MELO3C002176.2 (gene) Melon (DHL92) v3.6.1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	MELO3C002176.2
Type	gene
Organism	Cucumis melo (Melon (DHL92) v3.6.1)
Description	miraculin-like
Location	chr12 : 25819020 .. 25819801 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTAAAACCAAATCGGCAAGAAAGAAAAAAAATTAGCATGAAGAAATTTGCATTACTCAGTTTTCTTTTCATTGCCATTGTCATTGCCTCATCTGAGCTACGCTTCTGCAGAGCTGATGCCTCACCGGATGCCGTCCTCGACACCGATGGAAAGAAGCTCAGAGTCAGCAACAAGTACTACATCCTCCCAGCATTTGAAGGCAGCGGTGGTGGCGGTTTAGCCATCGGCAATATCCGAAAAGAATACGATAGATGTGGTATCAACGTCGTCCAGGAGCGTTATGAACAATCCGATGGTGATCCCACGACATTTTTACCAATAAACCCTAAAAAGGGTGTGGTTCGAGTCTCCACCGATTTGAACATCGAATTTGATGCGACTACGAGGTGTAGGAAATCGACGGTGTGGAAATTAGGAACATTTGATCGATATTTGAGGCAATATTTCGTGACGATTGGCGGAACGAAAGGGAATCCGGGGCGAGAGACGACAGAAAATTGGTTCAAAATAGAGAAATATGGGAAGGGTAATTACAAGTTGGTGTATTGTCCAAGAGTGTGTAAGTATTGCAAAGTAATGTGCAAAGATATTGGTATATTTGAGAACAATGGGATAAGGGGTCTTGTTTTGAGCGATACGCCATTTCCCGTTATCTTCAAGAAAGTTTATGAATGAATTTGCAAGGATGTGATCAAGAGGGTTGGGTTTTGGGTGATCTGTCTTGAAATTTCATCCAATTCTCAATTTCTATTGTAATAATAAATCACGACTTAATTTCGTG

mRNA sequence

CTTAAAACCAAATCGGCAAGAAAGAAAAAAAATTAGCATGAAGAAATTTGCATTACTCAGTTTTCTTTTCATTGCCATTGTCATTGCCTCATCTGAGCTACGCTTCTGCAGAGCTGATGCCTCACCGGATGCCGTCCTCGACACCGATGGAAAGAAGCTCAGAGTCAGCAACAAGTACTACATCCTCCCAGCATTTGAAGGCAGCGGTGGTGGCGGTTTAGCCATCGGCAATATCCGAAAAGAATACGATAGATGTGGTATCAACGTCGTCCAGGAGCGTTATGAACAATCCGATGGTGATCCCACGACATTTTTACCAATAAACCCTAAAAAGGGTGTGGTTCGAGTCTCCACCGATTTGAACATCGAATTTGATGCGACTACGAGGTGTAGGAAATCGACGGTGTGGAAATTAGGAACATTTGATCGATATTTGAGGCAATATTTCGTGACGATTGGCGGAACGAAAGGGAATCCGGGGCGAGAGACGACAGAAAATTGGTTCAAAATAGAGAAATATGGGAAGGGTAATTACAAGTTGGTGTATTGTCCAAGAGTGTGTAAGTATTGCAAAGTAATGTGCAAAGATATTGGTATATTTGAGAACAATGGGATAAGGGGTCTTGTTTTGAGCGATACGCCATTTCCCGTTATCTTCAAGAAAGTTTATGAATGAATTTGCAAGGATGTGATCAAGAGGGTTGGGTTTTGGGTGATCTGTCTTGAAATTTCATCCAATTCTCAATTTCTATTGTAATAATAAATCACGACTTAATTTCGTG

Coding sequence (CDS)

ATGAAGAAATTTGCATTACTCAGTTTTCTTTTCATTGCCATTGTCATTGCCTCATCTGAGCTACGCTTCTGCAGAGCTGATGCCTCACCGGATGCCGTCCTCGACACCGATGGAAAGAAGCTCAGAGTCAGCAACAAGTACTACATCCTCCCAGCATTTGAAGGCAGCGGTGGTGGCGGTTTAGCCATCGGCAATATCCGAAAAGAATACGATAGATGTGGTATCAACGTCGTCCAGGAGCGTTATGAACAATCCGATGGTGATCCCACGACATTTTTACCAATAAACCCTAAAAAGGGTGTGGTTCGAGTCTCCACCGATTTGAACATCGAATTTGATGCGACTACGAGGTGTAGGAAATCGACGGTGTGGAAATTAGGAACATTTGATCGATATTTGAGGCAATATTTCGTGACGATTGGCGGAACGAAAGGGAATCCGGGGCGAGAGACGACAGAAAATTGGTTCAAAATAGAGAAATATGGGAAGGGTAATTACAAGTTGGTGTATTGTCCAAGAGTGTGTAAGTATTGCAAAGTAATGTGCAAAGATATTGGTATATTTGAGAACAATGGGATAAGGGGTCTTGTTTTGAGCGATACGCCATTTCCCGTTATCTTCAAGAAAGTTTATGAATGA

Protein sequence

MKKFALLSFLFIAIVIASSELRFCRADASPDAVLDTDGKKLRVSNKYYILPAFEGSGGGGLAIGNIRKEYDRCGINVVQERYEQSDGDPTTFLPINPKKGVVRVSTDLNIEFDATTRCRKSTVWKLGTFDRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGKGNYKLVYCPRVCKYCKVMCKDIGIFENNGIRGLVLSDTPFPVIFKKVYE

