Lsi11G011180 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi11G011180
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Isoflavone reductase-like protein 5
Location	chr11 : 19607229 .. 19609725 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATGTGGATCGTAGTAATGCTGTAGAGCCAGCAAAATCAGCATTTGGTGTAAAGGTTGAAATTCGTCGAGCTGTGGAGGCAGCAGGAATTCCCTACACTTATGTTTCTTCTAACTTCTTTGCTGGCTACTTCCTTCCTTCCCTGTCTCAGCCTGGAGCTTCTGTTCCACCTAGAGATAAGATTGTTATCTTAGGAGATGGAACTCCGCAATGTAATTTTTCCTTACCCTTTTTCTTTATTTTTACTTTTTTAAGACAGTTGTTGTTTGATAATTGTAGTCGGTCTTGTTGGATAATTATTTGGTTTTTAAAAGATTATAAGTATTTTCTATAAATTGTTATCCATTGTTTTCTATTCTTGTTTTGATTTGAAATCTTAAAAAAAATGATTTTTAAGAAATTTATTTTTGTTTTTAGAATTTGGCTGGAGTTAAAAGTGTGTGTGTTTTTTTAATGGTAGGTGAGATCCTTAATAGAAAGATTAGGAGGAAACAAACATGAGTTTCAAAAACATAAAACGAAAAATGAAGGGTCCTGTTTGATAACCATTTTGTTTTTTATTTTTGATTTTTGAAAATTAAGCTTATTTCTTCACATCTCTTTTTACAATGGTTTGCATCTTTCTTGAATACAAGAGTTGAATTCTTAGCCAAATTCCAAAAAGAAAAATAAATTTTTAAAAACTATTTTTTTTTTTAGTTTTTAAAATTTGGCTTGATTTTTGAACCAGGAGTGAGAGAGGTGTGAATTCTAAGCTAAATTTCAAGAACAAAATCAAGTTTTTAAAAACTAATTTCTTTCGGTGTCAAATTAGACTTGGTTTTATAAATATTTTCAGAAAGTATATATTAATAGTGTTTATAAAGCTTAATTTTCTATAACAAAAAATTAAAATAAAACAAAATATTTATCCAATGAAACTTTAGTATTTGGTTTTTAATTTTTGAAAATGGTGTTATTGTTGCCGAATATGACTTTCATTCTTTCTTAAGGTAAAAATTAAATTCTTAATCAAATTTTAATAAAAATACAAAGAAACACTCATAGGTGGGAGTAAGATTTATAAACTTAAAATTTTAAATTTTAAATCTAAATTATGTTCATATGTACTATAAATATTTCATGAAATAGAGTCTAAGTCTCTTCTGAAATATCTTTAATTTTGTCCTAAGAAAATGTGGTCTCAAAAAGTATGAAAAAAAAAAATTGATCTTATTGCTTTGGTTTCTTGTGAATTATGATCACAGCAATATTCAACAAGGAAGATGACATAGGAGCTTATACAATCAAAGCTGTGGATGATCCAAGAACTTTGAACAAAATTCTTTACATTAGACCAGCTGGTAATATCTACTCTTTCAATGATCTTGTGGCTCTTTGGGAGAAGAAAATTGGGAAAACTGTTGAGAAAATCTATGTTCCTGAGGAACAAGTCTTGAAGAACATTGAAGGTTAGTATTGCATTTTTTTTTTTTTAATTTTGAATCCGTCAATAGCTTTATGAAATTTATCGTAATCCGTCAATTTAGAGCATGTTTGGGAGTGATTTTAAAATCGTCAAAATCACTTTTATCATTTTGAAAATAACTCAAAAACACACTTTTAATCCCTCAAAATTAATTTAGTTTATTCAATTCTACACTTTTAAATGCAATTTTCAATCCTCAAAATTAGTTTTGAACAATTAAAAACATGTTTTATAGTGATTATGAAAATGACAAAAGTAATTTTAGTCATTCGAAAATTATTCCCAAATATGTCCTTGGACTTTTAGATTGAGTGATTTAACATAATATCAAAATAGAAGGTCTTGTCTTAGACCTTCAAACTCCTCCTGCGAAGTCATTTTCTCTAAAGTGAATAGTGATAAATGAGCTACGACTTTCAAAGCACTATTAAAATTTCAAAATATTATTAAGTGATCAAAAGTACTTTTTCATCTACAAAAATCATCAATTTAATCAAAGTTTGTATATAGTAATGATTTGGAATAATTGAAATTTGATTTAAGGCCATAATTACTAATAATAACATTTTTTTTTTTTAATTTTTTGCAGAGGCTCCTCTTCCTGTGAATGTGATATTAGCAATTTCTCACTCAGCATTTGTGAAGGGAGATCATACCAACTTTGAAATTGAACCATCATTTGGAGTGGAAGCTACAAAATTATATCCAGAAGTTAAATATACAACTGTGGATGAATATCTTGATCAGTTTGTTTAAAGCAGTTTGTGGAGGAAAAAAAAATAAGAATAAAGTTTTATCTGCTACTATTTTAATTGAATAAGGAGGGAAAATCAAGGGTTTAAAAATTCAGAAGGAAAAATTTGAAAATGGGTACTACATGCATGTTAGTAGCAGTAGTTAGTTTCATGAAACATTGTTGTTTGTTGTTGTATTTGCTGATTTTCTTATGGTTTGGTTTGTATTTCTTTCTTCATGTGTTCTAAAGATTATGGCAAAATCTATGAAAGTTCATGAAATTTCTCAAAGCA

