Lsi11G010630 (gene) Bottle gourd (USVL1VR-Ls)

Overview
NameLsi11G010630
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionRibonuclease 1-like protein
Locationchr11 : 18460401 .. 18461092 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCTTGGTTGCAATAGGTTTGTTTTCAAAATATGATGAAGAAAAAAACTCTTCTCTTCACCATCCTTCTTCTCATTCTTATTCGAGTTCAAGGCCAGGTTTTTACCCACTTCTTTTTTGTTCAACAATGGCCGGAGGCCCTTTGTAAAATACAACCATGCGATGTCGATAATGTACCAACGAAATTCACTATTCATGGCCTCTGGCCTCAAAATGGTCAATCCTACCTGATTGATTGTGATTCTAATTTCGAAGCTGATAAGGTAAATCTAAACACTCTCTAAATCTCAAATTAATTGTGATTCTAATTTTTTTTTTTTTCTCAGATTTCACATCTTACAGCTCAATTGAGTGTAGAATGGCCAAACTTATTAGATAGACATGGGTTTGAATGGTTCTGGACATACGAGTGGAACAAGCACGGCACTTGCTCACAAACTCAATTCAGCCAAGCCATGTACTTCCAGTTGGCTTTGAATATGAAGAAAAATGTTGACTTAGTTAGTGCCCTTGAAAAGGGTGGGATTGTCCCTGACGATAGCCTCAAACTGAAGAAAACGGTGCAAGCCGTACTTAAAGCCGAACTTGGGAAGGAACCCTTTCTCCGTTGTAAAAATGATTCCACCCAAACAGCCCGATTGGTGGAGATGCTTAATTATAATGAAATTTGGTTCTAAATGACGCCAATTGCTTA

mRNA sequence

CCTTGGTTGCAATAGGTTTGTTTTCAAAATATGATGAAGAAAAAAACTCTTCTCTTCACCATCCTTCTTCTCATTCTTATTCGAGTTCAAGGCCAGGTTTTTACCCACTTCTTTTTTGTTCAACAATGGCCGGAGGCCCTTTGTAAAATACAACCATGCGATGTCGATAATGTACCAACGAAATTCACTATTCATGGCCTCTGGCCTCAAAATGGTCAATCCTACCTGATTGATTGTGATTCTAATTTCGAAGCTGATAAGATTTCACATCTTACAGCTCAATTGAGTGTAGAATGGCCAAACTTATTAGATAGACATGGGTTTGAATGGTTCTGGACATACGAGTGGAACAAGCACGGCACTTGCTCACAAACTCAATTCAGCCAAGCCATGTACTTCCAGTTGGCTTTGAATATGAAGAAAAATGTTGACTTAGTTAGTGCCCTTGAAAAGGGTGGGATTGTCCCTGACGATAGCCTCAAACTGAAGAAAACGGTGCAAGCCGTACTTAAAGCCGAACTTGGGAAGGAACCCTTTCTCCGTTGTAAAAATGATTCCACCCAAACAGCCCGATTGGTGGAGATGCTTAATTATAATGAAATTTGGTTCTAAATGACGCCAATTGCTTA

Coding sequence (CDS)

ATGATGAAGAAAAAAACTCTTCTCTTCACCATCCTTCTTCTCATTCTTATTCGAGTTCAAGGCCAGGTTTTTACCCACTTCTTTTTTGTTCAACAATGGCCGGAGGCCCTTTGTAAAATACAACCATGCGATGTCGATAATGTACCAACGAAATTCACTATTCATGGCCTCTGGCCTCAAAATGGTCAATCCTACCTGATTGATTGTGATTCTAATTTCGAAGCTGATAAGATTTCACATCTTACAGCTCAATTGAGTGTAGAATGGCCAAACTTATTAGATAGACATGGGTTTGAATGGTTCTGGACATACGAGTGGAACAAGCACGGCACTTGCTCACAAACTCAATTCAGCCAAGCCATGTACTTCCAGTTGGCTTTGAATATGAAGAAAAATGTTGACTTAGTTAGTGCCCTTGAAAAGGGTGGGATTGTCCCTGACGATAGCCTCAAACTGAAGAAAACGGTGCAAGCCGTACTTAAAGCCGAACTTGGGAAGGAACCCTTTCTCCGTTGTAAAAATGATTCCACCCAAACAGCCCGATTGGTGGAGATGCTTAATTATAATGAAATTTGGTTCTAA

Protein sequence

MMKKKTLLFTILLLILIRVQGQVFTHFFFVQQWPEALCKIQPCDVDNVPTKFTIHGLWPQNGQSYLIDCDSNFEADKISHLTAQLSVEWPNLLDRHGFEWFWTYEWNKHGTCSQTQFSQAMYFQLALNMKKNVDLVSALEKGGIVPDDSLKLKKTVQAVLKAELGKEPFLRCKNDSTQTARLVEMLNYNEIWF
Homology
[Close]
BLAST of Lsi11G010630 vs. Swiss-Prot
Match: RNMC_MOMCH (Ribonuclease MC OS=Momordica charantia PE=1 SV=1)

HSP 1 Score: 149.4 bits (376), Expect = 3.9e-35
Identity = 75/169 (44.38%), Postives = 108/169 (63.91%), Query Frame = 1 

Query: 24  FTHFFFVQQWPEALCKIQP---CDVDNVPTKFTIHGLWPQNGQSYLIDCD--SNFEADKI 83
           F  F+FVQQWP A+C  Q    C    + T FTIHGLWPQ   + L +C   S F+  KI
Sbjct: 1   FDSFWFVQQWPPAVCSFQKSGSCPGSGLRT-FTIHGLWPQGSGTSLTNCPQGSPFDITKI 60

