Lsi11G009330 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi11G009330
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Eukaryotic translation initiation factor 4H
Location	chr11 : 13387510 .. 13389578 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCACGGTTTGCATTTGATGGAGATCTGCAAGCGCCTTTTGCCTAGACTGCAGTTATGAAGCATGGTTATGCTACCGAATCCAAATGTGCATAGTGAGTACATAATGAGTTCAATTATGCATAACTTAAAAAAAAAAAAAAAAAACAGGAATTTGAAACTACAAAAAGAATTACTTAGGTAATCTATTCATCAATGAACAAGTCAATGTATCAACAAGCAAGGAAAGCATTAAATAAATTAATGGAAAACTTCGATTTTAAAATGTCCACTACCATAACAGAAAAAAGTTGAAATTGGGAAATGATGCGTTTAAAGAGAGAATTTTGTTTATCGATATATCAAGAAGGGAAAAAAATGTTCAAGGATACATGTTCCCAATGGGAATGGAAAAATACAAAAGAAAGAAGCTAAAAAATACAAGATAAAACAATGAAGAAAAACTAACAAAGCAAACAAGAACACAACCAATAAGCGGCAAAAAGAGCTCCACTCTAACTAAAACTTCAGAAGCTTCTTATGGTGAAAAATAGGCAGGAATCTCCAAATTGCAGCTTTTCCTTGAAAAATAAACCCATGGATAGAAATTTTCAATAATGCATTTCCTGCTGAAGAGATGGAAATCAAAGACTAATTTTACGATCCAAATTGTTGCACTAGTTTAATGCAGTTCTTGACAAAATTCTTCATTCCAACAGACACAAACAACCAGCTCGGGCTACTACTGATCAGGAAAAAAACAAGCTAGTCTTGAAAAAAAGGAATCATAAAAAGGATAGACCCATTTCTTAGAAACAAATAACCTGTCAAATAATGAGTGAGAAGCTGCATCTCCTACCCTTGACCATGTGAATAGAGGCAGTTTCAGCATACCAAGATCTTCAATATGATTGTTAAACTTTCTCATGCCCTTCATTTGCCTTCTAACTAGGTTCAAGTAGATTTAACCATTTTTTTGTCAAACATGACAATATTTATTGTCTTGAGTTAAAATCAAATATGAGCACTGATTTTGAAGAGATTTCTTTAAAACCGATTGCTTCGAAGCAACCAAGTTCTCTATTACTCCATGAAATTATCTTCATTGAGGAAATAACCAAAAAGAACCACTAAAATTTGATCTGAGGTGACACAGATGAAATTGCACGCATCCGAAGACTGAAATTTCAATAAAATATGATTTTTTTATTTTTTATTTTCTTTATTTTTATTTTTATTTTTATTTTTTTGAGGAATTGAGCGAGTGGACTCTTCTGGAGGCTCTTCAATGATCTGTTCATCAACAAACTTTAAAGAAGGGTCTACAGCTTCATCCAAAAATGTAGAGCAAAGGATTGTTCAAAATGATCATCCTCACAATTGGGCTTTAACTAGGAAACCTATGGCTGACGAGGTTGATAATTGGGCTTTAACTAAGAAACCTATGGCTGCAAATGGATTTGAGAGGAGGGAGAGAGGAGTAGGGTTTTCGGATTTGCAATCTTCAAAAGCCGACGAATCAGAGAGTTGGGTTTCTAATAAGAGCAATATTCCGTCAAATGGGAGGAGATTTGACAGAGAGCGACGATGAGGGTTCACGGCGAGCAGCAGTGGGCAGATTCAGACAATTGGGAGAGGAAGAAAAAGGAAGGTAACACTGGTGGCATCATTGGCGGTCAACCAAAGCTTAATTTGCAGCCATGGACACTGCCGGTTAGCGACAGGAAATCGTTGGAGATATCAGGGACGGTGGCAAGGTCCAAGAGTTCAAACCCATTTGGAGCGGTGAGGCCGAGGGAAGAGGTTCTAGCCAAGAAGGGACATGACTGGAAAAAGCTTGACAAGCAGCTAGAGTCGAAGATTAAGGAGGTGTCAGTGGAGGAGAAGACAGAAAAATCCAACGCCATTTCCTTTGGGAAAAAGAGCTTTAACAATAGAATAGAACAGAGAGGAGCTGGAGGAAGCCGAAGGCACTGCCGCAGGAGTCACCGGAATCTCTTCTTGACGGTGGGGAGGAATATGAAAATGGAGATTGCAAAGGCGAACGCAGAGGGGACTATAATGGAGCTTGATGTAACACTGAGAGATTGA

