LsiUNG001000 (gene) Bottle gourd (USVL1VR-Ls)

NameLsiUNG001000
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionE3 ubiquitin-protein ligase XBAT32
Locationchr00 : 2726806 .. 2731961 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTCGCATTTCCTTGTGCTCTCGTCACTAAGAAAAATTGGCGCCCAAATTAGAGTGCGAATGTTGAATTTACCATTCAACAACAGTTCTTGGAGGTACGAAGGAAAGGGAATGGAGGGAAGCAATGATTTGGAAGTTCTTGGAAGAATTAAACGAGTTAACCCGGGAACTCGGTGGACTAAAAACGAAAAGAGGAAAGCCCCTTGGAGGGGACAACTACGCCTGGAGAGGAAAACAACAACTCAACTACCGAGTTGACCCAACATAATATCTAATCATTGAGACACGGTTTGACGCGAGGCTGTAGGAAAAGGTCATCCTCTCCATTTAAACAATTTTCCACTTGTGATTTCTTGTTCGCATTCCATTCATACTTTTATGGCAATTATGATTTAATGTTGCAACATTCTTCAATAATCATCATAACTTTGCTTCCTTCTTAGCTATCTTCTTTGAGAAGTTGCAGAGTTTTCAAGGTGAAACTGCTTTCTCTGTTACCTTATCACAAACCCTTCTTGTAGCCATATTTCAAAATTGCTTCTCGCATTGACATTCTTCCTTGGAGTAGATTTAGGCTTCGTCTTTGGAGATTCTTTTGTTATTGATAAAAATCAAGAAGCAGGCCCTGTTATTAGATATTTAGAAATGGGGTGGCTTTGACGGGGAAGACAAACTTCTATTTCATCAGCTATTGAAGTTCACGAAATTTGGTTAATTTCTTATTCATTACCACAAAACAGGTTATGCTGGTTTGAACTTTGAAGCTAGATATTCCCTTTTCTTCTTACTAGCCTATCATGATTCATTATCTTAGATTGGTGAACCAACTGTTTTGGATGTCTGAAAGATTTCATTCTTAGTTGTTATTTGTTTGGTTAAAACGAAAAGAAAAAAAAAAAAAAAGAAAAGAAAAATTGGTGCATGGATAGGTCTCTTTTCCATCCATACTTTGTGTAGCCTTTTTTAAATACATTCGAAAGAGATCATCATCTCTGGAGTTTCTTCGAAGCTGTTAGTTTCTTCAAATTGATTTATCAGTTTCGAGTTAAAAAATTTGAATGCGATGAAACTTTTAAGTGCTGTGGTAAACTCCTTTGGATGTTCTGCATCTGGGGAACGTTTAGTTTCAGCTGCAAGAGATGGAGATTTTCAAGAAGCACAGGCTTTGTTGGAATATAATCCACGGTTGGCACGTTACTCTACTTTCGGTGTGCGCAATTCCCCACTCCACTACTCTGCAGCTCATGGCCATCACGAGGTATTTCACTCTTTAAATGCACACTGATAATGTTTGTGAAGCCATTCTCACTTCGTTTAAGATCAGAGAATCTTATAGCCTGTTGTCATTGTGCTTGTTCTTACATGTTTGCAGATAGTCTATCTTTTGCTTCAGTCTGGAGTTGACATTAATTTAAGAAACTATAGGGGTCAGGTATTGCCACTTTCTTCAAATTATTTGCCTTTTCATTTCAGGGATCTACATCCATAAGTGTTTTTTCTTCTCTTTTCACCATTTCATTTTCTCCATATTGTAATTATCTGTGCTATTGGACGTTAAGTTGTATCTCCTGCATTTCAAGATTAAATTCATTATGTATTTTGAGTTCATCATTGTTTGCTTTACACTTCTGTACACAGACTGCTTTGATGCAAGCTTGTCAACATGGTCGCTGGGAGGTTGTTTTGATTTTAGTACTTTTTGGAGCCAACGTGAGTAGCTTATCACATTCATTTTAGCTATCACGTTCATAGTGTGAGCAACTTATTAGTCATTCATATATTTGTTTCACATTTTAGGTCCATAAAGCAGATTATTTGAATGGAGGGACTGCACTTCATCTTGCTGCTACAAATGGTCATTCCCGGTGCATCAGGCTTCTTCTTGCTGATTATATTCCAAGCATACCTAATTTTAGGGAAATAATGAGTCAATTAACAGACAATGAAGAATCAATCTCAGAGTTTGATCATAGGTACATATTGAATTCCTCCTCATGAAAATTTTCCAGGTTGAAATGCTTATGATGGTTGATTAACATCATAATTTTGTGGTTTAGGGCACTCTCTCAGATTATTAACCAAACGGCAGATGGAGGCATTACCGCTCTTCACATGGCAGCCCTTAATGGGCAAGTTGAAAGCGTGCAGTTACTTCTGGAATTTGGGGCATCAGTTTCTGAGGTGACTCTAGGCGATGGAACCGTAATTGATCTAATAGGTTTCTCCCTTTGATTTTTTTTGTCTTCTCTTTCACTAAATCAACTTGAGGCCTTCTTTTTCAAGTTAGCCTTTATTTTCCTTCCTAATCCACCACTCAACTTGTTTTCTTTTTTCTTTTAAACTTCTTTTGAGAGTAACAAAACATGAATCTAAAATCTTGACATTATGTTTTGGATTTTCTTCTATTTTCATATAAAGGCTAGAGCTAGCATGCATTGTCATGGGATGTTGAAATGAAGTGGCAGTTAAATGCAATGTTATTTCTTTTGCCTCAGTAAAATATGCTACCTTATCCTTCTATTCTGTGGGCAAGTGAAAAGTACTGATAAAAGGGGTTATTGAAAAGTTGATTTCTTATGATCTCTTGCGATGATATAAATGGCTTGTCTGTTGCAACTTCGGTAGGTGCTGGGAGTACAGCTCTTCATTATGCTGCATGCGGTGGTAATGCAAAATGTTGTCAAGTATGTGTTTCATTTAAAGGAAAAGAATCAGAAATGGGATTAAATGAATGTATTGTGTTTTCTTCTTTGAGAGGCTATTACTTATTGTTTTCTATTTCGTGTAGCTTTTGATTGCGAGGGGGGCCAGTCTGACTGCTGAAAATGAAAATGGGTACGTACTATCCACTTGGCTTATTCTTTGTATGTGAACTTGAATCTTTTTTTTTTTAATCTTTGGACCTGTGCCTAGCAAGTCACCTTCTGAACATAAGAAATTGCTTTTAAGAAATGAGTTTACGTTCTTTTAATTACTTGGGCACTCATTAGGGAACTATTTAAAAAACTGAAAGCGGTAGTGGCTGTGGAGAAATTGATAGGAATGCTGTTAGGATAGAAGTTAAGGATATTATTATGCTAGGAAGGGTATTAGTTTATTTGGTTTAGGCTTGAATGATCTCGAGAGAGGAAGAGTCTATGAACCTCAAATTACTTTAAGAGTTATTGTAGTTTTTCTGTGTATTATTATAATGCCAACTCCAAGTATTTGGGTTCTATCAGAAATTTTGGAGGATTCCCATTAAAATTTTAAAGTGAAACAGTGTCAACAGCCTTGTCAATAAGTATATGCTGGCTTATGTGATTGTCTGGCTAGCTTGAGATGGAAGAATTGGGGTTCAAATTGAAAGCAGTAGCGGCTATGGAGAAATTTTGGAAGACTCCCCTTTTTAGTTCCTTGAAAACACATTACATGAATGCCTCAATTACATAAGAAATTTGTGTGGTTGAACGGTTAATTCATAGGGTTCCCTATTTCCAAGGATATTAATGGGTCACTCATTTCACATCAATAACATTCTGAGAATACTATATAAGTTTTCGATGTTCGTTTAACTACATTAAGCACGGGTATAATCCTTTGATTACACTGATCATTTTGTCATGGAAATATTGTTCCCTACTTTGTATGGAATTGTAGCATTCACTTGATTTAGCATGGTTATCGTATAGCAATCCCTTTATCCATTCTTTTATCAACTTGCATCTACTTCACTTGATTTGTCTTTTTTTCCCACACAGTTGGACACCATTGATGGTCGCTCGCTCATGGAATAAAAATTGGCTTGAGGATATTCTCACCGCAGAGCCAGTAGACCGGCCAAAAGTTATTCCCTCTCGTTACATATCTCTGCCCCTTATGAGTATTGTTCAAATTGCTAGGTAACTGGAATTCGTTATTTCCTTCTATTGCTTATCCTTCCAAAGAAATTTATATACATGGAATCGACTTGCCCTTCATTTATTCATTCTGGAGCCACCAACTTTGAGGACAATATTGTGCGCTTCTGGTTACTACATCATAGAATTTGATCCACATGCTCCTCACTCTGGTCCTCAAGATTAAGTTCATATTGGGTTGACATAGAACAAGATATTGAAGACAAGCTATTAGAACCAGTCCTAAAATATAAGTTGTTTAGAAAATCAAAGAACAAACTAGAAAATTGTTCTCTCTCTACATATAGCTTGCAGATATTGCGTCCAATGAATTTAGAACGTTGTCATGATGAATGAAATTGATTTCGGTATCCCTTTTGGATCCATTTGCAGAGAGTATGGATGGACTATTACGTCTGCCCCCGGATGCCAGGATCCATGCGTTGTCTGTTTAGAGAGGAAGTGCACGGTAGCTGCTGAAGGTATATTATTAACTTAACAATATATATACTTGACTAGAGAATTATGAAATTCTTATTTCCTTAACTTGCATAACCCGATTTATTTATGTTCCATTAAAGTTTTGACAAGTTTGCTGAGTATGAGCAAAAGTTTGAACTTCAATGTTTAAAACATGGGCTATTTTCATATTAGTTAATGAAATTTATTCATAATGGATTTAAAAGTGCAGTGATGGTAAAGATTTGATTGAAAAGAGCTGATATAGATGAAAGTTGAAACAAGCTTTTGAAATAACAAGCTTTTGATATAGATGAAAGTAGTAAACTAAATTCTATACTCAGCCCATCTATTACGTCTATTGGTATGTGGGGTTGTTGAAGTATGATAAGAAGTCCAATCCCCTGCAGGCTGTGGTCATGAATTCTGTACAAGATGTGCCTTATACCTCTGTTCTACAAACTGCGGGACAACCATTTCTCGTGGCCCTCCAGGTTCAGTTTCTTGTCCACTCTGTCGAAATGGCATAGTGTCATTCGTTAAGCTTCCTGGAAAAGCACTTGTTAAAGAGATTGCAAGAACAAGTTTGTCACTTTCAATGTGCCAATGTTCTCACGAGACACCATCAAAACCTACCCCCTTAACCACCCAACTTCAATCGCTCGAGTTGTGTCGTGTCCACGATACTCCTCTGGCAGTTTCCCTTTGCTGTCAGAGACTTCCATCAATCAATCTCTGTCGGAAAACACCGGACATATGCCACTCTTTAGTTCCACGTTGTGCCAACCGGAACTTGCAGAACCACTTGGCCAGATGTTGA

