Lsi11G014050 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi11G014050
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	O-methyltransferase
Location	chr11 : 22600673 .. 22606872 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATGATTCCTATGGAATTTAGCATAAATGGGAGTAATCCAAATCACATTTGGACGCTACACAAAGTGTTTGGAAAATTTCTAGTTTTGGTATGAGTTTGAATTTGGAGGGTGGAAAGATGTTAGAATGGGAGGAGACGAGTTGTTGGAAGTTCAATCCCACATATGGAATCACATCTTTAACTTCATAAACTCAATGTCTCTCAAATGTGCCATTCAACTTGGGATTCCAGACGCCATCCACAGTCATGGCCCCAACCCTATGCCCCTCTCTCTTCTTGTTTCATCCTAATAAAATTCAATTTATATATCGTTTGATGCGTTTATTGACTCATTATGGCTTCTTTGTTCTACAAGAAGAAGGATATATGTTGACCAATTCATCTCGCCTTCTTCTTAAAGACAACCCTTGCTCTATATCTCCCTTTCTACTTTCCATGCTTGAACCAGCTCTCTTAGAGCCATGACAATTTCTCTCAATTTGGTTCCAAACTGATGACCGAACGCCATTTGAGACCACTCATGGAAAGCCGTTTTGGGAGTACTTGGGGAATAAGCCGAAAGATGGGGATATTTTTAACGCAGGCATGGCAAGCGATGCGAGGTTGGTGATGAGAGTGTTATTGGAGAAACATAGAAGTGCTTTTGAGGGAGTTCGGTCGTTGGTTGATGTTGGTGGTGGTACAGGAACTATGGCCAAAGCTATTGCAAAGACTTTTCCAAAAATTGAATGCACTGTGCTTGATCTTCATCATTTAGGGTTTAAGGTAAAACCTACTTTCTATTTTTTTTTATGTCTCCATTTGATAACATCTTTTCATTTCTTTGTTTCTTGCTACTCGTTTTTTTATTTTCTATTTCTTATTTATTAAGAACTAAAAATCTAATCGTGTTTGATAACTATTTTTTTTGTTTTCTTAAAAAAAAATACATATATAAAAAACATAAATATGTTTGATAATTAATTATTTTTCTTAGTTGAAATAAAATTTAATAGTTAAAATTTTCTTTAAAAAAAGTGTAGTATGATAAAATATTTCATTAAAACAAATATAAAAACATTATAAATAATTTATAGGATTTAGTAGTAAAATTTCAGCTTCAAAGAAAATTGTAAAATAATAATAATATATTTTCATCTTAAATATTATTGGTTTACCTGAGTTAAATCAGTTAGACTAATTTGTAAATTGTTTGACAAATTTTGTAAAACTTTAAGAATCACTATTTTATCGCCTGAAAAAAACACTAATTTATGATAAAAAATATTGTTTTAGTTTTTATACTGTGAAGTTTATTCAATCTTAACCTCTATACTTTTAATTATCTAATTTAATCCTCATACTTTCAATCAATCTTAAATATAGTCTCATTGCTAGTTTATTGTTGATTTTGAAAAAAAATTGTTATATATTAATAATGTTTCTTACTGCGAAATCATACTCACATATTGTATTTCCTTACCTAATAATAATAATAGTTTGATCAATTTCGATAAAAAAAAATAAATTTTAAGGGACTAAATTTAAGATTTATTAAAAATACATGGACCAAAATTGGATAATTGAAAGTACAAAAACTAATTTGAACAAACTTTAAAGTATATAAATTAAAATGATATTTTTGTCTTAATTTATTGAACAACTTATTAATATATGTTTTTGTGTGCCAACTTCGCAATTAGACATTTATTGAAGATTGAACAATCAAGGATACTCATTATTATTATAAATATTTTCTTTTTTGCAAAAATATTGGATTACAATAACACAAAGTAGACATCACAATCTAAAAGCAAACACAATCAAAAGAATTAAGAAAAGAAAAAAAAAATAACAAACCAAATACATAGTACAAATGCATTCAACTCAAGGAAAGAGGGTCCAAAGGAGTTGAAAAAAAAAACCATTCATGAAAAAAGAAAAAAGAAGCATTAGTTGAAATTTAAAAAGGGGAAAGAATAAATATATATAAAAAAAAAAGAGAAATGGAAAAGAAAATGAGAGAAAAAAAAATGAAAAAGGAAAACGAATTGAAGGAGAGAAAGGAGGGAGAAAAATGAGAGATGGGTGTAGAAACTAGAAAGGGTGGGAGAAAATAGAAATATATATATATATATATATATATATATATTATATGTATAGAAAAATTAACTCGGATGGAAAAAATGTCAAACCATTTACAGAAAATAGAAAAAAAAATATTGATAGACATTAATAAACTTCTATTATTTCTATCACTGAAGTCTATCATTAATAGACTTCTATGAGCGTCTATCACGACTATTTAAAAATTTTGCTATTTTGTATAAATAGTTTCCTTTAATTTTTTACTTAGGTAAGAAATTTTATTAAACCTTTACTTAGCTCACAAAAAGAAAATTGCTCTATTTTTTTAAATAGTTTCAAGAGTGGTCACATTAATTGTTCTTTATATCCTACATAAAAATACCTAAATGAATTTAGAAAAGGCTTAAAGAAAAGAAAAGTTCAAGAGATCACAAAAAGTTGCACATATAGCCTAGAAAAATTCTCCCATAAACAACTCTTCTTACAGGATTTTTGCTATGTGCTTATAATAAAATCTAATCTTTGTCTATAATATGATATTTGGTGGTGACAAAATAGTGGATATTACATGACTTGGAGTGATGAAGAATGTGTGAAAATTCTGAAGAACTGTAAAGAAGCAATCACAAACAATGGTAACAAAGGAAAAGTAATGGTAATTGATTTGGTGGTGTCTAATACGAAAAAGGAAGACTTAATTGAGACTCAACTATTCTACGACATGTTGATGATGATTGTAGCAAGTGGAAAAGAGAGAGAAGAAAAAGAATGGGCTCAATTGATTAAAGAGGCTGGATTTAGTGCCGACAAAATCTCTCCTATTTTAGGCTTGAAGTCTCTTATAGAGATTTATCCATAGAACATAGGTGAGTAGAATTTTAACATTTTTTTTTGTTTTTTGTGAATCAAGTATTTGTGCTGATCTTAAACAGGAGATCTATTTTGGGTGTAATTTAAGAAGTGTTTGAGATTAAACTGATTTGCTTATTAAAGTATTGTGTCGATTTGAAGTGTGGACTTTGTTCTCAAAATGTATTAGAAAATTATATTTAATTTATGTTGGGGTAATTTTGGACAGAAAGGTATAATTGACCGAAAACAAGAATGAAAGTAACAAAGTCGAGAAAATAATAAGGTTCGAGCAAAAAAAATAGAACCTTGGGCCTAAAATGGACCTAGGGAAGAGGGATGGTTTGGGTCAAAACCCAAATTAGCGTGCTTAGCCTCAGCCTCAGCCAAGGCCAAGACCGGGTGACCCTAGCAAAACAACTAATATGTTTCACGGGTGCCTTGGCCCTAAGCATCACCCTAAGACCAAAATGGGCCTAGGGGGAGATGCTCGGCCTCGACCGCAAGCCAAACATGGAACCAGACACGTGTTTAGACCCAGTAGTTATATAATTAACCTAAGGCGACTCCAAAAACCTTGGAGAACAACCTAGGTCAACTTTCTCTATAAATACATTATTGTAACTTCATATGAGGTAACTTAAGTTCTACGTACTTTCAAACACTCTAGCTTCAACACTCTTTCACTTTCTGACTTAAGCATCGGAGTTTGTGTGACAAGCCCCACATCGATGTGCATGTGTTTCTTTTACATACCACATTCTTCTCCCCTTCACACAAATTCACTGTTGGTGTCACGCGAAGGCCAGGAGTGTTTTCCCTGTCCAAAATTTGGCATCAATAGTTGGCGCCTTCTATGGAGATGAAAGTGTTTTATTAATAATCTATATATCGTGAAGCTATCACTAACATGTGCTCAACACATTCAACTGTTGCATACCCACTATATGTTTCCTTAATTTGGATCAATATACTTCCGCCAGTCATAGTTTAACGCATCTACATAATTGCTTTAAGCGTCGTTACGCTATAACAAAGCACCGCGACACTCTGGTAGCTTCAGACGTGCACAGAAAACAGTATACTACGATAATACATACTACCTCCATTTAGCATTTCACATTTATTATTTTATTTAATAGAGTCCACACGACATGCTTATTATTTTATTTAATAGAGTCCACACGACGCTTAAATATTTTTTTAGAAGATATTCTTCAAGGTTTTTTTAATGGCAAATAATGAATGAGAGGTAGTGTGAAATATTTTGGAGAAAAAAAAACTTTTTTGGTCTATAGGTTTTGGGTCTAGTTTTAATTTGGTCCCTAAGTTTTAAAATATTACATTGTTAGTCCCTAACTTTTGAGTTTGTTTTCAATTTAGTCTCTAGGTTTTAAAATCTTGAAGTTTGAGTTTTGTTTCAATTTGGTCTATAATTTTTAAGATTTATACTTTTAACCTCAATTTTCACTAAATACTGTATTCAATATCAATGTCTATTAATATATTTAAACAAATTAGAAAGAAATATAATTGGTTAAGTTATTTACTATTTTTCATCAATAACTAATTCCAAAATTTCACTTGATAATTATTTTAAATTAATTAATAAACGCCAAAAACTGAAAATGAGTATTTAGCGTAAAAATAGTAAAAGTTAAAAGTGTAAATCTTAGAATCGAGAGACCAAATTGAAACAATTGAAGGTATAATTATAATATTTTAAAATCTAGAGATTAAATTAAAACCAAATTTAAAACTTAGTGACTAAGAGTATAACATTTTAAAAATTTTGGACCAAATAGAAACTAAACCTACAAAATTAGAGAAAAAATATTTTTTTTTCCCAAAAAAAAGAATTTCATGCTTCCCAATAGCTAGAAAATTTCACACATAGATGTAGCCCAGGTGGGGACTATAGCAATAAATGTTGTGTCTACAGAACAAAACTTCAAAAAAAAAAAAAAAAAAAGTTGGGTGTGAGGGGCATCACCCATCCACTTGTATGAATAATAATATTGTGAGGCTCATTGTTTCTACATACATTAAATTTTGGGGGCCCTATACTTTCTTCCTTTTTTTAATTTTTTTTTCTTTGGTGATAAGGCCTATGCATTTGTTTATAAATGAGAGGAACCAAATCACATTTGGAGTACACTACACAAAGTGTTTTGACAATTATTTCTATTTTTGGTATGAGTATGAATTTGAAGGGTAGAAAGATGTTAGCAATGGGAGGAGATGAGATGTTGGAAGCTCAATCCCACATATGGAATCACATCTTTAACTTCATAAACTCAATGTCTCTCAAATGTGTCATTCAACTTGGAATTCCAGATGTCATCCACAATCATGACCCTAACCCTATGCCCCTCTCTCTTCTTGTTTCATCCCTCAAACTTCATCCTAATAAAACCACATTCATTTACCGTTTAATGCGTTTATTGACTCACTCTGGTTTCTTTGTTCTACAAGAAGGATATATGTTGACCAATTCATCTCGTCTTCTTCTTAAAGACAATCATTGCGCTGTATCTCCTTTCGTACTTTCCATGCTTGAACCAGTTCTCTTAGAGCCATGGCAATTTTTCTCAATTTGGTTCCAAACTGACGATCGAACGCCATTTGAGACCACTCATGGAAAGCCGTTTTGGGAGTACTTGGGGAATAAGCCGAAAGATGGGGAGGCATGGCGAGCGATGCAAGGTTGGTGATAAGAGTGTTATTGGAGAAACATAGAGGTGCTTTTGAGGGAATTCGGTCGTTGGTTGATGTTGGTGGTGGGACAGGAACTATGGCCAAAGCCATTGCAAAGGATTTTCCAAAAATTGAATGCATTGTGCTTGATCTTCCTCAAGTTGTGGCTGACTTGAAAGCAGATCAACCAAACTTCAAATATGTTGAAGGGAACATGTTTAATGTTATTCCTCCTGCTGATGCTCTTTCCTTGAAGGTAAAACCTACTTTCTATTTTTTTTTGTTATGTCTCCATTTGATAGCATCTTTTCGTTTTTTATTTCTCGCCACTCGTTTTTTATTTTCTATTTCTTGTTTAAGAACTAAAAATCTAATCGTGTTTGTATTTTTTTCTTAAAAAAGAATATAATAAAAAAAAATGTAAATATGTTTGATAATAATTTCCTATTTTCTTAGTTGAAAATACGAAAAAGAATGGAAAGAGAAAAAAAAGAATGGGGTCAATTGATTAAAGAAGCTGGATTTGGTGCCTACAAAATTTTCTCCATTTTAGGCTTGAGGCCTCTTATAGAGATTTACCCATGA