mRNA sequence

ATGCACGGAATTGAGCGAGTGGACTCTTCTGGAGGCTCTTCAATGATCTGTTCATCAACAAACTTTAAAGAAGGGTCTACAGCTTCATCCAAAAATGTAGAGCAAAGGATTGTTCAAAATGATCATCCTCACAATTGGGCTTTAACTAGGAAACCTATGGCTGACGAGGTTGATAATTGGGCTTTAACTAAGAAACCTATGGCTGCAAATGGATTTGAGAGGAGGGAGAGAGGAGTAGGGTTTTCGGATTTGCAATCTTCAAAAGCCGACGAATCAGAGAGTTGGAGAGCGACGATGAGGGTTCACGGCGAGCAGCAGTGGGCAGATTCAGACAATTGGGAGAGGAAGAAAAAGGAAGGTAACACTGGTGGCATCATTGGCGGTCAACCAAAGCTTAATTTGCAGCCATGGACACTGCCGGTTAGCGACAGGAAATCGTTGGAGATATCAGGGACGGTGGCAAGGTCCAAGAGTTCAAACCCATTTGGAGCGGTGAGGCCGAGGGAAGAGGTTCTAGCCAAGAAGGGACATGACTGGAAAAAGCTTGACAAGCAGCTAGAGTCGAAGATTAAGGAGGTGTCAGTGGAGGAGAAGACAGAAAAATCCAACGCCATTTCCTTTGGGAAAAAGAGCTTTAACAATAGAATAGAACAGAGAGGAGCTGGAGGAAGCCGAAGGCACTGCCGCAGGAGTCACCGGAATCTCTTCTTGACGGTGGGGAGGAATATGAAAATGGAGATTGCAAAGGCGAACGCAGAGGGGACTATAATGGAGCTTGATGTAACACTGAGAGATTGA

Coding sequence (CDS)

ATGCACGGAATTGAGCGAGTGGACTCTTCTGGAGGCTCTTCAATGATCTGTTCATCAACAAACTTTAAAGAAGGGTCTACAGCTTCATCCAAAAATGTAGAGCAAAGGATTGTTCAAAATGATCATCCTCACAATTGGGCTTTAACTAGGAAACCTATGGCTGACGAGGTTGATAATTGGGCTTTAACTAAGAAACCTATGGCTGCAAATGGATTTGAGAGGAGGGAGAGAGGAGTAGGGTTTTCGGATTTGCAATCTTCAAAAGCCGACGAATCAGAGAGTTGGAGAGCGACGATGAGGGTTCACGGCGAGCAGCAGTGGGCAGATTCAGACAATTGGGAGAGGAAGAAAAAGGAAGGTAACACTGGTGGCATCATTGGCGGTCAACCAAAGCTTAATTTGCAGCCATGGACACTGCCGGTTAGCGACAGGAAATCGTTGGAGATATCAGGGACGGTGGCAAGGTCCAAGAGTTCAAACCCATTTGGAGCGGTGAGGCCGAGGGAAGAGGTTCTAGCCAAGAAGGGACATGACTGGAAAAAGCTTGACAAGCAGCTAGAGTCGAAGATTAAGGAGGTGTCAGTGGAGGAGAAGACAGAAAAATCCAACGCCATTTCCTTTGGGAAAAAGAGCTTTAACAATAGAATAGAACAGAGAGGAGCTGGAGGAAGCCGAAGGCACTGCCGCAGGAGTCACCGGAATCTCTTCTTGACGGTGGGGAGGAATATGAAAATGGAGATTGCAAAGGCGAACGCAGAGGGGACTATAATGGAGCTTGATGTAACACTGAGAGATTGA

