MELO3C002175.2 (gene) Melon (DHL92) v3.6.1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	MELO3C002175.2
Type	gene
Organism	Cucumis melo (Melon (DHL92) v3.6.1)
Description	Kunitz trypsin inhibitor
Location	chr12 : 25823384 .. 25824252 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TAAGCATCCAAACAAAACAAAGTCCACATTCAAAAGAAAAATGACGAAGAATTTCGGAATATTCTATTTTATTTTCATTCTCCTTGCCTCAACCGAGCTCCGCTTCTCCACAGCCGACGCTTCACCGGAGGCCGTCCTCGATATCGACGGCAAGAAGCTCCGAGCCGGCGTCAACTACTACATCCTCCCTGTTTTCCGCGGCAGAGGCGGCGGCCTAACTCTAGGCAACCTCCAATCGGAGATATGTCCAGTCAACGTCGTTCAAGAACAATTCGAATTAATGAACGGATTTCCAACAACATTCCATCCTGTAAACCCTAAAAAAGGAGTGGTTCGAGTTTCAACCGATTTGAATGTGCAATTCGATGCGAGTACGATCTGCGTGACATCGACGGTGTGGAAATTGGACAAATTCGACGAATCGACGGGACAATGGTTCGTGACGATCGGCGGAAGCAGGGGAAATCCGGGAGTGGAGACGGTGGATAATTGGTTCAAAATTGAGAAGCACGGTAAGGATTACAAATTGGTGTTCTGTCCGACTGTTTGTAATTTCTGTAAAGTTATGTGTAGAGATATTGGAATCTTCTTCAAGAATGGAAAAAGGGCTTTGGCTTTGAGCGATACGCCATTCCCTGTTATGTTCAAGAAAGTTTAATTGAAGAAAATTCAAAAATTAAAGTGAATGAGTTTATGAAGTAATTTGTGTTTAATTCATGTATAAGAGTTTTATCATAATTAAATAAAAGATATGGTTATTGTTTGTTTATTATTATTAGAAGAATAAAATACTTTATTTGATTATTTTCGTGTAAGCTGTTTTCACCTCAGTTTCTCAAAATCAAAGTACGTCTTATTTTCGTACTTAT

mRNA sequence

TAAGCATCCAAACAAAACAAAGTCCACATTCAAAAGAAAAATGACGAAGAATTTCGGAATATTCTATTTTATTTTCATTCTCCTTGCCTCAACCGAGCTCCGCTTCTCCACAGCCGACGCTTCACCGGAGGCCGTCCTCGATATCGACGGCAAGAAGCTCCGAGCCGGCGTCAACTACTACATCCTCCCTGTTTTCCGCGGCAGAGGCGGCGGCCTAACTCTAGGCAACCTCCAATCGGAGATATGTCCAGTCAACGTCGTTCAAGAACAATTCGAATTAATGAACGGATTTCCAACAACATTCCATCCTGTAAACCCTAAAAAAGGAGTGGTTCGAGTTTCAACCGATTTGAATGTGCAATTCGATGCGAGTACGATCTGCGTGACATCGACGGTGTGGAAATTGGACAAATTCGACGAATCGACGGGACAATGGTTCGTGACGATCGGCGGAAGCAGGGGAAATCCGGGAGTGGAGACGGTGGATAATTGGTTCAAAATTGAGAAGCACGGTAAGGATTACAAATTGGTGTTCTGTCCGACTGTTTGTAATTTCTGTAAAGTTATGTGTAGAGATATTGGAATCTTCTTCAAGAATGGAAAAAGGGCTTTGGCTTTGAGCGATACGCCATTCCCTGTTATGTTCAAGAAAGTTTAATTGAAGAAAATTCAAAAATTAAAGTGAATGAGTTTATGAAGTAATTTGTGTTTAATTCATGTATAAGAGTTTTATCATAATTAAATAAAAGATATGGTTATTGTTTGTTTATTATTATTAGAAGAATAAAATACTTTATTTGATTATTTTCGTGTAAGCTGTTTTCACCTCAGTTTCTCAAAATCAAAGTACGTCTTATTTTCGTACTTAT

Coding sequence (CDS)

ATGACGAAGAATTTCGGAATATTCTATTTTATTTTCATTCTCCTTGCCTCAACCGAGCTCCGCTTCTCCACAGCCGACGCTTCACCGGAGGCCGTCCTCGATATCGACGGCAAGAAGCTCCGAGCCGGCGTCAACTACTACATCCTCCCTGTTTTCCGCGGCAGAGGCGGCGGCCTAACTCTAGGCAACCTCCAATCGGAGATATGTCCAGTCAACGTCGTTCAAGAACAATTCGAATTAATGAACGGATTTCCAACAACATTCCATCCTGTAAACCCTAAAAAAGGAGTGGTTCGAGTTTCAACCGATTTGAATGTGCAATTCGATGCGAGTACGATCTGCGTGACATCGACGGTGTGGAAATTGGACAAATTCGACGAATCGACGGGACAATGGTTCGTGACGATCGGCGGAAGCAGGGGAAATCCGGGAGTGGAGACGGTGGATAATTGGTTCAAAATTGAGAAGCACGGTAAGGATTACAAATTGGTGTTCTGTCCGACTGTTTGTAATTTCTGTAAAGTTATGTGTAGAGATATTGGAATCTTCTTCAAGAATGGAAAAAGGGCTTTGGCTTTGAGCGATACGCCATTCCCTGTTATGTTCAAGAAAGTTTAA