mRNA sequence

ATGATGATTCCTATGGAATTTAGCATAAATGGGAGTAATCCAAATCACATTTGGACGCTACACAAAGTGTTTGGAAAATTTCTAGTTTTGGGTAGAAAGATGTTAGCAATGGGAGGAGATGAGATGTTGGAAGCTCAATCCCACATATGGAATCACATCTTTAACTTCATAAACTCAATGTCTCTCAAATGTGTCATTCAACTTGGAATTCCAGATGTCATCCACAATCATGACCCTAACCCTATGCCCCTCTCTCTTCTTGTTTCATCCCTCAAACTTCATCCTAATAAAACCACATTCATTTACCGTTTAATGCGTTTATTGACTCACTCTGGTTTCTTTGTTCTACAAGAAGGATATATGTTGACCAATTCATCTCGTCTTCTTCTTAAAGACAATCATTGCGCTGTATCTCCTTTCGTACTTTCCATGCTTGAACCAGTTCTCTTAGAGCCATGGCAATTTTTCTCAATTTGGTTCCAAACTGACGATCGAACGCCATTTGAGACCACTCATGGAAAGCCGTTTTGGGAGTACTTGGGGAATAAGCCGAAAGATGGGGAGGCATGGCGAGCGATGCAAGGAACTATGGCCAAAGCCATTGCAAAGGATTTTCCAAAAATTGAATGCATTGTGCTTGATCTTCCTCAAGTTGTGGCTGACTTGAAAGCAGATCAACCAAACTTCAAATATGTTGAAGGGAACATGTTTAATGTTATTCCTCCTGCTGATGCTCTTTCCTTGAAGTTGAAAATACGAAAAAGAATGGAAAGAGAAAAAAAAGAATGGGGTCAATTGATTAAAGAAGCTGGATTTGGTGCCTACAAAATTTTCTCCATTTTAGGCTTGAGGCCTCTTATAGAGATTTACCCATGA

Coding sequence (CDS)

