MELO3C003328 (gene) Melon (DHL92) v3.5.1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	MELO3C003328
Type	gene
Organism	Cucumis melo (Melon (DHL92) v3.5.1)
Description	tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG
Location	chr4 : 338336 .. 339710 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACTATGGCTTCTTCTTCATCGTCTGTGTTCCTCAAATCTCCGATTTCGCCATCTCCAGCTCTCCATTTCCATGGGGGAGAATTTCCCAAGAGCCAACCGTGGATTCGGGGTCGACCCAGGACAAAAAGTGTGGTCAAAGCTCAAACAGAGGTCAGGAGGGAAGACATAGTTATAATTGGAGGAGGGATTGCTGGTCTTGCTACTGCTCTATCTCTTCACAGGTAGGATCTAATTTTACTAAAATCTATATTGCTTAGAGTTTAAGTGCAGATGGTTTTATTCTTGAGGCTTTTAAATTTCAGGCTTGGAGTTAGGTCATTGGTGCTTGAGCAAGCTGAATCACTTAGAACTGGTGGAACCTCATTGACCCTTTTCAAAAATGGATGGCGTGTGCTGGATGCAATTGGGGTTGGAAATGTTCTCAGGACCCAATTTCTTGAACTACAAGGGTATTTTCTAGTTTTTCTTGGTGCAGATTTGTTGTGTTTAGTGCTTTATACATCTGTTGGTATGGAGATTAGCACATAGTTCATTAAGTGACCAAAGCAATAATTTCAATTCAAATCATCTGGCTTTGATAAATGATACTATATTAAATCATCGTTAAGACTCTAATGTTTGAAACTGATAGATCATAGTTCGTTGTACATTTAACTAAGACTGGACTGGGTTTTGTTTTTATTTTTTTCTTTTTTAATACGAGAACAACAACATTCATATAAATGCAAACAGAAAAAACAAGCCCACGAAAGACGGGAGTTTTCCAAGAAAAAGGACTCCAACTATGCGAAACAAGCCTAGAGAATAGTTTCAAAAGGTCTTTGAAATCAATTCCTACACAGGAACTTGAAAGTGAACGATGGACGAAATCTCAAAAACACCTTGTTATTGGTTAATTGTTGATTTCGTAACAAATTGTTTATATACCAGCATTGCAGGATGGTAGTTAAGTCAGAAGAAGGAAAGCAGCTGCGCTCCTTCACGTTTAAGGACGAAGATGAGAGGTACTCAGTTTTTCTATCTTTCCTGTTTGTGGAACTGAATCAGAAATTGGGTATATGCTAAACATCTTTTCCCTCCTAAGAAACGGTTTCATCATCTTAGTCTTGCTTTCACCTACGATTGTGAAGGGAATTTGAGACCGCTTTAACTCTATTAACCATTTTTCGACAGCCAAGAGGTGCGTGCAGTGGAGAGGAGAACACTGCTGGAAACTCTAGCCTCTCAGCTTCCAGCAGGAACGATTCAGTTTTCCTCTAAGCTTGAAGCCATTCAGAGAACTCATCAAGATGAAGTCAAGTTGGAACTTGTGGATGGGACACAGTTGACTGCAAAGGTAATTCAAACTTCAAATTTCAAGAGAATTGACTAA

mRNA sequence

ATGACTATGGCTTCTTCTTCATCGTCTGTGTTCCTCAAATCTCCGATTTCGCCATCTCCAGCTCTCCATTTCCATGGGGGAGAATTTCCCAAGAGCCAACCGTGGATTCGGGGTCGACCCAGGACAAAAAGTGTGGTCAAAGCTCAAACAGAGGTCAGGAGGGAAGACATAGTTATAATTGGAGGAGGGATTGCTGGTCTTGCTACTGCTCTATCTCTTCACAGGCTTGGAGTTAGGTCATTGGTGCTTGAGCAAGCTGAATCACTTAGAACTGGTGGAACCTCATTGACCCTTTTCAAAAATGGATGGCGTGTGCTGGATGCAATTGGGGTTGGAAATGTTCTCAGGACCCAATTTCTTGAACTACAAGGGATGGTAGTTAAGTCAGAAGAAGGAAAGCAGCTGCGCTCCTTCACGTTTAAGGACGAAGATGAGAGCCAAGAGGTGCGTGCAGTGGAGAGGAGAACACTGCTGGAAACTCTAGCCTCTCAGCTTCCAGCAGGAACGATTCAGTTTTCCTCTAAGCTTGAAGCCATTCAGAGAACTCATCAAGATGAAGTCAAGTTGGAACTTGTGGATGGGACACAGTTGACTGCAAAGGTAATTCAAACTTCAAATTTCAAGAGAATTGACTAA

Coding sequence (CDS)

ATGACTATGGCTTCTTCTTCATCGTCTGTGTTCCTCAAATCTCCGATTTCGCCATCTCCAGCTCTCCATTTCCATGGGGGAGAATTTCCCAAGAGCCAACCGTGGATTCGGGGTCGACCCAGGACAAAAAGTGTGGTCAAAGCTCAAACAGAGGTCAGGAGGGAAGACATAGTTATAATTGGAGGAGGGATTGCTGGTCTTGCTACTGCTCTATCTCTTCACAGGCTTGGAGTTAGGTCATTGGTGCTTGAGCAAGCTGAATCACTTAGAACTGGTGGAACCTCATTGACCCTTTTCAAAAATGGATGGCGTGTGCTGGATGCAATTGGGGTTGGAAATGTTCTCAGGACCCAATTTCTTGAACTACAAGGGATGGTAGTTAAGTCAGAAGAAGGAAAGCAGCTGCGCTCCTTCACGTTTAAGGACGAAGATGAGAGCCAAGAGGTGCGTGCAGTGGAGAGGAGAACACTGCTGGAAACTCTAGCCTCTCAGCTTCCAGCAGGAACGATTCAGTTTTCCTCTAAGCTTGAAGCCATTCAGAGAACTCATCAAGATGAAGTCAAGTTGGAACTTGTGGATGGGACACAGTTGACTGCAAAGGTAATTCAAACTTCAAATTTCAAGAGAATTGACTAA

