MELO3C002175 (gene) Melon (DHL92) v3.5.1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	MELO3C002175
Type	gene
Organism	Cucumis melo (Melon (DHL92) v3.5.1)
Description	Kunitz trypsin inhibitor
Location	chr12 : 24661114 .. 24661982 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TAAGCATCCAAACAAAACAAAGTCCACATTCAAAAGAAAAATGACGAAGAATTTCGGAATATTCTATTTTATTTTCATTCTCCTTGCCTCAACCGAGCTCCGCTTCTCCACAGCCGACGCTTCACCGGAGGCCGTCCTCGATATCGACGGCAAGAAGCTCCGAGCCGGCGTCAACTACTACATCCTCCCTGTTTTCCGCGGCAGAGGCGGCGGCCTAACTCTAGGCAACCTCCAATCGGAGATATGTCCAGTCAACGTCGTTCAAGAACAATTCGAATTAATGAACGGATTTCCAACAACATTCCATCCTGTAAACCCTAAAAAAGGAGTGGTTCGAGTTTCAACCGATTTGAATGTGCAATTCGATGCGAGTACGATCTGCGTGACATCGACGGTGTGGAAATTGGACAAATTCGACGAATCGACGGGACAATGGTTCGTGACGATCGGCGGAAGCAGGGGAAATCCGGGAGTGGAGACGGTGGATAATTGGTTCAAAATTGAGAAGCACGGTAAGGATTACAAATTGGTGTTCTGTCCGACTGTTTGTAATTTCTGTAAAGTTATGTGTAGAGATATTGGAATCTTCTTCAAGAATGGAAAAAGGGCTTTGGCTTTGAGCGATACGCCATTCCCTGTTATGTTCAAGAAAGTTTAATTGAAGAAAATTCAAAAATTAAAGTGAATGAGTTTATGAAGTAATTTGTGTTTAATTCATGTATAAGAGTTTTATCATAATTAAATAAAAGATATGGTTATTGTTTGTTTATTATTATTAGAAGAATAAAATACTTTATTTGATTATTTTCGTGTAAGCTGTTTTCACCTCAGTTTCTCAAAATCAAAGTACGTCTTATTTTCGTACTTAT

mRNA sequence

TAAGCATCCAAACAAAACAAAGTCCACATTCAAAAGAAAAATGACGAAGAATTTCGGAATATTCTATTTTATTTTCATTCTCCTTGCCTCAACCGAGCTCCGCTTCTCCACAGCCGACGCTTCACCGGAGGCCGTCCTCGATATCGACGGCAAGAAGCTCCGAGCCGGCGTCAACTACTACATCCTCCCTGTTTTCCGCGGCAGAGGCGGCGGCCTAACTCTAGGCAACCTCCAATCGGAGATATGTCCAGTCAACGTCGTTCAAGAACAATTCGAATTAATGAACGGATTTCCAACAACATTCCATCCTGTAAACCCTAAAAAAGGAGTGGTTCGAGTTTCAACCGATTTGAATGTGCAATTCGATGCGAGTACGATCTGCGTGACATCGACGGTGTGGAAATTGGACAAATTCGACGAATCGACGGGACAATGGTTCGTGACGATCGGCGGAAGCAGGGGAAATCCGGGAGTGGAGACGGTGGATAATTGGTTCAAAATTGAGAAGCACGGTAAGGATTACAAATTGGTGTTCTGTCCGACTGTTTGTAATTTCTGTAAAGTTATGTGTAGAGATATTGGAATCTTCTTCAAGAATGGAAAAAGGGCTTTGGCTTTGAGCGATACGCCATTCCCTGTTATGTTCAAGAAAGTTTAATTGAAGAAAATTCAAAAATTAAAGTGAATGAGTTTATGAAGTAATTTGTGTTTAATTCATGTATAAGAGTTTTATCATAATTAAATAAAAGATATGGTTATTGTTTGTTTATTATTATTAGAAGAATAAAATACTTTATTTGATTATTTTCGTGTAAGCTGTTTTCACCTCAGTTTCTCAAAATCAAAGTACGTCTTATTTTCGTACTTAT

Coding sequence (CDS)

ATGACGAAGAATTTCGGAATATTCTATTTTATTTTCATTCTCCTTGCCTCAACCGAGCTCCGCTTCTCCACAGCCGACGCTTCACCGGAGGCCGTCCTCGATATCGACGGCAAGAAGCTCCGAGCCGGCGTCAACTACTACATCCTCCCTGTTTTCCGCGGCAGAGGCGGCGGCCTAACTCTAGGCAACCTCCAATCGGAGATATGTCCAGTCAACGTCGTTCAAGAACAATTCGAATTAATGAACGGATTTCCAACAACATTCCATCCTGTAAACCCTAAAAAAGGAGTGGTTCGAGTTTCAACCGATTTGAATGTGCAATTCGATGCGAGTACGATCTGCGTGACATCGACGGTGTGGAAATTGGACAAATTCGACGAATCGACGGGACAATGGTTCGTGACGATCGGCGGAAGCAGGGGAAATCCGGGAGTGGAGACGGTGGATAATTGGTTCAAAATTGAGAAGCACGGTAAGGATTACAAATTGGTGTTCTGTCCGACTGTTTGTAATTTCTGTAAAGTTATGTGTAGAGATATTGGAATCTTCTTCAAGAATGGAAAAAGGGCTTTGGCTTTGAGCGATACGCCATTCCCTGTTATGTTCAAGAAAGTTTAA