Protein sequence

MTKNFGIFYFIFILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLGNLQSEICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVWKLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIGIFFKNGKRALALSDTPFPVMFKKV

Homology

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BLAST of MELO3C002175.2 vs. NCBI nr
Match: XP_008437058.1 (PREDICTED: miraculin-like [Cucumis melo])

HSP 1 Score: 430.3 bits (1105), Expect = 3.8e-117
Identity = 205/205 (100.00%), Postives = 205/205 (100.00%), Query Frame = 0


Query: 1   MTKNFGIFYFIFILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLT 60
           MTKNFGIFYFIFILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLT
Sbjct: 1   MTKNFGIFYFIFILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLT 60

Query: 61  LGNLQSEICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVW 120
           LGNLQSEICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVW
Sbjct: 61  LGNLQSEICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVW 120

Query: 121 KLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDI 180
           KLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDI
Sbjct: 121 KLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDI 180

Query: 181 GIFFKNGKRALALSDTPFPVMFKKV 206
           GIFFKNGKRALALSDTPFPVMFKKV
Sbjct: 181 GIFFKNGKRALALSDTPFPVMFKKV 205

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BLAST of MELO3C002175.2 vs. NCBI nr
Match: XP_004137534.1 (PREDICTED: miraculin-like [Cucumis sativus] >KGN64217.1 Tumor-related protein [Cucumis sativus])

HSP 1 Score: 402.5 bits (1033), Expect = 8.5e-109
Identity = 194/205 (94.63%), Postives = 200/205 (97.56%), Query Frame = 0


Query: 3   KNFGI-FYFIFILLASTEL-RFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLT 62
           KNFGI FYF+FILLAST+L RFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLT
Sbjct: 2   KNFGILFYFLFILLASTQLIRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLT 61

Query: 63  LGNLQSEICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVW 122
           LGNLQSE CP+NVVQEQ E+MNGFPTTFHPVNPKKGVVRVSTDLNVQF+ASTICVTSTVW
Sbjct: 62  LGNLQSEKCPLNVVQEQLEVMNGFPTTFHPVNPKKGVVRVSTDLNVQFEASTICVTSTVW 121

Query: 123 KLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDI 182
           KLDKFDESTGQW VTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDI
Sbjct: 122 KLDKFDESTGQWLVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDI 181

Query: 183 GIFFKNGKRALALSDTPFPVMFKKV 206
           GIFFKNG+RALALSDTPFPVMFKKV
Sbjct: 182 GIFFKNGERALALSDTPFPVMFKKV 206

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BLAST of MELO3C002175.2 vs. NCBI nr
Match: XP_022923703.1 (kunitz trypsin inhibitor 2 [Cucurbita moschata] >XP_023001808.1 kunitz trypsin inhibitor 2 [Cucurbita maxima])

HSP 1 Score: 364.4 bits (934), Expect = 2.6e-97
Identity = 172/203 (84.73%), Postives = 185/203 (91.13%), Query Frame = 0


Query: 3   KNFGIFYFIFILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLG 62
           KNF +  F+FILLAS E+R S ADASP+AV DIDGKKLRAGVNYYILPVFRGRGGGL LG
Sbjct: 2   KNFALLSFLFILLAS-EVRVSRADASPDAVRDIDGKKLRAGVNYYILPVFRGRGGGLALG 61

Query: 63  NLQSEICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVWKL 122
           NLQS+ CP+NVVQEQFELMNG P  F PVNPKKGVVRVSTDLNVQF+ASTIC TSTVWKL
Sbjct: 62  NLQSDKCPLNVVQEQFELMNGLPAAFLPVNPKKGVVRVSTDLNVQFEASTICATSTVWKL 121

Query: 123 DKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIGI 182
           DKFDEST QWF+TIGG+RGNPGV+TVDNWFKIEKHG DYK  FCPTVC+FCKVMCRD+GI
Sbjct: 122 DKFDESTKQWFITIGGTRGNPGVKTVDNWFKIEKHGNDYKFKFCPTVCDFCKVMCRDVGI 181

Query: 183 FFKNGKRALALSDTPFPVMFKKV 206
           FFKNGKRALALSDTPFPVMFK+V
Sbjct: 182 FFKNGKRALALSDTPFPVMFKEV 203

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BLAST of MELO3C002175.2 vs. NCBI nr
Match: XP_023520252.1 (kunitz trypsin inhibitor 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 363.2 bits (931), Expect = 5.7e-97
Identity = 172/203 (84.73%), Postives = 184/203 (90.64%), Query Frame = 0