ATGATGATTCCTATGGAATTTAGCATAAATGGGAGTAATCCAAATCACATTTGGACGCTACACAAAGTGTTTGGAAAATTTCTAGTTTTGGGTAGAAAGATGTTAGCAATGGGAGGAGATGAGATGTTGGAAGCTCAATCCCACATATGGAATCACATCTTTAACTTCATAAACTCAATGTCTCTCAAATGTGTCATTCAACTTGGAATTCCAGATGTCATCCACAATCATGACCCTAACCCTATGCCCCTCTCTCTTCTTGTTTCATCCCTCAAACTTCATCCTAATAAAACCACATTCATTTACCGTTTAATGCGTTTATTGACTCACTCTGGTTTCTTTGTTCTACAAGAAGGATATATGTTGACCAATTCATCTCGTCTTCTTCTTAAAGACAATCATTGCGCTGTATCTCCTTTCGTACTTTCCATGCTTGAACCAGTTCTCTTAGAGCCATGGCAATTTTTCTCAATTTGGTTCCAAACTGACGATCGAACGCCATTTGAGACCACTCATGGAAAGCCGTTTTGGGAGTACTTGGGGAATAAGCCGAAAGATGGGGAGGCATGGCGAGCGATGCAAGGAACTATGGCCAAAGCCATTGCAAAGGATTTTCCAAAAATTGAATGCATTGTGCTTGATCTTCCTCAAGTTGTGGCTGACTTGAAAGCAGATCAACCAAACTTCAAATATGTTGAAGGGAACATGTTTAATGTTATTCCTCCTGCTGATGCTCTTTCCTTGAAGTTGAAAATACGAAAAAGAATGGAAAGAGAAAAAAAAGAATGGGGTCAATTGATTAAAGAAGCTGGATTTGGTGCCTACAAAATTTTCTCCATTTTAGGCTTGAGGCCTCTTATAGAGATTTACCCATGA

Protein sequence

MMIPMEFSINGSNPNHIWTLHKVFGKFLVLGRKMLAMGGDEMLEAQSHIWNHIFNFINSMSLKCVIQLGIPDVIHNHDPNPMPLSLLVSSLKLHPNKTTFIYRLMRLLTHSGFFVLQEGYMLTNSSRLLLKDNHCAVSPFVLSMLEPVLLEPWQFFSIWFQTDDRTPFETTHGKPFWEYLGNKPKDGEAWRAMQGTMAKAIAKDFPKIECIVLDLPQVVADLKADQPNFKYVEGNMFNVIPPADALSLKLKIRKRMEREKKEWGQLIKEAGFGAYKIFSILGLRPLIEIYP

Homology

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BLAST of Lsi11G014050 vs. Swiss-Prot
Match: ROMT_VITVI (Trans-resveratrol di-O-methyltransferase OS=Vitis vinifera GN=ROMT PE=1 SV=2)

HSP 1 Score: 223.8 bits (569), Expect = 2.5e-57
Identity = 139/349 (39.83%), Postives = 177/349 (50.72%), Query Frame = 1


Query: 41  EMLEAQSHIWNHIFNFINSMSLKCVIQLGIPDVIHNHDPNPMPLSLLVSSLKLHPNKTTF 100
           E+L AQ+H+WNHIFNFI SMSLKC IQLGIPD+IHNH   PM L  LV+ L +HP ++  
Sbjct: 11  ELLHAQAHVWNHIFNFIKSMSLKCAIQLGIPDIIHNHG-KPMTLPELVAKLPVHPKRSQC 70

Query: 101 IYRLMRLLTHSGFFVLQ--------EGYMLTNSSRLLLKDNHCAVSPFVLSMLEPVLLEP 160
           +YRLMR+L HSGF   Q        EGY+LT++SRLLL D+  ++ P VL+ML+P+L +P
Sbjct: 71  VYRLMRILVHSGFLAAQRVQQGKEEEGYVLTDASRLLLMDDSLSIRPLVLAMLDPILTKP 130

Query: 161 WQFFSIWFQTDDRTPFETTH--------------GKPFWEYLGNKP--------KDGEAW 220
           W + S WFQ DD TPF T +                 F E + +          K+G+  
Sbjct: 131 WHYLSAWFQNDDPTPFHTAYERSFWDYAGHEPQLNNSFNEAMASDARLLTSVLLKEGQGV 190

Query: 221 RAM----------QGTMAKAIAKDFPKIECIVLDLPQVVADLKADQPNFKYVEGNMFNVI 280
            A            G +AKAIA  FP + C VLDL  VVA L+  + N  Y  G+MF  I
Sbjct: 191 FAGLNSLVDVGGGTGKVAKAIANAFPHLNCTVLDLSHVVAGLQGSK-NLNYFAGDMFEAI 250

Query: 281 PPADALSLK-----------LKIRKRM--------------------------------- 292
           PPADA+ LK           +KI KR                                  
Sbjct: 251 PPADAILLKWILHDWSNEECVKILKRCREAIPSKENGGKVIIIDMIMMKNQGDYKSTETQ 310

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BLAST of Lsi11G014050 vs. Swiss-Prot
Match: CVMT1_OCIBA (Chavicol O-methyltransferase OS=Ocimum basilicum GN=CVOMT1 PE=1 SV=1)

HSP 1 Score: 174.9 bits (442), Expect = 1.3e-42
Identity = 117/351 (33.33%), Postives = 165/351 (47.01%), Query Frame = 1


Query: 35  LAMGGDEMLEAQSHIWNHIFNFINSMSLKCVIQLGIPDVIHNHDPNPMPLSLLVSSLKLH 94
           +++  +++L+AQ+H+WNH++ F NSMSLKC IQLGIPD++H HD +PM LS L+ ++ ++
Sbjct: 8   ISLSTEQLLQAQAHVWNHMYAFANSMSLKCAIQLGIPDILHKHD-HPMTLSQLLKAIPIN 67

Query: 95  PNKTTFIYRLMRLLTHSGFFVLQEG------YMLTNSSRLLLKDNHCAVSPFVLSMLEPV 154
             K+    RLMR L +S FF+ +        Y LT +SRLLLK     V+P V  +L+P 
Sbjct: 68  KEKSQSFQRLMRALVNSNFFIEENSNNQEVCYWLTPASRLLLKGAPLTVAPLVQVVLDPT 127

Query: 155 LLEPWQFFSIWFQTDDR-TPFETTHGKPFWEYL------GNKPKDGEAWRAM-------- 214
              PW + S WF+ ++  T FE  +G  FWE L      G    +  +  +         
Sbjct: 128 FTNPWHYMSEWFKHENHATQFEAANGCTFWEKLANKPSMGRFFDEAMSCDSRLVAHVLTK 187

Query: 215 ------------------QGTMAKAIAKDFPKIECIVLDLPQVVADLKADQPNFKYVEGN 274
                              GTMAKAI +  P ++C VLDLP VVA L++      Y+ G+
Sbjct: 188 DYKHVIDGIRTLVDVGGGNGTMAKAIVEAVPTMKCTVLDLPHVVAGLESTD-KLSYIGGD 247

Query: 275 MFNVIPPADALSLK-----------LKIRKRMEREK------------------------ 292
           MF  IP ADA+ LK           LKI KR +                           
Sbjct: 248 MFQSIPSADAILLKFIIHDWDDEEGLKILKRCKDAVGIGGKVIIIDVVVGVNHDVDEVLE 307

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BLAST of Lsi11G014050 vs. Swiss-Prot
Match: Q8OMT_MENPI (8-hydroxyquercetin 8-O-methyltransferase OS=Mentha piperita GN=OMT2 PE=1 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 2.5e-41
Identity = 109/277 (39.35%), Postives = 145/277 (52.35%), Query Frame = 1


Query: 41  EMLEAQSHIWNHIFNFINSMSLKCVIQLGIPDVIHNHDPNPMPLSLLVSSLKLHPNKTTF 100
           E+LEAQ+H+WNHI+++INSMSLKC IQLGIPD IH H  NP+ LS L  +L ++  K+  
Sbjct: 12  ELLEAQAHVWNHIYSYINSMSLKCAIQLGIPDAIHKHG-NPITLSQLADALNINKAKSHG 71