Protein sequence

MTKNFGIFYFIFILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLGNLQSEICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVWKLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIGIFFKNGKRALALSDTPFPVMFKKV*

Homology

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BLAST of MELO3C002175 vs. Swiss-Prot
Match: MIRA_SYNDU (Miraculin OS=Synsepalum dulcificum PE=1 SV=3)

HSP 1 Score: 214.2 bits (544), Expect = 1.4e-54
Identity = 113/206 (54.85%), Postives = 137/206 (66.50%), Query Frame = 1


Query: 8   FYFIFILLAST-ELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLGNLQS 67
           F+F+  LLA+      S AD++P  VLDIDG+KLR G NYYI+PV R  GGGLT+     
Sbjct: 11  FFFVSALLAAAANPLLSAADSAPNPVLDIDGEKLRTGTNYYIVPVLRDHGGGLTVSATTP 70

Query: 68  E---ICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTIC--VTSTVWK 127
               +CP  VVQ + E+ +  P  F P NPK+ VVRVSTDLN+ F A   C   +STVW+
Sbjct: 71  NGTFVCPPRVVQTRKEVDHDRPLAFFPENPKEDVVRVSTDLNINFSAFMPCRWTSSTVWR 130

Query: 128 LDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKH--GKDYKLVFCPTVCNFCKVMCRD 187
           LDK+DESTGQ+FVTIGG +GNPG ET+ +WFKIE+      YKLVFCPTVC  CKV C D
Sbjct: 131 LDKYDESTGQYFVTIGGVKGNPGPETISSWFKIEEFCGSGFYKLVFCPTVCGSCKVKCGD 190

Query: 188 IGIFF-KNGKRALALSDTPFPVMFKK 205
           +GI+  + G+R LALSD PF   F K
Sbjct: 191 VGIYIDQKGRRRLALSDKPFAFEFNK 216

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BLAST of MELO3C002175 vs. Swiss-Prot
Match: ASP_THECC (21 kDa seed protein OS=Theobroma cacao GN=ASP PE=2 SV=1)

HSP 1 Score: 164.9 bits (416), Expect = 9.6e-40
Identity = 89/188 (47.34%), Postives = 113/188 (60.11%), Query Frame = 1


Query: 22  FSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGG-LTLGNLQSEICPVNVVQEQFEL 81
           F  A+A+   VLD DG +L+ GV YY+L    G GGG L LG    + CP  VVQ + +L
Sbjct: 21  FGVANAANSPVLDTDGDELQTGVQYYVLSSISGAGGGGLALGRATGQSCPEIVVQRRSDL 80

Query: 82  MNGFPTTFHPVNPKKGVVRVSTDLNVQFDA--STICVTSTVWKLDKFDESTGQWFVTIGG 141
            NG P  F   + K  VVRVSTD+N++F      +C TSTVW+LD +D S G+W+VT  G
Sbjct: 81  DNGTPVIFSNADSKDDVVRVSTDVNIEFVPIRDRLCSTSTVWRLDNYDNSAGKWWVTTDG 140

Query: 142 SRGNPGVETVDNWFKIEKHG-KDYKLVFCPTVCNFCKVMCRDIGIFF-KNGKRALALSDT 201
            +G PG  T+ +WFKIEK G   YK  FCP+VC+ C  +C DIG     +G+  LALSD 
Sbjct: 141 VKGEPGPNTLCSWFKIEKAGVLGYKFRFCPSVCDSCTTLCSDIGRHSDDDGQIRLALSDN 200

Query: 202 PFPVMFKK 205
            +  MFKK
Sbjct: 201 EWAWMFKK 208

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BLAST of MELO3C002175 vs. Swiss-Prot
Match: IAAS_ORYSJ (Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica GN=RASI PE=1 SV=2)

HSP 1 Score: 110.5 bits (275), Expect = 2.2e-23
Identity = 74/196 (37.76%), Postives = 101/196 (51.53%), Query Frame = 1


Query: 13  ILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLGNLQSEICPVN 72
           ILL+   + FS + A+P  V D +G +L A  +YY+LP   G GGGLT+       CP+ 
Sbjct: 9   ILLSLLAISFSCS-AAPPPVYDTEGHELSADGSYYVLPASPGHGGGLTMAPRVLP-CPLL 68