Homology

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BLAST of MELO3C002176.2 vs. NCBI nr
Match: XP_008437071.1 (PREDICTED: miraculin-like [Cucumis melo])

HSP 1 Score: 443.4 bits (1139), Expect = 4.5e-121
Identity = 212/212 (100.00%), Postives = 212/212 (100.00%), Query Frame = 0


Query: 1   MKKFALLSFLFIAIVIASSELRFCRADASPDAVLDTDGKKLRVSNKYYILPAFEGSGGGG 60
           MKKFALLSFLFIAIVIASSELRFCRADASPDAVLDTDGKKLRVSNKYYILPAFEGSGGGG
Sbjct: 1   MKKFALLSFLFIAIVIASSELRFCRADASPDAVLDTDGKKLRVSNKYYILPAFEGSGGGG 60

Query: 61  LAIGNIRKEYDRCGINVVQERYEQSDGDPTTFLPINPKKGVVRVSTDLNIEFDATTRCRK 120
           LAIGNIRKEYDRCGINVVQERYEQSDGDPTTFLPINPKKGVVRVSTDLNIEFDATTRCRK
Sbjct: 61  LAIGNIRKEYDRCGINVVQERYEQSDGDPTTFLPINPKKGVVRVSTDLNIEFDATTRCRK 120

Query: 121 STVWKLGTFDRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGKGNYKLVYCPRVCKYCKV 180
           STVWKLGTFDRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGKGNYKLVYCPRVCKYCKV
Sbjct: 121 STVWKLGTFDRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGKGNYKLVYCPRVCKYCKV 180

Query: 181 MCKDIGIFENNGIRGLVLSDTPFPVIFKKVYE 213
           MCKDIGIFENNGIRGLVLSDTPFPVIFKKVYE
Sbjct: 181 MCKDIGIFENNGIRGLVLSDTPFPVIFKKVYE 212

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BLAST of MELO3C002176.2 vs. NCBI nr
Match: XP_008437082.1 (PREDICTED: miraculin [Cucumis melo])

HSP 1 Score: 301.6 bits (771), Expect = 2.1e-78
Identity = 147/210 (70.00%), Postives = 170/210 (80.95%), Query Frame = 0


Query: 1   MKKFALLSFLFIAIVIASSELRFCRADASPDAVLDTDGKKLRVSNKYYILPAFEGSGGGG 60
           MK FALL FLF  IVIASSE+RFCRADASPDAVLDTDGKKLR  ++YYIL  +     GG
Sbjct: 1   MKNFALLCFLF--IVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVY-SRNSGG 60

Query: 61  LAIGNIRKEYDRCGINVVQERYEQSDGDPTTFLPINPKKGVVRVSTDLNIEFDATTRCRK 120
           L+IG I   Y++C IN++ E Y+  DG P TF P+NPKKGVVRVSTDLNIEF+A+TRC  
Sbjct: 61  LSIGGI-YGYEKCPINILPESYDYLDGLPATFSPVNPKKGVVRVSTDLNIEFEASTRCGI 120

Query: 121 STVWKLGTFDRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGKGNYKLVYCPRVCKYCKV 180
           STVWK+G FD+YL+QYFVT+GGTKGNPGRET  NWFK+EK+GK NYK VYCP VCKYCKV
Sbjct: 121 STVWKVGKFDQYLKQYFVTMGGTKGNPGRETIGNWFKVEKHGK-NYKFVYCPTVCKYCKV 180

Query: 181 MCKDIGIFENNGIRGLVLSDTPFPVIFKKV 211
           MCKD+G+F  NG R   L+D PFPV+FKKV
Sbjct: 181 MCKDVGLFYKNGRRIFALNDAPFPVMFKKV 205

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BLAST of MELO3C002176.2 vs. NCBI nr
Match: XP_004137639.1 (PREDICTED: miraculin [Cucumis sativus] >KGN64219.1 hypothetical protein Csa_1G043220 [Cucumis sativus])

HSP 1 Score: 299.7 bits (766), Expect = 8.0e-78
Identity = 146/210 (69.52%), Postives = 169/210 (80.48%), Query Frame = 0


Query: 1   MKKFALLSFLFIAIVIASSELRFCRADASPDAVLDTDGKKLRVSNKYYILPAFEGSGGGG 60
           M+ FALL FLF  IVIASSE+RFCRADASPDAVLDTDGKKLR  ++YYIL  +     GG
Sbjct: 1   MRNFALLCFLF--IVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVY-SRNSGG 60

Query: 61  LAIGNIRKEYDRCGINVVQERYEQSDGDPTTFLPINPKKGVVRVSTDLNIEFDATTRCRK 120
           L+IG I   Y++C IN++ E Y+   G P TF PINPKKGVVRVSTDLNI+F+A TRC  
Sbjct: 61  LSIGGI-YGYEKCPINILPESYDYLHGLPATFSPINPKKGVVRVSTDLNIQFEANTRCGI 120

Query: 121 STVWKLGTFDRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGKGNYKLVYCPRVCKYCKV 180
           STVWK+G FD YL+QYFVT+GG KGNPGRET ENWFK+EKYGK NYKLVYCP VCKYCKV
Sbjct: 121 STVWKVGKFDEYLKQYFVTMGGMKGNPGRETIENWFKVEKYGK-NYKLVYCPTVCKYCKV 180

Query: 181 MCKDIGIFENNGIRGLVLSDTPFPVIFKKV 211
           +CKD+G+F  NG R + L+D PFPV+FKKV
Sbjct: 181 VCKDVGLFYKNGRRVIALNDAPFPVMFKKV 205