Query: 101 IYRLMRLLTHSGFF------VLQEG---------YMLTNSSRLLLKDNHCAVSPFVLSML 160
           ++RLMR+L HSGFF      V  EG         Y LT +SRLLL+    +V+PF L+M 
Sbjct: 72  LFRLMRILVHSGFFDKVKVKVKVEGEDEEEEEDAYSLTPASRLLLRSEPLSVAPFALAMS 131

Query: 161 EPVLLEPWQFFSIWFQTDDRTPFETTHGKPF---------WEYLGNKPKDGEAWRAMQ-- 220
           +PV  E W   S WF+ D    F+T +G  F            L N+    +A       
Sbjct: 132 DPVYTETWHHLSEWFRNDAVAAFDTKYGMTFPEYAVADDRLNVLFNEAMACDAGFVNSIL 191

Query: 221 ---------------------GTMAKAIAKDFPKIECIVLDLPQVVADLKADQPNFKYVE 271
                                G  AK IA  FP +EC VLDLP VV  LK  + N  +V 
Sbjct: 192 TTECREIFDGLESMVDVGGGTGATAKGIAAAFPGMECTVLDLPNVVGGLKGSE-NLSFVS 251

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BLAST of Lsi11G014050 vs. Swiss-Prot
Match: MOMT_CATRO (Myricetin O-methyltransferase OS=Catharanthus roseus PE=1 SV=1)

HSP 1 Score: 166.8 bits (421), Expect = 3.6e-40
Identity = 103/281 (36.65%), Postives = 154/281 (54.80%), Query Frame = 1


Query: 35  LAMGGDEMLEAQSHIWNHIFNFINSMSLKCVIQLGIPDVIHNHDPNPMPLSLLVSSLKLH 94
           + +   E+  AQ+H +  +F+F +  SLKC +QLGIPD IH+H   PM LS L +SL ++
Sbjct: 1   MELQSSEIRNAQAHFFTQVFSFTSMSSLKCAVQLGIPDAIHSHG-KPMALSDLTNSLPIN 60

Query: 95  PNKTTFIYRLMRLLTHSGFFVLQEG--YMLTNSSRLLLKDNHCAVSPFVLSMLEPVLLEP 154
           P+K  +IYRLMR+L  +G+F  +E   Y LT  +RLLLK++       VL + +   L+ 
Sbjct: 61  PSKAPYIYRLMRILVAAGYFSEEEKNVYSLTPFTRLLLKNDPLNSISMVLGVNQIAELKA 120

Query: 155 WQFFSIWFQTDDRTPFETTHGKPFWEYLGNKPKDGEAWRAMQ------------------ 214
           W   S WFQ +D T FET HGK FW++ G + K G+ +  +                   
Sbjct: 121 WNAMSEWFQNEDLTAFETAHGKNFWDF-GAEDKYGKNFDGVMAADSILVSKMLIPEFNYL 180

Query: 215 --------------GTMAKAIAKDFPKIECIVLDLPQVVADLKADQPNFKYVEGNMFNVI 268
                         GT+AKAIAK FP ++C V DLP VVA+L++ + N ++V G+MF  I
Sbjct: 181 FEGLDSLVDVGGGTGTIAKAIAKSFPDLKCTVFDLPHVVANLESTE-NLEFVGGDMFEKI 240

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BLAST of Lsi11G014050 vs. Swiss-Prot
Match: 7OMT9_MEDSA (Isoflavone-7-O-methyltransferase 9 OS=Medicago sativa PE=2 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 2.7e-35
Identity = 112/343 (32.65%), Postives = 157/343 (45.77%), Query Frame = 1


Query: 41  EMLEAQSHIWNHIFNFINSMSLKCVIQLGIPDVIHNHDPNPMPLSLLVSSLKLHPNKTTF 100
           E+ +AQ+ ++ HI+ FI+SMSLK  + + IP++IHNH   P+ LS LVS L++  +K   
Sbjct: 12  EIFKAQALLYKHIYAFIDSMSLKWAVGMNIPNIIHNHG-KPISLSNLVSILQVPSSKIGN 71

Query: 101 IYRLMRLLTHSGFFVL----QEGYMLTNSSRLLLKDNHCAVSPFVLSMLEPVLLEPWQFF 160
           + RLMR L H+GFF +    +E Y LT +S LL++ +   ++P V  +L+P L   +   
Sbjct: 72  VRRLMRYLAHNGFFEIITKEEESYALTVASELLVRGSDLCLAPMVECVLDPTLSGSYHEL 131

Query: 161 SIWFQTDDRTPFETTHGKPFWEYLGNKPKDGEAWR-AM---------------------- 220
             W   +D T F  T G  FW++L   P+   ++  AM                      
Sbjct: 132 KKWIYEEDLTLFGVTLGSGFWDFLDKNPEYNTSFNDAMASDSKLINLALRDCDFVFDGLE 191

Query: 221 --------QGTMAKAIAKDFPKIECIVLDLPQVVADLKADQPNFKYVEGNMFNVIPPADA 280
                    GT AK I + FPK++CIV D PQVV +L     N  YV G+MF  IP ADA
Sbjct: 192 SIVDVGGGTGTTAKIICETFPKLKCIVFDRPQVVENLSGSN-NLTYVGGDMFTSIPNADA 251

Query: 281 LSLK-----------LKIRKRME-------------------REKK-------------- 292
           + LK           L+I K+ +                    EKK              
Sbjct: 252 VLLKYILHNWTDKDCLRILKKCKEAVTNDGKRGKVTIIDMVINEKKDENQVTQIKLLMDV 311

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BLAST of Lsi11G014050 vs. TrEMBL
Match: A0A0A0K1E9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G039280 PE=3 SV=1)

HSP 1 Score: 337.4 bits (864), Expect = 1.7e-89
Identity = 186/351 (52.99%), Postives = 216/351 (61.54%), Query Frame = 1


Query: 31  GRKMLAMGGDEMLEAQSHIWNHIFNFINSMSLKCVIQLGIPDVIHNHDPNPMPLSLLVSS 90
           G  MLAMGGDE+LEAQSHIWNHIFNFINSMSLKC IQLGIPD IH+H PNP+PLS+LVSS
Sbjct: 30  GGNMLAMGGDELLEAQSHIWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPVPLSILVSS 89

Query: 91  LKLHPNKTTFIYRLMRLLTHSGFFVLQ-EGYMLTNSSRLLLKDNHCAVSPFVLSMLEPVL 150
           L+LHPNKT FIYRLMRLLTHSGFFV Q EGY+LTNSSRLLLKDN CAVSPF+LSML+P L
Sbjct: 90  LQLHPNKTQFIYRLMRLLTHSGFFVQQEEGYILTNSSRLLLKDNPCAVSPFLLSMLQPAL 149

Query: 151 LEPWQFFSIWFQTDDRTPFETTHGKPFWEYLGNKPKDGEAWRAMQGTMAKAI-------- 210
            +PWQF SIWFQTDD+TPFET HG PFW+Y+ NKPK+GE + A   + A+ +        
Sbjct: 150 TDPWQFLSIWFQTDDQTPFETAHGVPFWKYMRNKPKEGEVFNAGMASDARLVNSVLLGKH 209

Query: 211 ------AKDFPKI--------ECIVLDLP----------QVVADLKADQPNFKYVEGNMF 270
                  K    +        + I    P          QVVA L+ DQPN  YVEG+MF
Sbjct: 210 KSVFEGVKSLVDVGGGTGTMAKAISQAFPQMECTVFDLPQVVAHLRGDQPNLNYVEGDMF 269

Query: 271 NVIPPADALSLK-----------LKIRKRME----------------------------- 292
             IPPAD L LK           ++I K  +                             
Sbjct: 270 KRIPPADVLLLKWILHDWSDEECVEILKNCKAAIRSNGNKGKVMVIDIVLFGNYKKDSME 329