Protein sequence

MTMASSSSSVFLKSPISPSPALHFHGGEFPKSQPWIRGRPRTKSVVKAQTEVRREDIVIIGGGIAGLATALSLHRLGVRSLVLEQAESLRTGGTSLTLFKNGWRVLDAIGVGNVLRTQFLELQGMVVKSEEGKQLRSFTFKDEDESQEVRAVERRTLLETLASQLPAGTIQFSSKLEAIQRTHQDEVKLELVDGTQLTAKVIQTSNFKRID*

Homology

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BLAST of MELO3C003328 vs. Swiss-Prot
Match: HPXO_ACIAD (FAD-dependent urate hydroxylase OS=Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1) GN=hpxO PE=1 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 6.9e-09
Identity = 43/154 (27.92%), Postives = 74/154 (48.05%), Query Frame = 1


Query: 56  DIVIIGGGIAGLATALSLHRLGVRSLVLEQAESLRTGGTSLTLFKNGWRVLDAIGVGNVL 115
           ++VIIG G+ GL T ++L + G +  + EQAE +   G +++L+ NG + L+ +G    L
Sbjct: 2   NVVIIGAGMGGLTTGIALKKFGHQVTIFEQAEQILPVGAAISLWSNGVKCLNYLG----L 61

Query: 116 RTQFLELQGMV-----VKSEEGKQLRSFTFKD--EDESQEVRAVERRTLLETLASQLPAG 175
             Q  +L G +     V    G  +  F+ +   E+  Q    V R  L   L  +    
Sbjct: 62  NEQIAKLGGQMDNLAYVDGLTGDVMTEFSLQPLIEEVGQRPYPVSRAELQNMLMDEFGRE 121

Query: 176 TIQFSSKLEAIQRTHQDEVKLELVDGTQLTAKVI 203
            I    ++ A+Q+   D+V++E  DG+ + A V+
Sbjct: 122 DIHLGKRMVALQQ-KDDQVEIEFADGSSILADVL 150

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BLAST of MELO3C003328 vs. Swiss-Prot
Match: 3HBH1_PSEAC (3-hydroxybenzoate 6-hydroxylase 1 OS=Pseudomonas alcaligenes GN=xlnD PE=1 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 2.9e-07
Identity = 29/61 (47.54%), Postives = 37/61 (60.66%), Query Frame = 1


Query: 56  DIVIIGGGIAGLATALSLHRLGVRSLVLEQAESLRTGGTSLTLFKNGWRVLDAIGVGNVL 115
           +I+I G GI GL+ AL L R G+RS+VLE+A  L   G  + L  N +  LDA+G+G V 
Sbjct: 4   NILIAGAGIGGLSAALGLARKGMRSIVLEKAPELGEIGAGIQLAPNAYHALDALGIGEVA 63

Query: 116 R 117
           R
Sbjct: 64  R 64

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BLAST of MELO3C003328 vs. Swiss-Prot
Match: BIK2_GIBF5 (FAD-dependent monooxygenase bik2 OS=Gibberella fujikuroi (strain CBS 195.34 / IMI 58289 / NRRL A-6831) GN=bik2 PE=1 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 5.0e-07
Identity = 39/152 (25.66%), Postives = 72/152 (47.37%), Query Frame = 1


Query: 56  DIVIIGGGIAGLATALSLHRLGVRSLVLEQAESLRTG-GTSLTLFKNGWRVLDAIGVGNV 115
           +++I GGGIAG+  AL   +L +   +LE  ++L +  G  + L  NG R+LD +G+   
Sbjct: 9   EVIIAGGGIAGVTLALMFEKLDISYFLLEGRDTLESDRGAGIGLQPNGLRILDQLGLVED 68

Query: 116 LRTQFLELQGMVVKSEEGKQLRSFTFKDEDESQ---EVRAVERRTLLETLASQLP-AGTI 175
           +    + L+       EG  +       +   +    V  +ERR LL  +   +     +
Sbjct: 69  IEEATIPLEKWFSYDSEGNLMNDSDAMGQYRDKIGYPVAFIERRKLLPIMVRHIQRTECV 128

Query: 176 QFSSKLEAIQRTHQDEVKLELVDGTQLTAKVI 203
           + S+++ +I+ + +D V +   DG  LTA ++
Sbjct: 129 KTSARVASIEES-EDHVTVTTTDGLSLTADIV 159

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BLAST of MELO3C003328 vs. Swiss-Prot
Match: 3HBH_KLEOX (3-hydroxybenzoate 6-hydroxylase OS=Klebsiella oxytoca GN=mhbM PE=1 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 5.0e-07
Identity = 27/59 (45.76%), Postives = 37/59 (62.71%), Query Frame = 1


Query: 58  VIIGGGIAGLATALSLHRLGVRSLVLEQAESLRTGGTSLTLFKNGWRVLDAIGVGNVLR 117
           +I+GGGI G ATALSL R G++ ++LE+A  +   G  + L  N +  LD++GVG V R
Sbjct: 8   IIVGGGIGGAATALSLARQGIKVMLLEKAHEIGEIGAGIQLGPNAFSALDSLGVGEVAR 66

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BLAST of MELO3C003328 vs. Swiss-Prot
Match: ATMM_ASPFL (FAD-dependent monooxygenase atmM OS=Aspergillus flavus GN=atmM PE=2 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 1.9e-06
Identity = 40/153 (26.14%), Postives = 70/153 (45.75%), Query Frame = 1


Query: 54  REDIVIIGGGIAGLATALSLHRLGVRSLVLEQAESLRTG-GTSLTLFKNGWRVLDAIGVG 113
           R  ++I+GG +AGL  A  L R G+  +VLE+   L    G S+ +  NG R+LD +G+ 
Sbjct: 6   RFKVIIVGGSVAGLTLAHCLQRAGIDHVVLEKNSDLSPQVGASIGIIPNGGRILDQLGLF 65