Query: 73  VVQEQFELMNGFPTTFHP----VNPKKGVVRVSTDLNVQFDASTICVTSTVWKLDKFDES 132
           V QE  E   GFP  F P      P+   +RVSTD+ ++F+A+TICV ST W +   +  
Sbjct: 69  VAQETDERRKGFPVRFTPWGGAAAPEDRTIRVSTDVRIRFNAATICVQSTEWHVGD-EPL 128

Query: 133 TGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIGIFFKNGK 192
           TG   V  G   G P     +N F++EK+G  YKLV        C+  C+D+G+     +
Sbjct: 129 TGARRVVTGPLIG-PSPSGRENAFRVEKYGGGYKLV-------SCRDSCQDLGVSRDGAR 188

Query: 193 RALALSDTPFPVMFKK 205
             L  S  P  V+FKK
Sbjct: 189 AWLGASQPPHVVVFKK 193

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BLAST of MELO3C002175 vs. Swiss-Prot
Match: LSPI_CARPA (Latex serine proteinase inhibitor OS=Carica papaya PE=1 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 2.8e-23
Identity = 70/183 (38.25%), Postives = 103/183 (56.28%), Query Frame = 1


Query: 28  SPEAVLDIDGKKLRAGVNYYILPVFRGRGGG-LTL-GNLQSEICPVNVVQEQFELMNGFP 87
           +P+ ++DIDGK +  GV+Y+++    G GGG LT+ G    + CP++VVQ+ F+  NG P
Sbjct: 2   APKPIVDIDGKPVLYGVDYFVVSAIWGAGGGGLTVYGPGNKKKCPLSVVQDPFD--NGEP 61

Query: 88  TTFHPV-NPKKGVVRVSTDLNVQFDASTICVTSTVWKLDKFDESTGQWFVTIGGSRGNPG 147
             F  + N K  +V  S DLNV+F+ +  C  +T WK+D+F    G W VT+GG +G  G
Sbjct: 62  IIFSAIKNVKDNIVFESVDLNVKFNITINCNETTAWKVDRFPGVIG-WTVTLGGEKGYHG 121

Query: 148 VETVDNWFKIEKHGK--DYKLVFCPTVCNFCKVMCRDIGIFF-KNGKRALALSDTPFPVM 205
            E+  + FKI+K G    YK  FCP+      + C ++ IFF K   R L L++     +
Sbjct: 122 FESTHSMFKIKKAGLPFSYKFHFCPSYPRTRLIPCNNVDIFFDKYRIRRLILTNDAKEFV 181

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BLAST of MELO3C002175 vs. Swiss-Prot
Match: PIP21_PEA (Kunitz-type trypsin inhibitor-like 1 protein OS=Pisum sativum GN=PIP20-1 PE=1 SV=2)

HSP 1 Score: 107.8 bits (268), Expect = 1.4e-22
Identity = 72/203 (35.47%), Postives = 98/203 (48.28%), Query Frame = 1


Query: 9   YFIFILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRG-RGGGLTLGNLQSE 68
           +F+F+ + +  L FS  D   E VLD +G  +  GV Y+ILP  RG  GGG+ LG     
Sbjct: 11  FFLFVFITNLSLAFSNEDV--EQVLDFNGNPIFPGVQYFILPAIRGPAGGGVRLGRTGDL 70

Query: 69  ICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVWKLDKFDE 128
            CPV V+Q++ E+ NG P  F       G++   T + +++     C  S+ W L   D 
Sbjct: 71  TCPVTVLQDRQEVKNGLPVKFVIPEISPGIIFTGTPIEIEYTKKPNCAKSSKW-LVFVDN 130

Query: 129 STGQWFVTIGGSRGNPGVETVDNWFKIEKH--GKDYKLVFC----PTVCNFCKVMCRDIG 188
              +  V IGG    PGV+T+   FKIEKH  G  YKL FC    PT        C D+G
Sbjct: 131 VIQKACVGIGGPENYPGVQTLSGLFKIEKHESGFGYKLGFCVKGSPT--------CLDVG 190

Query: 189 IF--FKNGKRALALSDTPFPVMF 203
            F   ++G+R        F V+F
Sbjct: 191 RFDNDEDGRRLNLTEHESFQVVF 202

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BLAST of MELO3C002175 vs. TrEMBL
Match: A0A0A0LTW2_CUCSA (Tumor-related protein OS=Cucumis sativus GN=Csa_1G043200 PE=4 SV=1)

HSP 1 Score: 397.9 bits (1021), Expect = 7.5e-108
Identity = 193/205 (94.15%), Postives = 200/205 (97.56%), Query Frame = 1


Query: 3   KNFGI-FYFIFILLASTE-LRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLT 62
           KNFGI FYF+FILLAST+ +RFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLT
Sbjct: 2   KNFGILFYFLFILLASTQLIRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLT 61