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BLAST of Lsi11G014050 vs. TrEMBL
Match: A0A0A0K5D5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G039790 PE=3 SV=1)

HSP 1 Score: 331.6 bits (849), Expect = 9.3e-88
Identity = 171/277 (61.73%), Postives = 198/277 (71.48%), Query Frame = 1


Query: 31  GRKMLAMGGDEMLEAQSHIWNHIFNFINSMSLKCVIQLGIPDVIHNHDPNPMPLSLLVSS 90
           G KMLAMGGDE+LEAQSH+WNHIFNFINSMSLKC IQLGIPD IH+H PNP+PLS+LVSS
Sbjct: 5   GGKMLAMGGDELLEAQSHVWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPVPLSILVSS 64

Query: 91  LKLHPNKTTFIYRLMRLLTHSGFFVLQ-EGYMLTNSSRLLLKDNHCAVSPFVLSMLEPVL 150
           L+LHPNKT FIYRLMRLLTHSGFFV Q EGY+LTNSSRLLLKDN CAVSPF+LSML+P L
Sbjct: 65  LQLHPNKTQFIYRLMRLLTHSGFFVQQEEGYILTNSSRLLLKDNPCAVSPFLLSMLQPAL 124

Query: 151 LEPWQFFSIWFQTDDRTPFETTHGKPFWEYLGNKPKDGEAWRAMQGTMAKAI-------- 210
            +PWQF SIWFQTDD+TPFET HG PFW+Y+ NKPK+GE + A   + A+ +        
Sbjct: 125 TDPWQFLSIWFQTDDQTPFETAHGVPFWKYMRNKPKEGEVFNAGMASDARLVINVLLEKH 184

Query: 211 AKDFPKIECIV------------------------LDLPQVVADLKADQPNFKYVEGNMF 270
              F  +E +V                        LDLPQVVA LK DQPNFKYVEG+MF
Sbjct: 185 RSVFEGVESLVDVGGGTGTVAKAISQAFPQMECTVLDLPQVVAHLKGDQPNFKYVEGDMF 244

Query: 271 NVIPPADALSLKLKIRKRMEREKKEWGQLIKEAGFGA 275
            +IPPAD + LK  +    + E  E  +  KEA  G+
Sbjct: 245 TLIPPADTILLKWILHDWSDEECVEILKKCKEAITGS 281

[Close]

BLAST of Lsi11G014050 vs. TrEMBL
Match: A0A0A0K6Q4_CUCSA (O-methyltransferase OS=Cucumis sativus GN=Csa_7G039780 PE=3 SV=1)

HSP 1 Score: 292.0 bits (746), Expect = 8.2e-76
Identity = 150/253 (59.29%), Postives = 177/253 (69.96%), Query Frame = 1


Query: 31  GRKMLAMGGDEMLEAQSHIWNHIFNFINSMSLKCVIQLGIPDVIHNHDPNPMPLSLLVSS 90
           G  M+ M GDE++EA   I  HIFNFINSMSLKC IQLGIPD IH+H P+P+PLS LVSS
Sbjct: 5   GGNMVEMEGDELMEAHGQICKHIFNFINSMSLKCAIQLGIPDAIHSHGPSPLPLSRLVSS 64

Query: 91  LKLHPNKTTFIYRLMRLLTHSGFFVLQ-EGYMLTNSSRLLLKDNHCAVSPFVLSMLEPVL 150
           L+LHPNKT FIYRLMRLLTHSGFFVLQ EGY+LT SS LLLKDN C +SPF+LSML+PV 
Sbjct: 65  LQLHPNKTQFIYRLMRLLTHSGFFVLQEEGYVLTTSSHLLLKDNSCTLSPFLLSMLQPVF 124

Query: 151 LEPWQFFSIWFQTDDRTPFETTHGKPFWEYLGNKPKDGEAWRAMQGTMAKAI-------- 210
           +EP  F S WF+TDD+TPFET HGK FWE++GNK KDG+ + A   + A+ +        
Sbjct: 125 VEPSHFLSAWFRTDDQTPFETAHGKSFWEFVGNKRKDGDTFNAGMASDARLVMSVLMGKH 184

Query: 211 AKDFPKIECIV------------------------LDLPQVVADLKADQPNFKYVEGNMF 251
              F  +E +V                        LDLPQVVA+LK D PNFKYVEG+MF
Sbjct: 185 TSVFEGVESLVDVGGGTGTMAKAIAKAFPQIECTVLDLPQVVAELKPDIPNFKYVEGDMF 244

[Close]

BLAST of Lsi11G014050 vs. TrEMBL
Match: F6H5H5_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_12s0028g02850 PE=3 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 8.5e-65
Identity = 149/357 (41.74%), Postives = 190/357 (53.22%), Query Frame = 1


Query: 35  LAMG--GDEMLEAQSHIWNHIFNFINSMSLKCVIQLGIPDVIHNHDPNPMPLSLLVSSLK 94
           LA+G    E+L A +H+WNHIFNFINSMSLKC I+LGIPD+IHNH   PM LS LV+ L 
Sbjct: 3   LAVGETSTELLHAHAHVWNHIFNFINSMSLKCAIELGIPDIIHNHG-KPMTLSELVAELP 62

Query: 95  LHPNKTTFIYRLMRLLTHSGFFVL--------QEGYMLTNSSRLLLKDNHCAVSPFVLSM 154
           ++P KT  +YRLMRLL  SGFF          +EGY+LT++SRLLLKD+  +  PF+L+M
Sbjct: 63  VNPEKTKCVYRLMRLLVQSGFFTRKRVQESGQEEGYVLTHASRLLLKDDPLSARPFLLAM 122

Query: 155 LEPVLLEPWQFFSIWFQTDDRTPFETTHGKPFWEYLGNKPKDGEAWRAMQ---------- 214
           L+PVL+ PWQ+ S WFQ DD TPF+T HG+ FW+Y G++PK    +              
Sbjct: 123 LDPVLITPWQYVSAWFQNDDPTPFDTAHGRTFWDYAGHEPKLNNFFNEAMASDARLVTSV 182

Query: 215 ----------------------GTMAKAIAKDFPKIECIVLDLPQVVADLKADQPNFKYV 274
                                 GT+A+AIA  FP + C VLDLP VVA L+  + N  Y+
Sbjct: 183 LIKDCKGIFVGLNSLVDVGGGTGTVARAIANAFPHLNCTVLDLPHVVAGLEGSK-NLNYL 242

Query: 275 EGNMFNVIPPADALSLK-----------LKIRKR-------------------------- 292
            G+MF  IPPADA+ LK           +KI KR                          
Sbjct: 243 AGDMFEAIPPADAILLKWILHDWNHDECVKILKRCRDAIPSKEKGGKVIIIDMMMENQKA 302

[Close]

BLAST of Lsi11G014050 vs. TrEMBL
Match: F6H5F7_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_12s0028g02950 PE=3 SV=1)

HSP 1 Score: 252.3 bits (643), Expect = 7.2e-64
Identity = 147/349 (42.12%), Postives = 188/349 (53.87%), Query Frame = 1


Query: 41  EMLEAQSHIWNHIFNFINSMSLKCVIQLGIPDVIHNHDPNPMPLSLLVSSLKLHPNKTTF 100
           E+L+AQ+H+WNHIFNF+NSMSLKC IQLGIPD+IHNH   PM L  LV+ L +HP KT +
Sbjct: 11  ELLQAQAHVWNHIFNFVNSMSLKCAIQLGIPDIIHNHG-KPMTLPQLVAKLPVHPKKTQY 70

