Lsi11G010370 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi11G010370
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	chr11 : 17860552 .. 17861001 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAGCCCTTGAGGAGTTCGATCAGACTTTCATCCAACGCCCAGAGCACCGCCTCAAGCTTGCCATCTCCGAAGCTGACGGAATCCCCACCATCGACCTCTCTCCGATCTTCAACTCCCCCGGCTGCAATTTTCCCAACGATGTGGTCCGACAAATCGCCTCTGCTTCCAAGGAGTGGGGATTCTTTCTGGTGGTCAATCACGGCGTCCCTCCCGAGAAGCAGCGCCGGATAGAGGTCGCTGCCCGAGAGTTTTTCGGGCAGAGTTTGGAAGAGAAGAGGAAAGTGAGGAGAAACGAAGGGGTGGTGACTGGCTATTTTGATACGGAGCTGACCAAGAACGTAAGAGATTGGAAGGAGGTGTTTGATTTCATGGTGGCGAATCATCCTGATGACGACAAGTTGATTCATGTAGGTCTCACAGTCACTGTCCAATTGTTTAAACTTTAG

mRNA sequence

ATGGGAGCCCTTGAGGAGTTCGATCAGACTTTCATCCAACGCCCAGAGCACCGCCTCAAGCTTGCCATCTCCGAAGCTGACGGAATCCCCACCATCGACCTCTCTCCGATCTTCAACTCCCCCGGCTGCAATTTTCCCAACGATGTGGTCCGACAAATCGCCTCTGCTTCCAAGGAGTGGGGATTCTTTCTGGTGGTCAATCACGGCGTCCCTCCCGAGAAGCAGCGCCGGATAGAGGTCGCTGCCCGAGAGTTTTTCGGGCAGAGTTTGGAAGAGAAGAGGAAAGTGAGGAGAAACGAAGGGGTGGTGACTGGCTATTTTGATACGGAGCTGACCAAGAACGTAAGAGATTGGAAGGAGGTGTTTGATTTCATGGTGGCGAATCATCCTGATGACGACAAGTTGATTCATGTAGGTCTCACAGTCACTGTCCAATTGTTTAAACTTTAG

Coding sequence (CDS)

ATGGGAGCCCTTGAGGAGTTCGATCAGACTTTCATCCAACGCCCAGAGCACCGCCTCAAGCTTGCCATCTCCGAAGCTGACGGAATCCCCACCATCGACCTCTCTCCGATCTTCAACTCCCCCGGCTGCAATTTTCCCAACGATGTGGTCCGACAAATCGCCTCTGCTTCCAAGGAGTGGGGATTCTTTCTGGTGGTCAATCACGGCGTCCCTCCCGAGAAGCAGCGCCGGATAGAGGTCGCTGCCCGAGAGTTTTTCGGGCAGAGTTTGGAAGAGAAGAGGAAAGTGAGGAGAAACGAAGGGGTGGTGACTGGCTATTTTGATACGGAGCTGACCAAGAACGTAAGAGATTGGAAGGAGGTGTTTGATTTCATGGTGGCGAATCATCCTGATGACGACAAGTTGATTCATGTAGGTCTCACAGTCACTGTCCAATTGTTTAAACTTTAG

Protein sequence

MGALEEFDQTFIQRPEHRLKLAISEADGIPTIDLSPIFNSPGCNFPNDVVRQIASASKEWGFFLVVNHGVPPEKQRRIEVAAREFFGQSLEEKRKVRRNEGVVTGYFDTELTKNVRDWKEVFDFMVANHPDDDKLIHVGLTVTVQLFKL

Homology

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BLAST of Lsi11G010370 vs. Swiss-Prot
Match: FLS_MATIN (Flavonol synthase/flavanone 3-hydroxylase (Fragment) OS=Matthiola incana PE=2 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 6.4e-09
Identity = 32/78 (41.03%), Postives = 41/78 (52.56%), Query Frame = 1


Query: 29  IPTIDLSPIFNSPGCNFPNDVVRQIASASKEWGFFLVVNHGVPPEKQRRIEVAAREFFGQ 88
           +P +DLS       C     V R +  AS++WG F VVNHG+P E  +R++   REFF  
Sbjct: 2   VPVVDLS-------CPDEELVARTVVKASEDWGVFQVVNHGIPTELIQRLQKVGREFFEL 61

Query: 89  SLEEKRKVRRNEGVVTGY 107
              EKR   R  G V GY
Sbjct: 62  PEAEKRSCAREAGSVEGY 72

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BLAST of Lsi11G010370 vs. Swiss-Prot
Match: F6H1_ARATH (Feruloyl CoA ortho-hydroxylase 1 OS=Arabidopsis thaliana GN=F6'H1 PE=1 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 6.4e-09
Identity = 38/137 (27.74%), Postives = 65/137 (47.45%), Query Frame = 1