Query: 3   KNFGIFYFIFILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLG 62
           KNF +  F+FILLAS E+R S ADASP+AV DIDGKKLRAGVNYYILPVFRGRGGGL LG
Sbjct: 2   KNFALLSFLFILLAS-EVRVSRADASPDAVRDIDGKKLRAGVNYYILPVFRGRGGGLALG 61

Query: 63  NLQSEICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVWKL 122
           NLQS+ CP+NVVQEQFELMNG P  F PVNPKKGVVRVSTDLNVQF+ASTIC TSTVWKL
Sbjct: 62  NLQSDKCPLNVVQEQFELMNGLPAAFLPVNPKKGVVRVSTDLNVQFEASTICATSTVWKL 121

Query: 123 DKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIGI 182
           DKFDEST QWF+TIGG+RGNPGV+TVDNWFKIEKHG DYK  FCPTVC+FCKVMCRD+GI
Sbjct: 122 DKFDESTKQWFITIGGARGNPGVKTVDNWFKIEKHGNDYKFKFCPTVCDFCKVMCRDVGI 181

Query: 183 FFKNGKRALALSDTPFPVMFKKV 206
           FFKNGKRALALSDTPFPVMFK V
Sbjct: 182 FFKNGKRALALSDTPFPVMFKVV 203

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BLAST of MELO3C002175.2 vs. NCBI nr
Match: XP_022137472.1 (kunitz trypsin inhibitor 2 [Momordica charantia])

HSP 1 Score: 345.5 bits (885), Expect = 1.2e-91
Identity = 158/203 (77.83%), Postives = 177/203 (87.19%), Query Frame = 0


Query: 3   KNFGIFYFIFILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLG 62
           KNF +  F+F L+ASTE+R   +DASP+AVLDIDGKKLRAGVNYYILPV RGRGGGLTLG
Sbjct: 2   KNFALLCFLFALIASTEVRICKSDASPDAVLDIDGKKLRAGVNYYILPVIRGRGGGLTLG 61

Query: 63  NLQSEICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVWKL 122
           N+  + CP++VVQEQFE+ NG P TF PVNPKKGVVRVSTDLNV+F+AST+C  STVWKL
Sbjct: 62  NIGHDKCPLHVVQEQFEISNGLPATFAPVNPKKGVVRVSTDLNVEFEASTVCAKSTVWKL 121

Query: 123 DKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIGI 182
           DKFDEST QW V IGG RGNPG ET+DNWFKIEKHGKDYK VFCP+VC FCKV+C+D+GI
Sbjct: 122 DKFDESTRQWLVAIGGIRGNPGQETLDNWFKIEKHGKDYKFVFCPSVCKFCKVICKDVGI 181

Query: 183 FFKNGKRALALSDTPFPVMFKKV 206
           F KNG RALALSDTPFPVMFKKV
Sbjct: 182 FIKNGNRALALSDTPFPVMFKKV 204

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BLAST of MELO3C002175.2 vs. TAIR10
Match: AT1G17860.1 (Kunitz family trypsin and protease inhibitor protein)

HSP 1 Score: 232.3 bits (591), Expect = 2.8e-61
Identity = 110/196 (56.12%), Postives = 142/196 (72.45%), Query Frame = 0


Query: 10  FIFILLA-STELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLGNLQSEI 69
           +IF+LLA     R  T +A+ E V DI+GK L  GVNYYILPV RGRGGGLT+ NL++E 
Sbjct: 6   YIFLLLAVFISHRGVTTEAAVEPVKDINGKSLLTGVNYYILPVIRGRGGGLTMSNLKTET 65

Query: 70  CPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVWKLDKFDES 129
           CP +V+Q+QFE+  G P  F P + K   + VSTD+N++F  ++I      W+L  FDE+
Sbjct: 66  CPTSVIQDQFEVSQGLPVKFSPYD-KSRTIPVSTDVNIKFSPTSI------WELANFDET 125

Query: 130 TGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIGIFFKNGK 189
           T QWF++  G  GNPG +TVDNWFKI+K  KDYK+ FCPTVCNFCKV+CRD+G+F ++GK
Sbjct: 126 TKQWFISTCGVEGNPGQKTVDNWFKIDKFEKDYKIRFCPTVCNFCKVICRDVGVFVQDGK 185

Query: 190 RALALSDTPFPVMFKK 205
           R LALSD P  VMFK+
Sbjct: 186 RRLALSDVPLKVMFKR 194

[Close]

BLAST of MELO3C002175.2 vs. TAIR10
Match: AT1G73260.1 (kunitz trypsin inhibitor 1)

HSP 1 Score: 151.4 bits (381), Expect = 6.2e-37
Identity = 86/196 (43.88%), Postives = 115/196 (58.67%), Query Frame = 0