Query: 101 IYRLMRLLTHSGFFVL--------QEGYMLTNSSRLLLKDNHCAVSPFVLSMLEPVLLEP 160
           +YRLMR+L  S FF          +EGY+LT++SRLLLKD+  +V PF+L+ML+P L +P
Sbjct: 71  VYRLMRVLVQSDFFAAQRAQQSEEEEGYVLTHASRLLLKDDSLSVRPFLLAMLDPTLTKP 130

Query: 161 WQFFSIWFQTDDRTPFETTHGKPFWEYLGNKP----------------------KDG--- 220
           W + S WFQ DD TPF+T H +  W+Y G++P                      K+G   
Sbjct: 131 WHYVSAWFQNDDPTPFDTAHERTLWDYAGHEPQLNYFFNEAMASDARLVTSVLVKEGKGV 190

Query: 221 -EAWRAM------QGTMAKAIAKDFPKIECIVLDLPQVVADLKADQPNFKYVEGNMFNVI 280
            E   ++       GT+AKAIA  FP ++C VLDLP VVA L+  + N  Y  G+MF  I
Sbjct: 191 FEGLNSLVDVGGGTGTVAKAIANAFPHLKCTVLDLPHVVAGLQGSK-NLNYFAGDMFEAI 250

Query: 281 PPADALSLK-----------LKIRKRMER------------------------------- 292
           PPADA+ LK           +KI KR                                  
Sbjct: 251 PPADAILLKWILHDWSDEECVKILKRCREAIPSKERGGKVIIIDMMVKNQKGDCKSRETQ 310

[Close]

BLAST of Lsi11G014050 vs. TAIR10
Match: AT4G35160.1 (AT4G35160.1 O-methyltransferase family protein)

HSP 1 Score: 89.0 bits (219), Expect = 5.3e-18
Identity = 72/256 (28.12%), Postives = 111/256 (43.36%), Query Frame = 1


Query: 40  DEMLEAQSHIWNHIFNFINSMSLKCVIQLGIPDVIHNHDPN-PMPLSLLVSSLKLHPNKT 99
           DE  +A   IW ++F F +  + KC I L IP+ I NH  + P+ L+ L S++   P+  
Sbjct: 24  DEEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLAELSSAVSASPSH- 83

Query: 100 TFIYRLMRLLTHSGFFV-------LQEGYMLTNSSRLLL--KDNHCAVSPFVLSMLEPVL 159
             + R+MR L H G F        L  GY+ T  SR L+  + +  +++PFVL    P +
Sbjct: 84  --LRRIMRFLVHQGIFKEIPTKDGLATGYVNTPLSRRLMITRRDGKSLAPFVLFETTPEM 143

Query: 160 LEPWQFFSIWFQT----DDRTPFETTHGKPFWEYLGNKP------KDGEAWRAMQ----- 219
           L PW   S    +        PF+  HGK  W +  + P       +  A  A +     
Sbjct: 144 LAPWLRLSSVVSSPVNGSTPPPFDAVHGKDVWSFAQDNPFLSDMINEAMACDARRVVPRV 203

Query: 220 ---------------------GTMAKAIAKDFPKIECIVLDLPQVVADLKADQPNFKYVE 250
                                G     + K+FP I+    DLP V+ ++       + VE
Sbjct: 204 AGACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFNFDLPHVI-EVAEVLDGVENVE 263

[Close]

BLAST of Lsi11G014050 vs. TAIR10
Match: AT3G53140.1 (AT3G53140.1 O-methyltransferase family protein)

HSP 1 Score: 60.1 bits (144), Expect = 2.7e-09
Identity = 63/249 (25.30%), Postives = 103/249 (41.37%), Query Frame = 1


Query: 60  MSLKCVIQLGIPDVIHNHDPNPMPLSLLVSSLKLH-PNKTTF------IYRLMRLLTHSG 119
           MSL   ++LGI D I N   N  PLS      +LH P+ TT       + R++R+LT  G
Sbjct: 27  MSLNAAVRLGIADAIWNGGANS-PLSAAEILPRLHLPSHTTIGGDPENLQRILRMLTSYG 86

Query: 120 FFV------LQEGYMLTNSSRLLLKDNH-CAVSPFVLSMLEPVLLEPWQFFSIWFQTDDR 179
            F       ++  Y LT+  + L+ D+   + + +VL   +  L+  W          + 
Sbjct: 87  VFSEHLVGSIERKYSLTDVGKTLVTDSGGLSYAAYVLQHHQEALMRAWPLVHTAVVEPET 146

Query: 180 TPFETTHGKPFWEYLGNKPK-DGEAWRAMQGTMA-------------------------- 239
            P+   +G+  +   G   + +G   +AM G                             
Sbjct: 147 EPYVKANGEAAYAQYGKSEEMNGLMQKAMSGVSVPFMKAILDGYDGFKSVDILVDVGGSA 206

Query: 240 ----KAIAKDFPKI-ECIVLDLPQVVADLKADQPNFKYVEGNMFNVIPPADALSLKLKIR 263
               + I + FP + E I  DLP+VVA    + P   +V G+MF  +P ADA+ +K  + 
Sbjct: 207 GDCLRMILQQFPNVREGINFDLPEVVAKAP-NIPGVTHVGGDMFQSVPSADAIFMKWVLT 266

[Close]

BLAST of Lsi11G014050 vs. NCBI nr
Match: gi|778723655|ref|XP_004151089.2| (PREDICTED: trans-resveratrol di-O-methyltransferase-like [Cucumis sativus])

HSP 1 Score: 337.4 bits (864), Expect = 2.4e-89
Identity = 186/351 (52.99%), Postives = 216/351 (61.54%), Query Frame = 1


Query: 31  GRKMLAMGGDEMLEAQSHIWNHIFNFINSMSLKCVIQLGIPDVIHNHDPNPMPLSLLVSS 90
           G  MLAMGGDE+LEAQSHIWNHIFNFINSMSLKC IQLGIPD IH+H PNP+PLS+LVSS
Sbjct: 5   GGNMLAMGGDELLEAQSHIWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPVPLSILVSS 64

Query: 91  LKLHPNKTTFIYRLMRLLTHSGFFVLQ-EGYMLTNSSRLLLKDNHCAVSPFVLSMLEPVL 150
           L+LHPNKT FIYRLMRLLTHSGFFV Q EGY+LTNSSRLLLKDN CAVSPF+LSML+P L
Sbjct: 65  LQLHPNKTQFIYRLMRLLTHSGFFVQQEEGYILTNSSRLLLKDNPCAVSPFLLSMLQPAL 124

Query: 151 LEPWQFFSIWFQTDDRTPFETTHGKPFWEYLGNKPKDGEAWRAMQGTMAKAI-------- 210
            +PWQF SIWFQTDD+TPFET HG PFW+Y+ NKPK+GE + A   + A+ +        
Sbjct: 125 TDPWQFLSIWFQTDDQTPFETAHGVPFWKYMRNKPKEGEVFNAGMASDARLVNSVLLGKH 184

Query: 211 ------AKDFPKI--------ECIVLDLP----------QVVADLKADQPNFKYVEGNMF 270
                  K    +        + I    P          QVVA L+ DQPN  YVEG+MF
Sbjct: 185 KSVFEGVKSLVDVGGGTGTMAKAISQAFPQMECTVFDLPQVVAHLRGDQPNLNYVEGDMF 244

Query: 271 NVIPPADALSLK-----------LKIRKRME----------------------------- 292
             IPPAD L LK           ++I K  +                             
Sbjct: 245 KRIPPADVLLLKWILHDWSDEECVEILKNCKAAIRSNGNKGKVMVIDIVLFGNYKKDSME 304

[Close]

BLAST of Lsi11G014050 vs. NCBI nr
Match: gi|700188247|gb|KGN43480.1| (hypothetical protein Csa_7G039280 [Cucumis sativus])

HSP 1 Score: 337.4 bits (864), Expect = 2.4e-89
Identity = 186/351 (52.99%), Postives = 216/351 (61.54%), Query Frame = 1