Query: 63  LGNLQSEICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVW 122
           LGNLQSE CP+NVVQEQ E+MNGFPTTFHPVNPKKGVVRVSTDLNVQF+ASTICVTSTVW
Sbjct: 62  LGNLQSEKCPLNVVQEQLEVMNGFPTTFHPVNPKKGVVRVSTDLNVQFEASTICVTSTVW 121

Query: 123 KLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDI 182
           KLDKFDESTGQW VTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDI
Sbjct: 122 KLDKFDESTGQWLVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDI 181

Query: 183 GIFFKNGKRALALSDTPFPVMFKKV 206
           GIFFKNG+RALALSDTPFPVMFKKV
Sbjct: 182 GIFFKNGERALALSDTPFPVMFKKV 206

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BLAST of MELO3C002175 vs. TrEMBL
Match: A0A0A0LT81_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G043220 PE=4 SV=1)

HSP 1 Score: 287.3 bits (734), Expect = 1.4e-74
Identity = 126/204 (61.76%), Postives = 168/204 (82.35%), Query Frame = 1


Query: 3   KNFGIFYFIFILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLG 62
           +NF +  F+FI++AS+E+RF  ADASP+AVLD DGKKLRAG  YYIL V+    GGL++G
Sbjct: 2   RNFALLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLSIG 61

Query: 63  NLQS-EICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVWK 122
            +   E CP+N++ E ++ ++G P TF P+NPKKGVVRVSTDLN+QF+A+T C  STVWK
Sbjct: 62  GIYGYEKCPINILPESYDYLHGLPATFSPINPKKGVVRVSTDLNIQFEANTRCGISTVWK 121

Query: 123 LDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIG 182
           + KFDE   Q+FVT+GG +GNPG ET++NWFK+EK+GK+YKLV+CPTVC +CKV+C+D+G
Sbjct: 122 VGKFDEYLKQYFVTMGGMKGNPGRETIENWFKVEKYGKNYKLVYCPTVCKYCKVVCKDVG 181

Query: 183 IFFKNGKRALALSDTPFPVMFKKV 206
           +F+KNG+R +AL+D PFPVMFKKV
Sbjct: 182 LFYKNGRRVIALNDAPFPVMFKKV 205

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BLAST of MELO3C002175 vs. TrEMBL
Match: M5X024_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011496mg PE=4 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 8.7e-72
Identity = 136/198 (68.69%), Postives = 155/198 (78.28%), Query Frame = 1


Query: 10  FIFILLA-STELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLGNLQSEI 69
           F F+L A S +LR   ADA+P  VLDI G KL+ GV+YYILPV RGRGGGLTL +  ++ 
Sbjct: 11  FCFLLFAFSAKLRSVAADAAPSPVLDITGNKLQTGVDYYILPVIRGRGGGLTLASTSNKT 70

Query: 70  -CPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVWKLDKFDE 129
            CP++VVQEQ E+ NG P  F PVN  KGVVRVSTDLN++F A+TICV STVWKL KFDE
Sbjct: 71  SCPLDVVQEQNEVSNGLPLKFSPVNVTKGVVRVSTDLNIKFSATTICVQSTVWKLGKFDE 130

Query: 130 STGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIGIFFKNG 189
            TGQWFVT GG  GNPG +T  NWFKIEK G DYKLVFCPTVCNFCKV+C D+GIFF++G
Sbjct: 131 QTGQWFVTSGGVEGNPGRQTTSNWFKIEKFGDDYKLVFCPTVCNFCKVICGDVGIFFQDG 190

Query: 190 KRALALSDTPFPVMFKKV 206
           KR LALSD PF  MFKKV
Sbjct: 191 KRRLALSDVPFRAMFKKV 208

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BLAST of MELO3C002175 vs. TrEMBL
Match: A5AXN3_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_027379 PE=4 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 1.3e-70
Identity = 132/196 (67.35%), Postives = 159/196 (81.12%), Query Frame = 1


Query: 10  FIFILLASTELRFSTA-DASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLGNLQSEI 69
           F  +L+A     FS A +++P+ VLD +GKKLR+GV+YYILPVFRGRGGGLTL +  +E 
Sbjct: 8   FSLLLIALAVKPFSVAAESAPDPVLDTEGKKLRSGVDYYILPVFRGRGGGLTLASTGNES 67

Query: 70  CPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVWKLDKFDES 129
           CP++VVQEQ E+ NG P TF PVNPKKGV+RVSTD N++F ASTICV ST+WKL+ +DES
Sbjct: 68  CPLDVVQEQQEVSNGLPLTFTPVNPKKGVIRVSTDHNIKFSASTICVQSTLWKLE-YDES 127

Query: 130 TGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIGIFFKNGK 189
           +GQWFVT GG  GNPG ET+DNWFKIEK+  DYKLVFCPTVC+FCK +C DIGI+ +NG 
Sbjct: 128 SGQWFVTTGGVEGNPGRETLDNWFKIEKYEDDYKLVFCPTVCDFCKPVCGDIGIYIQNGY 187