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BLAST of MELO3C002176.2 vs. NCBI nr
Match: XP_022137472.1 (kunitz trypsin inhibitor 2 [Momordica charantia])

HSP 1 Score: 283.9 bits (725), Expect = 4.5e-73
Identity = 138/210 (65.71%), Postives = 166/210 (79.05%), Query Frame = 0


Query: 1   MKKFALLSFLFIAIVIASSELRFCRADASPDAVLDTDGKKLRVSNKYYILPAFEGSGGGG 60
           MK FALL FLF   +IAS+E+R C++DASPDAVLD DGKKLR    YYILP   G  GGG
Sbjct: 1   MKNFALLCFLF--ALIASTEVRICKSDASPDAVLDIDGKKLRAGVNYYILPVIRGR-GGG 60

Query: 61  LAIGNIRKEYDRCGINVVQERYEQSDGDPTTFLPINPKKGVVRVSTDLNIEFDATTRCRK 120
           L +GNI   +D+C ++VVQE++E S+G P TF P+NPKKGVVRVSTDLN+EF+A+T C K
Sbjct: 61  LTLGNI--GHDKCPLHVVQEQFEISNGLPATFAPVNPKKGVVRVSTDLNVEFEASTVCAK 120

Query: 121 STVWKLGTFDRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGKGNYKLVYCPRVCKYCKV 180
           STVWKL  FD   RQ+ V IGG +GNPG+ET +NWFKIEK+GK +YK V+CP VCK+CKV
Sbjct: 121 STVWKLDKFDESTRQWLVAIGGIRGNPGQETLDNWFKIEKHGK-DYKFVFCPSVCKFCKV 180

Query: 181 MCKDIGIFENNGIRGLVLSDTPFPVIFKKV 211
           +CKD+GIF  NG R L LSDTPFPV+FKKV
Sbjct: 181 ICKDVGIFIKNGNRALALSDTPFPVMFKKV 204

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BLAST of MELO3C002176.2 vs. NCBI nr
Match: XP_008437058.1 (PREDICTED: miraculin-like [Cucumis melo])

HSP 1 Score: 278.9 bits (712), Expect = 1.5e-71
Identity = 135/202 (66.83%), Postives = 160/202 (79.21%), Query Frame = 0


Query: 9   FLFIAIVIASSELRFCRADASPDAVLDTDGKKLRVSNKYYILPAFEGSGGGGLAIGNIRK 68
           F FI I++AS+ELRF  ADASP+AVLD DGKKLR    YYILP F G  GGGL +GN++ 
Sbjct: 8   FYFIFILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGR-GGGLTLGNLQS 67

Query: 69  EYDRCGINVVQERYEQSDGDPTTFLPINPKKGVVRVSTDLNIEFDATTRCRKSTVWKLGT 128
           E   C +NVVQE++E  +G PTTF P+NPKKGVVRVSTDLN++FDA+T C  STVWKL  
Sbjct: 68  EI--CPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVWKLDK 127

Query: 129 FDRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGKGNYKLVYCPRVCKYCKVMCKDIGIF 188
           FD    Q+FVTIGG++GNPG ET +NWFKIEK+GK +YKLV+CP VC +CKVMC+DIGIF
Sbjct: 128 FDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGK-DYKLVFCPTVCNFCKVMCRDIGIF 187

Query: 189 ENNGIRGLVLSDTPFPVIFKKV 211
             NG R L LSDTPFPV+FKKV
Sbjct: 188 FKNGKRALALSDTPFPVMFKKV 205

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BLAST of MELO3C002176.2 vs. TAIR10
Match: AT1G17860.1 (Kunitz family trypsin and protease inhibitor protein)

HSP 1 Score: 168.7 bits (426), Expect = 3.9e-42
Identity = 85/206 (41.26%), Postives = 128/206 (62.14%), Query Frame = 0


Query: 6   LLSFLFIAIVIASSELRFCRADASPDAVLDTDGKKLRVSNKYYILPAFEGSGGGGLAIGN 65
           L  FL +A+ I+    R    +A+ + V D +GK L     YYILP   G  GGGL + N
Sbjct: 5   LYIFLLLAVFISH---RGVTTEAAVEPVKDINGKSLLTGVNYYILPVIRGR-GGGLTMSN 64

Query: 66  IRKEYDRCGINVVQERYEQSDGDPTTFLPINPKKGVVRVSTDLNIEFDATTRCRKSTVWK 125
           ++ E   C  +V+Q+++E S G P  F P + K   + VSTD+NI+F  T      ++W+
Sbjct: 65  LKTE--TCPTSVIQDQFEVSQGLPVKFSPYD-KSRTIPVSTDVNIKFSPT------SIWE 124

Query: 126 LGTFDRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGKGNYKLVYCPRVCKYCKVMCKDI 185
           L  FD   +Q+F++  G +GNPG++T +NWFKI+K+ K +YK+ +CP VC +CKV+C+D+
Sbjct: 125 LANFDETTKQWFISTCGVEGNPGQKTVDNWFKIDKFEK-DYKIRFCPTVCNFCKVICRDV 184

Query: 186 GIFENNGIRGLVLSDTPFPVIFKKVY 212
           G+F  +G R L LSD P  V+FK+ Y
Sbjct: 185 GVFVQDGKRRLALSDVPLKVMFKRAY 196

[Close]

BLAST of MELO3C002176.2 vs. TAIR10
Match: AT1G73260.1 (kunitz trypsin inhibitor 1)