mRNA sequence

ATGATACCAGCTGGTAATATCTACTCTTTCAATGATCTTGTGGCTCTTTGGGAGAAGAAAATTGGGAAAACTGTTGAGAAAATCTATGTTCCTGAGGAACAAGTCTTGAAGAACATTGAAGAGGCTCCTCTTCCTGTGAATGTGATATTAGCAATTTCTCACTCAGCATTTGTGAAGGGAGATCATACCAACTTTGAAATTGAACCATCATTTGGAGTGGAAGCTACAAAATTATATCCAGAAGTTAAATATACAACTGTGGATGAATATCTTGATCAGTTTGTTTAAAGCAGTTTGTGGAGGAAAAAAAAATAAGAATAAAGTTTTATCTGCTACTATTTTAATTGAATAAGGAGGGAAAATCAAGGGTTTAAAAATTCAGAAGGAAAAATTTGAAAATGGGTACTACATGCATGTTAGTAGCAGTAGTTAGTTTCATGAAACATTGTTGTTTGTTGTTGTATTTGCTGATTTTCTTATGGTTTGGTTTGTATTTCTTTCTTCATGTGTTCTAAAGATTATGGCAAAATCTATGAAAGTTCATGAAATTTCTCAAAGCA

Coding sequence (CDS)

ATGATACCAGCTGGTAATATCTACTCTTTCAATGATCTTGTGGCTCTTTGGGAGAAGAAAATTGGGAAAACTGTTGAGAAAATCTATGTTCCTGAGGAACAAGTCTTGAAGAACATTGAAGAGGCTCCTCTTCCTGTGAATGTGATATTAGCAATTTCTCACTCAGCATTTGTGAAGGGAGATCATACCAACTTTGAAATTGAACCATCATTTGGAGTGGAAGCTACAAAATTATATCCAGAAGTTAAATATACAACTGTGGATGAATATCTTGATCAGTTTGTTTAA

Protein sequence

MIPAGNIYSFNDLVALWEKKIGKTVEKIYVPEEQVLKNIEEAPLPVNVILAISHSAFVKGDHTNFEIEPSFGVEATKLYPEVKYTTVDEYLDQFV

Homology

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BLAST of Lsi11G011180 vs. Swiss-Prot
Match: ALL12_OLEEU (Isoflavone reductase-like protein OS=Olea europaea PE=1 SV=1)

HSP 1 Score: 162.5 bits (410), Expect = 2.2e-39
Identity = 74/93 (79.57%), Postives = 88/93 (94.62%), Query Frame = 1


Query: 3   PAGNIYSFNDLVALWEKKIGKTVEKIYVPEEQVLKNIEEAPLPVNVILAISHSAFVKGDH 62
           P  NIYSFN+LVALWEKKIGKT+EKIYVPEEQVLK I+E+P P+N+++AI+HSAFVKGD 
Sbjct: 216 PPKNIYSFNELVALWEKKIGKTLEKIYVPEEQVLKQIQESPFPINIVMAINHSAFVKGDL 275

Query: 63  TNFEIEPSFGVEATKLYPEVKYTTVDEYLDQFV 96
           TNF+IEPSFGVEA++LYP+VKYTTV+EYLDQFV
Sbjct: 276 TNFKIEPSFGVEASELYPDVKYTTVEEYLDQFV 308

[Close]

BLAST of Lsi11G011180 vs. Swiss-Prot
Match: IFRH_SOLTU (Isoflavone reductase homolog OS=Solanum tuberosum PE=2 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 5.3e-33
Identity = 64/93 (68.82%), Postives = 82/93 (88.17%), Query Frame = 1