Query: 12  FILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLGNLQSEICPV 71
           +++LA T +  S A     AV+DIDG  +    +YY+LPV RGRGGGLTL     + CP 
Sbjct: 13  YLVLALTAVLASNAYG---AVVDIDGNAM-FHESYYVLPVIRGRGGGLTLAGRGGQPCPY 72

Query: 72  NVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFD-ASTICVTSTVWKLDKFDESTG 131
           ++VQE  E+  G P  F     K   V  S +LN++ D  +TIC+ ST W++ +FD    
Sbjct: 73  DIVQESSEVDEGIPVKFSNWRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHERK 132

Query: 132 QWFVTIGGSRGNPGVETVDNWFKIEKHGKD-YKLVFCPTVCNFCKVMCRDIGIFFKN-GK 191
           Q+FV  G      G +++ ++FKIEK G+D YK VFCP  C+     C D+GIF    G 
Sbjct: 133 QYFVVAGPKPEGFGQDSLKSFFKIEKSGEDAYKFVFCPRTCDSGNPKCSDVGIFIDELGV 192

Query: 192 RALALSDTPFPVMFKK 205
           R LALSD PF VMFKK
Sbjct: 193 RRLALSDKPFLVMFKK 204

[Close]

BLAST of MELO3C002175.2 vs. TAIR10
Match: AT1G73325.1 (Kunitz family trypsin and protease inhibitor protein)

HSP 1 Score: 108.2 bits (269), Expect = 6.0e-24
Identity = 69/190 (36.32%), Postives = 107/190 (56.32%), Query Frame = 0


Query: 23  STADASP-EAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLGNLQ---SEIC-PVNVVQEQ 82
           S ADA+P + VLDI G  +++ V YYI+P   G GGGL   N      ++C  +++VQ  
Sbjct: 22  SAADATPSQVVLDIAGHPVQSNVQYYIIPAKIGTGGGLIPSNRNLSTQDLCLNLDIVQSS 81

Query: 83  FELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDAST-ICVTSTVWKLDKFDESTGQWFVTI 142
              ++G P TF P+N K   V++S  LN++FD++  +C  S VW++D       + FV+I
Sbjct: 82  SPFVSGLPVTFSPLNTKVKHVQLSASLNLEFDSTVWLCPDSKVWRID-HSVQLRKSFVSI 141

Query: 143 GGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIGI-FFKNGKRALALS- 202
           GG +G       ++WF+I++ G  YKL++CP       V C ++ +    +G R L LS 
Sbjct: 142 GGQKGKG-----NSWFQIQEDGDAYKLMYCPI---SSIVACINVSLEIDDHGVRRLVLST 201

Query: 203 DTPFPVMFKK 205
           D  F V F+K
Sbjct: 202 DQSFVVKFQK 202

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BLAST of MELO3C002175.2 vs. Swiss-Prot
Match: sp|Q9LMU2|KTI2_ARATH (Kunitz trypsin inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=KTI2 PE=2 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 5.0e-60
Identity = 110/196 (56.12%), Postives = 142/196 (72.45%), Query Frame = 0


Query: 10  FIFILLA-STELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLGNLQSEI 69
           +IF+LLA     R  T +A+ E V DI+GK L  GVNYYILPV RGRGGGLT+ NL++E 
Sbjct: 6   YIFLLLAVFISHRGVTTEAAVEPVKDINGKSLLTGVNYYILPVIRGRGGGLTMSNLKTET 65

Query: 70  CPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVWKLDKFDES 129
           CP +V+Q+QFE+  G P  F P + K   + VSTD+N++F  ++I      W+L  FDE+
Sbjct: 66  CPTSVIQDQFEVSQGLPVKFSPYD-KSRTIPVSTDVNIKFSPTSI------WELANFDET 125

Query: 130 TGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIGIFFKNGK 189
           T QWF++  G  GNPG +TVDNWFKI+K  KDYK+ FCPTVCNFCKV+CRD+G+F ++GK
Sbjct: 126 TKQWFISTCGVEGNPGQKTVDNWFKIDKFEKDYKIRFCPTVCNFCKVICRDVGVFVQDGK 185

Query: 190 RALALSDTPFPVMFKK 205
           R LALSD P  VMFK+
Sbjct: 186 RRLALSDVPLKVMFKR 194

[Close]

BLAST of MELO3C002175.2 vs. Swiss-Prot
Match: sp|P13087|MIRA_SYNDU (Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3)

HSP 1 Score: 217.6 bits (553), Expect = 1.3e-55
Identity = 113/206 (54.85%), Postives = 139/206 (67.48%), Query Frame = 0


Query: 8   FYFIFILL-ASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLGNLQS 67
           F+F+  LL A+     S AD++P  VLDIDG+KLR G NYYI+PV R  GGGLT+     
Sbjct: 11  FFFVSALLAAAANPLLSAADSAPNPVLDIDGEKLRTGTNYYIVPVLRDHGGGLTVSATTP 70