HSP 1 Score: 131.3 bits (329), Expect = 6.8e-31
Identity = 74/180 (41.11%), Postives = 98/180 (54.44%), Query Frame = 0


Query: 32  AVLDTDGKKLRVSNKYYILPAFEGSGGGGLAIGNIRKEYDRCGINVVQERYEQSDGDPTT 91
           AV+D DG  +     YY+LP   G GGG    G   +    C  ++VQE  E  +G P  
Sbjct: 29  AVVDIDGNAM-FHESYYVLPVIRGRGGGLTLAG---RGGQPCPYDIVQESSEVDEGIPVK 88

Query: 92  FLPINPKKGVVRVSTDLNIEFD-ATTRCRKSTVWKLGTFDRYLRQYFVTIGGTKGNPGRE 151
           F     K   V  S +LNIE D   T C +ST W++G FD   +QYFV  G      G++
Sbjct: 89  FSNWRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHERKQYFVVAGPKPEGFGQD 148

Query: 152 TTENWFKIEKYGKGNYKLVYCPRVCKYCKVMCKDIGIF-ENNGIRGLVLSDTPFPVIFKK 210
           + +++FKIEK G+  YK V+CPR C      C D+GIF +  G+R L LSD PF V+FKK
Sbjct: 149 SLKSFFKIEKSGEDAYKFVFCPRTCDSGNPKCSDVGIFIDELGVRRLALSDKPFLVMFKK 204

[Close]

BLAST of MELO3C002176.2 vs. TAIR10
Match: AT1G73325.1 (Kunitz family trypsin and protease inhibitor protein)

HSP 1 Score: 106.7 bits (265), Expect = 1.8e-23
Identity = 77/212 (36.32%), Postives = 115/212 (54.25%), Query Frame = 0


Query: 7   LSFLFIAIVIASSELRFC-RADASP-DAVLDTDGKKLRVSNKYYILPAFEGSGGGGLAIG 66
           L+  FI + + S+       ADA+P   VLD  G  ++ + +YYI+PA  G+GGG +   
Sbjct: 4   LTLSFITLTVLSAIFTAASAADATPSQVVLDIAGHPVQSNVQYYIIPAKIGTGGGLIPSN 63

Query: 67  NIRKEYDRC-GINVVQERYEQSDGDPTTFLPINPKKGVVRVSTDLNIEFDATT-RCRKST 126
                 D C  +++VQ       G P TF P+N K   V++S  LN+EFD+T   C  S 
Sbjct: 64  RNLSTQDLCLNLDIVQSSSPFVSGLPVTFSPLNTKVKHVQLSASLNLEFDSTVWLCPDSK 123

Query: 127 VWKLGTFDRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGKGNYKLVYCPRVCKYCKVMC 186
           VW++      LR+ FV+IGG KG        +WF+I++ G   YKL+YCP       V C
Sbjct: 124 VWRI-DHSVQLRKSFVSIGGQKGK-----GNSWFQIQEDGDA-YKLMYCP---ISSIVAC 183

Query: 187 KDIGI-FENNGIRGLVLS-DTPFPVIFKKVYE 213
            ++ +  +++G+R LVLS D  F V F+K Y+
Sbjct: 184 INVSLEIDDHGVRRLVLSTDQSFVVKFQKAYD 205

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BLAST of MELO3C002176.2 vs. Swiss-Prot
Match: sp|P13087|MIRA_SYNDU (Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3)

HSP 1 Score: 189.9 bits (481), Expect = 2.9e-47
Identity = 103/208 (49.52%), Postives = 123/208 (59.13%), Query Frame = 0


Query: 7   LSFLFI-AIVIASSELRFCRADASPDAVLDTDGKKLRVSNKYYILPAFEGSGGGGLAIGN 66
           LSF F+ A++ A++      AD++P+ VLD DG+KLR    YYI+P     GGG      
Sbjct: 9   LSFFFVSALLAAAANPLLSAADSAPNPVLDIDGEKLRTGTNYYIVPVLRDHGGGLTVSAT 68

Query: 67  IRKEYDRCGINVVQERYEQSDGDPTTFLPINPKKGVVRVSTDLNIEFDATTRCR--KSTV 126
                  C   VVQ R E     P  F P NPK+ VVRVSTDLNI F A   CR   STV
Sbjct: 69  TPNGTFVCPPRVVQTRKEVDHDRPLAFFPENPKEDVVRVSTDLNINFSAFMPCRWTSSTV 128

Query: 127 WKLGTFDRYLRQYFVTIGGTKGNPGRETTENWFKIEKY-GKGNYKLVYCPRVCKYCKVMC 186
           W+L  +D    QYFVTIGG KGNPG ET  +WFKIE++ G G YKLV+CP VC  CKV C
Sbjct: 129 WRLDKYDESTGQYFVTIGGVKGNPGPETISSWFKIEEFCGSGFYKLVFCPTVCGSCKVKC 188

Query: 187 KDIGIF-ENNGIRGLVLSDTPFPVIFKK 210
            D+GI+ +  G R L LSD PF   F K
Sbjct: 189 GDVGIYIDQKGRRRLALSDKPFAFEFNK 216

[Close]

BLAST of MELO3C002176.2 vs. Swiss-Prot
Match: sp|Q9LMU2|KTI2_ARATH (Kunitz trypsin inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=KTI2 PE=2 SV=1)

HSP 1 Score: 168.7 bits (426), Expect = 7.0e-41
Identity = 85/206 (41.26%), Postives = 128/206 (62.14%), Query Frame = 0