mRNA sequence

ATTCGCATTTCCTTGTGCTCTCGTCACTAAGAAAAATTGGCGCCCAAATTAGAGTGCGAATGTTGAATTTACCATTCAACAACAGTTCTTGGAGGTACGAAGGAAAGGGAATGGAGGGAAGCAATGATTTGGAAGTTCTTGGAAGAATTAAACGAGTTAACCCGGGAACTCGGTGGACTAAAAACGAAAAGAGGAAAGCCCCTTGGAGGGGACAACTACGCCTGGAGAGGAAAACAACAACTCAACTACCGAGTTGACCCAACATAATATCTAATCATTGAGACACGGTTTGACGCGAGGCTGTAGGAAAAGGTCATCCTCTCCATTTAAACAATTTTCCACTTGTGATTTCTTGTTCGCATTCCATTCATACTTTTATGGCAATTATGATTTAATGTTGCAACATTCTTCAATAATCATCATAACTTTGCTTCCTTCTTAGCTATCTTCTTTGAGAAGTTGCAGAGTTTTCAAGGTGAAACTGCTTTCTCTGTTACCTTATCACAAACCCTTCTTGTAGCCATATTTCAAAATTGCTTCTCGCATTGACATTCTTCCTTGGAGTAGATTTAGGCTTCGTCTTTGGAGATTCTTTTGTTATTGATAAAAATCAAGAAGCAGGCCCTGTTATTAGATATTTAGAAATGGGGTGGCTTTGACGGGGAAGACAAACTTCTATTTCATCAGCTATTGAAGTTCACGAAATTTGGTTAATTTCTTATTCATTACCACAAAACAGGTTATGCTGGTTTGAACTTTGAAGCTAGATATTCCCTTTTCTTCTTACTAGCCTATCATGATTCATTATCTTAGATTGGTGAACCAACTGTTTTGGATGTCTGAAAGATTTCATTCTTAGTTGTTATTTGTTTGGTTAAAACGAAAAGAAAAAAAAAAAAAAAGAAAAGAAAAATTGGTGCATGGATAGGTCTCTTTTCCATCCATACTTTGTGTAGCCTTTTTTAAATACATTCGAAAGAGATCATCATCTCTGGAGTTTCTTCGAAGCTGTTAGTTTCTTCAAATTGATTTATCAGTTTCGAGTTAAAAAATTTGAATGCGATGAAACTTTTAAGTGCTGTGGTAAACTCCTTTGGATGTTCTGCATCTGGGGAACGTTTAGTTTCAGCTGCAAGAGATGGAGATTTTCAAGAAGCACAGGCTTTGTTGGAATATAATCCACGGTTGGCACGTTACTCTACTTTCGGTGTGCGCAATTCCCCACTCCACTACTCTGCAGCTCATGGCCATCACGAGATAGTCTATCTTTTGCTTCAGTCTGGAGTTGACATTAATTTAAGAAACTATAGGGGTCAGACTGCTTTGATGCAAGCTTGTCAACATGGTCGCTGGGAGGTTGTTTTGATTTTAGTACTTTTTGGAGCCAACGTCCATAAAGCAGATTATTTGAATGGAGGGACTGCACTTCATCTTGCTGCTACAAATGGTCATTCCCGGTGCATCAGGCTTCTTCTTGCTGATTATATTCCAAGCATACCTAATTTTAGGGAAATAATGAGTCAATTAACAGACAATGAAGAATCAATCTCAGAGTTTGATCATAGGGCACTCTCTCAGATTATTAACCAAACGGCAGATGGAGGCATTACCGCTCTTCACATGGCAGCCCTTAATGGGCAAGTTGAAAGCGTGCAGTTACTTCTGGAATTTGGGGCATCAGTTTCTGAGGTGACTCTAGGCGATGGAACCGTAATTGATCTAATAGGTGCTGGGAGTACAGCTCTTCATTATGCTGCATGCGGTGGTAATGCAAAATGTTGTCAACTTTTGATTGCGAGGGGGGCCAGTCTGACTGCTGAAAATGAAAATGGTTGGACACCATTGATGGTCGCTCGCTCATGGAATAAAAATTGGCTTGAGGATATTCTCACCGCAGAGCCAGTAGACCGGCCAAAAGTTATTCCCTCTCGTTACATATCTCTGCCCCTTATGAGTATTGTTCAAATTGCTAGAGAGTATGGATGGACTATTACGTCTGCCCCCGGATGCCAGGATCCATGCGTTGTCTGTTTAGAGAGGAAGTGCACGGTAGCTGCTGAAGGCTGTGGTCATGAATTCTGTACAAGATGTGCCTTATACCTCTGTTCTACAAACTGCGGGACAACCATTTCTCGTGGCCCTCCAGGTTCAGTTTCTTGTCCACTCTGTCGAAATGGCATAGTGTCATTCGTTAAGCTTCCTGGAAAAGCACTTGTTAAAGAGATTGCAAGAACAAGTTTGTCACTTTCAATGTGCCAATGTTCTCACGAGACACCATCAAAACCTACCCCCTTAACCACCCAACTTCAATCGCTCGAGTTGTGTCGTGTCCACGATACTCCTCTGGCAGTTTCCCTTTGCTGTCAGAGACTTCCATCAATCAATCTCTGTCGGAAAACACCGGACATATGCCACTCTTTAGTTCCACGTTGTGCCAACCGGAACTTGCAGAACCACTTGGCCAGATGTTGA

Coding sequence (CDS)

ATGAAACTTTTAAGTGCTGTGGTAAACTCCTTTGGATGTTCTGCATCTGGGGAACGTTTAGTTTCAGCTGCAAGAGATGGAGATTTTCAAGAAGCACAGGCTTTGTTGGAATATAATCCACGGTTGGCACGTTACTCTACTTTCGGTGTGCGCAATTCCCCACTCCACTACTCTGCAGCTCATGGCCATCACGAGATAGTCTATCTTTTGCTTCAGTCTGGAGTTGACATTAATTTAAGAAACTATAGGGGTCAGACTGCTTTGATGCAAGCTTGTCAACATGGTCGCTGGGAGGTTGTTTTGATTTTAGTACTTTTTGGAGCCAACGTCCATAAAGCAGATTATTTGAATGGAGGGACTGCACTTCATCTTGCTGCTACAAATGGTCATTCCCGGTGCATCAGGCTTCTTCTTGCTGATTATATTCCAAGCATACCTAATTTTAGGGAAATAATGAGTCAATTAACAGACAATGAAGAATCAATCTCAGAGTTTGATCATAGGGCACTCTCTCAGATTATTAACCAAACGGCAGATGGAGGCATTACCGCTCTTCACATGGCAGCCCTTAATGGGCAAGTTGAAAGCGTGCAGTTACTTCTGGAATTTGGGGCATCAGTTTCTGAGGTGACTCTAGGCGATGGAACCGTAATTGATCTAATAGGTGCTGGGAGTACAGCTCTTCATTATGCTGCATGCGGTGGTAATGCAAAATGTTGTCAACTTTTGATTGCGAGGGGGGCCAGTCTGACTGCTGAAAATGAAAATGGTTGGACACCATTGATGGTCGCTCGCTCATGGAATAAAAATTGGCTTGAGGATATTCTCACCGCAGAGCCAGTAGACCGGCCAAAAGTTATTCCCTCTCGTTACATATCTCTGCCCCTTATGAGTATTGTTCAAATTGCTAGAGAGTATGGATGGACTATTACGTCTGCCCCCGGATGCCAGGATCCATGCGTTGTCTGTTTAGAGAGGAAGTGCACGGTAGCTGCTGAAGGCTGTGGTCATGAATTCTGTACAAGATGTGCCTTATACCTCTGTTCTACAAACTGCGGGACAACCATTTCTCGTGGCCCTCCAGGTTCAGTTTCTTGTCCACTCTGTCGAAATGGCATAGTGTCATTCGTTAAGCTTCCTGGAAAAGCACTTGTTAAAGAGATTGCAAGAACAAGTTTGTCACTTTCAATGTGCCAATGTTCTCACGAGACACCATCAAAACCTACCCCCTTAACCACCCAACTTCAATCGCTCGAGTTGTGTCGTGTCCACGATACTCCTCTGGCAGTTTCCCTTTGCTGTCAGAGACTTCCATCAATCAATCTCTGTCGGAAAACACCGGACATATGCCACTCTTTAGTTCCACGTTGTGCCAACCGGAACTTGCAGAACCACTTGGCCAGATGTTGA

Protein sequence

MKLLSAVVNSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAAHGHHEIVYLLLQSGVDINLRNYRGQTALMQACQHGRWEVVLILVLFGANVHKADYLNGGTALHLAATNGHSRCIRLLLADYIPSIPNFREIMSQLTDNEESISEFDHRALSQIINQTADGGITALHMAALNGQVESVQLLLEFGASVSEVTLGDGTVIDLIGAGSTALHYAACGGNAKCCQLLIARGASLTAENENGWTPLMVARSWNKNWLEDILTAEPVDRPKVIPSRYISLPLMSIVQIAREYGWTITSAPGCQDPCVVCLERKCTVAAEGCGHEFCTRCALYLCSTNCGTTISRGPPGSVSCPLCRNGIVSFVKLPGKALVKEIARTSLSLSMCQCSHETPSKPTPLTTQLQSLELCRVHDTPLAVSLCCQRLPSINLCRKTPDICHSLVPRCANRNLQNHLARC
BLAST of LsiUNG001000 vs. Swiss-Prot
Match: XB32_ORYSJ (Probable E3 ubiquitin-protein ligase XBOS32 OS=Oryza sativa subsp. japonica GN=XBOS32 PE=2 SV=2)

HSP 1 Score: 563.1 bits (1450), Expect = 2.8e-159
Identity = 289/456 (63.38%), Postives = 343/456 (75.22%), Query Frame = 1

Query: 1   MKLLSAVVNSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60
           M  LS V NSFGCSASGERLVSAARDGD QEA+ALLEYNPRLARYSTFG RNSPLHY+AA
Sbjct: 1   MGFLSLVGNSFGCSASGERLVSAARDGDLQEARALLEYNPRLARYSTFGGRNSPLHYAAA 60

Query: 61  HGHHEIVYLLLQSGVDINLRNYRGQTALMQACQHGRWEVVLILVLFGANVHKADYLNGGT 120
            GHHEIV LLL+SGV+INLRNYRGQTALMQACQ+G WEVV  L+LF ANVH+ DYLNGG+
Sbjct: 61  QGHHEIVSLLLESGVEINLRNYRGQTALMQACQYGHWEVVQTLMLFNANVHRTDYLNGGS 120

Query: 121 ALHLAATNGHSRCIRLLLADYIPSIPNFREIMSQLTDNEESISEFDHRALSQIINQTADG 180
           ALH AA +GH+RC+RL+LADY+PS+PNF   M      E   ++ D   L +++NQ ADG
Sbjct: 121 ALHFAALHGHARCLRLVLADYVPSMPNFWNSMKDSLSEEGPSADLDEDGLFKMVNQKADG 180

Query: 181 GITALHMAALNGQVESVQLLLEFGASVSEVTLGDGTVIDLIGAGSTALHYAACGGNAKCC 240
           G+T LHMAALNG VE VQLLL+ GASV E T+ DGT IDLIGAGST LHYAACGGNA CC
Sbjct: 181 GLTPLHMAALNGHVECVQLLLDLGASVIEATIEDGTTIDLIGAGSTPLHYAACGGNAVCC 240

Query: 241 QLLIARGASLTAENENGWTPLMVARSWNKNWLEDILTAEPVDRPKVIPSRYISLPLMSIV 300
           QLLIARGASL+A+N +GWTPLMVARSW++N LE+IL+ EP  R + +PS Y+ LPLMSI+
Sbjct: 241 QLLIARGASLSAQNASGWTPLMVARSWHRNSLEEILSKEPESRIRTVPSPYLCLPLMSIM 300

Query: 301 QIAREYGWT-ITSAPGCQDPCVVCLERKCTVAAEGCGHEFCTRCALYLCSTNCGTTISRG 360
            IARE+GW  +  +P C DPC VCLE  C+VAAEGC HEFCTRCALYLCST+  T++S  
Sbjct: 301 SIAREFGWRYLNQSPVCIDPCAVCLEGSCSVAAEGCKHEFCTRCALYLCSTSY-TSVS-- 360

Query: 361 PPGSVSCPLCRNGIVSFVKLPGKALVKEIARTSLSLSMCQCSHETPSKPTP-LTTQLQSL 420
           P G++ CPLCR+ I++F  LPG + ++E+ R SLSLS C       S  TP + + L   
Sbjct: 361 PAGAIPCPLCRHPIIAFTALPGTSPIRELPRNSLSLSFCTTCPAVNSDSTPSIASHLYRT 420