Query: 68  E---ICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTIC--VTSTVWK 127
               +CP  VVQ + E+ +  P  F P NPK+ VVRVSTDLN+ F A   C   +STVW+
Sbjct: 71  NGTFVCPPRVVQTRKEVDHDRPLAFFPENPKEDVVRVSTDLNINFSAFMPCRWTSSTVWR 130

Query: 128 LDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKH--GKDYKLVFCPTVCNFCKVMCRD 187
           LDK+DESTGQ+FVTIGG +GNPG ET+ +WFKIE+      YKLVFCPTVC  CKV C D
Sbjct: 131 LDKYDESTGQYFVTIGGVKGNPGPETISSWFKIEEFCGSGFYKLVFCPTVCGSCKVKCGD 190

Query: 188 IGIFF-KNGKRALALSDTPFPVMFKK 205
           +GI+  + G+R LALSD PF   F K
Sbjct: 191 VGIYIDQKGRRRLALSDKPFAFEFNK 216

[Close]

BLAST of MELO3C002175.2 vs. Swiss-Prot
Match: sp|P32765|ASP_THECC (21 kDa seed protein OS=Theobroma cacao OX=3641 GN=ASP PE=2 SV=1)

HSP 1 Score: 170.2 bits (430), Expect = 2.3e-41
Identity = 89/188 (47.34%), Postives = 115/188 (61.17%), Query Frame = 0


Query: 22  FSTADASPEAVLDIDGKKLRAGVNYYILPVFRGR-GGGLTLGNLQSEICPVNVVQEQFEL 81
           F  A+A+   VLD DG +L+ GV YY+L    G  GGGL LG    + CP  VVQ + +L
Sbjct: 21  FGVANAANSPVLDTDGDELQTGVQYYVLSSISGAGGGGLALGRATGQSCPEIVVQRRSDL 80

Query: 82  MNGFPTTFHPVNPKKGVVRVSTDLNVQF--DASTICVTSTVWKLDKFDESTGQWFVTIGG 141
            NG P  F   + K  VVRVSTD+N++F      +C TSTVW+LD +D S G+W+VT  G
Sbjct: 81  DNGTPVIFSNADSKDDVVRVSTDVNIEFVPIRDRLCSTSTVWRLDNYDNSAGKWWVTTDG 140

Query: 142 SRGNPGVETVDNWFKIEKHG-KDYKLVFCPTVCNFCKVMCRDIGIFF-KNGKRALALSDT 201
            +G PG  T+ +WFKIEK G   YK  FCP+VC+ C  +C DIG     +G+  LALSD 
Sbjct: 141 VKGEPGPNTLCSWFKIEKAGVLGYKFRFCPSVCDSCTTLCSDIGRHSDDDGQIRLALSDN 200

Query: 202 PFPVMFKK 205
            +  MFKK
Sbjct: 201 EWAWMFKK 208

[Close]

BLAST of MELO3C002175.2 vs. Swiss-Prot
Match: sp|Q8RXD5|KTI1_ARATH (Kunitz trypsin inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=KTI1 PE=2 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 1.1e-35
Identity = 86/196 (43.88%), Postives = 115/196 (58.67%), Query Frame = 0


Query: 12  FILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLGNLQSEICPV 71
           +++LA T +  S A     AV+DIDG  +    +YY+LPV RGRGGGLTL     + CP 
Sbjct: 13  YLVLALTAVLASNAYG---AVVDIDGNAM-FHESYYVLPVIRGRGGGLTLAGRGGQPCPY 72

Query: 72  NVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFD-ASTICVTSTVWKLDKFDESTG 131
           ++VQE  E+  G P  F     K   V  S +LN++ D  +TIC+ ST W++ +FD    
Sbjct: 73  DIVQESSEVDEGIPVKFSNWRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHERK 132

Query: 132 QWFVTIGGSRGNPGVETVDNWFKIEKHGKD-YKLVFCPTVCNFCKVMCRDIGIFFKN-GK 191
           Q+FV  G      G +++ ++FKIEK G+D YK VFCP  C+     C D+GIF    G 
Sbjct: 133 QYFVVAGPKPEGFGQDSLKSFFKIEKSGEDAYKFVFCPRTCDSGNPKCSDVGIFIDELGV 192

Query: 192 RALALSDTPFPVMFKK 205
           R LALSD PF VMFKK
Sbjct: 193 RRLALSDKPFLVMFKK 204

[Close]

BLAST of MELO3C002175.2 vs. Swiss-Prot
Match: sp|P29421|IAAS_ORYSJ (Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica OX=39947 GN=RASI PE=1 SV=2)

HSP 1 Score: 115.2 bits (287), Expect = 8.8e-25
Identity = 74/196 (37.76%), Postives = 103/196 (52.55%), Query Frame = 0