Query: 84  SHLTAQLSVEWPNLLDRHGFEWFWTYEWNKHGTCSQTQFSQAMYFQLALNMKKNVDLVSA 143
           SHL +QL+  WPN+L R   + FW++EW KHGTCS++ F+QA YF+LA++M+ N D++ A
Sbjct: 61  SHLQSQLNTLWPNVL-RANNQQFWSHEWTKHGTCSESTFNQAAYFKLAVDMRNNYDIIGA 120

Query: 144 LEKGGIVPDDSLKLKKTVQAVLKAELGKEPFLRCKND-STQTARLVEML 187
           L      P+   K ++ ++  LKA+ GK P LRC+ D  T+ + LV+++
Sbjct: 121 LRPHAAGPNGRTKSRQAIKGFLKAKFGKFPGLRCRTDPQTKVSYLVQVV 167

[Close]
BLAST of Lsi11G010630 vs. Swiss-Prot
Match: RNS1_ARATH (Ribonuclease 1 OS=Arabidopsis thaliana GN=RNS1 PE=1 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 2.2e-25
Identity = 69/198 (34.85%), Postives = 104/198 (52.53%), Query Frame = 1 

Query: 5   KTLLFTILLLILIRVQGQVFT---------HFFFVQQWPEALCKIQP-CDVDNV---PTK 64
           K LL ++ L+ L+ +   VF+          F+FVQQWP + C  Q  C   N       
Sbjct: 2   KILLASLCLISLLVILPSVFSASSSSEDFDFFYFVQQWPGSYCDTQKKCCYPNSGKPAAD 61

Query: 65  FTIHGLWPQ-NGQSYLIDCDSN--FEADKISHLTAQLSVEWPNLLDRHGF-EWFWTYEWN 124
           F IHGLWP     +Y  +CD++  F++  IS L   +   WP L    G  E FW +EW 
Sbjct: 62  FGIHGLWPNYKDGTYPSNCDASKPFDSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWE 121

Query: 125 KHGTCSQTQFSQAMYFQLALNMKKNVDLVSALEKGGIVPDDSLKLKKTVQAVLKAELGKE 184
           KHGTCS++   Q  YFQ ALN+K+  +L+ AL K GI PD      ++++  +K  +G  
Sbjct: 122 KHGTCSESVIDQHEYFQTALNLKQKTNLLGALTKAGINPDGKSYSLESIRDSIKESIGFT 181

Query: 185 PFLRCKNDSTQTARLVEM 186
           P++ C  D +  ++L ++
Sbjct: 182 PWVECNRDGSGNSQLYQV 199

[Close]
BLAST of Lsi11G010630 vs. Swiss-Prot
Match: RNS3_ARATH (Ribonuclease 3 OS=Arabidopsis thaliana GN=RNS3 PE=2 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 4.1e-24
Identity = 65/186 (34.95%), Postives = 94/186 (50.54%), Query Frame = 1 

Query: 11  ILLLILIRVQG--QVFTHFFFVQQWPEALCKIQPC----DVDNVPTKFTIHGLWPQ---N 70
           IL L  + VQ   Q F  F+FV QWP A C  +              F IHGLWP     
Sbjct: 7   ILALQQLYVQSFAQDFDFFYFVLQWPGAYCDSRHSCCYPQTGKPAADFGIHGLWPNYKTG 66

Query: 71  GQSYLIDCDSNFEADKISHLTAQLSVEWPNLL--DRHGFEWFWTYEWNKHGTCSQTQFSQ 130
           G     + DS F+  ++S L + L  EWP L      G + FWT+EW KHGTC++++  Q
Sbjct: 67  GWPQNCNPDSRFDDLRVSDLMSDLQREWPTLSCPSNDGMK-FWTHEWEKHGTCAESELDQ 126

Query: 131 AMYFQLALNMKKNVDLVSALEKGGIVPDDSLKLKKTVQAVLKAELGKEPFLRCKNDSTQT 186
             YF+  L +K+  +L+ AL   GI PDD     K ++  +K  +G  P + C  DS+  
Sbjct: 127 HDYFEAGLKLKQKANLLHALTNAGIKPDDKFYEMKDIENTIKQVVGFAPGIECNKDSSHN 186

[Close]
BLAST of Lsi11G010630 vs. Swiss-Prot
Match: RNLE_SOLLC (Extracellular ribonuclease LE OS=Solanum lycopersicum PE=1 SV=2)

HSP 1 Score: 109.4 bits (272), Expect = 4.5e-23
Identity = 63/193 (32.64%), Postives = 102/193 (52.85%), Query Frame = 1 

Query: 9   FTILLLILIRVQ-------GQVFTHFFFVQQWPEALCKI-QPC---DVDNVPTKFTIHGL 68
           F++ L++LI  Q        + F  F+FVQQWP + C   Q C           F IHGL
Sbjct: 7   FSLFLILLIITQCLSVLNAAKDFDFFYFVQQWPGSYCDTKQSCCYPTTGKPAADFGIHGL 66

Query: 69  WPQNGQ-SYLIDCDSN--FEADKISHLTAQLSVEWPNLLDRHGF-EWFWTYEWNKHGTCS 128
           WP N   +Y  +CD N  ++  +IS L + +   WP L    G    FW++EW KHGTC+
Sbjct: 67  WPNNNDGTYPSNCDPNSPYDQSQISDLISSMQQNWPTLACPSGSGSTFWSHEWEKHGTCA 126