Query: 31  GRKMLAMGGDEMLEAQSHIWNHIFNFINSMSLKCVIQLGIPDVIHNHDPNPMPLSLLVSS 90
           G  MLAMGGDE+LEAQSHIWNHIFNFINSMSLKC IQLGIPD IH+H PNP+PLS+LVSS
Sbjct: 30  GGNMLAMGGDELLEAQSHIWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPVPLSILVSS 89

Query: 91  LKLHPNKTTFIYRLMRLLTHSGFFVLQ-EGYMLTNSSRLLLKDNHCAVSPFVLSMLEPVL 150
           L+LHPNKT FIYRLMRLLTHSGFFV Q EGY+LTNSSRLLLKDN CAVSPF+LSML+P L
Sbjct: 90  LQLHPNKTQFIYRLMRLLTHSGFFVQQEEGYILTNSSRLLLKDNPCAVSPFLLSMLQPAL 149

Query: 151 LEPWQFFSIWFQTDDRTPFETTHGKPFWEYLGNKPKDGEAWRAMQGTMAKAI-------- 210
            +PWQF SIWFQTDD+TPFET HG PFW+Y+ NKPK+GE + A   + A+ +        
Sbjct: 150 TDPWQFLSIWFQTDDQTPFETAHGVPFWKYMRNKPKEGEVFNAGMASDARLVNSVLLGKH 209

Query: 211 ------AKDFPKI--------ECIVLDLP----------QVVADLKADQPNFKYVEGNMF 270
                  K    +        + I    P          QVVA L+ DQPN  YVEG+MF
Sbjct: 210 KSVFEGVKSLVDVGGGTGTMAKAISQAFPQMECTVFDLPQVVAHLRGDQPNLNYVEGDMF 269

Query: 271 NVIPPADALSLK-----------LKIRKRME----------------------------- 292
             IPPAD L LK           ++I K  +                             
Sbjct: 270 KRIPPADVLLLKWILHDWSDEECVEILKNCKAAIRSNGNKGKVMVIDIVLFGNYKKDSME 329

[Close]

BLAST of Lsi11G014050 vs. NCBI nr
Match: gi|659110928|ref|XP_008455485.1| (PREDICTED: trans-resveratrol di-O-methyltransferase-like [Cucumis melo])

HSP 1 Score: 337.4 bits (864), Expect = 2.4e-89
Identity = 173/252 (68.65%), Postives = 188/252 (74.60%), Query Frame = 1


Query: 31  GRKMLAMGGDEMLEAQSHIWNHIFNFINSMSLKCVIQLGIPDVIHNHDPNPMPLSLLVSS 90
           G K LAMGGDE+LEAQSHIWNHIFNFINSMSLKC IQLGIPD IH+H PNPMPLSLLVSS
Sbjct: 35  GGKKLAMGGDELLEAQSHIWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPMPLSLLVSS 94

Query: 91  LKLHPNKTTFIYRLMRLLTHSGFFVLQE-GYMLTNSSRLLLKDNHCAVSPFVLSMLEPVL 150
           L+LHPNKT FIYRLMRLLTHSGFFV QE GY+LTNSSRLLLKDNH AVSPF+LSML+P L
Sbjct: 95  LQLHPNKTQFIYRLMRLLTHSGFFVQQEEGYILTNSSRLLLKDNHFAVSPFLLSMLQPAL 154

Query: 151 LEPWQFFSIWFQTDDRTPFETTHGKPFWEYL------GNKPKDGEAWRAM---------- 210
            +PWQF SIW QTDDRTPFET HG PFWEY+      G    +G A  A           
Sbjct: 155 TDPWQFLSIWLQTDDRTPFETAHGMPFWEYMGNKAKDGEVFNEGMASDARLVMSVILEKH 214

Query: 211 ----------------QGTMAKAIAKDFPKIECIVLDLPQVVADLKADQPNFKYVEGNMF 250
                            GTMAKAI++ FP++EC V DLPQVVA LK DQPNFKYVEG+MF
Sbjct: 215 KSVFEGVESLVDVGGGTGTMAKAISQAFPQMECTVFDLPQVVAHLKEDQPNFKYVEGDMF 274

[Close]

BLAST of Lsi11G014050 vs. NCBI nr
Match: gi|778723662|ref|XP_004151735.2| (PREDICTED: trans-resveratrol di-O-methyltransferase [Cucumis sativus])

HSP 1 Score: 331.6 bits (849), Expect = 1.3e-87
Identity = 171/277 (61.73%), Postives = 198/277 (71.48%), Query Frame = 1


Query: 31  GRKMLAMGGDEMLEAQSHIWNHIFNFINSMSLKCVIQLGIPDVIHNHDPNPMPLSLLVSS 90
           G KMLAMGGDE+LEAQSH+WNHIFNFINSMSLKC IQLGIPD IH+H PNP+PLS+LVSS
Sbjct: 5   GGKMLAMGGDELLEAQSHVWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPVPLSILVSS 64

Query: 91  LKLHPNKTTFIYRLMRLLTHSGFFVLQ-EGYMLTNSSRLLLKDNHCAVSPFVLSMLEPVL 150
           L+LHPNKT FIYRLMRLLTHSGFFV Q EGY+LTNSSRLLLKDN CAVSPF+LSML+P L
Sbjct: 65  LQLHPNKTQFIYRLMRLLTHSGFFVQQEEGYILTNSSRLLLKDNPCAVSPFLLSMLQPAL 124

Query: 151 LEPWQFFSIWFQTDDRTPFETTHGKPFWEYLGNKPKDGEAWRAMQGTMAKAI-------- 210
            +PWQF SIWFQTDD+TPFET HG PFW+Y+ NKPK+GE + A   + A+ +        
Sbjct: 125 TDPWQFLSIWFQTDDQTPFETAHGVPFWKYMRNKPKEGEVFNAGMASDARLVINVLLEKH 184

Query: 211 AKDFPKIECIV------------------------LDLPQVVADLKADQPNFKYVEGNMF 270
              F  +E +V                        LDLPQVVA LK DQPNFKYVEG+MF
Sbjct: 185 RSVFEGVESLVDVGGGTGTVAKAISQAFPQMECTVLDLPQVVAHLKGDQPNFKYVEGDMF 244

Query: 271 NVIPPADALSLKLKIRKRMEREKKEWGQLIKEAGFGA 275
            +IPPAD + LK  +    + E  E  +  KEA  G+
Sbjct: 245 TLIPPADTILLKWILHDWSDEECVEILKKCKEAITGS 281

[Close]

BLAST of Lsi11G014050 vs. NCBI nr
Match: gi|778723658|ref|XP_011658687.1| (PREDICTED: trans-resveratrol di-O-methyltransferase-like [Cucumis sativus])

HSP 1 Score: 292.0 bits (746), Expect = 1.2e-75
Identity = 150/253 (59.29%), Postives = 177/253 (69.96%), Query Frame = 1


Query: 31  GRKMLAMGGDEMLEAQSHIWNHIFNFINSMSLKCVIQLGIPDVIHNHDPNPMPLSLLVSS 90
           G  M+ M GDE++EA   I  HIFNFINSMSLKC IQLGIPD IH+H P+P+PLS LVSS
Sbjct: 7   GGNMVEMEGDELMEAHGQICKHIFNFINSMSLKCAIQLGIPDAIHSHGPSPLPLSRLVSS 66

Query: 91  LKLHPNKTTFIYRLMRLLTHSGFFVLQ-EGYMLTNSSRLLLKDNHCAVSPFVLSMLEPVL 150
           L+LHPNKT FIYRLMRLLTHSGFFVLQ EGY+LT SS LLLKDN C +SPF+LSML+PV 
Sbjct: 67  LQLHPNKTQFIYRLMRLLTHSGFFVLQEEGYVLTTSSHLLLKDNSCTLSPFLLSMLQPVF 126