Query: 6   LLSFLFIAIVIASSELRFCRADASPDAVLDTDGKKLRVSNKYYILPAFEGSGGGGLAIGN 65
           L  FL +A+ I+    R    +A+ + V D +GK L     YYILP   G  GGGL + N
Sbjct: 5   LYIFLLLAVFISH---RGVTTEAAVEPVKDINGKSLLTGVNYYILPVIRGR-GGGLTMSN 64

Query: 66  IRKEYDRCGINVVQERYEQSDGDPTTFLPINPKKGVVRVSTDLNIEFDATTRCRKSTVWK 125
           ++ E   C  +V+Q+++E S G P  F P + K   + VSTD+NI+F  T      ++W+
Sbjct: 65  LKTE--TCPTSVIQDQFEVSQGLPVKFSPYD-KSRTIPVSTDVNIKFSPT------SIWE 124

Query: 126 LGTFDRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGKGNYKLVYCPRVCKYCKVMCKDI 185
           L  FD   +Q+F++  G +GNPG++T +NWFKI+K+ K +YK+ +CP VC +CKV+C+D+
Sbjct: 125 LANFDETTKQWFISTCGVEGNPGQKTVDNWFKIDKFEK-DYKIRFCPTVCNFCKVICRDV 184

Query: 186 GIFENNGIRGLVLSDTPFPVIFKKVY 212
           G+F  +G R L LSD P  V+FK+ Y
Sbjct: 185 GVFVQDGKRRLALSDVPLKVMFKRAY 196

[Close]

BLAST of MELO3C002176.2 vs. Swiss-Prot
Match: sp|P32765|ASP_THECC (21 kDa seed protein OS=Theobroma cacao OX=3641 GN=ASP PE=2 SV=1)

HSP 1 Score: 149.8 bits (377), Expect = 3.4e-35
Identity = 84/203 (41.38%), Postives = 112/203 (55.17%), Query Frame = 0


Query: 10  LFIAIVIASSELRFCRADASPDAVLDTDGKKLRVSNKYYILPAFEGSGGGGLAIGNIRKE 69
           + +     S    F  A+A+   VLDTDG +L+   +YY+L +  G+GGGGLA+G  R  
Sbjct: 8   VLLLFAFTSKSYFFGVANAANSPVLDTDGDELQTGVQYYVLSSISGAGGGGLALG--RAT 67

Query: 70  YDRCGINVVQERYEQSDGDPTTFLPINPKKGVVRVSTDLNIEF--DATTRCRKSTVWKLG 129
              C   VVQ R +  +G P  F   + K  VVRVSTD+NIEF       C  STVW+L 
Sbjct: 68  GQSCPEIVVQRRSDLDNGTPVIFSNADSKDDVVRVSTDVNIEFVPIRDRLCSTSTVWRLD 127

Query: 130 TFDRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGKGNYKLVYCPRVCKYCKVMCKDIGI 189
            +D    +++VT  G KG PG  T  +WFKIEK G   YK  +CP VC  C  +C DIG 
Sbjct: 128 NYDNSAGKWWVTTDGVKGEPGPNTLCSWFKIEKAGVLGYKFRFCPSVCDSCTTLCSDIGR 187

Query: 190 F-ENNGIRGLVLSDTPFPVIFKK 210
             +++G   L LSD  +  +FKK
Sbjct: 188 HSDDDGQIRLALSDNEWAWMFKK 208

[Close]

BLAST of MELO3C002176.2 vs. Swiss-Prot
Match: sp|Q8RXD5|KTI1_ARATH (Kunitz trypsin inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=KTI1 PE=2 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 1.2e-29
Identity = 74/180 (41.11%), Postives = 98/180 (54.44%), Query Frame = 0


Query: 32  AVLDTDGKKLRVSNKYYILPAFEGSGGGGLAIGNIRKEYDRCGINVVQERYEQSDGDPTT 91
           AV+D DG  +     YY+LP   G GGG    G   +    C  ++VQE  E  +G P  
Sbjct: 29  AVVDIDGNAM-FHESYYVLPVIRGRGGGLTLAG---RGGQPCPYDIVQESSEVDEGIPVK 88

Query: 92  FLPINPKKGVVRVSTDLNIEFD-ATTRCRKSTVWKLGTFDRYLRQYFVTIGGTKGNPGRE 151
           F     K   V  S +LNIE D   T C +ST W++G FD   +QYFV  G      G++
Sbjct: 89  FSNWRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHERKQYFVVAGPKPEGFGQD 148

Query: 152 TTENWFKIEKYGKGNYKLVYCPRVCKYCKVMCKDIGIF-ENNGIRGLVLSDTPFPVIFKK 210
           + +++FKIEK G+  YK V+CPR C      C D+GIF +  G+R L LSD PF V+FKK
Sbjct: 149 SLKSFFKIEKSGEDAYKFVFCPRTCDSGNPKCSDVGIFIDELGVRRLALSDKPFLVMFKK 204

[Close]

BLAST of MELO3C002176.2 vs. Swiss-Prot
Match: sp|P80691|LSPI_CARPA (Latex serine proteinase inhibitor OS=Carica papaya OX=3649 PE=1 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 5.0e-23
Identity = 65/187 (34.76%), Postives = 106/187 (56.68%), Query Frame = 0


Query: 29  SPDAVLDTDGKKLRVSNKYYILPAFEGSGGGGLAI---GNIRKEYDRCGINVVQERYEQS 88
           +P  ++D DGK +     Y+++ A  G+GGGGL +   GN +K    C ++VVQ+ ++  
Sbjct: 2   APKPIVDIDGKPVLYGVDYFVVSAIWGAGGGGLTVYGPGNKKK----CPLSVVQDPFD-- 61