Query: 4   LEEFDQTFIQRPEHRL--KLAISEADGIPTIDLSPIFNSPGCNFPNDVVRQIASASKEWG 63
           ++   + +IQ  E RL  K      + IP ID+S    +P     + V   +  A+++WG
Sbjct: 35  IKALPEQYIQPLEERLINKFVNETDEAIPVIDMS----NPD---EDRVAEAVCDAAEKWG 94

Query: 64  FFLVVNHGVPPEKQRRIEVAAREFFGQSLEEKRKVRRNEGVVT----GYFDTELTKNVRD 123
           FF V+NHGVP E    ++ A  +FF   +EEKRK  +   + T    G   + L +   +
Sbjct: 95  FFQVINHGVPLEVLDDVKAATHKFFNLPVEEKRKFTKENSLSTTVRFGTSFSPLAEQALE 154

Query: 124 WKEVFDFMVANHPDDDK 135
           WK+       +  + ++
Sbjct: 155 WKDYLSLFFVSEAEAEQ 164

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BLAST of Lsi11G010370 vs. Swiss-Prot
Match: F6H2_ARATH (Feruloyl CoA ortho-hydroxylase 2 OS=Arabidopsis thaliana GN=F6'H2 PE=1 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 8.3e-09
Identity = 34/113 (30.09%), Postives = 54/113 (47.79%), Query Frame = 1


Query: 27  DGIPTIDLSPIFNSPGCNFPNDVVRQIASASKEWGFFLVVNHGVPPEKQRRIEVAAREFF 86
           + IP ID+S +           V + +  A++EWGFF V+NHGV  E    ++ A   FF
Sbjct: 60  ESIPVIDISNLDEK-------SVSKAVCDAAEEWGFFQVINHGVSMEVLENMKTATHRFF 119

Query: 87  GQSLEEKRKVRRNEGVVTGY-FDTELTKNVR---DWKEVFDFMVANHPDDDKL 136
           G  +EEKRK  R + + T   F T  + +     +WK+       +  +  +L
Sbjct: 120 GLPVEEKRKFSREKSLSTNVRFGTSFSPHAEKALEWKDYLSLFFVSEAEASQL 165

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BLAST of Lsi11G010370 vs. Swiss-Prot
Match: GAOX1_ARATH (Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana GN=GA20OX1 PE=2 SV=2)

HSP 1 Score: 60.8 bits (146), Expect = 1.4e-08
Identity = 41/121 (33.88%), Postives = 54/121 (44.63%), Query Frame = 1


Query: 11  FIQRPEHRLKLAISEADGIPTIDLSPIFNSPGCNFPNDVVRQIASASKEWGFFLVVNHGV 70
           FI   + +  + + E D +P IDL  + + P      D  R I+ A K+ GFFLVVNHG+
Sbjct: 44  FIWPDDEKPSINVLELD-VPLIDLQNLLSDPSSTL--DASRLISEACKKHGFFLVVNHGI 103

Query: 71  PPEKQRRIEVAAREFFGQSLEEKRKVRRNEGVVTGY-------FDTELTKNVRDWKEVFD 125
             E           FF   L EK++V R  G   GY       F T+L      WKE   
Sbjct: 104 SEELISDAHEYTSRFFDMPLSEKQRVLRKSGESVGYASSFTGRFSTKL-----PWKETLS 156

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BLAST of Lsi11G010370 vs. Swiss-Prot
Match: LDOX_ARATH (Leucoanthocyanidin dioxygenase OS=Arabidopsis thaliana GN=LDOX PE=1 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 2.4e-08
Identity = 35/124 (28.23%), Postives = 61/124 (49.19%), Query Frame = 1


Query: 4   LEEFDQTFIQRPEHRLKLAISEADGIPTIDLSPIFNSPGCNFPNDVVRQIASASKEWGFF 63
           LE  +  F++  +        +   +PTIDL  I  S       + + ++  AS +WG  
Sbjct: 29  LESINDVFLEEKKE-------DGPQVPTIDLKNI-ESDDEKIRENCIEELKKASLDWGVM 88

Query: 64  LVVNHGVPPEKQRRIEVAAREFFGQSLEEKRKVRRNE--GVVTGYFDTELTKNVR---DW 123
            ++NHG+P +   R++ A  EFF  S+EEK K   ++  G + GY  ++L  N     +W
Sbjct: 89  HLINHGIPADLMERVKKAGEEFFSLSVEEKEKYANDQATGKIQGY-GSKLANNASGQLEW 143