Query: 3   PAGNIYSFNDLVALWEKKIGKTVEKIYVPEEQVLKNIEEAPLPVNVILAISHSAFVKGDH 62
           P  NI + N+LV+LWEKK GK +E++YVPEEQVLKNI+EA +P+NV L+I H+AFVKGDH
Sbjct: 216 PPHNIITLNELVSLWEKKTGKNLERLYVPEEQVLKNIQEASVPMNVGLSIYHTAFVKGDH 275

Query: 63  TNFEIEPSFGVEATKLYPEVKYTTVDEYLDQFV 96
           TNFEIEPSFGVEA+++YP+VKYT +DE L+Q+V
Sbjct: 276 TNFEIEPSFGVEASEVYPDVKYTPIDEILNQYV 308

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BLAST of Lsi11G011180 vs. Swiss-Prot
Match: IFRH_ARATH (Isoflavone reductase homolog P3 OS=Arabidopsis thaliana GN=At1g75280 PE=2 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 5.4e-30
Identity = 58/92 (63.04%), Postives = 79/92 (85.87%), Query Frame = 1


Query: 3   PAGNIYSFNDLVALWEKKIGKTVEKIYVPEEQVLKNIEEAPLPVNVILAISHSAFVKGDH 62
           P+ N  S N++V LWEKKIGK++EK ++PEEQ+LK+I+E+P+P+NV+L+I+H+ FV GD 
Sbjct: 219 PSNNTLSMNEIVTLWEKKIGKSLEKTHLPEEQLLKSIQESPIPINVVLSINHAVFVNGD- 278

Query: 63  TNFEIEPSFGVEATKLYPEVKYTTVDEYLDQF 95
           TN  IEPSFGVEA++LYP+VKYT+VDEYL  F
Sbjct: 279 TNISIEPSFGVEASELYPDVKYTSVDEYLSYF 309

[Close]

BLAST of Lsi11G011180 vs. Swiss-Prot
Match: IFRH_MAIZE (Isoflavone reductase homolog IRL OS=Zea mays GN=IRL PE=2 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 1.6e-29
Identity = 57/93 (61.29%), Postives = 78/93 (83.87%), Query Frame = 1


Query: 3   PAGNIYSFNDLVALWEKKIGKTVEKIYVPEEQVLKNIEEAPLPVNVILAISHSAFVKGDH 62
           P  N  S N+L++LWEKK GKT  + YVPEE VLK I+E+P+P+N+ILAI H+AFV+G+ 
Sbjct: 217 PPANTLSHNELLSLWEKKTGKTFRREYVPEEAVLKQIQESPIPLNIILAIGHAAFVRGEQ 276

Query: 63  TNFEIEPSFGVEATKLYPEVKYTTVDEYLDQFV 96
           T FEI+P+ GV+A++LYP+VKYTTVDEYL++F+
Sbjct: 277 TGFEIDPAKGVDASELYPDVKYTTVDEYLNRFL 309

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BLAST of Lsi11G011180 vs. Swiss-Prot
Match: IFRH_TOBAC (Isoflavone reductase homolog A622 OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 1.0e-28
Identity = 57/95 (60.00%), Postives = 75/95 (78.95%), Query Frame = 1


Query: 1   MIPAGNIYSFNDLVALWEKKIGKTVEKIYVPEEQVLKNIEEAPLPVNVILAISHSAFVKG 60
           M P  NI SFN++V+LWE KIGKT+EK+Y+ EE +L+ ++E PLP+   LAI HS FV G
Sbjct: 216 MRPPANILSFNEIVSLWEDKIGKTLEKLYLSEEDILQIVQEGPLPLRTNLAICHSVFVNG 275

Query: 61  DHTNFEIEPSFGVEATKLYPEVKYTTVDEYLDQFV 96
           D  NFE++P  GVEAT+LYP+VKYTTVDE+ ++FV
Sbjct: 276 DSANFEVQPPTGVEATELYPKVKYTTVDEFYNKFV 310

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BLAST of Lsi11G011180 vs. TrEMBL
Match: A0A0A0K4D9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G004020 PE=4 SV=1)

HSP 1 Score: 188.0 bits (476), Expect = 5.5e-45
Identity = 91/93 (97.85%), Postives = 93/93 (100.00%), Query Frame = 1


Query: 3   PAGNIYSFNDLVALWEKKIGKTVEKIYVPEEQVLKNIEEAPLPVNVILAISHSAFVKGDH 62
           PAGNIYSFNDLVALWEKKIGK+VEKIYVPEEQVLKNIEEAPLPVNVILAISHSAFVKGDH
Sbjct: 216 PAGNIYSFNDLVALWEKKIGKSVEKIYVPEEQVLKNIEEAPLPVNVILAISHSAFVKGDH 275