Query: 89  DGDPTTFLPI-NPKKGVVRVSTDLNIEFDATTRCRKSTVWKLGTFDRYLRQYFVTIGGTK 148
           +G+P  F  I N K  +V  S DLN++F+ T  C ++T WK+  F   +  + VT+GG K
Sbjct: 62  NGEPIIFSAIKNVKDNIVFESVDLNVKFNITINCNETTAWKVDRFPGVI-GWTVTLGGEK 121

Query: 149 GNPGRETTENWFKIEKYG-KGNYKLVYCPRVCKYCKVMCKDIGI-FENNGIRGLVLSDTP 208
           G  G E+T + FKI+K G   +YK  +CP   +   + C ++ I F+   IR L+L++  
Sbjct: 122 GYHGFESTHSMFKIKKAGLPFSYKFHFCPSYPRTRLIPCNNVDIFFDKYRIRRLILTNDA 181

Query: 209 FPVIFKK 210
              +F K
Sbjct: 182 KEFVFIK 181

[Close]

BLAST of MELO3C002176.2 vs. TrEMBL
Match: tr|A0A1S3AT47|A0A1S3AT47_CUCME (miraculin-like OS=Cucumis melo OX=3656 GN=LOC103482604 PE=4 SV=1)

HSP 1 Score: 443.4 bits (1139), Expect = 3.0e-121
Identity = 212/212 (100.00%), Postives = 212/212 (100.00%), Query Frame = 0


Query: 1   MKKFALLSFLFIAIVIASSELRFCRADASPDAVLDTDGKKLRVSNKYYILPAFEGSGGGG 60
           MKKFALLSFLFIAIVIASSELRFCRADASPDAVLDTDGKKLRVSNKYYILPAFEGSGGGG
Sbjct: 1   MKKFALLSFLFIAIVIASSELRFCRADASPDAVLDTDGKKLRVSNKYYILPAFEGSGGGG 60

Query: 61  LAIGNIRKEYDRCGINVVQERYEQSDGDPTTFLPINPKKGVVRVSTDLNIEFDATTRCRK 120
           LAIGNIRKEYDRCGINVVQERYEQSDGDPTTFLPINPKKGVVRVSTDLNIEFDATTRCRK
Sbjct: 61  LAIGNIRKEYDRCGINVVQERYEQSDGDPTTFLPINPKKGVVRVSTDLNIEFDATTRCRK 120

Query: 121 STVWKLGTFDRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGKGNYKLVYCPRVCKYCKV 180
           STVWKLGTFDRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGKGNYKLVYCPRVCKYCKV
Sbjct: 121 STVWKLGTFDRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGKGNYKLVYCPRVCKYCKV 180

Query: 181 MCKDIGIFENNGIRGLVLSDTPFPVIFKKVYE 213
           MCKDIGIFENNGIRGLVLSDTPFPVIFKKVYE
Sbjct: 181 MCKDIGIFENNGIRGLVLSDTPFPVIFKKVYE 212

[Close]

BLAST of MELO3C002176.2 vs. TrEMBL
Match: tr|A0A1S3AST3|A0A1S3AST3_CUCME (miraculin OS=Cucumis melo OX=3656 GN=LOC103482610 PE=4 SV=1)

HSP 1 Score: 301.6 bits (771), Expect = 1.4e-78
Identity = 147/210 (70.00%), Postives = 170/210 (80.95%), Query Frame = 0


Query: 1   MKKFALLSFLFIAIVIASSELRFCRADASPDAVLDTDGKKLRVSNKYYILPAFEGSGGGG 60
           MK FALL FLF  IVIASSE+RFCRADASPDAVLDTDGKKLR  ++YYIL  +     GG
Sbjct: 1   MKNFALLCFLF--IVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVY-SRNSGG 60

Query: 61  LAIGNIRKEYDRCGINVVQERYEQSDGDPTTFLPINPKKGVVRVSTDLNIEFDATTRCRK 120
           L+IG I   Y++C IN++ E Y+  DG P TF P+NPKKGVVRVSTDLNIEF+A+TRC  
Sbjct: 61  LSIGGI-YGYEKCPINILPESYDYLDGLPATFSPVNPKKGVVRVSTDLNIEFEASTRCGI 120

Query: 121 STVWKLGTFDRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGKGNYKLVYCPRVCKYCKV 180
           STVWK+G FD+YL+QYFVT+GGTKGNPGRET  NWFK+EK+GK NYK VYCP VCKYCKV
Sbjct: 121 STVWKVGKFDQYLKQYFVTMGGTKGNPGRETIGNWFKVEKHGK-NYKFVYCPTVCKYCKV 180

Query: 181 MCKDIGIFENNGIRGLVLSDTPFPVIFKKV 211
           MCKD+G+F  NG R   L+D PFPV+FKKV
Sbjct: 181 MCKDVGLFYKNGRRIFALNDAPFPVMFKKV 205

[Close]

BLAST of MELO3C002176.2 vs. TrEMBL
Match: tr|A0A0A0LT81|A0A0A0LT81_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G043220 PE=4 SV=1)

HSP 1 Score: 299.7 bits (766), Expect = 5.3e-78
Identity = 146/210 (69.52%), Postives = 169/210 (80.48%), Query Frame = 0


Query: 1   MKKFALLSFLFIAIVIASSELRFCRADASPDAVLDTDGKKLRVSNKYYILPAFEGSGGGG 60
           M+ FALL FLF  IVIASSE+RFCRADASPDAVLDTDGKKLR  ++YYIL  +     GG
Sbjct: 1   MRNFALLCFLF--IVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVY-SRNSGG 60