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BLAST of Lsi11G010370 vs. TrEMBL
Match: A0A0A0K6P5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G074950 PE=3 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 3.8e-53
Identity = 108/146 (73.97%), Postives = 122/146 (83.56%), Query Frame = 1


Query: 1   MGALEEFDQTFIQRPEHRLKLAISEADGIPTIDLSPIFN-SP--GCNFPNDVVRQIASAS 60
           MG L+EFDQ FIQ P+HR KLA+S+ADGIPTIDLSPIFN SP  G  FP+D+V+QIASA 
Sbjct: 1   MGHLQEFDQVFIQAPDHRPKLAVSQADGIPTIDLSPIFNDSPPAGSEFPHDLVQQIASAC 60

Query: 61  KEWGFFLVVNHGVPPEKQRRIEVAAREFFGQSLEEKRKVRRNEGVVTGYFDTELTKNVRD 120
            EWGFFLVVNHGVPPEK+RR+E AAREFFGQSLEEKRKVRRNEGV TGYFD ELTKNVRD
Sbjct: 61  TEWGFFLVVNHGVPPEKRRRMEAAAREFFGQSLEEKRKVRRNEGVATGYFDMELTKNVRD 120

Query: 121 WKEVFDFMV--------ANHPDDDKL 136
           WKEVFDF+V        ++ PD+ +L
Sbjct: 121 WKEVFDFVVEDPTLIPASSDPDETEL 146

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BLAST of Lsi11G010370 vs. TrEMBL
Match: K7ZTB8_CUCSA (Gibberellin 20-oxidase (Fragment) OS=Cucumis sativus GN=ga20ox2 PE=2 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 3.8e-53
Identity = 108/146 (73.97%), Postives = 122/146 (83.56%), Query Frame = 1


Query: 1   MGALEEFDQTFIQRPEHRLKLAISEADGIPTIDLSPIFN-SP--GCNFPNDVVRQIASAS 60
           MG L+EFDQ FIQ P+HR KLA+S+ADGIPTIDLSPIFN SP  G  FP+D+V+QIASA 
Sbjct: 1   MGHLQEFDQVFIQAPDHRPKLAVSQADGIPTIDLSPIFNDSPPAGSEFPHDLVQQIASAC 60

Query: 61  KEWGFFLVVNHGVPPEKQRRIEVAAREFFGQSLEEKRKVRRNEGVVTGYFDTELTKNVRD 120
            EWGFFLVVNHGVPPEK+RR+E AAREFFGQSLEEKRKVRRNEGV TGYFD ELTKNVRD
Sbjct: 61  TEWGFFLVVNHGVPPEKRRRMEAAAREFFGQSLEEKRKVRRNEGVATGYFDMELTKNVRD 120

Query: 121 WKEVFDFMV--------ANHPDDDKL 136
           WKEVFDF+V        ++ PD+ +L
Sbjct: 121 WKEVFDFVVEDPTLIPASSDPDETEL 146

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BLAST of Lsi11G010370 vs. TrEMBL
Match: A0A0A0K834_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G074940 PE=4 SV=1)

HSP 1 Score: 213.0 bits (541), Expect = 2.5e-52
Identity = 107/145 (73.79%), Postives = 117/145 (80.69%), Query Frame = 1


Query: 1   MGALEEFDQTFIQRPEHRLKLAISEADGIPTIDLSPIFNS--PGCNFPNDVVRQIASASK 60
           MG L EFDQ FIQ PEHR KLA+S AD IPTIDLSPIFN   PG + P+D+V QIASA  
Sbjct: 1   MGHLHEFDQAFIQAPEHRPKLALSHADRIPTIDLSPIFNHSLPGSDVPHDLVHQIASACT 60

Query: 61  EWGFFLVVNHGVPPEKQRRIEVAAREFFGQSLEEKRKVRRNEGVVTGYFDTELTKNVRDW 120
           EWGFFLVVNHGVPPEK+ RIE AAREFFGQSLEEKRKVRR++G+VTGYFDTELTKNVRDW
Sbjct: 61  EWGFFLVVNHGVPPEKRHRIEAAAREFFGQSLEEKRKVRRSQGLVTGYFDTELTKNVRDW 120

Query: 121 KEVFDFMVAN--------HPDDDKL 136
           KEVFDFMV +          DDD+L
Sbjct: 121 KEVFDFMVEDPTIVPASPESDDDEL 145

[Close]

BLAST of Lsi11G010370 vs. TrEMBL
Match: A0A0A0K3A9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G074960 PE=3 SV=1)