Query: 421 ELCRVHDTPLAV----SLCCQRLPSINLCRKTPDIC 451
           E       P+      SL CQRLP++ L    P  C
Sbjct: 421 EFQCARMPPMGSSSFRSLSCQRLPAMKL---NPSFC 450

BLAST of LsiUNG001000 vs. Swiss-Prot
Match: XB32_ARATH (E3 ubiquitin-protein ligase XBAT32 OS=Arabidopsis thaliana GN=XBAT32 PE=1 SV=1)

HSP 1 Score: 558.1 bits (1437), Expect = 8.9e-158
Identity = 279/411 (67.88%), Postives = 327/411 (79.56%), Query Frame = 1

Query: 1   MKLLSAVVNSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60
           M+ LS V NSFGCSASGERLVSAARDGD QEA+ALL+YNPRLARYSTFGVRNSPLHYSAA
Sbjct: 1   MRFLSLVGNSFGCSASGERLVSAARDGDLQEAKALLDYNPRLARYSTFGVRNSPLHYSAA 60

Query: 61  HGHHEIVYLLLQSGVDINLRNYRGQTALMQACQHGRWEVVLILVLFGANVHKADYLNGGT 120
            GHHEIV LL++SGVDINLRNYRGQTALMQACQHG WEVVLIL+LFGAN+H++DYLNGGT
Sbjct: 61  QGHHEIVSLLVESGVDINLRNYRGQTALMQACQHGHWEVVLILILFGANIHRSDYLNGGT 120

Query: 121 ALHLAATNGHSRCIRLLLADYIPSIPNFREIMSQLTDNEESISEFDHRALSQIINQTADG 180
           ALHLAA NGH RCIR+LL++YIPS+PN     S L + + S++ FD   L ++IN+ ADG
Sbjct: 121 ALHLAALNGHPRCIRILLSEYIPSVPN---CWSLLKNKKTSVAGFDSSVLHEVINRAADG 180

Query: 181 GITALHMAALNGQVESVQLLLEFGASVSEVTLGDGTVIDLIGAGSTALHYAACGGNAKCC 240
           GIT LH+AALNG +E+VQLLL+ GASV++VT+ DGT IDLIGAGSTALHYA+CGGN +CC
Sbjct: 181 GITPLHVAALNGHIETVQLLLDLGASVTQVTVEDGTTIDLIGAGSTALHYASCGGNTQCC 240

Query: 241 QLLIARGASLTAENENGWTPLMVARSWNKNWLEDIL---TAEPVDRPKVIPSRYISLPLM 300
           QLLI++GA L A N NGWTP+MVARSW++NWLE+IL   T +P      +PS ++ LPLM
Sbjct: 241 QLLISKGACLAAVNSNGWTPMMVARSWHRNWLEEILNPTTEQPQLHLPNVPSPFLCLPLM 300

Query: 301 SIVQIAREYGWTITSA-PGCQDPCVVCLERKCTVAAEGCGHEFCTRCALYLCSTN-CGTT 360
           SIV IA+E GW        C+DPC VCLERKCTVAA+GC HEFCT CALYL +T+   + 
Sbjct: 301 SIVNIAQECGWRENDCLTPCRDPCAVCLERKCTVAADGCAHEFCTNCALYLSTTSITSSK 360

Query: 361 ISRGPPGSVSCPLCRNGIVSFVKLP---GKALVKEIARTSLSLSMCQCSHE 404
            S   PGSV CPLCRNGIVSF KLP           +RTS+SLS C CS +
Sbjct: 361 TSNVTPGSVPCPLCRNGIVSFTKLPHTTATTRTSTSSRTSISLSFCTCSSD 408

BLAST of LsiUNG001000 vs. Swiss-Prot
Match: XB35_ORYSJ (Probable E3 ubiquitin-protein ligase XBOS35 OS=Oryza sativa subsp. japonica GN=XBOS35 PE=2 SV=1)

HSP 1 Score: 524.6 bits (1350), Expect = 1.1e-147
Identity = 259/408 (63.48%), Postives = 315/408 (77.21%), Query Frame = 1

Query: 1   MKLLSAVVNSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60
           M LL  V +SFGCSA+GERLVSAARDGD QEA ALLE NPRLARYSTFG+RNSPLHYSAA
Sbjct: 1   MGLLGMVGDSFGCSATGERLVSAARDGDIQEAMALLELNPRLARYSTFGIRNSPLHYSAA 60

Query: 61  HGHHEIVYLLLQSGVDINLRNYRGQTALMQACQHGRWEVVLILVLFGANVHKADYLNGGT 120
            GHHEIV LL++SGVDINLRN RGQTALMQAC +G W+VV ILVLF AN+HK D  +G T
Sbjct: 61  KGHHEIVSLLIESGVDINLRNCRGQTALMQACLYGHWKVVQILVLFKANIHKKDCFSGAT 120

Query: 121 ALHLAATNGHSRCIRLLLADYIPSIPNFREIMSQLTDNEESISEFDHRALSQIINQTADG 180
           A+H AA  GH+RC+RLL+ADY+PS+P F  +M     +E +   FD  AL ++IN  +DG
Sbjct: 121 AIHFAALKGHTRCLRLLVADYVPSLPEFWSVMHAKCTDETNKEAFDAVALRRLINNKSDG 180

Query: 181 GITALHMAALNGQVESVQLLLEFGASVSEVTLGDGTVIDLIGAGSTALHYAACGGNAKCC 240
           G+T LH+AAL+G  E VQLLL+ GASVSEVT+ DG+ IDLIG+GST LHYAACGG+A CC
Sbjct: 181 GVTPLHLAALHGHAECVQLLLDLGASVSEVTINDGSTIDLIGSGSTPLHYAACGGSAVCC 240

Query: 241 QLLIARGASLTAENENGWTPLMVARSWNKNWLEDILTAEPVDRPKVIPSRYISLPLMSIV 300
           QLL+A GA++ A+N NG TPLMVARSW+K+ +E ILT       +++PS Y+SLPLMSIV
Sbjct: 241 QLLVAAGANMRAQNTNGLTPLMVARSWHKSSVEGILTKRSEVPVRILPSSYLSLPLMSIV 300

Query: 301 QIAREYGWTITSAPG-CQDPCVVCLERKCTVAAEGCGHEFCTRCALYLCSTNCGTTISRG 360
           +IARE GW  TS    C DPC +CL+ +CTV+AEGCGHEFCT+CALYLC+T   +T  RG
Sbjct: 301 KIARECGWRKTSVSSVCHDPCAICLDTECTVSAEGCGHEFCTKCALYLCATASSSTSIRG 360

Query: 361 PPGSVSCPLCRNGIVSFVKLPGKALVKEIARTSLSLSMCQCSHETPSK 408
            PGS+ CPLCR+ IVSFV+L     +KE+  T+ SL++C     T SK
Sbjct: 361 VPGSIPCPLCRHTIVSFVRLTSTTPIKELPWTNKSLALCAAGASTGSK 408

BLAST of LsiUNG001000 vs. Swiss-Prot
Match: XB33_ARATH (E3 ubiquitin-protein ligase XBAT33 OS=Arabidopsis thaliana GN=XBAT33 PE=2 SV=1)

HSP 1 Score: 443.7 bits (1140), Expect = 2.4e-123
Identity = 245/470 (52.13%), Postives = 315/470 (67.02%), Query Frame = 1

Query: 9   NSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAAHGHHEIVY 68
           NSFGCSASGERLVSAARDGDF EA+ LL+ NP LA+YSTFG  NSPLH++AA GH+EIV 
Sbjct: 3   NSFGCSASGERLVSAARDGDFVEAKMLLDCNPCLAKYSTFGGLNSPLHFAAAKGHNEIVG 62

Query: 69  LLLQSGVDINLRNYRGQTALMQACQHGRWEVVLILVLFGANVHKADYLNGGTALHLAATN 128
           LLL++G D+N RNY GQTALMQAC++G WEVV  L+LF  NV +ADYL G TALH AA N
Sbjct: 63  LLLENGADVNSRNYCGQTALMQACRYGHWEVVQTLLLFRCNVTRADYLAGRTALHFAAVN 122

Query: 129 GHSRCIRLLLADYIPSIPNFREIMSQL--TDNEESISEFDHRALSQIINQTADGGITALH 188
           GH+RCIRL+LAD++PS     + ++ L  T    + ++ +  ALS+ +N+ ADGGITALH
Sbjct: 123 GHARCIRLVLADFLPS-----DKLNSLPETGVVTAKNKSEQSALSKFVNKAADGGITALH 182

Query: 189 MAALNGQVESVQLLLEFGASVSEVTLGDGTVIDLIGAGSTALHYAACGGNAKCCQLLIAR 248
           MAALNG  + VQLLL+  A+VS VT   GT +D+IGAGST LHYAACGGN KCCQ+L+AR
Sbjct: 183 MAALNGLFDCVQLLLDLEANVSAVTFHYGTSMDMIGAGSTPLHYAACGGNLKCCQILLAR 242

Query: 249 GASLTAENENGWTPLMVARSWNKNWLEDILTA-EPVDRPKVIPSRYISLPLMSIVQIARE 308
           GA     N NGW P+ +AR W+++WLE +L+    V  P    S Y+SLPL+SI+ IARE
Sbjct: 243 GARKMTLNCNGWLPIDIARMWSRHWLEPLLSPNSDVVIPAFPHSNYLSLPLLSILNIARE 302

Query: 309 YGWTITSAPGCQDPCVVCLERKCTVAAEGCGHEFCTRCALYLCSTNCGTTISRGPPGSVS 368
           +G    +     D C VCLER CTVAAEGC H+ C RCALYLCS++   +++ GPPGS+ 
Sbjct: 303 FGLQSATIGDEVDICAVCLERTCTVAAEGCEHQLCVRCALYLCSSSNVPSVTVGPPGSIP 362

Query: 369 CPLCRNGIVSFVKLPGKALVKEIARTSLSLSMC-QCSHET-----PSKPT-PLTTQLQSL 428
           CPLCR+GI +F +LP  +L +E+ +  +SL  C  C   T      S PT P T Q  S 
Sbjct: 363 CPLCRHGITAFKRLP-SSLTREM-KLPMSLGFCAPCMLHTGDTTDQSSPTCPPTEQRSSK 422

Query: 429 ELCRVHDTPLAVSLCCQRLPSIN--LCRKTPDICHSLVPRCANRNLQNHL 467
                  + +   + C   PS+N  +C      C +       R+ + H+
Sbjct: 423 TRAASVSSDIFCPVTCSPFPSVNIPMCTCNEGTCPNFETHGTERHSEEHV 465

BLAST of LsiUNG001000 vs. Swiss-Prot
Match: XB33_ORYSJ (Probable E3 ubiquitin-protein ligase XBOS33 OS=Oryza sativa subsp. japonica GN=XBOS33 PE=2 SV=1)

HSP 1 Score: 426.0 bits (1094), Expect = 5.3e-118
Identity = 242/459 (52.72%), Postives = 300/459 (65.36%), Query Frame = 1

Query: 9   NSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAAHGHHEIVY 68
           NS GCSASGERLVSAARDGD  EA+ LLE +P LARYSTFG  NSPLH++AA GH +IV 
Sbjct: 3   NSLGCSASGERLVSAARDGDAVEARMLLELSPALARYSTFGGLNSPLHFAAAKGHLDIVT 62

Query: 69  LLLQSGVDINLRNYRGQTALMQACQHGRWEVVLILVLFGANVHKADYLNGGTALHLAATN 128
           LLL+ G D+N+RNY GQTALM AC+HG WEVV +L+LF  NV +ADYL+G TALH AA +
Sbjct: 63  LLLEKGADVNVRNYCGQTALMHACRHGHWEVVQMLLLFRCNVTRADYLSGRTALHFAAHD 122