Query: 63  TNFEIEPSFGVEATKLYPEVKYTTVDEYLDQFV 96
           TNFEIEPSFGVEATKLYPEVKYT+VDEYLDQFV
Sbjct: 276 TNFEIEPSFGVEATKLYPEVKYTSVDEYLDQFV 308

[Close]

BLAST of Lsi11G011180 vs. TrEMBL
Match: B2WSN0_CLABR (Eugenol synthase 2 OS=Clarkia breweri PE=2 SV=1)

HSP 1 Score: 169.5 bits (428), Expect = 2.0e-39
Identity = 76/93 (81.72%), Postives = 89/93 (95.70%), Query Frame = 1


Query: 3   PAGNIYSFNDLVALWEKKIGKTVEKIYVPEEQVLKNIEEAPLPVNVILAISHSAFVKGDH 62
           P+ NIYSFN+LVALWEKKIGKT+EKIYVPEEQ+LK+I+EAP+P+N+ L I+HS FVKGDH
Sbjct: 217 PSNNIYSFNELVALWEKKIGKTLEKIYVPEEQILKDIQEAPIPINIFLGINHSVFVKGDH 276

Query: 63  TNFEIEPSFGVEATKLYPEVKYTTVDEYLDQFV 96
           TNFEIEPSFGVEA++LYPEVKYTTV+EYLDQFV
Sbjct: 277 TNFEIEPSFGVEASELYPEVKYTTVEEYLDQFV 309

[Close]

BLAST of Lsi11G011180 vs. TrEMBL
Match: A0A0A0K4D3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G070260 PE=4 SV=1)

HSP 1 Score: 169.5 bits (428), Expect = 2.0e-39
Identity = 76/93 (81.72%), Postives = 91/93 (97.85%), Query Frame = 1


Query: 3   PAGNIYSFNDLVALWEKKIGKTVEKIYVPEEQVLKNIEEAPLPVNVILAISHSAFVKGDH 62
           P+GN YSFNDLV+LWEKKIGK +E+IYVPEEQVLKNI+EAP+P+N+I++ISHSAFVKGDH
Sbjct: 216 PSGNTYSFNDLVSLWEKKIGKNLERIYVPEEQVLKNIQEAPVPLNIIISISHSAFVKGDH 275

Query: 63  TNFEIEPSFGVEATKLYPEVKYTTVDEYLDQFV 96
           TNF+IEPSFGVEAT+LYP+VKYTTVDE+L+QFV
Sbjct: 276 TNFDIEPSFGVEATQLYPDVKYTTVDEFLNQFV 308

[Close]

BLAST of Lsi11G011180 vs. TrEMBL
Match: Q3KN68_VITVI (Isoflavone reductase-like protein 5 OS=Vitis vinifera GN=ifrl5 PE=2 SV=1)

HSP 1 Score: 167.5 bits (423), Expect = 7.6e-39
Identity = 78/93 (83.87%), Postives = 88/93 (94.62%), Query Frame = 1


Query: 3   PAGNIYSFNDLVALWEKKIGKTVEKIYVPEEQVLKNIEEAPLPVNVILAISHSAFVKGDH 62
           P  N YSFNDLV+LWEKKIGKT+EKIYVPEEQVLKNI+EA +P+NVIL+ISHS F+KGDH
Sbjct: 214 PPQNTYSFNDLVSLWEKKIGKTLEKIYVPEEQVLKNIQEASVPLNVILSISHSVFIKGDH 273

Query: 63  TNFEIEPSFGVEATKLYPEVKYTTVDEYLDQFV 96
           TNFEIEPSFGVEAT+LYP+VKYTTVDEYL+QFV
Sbjct: 274 TNFEIEPSFGVEATELYPDVKYTTVDEYLNQFV 306

[Close]

BLAST of Lsi11G011180 vs. TrEMBL
Match: D7U4J6_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_03s0038g04670 PE=4 SV=1)

HSP 1 Score: 166.4 bits (420), Expect = 1.7e-38
Identity = 77/93 (82.80%), Postives = 88/93 (94.62%), Query Frame = 1


Query: 3   PAGNIYSFNDLVALWEKKIGKTVEKIYVPEEQVLKNIEEAPLPVNVILAISHSAFVKGDH 62
           P  N YSFNDLV+LWEKKIGKT+EKIYVPEEQVLKNI+EA +P+NVIL+ISHS F+KGDH
Sbjct: 214 PPQNTYSFNDLVSLWEKKIGKTLEKIYVPEEQVLKNIQEASVPLNVILSISHSVFIKGDH 273