Query: 129 QTQFS-QAMYFQLALNMKKNVDLVSALEKGGIVPDDSLKLKKTVQAVLKAELGKEPFLRC 186
           ++  + Q  YF+ AL++K  +DL+S L+   I PD        ++  +K+ +G  P+++C
Sbjct: 127 ESVLTNQHAYFKKALDLKNQIDLLSILQGADIHPDGESYDLVNIRNAIKSAIGYTPWIQC 186

[Close]
BLAST of Lsi11G010630 vs. Swiss-Prot
Match: RNS6_PYRPY (Ribonuclease S-6 OS=Pyrus pyrifolia PE=1 SV=1)

HSP 1 Score: 101.3 bits (251), Expect = 1.2e-20
Identity = 61/184 (33.15%), Postives = 99/184 (53.80%), Query Frame = 1 

Query: 13  LLILIRVQGQV-FTHFFFVQQWPEALCKIQPCDVDNVPTK-FTIHGLWPQN--GQSYLID 72
           L++LI     + + +F F QQ+  A+C   P    + P K FT+HGLWP N  G   +  
Sbjct: 16  LIVLISCSSTMGYNYFQFTQQYQPAVCNSNPTPCKDPPDKLFTVHGLWPSNDVGDDPIYC 75

Query: 73  CDSNFEADKISHLTAQLSVEWPNLLDRHGFEWFWTYEWNKHGTCSQ--TQFSQAMYFQLA 132
            +   ++ +I +LTAQL + WPN+LDR     FW  +WNKHG+C +  T   +  YF+  
Sbjct: 76  KNKTIKSQQIGNLTAQLIIIWPNVLDRTDHVGFWNRQWNKHGSCGKAPTIKDEMHYFKTV 135

Query: 133 LNM----KKNVDLVSALEKGGIVPDDSLKLK-KTVQAVLKAELGKEPFLRCKNDSTQTAR 186
           + M    K+NV  +  L +  I P+  ++ +   + A+      K+P L+C+ ++ QT  
Sbjct: 136 IKMYITQKQNVSEI--LSRAKIEPEGKIRRRDDIINAIRLGTKDKKPKLKCQKNN-QTTE 195

[Close]
BLAST of Lsi11G010630 vs. TrEMBL
Match: Q40115_LUFAE (Ribonuclease (RNase LC1) OS=Luffa aegyptiaca PE=2 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 1.1e-39
Identity = 83/189 (43.92%), Postives = 126/189 (66.67%), Query Frame = 1 

Query: 1   MMKKKTLLFTILLLILIRVQGQVFTHFFFVQQWPEALCKIQP--CDVDNVPTKFTIHGLW 60
           M K++ +L  +L ++   V+ Q F  F+ VQ WP A+C  Q   C V      FTIHG+W
Sbjct: 3   MAKREIVLVFVLTILFPMVKSQTFDSFWMVQHWPPAVCSFQQGRC-VGQGLRSFTIHGVW 62

Query: 61  PQNGQSYLIDCDS-NFEADKISHLTAQLSVEWPNLLDRHGFEWFWTYEWNKHGTCSQTQF 120
           PQ G + +I+C    F+  KISHL + L+V+WPN++  +  +WFW +EWNKHG CS ++F
Sbjct: 63  PQKGGTSVINCPGPTFDFTKISHLESTLNVDWPNVITGNN-KWFWGHEWNKHGICSVSKF 122

Query: 121 SQAMYFQLALNMKKNVDLVSALEKGGIVPDDSLKLKKTVQAVLKAELGKEPFLRCKNDST 180
            Q  YFQ+A+NM+ ++DL+SAL  GG+VP+   K ++ VQ+ ++A+LGKEP LRC+    
Sbjct: 123 DQQAYFQMAINMRNSIDLLSALRVGGVVPNGRSKARQRVQSAIRAQLGKEPVLRCRGTGR 182

Query: 181 QTARLVEML 187
           Q +RL+E++
Sbjct: 183 Q-SRLLEIV 188

[Close]
BLAST of Lsi11G010630 vs. TrEMBL
Match: Q40116_LUFAE (Ribonuclease (RNase LC2) OS=Luffa aegyptiaca PE=2 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 1.8e-39
Identity = 88/183 (48.09%), Postives = 115/183 (62.84%), Query Frame = 1 

Query: 1   MMKKKTLLFTILLLILIRVQG---QVFTHFFFVQQWPEALCKIQ--PCDVDNVPTKFTIH 60
           M  K  LL TI+LL+ I   G   Q F HFFFVQQWP   C+ Q  PC     P  F IH
Sbjct: 1   MATKVLLLATIVLLLTILFLGAESQTFDHFFFVQQWPPTTCQQQQKPC-FQPPPATFKIH 60

Query: 61  GLWPQNGQSYLIDCDSNFEADKISHLTAQLSVEWPNLLDRHGFEWFWTYEWNKHGTCSQT 120
           GLWPQ G + ++ C+ NF+  +IS L  QL V WP+++  +    FW +EWNKHG+CS++
Sbjct: 61  GLWPQKGPNSVVYCNKNFDRTQISSLENQLDVVWPDVVTGNN-TGFWEHEWNKHGSCSES 120

Query: 121 QFSQAMYFQLALNMKKNVDLVSALEKGGIVPDDSLKLKKTVQAVLKAELGKEPFLRCKND 179
           QF+Q +YFQ A+NM   V+L+ AL KGGI  D+  K  +T+Q VL A+ G +PFLRCK  
Sbjct: 121 QFNQTLYFQTAINMMNKVNLLKALGKGGITSDERTKSSQTMQKVLLAQFGNQPFLRCKKV 180

[Close]
BLAST of Lsi11G010630 vs. TrEMBL
Match: A0A0A0LC37_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G825050 PE=3 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 1.6e-35
Identity = 79/184 (42.93%), Postives = 114/184 (61.96%), Query Frame = 1 