Query: 13  ILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLGNLQSEICPVN 72
           ILL+   + FS   A+P  V D +G +L A  +YY+LP   G GGGLT+   +   CP+ 
Sbjct: 9   ILLSLLAISFS-CSAAPPPVYDTEGHELSADGSYYVLPASPGHGGGLTMAP-RVLPCPLL 68

Query: 73  VVQEQFELMNGFPTTFHP----VNPKKGVVRVSTDLNVQFDASTICVTSTVWKLDKFDES 132
           V QE  E   GFP  F P      P+   +RVSTD+ ++F+A+TICV ST W +   +  
Sbjct: 69  VAQETDERRKGFPVRFTPWGGAAAPEDRTIRVSTDVRIRFNAATICVQSTEWHVGD-EPL 128

Query: 133 TGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIGIFFKNGK 192
           TG   V  G   G P     +N F++EK+G  YKLV        C+  C+D+G+     +
Sbjct: 129 TGARRVVTGPLIG-PSPSGRENAFRVEKYGGGYKLV-------SCRDSCQDLGVSRDGAR 188

Query: 193 RALALSDTPFPVMFKK 205
             L  S  P  V+FKK
Sbjct: 189 AWLGASQPPHVVVFKK 193

[Close]

BLAST of MELO3C002175.2 vs. TrEMBL
Match: tr|A0A1S3ASR3|A0A1S3ASR3_CUCME (miraculin-like OS=Cucumis melo OX=3656 GN=LOC103482596 PE=4 SV=1)

HSP 1 Score: 430.3 bits (1105), Expect = 2.5e-117
Identity = 205/205 (100.00%), Postives = 205/205 (100.00%), Query Frame = 0


Query: 1   MTKNFGIFYFIFILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLT 60
           MTKNFGIFYFIFILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLT
Sbjct: 1   MTKNFGIFYFIFILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLT 60

Query: 61  LGNLQSEICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVW 120
           LGNLQSEICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVW
Sbjct: 61  LGNLQSEICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVW 120

Query: 121 KLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDI 180
           KLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDI
Sbjct: 121 KLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDI 180

Query: 181 GIFFKNGKRALALSDTPFPVMFKKV 206
           GIFFKNGKRALALSDTPFPVMFKKV
Sbjct: 181 GIFFKNGKRALALSDTPFPVMFKKV 205

[Close]

BLAST of MELO3C002175.2 vs. TrEMBL
Match: tr|A0A0A0LTW2|A0A0A0LTW2_CUCSA (Tumor-related protein OS=Cucumis sativus OX=3659 GN=Csa_1G043200 PE=4 SV=1)

HSP 1 Score: 402.5 bits (1033), Expect = 5.6e-109
Identity = 194/205 (94.63%), Postives = 200/205 (97.56%), Query Frame = 0


Query: 3   KNFGI-FYFIFILLASTEL-RFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLT 62
           KNFGI FYF+FILLAST+L RFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLT
Sbjct: 2   KNFGILFYFLFILLASTQLIRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLT 61

Query: 63  LGNLQSEICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVW 122
           LGNLQSE CP+NVVQEQ E+MNGFPTTFHPVNPKKGVVRVSTDLNVQF+ASTICVTSTVW
Sbjct: 62  LGNLQSEKCPLNVVQEQLEVMNGFPTTFHPVNPKKGVVRVSTDLNVQFEASTICVTSTVW 121

Query: 123 KLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDI 182
           KLDKFDESTGQW VTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDI
Sbjct: 122 KLDKFDESTGQWLVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDI 181

Query: 183 GIFFKNGKRALALSDTPFPVMFKKV 206
           GIFFKNG+RALALSDTPFPVMFKKV
Sbjct: 182 GIFFKNGERALALSDTPFPVMFKKV 206

[Close]

BLAST of MELO3C002175.2 vs. TrEMBL
Match: tr|A0A1S3AST3|A0A1S3AST3_CUCME (miraculin OS=Cucumis melo OX=3656 GN=LOC103482610 PE=4 SV=1)

HSP 1 Score: 292.4 bits (747), Expect = 8.2e-76
Identity = 128/204 (62.75%), Postives = 166/204 (81.37%), Query Frame = 0


Query: 3   KNFGIFYFIFILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLG 62
           KNF +  F+FI++AS+E+RF  ADASP+AVLD DGKKLRAG  YYIL V+    GGL++G
Sbjct: 2   KNFALLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLSIG 61

Query: 63  NLQS-EICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVWK 122
            +   E CP+N++ E ++ ++G P TF PVNPKKGVVRVSTDLN++F+AST C  STVWK
Sbjct: 62  GIYGYEKCPINILPESYDYLDGLPATFSPVNPKKGVVRVSTDLNIEFEASTRCGISTVWK 121

Query: 123 LDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIG 182
           + KFD+   Q+FVT+GG++GNPG ET+ NWFK+EKHGK+YK V+CPTVC +CKVMC+D+G
Sbjct: 122 VGKFDQYLKQYFVTMGGTKGNPGRETIGNWFKVEKHGKNYKFVYCPTVCKYCKVMCKDVG 181