Query: 63  TNFEIEPSFGVEATKLYPEVKYTTVDEYLDQFV 96
           TNFEI+PSFGVEAT+LYP+VKYTTVDEYL+QFV
Sbjct: 274 TNFEIQPSFGVEATELYPDVKYTTVDEYLNQFV 306

[Close]

BLAST of Lsi11G011180 vs. TAIR10
Match: AT4G39230.1 (AT4G39230.1 NmrA-like negative transcriptional regulator family protein)

HSP 1 Score: 140.2 bits (352), Expect = 6.6e-34
Identity = 65/93 (69.89%), Postives = 80/93 (86.02%), Query Frame = 1


Query: 3   PAGNIYSFNDLVALWEKKIGKTVEKIYVPEEQVLKNIEEAPLPVNVILAISHSAFVKGDH 62
           P  N YSFNDLV+LWE KIGKT+E+IYVPEEQ+LK I E+  P+NV+L++ H  FVKG H
Sbjct: 216 PPMNTYSFNDLVSLWENKIGKTLERIYVPEEQLLKQIIESSPPLNVMLSLCHCVFVKGGH 275

Query: 63  TNFEIEPSFGVEATKLYPEVKYTTVDEYLDQFV 96
           T+FEIEPSFGVEA++LYP+VKYTTVDE L+Q+V
Sbjct: 276 TSFEIEPSFGVEASELYPDVKYTTVDEILNQYV 308

[Close]

BLAST of Lsi11G011180 vs. TAIR10
Match: AT1G75290.1 (AT1G75290.1 NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 135.2 bits (339), Expect = 2.1e-32
Identity = 58/92 (63.04%), Postives = 77/92 (83.70%), Query Frame = 1


Query: 3   PAGNIYSFNDLVALWEKKIGKTVEKIYVPEEQVLKNIEEAPLPVNVILAISHSAFVKGDH 62
           P  N  S N++V LWEKKIGK+VEKIY+ EEQ+ K+I+E+P+P NV+L+I+H+ FVKGD 
Sbjct: 219 PPNNTLSMNEIVTLWEKKIGKSVEKIYMSEEQIFKSIQESPVPFNVLLSINHAVFVKGDQ 278

Query: 63  TNFEIEPSFGVEATKLYPEVKYTTVDEYLDQF 95
           TNF IEPSFG EA++LYP++KYT++DEYL  F
Sbjct: 279 TNFTIEPSFGFEASELYPDIKYTSIDEYLSYF 310

[Close]

BLAST of Lsi11G011180 vs. TAIR10
Match: AT1G75280.1 (AT1G75280.1 NmrA-like negative transcriptional regulator family protein)

HSP 1 Score: 131.3 bits (329), Expect = 3.1e-31
Identity = 58/92 (63.04%), Postives = 79/92 (85.87%), Query Frame = 1


Query: 3   PAGNIYSFNDLVALWEKKIGKTVEKIYVPEEQVLKNIEEAPLPVNVILAISHSAFVKGDH 62
           P+ N  S N++V LWEKKIGK++EK ++PEEQ+LK+I+E+P+P+NV+L+I+H+ FV GD 
Sbjct: 219 PSNNTLSMNEIVTLWEKKIGKSLEKTHLPEEQLLKSIQESPIPINVVLSINHAVFVNGD- 278

Query: 63  TNFEIEPSFGVEATKLYPEVKYTTVDEYLDQF 95
           TN  IEPSFGVEA++LYP+VKYT+VDEYL  F
Sbjct: 279 TNISIEPSFGVEASELYPDVKYTSVDEYLSYF 309

[Close]

BLAST of Lsi11G011180 vs. TAIR10
Match: AT1G75300.1 (AT1G75300.1 NmrA-like negative transcriptional regulator family protein)

HSP 1 Score: 117.1 bits (292), Expect = 6.0e-27
Identity = 53/92 (57.61%), Postives = 73/92 (79.35%), Query Frame = 1


Query: 3   PAGNIYSFNDLVALWEKKIGKTVEKIYVPEEQVLKNIEEAPLPVNVILAISHSAFVKGDH 62
           P  NI S N++V LWEKKIGK++EK ++ EEQ+LK+I+   +P++V  +I+H+ FVKGD 
Sbjct: 233 PPNNILSMNEMVTLWEKKIGKSLEKTHISEEQILKSIQ---VPIDVFKSINHAVFVKGDQ 292

Query: 63  TNFEIEPSFGVEATKLYPEVKYTTVDEYLDQF 95
           T+F IEP FG EA+ LYP+VKYT++DEYL QF
Sbjct: 293 TSFTIEPWFGEEASVLYPDVKYTSIDEYLSQF 321