Query: 7   LLFTILLLILIR-VQGQVFTHFFFVQQWPEALCKIQP--CDVDNVPTKFTIHGLWPQNGQ 66
           + F  +L IL   V+ Q F  F+FVQQWP A+C +Q   C V      FTIHGLWPQ G 
Sbjct: 10  VFFVFVLTILFPIVKSQTFDDFWFVQQWPPAVCTLQSGRC-VGRGTRSFTIHGLWPQKGG 69

Query: 67  SYLIDCDSN-FEADKISHLTAQLSVEWPNLLDRHGFEWFWTYEWNKHGTCSQTQFSQAMY 126
             + +C  N F+  KI+HL   L+V WPN++  +  ++FW +EWNKHG CS+++F +A Y
Sbjct: 70  RSVTNCTGNQFDFTKIAHLENDLNVVWPNVVTGNN-KFFWGHEWNKHGICSESKFDEAKY 129

Query: 127 FQLALNMKKNVDLVSALEKGGIVPDDSLKLKKTVQAVLKAELGKEPFLRCKNDSTQTARL 186
           FQ A+NM+  +DL+S L  GG+ P+ + K K+ V+  + +  GK+P LRCK  S     L
Sbjct: 130 FQTAINMRHGIDLLSVLRTGGVGPNGASKAKQRVETAISSHFGKDPILRCKKASNGQVLL 189

[Close]
BLAST of Lsi11G010630 vs. TrEMBL
Match: A0A0A0LHQ9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G825060 PE=3 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 1.1e-28
Identity = 72/185 (38.92%), Postives = 112/185 (60.54%), Query Frame = 1 

Query: 7   LLFTILLLILIR--VQGQVFTHFFFVQQWPEALC--KIQPCDVDNVPTKFTIHGLWPQNG 66
           LLF + L I +   ++ Q F  F+FVQQWP A+C  +   C V      FTIHG+WPQ G
Sbjct: 13  LLFVVTLSISLFPIMKSQQFDKFYFVQQWPPAVCDGRTGKC-VGKGMYYFTIHGVWPQKG 72

Query: 67  QSYLIDCD-SNFEADKISHLTAQLSVEWPNLLDRHGFEWFWTYEWNKHGTCSQTQFSQAM 126
              +I+C  + F+ +KIS L   L     ++++    ++ W++EWNKHG CS++++S   
Sbjct: 73  GKSVINCPGTQFDFNKISSLANTLHQIMKDVINADD-QFLWSHEWNKHGVCSESRYSMKQ 132

Query: 127 YFQLALNMKKNVDLVSALEKGGIVPDDSLKLKKTVQAVLKAELGKEPFLRCKNDSTQTAR 186
           YFQ+A+NMK  ++++SAL  GGI P++ LK K+ V+  +       P LRCK DS+  + 
Sbjct: 133 YFQMAINMKYKINVLSALRMGGITPNNHLKAKQRVEGAMFTAYNAYPLLRCKKDSSGQSL 192

[Close]
BLAST of Lsi11G010630 vs. TrEMBL
Match: Q8LP86_SOLPE (S25-RNase OS=Solanum peruvianum PE=2 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 3.6e-27
Identity = 74/188 (39.36%), Postives = 104/188 (55.32%), Query Frame = 1 

Query: 6   TLLFTILLLILIRVQGQVFTHFFFVQQWPEALCKIQPCDVDNVPTKFTIHGLWPQNGQSY 65
           ++LFT LL  L  V G  F +   V QWP   C  +PC     P  FTIHGLWP +    
Sbjct: 2   SVLFT-LLFSLAPVLGY-FEYLQLVLQWPTTFCHTKPCPTWPPPNNFTIHGLWPDSKGKM 61

Query: 66  LIDCDSNFEADKI--SHLTAQLSVEWPNLLDRHG----FEWFWTYEWNKHGTCSQTQFSQ 125
           L DC S  + D I  +H+  QL  +WPNL  R G    ++ FW YE+NKHGTCS  +++Q
Sbjct: 62  LNDCGSGDDYDDIPDAHMRKQLESDWPNLTSRAGEIKKYQEFWGYEFNKHGTCSMDRYNQ 121

Query: 126 AMYFQLALNMKKNVDLVSALEKGGIVPDDSLKLKKTVQAVLKAELGKEPFLRC-KNDSTQ 185
             YF+LAL +K   DL++ L   GI+P  +  + K V+  +KA     P L C    S +
Sbjct: 122 DQYFELALKLKNQFDLLNILRNHGIIPGKTCTV-KDVEDAIKAVTAHVPNLNCIVRSSQR 181

Query: 186 TARLVEML 187
           ++ ++E+L
Sbjct: 182 SSHIMELL 186

[Close]
BLAST of Lsi11G010630 vs. TAIR10
Match: AT2G02990.1 (AT2G02990.1 ribonuclease 1)

HSP 1 Score: 117.1 bits (292), Expect = 1.2e-26
Identity = 69/198 (34.85%), Postives = 104/198 (52.53%), Query Frame = 1 

Query: 5   KTLLFTILLLILIRVQGQVFT---------HFFFVQQWPEALCKIQP-CDVDNV---PTK 64
           K LL ++ L+ L+ +   VF+          F+FVQQWP + C  Q  C   N       
Sbjct: 2   KILLASLCLISLLVILPSVFSASSSSEDFDFFYFVQQWPGSYCDTQKKCCYPNSGKPAAD 61

Query: 65  FTIHGLWPQ-NGQSYLIDCDSN--FEADKISHLTAQLSVEWPNLLDRHGF-EWFWTYEWN 124
           F IHGLWP     +Y  +CD++  F++  IS L   +   WP L    G  E FW +EW 
Sbjct: 62  FGIHGLWPNYKDGTYPSNCDASKPFDSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWE 121