Query: 129 GHSRCIRLLLADYIPSIPNFREIMSQLTDNEESIS------------EFDHRALSQIINQ 188
           G  RC+RLLLAD++PS P   +  S   D  E  +            +F+  A  + IN+
Sbjct: 123 GLVRCVRLLLADFVPSAP-LEDGASSTVDGGECQTNSGSSPCSSLGLKFNESARLRYINK 182

Query: 189 TADGGITALHMAALNGQVESVQLLLEFGASVSEVTLGDGTVIDLIGAGSTALHYAACGGN 248
            ADGG+TALHMAALNG  + +QLL++ GA+VS VT   GT  +LIGAGST LHYAA GGN
Sbjct: 183 PADGGVTALHMAALNGHFDCMQLLIDLGANVSAVTFPYGTTANLIGAGSTPLHYAAGGGN 242

Query: 249 AKCCQLLIARGASLTAENENGWTPLMVARSWNKNWLEDILTAEPVDR-PKVIPSRYISLP 308
           A+CCQLL+++GAS    N NGW P+ VAR + + +LE +L+       P   PS Y++LP
Sbjct: 243 AECCQLLLSKGASKLTLNCNGWLPIDVARMFGRRFLEPLLSPNSNSSIPAYQPSNYLALP 302

Query: 309 LMSIVQIAREYGWTITSAP-GCQDPCVVCLERKCTVAAEGCGHEFCTRCALYLCSTNCGT 368
            MSI+ IARE+G   T A     D C VCLER C+VAAEGC HEFC +CALYLCST+   
Sbjct: 303 FMSILNIAREFGLLHTVASVDDSDLCAVCLERSCSVAAEGCCHEFCIKCALYLCSTSNTR 362

Query: 369 TISRGPPGSVSCPLCRNGIVSFVKLPGKALVKEIARTSLSLSMCQ-CSHETPSKPTPLTT 428
               GPPGS+ CPLCRNGI+SF KLP  +   E  ++S +L+ C  C   T S  +P T 
Sbjct: 363 VEFTGPPGSIPCPLCRNGIMSFTKLP--STPTEGLKSSSALTFCNPCMLNTRSVDSPATI 422

Query: 429 QLQSLELCRVH--DTPLAVSLCCQRLPSINL--CRKTPD 449
               +   RV    + L   L C   PS  L  CR + D
Sbjct: 423 SKAEIRRNRVAAVSSELVCPLTCSPFPSSALPTCRCSDD 458

BLAST of LsiUNG001000 vs. TrEMBL
Match: A0A0A0LWB1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G294100 PE=4 SV=1)

HSP 1 Score: 880.6 bits (2274), Expect = 8.7e-253
Identity = 435/470 (92.55%), Postives = 443/470 (94.26%), Query Frame = 1

Query: 1   MKLLSAVVNSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60
           MK LSAVV+SFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA
Sbjct: 1   MKFLSAVVHSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60

Query: 61  HGHHEIVYLLLQSGVDINLRNYRGQTALMQACQHGRWEVVLILVLFGANVHKADYLNGGT 120
           HGHHEIVYLLL SGVDINLRNYRGQTALMQACQHG WEVVLILVLFGANVHKADYLNG T
Sbjct: 61  HGHHEIVYLLLHSGVDINLRNYRGQTALMQACQHGHWEVVLILVLFGANVHKADYLNGWT 120

Query: 121 ALHLAATNGHSRCIRLLLADYIPSIPNFREIMS-QLTDNEESISEFDHRALSQIINQTAD 180
           ALHLAATNGHSRCIRLLLADYIPSIPNFREIMS QLTDNEESISEFDHRALSQIINQ AD
Sbjct: 121 ALHLAATNGHSRCIRLLLADYIPSIPNFREIMSRQLTDNEESISEFDHRALSQIINQKAD 180

Query: 181 GGITALHMAALNGQVESVQLLLEFGASVSEVTLGDGTVIDLIGAGSTALHYAACGGNAKC 240
           GGITALHMAALNGQVESVQLLLEFGASVSEVT+GDGTVIDLIGAGSTALHYAACGGNAKC
Sbjct: 181 GGITALHMAALNGQVESVQLLLEFGASVSEVTVGDGTVIDLIGAGSTALHYAACGGNAKC 240

Query: 241 CQLLIARGASLTAENENGWTPLMVARSWNKNWLEDILTAEPVDRPKVIPSRYISLPLMSI 300
           CQLLIARGASLT ENENGWTPLMV+RSWNKNWLEDILT EPV+RPK+IPSRYISLPLMSI
Sbjct: 241 CQLLIARGASLTTENENGWTPLMVSRSWNKNWLEDILTTEPVERPKLIPSRYISLPLMSI 300

Query: 301 VQIAREYGWTITSAPGCQDPCVVCLERKCTVAAEGCGHEFCTRCALYLCSTNCGTTISRG 360
           VQIAREYGWT  SAPGCQDPCVVCLERKCTVAAEGC HEFCTRCALYLCSTNCGTTISRG
Sbjct: 301 VQIAREYGWTSKSAPGCQDPCVVCLERKCTVAAEGCDHEFCTRCALYLCSTNCGTTISRG 360

Query: 361 PPGSVSCPLCRNGIVSFVKLPGKALVKEIARTSLSLSMCQCSHETPSKPTPLTTQLQSLE 420
           PPGSVSCPLCRNGIVSF KL  KAL KEIART LS  + QCS ETPSKPTPLTTQL+SLE
Sbjct: 361 PPGSVSCPLCRNGIVSFNKLRSKALAKEIARTRLSFPIRQCSQETPSKPTPLTTQLRSLE 420

Query: 421 LCRVHDTPLAVSLCCQRLPSINLCRKTPDICHSLVPRCANRNLQNHLARC 470
           LCRVHDTPL +  CC RLPSINLCRKTPDICHSLVPRCAN N QNHL RC
Sbjct: 421 LCRVHDTPLGIYFCCHRLPSINLCRKTPDICHSLVPRCANGNFQNHLGRC 470

BLAST of LsiUNG001000 vs. TrEMBL
Match: D7U1L4_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_11s0037g01410 PE=4 SV=1)

HSP 1 Score: 667.9 bits (1722), Expect = 8.9e-189
Identity = 346/478 (72.38%), Postives = 387/478 (80.96%), Query Frame = 1

Query: 1   MKLLSAVVNSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60
           M+ LS V NSFGCSASGERLVSAARDGD QEA+ALLEYNPRLARYSTFGVRNSPLHYSAA
Sbjct: 1   MRFLSIVGNSFGCSASGERLVSAARDGDLQEAKALLEYNPRLARYSTFGVRNSPLHYSAA 60

Query: 61  HGHHEIVYLLLQSGVDINLRNYRGQTALMQACQHGRWEVVLILVLFGANVHKADYLNGGT 120
            GHHEIV LLL SGVDINLRNYRGQTALMQACQHG WEVV  LVLF AN+H+ADYLNGGT
Sbjct: 61  QGHHEIVSLLLVSGVDINLRNYRGQTALMQACQHGHWEVVQTLVLFRANIHRADYLNGGT 120

Query: 121 ALHLAATNGHSRCIRLLLADYIPSIPNFREIMSQLTDNEESISEFDHRALSQIINQTADG 180
           ALHLAA NGHSRCIRLLLADYIPSIP+   I+ + ++ EESISEFD  AL Q+IN+ ADG
Sbjct: 121 ALHLAALNGHSRCIRLLLADYIPSIPDLWNILRKRSNREESISEFDEGALCQVINRPADG 180

Query: 181 GITALHMAALNGQVESVQLLLEFGASVSEVTLGDGTVIDLIGAGSTALHYAACGGNAKCC 240
           GITALHMAALNG VE++QLLL+ GAS+SEVT+ DGT IDLIGAGST LHYAACGGNA+CC
Sbjct: 181 GITALHMAALNGHVETLQLLLDLGASLSEVTVEDGTTIDLIGAGSTPLHYAACGGNAQCC 240

Query: 241 QLLIARGASLTAENENGWTPLMVARSWNKNWLEDILTAEPVDRPKVIPSRYISLPLMSIV 300
           Q+LIARGA L AEN NGWTPLMVARSW++ WLE+IL+ +P  + + I S Y+SLPLMSIV
Sbjct: 241 QILIARGAYLAAENANGWTPLMVARSWHRGWLEEILSTQPEVQSRTISSSYLSLPLMSIV 300

Query: 301 QIAREYGW-TITSAPGCQDPCVVCLERKCTVAAEGCGHEFCTRCALYLCSTNCGTTISRG 360
           +IARE GW    S P CQDPCVVCLERKCTVAA+GC HEFCT+CALYLCS N  +T+S+ 
Sbjct: 301 KIARECGWRNNDSLPTCQDPCVVCLERKCTVAAQGCDHEFCTKCALYLCSMNSSSTVSQC 360

Query: 361 PPGSVSCPLCRNGIVSFVKLPG-KALVKEIARTSLSLSMCQCSHETPSKPTPLTTQLQSL 420
           P GS++CPLCRNGIVSFVKLPG K LVKEIARTSLSLS C CS E P +PT LTT     
Sbjct: 361 PLGSIACPLCRNGIVSFVKLPGTKPLVKEIARTSLSLSFCTCSGEGP-EPTSLTTPFCKP 420

Query: 421 ELCRVHDTPLAV---SLCCQRLPSI----NLCRKTPDICHSLVPRCANRNLQNHLARC 470
           +      +PL     SL CQR  S+    +LC   PD   SLVP   +RNL+NHLARC
Sbjct: 421 DFHCSRISPLGSSFRSLSCQRFSSVKLNSSLCMGAPDTSPSLVPCTVDRNLRNHLARC 477

BLAST of LsiUNG001000 vs. TrEMBL
Match: A0A061GQ03_THECC (XB3 in isoform 1 OS=Theobroma cacao GN=TCM_038574 PE=4 SV=1)

HSP 1 Score: 663.3 bits (1710), Expect = 2.2e-187
Identity = 338/478 (70.71%), Postives = 390/478 (81.59%), Query Frame = 1

Query: 1   MKLLSAVVNSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60
           M+ LS V NSFGCSASGERLVSAARDGD QEA+ALLEYNPRLARYSTFGVRNSPLHYSAA
Sbjct: 1   MRFLSLVGNSFGCSASGERLVSAARDGDLQEAKALLEYNPRLARYSTFGVRNSPLHYSAA 60

Query: 61  HGHHEIVYLLLQSGVDINLRNYRGQTALMQACQHGRWEVVLILVLFGANVHKADYLNGGT 120
            GH EIV LLL+SGVDINLRNYRGQTALMQACQ+G WEVV  L+LFGAN+H+ADYLNGGT
Sbjct: 61  QGHQEIVSLLLESGVDINLRNYRGQTALMQACQYGHWEVVQTLILFGANIHRADYLNGGT 120

Query: 121 ALHLAATNGHSRCIRLLLADYIPSIPNFREIMSQLTDNEESISEFDHRALSQIINQTADG 180
           ALHLAA NGHSRCIRLLL+DYIPSIPN   I+ + + N+ESI+E D  AL ++IN+ ADG
Sbjct: 121 ALHLAALNGHSRCIRLLLSDYIPSIPNCWNILRKRSKNKESIAELDECALREVINRPADG 180

Query: 181 GITALHMAALNGQVESVQLLLEFGASVSEVTLGDGTVIDLIGAGSTALHYAACGGNAKCC 240
           G+TALHMAALNG V+SVQLLL+ GASV+EVT+ DGT IDLIGAGSTALHYAACGGNA+CC
Sbjct: 181 GVTALHMAALNGHVDSVQLLLDLGASVTEVTMEDGTTIDLIGAGSTALHYAACGGNAQCC 240