HSP 1 Score: 203.4 bits (516), Expect = 2.0e-49
Identity = 101/127 (79.53%), Postives = 111/127 (87.40%), Query Frame = 1


Query: 1   MGALEEFDQTFIQRPEHRLKLAISEADGIPTIDLSPIFNS-PGCNFPNDVVRQIASASKE 60
           MG LE+F    IQ PEHR KLA+S ADGIPTIDLSPIFNS PG +FP+D+V QIASA  E
Sbjct: 1   MGDLEDF----IQAPEHRPKLALSHADGIPTIDLSPIFNSLPGSDFPHDLVHQIASACTE 60

Query: 61  WGFFLVVNHGVPPEKQRRIEVAAREFFGQSLEEKRKVRRNEGVVTGYFDTELTKNVRDWK 120
           WGFFLVVNHGVPPEK+ RIE AAREFFGQSLEEKRKVRR+EG+VTGYFD+ELTKNVRDWK
Sbjct: 61  WGFFLVVNHGVPPEKRHRIEAAAREFFGQSLEEKRKVRRSEGLVTGYFDSELTKNVRDWK 120

Query: 121 EVFDFMV 127
           EVFD +V
Sbjct: 121 EVFDLVV 123

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BLAST of Lsi11G010370 vs. TrEMBL
Match: M5VUD9_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa023403mg PE=3 SV=1)

HSP 1 Score: 154.8 bits (390), Expect = 8.0e-35
Identity = 76/124 (61.29%), Postives = 94/124 (75.81%), Query Frame = 1


Query: 6   EFDQTFIQRPEHRLKLAISEADGIPTIDLSPIFNSPGCNFPN---DVVRQIASASKEWGF 65
           E D TFIQ PEHR KL+++EA+GIP IDLSP+ +S   + P     VVR+I +A KEWGF
Sbjct: 3   EVDPTFIQDPEHRPKLSVTEAEGIPLIDLSPLNSSDNISDPKAIEGVVREIGNACKEWGF 62

Query: 66  FLVVNHGVPPEKQRRIEVAAREFFGQSLEEKRKVRRNEGVVTGYFDTELTKNVRDWKEVF 125
           F V+NHGV  +K ++IE AAR+FF Q LEEKRK+ R+E  + GY+DTE TKNVRDWKEVF
Sbjct: 63  FQVINHGVLLDKHKKIEDAARKFFAQPLEEKRKITRDENSLFGYYDTEHTKNVRDWKEVF 122

Query: 126 DFMV 127
           DF V
Sbjct: 123 DFAV 126

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BLAST of Lsi11G010370 vs. TAIR10
Match: AT3G19000.1 (AT3G19000.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 117.9 bits (294), Expect = 5.5e-27
Identity = 60/127 (47.24%), Postives = 80/127 (62.99%), Query Frame = 1


Query: 6   EFDQTFIQRPEHRLKLAISEA------DGIPTIDLSPIFNSPGCNFPNDVVRQIASASKE 65
           E D+ FIQ PEHR    ++ +      D IPTIDLS + ++   +    + ++IA A K 
Sbjct: 3   ELDEAFIQAPEHRPNTHLTNSGDFIFSDEIPTIDLSSLEDTH--HDKTAIAKEIAEACKR 62

Query: 66  WGFFLVVNHGVPPEKQRRIEVAAREFFGQSLEEKRKVRRNEGVVTGYFDTELTKNVRDWK 125
           WGFF V+NHG+P   + R+E  A EFF  + EEKRKV+R+E    GY D E TKNVRDWK
Sbjct: 63  WGFFQVINHGLPSALRHRVEKTAAEFFNLTTEEKRKVKRDEVNPMGYHDEEHTKNVRDWK 122

Query: 126 EVFDFMV 127
           E+FDF +
Sbjct: 123 EIFDFFL 127

[Close]

BLAST of Lsi11G010370 vs. TAIR10
Match: AT3G19010.1 (AT3G19010.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 105.9 bits (263), Expect = 2.2e-23
Identity = 53/122 (43.44%), Postives = 77/122 (63.11%), Query Frame = 1


Query: 4   LEEFDQTFIQRPEHRL--KLAISEADGIPTIDLSPIFNSPGCNFPNDVVRQIASASKEWG 63
           +E+ D T+IQ PEHR       ++ + IP IDLS + +        +V+ +I  A ++WG
Sbjct: 1   MEDLDPTYIQAPEHRSDSNFIPNQPEEIPVIDLSRLDDPEDVQ---NVISEIGDACEKWG 60