Query: 190 RALALSDTPFPVMFKK 205
           R LALSD PF VMFKK
Sbjct: 188 RRLALSDVPFKVMFKK 202

[Close]

BLAST of MELO3C002175 vs. TrEMBL
Match: A0A061EZK2_THECC (Kunitz family trypsin and protease inhibitor protein OS=Theobroma cacao GN=TCM_025233 PE=4 SV=1)

HSP 1 Score: 273.9 bits (699), Expect = 1.6e-70
Identity = 126/180 (70.00%), Postives = 147/180 (81.67%), Query Frame = 1


Query: 25  ADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLGNLQSEICPVNVVQEQFELMNGF 84
           A+A+P+ VLDI GKKLR G +YYILPVFRGRGGGLTL +  +E CP++VVQEQ E+ +G 
Sbjct: 15  ANAAPDPVLDISGKKLRTGTDYYILPVFRGRGGGLTLASTGNESCPLDVVQEQLEVSDGL 74

Query: 85  PTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVWKLDKFDESTGQWFVTIGGSRGNPG 144
           P TF PVN KKGVVRVSTD N++F A+TICV  T+WKLD FD+ST QWFVT GG  GNPG
Sbjct: 75  PVTFSPVNIKKGVVRVSTDQNIKFSAATICVQPTLWKLDSFDDSTRQWFVTTGGVEGNPG 134

Query: 145 VETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIGIFFKNGKRALALSDTPFPVMFKK 204
            ET+DNWFKIEK+  DYKLVFCPTVC+FCKVMCRD+G+F   G R LALSD PF VMFK+
Sbjct: 135 RETIDNWFKIEKYEDDYKLVFCPTVCDFCKVMCRDVGVFIDGGVRRLALSDVPFKVMFKR 194

[Close]

BLAST of MELO3C002175 vs. TAIR10
Match: AT1G17860.1 (AT1G17860.1 Kunitz family trypsin and protease inhibitor protein)

HSP 1 Score: 227.6 bits (579), Expect = 6.8e-60
Identity = 110/196 (56.12%), Postives = 140/196 (71.43%), Query Frame = 1


Query: 10  FIFILLAS-TELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLGNLQSEI 69
           +IF+LLA     R  T +A+ E V DI+GK L  GVNYYILPV RGRGGGLT+ NL++E 
Sbjct: 6   YIFLLLAVFISHRGVTTEAAVEPVKDINGKSLLTGVNYYILPVIRGRGGGLTMSNLKTET 65

Query: 70  CPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVWKLDKFDES 129
           CP +V+Q+QFE+  G P  F P + K   + VSTD+N++F  ++I      W+L  FDE+
Sbjct: 66  CPTSVIQDQFEVSQGLPVKFSPYD-KSRTIPVSTDVNIKFSPTSI------WELANFDET 125

Query: 130 TGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIGIFFKNGK 189
           T QWF++  G  GNPG +TVDNWFKI+K  KDYK+ FCPTVCNFCKV+CRD+G+F ++GK
Sbjct: 126 TKQWFISTCGVEGNPGQKTVDNWFKIDKFEKDYKIRFCPTVCNFCKVICRDVGVFVQDGK 185

Query: 190 RALALSDTPFPVMFKK 205
           R LALSD P  VMFK+
Sbjct: 186 RRLALSDVPLKVMFKR 194

[Close]

BLAST of MELO3C002175 vs. TAIR10
Match: AT1G73260.1 (AT1G73260.1 kunitz trypsin inhibitor 1)

HSP 1 Score: 147.1 bits (370), Expect = 1.2e-35
Identity = 86/196 (43.88%), Postives = 113/196 (57.65%), Query Frame = 1


Query: 12  FILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLGNLQSEICPV 71
           +++LA T +  S A     AV+DIDG  +    +YY+LPV RGRGGGLTL     + CP 
Sbjct: 13  YLVLALTAVLASNAYG---AVVDIDGNAM-FHESYYVLPVIRGRGGGLTLAGRGGQPCPY 72

Query: 72  NVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFD-ASTICVTSTVWKLDKFDESTG 131
           ++VQE  E+  G P  F     K   V  S +LN++ D  +TIC+ ST W++ +FD    
Sbjct: 73  DIVQESSEVDEGIPVKFSNWRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHERK 132

Query: 132 QWFVTIGGSRGNPGVETVDNWFKIEKHGKD-YKLVFCPTVCNFCKVMCRDIGIFFKN-GK 191
           Q+FV  G      G +++ ++FKIEK G+D YK VFCP  C+     C D+GIF    G 
Sbjct: 133 QYFVVAGPKPEGFGQDSLKSFFKIEKSGEDAYKFVFCPRTCDSGNPKCSDVGIFIDELGV 192