Query: 241 QLLIARGASLTAENENGWTPLMVARSWNKNWLEDILTAEPVDRPKVIPSRYISLPLMSIV 300
           Q+LIARGAS+TA N NGWT LMVARSW KNWL++IL+ +P  + +V+PS ++SLPLMSIV
Sbjct: 241 QILIARGASVTATNANGWTALMVARSWRKNWLDEILSTQPESQSQVLPSPFLSLPLMSIV 300

Query: 301 QIAREYGW-TITSAPGCQDPCVVCLERKCTVAAEGCGHEFCTRCALYLCSTNCGTTISRG 360
           +IARE GW T  S P CQDPC VCLERKCTVAAEGC HEFCT+CALYLCSTN  + +++G
Sbjct: 301 KIARECGWRTGDSLPSCQDPCAVCLERKCTVAAEGCDHEFCTQCALYLCSTNNTSNVAQG 360

Query: 361 PPGSVSCPLCRNGIVSFVKLPG-KALVKEIARTSLSLSMCQCSHETPSKPTPLTTQLQSL 420
           P GS++CPLCR+GIVSFVKLPG K  +K +ARTSLSLS C CS E P + T +TT L   
Sbjct: 361 PTGSIACPLCRHGIVSFVKLPGTKPTIKAVARTSLSLSFCTCSSEIP-ESTSMTTPLCKP 420

Query: 421 ELCRVHDTPLAVS---LCCQRLPSI----NLCRKTPDICHSLVPRCANRNLQNHLARC 470
           E+     +PL  S   L CQ  PS+    +LC   P+   SLVP  A+RNLQNHL RC
Sbjct: 421 EVHCTRISPLGSSFRNLSCQSFPSMKIHSSLCMGAPNTSPSLVPCPADRNLQNHLVRC 477

BLAST of LsiUNG001000 vs. TrEMBL
Match: M5XN89_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa004480mg PE=4 SV=1)

HSP 1 Score: 656.8 bits (1693), Expect = 2.0e-185
Identity = 338/477 (70.86%), Postives = 382/477 (80.08%), Query Frame = 1

Query: 1   MKLLSAVVNSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60
           MK LS + NSFGCSASGERLVSAARDGD QEA+ALLE NPRLARYSTFGVRNSPLHYSAA
Sbjct: 1   MKFLSVMGNSFGCSASGERLVSAARDGDLQEAKALLECNPRLARYSTFGVRNSPLHYSAA 60

Query: 61  HGHHEIVYLLLQSGVDINLRNYRGQTALMQACQHGRWEVVLILVLFGANVHKADYLNGGT 120
            GHHEIV LLL+SGVDINLRNYRGQTALMQACQHG WEVV  LVL  A +H+ADYLNGG 
Sbjct: 61  QGHHEIVSLLLESGVDINLRNYRGQTALMQACQHGHWEVVQTLVLSKATIHRADYLNGGI 120

Query: 121 ALHLAATNGHSRCIRLLLADYIPSIPNFREIMSQLTDNEESISEFDHRALSQIINQTADG 180
           ALH AA NGHSRCIRLLL DYIPS P+F   +   + NEESI+EF+H  L QIIN+ ADG
Sbjct: 121 ALHFAALNGHSRCIRLLLVDYIPSTPDFWSTLRNASSNEESIAEFNHDVLVQIINRPADG 180

Query: 181 GITALHMAALNGQVESVQLLLEFGASVSEVTLGDGTVIDLIGAGSTALHYAACGGNAKCC 240
           GITALHMAALNG VESVQLLL+ GASV +VT+ DGT IDLIGAGSTALHYAACGGNA+CC
Sbjct: 181 GITALHMAALNGHVESVQLLLDLGASVFKVTVQDGTTIDLIGAGSTALHYAACGGNAQCC 240

Query: 241 QLLIARGASLTAENENGWTPLMVARSWNKNWLEDILTAEPVDRPKVIPSRYISLPLMSIV 300
           Q+LI+RGASL AEN NGWTPLMVARSW+++WLE+IL+ E   + +++PS Y+SLPLMSIV
Sbjct: 241 QILISRGASLNAENANGWTPLMVARSWHRDWLEEILSTEQQVQSQILPSPYLSLPLMSIV 300

Query: 301 QIAREYGW-TITSAPGCQDPCVVCLERKCTVAAEGCGHEFCTRCALYLCSTNCGTTISRG 360
           +IARE GW    S   CQDPCVVCLE++CTVAAEGC HEFCTRCALYLCSTNC TT+S G
Sbjct: 301 KIARECGWRNSDSLSTCQDPCVVCLEQQCTVAAEGCDHEFCTRCALYLCSTNCTTTVSHG 360

Query: 361 PPGSVSCPLCRNGIVSFVKLPG-KALVKEIARTSLSLSMCQCSHETPSKPTPLTTQLQSL 420
           PPGS++CPLCR+GIVSFVKL G +++VKE  RTSLSLS+C CS E P   + +TT L   
Sbjct: 361 PPGSIACPLCRSGIVSFVKLRGTRSVVKENPRTSLSLSLCSCSAEEPDSAS-ITTPLCKP 420

Query: 421 ELCRVHDTPLAV--SLCCQRLPSI----NLCRKTPDICHSLVPRCANRNLQNHLARC 470
           E      +PL    SL CQ+ PS     NLC   P+I   LVP   NR L+NHLARC
Sbjct: 421 EFRCSGKSPLGSFRSLSCQKFPSFKINYNLCMGAPNINPCLVPSSGNRKLRNHLARC 476

BLAST of LsiUNG001000 vs. TrEMBL
Match: A0A059CN80_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_C01246 PE=4 SV=1)

HSP 1 Score: 655.6 bits (1690), Expect = 4.6e-185
Identity = 338/479 (70.56%), Postives = 386/479 (80.58%), Query Frame = 1

Query: 1   MKLLSAVVNSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60
           M+LL+ V NSFGCSASGERLVSAARDGD QEA+ALLEYNPRL RYSTFGVRNSPLHYSAA
Sbjct: 1   MRLLNLVGNSFGCSASGERLVSAARDGDLQEAKALLEYNPRLVRYSTFGVRNSPLHYSAA 60

Query: 61  HGHHEIVYLLLQSGVDINLRNYRGQTALMQACQHGRWEVVLILVLFGANVHKADYLNGGT 120
            GHHEIV LLL+SGVDINLRNYRGQTALMQACQHG WEVV  L+LF AN+H++DYLNGGT
Sbjct: 61  QGHHEIVSLLLESGVDINLRNYRGQTALMQACQHGHWEVVQTLILFKANIHRSDYLNGGT 120

Query: 121 ALHLAATNGHSRCIRLLLADYIPSIPNFREIMSQLTDNEESISEFDHRALSQIINQTADG 180
           ALHLAA NGH+RCIRLLLADYIPS P+   I+ + +++EE I+EFD RALSQ+IN+ +DG
Sbjct: 121 ALHLAALNGHTRCIRLLLADYIPSKPDVWNILRRRSNSEEPIAEFDERALSQVINRPSDG 180

Query: 181 GITALHMAALNGQVESVQLLLEFGASVSEVTLGDGTVIDLIGAGSTALHYAACGGNAKCC 240
           GITALHMAALNG VESVQLLL  GASVSEVT+ DGT IDLIGAGST LHYAACGG+A+CC
Sbjct: 181 GITALHMAALNGHVESVQLLLNLGASVSEVTVEDGTTIDLIGAGSTPLHYAACGGSAQCC 240

Query: 241 QLLIARGASLTAENENGWTPLMVARSWNKNWLEDILTAEPVDRPKVIPSRYISLPLMSIV 300
           Q+LIA GASL+ EN NGWTPLMVARSW++NWLE+IL+  P  R +++PS YISLPLMSIV
Sbjct: 241 QILIANGASLSVENVNGWTPLMVARSWHRNWLEEILSTPPEGRIRILPSPYISLPLMSIV 300

Query: 301 QIAREYGWTITS-APGCQDPCVVCLERKCTVAAEGCGHEFCTRCALYLCSTNCGTTISRG 360
           +IARE GW  +  +P CQDPCVVCLERKCTVAA+GC HEFCTRCALYLCST+  +T+  G
Sbjct: 301 RIARECGWRGSDLSPACQDPCVVCLERKCTVAAKGCEHEFCTRCALYLCSTSSSSTV-HG 360

Query: 361 PPGSVSCPLCRNGIVSFVKLPGKALV--KEIARTSLSLSMCQCSHETPSKPTPLTTQLQS 420
           PPGS++CPLCR+GIVSF KLPG   V  KEIARTSLSL+ C CS E  S+  PLTT L  
Sbjct: 361 PPGSIACPLCRHGIVSFAKLPGTRPVVNKEIARTSLSLTFCTCSGEA-SETAPLTTPLCK 420

Query: 421 LELCRVHDTPLAV---SLCCQRLPSI----NLCRKTPDICHSLVPRCANRNLQNHLARC 470
                   +PL     SL CQ+ PS+    +LC  TPD   SLVP    RNL+  L RC
Sbjct: 421 PAFQCTRISPLGSSFRSLSCQKFPSMKINTSLCMGTPDTSPSLVPCSVKRNLRTQLVRC 477

BLAST of LsiUNG001000 vs. TAIR10
Match: AT5G57740.1 (AT5G57740.1 XB3 ortholog 2 in Arabidopsis thaliana)

HSP 1 Score: 558.1 bits (1437), Expect = 5.0e-159
Identity = 279/411 (67.88%), Postives = 327/411 (79.56%), Query Frame = 1

Query: 1   MKLLSAVVNSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60
           M+ LS V NSFGCSASGERLVSAARDGD QEA+ALL+YNPRLARYSTFGVRNSPLHYSAA
Sbjct: 1   MRFLSLVGNSFGCSASGERLVSAARDGDLQEAKALLDYNPRLARYSTFGVRNSPLHYSAA 60

Query: 61  HGHHEIVYLLLQSGVDINLRNYRGQTALMQACQHGRWEVVLILVLFGANVHKADYLNGGT 120
            GHHEIV LL++SGVDINLRNYRGQTALMQACQHG WEVVLIL+LFGAN+H++DYLNGGT
Sbjct: 61  QGHHEIVSLLVESGVDINLRNYRGQTALMQACQHGHWEVVLILILFGANIHRSDYLNGGT 120

Query: 121 ALHLAATNGHSRCIRLLLADYIPSIPNFREIMSQLTDNEESISEFDHRALSQIINQTADG 180
           ALHLAA NGH RCIR+LL++YIPS+PN     S L + + S++ FD   L ++IN+ ADG
Sbjct: 121 ALHLAALNGHPRCIRILLSEYIPSVPN---CWSLLKNKKTSVAGFDSSVLHEVINRAADG 180

Query: 181 GITALHMAALNGQVESVQLLLEFGASVSEVTLGDGTVIDLIGAGSTALHYAACGGNAKCC 240
           GIT LH+AALNG +E+VQLLL+ GASV++VT+ DGT IDLIGAGSTALHYA+CGGN +CC
Sbjct: 181 GITPLHVAALNGHIETVQLLLDLGASVTQVTVEDGTTIDLIGAGSTALHYASCGGNTQCC 240

Query: 241 QLLIARGASLTAENENGWTPLMVARSWNKNWLEDIL---TAEPVDRPKVIPSRYISLPLM 300
           QLLI++GA L A N NGWTP+MVARSW++NWLE+IL   T +P      +PS ++ LPLM
Sbjct: 241 QLLISKGACLAAVNSNGWTPMMVARSWHRNWLEEILNPTTEQPQLHLPNVPSPFLCLPLM 300

Query: 301 SIVQIAREYGWTITSA-PGCQDPCVVCLERKCTVAAEGCGHEFCTRCALYLCSTN-CGTT 360
           SIV IA+E GW        C+DPC VCLERKCTVAA+GC HEFCT CALYL +T+   + 
Sbjct: 301 SIVNIAQECGWRENDCLTPCRDPCAVCLERKCTVAADGCAHEFCTNCALYLSTTSITSSK 360