Query: 64  FFLVVNHGVPPEKQRRIEVAAREFFGQSLEEKRKVRRNEGVVTGYFDTELTKNVRDWKEV 123
           FF V+NHGVP + ++R+E   + FF   +EEK KV+R++    GY D E TKNV+DWKEV
Sbjct: 61  FFQVLNHGVPSDARQRVEKTVKMFFDLPMEEKIKVKRDDVNPVGYHDGEHTKNVKDWKEV 119

[Close]

BLAST of Lsi11G010370 vs. TAIR10
Match: AT3G21420.1 (AT3G21420.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 63.9 bits (154), Expect = 9.4e-11
Identity = 38/104 (36.54%), Postives = 54/104 (51.92%), Query Frame = 1


Query: 29  IPTIDLSPIFNSPGCNFPNDVVRQIASASKEWGFFLVVNHGVPPEKQRRIEVAAREFFGQ 88
           IP IDLS +      +F  ++++ ++ A ++WGFF V+NHG+  E    IE  A EFF  
Sbjct: 55  IPVIDLSKLSKPDNDDFFFEILK-LSQACEDWGFFQVINHGIEVEVVEDIEEVASEFFDM 114

Query: 89  SLEEKRKVRRNEGVVTGYFDTELTKNVR--DWKEVFDFMVANHP 131
            LEEK+K     G V GY    +    +  DW  +F   V  HP
Sbjct: 115 PLEEKKKYPMEPGTVQGYGQAFIFSEDQKLDWCNMFALGV--HP 155

[Close]

BLAST of Lsi11G010370 vs. TAIR10
Match: AT2G38240.1 (AT2G38240.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 62.8 bits (151), Expect = 2.1e-10
Identity = 35/96 (36.46%), Postives = 51/96 (53.12%), Query Frame = 1


Query: 29  IPTIDLSPIFNSPGCNFPNDVVRQIASASKEWGFFLVVNHGVPPEKQRRIEVAAREFFGQ 88
           IP +D++ ++  P      + +R + SA +EWGFF +VNHGV      R+  A REFF  
Sbjct: 48  IPVLDMNDVWGKP------EGLRLVRSACEEWGFFQMVNHGVTHSLMERVRGAWREFFEL 107

Query: 89  SLEEKRKVRRNEGVVTGYFD-TELTKNVR-DWKEVF 123
            LEEKRK   +     GY     + K+ + DW + F
Sbjct: 108 PLEEKRKYANSPDTYEGYGSRLGVVKDAKLDWSDYF 137

[Close]

BLAST of Lsi11G010370 vs. TAIR10
Match: AT3G13610.1 (AT3G13610.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 62.0 bits (149), Expect = 3.6e-10
Identity = 38/137 (27.74%), Postives = 65/137 (47.45%), Query Frame = 1


Query: 4   LEEFDQTFIQRPEHRL--KLAISEADGIPTIDLSPIFNSPGCNFPNDVVRQIASASKEWG 63
           ++   + +IQ  E RL  K      + IP ID+S    +P     + V   +  A+++WG
Sbjct: 35  IKALPEQYIQPLEERLINKFVNETDEAIPVIDMS----NPD---EDRVAEAVCDAAEKWG 94

Query: 64  FFLVVNHGVPPEKQRRIEVAAREFFGQSLEEKRKVRRNEGVVT----GYFDTELTKNVRD 123
           FF V+NHGVP E    ++ A  +FF   +EEKRK  +   + T    G   + L +   +
Sbjct: 95  FFQVINHGVPLEVLDDVKAATHKFFNLPVEEKRKFTKENSLSTTVRFGTSFSPLAEQALE 154

Query: 124 WKEVFDFMVANHPDDDK 135
           WK+       +  + ++
Sbjct: 155 WKDYLSLFFVSEAEAEQ 164

[Close]

BLAST of Lsi11G010370 vs. NCBI nr
Match: gi|659109825|ref|XP_008454901.1| (PREDICTED: hyoscyamine 6-dioxygenase-like isoform X2 [Cucumis melo])

HSP 1 Score: 220.7 bits (561), Expect = 1.7e-54
Identity = 110/144 (76.39%), Postives = 122/144 (84.72%), Query Frame = 1


Query: 1   MGALEEFDQTFIQRPEHRLKLAISEADGIPTIDLSPIFNS-PGCNFPNDVVRQIASASKE 60
           MG LE+FD  FIQ PEHR KLA+S+ADGIPTIDLSPIFNS PG +FP+ +V+QIASA  E
Sbjct: 1   MGDLEDFDHAFIQAPEHRPKLALSDADGIPTIDLSPIFNSVPGTDFPHHLVQQIASACTE 60