Query: 125 KHGTCSQTQFSQAMYFQLALNMKKNVDLVSALEKGGIVPDDSLKLKKTVQAVLKAELGKE 184
           KHGTCS++   Q  YFQ ALN+K+  +L+ AL K GI PD      ++++  +K  +G  
Sbjct: 122 KHGTCSESVIDQHEYFQTALNLKQKTNLLGALTKAGINPDGKSYSLESIRDSIKESIGFT 181

Query: 185 PFLRCKNDSTQTARLVEM 186
           P++ C  D +  ++L ++
Sbjct: 182 PWVECNRDGSGNSQLYQV 199

[Close]
BLAST of Lsi11G010630 vs. TAIR10
Match: AT1G26820.1 (AT1G26820.1 ribonuclease 3)

HSP 1 Score: 112.8 bits (281), Expect = 2.3e-25
Identity = 65/186 (34.95%), Postives = 94/186 (50.54%), Query Frame = 1 

Query: 11  ILLLILIRVQG--QVFTHFFFVQQWPEALCKIQPC----DVDNVPTKFTIHGLWPQ---N 70
           IL L  + VQ   Q F  F+FV QWP A C  +              F IHGLWP     
Sbjct: 7   ILALQQLYVQSFAQDFDFFYFVLQWPGAYCDSRHSCCYPQTGKPAADFGIHGLWPNYKTG 66

Query: 71  GQSYLIDCDSNFEADKISHLTAQLSVEWPNLL--DRHGFEWFWTYEWNKHGTCSQTQFSQ 130
           G     + DS F+  ++S L + L  EWP L      G + FWT+EW KHGTC++++  Q
Sbjct: 67  GWPQNCNPDSRFDDLRVSDLMSDLQREWPTLSCPSNDGMK-FWTHEWEKHGTCAESELDQ 126

Query: 131 AMYFQLALNMKKNVDLVSALEKGGIVPDDSLKLKKTVQAVLKAELGKEPFLRCKNDSTQT 186
             YF+  L +K+  +L+ AL   GI PDD     K ++  +K  +G  P + C  DS+  
Sbjct: 127 HDYFEAGLKLKQKANLLHALTNAGIKPDDKFYEMKDIENTIKQVVGFAPGIECNKDSSHN 186

[Close]
BLAST of Lsi11G010630 vs. TAIR10
Match: AT1G14220.1 (AT1G14220.1 Ribonuclease T2 family protein)

HSP 1 Score: 112.8 bits (281), Expect = 2.3e-25
Identity = 63/168 (37.50%), Postives = 84/168 (50.00%), Query Frame = 1 

Query: 24  FTHFFFVQQWPEALCKIQPCDVDNVPTK----FTIHGLWPQ-NGQSYLIDCD--SNFEAD 83
           F  F+FV QWP A C            K    F IHGLWP  NG S+  +CD  S F+  
Sbjct: 28  FDFFYFVLQWPGAYCDTSRACCYPTSGKPAADFGIHGLWPNYNGGSWPSNCDPDSQFDRS 87

Query: 84  KISHLTAQLSVEWPNLL--DRHGFEWFWTYEWNKHGTCSQTQFSQAMYFQLALNMKKNVD 143
           +IS L + L   WP L      GF  FW +EW KHGTCS++   Q  YF+ AL +K+  +
Sbjct: 88  QISDLVSSLKKNWPTLSCPSNEGFN-FWEHEWEKHGTCSESVMDQHEYFENALKLKQKAN 147

Query: 144 LVSALEKGGIVPDDSLKLKKTVQAVLKAELGKEPFLRCKNDSTQTARL 183
           L+  L+  GI PDD       +   +K  +G  P + C  D  + A+L
Sbjct: 148 LLQILKNSGINPDDGFYNLDKITNAIKDGIGFTPGIECNKDPERNAQL 194

[Close]
BLAST of Lsi11G010630 vs. TAIR10
Match: AT1G14210.1 (AT1G14210.1 Ribonuclease T2 family protein)

HSP 1 Score: 102.8 bits (255), Expect = 2.4e-22
Identity = 59/180 (32.78%), Postives = 83/180 (46.11%), Query Frame = 1 

Query: 24  FTHFFFVQQWPEALCKIQ----PCDVDNVPTKFTIHGLWPQ-NGQSYLIDCDSN--FEAD 83
           F  F++V  WP A+C  Q    P    N  + F IHGLWPQ N  ++   CD    F+  
Sbjct: 26  FNFFYWVNYWPGAICDSQKGCCPPTKGNTASDFIIHGLWPQFNNGTWPAFCDQTNLFDIS 85

Query: 84  KISHLTAQLSVEWPNLLDRHGFEW-----------FWTYEWNKHGTCSQTQFSQAMYFQL 143
           KIS L  Q+  +W         EW            W +EWNKHGTC Q+ F Q  YF+ 
Sbjct: 86  KISDLVCQMEKKWT--------EWGVWACPSNETNLWEHEWNKHGTCVQSIFDQHSYFRT 145

Query: 144 ALNMKKNVDLVSALEKGGIVPDDSLKLKKTVQAVLKAELGKEPFLRCKNDSTQTARLVEM 186
            L  K  V L++ L + GI P+D       ++  +K  +G  P + C  D     +L ++
Sbjct: 146 NLKFKHKVHLLNILIQKGIKPNDGFYSLDEIKNAIKCAIGFAPGIECNEDVKGNKQLFQI 197