[Close]

BLAST of Lsi11G011180 vs. TAIR10
Match: AT1G19540.1 (AT1G19540.1 NmrA-like negative transcriptional regulator family protein)

HSP 1 Score: 116.3 bits (290), Expect = 1.0e-26
Identity = 52/93 (55.91%), Postives = 74/93 (79.57%), Query Frame = 1


Query: 3   PAGNIYSFNDLVALWEKKIGKTVEKIYVPEEQVLKNIEEAPLPVNVILAISHSAFVKGDH 62
           P   I S ND+V LWE+KIGKT+EK YV EE++LK I+E+  P++ ++ + H+  VK D 
Sbjct: 218 PPNYIVSQNDMVGLWEEKIGKTLEKTYVSEEELLKTIQESKPPMDFLVGLIHTILVKSDF 277

Query: 63  TNFEIEPSFGVEATKLYPEVKYTTVDEYLDQFV 96
           T+F I+PSFGVEA++LYPEVKYT+VDE+L++F+
Sbjct: 278 TSFTIDPSFGVEASELYPEVKYTSVDEFLNRFI 310

[Close]

BLAST of Lsi11G011180 vs. NCBI nr
Match: gi|449461591|ref|XP_004148525.1| (PREDICTED: isoflavone reductase-like protein [Cucumis sativus])

HSP 1 Score: 188.0 bits (476), Expect = 7.8e-45
Identity = 91/93 (97.85%), Postives = 93/93 (100.00%), Query Frame = 1


Query: 3   PAGNIYSFNDLVALWEKKIGKTVEKIYVPEEQVLKNIEEAPLPVNVILAISHSAFVKGDH 62
           PAGNIYSFNDLVALWEKKIGK+VEKIYVPEEQVLKNIEEAPLPVNVILAISHSAFVKGDH
Sbjct: 216 PAGNIYSFNDLVALWEKKIGKSVEKIYVPEEQVLKNIEEAPLPVNVILAISHSAFVKGDH 275

Query: 63  TNFEIEPSFGVEATKLYPEVKYTTVDEYLDQFV 96
           TNFEIEPSFGVEATKLYPEVKYT+VDEYLDQFV
Sbjct: 276 TNFEIEPSFGVEATKLYPEVKYTSVDEYLDQFV 308

[Close]

BLAST of Lsi11G011180 vs. NCBI nr
Match: gi|659094478|ref|XP_008448082.1| (PREDICTED: isoflavone reductase-like protein [Cucumis melo])

HSP 1 Score: 183.7 bits (465), Expect = 1.5e-43
Identity = 89/93 (95.70%), Postives = 92/93 (98.92%), Query Frame = 1


Query: 3   PAGNIYSFNDLVALWEKKIGKTVEKIYVPEEQVLKNIEEAPLPVNVILAISHSAFVKGDH 62
           PAGNIYSFNDLVALWEKKIGK+VEKIYVPEEQVLKNIEEAPLP+NVILAISHSAFVKGDH
Sbjct: 216 PAGNIYSFNDLVALWEKKIGKSVEKIYVPEEQVLKNIEEAPLPLNVILAISHSAFVKGDH 275

Query: 63  TNFEIEPSFGVEATKLYPEVKYTTVDEYLDQFV 96
           TNFEIE SFGVEATKLYPEVKYT+VDEYLDQFV
Sbjct: 276 TNFEIEASFGVEATKLYPEVKYTSVDEYLDQFV 308

[Close]

BLAST of Lsi11G011180 vs. NCBI nr
Match: gi|149349524|gb|ABR24114.1| (eugenol synthase 2 [Clarkia breweri])

HSP 1 Score: 169.5 bits (428), Expect = 2.9e-39
Identity = 76/93 (81.72%), Postives = 89/93 (95.70%), Query Frame = 1


Query: 3   PAGNIYSFNDLVALWEKKIGKTVEKIYVPEEQVLKNIEEAPLPVNVILAISHSAFVKGDH 62
           P+ NIYSFN+LVALWEKKIGKT+EKIYVPEEQ+LK+I+EAP+P+N+ L I+HS FVKGDH
Sbjct: 217 PSNNIYSFNELVALWEKKIGKTLEKIYVPEEQILKDIQEAPIPINIFLGINHSVFVKGDH 276

Query: 63  TNFEIEPSFGVEATKLYPEVKYTTVDEYLDQFV 96
           TNFEIEPSFGVEA++LYPEVKYTTV+EYLDQFV
Sbjct: 277 TNFEIEPSFGVEASELYPEVKYTTVEEYLDQFV 309