Query: 192 RALALSDTPFPVMFKK 205
           R LALSD PF VMFKK
Sbjct: 193 RRLALSDKPFLVMFKK 204

[Close]

BLAST of MELO3C002175 vs. TAIR10
Match: AT1G73325.1 (AT1G73325.1 Kunitz family trypsin and protease inhibitor protein)

HSP 1 Score: 103.2 bits (256), Expect = 1.9e-22
Identity = 69/190 (36.32%), Postives = 105/190 (55.26%), Query Frame = 1


Query: 23  STADASP-EAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLGNLQ---SEIC-PVNVVQEQ 82
           S ADA+P + VLDI G  +++ V YYI+P   G GGGL   N      ++C  +++VQ  
Sbjct: 22  SAADATPSQVVLDIAGHPVQSNVQYYIIPAKIGTGGGLIPSNRNLSTQDLCLNLDIVQSS 81

Query: 83  FELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDAST-ICVTSTVWKLDKFDESTGQWFVTI 142
              ++G P TF P+N K   V++S  LN++FD++  +C  S VW++D       + FV+I
Sbjct: 82  SPFVSGLPVTFSPLNTKVKHVQLSASLNLEFDSTVWLCPDSKVWRID-HSVQLRKSFVSI 141

Query: 143 GGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIGI-FFKNGKRALALS- 202
           GG +G       ++WF+I++ G  YKL++CP       V C ++ +    +G R L LS 
Sbjct: 142 GGQKGKG-----NSWFQIQEDGDAYKLMYCPI---SSIVACINVSLEIDDHGVRRLVLST 201

Query: 203 DTPFPVMFKK 205
           D  F V F+K
Sbjct: 202 DQSFVVKFQK 202

[Close]

BLAST of MELO3C002175 vs. NCBI nr
Match: gi|659066983|ref|XP_008437058.1| (PREDICTED: miraculin-like [Cucumis melo])

HSP 1 Score: 426.4 bits (1095), Expect = 2.8e-116
Identity = 205/205 (100.00%), Postives = 205/205 (100.00%), Query Frame = 1


Query: 1   MTKNFGIFYFIFILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLT 60
           MTKNFGIFYFIFILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLT
Sbjct: 1   MTKNFGIFYFIFILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLT 60

Query: 61  LGNLQSEICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVW 120
           LGNLQSEICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVW
Sbjct: 61  LGNLQSEICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVW 120

Query: 121 KLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDI 180
           KLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDI
Sbjct: 121 KLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDI 180

Query: 181 GIFFKNGKRALALSDTPFPVMFKKV 206
           GIFFKNGKRALALSDTPFPVMFKKV
Sbjct: 181 GIFFKNGKRALALSDTPFPVMFKKV 205

[Close]

BLAST of MELO3C002175 vs. NCBI nr
Match: gi|449439521|ref|XP_004137534.1| (PREDICTED: miraculin-like [Cucumis sativus])

HSP 1 Score: 397.9 bits (1021), Expect = 1.1e-107
Identity = 193/205 (94.15%), Postives = 200/205 (97.56%), Query Frame = 1


Query: 3   KNFGI-FYFIFILLASTE-LRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLT 62
           KNFGI FYF+FILLAST+ +RFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLT
Sbjct: 2   KNFGILFYFLFILLASTQLIRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLT 61

Query: 63  LGNLQSEICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVW 122
           LGNLQSE CP+NVVQEQ E+MNGFPTTFHPVNPKKGVVRVSTDLNVQF+ASTICVTSTVW
Sbjct: 62  LGNLQSEKCPLNVVQEQLEVMNGFPTTFHPVNPKKGVVRVSTDLNVQFEASTICVTSTVW 121

Query: 123 KLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDI 182
           KLDKFDESTGQW VTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDI
Sbjct: 122 KLDKFDESTGQWLVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDI 181

Query: 183 GIFFKNGKRALALSDTPFPVMFKKV 206
           GIFFKNG+RALALSDTPFPVMFKKV
Sbjct: 182 GIFFKNGERALALSDTPFPVMFKKV 206

[Close]

BLAST of MELO3C002175 vs. NCBI nr
Match: gi|659066987|ref|XP_008437082.1| (PREDICTED: miraculin [Cucumis melo])

HSP 1 Score: 288.5 bits (737), Expect = 9.2e-75
Identity = 128/204 (62.75%), Postives = 166/204 (81.37%), Query Frame = 1


Query: 3   KNFGIFYFIFILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLG 62
           KNF +  F+FI++AS+E+RF  ADASP+AVLD DGKKLRAG  YYIL V+    GGL++G
Sbjct: 2   KNFALLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLSIG 61