Query: 361 ISRGPPGSVSCPLCRNGIVSFVKLP---GKALVKEIARTSLSLSMCQCSHE 404
            S   PGSV CPLCRNGIVSF KLP           +RTS+SLS C CS +
Sbjct: 361 TSNVTPGSVPCPLCRNGIVSFTKLPHTTATTRTSTSSRTSISLSFCTCSSD 408

BLAST of LsiUNG001000 vs. TAIR10
Match: AT5G07270.1 (AT5G07270.1 XB3 ortholog 3 in Arabidopsis thaliana)

HSP 1 Score: 443.7 bits (1140), Expect = 1.4e-124
Identity = 245/470 (52.13%), Postives = 315/470 (67.02%), Query Frame = 1

Query: 9   NSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAAHGHHEIVY 68
           NSFGCSASGERLVSAARDGDF EA+ LL+ NP LA+YSTFG  NSPLH++AA GH+EIV 
Sbjct: 3   NSFGCSASGERLVSAARDGDFVEAKMLLDCNPCLAKYSTFGGLNSPLHFAAAKGHNEIVG 62

Query: 69  LLLQSGVDINLRNYRGQTALMQACQHGRWEVVLILVLFGANVHKADYLNGGTALHLAATN 128
           LLL++G D+N RNY GQTALMQAC++G WEVV  L+LF  NV +ADYL G TALH AA N
Sbjct: 63  LLLENGADVNSRNYCGQTALMQACRYGHWEVVQTLLLFRCNVTRADYLAGRTALHFAAVN 122

Query: 129 GHSRCIRLLLADYIPSIPNFREIMSQL--TDNEESISEFDHRALSQIINQTADGGITALH 188
           GH+RCIRL+LAD++PS     + ++ L  T    + ++ +  ALS+ +N+ ADGGITALH
Sbjct: 123 GHARCIRLVLADFLPS-----DKLNSLPETGVVTAKNKSEQSALSKFVNKAADGGITALH 182

Query: 189 MAALNGQVESVQLLLEFGASVSEVTLGDGTVIDLIGAGSTALHYAACGGNAKCCQLLIAR 248
           MAALNG  + VQLLL+  A+VS VT   GT +D+IGAGST LHYAACGGN KCCQ+L+AR
Sbjct: 183 MAALNGLFDCVQLLLDLEANVSAVTFHYGTSMDMIGAGSTPLHYAACGGNLKCCQILLAR 242

Query: 249 GASLTAENENGWTPLMVARSWNKNWLEDILTA-EPVDRPKVIPSRYISLPLMSIVQIARE 308
           GA     N NGW P+ +AR W+++WLE +L+    V  P    S Y+SLPL+SI+ IARE
Sbjct: 243 GARKMTLNCNGWLPIDIARMWSRHWLEPLLSPNSDVVIPAFPHSNYLSLPLLSILNIARE 302

Query: 309 YGWTITSAPGCQDPCVVCLERKCTVAAEGCGHEFCTRCALYLCSTNCGTTISRGPPGSVS 368
           +G    +     D C VCLER CTVAAEGC H+ C RCALYLCS++   +++ GPPGS+ 
Sbjct: 303 FGLQSATIGDEVDICAVCLERTCTVAAEGCEHQLCVRCALYLCSSSNVPSVTVGPPGSIP 362

Query: 369 CPLCRNGIVSFVKLPGKALVKEIARTSLSLSMC-QCSHET-----PSKPT-PLTTQLQSL 428
           CPLCR+GI +F +LP  +L +E+ +  +SL  C  C   T      S PT P T Q  S 
Sbjct: 363 CPLCRHGITAFKRLP-SSLTREM-KLPMSLGFCAPCMLHTGDTTDQSSPTCPPTEQRSSK 422

Query: 429 ELCRVHDTPLAVSLCCQRLPSIN--LCRKTPDICHSLVPRCANRNLQNHL 467
                  + +   + C   PS+N  +C      C +       R+ + H+
Sbjct: 423 TRAASVSSDIFCPVTCSPFPSVNIPMCTCNEGTCPNFETHGTERHSEEHV 465

BLAST of LsiUNG001000 vs. TAIR10
Match: AT2G28840.1 (AT2G28840.1 XB3 ortholog 1 in Arabidopsis thaliana)

HSP 1 Score: 134.0 bits (336), Expect = 2.3e-31
Identity = 119/401 (29.68%), Postives = 177/401 (44.14%), Query Frame = 1

Query: 10  SFGCSASGER-LVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAAHGHHEIVY 69
           S  C +  E  + ++ + GD    + ++   P L   +T   R+S LH +AA+G  EI+ 
Sbjct: 4   SMSCGSRPEHGIFASVQCGDIITIRRVMATEPSLLNQTTPYDRHSVLHVAAANGQIEILS 63

Query: 70  LLLQSGVDINLRNYRGQTALMQACQHGRWEVVLILVLFGANVHKADYLNGGTALHLAATN 129
           LLL+   + +L N   QT LM A  +GR   V  L   GAN+   D +N  T LH AA  
Sbjct: 64  LLLERFTNPDLLNRHKQTPLMLAAMYGRISCVKKLAEVGANILMFDSVNRRTCLHYAAYY 123

Query: 130 GHSRCIRLLLADYIPSIPNFREIMSQLTDNEESISEFDHRALSQIINQTADGGITALHMA 189
           GH+ C++ +L+                    +S     H   ++ +N   D G T LH+A
Sbjct: 124 GHANCVQAILS------------------AAQSSPVAVHWGYARFVNIRDDKGATPLHLA 183

Query: 190 ALNGQVESVQLLLEFGASVSEVTLGDGTVIDLIGAGSTALHYAACGGNAKCCQLLIARGA 249
           A   + E V +LL+ G+ V   T   G+       GST LH AA  G+  C + L+A GA
Sbjct: 184 ARQRRPECVNVLLDSGSLVCASTSVYGS------PGSTPLHLAARSGSIDCVRKLLAWGA 243

Query: 250 SLTAENENGWTPLMVARSWNKNWLEDIL---TAEPVDRPKVIPSRYIS--------LPLM 309
                + +G  P +VA          +L   +AEP+  P   P ++IS        L   
Sbjct: 244 DRLQRDASGRIPYVVAMKHKHGACGALLNPSSAEPLVWPS--PLKFISELNDEAKLLLEQ 303

Query: 310 SIVQIAREYGWTITS-------APGCQDP------------CVVCLERKCTVAAEGCGHE 369
           ++++  RE   TI         +P   D             C +C E+ CT+  + CGH+
Sbjct: 304 ALMEANREREKTILKGTAYSLPSPSFSDTDDNMSEVSDTELCCICFEQVCTIEVKDCGHQ 363

Query: 370 FCTRCALYLCSTN--CGTTISRGPPGSVSCPLCRNGIVSFV 378
            C +C L LC  N    TT +  PP    CP CR+ I   V
Sbjct: 364 MCAQCTLALCCHNKPNPTTSTVTPP---VCPFCRSTIACLV 375

BLAST of LsiUNG001000 vs. TAIR10
Match: AT3G04710.1 (AT3G04710.1 ankyrin repeat family protein)

HSP 1 Score: 67.8 bits (164), Expect = 2.1e-11
Identity = 66/232 (28.45%), Postives = 107/232 (46.12%), Query Frame = 1

Query: 51  RNSPLHYSAAHGHHEIV-YLLLQSGVDINLRNYRGQTALMQACQHGRWEVVLILVLFGAN 110
           +   LH++A  G  EI  YLL +  ++ + ++  G T L+ A + G+ E V  L+  GA+
Sbjct: 55  KRGALHFAAREGQTEICRYLLEELKLNADAKDETGDTPLVHAARQGQIETVKYLLEQGAD 114

Query: 111 VHKADYLNGGTALHLAATNGHSRCIRLLLADYIP----SIPNFREIMSQLTDNEESISEF 170
            + A  L G TALH AA  G    ++ LL+  +P    S      I +   D + ++   
Sbjct: 115 PNIASEL-GATALHHAAGTGEIELLKELLSRGVPVDSESESGTPLIWAAGHDQKNAVEVL 174

Query: 171 -DHRALSQIINQTADGGITALHMAALNGQVESVQLLLEFGASVSEVTLGDGTVIDLIGAG 230
            +H A     N   +  IT L  A   G +  ++LL++ GA  +           +   G
Sbjct: 175 LEHNANP---NAETEDNITPLLSAVAAGSLSCLELLVKAGAKAN-----------VFAGG 234

Query: 231 STALHYAACGGNAKCCQLLIARGASLTAENENGWTPLMVARSWNKNWLEDIL 277
           +T LH AA  GN +    L+  GA    ++E G  PL VA + +   + +IL
Sbjct: 235 ATPLHIAADIGNLELINCLLKAGADPNQKDEEGNRPLEVAAARDNRKVVEIL 271

BLAST of LsiUNG001000 vs. TAIR10
Match: AT5G37500.1 (AT5G37500.1 gated outwardly-rectifying K+ channel)

HSP 1 Score: 67.8 bits (164), Expect = 2.1e-11
Identity = 67/224 (29.91%), Postives = 99/224 (44.20%), Query Frame = 1

Query: 22  SAARDGDFQEAQALLEY--NPRLARYSTFGVRNSPLHYSAAHGHHEIVYLLLQSGVDINL 81
           SAA  GDF + ++L+    +P    Y       SPLH +A  G+ +I   L+Q GVD+NL
Sbjct: 537 SAAFQGDFYQLKSLIRSGADPNKTDYDG----RSPLHLAACRGYEDITLFLIQEGVDVNL 596

Query: 82  RNYRGQTALMQACQHGRWEVVLILVLFGANVHKADYLNGGTALHLAATNGHSRCIRLLLA 141
           ++  G T L +A + G+  V+ +LV  GA+ +  D    G  L      G S  ++ LL+
Sbjct: 597 KDKFGHTPLFEAVKAGQEGVIGLLVKEGASFNLED---SGNFLCTTVAKGDSDFLKRLLS 656

Query: 142 DYIPSIPNFREIMSQLTDNEESISEFDHRALSQIINQTADGGITALHMAALNGQVESVQL 201
                        S +  N E   ++DHR              T LH+AA  G     ++
Sbjct: 657 -------------SGMNPNSE---DYDHR--------------TPLHVAASEGLFLMAKM 713

Query: 202 LLEFGASVSEVTLGDGTVIDLIGAGSTALHYAACGGNAKCCQLL 244
           L+E GAS          VI     G++ L  A   GN K  +LL
Sbjct: 717 LVEAGAS----------VISKDRWGNSPLDEARLCGNKKLIKLL 713

BLAST of LsiUNG001000 vs. NCBI nr
Match: gi|659086179|ref|XP_008443799.1| (PREDICTED: E3 ubiquitin-protein ligase XBAT32 [Cucumis melo])

HSP 1 Score: 883.2 bits (2281), Expect = 1.9e-253
Identity = 433/470 (92.13%), Postives = 447/470 (95.11%), Query Frame = 1

Query: 1   MKLLSAVVNSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60
           MK LSAVV+SFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA
Sbjct: 1   MKFLSAVVHSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60

Query: 61  HGHHEIVYLLLQSGVDINLRNYRGQTALMQACQHGRWEVVLILVLFGANVHKADYLNGGT 120
           HGHHEIVYLLL SGVDINLRNYRGQTALMQACQHG WEVVLILVLFGANVHKADYLNG T
Sbjct: 61  HGHHEIVYLLLHSGVDINLRNYRGQTALMQACQHGHWEVVLILVLFGANVHKADYLNGWT 120

Query: 121 ALHLAATNGHSRCIRLLLADYIPSIPNFREIMS-QLTDNEESISEFDHRALSQIINQTAD 180
           ALHLAATNGHSRCIRLLLADYIPSIPNFREIMS Q TD+EESISEFDHRALSQIINQ AD
Sbjct: 121 ALHLAATNGHSRCIRLLLADYIPSIPNFREIMSRQSTDSEESISEFDHRALSQIINQKAD 180