[Close]

BLAST of Lsi11G011180 vs. NCBI nr
Match: gi|449438299|ref|XP_004136926.1| (PREDICTED: isoflavone reductase-like protein [Cucumis sativus])

HSP 1 Score: 169.5 bits (428), Expect = 2.9e-39
Identity = 76/93 (81.72%), Postives = 91/93 (97.85%), Query Frame = 1


Query: 3   PAGNIYSFNDLVALWEKKIGKTVEKIYVPEEQVLKNIEEAPLPVNVILAISHSAFVKGDH 62
           P+GN YSFNDLV+LWEKKIGK +E+IYVPEEQVLKNI+EAP+P+N+I++ISHSAFVKGDH
Sbjct: 216 PSGNTYSFNDLVSLWEKKIGKNLERIYVPEEQVLKNIQEAPVPLNIIISISHSAFVKGDH 275

Query: 63  TNFEIEPSFGVEATKLYPEVKYTTVDEYLDQFV 96
           TNF+IEPSFGVEAT+LYP+VKYTTVDE+L+QFV
Sbjct: 276 TNFDIEPSFGVEATQLYPDVKYTTVDEFLNQFV 308

[Close]

BLAST of Lsi11G011180 vs. NCBI nr
Match: gi|720024415|ref|XP_010263625.1| (PREDICTED: isoflavone reductase-like protein [Nelumbo nucifera])

HSP 1 Score: 167.9 bits (424), Expect = 8.4e-39
Identity = 74/93 (79.57%), Postives = 92/93 (98.92%), Query Frame = 1


Query: 3   PAGNIYSFNDLVALWEKKIGKTVEKIYVPEEQVLKNIEEAPLPVNVILAISHSAFVKGDH 62
           PAGNIYSFND+V+LWEKKIGKT+E+IYVPEE++LK+I+E+P+P+NV+L+ISHS FVKGDH
Sbjct: 216 PAGNIYSFNDIVSLWEKKIGKTLERIYVPEEKLLKDIQESPIPINVVLSISHSVFVKGDH 275

Query: 63  TNFEIEPSFGVEATKLYPEVKYTTVDEYLDQFV 96
           TNFEIEPSFGVEA++LYP+VKYT+VDEYL+QFV
Sbjct: 276 TNFEIEPSFGVEASELYPDVKYTSVDEYLNQFV 308

The following BLAST results are available for this feature:

BLAST of Lsi11G011180 vs. Swiss-Prot


Match Name	E-value	Identity	Description
ALL12_OLEEU	2.2e-39	79.57	Isoflavone reductase-like protein OS=Olea europaea PE=1 SV=1	[more]
IFRH_SOLTU	5.3e-33	68.82	Isoflavone reductase homolog OS=Solanum tuberosum PE=2 SV=1	[more]
IFRH_ARATH	5.4e-30	63.04	Isoflavone reductase homolog P3 OS=Arabidopsis thaliana GN=At1g75280 PE=2 SV=1	[more]
IFRH_MAIZE	1.6e-29	61.29	Isoflavone reductase homolog IRL OS=Zea mays GN=IRL PE=2 SV=1	[more]
IFRH_TOBAC	1.0e-28	60.00	Isoflavone reductase homolog A622 OS=Nicotiana tabacum PE=2 SV=1	[more]

BLAST of Lsi11G011180 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K4D9_CUCSA	5.5e-45	97.85	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G004020 PE=4 SV=1	[more]
B2WSN0_CLABR	2.0e-39	81.72	Eugenol synthase 2 OS=Clarkia breweri PE=2 SV=1	[more]
A0A0A0K4D3_CUCSA	2.0e-39	81.72	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G070260 PE=4 SV=1	[more]
Q3KN68_VITVI	7.6e-39	83.87	Isoflavone reductase-like protein 5 OS=Vitis vinifera GN=ifrl5 PE=2 SV=1	[more]
D7U4J6_VITVI	1.7e-38	82.80	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_03s0038g04670 PE=4 SV=...	[more]

BLAST of Lsi11G011180 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G39230.1	6.6e-34	69.89	NmrA-like negative transcriptional regulator family protein	[more]
AT1G75290.1	2.1e-32	63.04	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT1G75280.1	3.1e-31	63.04	NmrA-like negative transcriptional regulator family protein	[more]
AT1G75300.1	6.0e-27	57.61	NmrA-like negative transcriptional regulator family protein	[more]
AT1G19540.1	1.0e-26	55.91	NmrA-like negative transcriptional regulator family protein	[more]