Query: 63  NLQS-EICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVWK 122
            +   E CP+N++ E ++ ++G P TF PVNPKKGVVRVSTDLN++F+AST C  STVWK
Sbjct: 62  GIYGYEKCPINILPESYDYLDGLPATFSPVNPKKGVVRVSTDLNIEFEASTRCGISTVWK 121

Query: 123 LDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIG 182
           + KFD+   Q+FVT+GG++GNPG ET+ NWFK+EKHGK+YK V+CPTVC +CKVMC+D+G
Sbjct: 122 VGKFDQYLKQYFVTMGGTKGNPGRETIGNWFKVEKHGKNYKFVYCPTVCKYCKVMCKDVG 181

Query: 183 IFFKNGKRALALSDTPFPVMFKKV 206
           +F+KNG+R  AL+D PFPVMFKKV
Sbjct: 182 LFYKNGRRIFALNDAPFPVMFKKV 205

[Close]

BLAST of MELO3C002175 vs. NCBI nr
Match: gi|449439731|ref|XP_004137639.1| (PREDICTED: miraculin [Cucumis sativus])

HSP 1 Score: 287.3 bits (734), Expect = 2.1e-74
Identity = 126/204 (61.76%), Postives = 168/204 (82.35%), Query Frame = 1


Query: 3   KNFGIFYFIFILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLG 62
           +NF +  F+FI++AS+E+RF  ADASP+AVLD DGKKLRAG  YYIL V+    GGL++G
Sbjct: 2   RNFALLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLSIG 61

Query: 63  NLQS-EICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVWK 122
            +   E CP+N++ E ++ ++G P TF P+NPKKGVVRVSTDLN+QF+A+T C  STVWK
Sbjct: 62  GIYGYEKCPINILPESYDYLHGLPATFSPINPKKGVVRVSTDLNIQFEANTRCGISTVWK 121

Query: 123 LDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIG 182
           + KFDE   Q+FVT+GG +GNPG ET++NWFK+EK+GK+YKLV+CPTVC +CKV+C+D+G
Sbjct: 122 VGKFDEYLKQYFVTMGGMKGNPGRETIENWFKVEKYGKNYKLVYCPTVCKYCKVVCKDVG 181

Query: 183 IFFKNGKRALALSDTPFPVMFKKV 206
           +F+KNG+R +AL+D PFPVMFKKV
Sbjct: 182 LFYKNGRRVIALNDAPFPVMFKKV 205

[Close]

BLAST of MELO3C002175 vs. NCBI nr
Match: gi|595860796|ref|XP_007211211.1| (hypothetical protein PRUPE_ppa011496mg [Prunus persica])

HSP 1 Score: 278.1 bits (710), Expect = 1.2e-71
Identity = 136/198 (68.69%), Postives = 155/198 (78.28%), Query Frame = 1


Query: 10  FIFILLA-STELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLGNLQSEI 69
           F F+L A S +LR   ADA+P  VLDI G KL+ GV+YYILPV RGRGGGLTL +  ++ 
Sbjct: 11  FCFLLFAFSAKLRSVAADAAPSPVLDITGNKLQTGVDYYILPVIRGRGGGLTLASTSNKT 70

Query: 70  -CPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVWKLDKFDE 129
            CP++VVQEQ E+ NG P  F PVN  KGVVRVSTDLN++F A+TICV STVWKL KFDE
Sbjct: 71  SCPLDVVQEQNEVSNGLPLKFSPVNVTKGVVRVSTDLNIKFSATTICVQSTVWKLGKFDE 130

Query: 130 STGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIGIFFKNG 189
            TGQWFVT GG  GNPG +T  NWFKIEK G DYKLVFCPTVCNFCKV+C D+GIFF++G
Sbjct: 131 QTGQWFVTSGGVEGNPGRQTTSNWFKIEKFGDDYKLVFCPTVCNFCKVICGDVGIFFQDG 190

Query: 190 KRALALSDTPFPVMFKKV 206
           KR LALSD PF  MFKKV
Sbjct: 191 KRRLALSDVPFRAMFKKV 208

The following BLAST results are available for this feature:

BLAST of MELO3C002175 vs. Swiss-Prot


Match Name	E-value	Identity	Description
MIRA_SYNDU	1.4e-54	54.85	Miraculin OS=Synsepalum dulcificum PE=1 SV=3	[more]
ASP_THECC	9.6e-40	47.34	21 kDa seed protein OS=Theobroma cacao GN=ASP PE=2 SV=1	[more]
IAAS_ORYSJ	2.2e-23	37.76	Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica GN=RASI PE=1 ...	[more]
LSPI_CARPA	2.8e-23	38.25	Latex serine proteinase inhibitor OS=Carica papaya PE=1 SV=1	[more]
PIP21_PEA	1.4e-22	35.47	Kunitz-type trypsin inhibitor-like 1 protein OS=Pisum sativum GN=PIP20-1 PE=1 SV...	[more]