Query: 114 NVLRTQFLELQGMVVKSEEGKQLRSFTFKDEDE--SQEVRAVERRTLLETLASQLP-AGT 173
           + +      L    +   +G   R+   K  DE     +  ++R+  LE L +  P    
Sbjct: 66  DAVERMTYPLSIATITYPDGYSFRNNYPKTVDERFGYPIAFLDRQKFLEILHTSYPDPSN 125

Query: 174 IQFSSKLEAIQRTHQDEVKLELVDGTQLTAKVI 203
           I  + ++  I+R H   +++    G + T  ++
Sbjct: 126 IHTNCRVTHIRR-HDSHMEVVTSSGQEYTGDLV 157

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BLAST of MELO3C003328 vs. TrEMBL
Match: A0A0A0LEK6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G912970 PE=4 SV=1)

HSP 1 Score: 373.2 bits (957), Expect = 2.0e-100
Identity = 193/202 (95.54%), Postives = 197/202 (97.52%), Query Frame = 1


Query: 1   MTMASSSSSVFLKSPISPSPALHFHGGEFPKSQPWIRGRPRTKSVVKAQTEVRREDIVII 60
           MTMASSSSSVFLKSP SPSPALHF GGEFPKSQPWIRGRPRTK VVKAQTEVRREDIVII
Sbjct: 1   MTMASSSSSVFLKSPTSPSPALHFRGGEFPKSQPWIRGRPRTKIVVKAQTEVRREDIVII 60

Query: 61  GGGIAGLATALSLHRLGVRSLVLEQAESLRTGGTSLTLFKNGWRVLDAIGVGNVLRTQFL 120
           GGGIAGLATALSLHRLGVRSLVLEQAESLRTGGTSLTLFKNGWRVLDAIGVGNVLRTQFL
Sbjct: 61  GGGIAGLATALSLHRLGVRSLVLEQAESLRTGGTSLTLFKNGWRVLDAIGVGNVLRTQFL 120

Query: 121 ELQGMVVKSEEGKQLRSFTFKDEDESQEVRAVERRTLLETLASQLPAGTIQFSSKLEAIQ 180
           E+QGMVVKSEEGKQLRSFTFKDEDESQEVRAVERRTLLETLAS LPAGTIQFSSKLEAIQ
Sbjct: 121 EVQGMVVKSEEGKQLRSFTFKDEDESQEVRAVERRTLLETLASHLPAGTIQFSSKLEAIQ 180

Query: 181 RTHQDEVKLELVDGTQLTAKVI 203
           RTHQ+EVKLELVDGTQL AK++
Sbjct: 181 RTHQNEVKLELVDGTQLIAKIV 202

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BLAST of MELO3C003328 vs. TrEMBL
Match: D7TG98_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_00s0533g00020 PE=4 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 1.5e-63
Identity = 135/209 (64.59%), Postives = 162/209 (77.51%), Query Frame = 1


Query: 3   MASSSSSVFLKSPISPSPAL-----HFHGGEFPKSQPW----IRGRPRTKSVVKAQTEVR 62
           MA S +S+ L SP+SP  +      H     FP+SQ +     R +P + S+V+AQ  VR
Sbjct: 1   MAKSLASLLLNSPLSPPSSFSQSLHHLQAKTFPQSQSYSGVGTRTKPISASMVEAQPPVR 60

Query: 63  REDIVIIGGGIAGLATALSLHRLGVRSLVLEQAESLRTGGTSLTLFKNGWRVLDAIGVGN 122
           +EDI+I+G GIAGLATA+SLHRLGV SLVLEQAESLRTGGTSLTLFKNGW VLDA+GVGN
Sbjct: 61  KEDIIIVGAGIAGLATAVSLHRLGVGSLVLEQAESLRTGGTSLTLFKNGWGVLDAMGVGN 120

Query: 123 VLRTQFLELQGMVVKSEEGKQLRSFTFKDEDESQEVRAVERRTLLETLASQLPAGTIQFS 182
            LR+QFLE+QGMVVKSE+G++LRSF FKDEDESQEVRAVERR LLETLA+QLP  +I FS
Sbjct: 121 DLRSQFLEIQGMVVKSEDGRELRSFRFKDEDESQEVRAVERRILLETLANQLPTDSIHFS 180

Query: 183 SKLEAIQRTHQDEVKLELVDGTQLTAKVI 203
           SKL  I+R    E  LEL DGT+L+ K++
Sbjct: 181 SKLAKIERIETGETLLELEDGTRLSGKIV 209

[Close]

BLAST of MELO3C003328 vs. TrEMBL
Match: I3S124_LOTJA (Uncharacterized protein OS=Lotus japonicus PE=2 SV=1)

HSP 1 Score: 243.4 bits (620), Expect = 2.4e-61
Identity = 131/200 (65.50%), Postives = 154/200 (77.00%), Query Frame = 1


Query: 2   TMASSSSSVFLKSPISPSPALHFHGGEFPKSQPWIRGRPRTKSVVKAQTEVRREDIVIIG 61
           T  ++SS VFLKSP S       H   F       R R     ++KAQ+EVR+E +VI+G
Sbjct: 4   TTTTTSSFVFLKSPAS---TFLMHNDHFRYGNGARRRRSSKPIIIKAQSEVRKEHVVIVG 63

Query: 62  GGIAGLATALSLHRLGVRSLVLEQAESLRTGGTSLTLFKNGWRVLDAIGVGNVLRTQFLE 121
           GGIAGLATALSLHRLGVRSLVLEQAESLRTGGTSLTL KNGWRVLDAIGV N LR QFLE
Sbjct: 64  GGIAGLATALSLHRLGVRSLVLEQAESLRTGGTSLTLMKNGWRVLDAIGVANELRPQFLE 123