[Close]
BLAST of Lsi11G010630 vs. TAIR10
Match: AT2G39780.1 (AT2G39780.1 ribonuclease 2)

HSP 1 Score: 78.6 bits (192), Expect = 4.8e-15
Identity = 58/178 (32.58%), Postives = 76/178 (42.70%), Query Frame = 1 

Query: 24  FTHFFFVQQWPEALC--------KIQPCDVDNVPTKFTIHGLWPQNGQSYLIDC--DSNF 83
           F +F    QWP   C        K   C   + PT+FTIHGLWP         C   S+F
Sbjct: 32  FDYFALSLQWPGTYCRGTRHCCSKNACCRGSDAPTQFTIHGLWPDYNDGSWPSCCYRSDF 91

Query: 84  EADKISHLTAQLSVEWPNL------LDRHGFEWFWTYEWNKHGTCSQTQF-SQAMYFQLA 143
           +  +IS L   L   WP+L          G   FW +EW KHGTCS   F  +  YF   
Sbjct: 92  KEKEISTLMDGLEKYWPSLSCGSPSSCNGGKGSFWGHEWEKHGTCSSPVFHDEYNYFLTT 151

Query: 144 LNMKKNVDLVSALEKGGIVPDDSLK--LKKTVQAVLKAELGKEPFLRCKNDSTQTARL 183
           LN+    ++   L + G V  +S K  L   V A+  A     P + CK D+    R+
Sbjct: 152 LNLYLKHNVTDVLYQAGYVASNSEKYPLGGIVTAIQNA-FHITPEVVCKRDAIDEIRI 208

[Close]
BLAST of Lsi11G010630 vs. NCBI nr
Match: gi|976231|dbj|BAA10891.1| (ribonuclease (RNase LC1) [Luffa aegyptiaca])

HSP 1 Score: 171.4 bits (433), Expect = 1.5e-39
Identity = 83/189 (43.92%), Postives = 126/189 (66.67%), Query Frame = 1 

Query: 1   MMKKKTLLFTILLLILIRVQGQVFTHFFFVQQWPEALCKIQP--CDVDNVPTKFTIHGLW 60
           M K++ +L  +L ++   V+ Q F  F+ VQ WP A+C  Q   C V      FTIHG+W
Sbjct: 3   MAKREIVLVFVLTILFPMVKSQTFDSFWMVQHWPPAVCSFQQGRC-VGQGLRSFTIHGVW 62

Query: 61  PQNGQSYLIDCDS-NFEADKISHLTAQLSVEWPNLLDRHGFEWFWTYEWNKHGTCSQTQF 120
           PQ G + +I+C    F+  KISHL + L+V+WPN++  +  +WFW +EWNKHG CS ++F
Sbjct: 63  PQKGGTSVINCPGPTFDFTKISHLESTLNVDWPNVITGNN-KWFWGHEWNKHGICSVSKF 122

Query: 121 SQAMYFQLALNMKKNVDLVSALEKGGIVPDDSLKLKKTVQAVLKAELGKEPFLRCKNDST 180
            Q  YFQ+A+NM+ ++DL+SAL  GG+VP+   K ++ VQ+ ++A+LGKEP LRC+    
Sbjct: 123 DQQAYFQMAINMRNSIDLLSALRVGGVVPNGRSKARQRVQSAIRAQLGKEPVLRCRGTGR 182

Query: 181 QTARLVEML 187
           Q +RL+E++
Sbjct: 183 Q-SRLLEIV 188

[Close]
BLAST of Lsi11G010630 vs. NCBI nr
Match: gi|976233|dbj|BAA10892.1| (ribonuclease (RNase LC2) [Luffa aegyptiaca])

HSP 1 Score: 170.6 bits (431), Expect = 2.6e-39
Identity = 88/183 (48.09%), Postives = 115/183 (62.84%), Query Frame = 1 

Query: 1   MMKKKTLLFTILLLILIRVQG---QVFTHFFFVQQWPEALCKIQ--PCDVDNVPTKFTIH 60
           M  K  LL TI+LL+ I   G   Q F HFFFVQQWP   C+ Q  PC     P  F IH
Sbjct: 1   MATKVLLLATIVLLLTILFLGAESQTFDHFFFVQQWPPTTCQQQQKPC-FQPPPATFKIH 60

Query: 61  GLWPQNGQSYLIDCDSNFEADKISHLTAQLSVEWPNLLDRHGFEWFWTYEWNKHGTCSQT 120
           GLWPQ G + ++ C+ NF+  +IS L  QL V WP+++  +    FW +EWNKHG+CS++
Sbjct: 61  GLWPQKGPNSVVYCNKNFDRTQISSLENQLDVVWPDVVTGNN-TGFWEHEWNKHGSCSES 120

Query: 121 QFSQAMYFQLALNMKKNVDLVSALEKGGIVPDDSLKLKKTVQAVLKAELGKEPFLRCKND 179
           QF+Q +YFQ A+NM   V+L+ AL KGGI  D+  K  +T+Q VL A+ G +PFLRCK  
Sbjct: 121 QFNQTLYFQTAINMMNKVNLLKALGKGGITSDERTKSSQTMQKVLLAQFGNQPFLRCKKV 180

[Close]
BLAST of Lsi11G010630 vs. NCBI nr
Match: gi|659085407|ref|XP_008443402.1| (PREDICTED: ribonuclease MC-like [Cucumis melo])

HSP 1 Score: 165.2 bits (417), Expect = 1.1e-37
Identity = 82/188 (43.62%), Postives = 119/188 (63.30%), Query Frame = 1 