Protein sequence

MHGIERVDSSGGSSMICSSTNFKEGSTASSKNVEQRIVQNDHPHNWALTRKPMADEVDNWALTKKPMAANGFERRERGVGFSDLQSSKADESESWRATMRVHGEQQWADSDNWERKKKEGNTGGIIGGQPKLNLQPWTLPVSDRKSLEISGTVARSKSSNPFGAVRPREEVLAKKGHDWKKLDKQLESKIKEVSVEEKTEKSNAISFGKKSFNNRIEQRGAGGSRRHCRRSHRNLFLTVGRNMKMEIAKANAEGTIMELDVTLRD

Homology

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BLAST of Lsi11G009330 vs. Swiss-Prot
Match: IF4B3_ARATH (Eukaryotic translation initiation factor 4B3 OS=Arabidopsis thaliana GN=EIF4B3 PE=1 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 1.8e-14
Identity = 73/235 (31.06%), Postives = 112/235 (47.66%), Query Frame = 1


Query: 41  DHPHNWALTRKPM------------------------ADEVDNWALTKKP-----MAANG 100
           D   NWA  +KP+                        ADEVD+W  TK       +++NG
Sbjct: 164 DEDDNWAAAKKPISGNGFERRERGSGGGFFESQSQSKADEVDSWVSTKPSEPRRFVSSNG 223

Query: 101 F--ERRERGVGFSDLQSSKADESESWRATMRVHGEQQWADSDNWERKKKE-----GNTGG 160
              +R E+   F  L  ++  +          +G    ++SD W R+++E     G+   
Sbjct: 224 GGGDRFEKRGSFESLSRNRDSQ----------YGGGGGSESDTWGRRREESGAANGSPPP 283

Query: 161 IIGGQPKLNLQPWTLPVSDRKSLE----ISGTVARSKSSNPFGAVRPREEVLAKKGHDWK 220
             G +P+L LQP TLPV+  + ++    +   V + K +NPFG  RPREEVLA+KG DWK
Sbjct: 284 SGGSRPRLVLQPRTLPVAVVEVVKPESPVLVIVEKPKGANPFGNARPREEVLAEKGQDWK 343

Query: 221 KLDKQLES-KIKEVSVEEKTEKSNAISFGKKSFNNRIEQRGAGGSRRHCRRSHRN 235
           ++D++LE+ K+K+++     EK N  S GK  F       G G  R+   R  R+
Sbjct: 344 EIDEKLEAEKLKDIAA--AMEKPNEKSTGKMGF-------GLGNGRKDEERIERS 379

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BLAST of Lsi11G009330 vs. Swiss-Prot
Match: IF4B1_WHEAT (Eukaryotic translation initiation factor 4B1 OS=Triticum aestivum GN=EIF4B PE=1 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 1.7e-09
Identity = 59/181 (32.60%), Postives = 89/181 (49.17%), Query Frame = 1


Query: 54  ADEVDNWALTKKPMAAN-------GFERRERGVGFSDLQSSKADESESWRATMRVHGEQQ 113
           +D++DNW+  KKP+ +        G  R   G GF +  SS     ES     R      
Sbjct: 182 SDDIDNWSRDKKPLPSRYPSLGTGGGFRESSGGGFRE--SSGGGFRESSGGGFR--DSPG 241

Query: 114 WADSDNWERKKKEGNTGGIIGGQPKLNLQPWTLPVSDRKSLEI-SGTVARSKSSNPFGAV 173
            +DSD W R           G +P+LNL P   P  D  +  + +  VARS+ S PFGA 
Sbjct: 242 PSDSDRWVRGAVPAPMTNN-GDRPRLNLNP---PKRDPSATPVPAAEVARSRPS-PFGAA 301

Query: 174 RPREEVLAKKGHDWKKLDKQLESKIKEVSVEEKTEKSNAISFGKKSFNNRIEQRGAGGSR 227
           +PREEVLA+KG DW+K++ ++E K    +    +  ++A S    S  +++   G+ G+ 
Sbjct: 302 KPREEVLAEKGLDWRKMEGEIEKKTSRPTSSHSSRPNSAHSSRPGSPGSQVSAVGSEGAP 353