Query: 122 LQGMVVKSEEGKQLRSFTFKDEDESQEVRAVERRTLLETLASQLPAGTIQFSSKLEAIQR 181
           +QGMVVK+E+G++LRSF FK+ED+SQEVRAVERR LLETLA  LP  TIQ+SS+L +I+ 
Sbjct: 124 IQGMVVKTEDGRELRSFNFKEEDQSQEVRAVERRVLLETLAGHLPQDTIQYSSRLASIEA 183

Query: 182 THQDEVKLELVDGTQLTAKV 202
           T   +  LELVDG++L AK+
Sbjct: 184 TPNGDTLLELVDGSKLLAKM 200

[Close]

BLAST of MELO3C003328 vs. TrEMBL
Match: M5W9D2_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa005571mg PE=4 SV=1)

HSP 1 Score: 243.0 bits (619), Expect = 3.2e-61
Identity = 137/209 (65.55%), Postives = 163/209 (77.99%), Query Frame = 1


Query: 3   MASSSSSVFLKSPISPSPALHFHGGEFPKSQP-WIRGRPRTK------SVVKAQT--EVR 62
           MA+ S S+  ++P+  SP  H      P++Q  W +   RT+      S ++AQ+    R
Sbjct: 1   MATVSMSLVFQNPLL-SP-YHSKTRPLPRNQSNWFQAPTRTRTKPTSFSSIRAQSGAAAR 60

Query: 63  REDIVIIGGGIAGLATALSLHRLGVRSLVLEQAESLRTGGTSLTLFKNGWRVLDAIGVGN 122
           +EDIVI+G GIAGLATALSLHRLGV SLVLEQAESLRTGGTSLTLFKNGWRVLDA+GVGN
Sbjct: 61  KEDIVIVGAGIAGLATALSLHRLGVGSLVLEQAESLRTGGTSLTLFKNGWRVLDAMGVGN 120

Query: 123 VLRTQFLELQGMVVKSEEGKQLRSFTFKDEDESQEVRAVERRTLLETLASQLPAGTIQFS 182
            LRTQFLE+QGMVVK+E+G++LRSF FKDEDESQEVR VER  LLETLA+QLPAG ++FS
Sbjct: 121 DLRTQFLEIQGMVVKTEDGRELRSFKFKDEDESQEVRPVERGILLETLANQLPAGAVRFS 180

Query: 183 SKLEAIQRTHQDEVKLELVDGTQLTAKVI 203
           SKL  IQ+T   E  LELVDGTQL+AKV+
Sbjct: 181 SKLAKIQKTENGETLLELVDGTQLSAKVV 207

[Close]

BLAST of MELO3C003328 vs. TrEMBL
Match: K4C6R8_SOLLC (Uncharacterized protein OS=Solanum lycopersicum PE=4 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 6.6e-59
Identity = 126/202 (62.38%), Postives = 158/202 (78.22%), Query Frame = 1


Query: 5   SSSSSVFLKSPISPS----PALHFHGGEFPKSQPWIRGRPRTKSVVKAQTEVRREDIVII 64
           SSS+ + +KS IS S    P+L+F  GE       +R RP + S++ A+ + R+EDIVI+
Sbjct: 4   SSSTLLLVKSHISLSSFSNPSLYFRRGETR-----LRVRPISLSIINARADERKEDIVIV 63

Query: 65  GGGIAGLATALSLHRLGVRSLVLEQAESLRTGGTSLTLFKNGWRVLDAIGVGNVLRTQFL 124
           G GIAGLATA+SL RLG+R+LVLEQ ESLRTGGTSLTLFKNGW+ LDAIGVGN LR+QFL
Sbjct: 64  GAGIAGLATAVSLQRLGIRTLVLEQGESLRTGGTSLTLFKNGWKALDAIGVGNDLRSQFL 123

Query: 125 ELQGMVVKSEEGKQLRSFTFKDEDESQEVRAVERRTLLETLASQLPAGTIQFSSKLEAIQ 184
           E+QGM +KSE+G++LRSF FKDEDESQEVRAVERR LLETLAS+LP   I FSSKL  I+
Sbjct: 124 EIQGMAIKSEDGRELRSFRFKDEDESQEVRAVERRVLLETLASRLPPDAISFSSKLANIE 183

Query: 185 RTHQDEVKLELVDGTQLTAKVI 203
           R+   E  L+L DG +++AK++
Sbjct: 184 RSENGETLLKLEDGIRISAKIL 200

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BLAST of MELO3C003328 vs. TAIR10
Match: AT2G35660.1 (AT2G35660.1 FAD/NAD(P)-binding oxidoreductase family protein)

HSP 1 Score: 217.6 bits (553), Expect = 7.3e-57
Identity = 113/168 (67.26%), Postives = 135/168 (80.36%), Query Frame = 1


Query: 37  RGRPRTKSVVKAQTEV--RREDIVIIGGGIAGLATALSLHRLGVRSLVLEQAESLRTGGT 96
           R +P   ++  AQT    + E +VI+G GI GLATA+SLHRLG+RS+VLEQAESLRTGGT
Sbjct: 25  RSKPVCLALTNAQTNGGDQEEKVVIVGAGIGGLATAVSLHRLGIRSVVLEQAESLRTGGT 84

Query: 97  SLTLFKNGWRVLDAIGVGNVLRTQFLELQGMVVKSEEGKQLRSFTFKDEDESQEVRAVER 156
           SLTLFKNGWRVLDAI VG  LRTQFLE++GMVVK E+G++LRSF FKD+D+SQEVRAVER
Sbjct: 85  SLTLFKNGWRVLDAISVGPQLRTQFLEIEGMVVKKEDGRELRSFKFKDDDQSQEVRAVER 144

Query: 157 RTLLETLASQLPAGTIQFSSKLEAIQRTHQDEVKLELVDGTQLTAKVI 203
           R LLETLASQLP  TI+FSSKLE+IQ     +  L+L DGT+L A+++
Sbjct: 145 RVLLETLASQLPPQTIRFSSKLESIQSNANGDTLLQLGDGTRLLAQIV 192