BLAST of Lsi11G011180 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449461591\|ref\|XP_004148525.1\|	7.8e-45	97.85	PREDICTED: isoflavone reductase-like protein [Cucumis sativus]	[more]
gi\|659094478\|ref\|XP_008448082.1\|	1.5e-43	95.70	PREDICTED: isoflavone reductase-like protein [Cucumis melo]	[more]
gi\|149349524\|gb\|ABR24114.1\|	2.9e-39	81.72	eugenol synthase 2 [Clarkia breweri]	[more]
gi\|449438299\|ref\|XP_004136926.1\|	2.9e-39	81.72	PREDICTED: isoflavone reductase-like protein [Cucumis sativus]	[more]
gi\|720024415\|ref\|XP_010263625.1\|	8.4e-39	79.57	PREDICTED: isoflavone reductase-like protein [Nelumbo nucifera]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR016040	NAD(P)-bd_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi11G011180.1	Lsi11G011180.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016040	NAD(P)-binding domain	unknown	SSF51735	NAD(P)-binding Rossmann-fold domains	coord: 4..65 score: 5.0
None	No IPR available	GENE3D	G3DSA:3.90.25.10		coord: 3..95 score: 1.6
None	No IPR available	PANTHER	PTHR10366	NAD DEPENDENT EPIMERASE/DEHYDRATASE	coord: 3..33 score: 1.1E-38coord: 55..95 score: 1.1
None	No IPR available	PANTHER	PTHR10366:SF375	SUBFAMILY NOT NAMED	coord: 3..33 score: 1.1E-38coord: 55..95 score: 1.1

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi11G011180	ClCG02G004800	Watermelon (Charleston Gray)	lsiwcgB097
Lsi11G011180	MELO3C023304.2	Melon (DHL92) v3.6.1	lsimedB090
Lsi11G011180	Cla97C11G211050	Watermelon (97103) v2	lsiwmbB080
Lsi11G011180	Bhi05G001118	Wax gourd	lsiwgoB115

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi11G011180	Cucurbita pepo (Zucchini)	cpelsiB662
Lsi11G011180	Melon (DHL92) v3.5.1	lsimeB086
Lsi11G011180	Wild cucumber (PI 183967)	cpilsiB097
Lsi11G011180	Wild cucumber (PI 183967)	cpilsiB506
Lsi11G011180	Cucumber (Chinese Long) v2	culsiB494
Lsi11G011180	Melon (DHL92) v3.5.1	lsimeB096
Lsi11G011180	Watermelon (Charleston Gray)	lsiwcgB085
Lsi11G011180	Watermelon (97103) v1	lsiwmB106
Lsi11G011180	Watermelon (97103) v1	lsiwmB111
Lsi11G011180	Cucumber (Gy14) v2	cgyblsiB090
Lsi11G011180	Cucumber (Gy14) v2	cgyblsiB462
Lsi11G011180	Melon (DHL92) v3.6.1	lsimedB102
Lsi11G011180	Silver-seed gourd	carlsiB157
Lsi11G011180	Silver-seed gourd	carlsiB290
Lsi11G011180	Silver-seed gourd	carlsiB891
Lsi11G011180	Cucumber (Chinese Long) v3	cuclsiB109
Lsi11G011180	Cucumber (Chinese Long) v3	cuclsiB529
Lsi11G011180	Watermelon (97103) v2	lsiwmbB086
Lsi11G011180	Wax gourd	lsiwgoB128
Lsi11G011180	Bottle gourd (USVL1VR-Ls)	lsilsiB021
Lsi11G011180	Cucumber (Gy14) v1	cgylsiB146
Lsi11G011180	Cucumber (Gy14) v1	cgylsiB610
Lsi11G011180	Cucurbita maxima (Rimu)	cmalsiB103
Lsi11G011180	Cucurbita maxima (Rimu)	cmalsiB472
Lsi11G011180	Cucurbita maxima (Rimu)	cmalsiB514
Lsi11G011180	Cucurbita maxima (Rimu)	cmalsiB562
Lsi11G011180	Cucurbita moschata (Rifu)	cmolsiB090
Lsi11G011180	Cucurbita moschata (Rifu)	cmolsiB457
Lsi11G011180	Cucurbita moschata (Rifu)	cmolsiB500
Lsi11G011180	Cucurbita moschata (Rifu)	cmolsiB545
Lsi11G011180	Cucurbita pepo (Zucchini)	cpelsiB199
Lsi11G011180	Cucurbita pepo (Zucchini)	cpelsiB231
Lsi11G011180	Cucurbita pepo (Zucchini)	cpelsiB532
Lsi11G011180	Cucurbita pepo (Zucchini)	cpelsiB605