Query: 61  WGFFLVVNHGVPPEKQRRIEVAAREFFGQSLEEKRKVRRNEGVVTGYFDTELTKNVRDWK 120
           WGFFLVVNHGVPPEK+RRIE AAREFFGQSLEEKRKVRRNE VVTGYFDTELTKNVRDWK
Sbjct: 61  WGFFLVVNHGVPPEKRRRIEAAAREFFGQSLEEKRKVRRNEWVVTGYFDTELTKNVRDWK 120

Query: 121 EVFDFMV--------ANHPDDDKL 136
           EVFDF+V        ++ PDD +L
Sbjct: 121 EVFDFVVEDPTVIPASSDPDDTEL 144

[Close]

BLAST of Lsi11G010370 vs. NCBI nr
Match: gi|659109823|ref|XP_008454900.1| (PREDICTED: hyoscyamine 6-dioxygenase-like isoform X1 [Cucumis melo])

HSP 1 Score: 220.7 bits (561), Expect = 1.7e-54
Identity = 110/144 (76.39%), Postives = 122/144 (84.72%), Query Frame = 1


Query: 1   MGALEEFDQTFIQRPEHRLKLAISEADGIPTIDLSPIFNS-PGCNFPNDVVRQIASASKE 60
           MG LE+FD  FIQ PEHR KLA+S+ADGIPTIDLSPIFNS PG +FP+ +V+QIASA  E
Sbjct: 1   MGDLEDFDHAFIQAPEHRPKLALSDADGIPTIDLSPIFNSVPGTDFPHHLVQQIASACTE 60

Query: 61  WGFFLVVNHGVPPEKQRRIEVAAREFFGQSLEEKRKVRRNEGVVTGYFDTELTKNVRDWK 120
           WGFFLVVNHGVPPEK+RRIE AAREFFGQSLEEKRKVRRNE VVTGYFDTELTKNVRDWK
Sbjct: 61  WGFFLVVNHGVPPEKRRRIEAAAREFFGQSLEEKRKVRRNEWVVTGYFDTELTKNVRDWK 120

Query: 121 EVFDFMV--------ANHPDDDKL 136
           EVFDF+V        ++ PDD +L
Sbjct: 121 EVFDFVVEDPTVIPASSDPDDTEL 144

[Close]

BLAST of Lsi11G010370 vs. NCBI nr
Match: gi|659109829|ref|XP_008454903.1| (PREDICTED: LOW QUALITY PROTEIN: gibberellin 20 oxidase 1-like [Cucumis melo])

HSP 1 Score: 220.3 bits (560), Expect = 2.2e-54
Identity = 108/144 (75.00%), Postives = 123/144 (85.42%), Query Frame = 1


Query: 1   MGALEEFDQTFIQRPEHRLKLAISEADGIPTIDLSPIFNS-PGCNFPNDVVRQIASASKE 60
           MG LEEFDQ F+Q PEHR KLA+S+AD IPTIDLSPIFNS PG +FP+D+V+QIA+A  E
Sbjct: 1   MGDLEEFDQAFVQSPEHRPKLALSDADEIPTIDLSPIFNSVPGSDFPDDLVQQIAAACTE 60

Query: 61  WGFFLVVNHGVPPEKQRRIEVAAREFFGQSLEEKRKVRRNEGVVTGYFDTELTKNVRDWK 120
           WGFFLVVNHGVPPEK+ RIE AAREFFGQSLEEKRKVRR+EG+VTGYFDTELTKNVRDWK
Sbjct: 61  WGFFLVVNHGVPPEKRHRIEAAAREFFGQSLEEKRKVRRSEGMVTGYFDTELTKNVRDWK 120

Query: 121 EVFDFMVAN--------HPDDDKL 136
           EVFD +VA+         PDD++L
Sbjct: 121 EVFDLVVADPTIVPASPEPDDEEL 144

[Close]

BLAST of Lsi11G010370 vs. NCBI nr
Match: gi|449438440|ref|XP_004136996.1| (PREDICTED: hyoscyamine 6-dioxygenase-like isoform X2 [Cucumis sativus])

HSP 1 Score: 215.7 bits (548), Expect = 5.5e-53
Identity = 108/146 (73.97%), Postives = 122/146 (83.56%), Query Frame = 1


Query: 1   MGALEEFDQTFIQRPEHRLKLAISEADGIPTIDLSPIFN-SP--GCNFPNDVVRQIASAS 60
           MG L+EFDQ FIQ P+HR KLA+S+ADGIPTIDLSPIFN SP  G  FP+D+V+QIASA 
Sbjct: 1   MGHLQEFDQVFIQAPDHRPKLAVSQADGIPTIDLSPIFNDSPPAGSEFPHDLVQQIASAC 60