Query: 183 IFFKNGKRALALSDTPFPVMFKKV 206
           +F+KNG+R  AL+D PFPVMFKKV
Sbjct: 182 LFYKNGRRIFALNDAPFPVMFKKV 205

[Close]

BLAST of MELO3C002175.2 vs. TrEMBL
Match: tr|A0A0A0LT81|A0A0A0LT81_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G043220 PE=4 SV=1)

HSP 1 Score: 291.2 bits (744), Expect = 1.8e-75
Identity = 126/204 (61.76%), Postives = 168/204 (82.35%), Query Frame = 0


Query: 3   KNFGIFYFIFILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLG 62
           +NF +  F+FI++AS+E+RF  ADASP+AVLD DGKKLRAG  YYIL V+    GGL++G
Sbjct: 2   RNFALLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLSIG 61

Query: 63  NLQS-EICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVWK 122
            +   E CP+N++ E ++ ++G P TF P+NPKKGVVRVSTDLN+QF+A+T C  STVWK
Sbjct: 62  GIYGYEKCPINILPESYDYLHGLPATFSPINPKKGVVRVSTDLNIQFEANTRCGISTVWK 121

Query: 123 LDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIG 182
           + KFDE   Q+FVT+GG +GNPG ET++NWFK+EK+GK+YKLV+CPTVC +CKV+C+D+G
Sbjct: 122 VGKFDEYLKQYFVTMGGMKGNPGRETIENWFKVEKYGKNYKLVYCPTVCKYCKVVCKDVG 181

Query: 183 IFFKNGKRALALSDTPFPVMFKKV 206
           +F+KNG+R +AL+D PFPVMFKKV
Sbjct: 182 LFYKNGRRVIALNDAPFPVMFKKV 205

[Close]

BLAST of MELO3C002175.2 vs. TrEMBL
Match: tr|A0A2P5BXZ8|A0A2P5BXZ8_9ROSA (Proteinase inhibitor I3, Kunitz legume OS=Trema orientalis OX=63057 GN=TorRG33x02_304910 PE=4 SV=1)

HSP 1 Score: 287.0 bits (733), Expect = 3.4e-74
Identity = 134/195 (68.72%), Postives = 159/195 (81.54%), Query Frame = 0


Query: 12  FILLASTELRFST-ADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLGNLQSEICP 71
           F+LL  T    S+ ADA+P AVLDI GKKL+ GVNY+ILPV RGRGGGLTL   +++ CP
Sbjct: 11  FLLLYFTAKHASSLADAAPAAVLDISGKKLQKGVNYHILPVIRGRGGGLTLAATRNKSCP 70

Query: 72  VNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVWKLDKFDESTG 131
           ++VVQEQFE+ +G P TF PVNP++ VVR+STDLN++F A+TICV STVWKLD F+E  G
Sbjct: 71  LDVVQEQFEVSSGLPLTFSPVNPRESVVRLSTDLNIKFSAATICVQSTVWKLDNFNELLG 130

Query: 132 QWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIGIFFKNGKRA 191
           QWFVT GG  GNPG ET+ NWFKIEKH  DYKLVFCPTVCNFCKV+CRD+GIF K+G R+
Sbjct: 131 QWFVTTGGVEGNPGHETISNWFKIEKHNDDYKLVFCPTVCNFCKVLCRDVGIFIKDGIRS 190

Query: 192 LALSDTPFPVMFKKV 206
           LALSD PF VMFK+V
Sbjct: 191 LALSDQPFIVMFKRV 205

The following BLAST results are available for this feature:

BLAST of MELO3C002175.2 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008437058.1	3.8e-117	100.00	PREDICTED: miraculin-like [Cucumis melo]	[more]
XP_004137534.1	8.5e-109	94.63	PREDICTED: miraculin-like [Cucumis sativus] >KGN64217.1 Tumor-related protein [C...	[more]
XP_022923703.1	2.6e-97	84.73	kunitz trypsin inhibitor 2 [Cucurbita moschata] >XP_023001808.1 kunitz trypsin i...	[more]
XP_023520252.1	5.7e-97	84.73	kunitz trypsin inhibitor 2 [Cucurbita pepo subsp. pepo]	[more]
XP_022137472.1	1.2e-91	77.83	kunitz trypsin inhibitor 2 [Momordica charantia]	[more]

BLAST of MELO3C002175.2 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G17860.1	2.8e-61	56.12	Kunitz family trypsin and protease inhibitor protein	[more]
AT1G73260.1	6.2e-37	43.88	kunitz trypsin inhibitor 1	[more]
AT1G73325.1	6.0e-24	36.32	Kunitz family trypsin and protease inhibitor protein	[more]