[Close]

BLAST of MELO3C003328 vs. TAIR10
Match: AT2G29720.1 (AT2G29720.1 FAD/NAD(P)-binding oxidoreductase family protein)

HSP 1 Score: 207.6 bits (527), Expect = 7.5e-54
Identity = 109/178 (61.24%), Postives = 131/178 (73.60%), Query Frame = 1


Query: 31  KSQPWIRGRPRTKSVVKAQTEV------RREDIVIIGGGIAGLATALSLHRLGVRSLVLE 90
           +S+ W   R   KSV    T        R E +VI+GGGI GLATA++LHRLG+RS+VLE
Sbjct: 13  RSKRWFPVRTGLKSVCLTTTRAETSGVDREEKVVIVGGGIGGLATAVALHRLGIRSVVLE 72

Query: 91  QAESLRTGGTSLTLFKNGWRVLDAIGVGNVLRTQFLELQGMVVKSEEGKQLRSFTFKDED 150
           QAESLRTGG SLTL KNGWRVLDAI +G  LR QFLE+QG+V+K E+G++LRSF FKD D
Sbjct: 73  QAESLRTGGASLTLSKNGWRVLDAISIGPQLRKQFLEMQGVVLKKEDGRELRSFKFKDND 132

Query: 151 ESQEVRAVERRTLLETLASQLPAGTIQFSSKLEAIQRTHQDEVKLELVDGTQLTAKVI 203
           +SQEVR VERR LLETLASQLP  TI+FSSKLE+IQ     +  L+L DGT+  A ++
Sbjct: 133 QSQEVRGVERRVLLETLASQLPPQTIRFSSKLESIQSNANGDTLLQLKDGTRFLANIV 190

[Close]

BLAST of MELO3C003328 vs. TAIR10
Match: AT5G05320.1 (AT5G05320.1 FAD/NAD(P)-binding oxidoreductase family protein)

HSP 1 Score: 121.3 bits (303), Expect = 7.1e-28
Identity = 66/148 (44.59%), Postives = 93/148 (62.84%), Query Frame = 1


Query: 55  EDIVIIGGGIAGLATALSLHRLGVRSLVLEQAESLRTGGTSLTLFKNGWRVLDAIGVGNV 114
           +DI+I+G GI+GLATAL LHRLG+RS+VLE +E LR  G +L+L+ N W+ ++A+G+   
Sbjct: 7   QDIIIVGAGISGLATALGLHRLGIRSIVLESSEQLRATGFALSLYFNAWKAMEALGISQH 66

Query: 115 LRTQFLELQGMVVKSEEGKQLRSFTFKDEDESQEVRAVERRTLLETLASQLPAGTIQFSS 174
           +R+     QG VV+              E E  EVR V+R+ LL+ LA +LP GTI+FSS
Sbjct: 67  IRSLGDRFQGWVVRPISAGDPPKEMLFPESEEYEVRCVQRKLLLDALAGELPQGTIRFSS 126

Query: 175 KLEAIQRTHQDEVKLELVDGTQLTAKVI 203
           KL  I+ +   ++ + L DGT L  KV+
Sbjct: 127 KLVHIELSGHYKM-VHLSDGTILKTKVL 153

[Close]

BLAST of MELO3C003328 vs. TAIR10
Match: AT4G38540.1 (AT4G38540.1 FAD/NAD(P)-binding oxidoreductase family protein)

HSP 1 Score: 111.7 bits (278), Expect = 5.6e-25
Identity = 63/147 (42.86%), Postives = 91/147 (61.90%), Query Frame = 1


Query: 56  DIVIIGGGIAGLATALSLHRLGVRSLVLEQAESLRTGGTSLTLFKNGWRVLDAIGVGNVL 115
           DI+I+G GI+GL+TA+ LHRLG+RS+VLE +E+LR  G + T + N W+ ++A+GV   +
Sbjct: 8   DIIIVGAGISGLSTAVGLHRLGIRSMVLESSETLRATGFAFTTWFNAWKAMEALGVSQHI 67

Query: 116 RTQFLELQGMVVKSEEGKQLRSFTFKDEDESQEVRAVERRTLLETLASQLPAGTIQFSSK 175
           R+    L+G VV +       +     E E  E R V+R+ LLE LA +LP  TI+FSSK
Sbjct: 68  RSLHDRLEGWVVGTISAGTPPTEMLFPESEEYESRCVQRKLLLEALAGELPEETIRFSSK 127

Query: 176 LEAIQRTHQDEVKLELVDGTQLTAKVI 203
           +  I+ +   + K+ L DGT L  KV+
Sbjct: 128 VVHIELSGCYK-KVHLSDGTILKTKVL 153

[Close]

BLAST of MELO3C003328 vs. TAIR10
Match: AT4G15760.1 (AT4G15760.1 monooxygenase 1)

HSP 1 Score: 105.5 bits (262), Expect = 4.0e-23
Identity = 65/157 (41.40%), Postives = 93/157 (59.24%), Query Frame = 1


Query: 51  EVRREDIVIIGGGIAGLATALSLHRLGVRSLVLEQAESLRTGGTSLTLFKNGWRVLDAIG 110
           E+    IVI+GGGIAGLAT+++LHR G++S+VLE+AE +R+ G  +    NGWR LD +G
Sbjct: 25  EMEEIGIVIVGGGIAGLATSIALHRKGIKSVVLERAEKVRSEGAGIGTLSNGWRALDQLG 84

Query: 111 VGNVLR-TQFLELQGMVVKSEEGKQLRSFTFKDEDESQEVRAVERRTLLETLASQLPAGT 170
           VG+ LR    L  +   +  E GK+ R F     DE+   R ++R  L+E L+  LP GT
Sbjct: 85  VGDRLRLNSSLIHKARTMLIENGKK-REFVSNIVDEA---RCIKRNDLVEALSDALPKGT 144