Query: 151 LEPWQFFSIWFQTDDRTPFETTHGKPFWEYLGNKPKDGEAWRAMQGTMAKAI-------- 210
           +EP  F S WF+TDD+TPFET HGK FWE++GNK KDG+ + A   + A+ +        
Sbjct: 127 VEPSHFLSAWFRTDDQTPFETAHGKSFWEFVGNKRKDGDTFNAGMASDARLVMSVLMGKH 186

Query: 211 AKDFPKIECIV------------------------LDLPQVVADLKADQPNFKYVEGNMF 251
              F  +E +V                        LDLPQVVA+LK D PNFKYVEG+MF
Sbjct: 187 TSVFEGVESLVDVGGGTGTMAKAIAKAFPQIECTVLDLPQVVAELKPDIPNFKYVEGDMF 246

The following BLAST results are available for this feature:

BLAST of Lsi11G014050 vs. Swiss-Prot


Match Name	E-value	Identity	Description
ROMT_VITVI	2.5e-57	39.83	Trans-resveratrol di-O-methyltransferase OS=Vitis vinifera GN=ROMT PE=1 SV=2	[more]
CVMT1_OCIBA	1.3e-42	33.33	Chavicol O-methyltransferase OS=Ocimum basilicum GN=CVOMT1 PE=1 SV=1	[more]
Q8OMT_MENPI	2.5e-41	39.35	8-hydroxyquercetin 8-O-methyltransferase OS=Mentha piperita GN=OMT2 PE=1 SV=1	[more]
MOMT_CATRO	3.6e-40	36.65	Myricetin O-methyltransferase OS=Catharanthus roseus PE=1 SV=1	[more]
7OMT9_MEDSA	2.7e-35	32.65	Isoflavone-7-O-methyltransferase 9 OS=Medicago sativa PE=2 SV=1	[more]

BLAST of Lsi11G014050 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K1E9_CUCSA	1.7e-89	52.99	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G039280 PE=3 SV=1	[more]
A0A0A0K5D5_CUCSA	9.3e-88	61.73	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G039790 PE=3 SV=1	[more]
A0A0A0K6Q4_CUCSA	8.2e-76	59.29	O-methyltransferase OS=Cucumis sativus GN=Csa_7G039780 PE=3 SV=1	[more]
F6H5H5_VITVI	8.5e-65	41.74	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_12s0028g02850 PE=3 SV=...	[more]
F6H5F7_VITVI	7.2e-64	42.12	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_12s0028g02950 PE=3 SV=...	[more]

BLAST of Lsi11G014050 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G35160.1	5.3e-18	28.13	O-methyltransferase family protein	[more]
AT3G53140.1	2.7e-09	25.30	O-methyltransferase family protein	[more]

BLAST of Lsi11G014050 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778723655\|ref\|XP_004151089.2\|	2.4e-89	52.99	PREDICTED: trans-resveratrol di-O-methyltransferase-like [Cucumis sativus]	[more]
gi\|700188247\|gb\|KGN43480.1\|	2.4e-89	52.99	hypothetical protein Csa_7G039280 [Cucumis sativus]	[more]
gi\|659110928\|ref\|XP_008455485.1\|	2.4e-89	68.65	PREDICTED: trans-resveratrol di-O-methyltransferase-like [Cucumis melo]	[more]
gi\|778723662\|ref\|XP_004151735.2\|	1.3e-87	61.73	PREDICTED: trans-resveratrol di-O-methyltransferase [Cucumis sativus]	[more]
gi\|778723658\|ref\|XP_011658687.1\|	1.2e-75	59.29	PREDICTED: trans-resveratrol di-O-methyltransferase-like [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0008168	methyltransferase activity
GO:0046983	protein dimerization activity
GO:0008171	O-methyltransferase activity

Vocabulary: **INTERPRO**
Term	Definition
IPR016461	O-MeTrfase_COMT
IPR012967	Plant_MeTrfase_dimerisation
IPR011991	Winged helix-turn-helix DNA-binding domain
IPR001077	O_MeTrfase_2

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0008171	O-methyltransferase activity
molecular_function	GO:0046983	protein dimerization activity
molecular_function	GO:0008168	methyltransferase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi11G014050.1	Lsi11G014050.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001077	O-methyltransferase, family 2	PFAM	PF00891	Methyltransf_2	coord: 195..249 score: 2.8
IPR011991	Winged helix-turn-helix DNA-binding domain	GENE3D	G3DSA:1.10.10.10		coord: 40..129 score: 1.1
IPR011991	Winged helix-turn-helix DNA-binding domain	unknown	SSF46785	"Winged helix" DNA-binding domain	coord: 39..133 score: 9.8
IPR012967	Plant methyltransferase dimerisation	PFAM	PF08100	Dimerisation	coord: 60..109 score: 3.3
IPR016461	O-methyltransferase COMT-type	PROFILE	PS51683	SAM_OMT_II	coord: 50..291 score: 16
None	No IPR available	PANTHER	PTHR11746	O-METHYLTRANSFERASE	coord: 41..291 score: 2.3
None	No IPR available	PANTHER	PTHR11746:SF83	SUBFAMILY NOT NAMED	coord: 41..291 score: 2.3

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi11G014050	MELO3C018858.2	Melon (DHL92) v3.6.1	lsimedB101
Lsi11G014050	CsaV3_7G003170	Cucumber (Chinese Long) v3	cuclsiB529

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi11G014050	Bottle gourd (USVL1VR-Ls)	lsilsiB022
Lsi11G014050	Cucurbita maxima (Rimu)	cmalsiB091
Lsi11G014050	Cucurbita maxima (Rimu)	cmalsiB472
Lsi11G014050	Cucurbita maxima (Rimu)	cmalsiB513
Lsi11G014050	Cucurbita moschata (Rifu)	cmolsiB080
Lsi11G014050	Cucurbita moschata (Rifu)	cmolsiB457
Lsi11G014050	Cucurbita moschata (Rifu)	cmolsiB499
Lsi11G014050	Cucurbita pepo (Zucchini)	cpelsiB199
Lsi11G014050	Cucurbita pepo (Zucchini)	cpelsiB233
Lsi11G014050	Cucurbita pepo (Zucchini)	cpelsiB526
Lsi11G014050	Wild cucumber (PI 183967)	cpilsiB099
Lsi11G014050	Wild cucumber (PI 183967)	cpilsiB505
Lsi11G014050	Wild cucumber (PI 183967)	cpilsiB514
Lsi11G014050	Cucumber (Chinese Long) v2	culsiB099
Lsi11G014050	Cucumber (Chinese Long) v2	culsiB494
Lsi11G014050	Melon (DHL92) v3.5.1	lsimeB088
Lsi11G014050	Melon (DHL92) v3.5.1	lsimeB095
Lsi11G014050	Watermelon (Charleston Gray)	lsiwcgB095
Lsi11G014050	Watermelon (Charleston Gray)	lsiwcgB097
Lsi11G014050	Watermelon (97103) v1	lsiwmB105
Lsi11G014050	Watermelon (97103) v1	lsiwmB106
Lsi11G014050	Cucumber (Gy14) v2	cgyblsiB092
Lsi11G014050	Cucumber (Gy14) v2	cgyblsiB462
Lsi11G014050	Melon (DHL92) v3.6.1	lsimedB094
Lsi11G014050	Silver-seed gourd	carlsiB174
Lsi11G014050	Silver-seed gourd	carlsiB974
Lsi11G014050	Cucumber (Chinese Long) v3	cuclsiB110
Lsi11G014050	Watermelon (97103) v2	lsiwmbB086
Lsi11G014050	Wax gourd	lsiwgoB118
Lsi11G014050	Wax gourd	lsiwgoB127