Query: 61  KEWGFFLVVNHGVPPEKQRRIEVAAREFFGQSLEEKRKVRRNEGVVTGYFDTELTKNVRD 120
            EWGFFLVVNHGVPPEK+RR+E AAREFFGQSLEEKRKVRRNEGV TGYFD ELTKNVRD
Sbjct: 61  TEWGFFLVVNHGVPPEKRRRMEAAAREFFGQSLEEKRKVRRNEGVATGYFDMELTKNVRD 120

Query: 121 WKEVFDFMV--------ANHPDDDKL 136
           WKEVFDF+V        ++ PD+ +L
Sbjct: 121 WKEVFDFVVEDPTLIPASSDPDETEL 146

[Close]

BLAST of Lsi11G010370 vs. NCBI nr
Match: gi|413915332|emb|CBX88044.1| (gibberellin 20-oxidase, partial [Cucumis sativus])

HSP 1 Score: 215.7 bits (548), Expect = 5.5e-53
Identity = 108/146 (73.97%), Postives = 122/146 (83.56%), Query Frame = 1


Query: 1   MGALEEFDQTFIQRPEHRLKLAISEADGIPTIDLSPIFN-SP--GCNFPNDVVRQIASAS 60
           MG L+EFDQ FIQ P+HR KLA+S+ADGIPTIDLSPIFN SP  G  FP+D+V+QIASA 
Sbjct: 1   MGHLQEFDQVFIQAPDHRPKLAVSQADGIPTIDLSPIFNDSPPAGSEFPHDLVQQIASAC 60

Query: 61  KEWGFFLVVNHGVPPEKQRRIEVAAREFFGQSLEEKRKVRRNEGVVTGYFDTELTKNVRD 120
            EWGFFLVVNHGVPPEK+RR+E AAREFFGQSLEEKRKVRRNEGV TGYFD ELTKNVRD
Sbjct: 61  TEWGFFLVVNHGVPPEKRRRMEAAAREFFGQSLEEKRKVRRNEGVATGYFDMELTKNVRD 120

Query: 121 WKEVFDFMV--------ANHPDDDKL 136
           WKEVFDF+V        ++ PD+ +L
Sbjct: 121 WKEVFDFVVEDPTLIPASSDPDETEL 146

The following BLAST results are available for this feature:

BLAST of Lsi11G010370 vs. Swiss-Prot


Match Name	E-value	Identity	Description
FLS_MATIN	6.4e-09	41.03	Flavonol synthase/flavanone 3-hydroxylase (Fragment) OS=Matthiola incana PE=2 SV...	[more]
F6H1_ARATH	6.4e-09	27.74	Feruloyl CoA ortho-hydroxylase 1 OS=Arabidopsis thaliana GN=F6'H1 PE=1 SV=1	[more]
F6H2_ARATH	8.3e-09	30.09	Feruloyl CoA ortho-hydroxylase 2 OS=Arabidopsis thaliana GN=F6'H2 PE=1 SV=1	[more]
GAOX1_ARATH	1.4e-08	33.88	Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana GN=GA20OX1 PE=2 SV=2	[more]
LDOX_ARATH	2.4e-08	28.23	Leucoanthocyanidin dioxygenase OS=Arabidopsis thaliana GN=LDOX PE=1 SV=1	[more]

BLAST of Lsi11G010370 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K6P5_CUCSA	3.8e-53	73.97	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G074950 PE=3 SV=1	[more]
K7ZTB8_CUCSA	3.8e-53	73.97	Gibberellin 20-oxidase (Fragment) OS=Cucumis sativus GN=ga20ox2 PE=2 SV=1	[more]
A0A0A0K834_CUCSA	2.5e-52	73.79	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G074940 PE=4 SV=1	[more]
A0A0A0K3A9_CUCSA	2.0e-49	79.53	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G074960 PE=3 SV=1	[more]
M5VUD9_PRUPE	8.0e-35	61.29	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa023403mg PE=3 SV=1	[more]

BLAST of Lsi11G010370 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G19000.1	5.5e-27	47.24	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot...	[more]
AT3G19010.1	2.2e-23	43.44	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot...	[more]
AT3G21420.1	9.4e-11	36.54	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot...	[more]
AT2G38240.1	2.1e-10	36.46	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot...	[more]
AT3G13610.1	3.6e-10	27.74	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot...	[more]