Query: 171 IQFSSKLEAIQRTHQDEVKL----ELVDGTQLTAKVI 203
           I+F S + +I+   QD+  L     L +G  + AKV+
Sbjct: 145 IRFGSHIVSIE---QDKTTLFPVVHLANGNSIKAKVL 174

[Close]

BLAST of MELO3C003328 vs. NCBI nr
Match: gi|659131847|ref|XP_008465886.1| (PREDICTED: kynurenine 3-monooxygenase-like, partial [Cucumis melo])

HSP 1 Score: 387.9 bits (995), Expect = 1.1e-104
Identity = 200/202 (99.01%), Postives = 202/202 (100.00%), Query Frame = 1


Query: 1   MTMASSSSSVFLKSPISPSPALHFHGGEFPKSQPWIRGRPRTKSVVKAQTEVRREDIVII 60
           MTMASSSSSVFLKSPISPSPALHFHGGEFPKSQPWIRGRPRTKSVVKAQTEVRREDIVII
Sbjct: 1   MTMASSSSSVFLKSPISPSPALHFHGGEFPKSQPWIRGRPRTKSVVKAQTEVRREDIVII 60

Query: 61  GGGIAGLATALSLHRLGVRSLVLEQAESLRTGGTSLTLFKNGWRVLDAIGVGNVLRTQFL 120
           GGGIAGLATALSLHRLGVRSLVLEQAESLRTGGTSLTLFKNGWRVLDAIGVGNVLRTQFL
Sbjct: 61  GGGIAGLATALSLHRLGVRSLVLEQAESLRTGGTSLTLFKNGWRVLDAIGVGNVLRTQFL 120

Query: 121 ELQGMVVKSEEGKQLRSFTFKDEDESQEVRAVERRTLLETLASQLPAGTIQFSSKLEAIQ 180
           ELQGMVVKSEEGKQLRSFTFKDEDESQEVRAVERRTLLETLASQLPAGTIQFSSKLEAIQ
Sbjct: 121 ELQGMVVKSEEGKQLRSFTFKDEDESQEVRAVERRTLLETLASQLPAGTIQFSSKLEAIQ 180

Query: 181 RTHQDEVKLELVDGTQLTAKVI 203
           RTHQDEVKLELVDGTQLTAK++
Sbjct: 181 RTHQDEVKLELVDGTQLTAKIV 202

[Close]

BLAST of MELO3C003328 vs. NCBI nr
Match: gi|778688179|ref|XP_004148358.2| (PREDICTED: zeaxanthin epoxidase, chloroplastic isoform X1 [Cucumis sativus])

HSP 1 Score: 373.2 bits (957), Expect = 2.9e-100
Identity = 193/202 (95.54%), Postives = 197/202 (97.52%), Query Frame = 1


Query: 1   MTMASSSSSVFLKSPISPSPALHFHGGEFPKSQPWIRGRPRTKSVVKAQTEVRREDIVII 60
           MTMASSSSSVFLKSP SPSPALHF GGEFPKSQPWIRGRPRTK VVKAQTEVRREDIVII
Sbjct: 1   MTMASSSSSVFLKSPTSPSPALHFRGGEFPKSQPWIRGRPRTKIVVKAQTEVRREDIVII 60

Query: 61  GGGIAGLATALSLHRLGVRSLVLEQAESLRTGGTSLTLFKNGWRVLDAIGVGNVLRTQFL 120
           GGGIAGLATALSLHRLGVRSLVLEQAESLRTGGTSLTLFKNGWRVLDAIGVGNVLRTQFL
Sbjct: 61  GGGIAGLATALSLHRLGVRSLVLEQAESLRTGGTSLTLFKNGWRVLDAIGVGNVLRTQFL 120

Query: 121 ELQGMVVKSEEGKQLRSFTFKDEDESQEVRAVERRTLLETLASQLPAGTIQFSSKLEAIQ 180
           E+QGMVVKSEEGKQLRSFTFKDEDESQEVRAVERRTLLETLAS LPAGTIQFSSKLEAIQ
Sbjct: 121 EVQGMVVKSEEGKQLRSFTFKDEDESQEVRAVERRTLLETLASHLPAGTIQFSSKLEAIQ 180

Query: 181 RTHQDEVKLELVDGTQLTAKVI 203
           RTHQ+EVKLELVDGTQL AK++
Sbjct: 181 RTHQNEVKLELVDGTQLIAKIV 202

[Close]

BLAST of MELO3C003328 vs. NCBI nr
Match: gi|659131845|ref|XP_008465885.1| (PREDICTED: uncharacterized protein LOC103503469 [Cucumis melo])

HSP 1 Score: 369.8 bits (948), Expect = 3.2e-99
Identity = 194/201 (96.52%), Postives = 196/201 (97.51%), Query Frame = 1


Query: 1   MTMASSSSSVFLKSPISPSPALHFHGGEFPKSQPWIRGRPRTKSVVKAQTEVRREDIVII 60
           MTMASSSSSVFLKSPISPSPALHFHGGEFPKSQPWIRGR RTKSVVKAQTEVRREDIVII
Sbjct: 1   MTMASSSSSVFLKSPISPSPALHFHGGEFPKSQPWIRGRTRTKSVVKAQTEVRREDIVII 60

Query: 61  GGGIAGLATALSLHRLGVRSLVLEQAESLRTGGTSLTLFKNGWRVLDAIGVGNVLRTQFL 120
           GG IAGLATALSLHRLGVRSLVLEQAESLRTGGTSLTLFKNGWRVLDAIGVGNVLRTQFL
Sbjct: 61  GG-IAGLATALSLHRLGVRSLVLEQAESLRTGGTSLTLFKNGWRVLDAIGVGNVLRTQFL 120

Query: 121 ELQGMVVKSEEGKQLRSFTFKDEDESQEVRAVERRTLLETLASQLPAGTIQFSSKLEAIQ 180
           E QGMVVKSEEGKQLRSFTFKDED+SQEVRAVERRTLLETLASQLPAGTIQFSSKLEAIQ
Sbjct: 121 EFQGMVVKSEEGKQLRSFTFKDEDDSQEVRAVERRTLLETLASQLPAGTIQFSSKLEAIQ 180