Query: 181 GGITALHMAALNGQVESVQLLLEFGASVSEVTLGDGTVIDLIGAGSTALHYAACGGNAKC 240
           GGITALHMAALNGQVESVQLLLEFGASVSEVT+GDGTVIDLIGAGSTALHYAACGGNAKC
Sbjct: 181 GGITALHMAALNGQVESVQLLLEFGASVSEVTVGDGTVIDLIGAGSTALHYAACGGNAKC 240

Query: 241 CQLLIARGASLTAENENGWTPLMVARSWNKNWLEDILTAEPVDRPKVIPSRYISLPLMSI 300
           CQLLIARGASLT ENENGWTPLMV+RSWNK+WLEDILT EPV+RPK+IPSRYISLPLMSI
Sbjct: 241 CQLLIARGASLTTENENGWTPLMVSRSWNKSWLEDILTTEPVERPKLIPSRYISLPLMSI 300

Query: 301 VQIAREYGWTITSAPGCQDPCVVCLERKCTVAAEGCGHEFCTRCALYLCSTNCGTTISRG 360
           VQIAREYGW+  SAPGCQDPCVVCLERKCTVAA GC HEFCTRCALYLCSTNCGTTISRG
Sbjct: 301 VQIAREYGWSSKSAPGCQDPCVVCLERKCTVAAGGCDHEFCTRCALYLCSTNCGTTISRG 360

Query: 361 PPGSVSCPLCRNGIVSFVKLPGKALVKEIARTSLSLSMCQCSHETPSKPTPLTTQLQSLE 420
           PPGSVSCPLCRNGIVSFVKLPGKAL KEIARTSLS  +CQCSHETP KPTPLTTQL+SLE
Sbjct: 361 PPGSVSCPLCRNGIVSFVKLPGKALAKEIARTSLSFPICQCSHETPPKPTPLTTQLRSLE 420

Query: 421 LCRVHDTPLAVSLCCQRLPSINLCRKTPDICHSLVPRCANRNLQNHLARC 470
           LCRVHDTPL +  CC +LPSI+LCRK PDICHSLVPRCAN NLQNHL+RC
Sbjct: 421 LCRVHDTPLGIYFCCHKLPSISLCRKAPDICHSLVPRCANGNLQNHLSRC 470

BLAST of LsiUNG001000 vs. NCBI nr
Match: gi|449464328|ref|XP_004149881.1| (PREDICTED: E3 ubiquitin-protein ligase XBAT32 isoform X1 [Cucumis sativus])

HSP 1 Score: 880.6 bits (2274), Expect = 1.2e-252
Identity = 435/470 (92.55%), Postives = 443/470 (94.26%), Query Frame = 1

Query: 1   MKLLSAVVNSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60
           MK LSAVV+SFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA
Sbjct: 1   MKFLSAVVHSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60

Query: 61  HGHHEIVYLLLQSGVDINLRNYRGQTALMQACQHGRWEVVLILVLFGANVHKADYLNGGT 120
           HGHHEIVYLLL SGVDINLRNYRGQTALMQACQHG WEVVLILVLFGANVHKADYLNG T
Sbjct: 61  HGHHEIVYLLLHSGVDINLRNYRGQTALMQACQHGHWEVVLILVLFGANVHKADYLNGWT 120

Query: 121 ALHLAATNGHSRCIRLLLADYIPSIPNFREIMS-QLTDNEESISEFDHRALSQIINQTAD 180
           ALHLAATNGHSRCIRLLLADYIPSIPNFREIMS QLTDNEESISEFDHRALSQIINQ AD
Sbjct: 121 ALHLAATNGHSRCIRLLLADYIPSIPNFREIMSRQLTDNEESISEFDHRALSQIINQKAD 180

Query: 181 GGITALHMAALNGQVESVQLLLEFGASVSEVTLGDGTVIDLIGAGSTALHYAACGGNAKC 240
           GGITALHMAALNGQVESVQLLLEFGASVSEVT+GDGTVIDLIGAGSTALHYAACGGNAKC
Sbjct: 181 GGITALHMAALNGQVESVQLLLEFGASVSEVTVGDGTVIDLIGAGSTALHYAACGGNAKC 240

Query: 241 CQLLIARGASLTAENENGWTPLMVARSWNKNWLEDILTAEPVDRPKVIPSRYISLPLMSI 300
           CQLLIARGASLT ENENGWTPLMV+RSWNKNWLEDILT EPV+RPK+IPSRYISLPLMSI
Sbjct: 241 CQLLIARGASLTTENENGWTPLMVSRSWNKNWLEDILTTEPVERPKLIPSRYISLPLMSI 300

Query: 301 VQIAREYGWTITSAPGCQDPCVVCLERKCTVAAEGCGHEFCTRCALYLCSTNCGTTISRG 360
           VQIAREYGWT  SAPGCQDPCVVCLERKCTVAAEGC HEFCTRCALYLCSTNCGTTISRG
Sbjct: 301 VQIAREYGWTSKSAPGCQDPCVVCLERKCTVAAEGCDHEFCTRCALYLCSTNCGTTISRG 360

Query: 361 PPGSVSCPLCRNGIVSFVKLPGKALVKEIARTSLSLSMCQCSHETPSKPTPLTTQLQSLE 420
           PPGSVSCPLCRNGIVSF KL  KAL KEIART LS  + QCS ETPSKPTPLTTQL+SLE
Sbjct: 361 PPGSVSCPLCRNGIVSFNKLRSKALAKEIARTRLSFPIRQCSQETPSKPTPLTTQLRSLE 420

Query: 421 LCRVHDTPLAVSLCCQRLPSINLCRKTPDICHSLVPRCANRNLQNHLARC 470
           LCRVHDTPL +  CC RLPSINLCRKTPDICHSLVPRCAN N QNHL RC
Sbjct: 421 LCRVHDTPLGIYFCCHRLPSINLCRKTPDICHSLVPRCANGNFQNHLGRC 470

BLAST of LsiUNG001000 vs. NCBI nr
Match: gi|1009108629|ref|XP_015885660.1| (PREDICTED: E3 ubiquitin-protein ligase XBAT32 isoform X1 [Ziziphus jujuba])

HSP 1 Score: 678.7 bits (1750), Expect = 7.2e-192
Identity = 346/477 (72.54%), Postives = 392/477 (82.18%), Query Frame = 1

Query: 1   MKLLSAVVNSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60
           MK LS V NSFGCSASGERLVSAARDGD QEA+ALLEYNPRL RYSTFGVRNSPLHYSAA
Sbjct: 1   MKFLSIVGNSFGCSASGERLVSAARDGDLQEAKALLEYNPRLVRYSTFGVRNSPLHYSAA 60

Query: 61  HGHHEIVYLLLQSGVDINLRNYRGQTALMQACQHGRWEVVLILVLFGANVHKADYLNGGT 120
            GHHEIV LLL+SGVDINLRNYRGQTALMQACQHG WEVV  LVLFGAN+H+ADYLNGGT
Sbjct: 61  QGHHEIVSLLLESGVDINLRNYRGQTALMQACQHGHWEVVQNLVLFGANIHRADYLNGGT 120

Query: 121 ALHLAATNGHSRCIRLLLADYIPSIPNFREIMSQLTDNEESISEFDHRALSQIINQTADG 180
           ALHLAA NGHSRCIRLLLADYIPS+P+F ++++  +DN+ S SEFDH AL Q+IN+ ADG
Sbjct: 121 ALHLAALNGHSRCIRLLLADYIPSLPDFWQMLNT-SDNDVSSSEFDHSALRQVINKPADG 180

Query: 181 GITALHMAALNGQVESVQLLLEFGASVSEVTLGDGTVIDLIGAGSTALHYAACGGNAKCC 240
           GITALHMAALNG VESVQLLL+ GASV+EVT+ DGT IDLIGAGSTALHYAACGGNA+CC
Sbjct: 181 GITALHMAALNGHVESVQLLLDLGASVTEVTVEDGTTIDLIGAGSTALHYAACGGNAQCC 240

Query: 241 QLLIARGASLTAENENGWTPLMVARSWNKNWLEDILTAEPVDRPKVIPSRYISLPLMSIV 300
           Q+LIA+G SLTAEN NGW  LMVARSW++ WLE+ILT +   + + +PS Y+SLPLMSI+
Sbjct: 241 QILIAKGTSLTAENANGWNALMVARSWHRTWLEEILTTQAEGQSQSLPSPYLSLPLMSII 300

Query: 301 QIAREYGWTITSAPGCQDPCVVCLERKCTVAAEGCGHEFCTRCALYLCSTNCGTTISRGP 360
           +IARE GW   S    QDPCVVCLER+CTVAAEGC HEFCTRCALYLCSTNC +T++ GP
Sbjct: 301 KIARECGWRNDSLSSSQDPCVVCLERQCTVAAEGCDHEFCTRCALYLCSTNCTSTVAHGP 360

Query: 361 PGSVSCPLCRNGIVSFVKLPG-KALVKEIARTSLSLSMCQCSHETPSKPTPLTTQLQSLE 420
           PGS++CPLCRNGIVSF  LPG K  +KEIARTSLSLS C CS E P +PTPLT   +  E
Sbjct: 361 PGSIACPLCRNGIVSFAMLPGTKPAIKEIARTSLSLSFCSCSGEGP-EPTPLTPFCKP-E 420

Query: 421 LCRVHDTPLAV---SLCCQRLPSI----NLCRKTPDICHSLVPRCANRNLQNHLARC 470
           L     +PL     SL CQR PS+    +LC + P+I  SL+P   +RNLQ HLARC
Sbjct: 421 LRCSRISPLGSSFRSLSCQRFPSVRINSSLCMRAPEISPSLIPCTVDRNLQRHLARC 474

BLAST of LsiUNG001000 vs. NCBI nr
Match: gi|225445704|ref|XP_002269428.1| (PREDICTED: probable E3 ubiquitin-protein ligase XBOS32 [Vitis vinifera])

HSP 1 Score: 667.9 bits (1722), Expect = 1.3e-188
Identity = 346/478 (72.38%), Postives = 387/478 (80.96%), Query Frame = 1

Query: 1   MKLLSAVVNSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60
           M+ LS V NSFGCSASGERLVSAARDGD QEA+ALLEYNPRLARYSTFGVRNSPLHYSAA
Sbjct: 1   MRFLSIVGNSFGCSASGERLVSAARDGDLQEAKALLEYNPRLARYSTFGVRNSPLHYSAA 60

Query: 61  HGHHEIVYLLLQSGVDINLRNYRGQTALMQACQHGRWEVVLILVLFGANVHKADYLNGGT 120
            GHHEIV LLL SGVDINLRNYRGQTALMQACQHG WEVV  LVLF AN+H+ADYLNGGT
Sbjct: 61  QGHHEIVSLLLVSGVDINLRNYRGQTALMQACQHGHWEVVQTLVLFRANIHRADYLNGGT 120

Query: 121 ALHLAATNGHSRCIRLLLADYIPSIPNFREIMSQLTDNEESISEFDHRALSQIINQTADG 180
           ALHLAA NGHSRCIRLLLADYIPSIP+   I+ + ++ EESISEFD  AL Q+IN+ ADG
Sbjct: 121 ALHLAALNGHSRCIRLLLADYIPSIPDLWNILRKRSNREESISEFDEGALCQVINRPADG 180

Query: 181 GITALHMAALNGQVESVQLLLEFGASVSEVTLGDGTVIDLIGAGSTALHYAACGGNAKCC 240
           GITALHMAALNG VE++QLLL+ GAS+SEVT+ DGT IDLIGAGST LHYAACGGNA+CC
Sbjct: 181 GITALHMAALNGHVETLQLLLDLGASLSEVTVEDGTTIDLIGAGSTPLHYAACGGNAQCC 240