Query: 61  LAIGNIRKEYDRCGINVVQERYEQSDGDPTTFLPINPKKGVVRVSTDLNIEFDATTRCRK 120
           L+IG I   Y++C IN++ E Y+   G P TF PINPKKGVVRVSTDLNI+F+A TRC  
Sbjct: 61  LSIGGI-YGYEKCPINILPESYDYLHGLPATFSPINPKKGVVRVSTDLNIQFEANTRCGI 120

Query: 121 STVWKLGTFDRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGKGNYKLVYCPRVCKYCKV 180
           STVWK+G FD YL+QYFVT+GG KGNPGRET ENWFK+EKYGK NYKLVYCP VCKYCKV
Sbjct: 121 STVWKVGKFDEYLKQYFVTMGGMKGNPGRETIENWFKVEKYGK-NYKLVYCPTVCKYCKV 180

Query: 181 MCKDIGIFENNGIRGLVLSDTPFPVIFKKV 211
           +CKD+G+F  NG R + L+D PFPV+FKKV
Sbjct: 181 VCKDVGLFYKNGRRVIALNDAPFPVMFKKV 205

[Close]

BLAST of MELO3C002176.2 vs. TrEMBL
Match: tr|A0A1S3ASR3|A0A1S3ASR3_CUCME (miraculin-like OS=Cucumis melo OX=3656 GN=LOC103482596 PE=4 SV=1)

HSP 1 Score: 278.9 bits (712), Expect = 9.7e-72
Identity = 135/202 (66.83%), Postives = 160/202 (79.21%), Query Frame = 0


Query: 9   FLFIAIVIASSELRFCRADASPDAVLDTDGKKLRVSNKYYILPAFEGSGGGGLAIGNIRK 68
           F FI I++AS+ELRF  ADASP+AVLD DGKKLR    YYILP F G  GGGL +GN++ 
Sbjct: 8   FYFIFILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGR-GGGLTLGNLQS 67

Query: 69  EYDRCGINVVQERYEQSDGDPTTFLPINPKKGVVRVSTDLNIEFDATTRCRKSTVWKLGT 128
           E   C +NVVQE++E  +G PTTF P+NPKKGVVRVSTDLN++FDA+T C  STVWKL  
Sbjct: 68  EI--CPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVWKLDK 127

Query: 129 FDRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGKGNYKLVYCPRVCKYCKVMCKDIGIF 188
           FD    Q+FVTIGG++GNPG ET +NWFKIEK+GK +YKLV+CP VC +CKVMC+DIGIF
Sbjct: 128 FDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGK-DYKLVFCPTVCNFCKVMCRDIGIF 187

Query: 189 ENNGIRGLVLSDTPFPVIFKKV 211
             NG R L LSDTPFPV+FKKV
Sbjct: 188 FKNGKRALALSDTPFPVMFKKV 205

[Close]

BLAST of MELO3C002176.2 vs. TrEMBL
Match: tr|A0A0A0LTW2|A0A0A0LTW2_CUCSA (Tumor-related protein OS=Cucumis sativus OX=3659 GN=Csa_1G043200 PE=4 SV=1)

HSP 1 Score: 270.4 bits (690), Expect = 3.4e-69
Identity = 135/211 (63.98%), Postives = 164/211 (77.73%), Query Frame = 0


Query: 1   MKKFALLSFLFIAIVIASSEL-RFCRADASPDAVLDTDGKKLRVSNKYYILPAFEGSGGG 60
           MK F +L F F+ I++AS++L RF  ADASP+AVLD DGKKLR    YYILP F G  GG
Sbjct: 1   MKNFGIL-FYFLFILLASTQLIRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGR-GG 60

Query: 61  GLAIGNIRKEYDRCGINVVQERYEQSDGDPTTFLPINPKKGVVRVSTDLNIEFDATTRCR 120
           GL +GN++ E  +C +NVVQE+ E  +G PTTF P+NPKKGVVRVSTDLN++F+A+T C 
Sbjct: 61  GLTLGNLQSE--KCPLNVVQEQLEVMNGFPTTFHPVNPKKGVVRVSTDLNVQFEASTICV 120

Query: 121 KSTVWKLGTFDRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGKGNYKLVYCPRVCKYCK 180
            STVWKL  FD    Q+ VTIGG++GNPG ET +NWFKIEK+GK +YKLV+CP VC +CK
Sbjct: 121 TSTVWKLDKFDESTGQWLVTIGGSRGNPGVETVDNWFKIEKHGK-DYKLVFCPTVCNFCK 180

Query: 181 VMCKDIGIFENNGIRGLVLSDTPFPVIFKKV 211
           VMC+DIGIF  NG R L LSDTPFPV+FKKV
Sbjct: 181 VMCRDIGIFFKNGERALALSDTPFPVMFKKV 206

The following BLAST results are available for this feature:

BLAST of MELO3C002176.2 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008437071.1	4.5e-121	100.00	PREDICTED: miraculin-like [Cucumis melo]	[more]
XP_008437082.1	2.1e-78	70.00	PREDICTED: miraculin [Cucumis melo]	[more]
XP_004137639.1	8.0e-78	69.52	PREDICTED: miraculin [Cucumis sativus] >KGN64219.1 hypothetical protein Csa_1G04...	[more]
XP_022137472.1	4.5e-73	65.71	kunitz trypsin inhibitor 2 [Momordica charantia]	[more]
XP_008437058.1	1.5e-71	66.83	PREDICTED: miraculin-like [Cucumis melo]	[more]