Query: 3   KKKTLLFTILLLILIR-VQGQVFTHFFFVQQWPEALCKIQP--CDVDNVPTKFTIHGLWP 62
           K+  + F  +L IL   V+ Q F +F+FVQQWP A+C +Q   C V      FTIHGLWP
Sbjct: 5   KRDVVFFVFVLTILFPIVKSQTFDNFWFVQQWPPAICTLQSGRC-VGRGTRSFTIHGLWP 64

Query: 63  QNGQSYLIDCDSN-FEADKISHLTAQLSVEWPNLLDRHGFEWFWTYEWNKHGTCSQTQFS 122
           Q G   + +C  N F+  KI+HL + L+V WPN++  +  ++FW +EWNKHG CS+++F 
Sbjct: 65  QKGGRSVTNCSGNSFDFTKIAHLESDLNVVWPNVVTGNN-KFFWGHEWNKHGICSESKFD 124

Query: 123 QAMYFQLALNMKKNVDLVSALEKGGIVPDDSLKLKKTVQAVLKAELGKEPFLRCKNDSTQ 182
           QA YFQ A+NM+ ++DL+S L  GGIVP+ + K K+ V+  + +   K+P LRCK  S  
Sbjct: 125 QAKYFQTAINMRHSIDLLSVLRTGGIVPNGASKAKQRVETAISSHFAKDPILRCKTASNG 184

Query: 183 TARLVEML 187
              L E++
Sbjct: 185 QVLLTEIV 190

[Close]
BLAST of Lsi11G010630 vs. NCBI nr
Match: gi|449459530|ref|XP_004147499.1| (PREDICTED: ribonuclease MC-like [Cucumis sativus])

HSP 1 Score: 157.5 bits (397), Expect = 2.3e-35
Identity = 79/184 (42.93%), Postives = 114/184 (61.96%), Query Frame = 1 

Query: 7   LLFTILLLILIR-VQGQVFTHFFFVQQWPEALCKIQP--CDVDNVPTKFTIHGLWPQNGQ 66
           + F  +L IL   V+ Q F  F+FVQQWP A+C +Q   C V      FTIHGLWPQ G 
Sbjct: 10  VFFVFVLTILFPIVKSQTFDDFWFVQQWPPAVCTLQSGRC-VGRGTRSFTIHGLWPQKGG 69

Query: 67  SYLIDCDSN-FEADKISHLTAQLSVEWPNLLDRHGFEWFWTYEWNKHGTCSQTQFSQAMY 126
             + +C  N F+  KI+HL   L+V WPN++  +  ++FW +EWNKHG CS+++F +A Y
Sbjct: 70  RSVTNCTGNQFDFTKIAHLENDLNVVWPNVVTGNN-KFFWGHEWNKHGICSESKFDEAKY 129

Query: 127 FQLALNMKKNVDLVSALEKGGIVPDDSLKLKKTVQAVLKAELGKEPFLRCKNDSTQTARL 186
           FQ A+NM+  +DL+S L  GG+ P+ + K K+ V+  + +  GK+P LRCK  S     L
Sbjct: 130 FQTAINMRHGIDLLSVLRTGGVGPNGASKAKQRVETAISSHFGKDPILRCKKASNGQVLL 189

[Close]
BLAST of Lsi11G010630 vs. NCBI nr
Match: gi|5821838|pdb|1BK7|A (Chain A, Ribonuclease Mc1 From The Seeds Of Bitter Gourd)

HSP 1 Score: 149.8 bits (377), Expect = 4.8e-33
Identity = 75/168 (44.64%), Postives = 108/168 (64.29%), Query Frame = 1 

Query: 24  FTHFFFVQQWPEALCKIQP---CDVDNVPTKFTIHGLWPQNGQSYLIDCD-SNFEADKIS 83
           F  F+FVQQWP A+C  Q    C    + T FTIHGLWPQ   + L +C  S F+  KIS
Sbjct: 1   FDSFWFVQQWPPAVCSFQKSGSCPGSGLRT-FTIHGLWPQQSGTSLTNCPGSPFDITKIS 60

Query: 84  HLTAQLSVEWPNLLDRHGFEWFWTYEWNKHGTCSQTQFSQAMYFQLALNMKKNVDLVSAL 143
           HL +QL+  WPN+L R   + FW++EW KHGTCS++ F+QA YF+LA++M+ N D++ AL
Sbjct: 61  HLQSQLNTLWPNVL-RANNQQFWSHEWTKHGTCSESTFNQAAYFKLAVDMRNNYDIIGAL 120

Query: 144 EKGGIVPDDSLKLKKTVQAVLKAELGKEPFLRCKND-STQTARLVEML 187
                 P+   K ++ ++  LKA+ GK P LRC+ D  T+ + LV+++
Sbjct: 121 RPHAAGPNGRTKSRQAIKGFLKAKFGKFPGLRCRTDPQTKVSYLVQVV 166