Query: 241 QLLIARGASLTAENENGWTPLMVARSWNKNWLEDILTAEPVDRPKVIPSRYISLPLMSIV 300
           Q+LIARGA L AEN NGWTPLMVARSW++ WLE+IL+ +P  + + I S Y+SLPLMSIV
Sbjct: 241 QILIARGAYLAAENANGWTPLMVARSWHRGWLEEILSTQPEVQSRTISSSYLSLPLMSIV 300

Query: 301 QIAREYGW-TITSAPGCQDPCVVCLERKCTVAAEGCGHEFCTRCALYLCSTNCGTTISRG 360
           +IARE GW    S P CQDPCVVCLERKCTVAA+GC HEFCT+CALYLCS N  +T+S+ 
Sbjct: 301 KIARECGWRNNDSLPTCQDPCVVCLERKCTVAAQGCDHEFCTKCALYLCSMNSSSTVSQC 360

Query: 361 PPGSVSCPLCRNGIVSFVKLPG-KALVKEIARTSLSLSMCQCSHETPSKPTPLTTQLQSL 420
           P GS++CPLCRNGIVSFVKLPG K LVKEIARTSLSLS C CS E P +PT LTT     
Sbjct: 361 PLGSIACPLCRNGIVSFVKLPGTKPLVKEIARTSLSLSFCTCSGEGP-EPTSLTTPFCKP 420

Query: 421 ELCRVHDTPLAV---SLCCQRLPSI----NLCRKTPDICHSLVPRCANRNLQNHLARC 470
           +      +PL     SL CQR  S+    +LC   PD   SLVP   +RNL+NHLARC
Sbjct: 421 DFHCSRISPLGSSFRSLSCQRFSSVKLNSSLCMGAPDTSPSLVPCTVDRNLRNHLARC 477

BLAST of LsiUNG001000 vs. NCBI nr
Match: gi|590580142|ref|XP_007013987.1| (XB3 in isoform 1 [Theobroma cacao])

HSP 1 Score: 663.3 bits (1710), Expect = 3.1e-187
Identity = 338/478 (70.71%), Postives = 390/478 (81.59%), Query Frame = 1

Query: 1   MKLLSAVVNSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60
           M+ LS V NSFGCSASGERLVSAARDGD QEA+ALLEYNPRLARYSTFGVRNSPLHYSAA
Sbjct: 1   MRFLSLVGNSFGCSASGERLVSAARDGDLQEAKALLEYNPRLARYSTFGVRNSPLHYSAA 60

Query: 61  HGHHEIVYLLLQSGVDINLRNYRGQTALMQACQHGRWEVVLILVLFGANVHKADYLNGGT 120
            GH EIV LLL+SGVDINLRNYRGQTALMQACQ+G WEVV  L+LFGAN+H+ADYLNGGT
Sbjct: 61  QGHQEIVSLLLESGVDINLRNYRGQTALMQACQYGHWEVVQTLILFGANIHRADYLNGGT 120

Query: 121 ALHLAATNGHSRCIRLLLADYIPSIPNFREIMSQLTDNEESISEFDHRALSQIINQTADG 180
           ALHLAA NGHSRCIRLLL+DYIPSIPN   I+ + + N+ESI+E D  AL ++IN+ ADG
Sbjct: 121 ALHLAALNGHSRCIRLLLSDYIPSIPNCWNILRKRSKNKESIAELDECALREVINRPADG 180

Query: 181 GITALHMAALNGQVESVQLLLEFGASVSEVTLGDGTVIDLIGAGSTALHYAACGGNAKCC 240
           G+TALHMAALNG V+SVQLLL+ GASV+EVT+ DGT IDLIGAGSTALHYAACGGNA+CC
Sbjct: 181 GVTALHMAALNGHVDSVQLLLDLGASVTEVTMEDGTTIDLIGAGSTALHYAACGGNAQCC 240

Query: 241 QLLIARGASLTAENENGWTPLMVARSWNKNWLEDILTAEPVDRPKVIPSRYISLPLMSIV 300
           Q+LIARGAS+TA N NGWT LMVARSW KNWL++IL+ +P  + +V+PS ++SLPLMSIV
Sbjct: 241 QILIARGASVTATNANGWTALMVARSWRKNWLDEILSTQPESQSQVLPSPFLSLPLMSIV 300

Query: 301 QIAREYGW-TITSAPGCQDPCVVCLERKCTVAAEGCGHEFCTRCALYLCSTNCGTTISRG 360
           +IARE GW T  S P CQDPC VCLERKCTVAAEGC HEFCT+CALYLCSTN  + +++G
Sbjct: 301 KIARECGWRTGDSLPSCQDPCAVCLERKCTVAAEGCDHEFCTQCALYLCSTNNTSNVAQG 360

Query: 361 PPGSVSCPLCRNGIVSFVKLPG-KALVKEIARTSLSLSMCQCSHETPSKPTPLTTQLQSL 420
           P GS++CPLCR+GIVSFVKLPG K  +K +ARTSLSLS C CS E P + T +TT L   
Sbjct: 361 PTGSIACPLCRHGIVSFVKLPGTKPTIKAVARTSLSLSFCTCSSEIP-ESTSMTTPLCKP 420

Query: 421 ELCRVHDTPLAVS---LCCQRLPSI----NLCRKTPDICHSLVPRCANRNLQNHLARC 470
           E+     +PL  S   L CQ  PS+    +LC   P+   SLVP  A+RNLQNHL RC
Sbjct: 421 EVHCTRISPLGSSFRNLSCQSFPSMKIHSSLCMGAPNTSPSLVPCPADRNLQNHLVRC 477

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XB32_ORYSJ2.8e-15963.38Probable E3 ubiquitin-protein ligase XBOS32 OS=Oryza sativa subsp. japonica GN=X... [more]
XB32_ARATH8.9e-15867.88E3 ubiquitin-protein ligase XBAT32 OS=Arabidopsis thaliana GN=XBAT32 PE=1 SV=1[more]
XB35_ORYSJ1.1e-14763.48Probable E3 ubiquitin-protein ligase XBOS35 OS=Oryza sativa subsp. japonica GN=X... [more]
XB33_ARATH2.4e-12352.13E3 ubiquitin-protein ligase XBAT33 OS=Arabidopsis thaliana GN=XBAT33 PE=2 SV=1[more]
XB33_ORYSJ5.3e-11852.72Probable E3 ubiquitin-protein ligase XBOS33 OS=Oryza sativa subsp. japonica GN=X... [more]
Match NameE-valueIdentityDescription
A0A0A0LWB1_CUCSA8.7e-25392.55Uncharacterized protein OS=Cucumis sativus GN=Csa_1G294100 PE=4 SV=1[more]
D7U1L4_VITVI8.9e-18972.38Putative uncharacterized protein OS=Vitis vinifera GN=VIT_11s0037g01410 PE=4 SV=... [more]
A0A061GQ03_THECC2.2e-18770.71XB3 in isoform 1 OS=Theobroma cacao GN=TCM_038574 PE=4 SV=1[more]
M5XN89_PRUPE2.0e-18570.86Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa004480mg PE=4 SV=1[more]
A0A059CN80_EUCGR4.6e-18570.56Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_C01246 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G57740.15.0e-15967.88 XB3 ortholog 2 in Arabidopsis thaliana[more]
AT5G07270.11.4e-12452.13 XB3 ortholog 3 in Arabidopsis thaliana[more]
AT2G28840.12.3e-3129.68 XB3 ortholog 1 in Arabidopsis thaliana[more]
AT3G04710.12.1e-1128.45 ankyrin repeat family protein[more]
AT5G37500.12.1e-1129.91 gated outwardly-rectifying K+ channel[more]
Match NameE-valueIdentityDescription
gi|659086179|ref|XP_008443799.1|1.9e-25392.13PREDICTED: E3 ubiquitin-protein ligase XBAT32 [Cucumis melo][more]
gi|449464328|ref|XP_004149881.1|1.2e-25292.55PREDICTED: E3 ubiquitin-protein ligase XBAT32 isoform X1 [Cucumis sativus][more]
gi|1009108629|ref|XP_015885660.1|7.2e-19272.54PREDICTED: E3 ubiquitin-protein ligase XBAT32 isoform X1 [Ziziphus jujuba][more]
gi|225445704|ref|XP_002269428.1|1.3e-18872.38PREDICTED: probable E3 ubiquitin-protein ligase XBOS32 [Vitis vinifera][more]
gi|590580142|ref|XP_007013987.1|3.1e-18770.71XB3 in isoform 1 [Theobroma cacao][more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0008270zinc ion binding
GO:0005515protein binding
Vocabulary: INTERPRO
TermDefinition
IPR020683Ankyrin_rpt-contain_dom
IPR017907Znf_RING_CS
IPR013083Znf_RING/FYVE/PHD
IPR002110Ankyrin_rpt
IPR001841Znf_RING
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010311 lateral root formation
biological_process GO:0009693 ethylene biosynthetic process
biological_process GO:0008150 biological_process
biological_process GO:0042967 acyl-carrier-protein biosynthetic process
biological_process GO:0044699 single-organism process
biological_process GO:1901576 organic substance biosynthetic process
biological_process GO:0044267 cellular protein metabolic process
biological_process GO:0044281 small molecule metabolic process
biological_process GO:0010366 negative regulation of ethylene biosynthetic process
biological_process GO:0016567 protein ubiquitination
biological_process GO:0065007 biological regulation
biological_process GO:0016132 brassinosteroid biosynthetic process
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
biological_process GO:0051865 protein autoubiquitination
cellular_component GO:0005575 cellular_component
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0004842 ubiquitin-protein transferase activity
molecular_function GO:0005515 protein binding
molecular_function GO:0046872 metal ion binding
molecular_function GO:0008080 N-acetyltransferase activity
molecular_function GO:0003824 catalytic activity
molecular_function GO:0016874 ligase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
LsiUNG001000.1LsiUNG001000.1mRNA


Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 320..369
score: 0.
IPR001841Zinc finger, RING-typePROFILEPS50089ZF_RING_2coord: 320..370
score: 9
IPR002110Ankyrin repeatPRINTSPR01415ANKYRINcoord: 118..133
score: 3.1E-5coord: 196..210
score: 3.
IPR002110Ankyrin repeatSMARTSM00248ANK_2acoord: 50..79
score: 1.7E-5coord: 223..252
score: 9.7E-5coord: 117..147
score: 0.21coord: 180..209
score: 1.6E-4coord: 83..112
score: 0
IPR002110Ankyrin repeatPROFILEPS50088ANK_REPEATcoord: 50..82
score: 13.117coord: 223..255
score: 11.942coord: 117..138
score: 9.965coord: 83..115
score: 10.339coord: 180..212
score: 12
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3DG3DSA:3.30.40.10coord: 317..373
score: 1.
IPR017907Zinc finger, RING-type, conserved sitePROSITEPS00518ZF_RING_1coord: 335..344
scor
IPR020683Ankyrin repeat-containing domainGENE3DG3DSA:1.25.40.20coord: 19..278
score: 6.1
IPR020683Ankyrin repeat-containing domainPFAMPF12796Ank_2coord: 20..81
score: 1.4E-6coord: 88..207
score: 2.0
IPR020683Ankyrin repeat-containing domainPROFILEPS50297ANK_REP_REGIONcoord: 20..264
score: 4
IPR020683Ankyrin repeat-containing domainunknownSSF48403Ankyrin repeatcoord: 20..277
score: 1.44
NoneNo IPR availablePANTHERPTHR24128FAMILY NOT NAMEDcoord: 1..422
score: 2.4E
NoneNo IPR availablePANTHERPTHR24128:SF22SUBFAMILY NOT NAMEDcoord: 1..422
score: 2.4E
NoneNo IPR availablePFAMPF13857Ank_5coord: 223..263
score: 4.
NoneNo IPR availableunknownSSF57850RING/U-boxcoord: 319..376
score: 1.