BLAST of MELO3C002175.2 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q9LMU2\|KTI2_ARATH	5.0e-60	56.12	Kunitz trypsin inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=KTI2 PE=2 SV=1	[more]
sp\|P13087\|MIRA_SYNDU	1.3e-55	54.85	Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3	[more]
sp\|P32765\|ASP_THECC	2.3e-41	47.34	21 kDa seed protein OS=Theobroma cacao OX=3641 GN=ASP PE=2 SV=1	[more]
sp\|Q8RXD5\|KTI1_ARATH	1.1e-35	43.88	Kunitz trypsin inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=KTI1 PE=2 SV=1	[more]
sp\|P29421\|IAAS_ORYSJ	8.8e-25	37.76	Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica OX=39947 GN=R...	[more]

BLAST of MELO3C002175.2 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S3ASR3\|A0A1S3ASR3_CUCME	2.5e-117	100.00	miraculin-like OS=Cucumis melo OX=3656 GN=LOC103482596 PE=4 SV=1	[more]
tr\|A0A0A0LTW2\|A0A0A0LTW2_CUCSA	5.6e-109	94.63	Tumor-related protein OS=Cucumis sativus OX=3659 GN=Csa_1G043200 PE=4 SV=1	[more]
tr\|A0A1S3AST3\|A0A1S3AST3_CUCME	8.2e-76	62.75	miraculin OS=Cucumis melo OX=3656 GN=LOC103482610 PE=4 SV=1	[more]
tr\|A0A0A0LT81\|A0A0A0LT81_CUCSA	1.8e-75	61.76	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G043220 PE=4 SV=1	[more]
tr\|A0A2P5BXZ8\|A0A2P5BXZ8_9ROSA	3.4e-74	68.72	Proteinase inhibitor I3, Kunitz legume OS=Trema orientalis OX=63057 GN=TorRG33x0...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004866	endopeptidase inhibitor activity

Vocabulary: **INTERPRO**
Term	Definition
IPR011065	Kunitz_inhibitor_STI-like_sf
IPR002160	Prot_inh_Kunz-lg

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004866	endopeptidase inhibitor activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MELO3C002175.2.1	MELO3C002175.2.1	mRNA

InterPro

Analysis Name: InterPro Annotations of melon v3.6.1
Date Performed: 2018-09-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002160	Proteinase inhibitor I3, Kunitz legume	PRINTS	PR00291	KUNITZINHBTR	coord: 151..170 score: 25.29 coord: 174..203 score: 25.03 coord: 31..60 score: 46.34 coord: 69..89 score: 31.19
IPR002160	Proteinase inhibitor I3, Kunitz legume	SMART	SM00452	kul_2	coord: 31..205 e-value: 1.2E-72 score: 257.4
IPR002160	Proteinase inhibitor I3, Kunitz legume	PFAM	PF00197	Kunitz_legume	coord: 32..204 e-value: 2.8E-60 score: 203.2
IPR002160	Proteinase inhibitor I3, Kunitz legume	PANTHER	PTHR33107	FAMILY NOT NAMED	coord: 3..205
IPR002160	Proteinase inhibitor I3, Kunitz legume	PROSITE	PS00283	SOYBEAN_KUNITZ	coord: 32..48
IPR002160	Proteinase inhibitor I3, Kunitz legume	CDD	cd00178	STI	coord: 31..204 e-value: 1.94587E-55 score: 175.223
None	No IPR available	GENE3D	G3DSA:2.80.10.50		coord: 29..205 e-value: 3.4E-72 score: 244.0
IPR011065	Kunitz inhibitor STI-like superfamily	SUPERFAMILY	SSF50386	STI-like	coord: 28..205

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
MELO3C002175.2	Wax gourd	medwgoB175
MELO3C002175.2	Cucumber (Gy14) v2	cgybmedB020
MELO3C002175.2	Cucumber (Gy14) v1	cgymedB182
MELO3C002175.2	Cucurbita maxima (Rimu)	cmamedB214
MELO3C002175.2	Cucurbita maxima (Rimu)	cmamedB424
MELO3C002175.2	Cucurbita moschata (Rifu)	cmomedB202
MELO3C002175.2	Cucurbita moschata (Rifu)	cmomedB408
MELO3C002175.2	Cucurbita pepo (Zucchini)	cpemedB031
MELO3C002175.2	Cucurbita pepo (Zucchini)	cpemedB560
MELO3C002175.2	Cucumber (Chinese Long) v2	cumedB022
MELO3C002175.2	Wild cucumber (PI 183967)	cpimedB023
MELO3C002175.2	Bottle gourd (USVL1VR-Ls)	lsimedB189
MELO3C002175.2	Watermelon (Charleston Gray)	medwcgB125
MELO3C002175.2	Watermelon (97103) v1	medwmB163
MELO3C002175.2	Silver-seed gourd	carmedB0397
MELO3C002175.2	Silver-seed gourd	carmedB0504
MELO3C002175.2	Cucumber (Chinese Long) v3	cucmedB022
MELO3C002175.2	Watermelon (97103) v2	medwmbB125