Query: 181 RTHQDEVKLELVDGTQLTAKV 202
           RTHQDEV LELVDGTQL AK+
Sbjct: 181 RTHQDEVNLELVDGTQLIAKI 200

[Close]

BLAST of MELO3C003328 vs. NCBI nr
Match: gi|731438902|ref|XP_002265622.3| (PREDICTED: zeaxanthin epoxidase, chloroplastic isoform X2 [Vitis vinifera])

HSP 1 Score: 251.1 bits (640), Expect = 1.7e-63
Identity = 135/210 (64.29%), Postives = 163/210 (77.62%), Query Frame = 1


Query: 2   TMASSSSSVFLKSPISPSPAL-----HFHGGEFPKSQPW----IRGRPRTKSVVKAQTEV 61
           +MA S +S+ L SP+SP  +      H     FP+SQ +     R +P + S+V+AQ  V
Sbjct: 28  SMAKSLASLLLNSPLSPPSSFSQSLHHLQAKTFPQSQSYSGVGTRTKPISASMVEAQPPV 87

Query: 62  RREDIVIIGGGIAGLATALSLHRLGVRSLVLEQAESLRTGGTSLTLFKNGWRVLDAIGVG 121
           R+EDI+I+G GIAGLATA+SLHRLGV SLVLEQAESLRTGGTSLTLFKNGW VLDA+GVG
Sbjct: 88  RKEDIIIVGAGIAGLATAVSLHRLGVGSLVLEQAESLRTGGTSLTLFKNGWGVLDAMGVG 147

Query: 122 NVLRTQFLELQGMVVKSEEGKQLRSFTFKDEDESQEVRAVERRTLLETLASQLPAGTIQF 181
           N LR+QFLE+QGMVVKSE+G++LRSF FKDEDESQEVRAVERR LLETLA+QLP  +I F
Sbjct: 148 NDLRSQFLEIQGMVVKSEDGRELRSFRFKDEDESQEVRAVERRILLETLANQLPTDSIHF 207

Query: 182 SSKLEAIQRTHQDEVKLELVDGTQLTAKVI 203
           SSKL  I+R    E  LEL DGT+L+ K++
Sbjct: 208 SSKLAKIERIETGETLLELEDGTRLSGKIV 237

[Close]

BLAST of MELO3C003328 vs. NCBI nr
Match: gi|296085721|emb|CBI29521.3| (unnamed protein product [Vitis vinifera])

HSP 1 Score: 250.8 bits (639), Expect = 2.2e-63
Identity = 135/209 (64.59%), Postives = 162/209 (77.51%), Query Frame = 1


Query: 3   MASSSSSVFLKSPISPSPAL-----HFHGGEFPKSQPW----IRGRPRTKSVVKAQTEVR 62
           MA S +S+ L SP+SP  +      H     FP+SQ +     R +P + S+V+AQ  VR
Sbjct: 1   MAKSLASLLLNSPLSPPSSFSQSLHHLQAKTFPQSQSYSGVGTRTKPISASMVEAQPPVR 60

Query: 63  REDIVIIGGGIAGLATALSLHRLGVRSLVLEQAESLRTGGTSLTLFKNGWRVLDAIGVGN 122
           +EDI+I+G GIAGLATA+SLHRLGV SLVLEQAESLRTGGTSLTLFKNGW VLDA+GVGN
Sbjct: 61  KEDIIIVGAGIAGLATAVSLHRLGVGSLVLEQAESLRTGGTSLTLFKNGWGVLDAMGVGN 120

Query: 123 VLRTQFLELQGMVVKSEEGKQLRSFTFKDEDESQEVRAVERRTLLETLASQLPAGTIQFS 182
            LR+QFLE+QGMVVKSE+G++LRSF FKDEDESQEVRAVERR LLETLA+QLP  +I FS
Sbjct: 121 DLRSQFLEIQGMVVKSEDGRELRSFRFKDEDESQEVRAVERRILLETLANQLPTDSIHFS 180

Query: 183 SKLEAIQRTHQDEVKLELVDGTQLTAKVI 203
           SKL  I+R    E  LEL DGT+L+ K++
Sbjct: 181 SKLAKIERIETGETLLELEDGTRLSGKIV 209

The following BLAST results are available for this feature:

BLAST of MELO3C003328 vs. Swiss-Prot


Match Name	E-value	Identity	Description
HPXO_ACIAD	6.9e-09	27.92	FAD-dependent urate hydroxylase OS=Acinetobacter baylyi (strain ATCC 33305 / BD4...	[more]
3HBH1_PSEAC	2.9e-07	47.54	3-hydroxybenzoate 6-hydroxylase 1 OS=Pseudomonas alcaligenes GN=xlnD PE=1 SV=1	[more]
BIK2_GIBF5	5.0e-07	25.66	FAD-dependent monooxygenase bik2 OS=Gibberella fujikuroi (strain CBS 195.34 / IM...	[more]
3HBH_KLEOX	5.0e-07	45.76	3-hydroxybenzoate 6-hydroxylase OS=Klebsiella oxytoca GN=mhbM PE=1 SV=1	[more]
ATMM_ASPFL	1.9e-06	26.14	FAD-dependent monooxygenase atmM OS=Aspergillus flavus GN=atmM PE=2 SV=1	[more]

BLAST of MELO3C003328 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LEK6_CUCSA	2.0e-100	95.54	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G912970 PE=4 SV=1	[more]
D7TG98_VITVI	1.5e-63	64.59	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_00s0533g00020 PE=4 SV=...	[more]
I3S124_LOTJA	2.4e-61	65.50	Uncharacterized protein OS=Lotus japonicus PE=2 SV=1	[more]
M5W9D2_PRUPE	3.2e-61	65.55	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa005571mg PE=4 SV=1	[more]
K4C6R8_SOLLC	6.6e-59	62.38	Uncharacterized protein OS=Solanum lycopersicum PE=4 SV=1	[more]