The following BLAST results are available for this feature:
BLAST of Lsi11G010630 vs. Swiss-Prot
Match NameE-valueIdentityDescription
RNMC_MOMCH3.9e-3544.38Ribonuclease MC OS=Momordica charantia PE=1 SV=1[more]
RNS1_ARATH2.2e-2534.85Ribonuclease 1 OS=Arabidopsis thaliana GN=RNS1 PE=1 SV=1[more]
RNS3_ARATH4.1e-2434.95Ribonuclease 3 OS=Arabidopsis thaliana GN=RNS3 PE=2 SV=1[more]
RNLE_SOLLC4.5e-2332.64Extracellular ribonuclease LE OS=Solanum lycopersicum PE=1 SV=2[more]
RNS6_PYRPY1.2e-2033.15Ribonuclease S-6 OS=Pyrus pyrifolia PE=1 SV=1[more]
BLAST of Lsi11G010630 vs. TrEMBL
Match NameE-valueIdentityDescription
Q40115_LUFAE1.1e-3943.92Ribonuclease (RNase LC1) OS=Luffa aegyptiaca PE=2 SV=1[more]
Q40116_LUFAE1.8e-3948.09Ribonuclease (RNase LC2) OS=Luffa aegyptiaca PE=2 SV=1[more]
A0A0A0LC37_CUCSA1.6e-3542.93Uncharacterized protein OS=Cucumis sativus GN=Csa_3G825050 PE=3 SV=1[more]
A0A0A0LHQ9_CUCSA1.1e-2838.92Uncharacterized protein OS=Cucumis sativus GN=Csa_3G825060 PE=3 SV=1[more]
Q8LP86_SOLPE3.6e-2739.36S25-RNase OS=Solanum peruvianum PE=2 SV=1[more]
BLAST of Lsi11G010630 vs. TAIR10
Match NameE-valueIdentityDescription
AT2G02990.11.2e-2634.85 ribonuclease 1[more]
AT1G26820.12.3e-2534.95 ribonuclease 3[more]
AT1G14220.12.3e-2537.50 Ribonuclease T2 family protein[more]
AT1G14210.12.4e-2232.78 Ribonuclease T2 family protein[more]
AT2G39780.14.8e-1532.58 ribonuclease 2[more]
BLAST of Lsi11G010630 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|976231|dbj|BAA10891.1|1.5e-3943.92ribonuclease (RNase LC1) [Luffa aegyptiaca][more]
gi|976233|dbj|BAA10892.1|2.6e-3948.09ribonuclease (RNase LC2) [Luffa aegyptiaca][more]
gi|659085407|ref|XP_008443402.1|1.1e-3743.62PREDICTED: ribonuclease MC-like [Cucumis melo][more]
gi|449459530|ref|XP_004147499.1|2.3e-3542.93PREDICTED: ribonuclease MC-like [Cucumis sativus][more]
gi|5821838|pdb|1BK7|A4.8e-3344.64Chain A, Ribonuclease Mc1 From The Seeds Of Bitter Gourd[more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0033897ribonuclease T2 activity
GO:0003723RNA binding
Vocabulary: INTERPRO
TermDefinition
IPR018188RNase_T2_His_AS_1
IPR001568RNase_T2-like
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0033897 ribonuclease T2 activity
molecular_function GO:0003723 RNA binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi11G010630.1Lsi11G010630.1mRNA


InterPro
Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001568Ribonuclease T2-likeGENE3DG3DSA:3.90.730.10coord: 25..184
score: 6.6
IPR001568Ribonuclease T2-likePANTHERPTHR11240RIBONUCLEASE T2coord: 7..175
score: 3.9
IPR001568Ribonuclease T2-likePFAMPF00445Ribonuclease_T2coord: 27..178
score: 4.8
IPR001568Ribonuclease T2-likeunknownSSF55895Ribonuclease Rh-likecoord: 23..186
score: 7.98
IPR018188Ribonuclease T2, His active site 1PROSITEPS00530RNASE_T2_1coord: 52..59
scor

Synteny
The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Lsi11G010630Wax gourdlsiwgoB115
Lsi11G010630Wax gourdlsiwgoB128
Lsi11G010630Bottle gourd (USVL1VR-Ls)lsilsiB021
Lsi11G010630Cucumber (Gy14) v1cgylsiB146
Lsi11G010630Cucumber (Gy14) v1cgylsiB361
Lsi11G010630Cucurbita maxima (Rimu)cmalsiB092
Lsi11G010630Cucurbita maxima (Rimu)cmalsiB472
Lsi11G010630Cucurbita maxima (Rimu)cmalsiB562
Lsi11G010630Cucurbita maxima (Rimu)cmalsiB784
Lsi11G010630Cucurbita moschata (Rifu)cmolsiB089
Lsi11G010630Cucurbita moschata (Rifu)cmolsiB457
Lsi11G010630Cucurbita moschata (Rifu)cmolsiB545
Lsi11G010630Cucurbita pepo (Zucchini)cpelsiB199
Lsi11G010630Cucurbita pepo (Zucchini)cpelsiB533
Lsi11G010630Cucurbita pepo (Zucchini)cpelsiB605
Lsi11G010630Wild cucumber (PI 183967)cpilsiB097
Lsi11G010630Wild cucumber (PI 183967)cpilsiB435
Lsi11G010630Wild cucumber (PI 183967)cpilsiB507
Lsi11G010630Cucumber (Chinese Long) v2culsiB102
Lsi11G010630Cucumber (Chinese Long) v2culsiB495
Lsi11G010630Melon (DHL92) v3.5.1lsimeB097
Lsi11G010630Melon (DHL92) v3.5.1lsimeB120
Lsi11G010630Watermelon (Charleston Gray)lsiwcgB090
Lsi11G010630Watermelon (Charleston Gray)lsiwcgB097
Lsi11G010630Watermelon (97103) v1lsiwmB106
Lsi11G010630Cucumber (Gy14) v2cgyblsiB090
Lsi11G010630Cucumber (Gy14) v2cgyblsiB463
Lsi11G010630Melon (DHL92) v3.6.1lsimedB127
Lsi11G010630Melon (DHL92) v3.6.1lsimedB103
Lsi11G010630Silver-seed gourdcarlsiB186
Lsi11G010630Silver-seed gourdcarlsiB599
Lsi11G010630Silver-seed gourdcarlsiB809
Lsi11G010630Cucumber (Chinese Long) v3cuclsiB109
Lsi11G010630Cucumber (Chinese Long) v3cuclsiB530
Lsi11G010630Watermelon (97103) v2lsiwmbB086