
molecular_function	GO:0000049	tRNA binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MELO3C003855T1	MELO3C003855T1	mRNA

InterPro

Analysis Name: InterPro Annotations of melon
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR11807	ATPASES OF THE PP SUPERFAMILY-RELATED	coord: 9..64 score: 3.5
None	No IPR available	PANTHER	PTHR11807:SF12	CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 1	coord: 9..64 score: 3.5
None	No IPR available	unknown	SSF52402	Adenine nucleotide alpha hydrolases-like	coord: 15..55 score: 9.2

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
MELO3C003855	Cucurbita pepo (Zucchini)	cpemeB516
MELO3C003855	Cucurbita pepo (Zucchini)	cpemeB726
MELO3C003855	Bottle gourd (USVL1VR-Ls)	lsimeB142
MELO3C003855	Bottle gourd (USVL1VR-Ls)	lsimeB311
MELO3C003855	Cucumber (Gy14) v2	cgybmeB185
MELO3C003855	Cucumber (Gy14) v2	cgybmeB427
MELO3C003855	Silver-seed gourd	carmeB0106
MELO3C003855	Silver-seed gourd	carmeB0928
MELO3C003855	Cucumber (Chinese Long) v3	cucmeB219
MELO3C003855	Cucumber (Chinese Long) v3	cucmeB498
MELO3C003855	Watermelon (97103) v2	mewmbB315
MELO3C003855	Watermelon (97103) v2	mewmbB332
MELO3C003855	Wax gourd	mewgoB384
MELO3C003855	Wax gourd	mewgoB425
MELO3C003855	Melon (DHL92) v3.5.1	memeB145
MELO3C003855	Cucumber (Gy14) v1	cgymeB202
MELO3C003855	Cucumber (Gy14) v1	cgymeB559
MELO3C003855	Cucurbita maxima (Rimu)	cmameB032
MELO3C003855	Cucurbita maxima (Rimu)	cmameB424
MELO3C003855	Cucurbita moschata (Rifu)	cmomeB021
MELO3C003855	Cucurbita moschata (Rifu)	cmomeB414
MELO3C003855	Wild cucumber (PI 183967)	cpimeB215
MELO3C003855	Wild cucumber (PI 183967)	cpimeB488
MELO3C003855	Cucumber (Chinese Long) v2	cumeB222
MELO3C003855	Cucumber (Chinese Long) v2	cumeB485
MELO3C003855	Watermelon (Charleston Gray)	mewcgB326
MELO3C003855	Watermelon (Charleston Gray)	mewcgB331
MELO3C003855	Watermelon (97103) v1	mewmB368
MELO3C003855	Watermelon (97103) v1	mewmB378