BLAST of MELO3C003328 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G35660.1	7.3e-57	67.26	FAD/NAD(P)-binding oxidoreductase family protein	[more]
AT2G29720.1	7.5e-54	61.24	FAD/NAD(P)-binding oxidoreductase family protein	[more]
AT5G05320.1	7.1e-28	44.59	FAD/NAD(P)-binding oxidoreductase family protein	[more]
AT4G38540.1	5.6e-25	42.86	FAD/NAD(P)-binding oxidoreductase family protein	[more]
AT4G15760.1	4.0e-23	41.40	monooxygenase 1	[more]

BLAST of MELO3C003328 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659131847\|ref\|XP_008465886.1\|	1.1e-104	99.01	PREDICTED: kynurenine 3-monooxygenase-like, partial [Cucumis melo]	[more]
gi\|778688179\|ref\|XP_004148358.2\|	2.9e-100	95.54	PREDICTED: zeaxanthin epoxidase, chloroplastic isoform X1 [Cucumis sativus]	[more]
gi\|659131845\|ref\|XP_008465885.1\|	3.2e-99	96.52	PREDICTED: uncharacterized protein LOC103503469 [Cucumis melo]	[more]
gi\|731438902\|ref\|XP_002265622.3\|	1.7e-63	64.29	PREDICTED: zeaxanthin epoxidase, chloroplastic isoform X2 [Vitis vinifera]	[more]
gi\|296085721\|emb\|CBI29521.3\|	2.2e-63	64.59	unnamed protein product [Vitis vinifera]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR002938	FAD-bd
IPR023753	FAD/NAD-binding_dom

Vocabulary: **Molecular Function**
Term	Definition
GO:0071949	FAD binding
GO:0016491	oxidoreductase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0044550	secondary metabolite biosynthetic process
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0071949	FAD binding
molecular_function	GO:0016491	oxidoreductase activity
molecular_function	GO:0052663	antheraxanthin epoxidase activity
molecular_function	GO:0052662	zeaxanthin epoxidase activity
molecular_function	GO:0009540	zeaxanthin epoxidase [overall] activity
molecular_function	GO:0004497	monooxygenase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MELO3C003328T1	MELO3C003328T1	mRNA

InterPro

Analysis Name: InterPro Annotations of melon
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002938	FAD-binding domain	PFAM	PF01494	FAD_binding_3	coord: 56..188 score: 1.
IPR023753	FAD/NAD(P)-binding domain	GENE3D	G3DSA:3.50.50.60		coord: 56..202 score: 3.7
IPR023753	FAD/NAD(P)-binding domain	unknown	SSF51905	FAD/NAD(P)-binding domain	coord: 56..201 score: 4.77
None	No IPR available	PANTHER	PTHR13789	MONOOXYGENASE	coord: 30..202 score: 4.4
None	No IPR available	PANTHER	PTHR13789:SF224	CTF2A LIKE OXIDOREDUCTASE-RELATED	coord: 30..202 score: 4.4

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
MELO3C003328	Csa3G912970	Cucumber (Chinese Long) v2	cumeB222
MELO3C003328	Cla002818	Watermelon (97103) v1	mewmB342
MELO3C003328	ClCG09G012810	Watermelon (Charleston Gray)	mewcgB310
MELO3C003328	Cla97C09G175550	Watermelon (97103) v2	mewmbB363
MELO3C003328	Bhi05G000430	Wax gourd	mewgoB407
MELO3C003328	CSPI03G47460	Wild cucumber (PI 183967)	cpimeB215
MELO3C003328	Cucsa.313810	Cucumber (Gy14) v1	cgymeB517
MELO3C003328	CmoCh02G016110	Cucurbita moschata (Rifu)	cmomeB556
MELO3C003328	Lsi05G000190	Bottle gourd (USVL1VR-Ls)	lsimeB351
MELO3C003328	Cp4.1LG05g02220	Cucurbita pepo (Zucchini)	cpemeB689
MELO3C003328	CsGy3G044830	Cucumber (Gy14) v2	cgybmeB185
MELO3C003328	CsaV3_3G049870	Cucumber (Chinese Long) v3	cucmeB219

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
MELO3C003328	Silver-seed gourd	carmeB0221
MELO3C003328	Silver-seed gourd	carmeB0568
MELO3C003328	Silver-seed gourd	carmeB0702
MELO3C003328	Silver-seed gourd	carmeB0771
MELO3C003328	Cucumber (Chinese Long) v3	cucmeB222
MELO3C003328	Watermelon (97103) v2	mewmbB318
MELO3C003328	Wax gourd	mewgoB378
MELO3C003328	Melon (DHL92) v3.5.1	memeB139
MELO3C003328	Cucumber (Gy14) v1	cgymeB531
MELO3C003328	Cucurbita maxima (Rimu)	cmameB289
MELO3C003328	Cucurbita maxima (Rimu)	cmameB469
MELO3C003328	Cucurbita maxima (Rimu)	cmameB473
MELO3C003328	Cucurbita maxima (Rimu)	cmameB567
MELO3C003328	Cucurbita maxima (Rimu)	cmameB754
MELO3C003328	Cucurbita moschata (Rifu)	cmomeB282
MELO3C003328	Cucurbita moschata (Rifu)	cmomeB459
MELO3C003328	Cucurbita moschata (Rifu)	cmomeB463
MELO3C003328	Cucurbita moschata (Rifu)	cmomeB746
MELO3C003328	Wild cucumber (PI 183967)	cpimeB218
MELO3C003328	Cucumber (Chinese Long) v2	cumeB227
MELO3C003328	Watermelon (Charleston Gray)	mewcgB317
MELO3C003328	Cucurbita pepo (Zucchini)	cpemeB233
MELO3C003328	Bottle gourd (USVL1VR-Ls)	lsimeB439
MELO3C003328	Cucumber (Gy14) v2	cgybmeB188