BLAST of MELO3C002176.2 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G17860.1	3.9e-42	41.26	Kunitz family trypsin and protease inhibitor protein	[more]
AT1G73260.1	6.8e-31	41.11	kunitz trypsin inhibitor 1	[more]
AT1G73325.1	1.8e-23	36.32	Kunitz family trypsin and protease inhibitor protein	[more]

BLAST of MELO3C002176.2 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|P13087\|MIRA_SYNDU	2.9e-47	49.52	Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3	[more]
sp\|Q9LMU2\|KTI2_ARATH	7.0e-41	41.26	Kunitz trypsin inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=KTI2 PE=2 SV=1	[more]
sp\|P32765\|ASP_THECC	3.4e-35	41.38	21 kDa seed protein OS=Theobroma cacao OX=3641 GN=ASP PE=2 SV=1	[more]
sp\|Q8RXD5\|KTI1_ARATH	1.2e-29	41.11	Kunitz trypsin inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=KTI1 PE=2 SV=1	[more]
sp\|P80691\|LSPI_CARPA	5.0e-23	34.76	Latex serine proteinase inhibitor OS=Carica papaya OX=3649 PE=1 SV=1	[more]

BLAST of MELO3C002176.2 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S3AT47\|A0A1S3AT47_CUCME	3.0e-121	100.00	miraculin-like OS=Cucumis melo OX=3656 GN=LOC103482604 PE=4 SV=1	[more]
tr\|A0A1S3AST3\|A0A1S3AST3_CUCME	1.4e-78	70.00	miraculin OS=Cucumis melo OX=3656 GN=LOC103482610 PE=4 SV=1	[more]
tr\|A0A0A0LT81\|A0A0A0LT81_CUCSA	5.3e-78	69.52	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G043220 PE=4 SV=1	[more]
tr\|A0A1S3ASR3\|A0A1S3ASR3_CUCME	9.7e-72	66.83	miraculin-like OS=Cucumis melo OX=3656 GN=LOC103482596 PE=4 SV=1	[more]
tr\|A0A0A0LTW2\|A0A0A0LTW2_CUCSA	3.4e-69	63.98	Tumor-related protein OS=Cucumis sativus OX=3659 GN=Csa_1G043200 PE=4 SV=1	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004866	endopeptidase inhibitor activity

Vocabulary: **INTERPRO**
Term	Definition
IPR011065	Kunitz_inhibitor_STI-like_sf
IPR002160	Prot_inh_Kunz-lg

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004866	endopeptidase inhibitor activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MELO3C002176.2.1	MELO3C002176.2.1	mRNA

InterPro

Analysis Name: InterPro Annotations of melon v3.6.1
Date Performed: 2018-09-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002160	Proteinase inhibitor I3, Kunitz legume	PRINTS	PR00291	KUNITZINHBTR	coord: 155..174 score: 28.73 coord: 73..93 score: 28.38 coord: 179..208 score: 23.66 coord: 32..61 score: 41.96
IPR002160	Proteinase inhibitor I3, Kunitz legume	SMART	SM00452	kul_2	coord: 32..211 e-value: 2.1E-67 score: 239.9
IPR002160	Proteinase inhibitor I3, Kunitz legume	PFAM	PF00197	Kunitz_legume	coord: 33..209 e-value: 2.3E-53 score: 180.7
IPR002160	Proteinase inhibitor I3, Kunitz legume	PANTHER	PTHR33107	FAMILY NOT NAMED	coord: 1..210
IPR002160	Proteinase inhibitor I3, Kunitz legume	PROSITE	PS00283	SOYBEAN_KUNITZ	coord: 33..49
IPR002160	Proteinase inhibitor I3, Kunitz legume	CDD	cd00178	STI	coord: 32..209 e-value: 1.72988E-52 score: 167.904
None	No IPR available	GENE3D	G3DSA:2.80.10.50		coord: 30..212 e-value: 3.9E-68 score: 230.8
IPR011065	Kunitz inhibitor STI-like superfamily	SUPERFAMILY	SSF50386	STI-like	coord: 29..210

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
MELO3C002176.2	Cucumber (Gy14) v2	cgybmedB020
MELO3C002176.2	Cucumber (Gy14) v1	cgymedB182
MELO3C002176.2	Cucurbita maxima (Rimu)	cmamedB214
MELO3C002176.2	Cucurbita maxima (Rimu)	cmamedB424
MELO3C002176.2	Cucurbita moschata (Rifu)	cmomedB202
MELO3C002176.2	Cucurbita moschata (Rifu)	cmomedB408
MELO3C002176.2	Cucurbita pepo (Zucchini)	cpemedB031
MELO3C002176.2	Cucurbita pepo (Zucchini)	cpemedB560
MELO3C002176.2	Cucumber (Chinese Long) v2	cumedB022
MELO3C002176.2	Wild cucumber (PI 183967)	cpimedB023
MELO3C002176.2	Bottle gourd (USVL1VR-Ls)	lsimedB189
MELO3C002176.2	Watermelon (Charleston Gray)	medwcgB125
MELO3C002176.2	Watermelon (97103) v1	medwmB163
MELO3C002176.2	Silver-seed gourd	carmedB0397
MELO3C002176.2	Silver-seed gourd	carmedB0504
MELO3C002176.2	Cucumber (Chinese Long) v3	cucmedB022
MELO3C002176.2	Watermelon (97103) v2	medwmbB125
MELO3C002176.2	Wax gourd	medwgoB175