BLAST of Lsi11G010370 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659109825\|ref\|XP_008454901.1\|	1.7e-54	76.39	PREDICTED: hyoscyamine 6-dioxygenase-like isoform X2 [Cucumis melo]	[more]
gi\|659109823\|ref\|XP_008454900.1\|	1.7e-54	76.39	PREDICTED: hyoscyamine 6-dioxygenase-like isoform X1 [Cucumis melo]	[more]
gi\|659109829\|ref\|XP_008454903.1\|	2.2e-54	75.00	PREDICTED: LOW QUALITY PROTEIN: gibberellin 20 oxidase 1-like [Cucumis melo]	[more]
gi\|449438440\|ref\|XP_004136996.1\|	5.5e-53	73.97	PREDICTED: hyoscyamine 6-dioxygenase-like isoform X2 [Cucumis sativus]	[more]
gi\|413915332\|emb\|CBX88044.1\|	5.5e-53	73.97	gibberellin 20-oxidase, partial [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR027443	IPNS-like
IPR026992	DIOX_N

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016102	diterpenoid biosynthetic process
biological_process	GO:0055114	oxidation-reduction process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0016707	gibberellin 3-beta-dioxygenase activity
molecular_function	GO:0005506	iron ion binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi11G010370.1	Lsi11G010370.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 29..132 score: 7.0
IPR027443	Isopenicillin N synthase-like	GENE3D	G3DSA:2.60.120.330		coord: 4..133 score: 8.8
None	No IPR available	PANTHER	PTHR10209	OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN	coord: 4..133 score: 2.6
None	No IPR available	PANTHER	PTHR10209:SF98	SUBFAMILY NOT NAMED	coord: 4..133 score: 2.6
None	No IPR available	unknown	SSF51197	Clavaminate synthase-like	coord: 6..132 score: 1.58

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi11G010370	ClCG02G005610	Watermelon (Charleston Gray)	lsiwcgB097

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi11G010370	Bottle gourd (USVL1VR-Ls)	lsilsiB021
Lsi11G010370	Bottle gourd (USVL1VR-Ls)	lsilsiB047
Lsi11G010370	Cucumber (Gy14) v1	cgylsiB146
Lsi11G010370	Cucumber (Gy14) v1	cgylsiB361
Lsi11G010370	Cucurbita maxima (Rimu)	cmalsiB092
Lsi11G010370	Cucurbita maxima (Rimu)	cmalsiB472
Lsi11G010370	Cucurbita maxima (Rimu)	cmalsiB562
Lsi11G010370	Cucurbita maxima (Rimu)	cmalsiB784
Lsi11G010370	Cucurbita moschata (Rifu)	cmolsiB089
Lsi11G010370	Cucurbita moschata (Rifu)	cmolsiB457
Lsi11G010370	Cucurbita moschata (Rifu)	cmolsiB545
Lsi11G010370	Cucurbita pepo (Zucchini)	cpelsiB199
Lsi11G010370	Cucurbita pepo (Zucchini)	cpelsiB533
Lsi11G010370	Cucurbita pepo (Zucchini)	cpelsiB605
Lsi11G010370	Wild cucumber (PI 183967)	cpilsiB097
Lsi11G010370	Wild cucumber (PI 183967)	cpilsiB435
Lsi11G010370	Wild cucumber (PI 183967)	cpilsiB507
Lsi11G010370	Cucumber (Chinese Long) v2	culsiB102
Lsi11G010370	Cucumber (Chinese Long) v2	culsiB495
Lsi11G010370	Melon (DHL92) v3.5.1	lsimeB097
Lsi11G010370	Melon (DHL92) v3.5.1	lsimeB120
Lsi11G010370	Watermelon (Charleston Gray)	lsiwcgB075
Lsi11G010370	Watermelon (Charleston Gray)	lsiwcgB090
Lsi11G010370	Watermelon (97103) v1	lsiwmB088
Lsi11G010370	Watermelon (97103) v1	lsiwmB106
Lsi11G010370	Cucumber (Gy14) v2	cgyblsiB090
Lsi11G010370	Cucumber (Gy14) v2	cgyblsiB463
Lsi11G010370	Melon (DHL92) v3.6.1	lsimedB127
Lsi11G010370	Melon (DHL92) v3.6.1	lsimedB103
Lsi11G010370	Silver-seed gourd	carlsiB186
Lsi11G010370	Silver-seed gourd	carlsiB809
Lsi11G010370	Cucumber (Chinese Long) v3	cuclsiB109
Lsi11G010370	Cucumber (Chinese Long) v3	cuclsiB458
Lsi11G010370	Cucumber (Chinese Long) v3	cuclsiB530
Lsi11G010370	Watermelon (97103) v2	lsiwmbB086
Lsi11G010370	Watermelon (97103) v2	lsiwmbB108
Lsi11G010370	Wax gourd	lsiwgoB115