BLAST of MELO3C002175 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LTW2_CUCSA	7.5e-108	94.15	Tumor-related protein OS=Cucumis sativus GN=Csa_1G043200 PE=4 SV=1	[more]
A0A0A0LT81_CUCSA	1.4e-74	61.76	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G043220 PE=4 SV=1	[more]
M5X024_PRUPE	8.7e-72	68.69	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011496mg PE=4 SV=1	[more]
A5AXN3_VITVI	1.3e-70	67.35	Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_027379 PE=4 SV=1	[more]
A0A061EZK2_THECC	1.6e-70	70.00	Kunitz family trypsin and protease inhibitor protein OS=Theobroma cacao GN=TCM_0...	[more]

BLAST of MELO3C002175 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G17860.1	6.8e-60	56.12	Kunitz family trypsin and protease inhibitor protein	[more]
AT1G73260.1	1.2e-35	43.88	kunitz trypsin inhibitor 1	[more]
AT1G73325.1	1.9e-22	36.32	Kunitz family trypsin and protease inhibitor protein	[more]

BLAST of MELO3C002175 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659066983\|ref\|XP_008437058.1\|	2.8e-116	100.00	PREDICTED: miraculin-like [Cucumis melo]	[more]
gi\|449439521\|ref\|XP_004137534.1\|	1.1e-107	94.15	PREDICTED: miraculin-like [Cucumis sativus]	[more]
gi\|659066987\|ref\|XP_008437082.1\|	9.2e-75	62.75	PREDICTED: miraculin [Cucumis melo]	[more]
gi\|449439731\|ref\|XP_004137639.1\|	2.1e-74	61.76	PREDICTED: miraculin [Cucumis sativus]	[more]
gi\|595860796\|ref\|XP_007211211.1\|	1.2e-71	68.69	hypothetical protein PRUPE_ppa011496mg [Prunus persica]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR002160	Prot_inh_Kunz-lg
IPR011065	Kunitz_inhibitor_STI-like_sf

Vocabulary: **Molecular Function**
Term	Definition
GO:0004866	endopeptidase inhibitor activity

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004866	endopeptidase inhibitor activity
molecular_function	GO:0008233	peptidase activity

Unigene

This gene is associated with the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
MU47870	melon EST collection version 4.0	transcribed_cluster

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MELO3C002175T1	MELO3C002175T1	mRNA

The following transcribed_cluster feature(s) are associated with this gene:


Feature Name	Unique Name	Type
MU47870	MU47870	transcribed_cluster

InterPro

Analysis Name: InterPro Annotations of melon
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002160	Proteinase inhibitor I3, Kunitz legume	PRINTS	PR00291	KUNITZINHBTR	coord: 151..170 score: 6.8E-19coord: 174..203 score: 6.8E-19coord: 31..60 score: 6.8E-19coord: 69..89 score: 6.8
IPR002160	Proteinase inhibitor I3, Kunitz legume	PFAM	PF00197	Kunitz_legume	coord: 32..204 score: 2.7
IPR002160	Proteinase inhibitor I3, Kunitz legume	SMART	SM00452	kul_2	coord: 31..205 score: 1.2
IPR002160	Proteinase inhibitor I3, Kunitz legume	PROSITE	PS00283	SOYBEAN_KUNITZ	coord: 32..48 scor
IPR011065	Kunitz inhibitor ST1-like	unknown	SSF50386	STI-like	coord: 28..205 score: 1.14
None	No IPR available	GENE3D	G3DSA:2.80.10.50		coord: 28..205 score: 1.2
None	No IPR available	PANTHER	PTHR33107	FAMILY NOT NAMED	coord: 3..205 score: 2.5

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
MELO3C002175	Silver-seed gourd	carmeB0393
MELO3C002175	Silver-seed gourd	carmeB0499
MELO3C002175	Cucumber (Chinese Long) v3	cucmeB024
MELO3C002175	Watermelon (97103) v2	mewmbB133
MELO3C002175	Wax gourd	mewgoB173
MELO3C002175	Cucumber (Gy14) v1	cgymeB185
MELO3C002175	Cucurbita maxima (Rimu)	cmameB193
MELO3C002175	Cucurbita maxima (Rimu)	cmameB365
MELO3C002175	Cucurbita moschata (Rifu)	cmomeB180
MELO3C002175	Cucurbita moschata (Rifu)	cmomeB356
MELO3C002175	Wild cucumber (PI 183967)	cpimeB024
MELO3C002175	Cucumber (Chinese Long) v2	cumeB027
MELO3C002175	Watermelon (Charleston Gray)	mewcgB130
MELO3C002175	Watermelon (97103) v1	mewmB166
MELO3C002175	Cucurbita pepo (Zucchini)	cpemeB026
MELO3C002175	Cucurbita pepo (Zucchini)	cpemeB478
MELO3C002175	Bottle gourd (USVL1VR-Ls)	lsimeB179
MELO3C002175	Cucumber (Gy14) v2	cgybmeB022