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BLAST of Lsi11G009330 vs. Swiss-Prot
Match: IF4B1_ARATH (Eukaryotic translation initiation factor 4B1 OS=Arabidopsis thaliana GN=EIF4B1 PE=1 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 1.2e-07
Identity = 58/189 (30.69%), Postives = 87/189 (46.03%), Query Frame = 1


Query: 54  ADEVDNWALTKKPMAANGFERRER----GVGFSDLQS-------------SKADESESWR 113
           ADEVD+W   KKP+ +    R+ R    G GF    S             S+AD+ ++W 
Sbjct: 168 ADEVDDWGKEKKPLPSFDQGRQGRYSGDGGGFGGGGSGFGGGGGGGGGGLSRADDVDNWG 227

Query: 114 ATMRVHGEQQWADSDNWERKKKEGNTGGIIGGQPKLNLQPWTLPVSDRKSLEISGT---- 173
           A     G++Q        R    G++ G  G + +  L      V + + +E  G+    
Sbjct: 228 A-----GKRQAP-----VRSSTFGSSFGDSGQEERRRL------VLEPRKVESGGSETPP 287

Query: 174 -VARSKSSNPFGAVRPREEVLAKKGHDWKKLDKQLESKIKEVSVEEKTEKSNAISFGKKS 221
            V ++   NPFGA RPRE+VLA+KG DWKK+D ++E+K         T   ++     +S
Sbjct: 288 VVEKTSKPNPFGAARPREDVLAEKGLDWKKIDSEIEAKKGSSQTSRPTSAHSSRPSSAQS 338

[Close]

BLAST of Lsi11G009330 vs. Swiss-Prot
Match: IF4B2_ARATH (Eukaryotic translation initiation factor 4B2 OS=Arabidopsis thaliana GN=EIF4B2 PE=1 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 4.7e-07
Identity = 49/155 (31.61%), Postives = 65/155 (41.94%), Query Frame = 1


Query: 54  ADEVDNWALTKKPMAANGFERRERGVGFSDLQSSKADESESWRATMRVHGEQQWADSDNW 113
           ADEVDNWA  K   +  G   RE G                              + D W
Sbjct: 221 ADEVDNWAAGKAKSSTFGSGFRESG-----------------------------PEPDRW 280

Query: 114 ERKKKEGNTGGIIGGQPKLNLQPWTLPVSDRKSLEISGTVARSKSS--NPFGAVRPREEV 173
            R       GG+   + +L  +P       +   E+S T    K+S  +PFGA RPRE+V
Sbjct: 281 ARGVLPSG-GGVQEERRRLVFEP------RKADTEVSETPTAVKTSKPSPFGAARPREQV 339

Query: 174 LAKKGHDWKKLDKQLESKIKEVSVEEKTEKSNAIS 207
           LA+KG DWKKLD  +E+K  + S     + S   S
Sbjct: 341 LAEKGLDWKKLDSDIEAKKGQTSRPSSAQSSRPSS 339

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BLAST of Lsi11G009330 vs. TrEMBL
Match: A0A0A0K4M1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G009760 PE=4 SV=1)

HSP 1 Score: 229.6 bits (584), Expect = 4.6e-57
Identity = 136/228 (59.65%), Postives = 156/228 (68.42%), Query Frame = 1


Query: 19  STNFKEGSTASSKNVEQRIVQNDHPHNWALTRKPMADEVDNWALTKKPMAANGFERRERG 78
           S+N K GS+  S    +   + D    +  +R   ADEVDNWALTKKPMA NGFERRERG
Sbjct: 123 SSNSKWGSSRVSDENRRSSNRADSSKEFTSSR---ADEVDNWALTKKPMAGNGFERRERG 182

Query: 79  VGFSDLQSSKADESESWRAT--------MRVHGEQQ--------WADSDNWERKKKEGNT 138
           +GFSD QSSKADESESW +          R   E++         ADSDNW RKK+EGN 
Sbjct: 183 LGFSDSQSSKADESESWVSNKSNIPSDGRRFDRERRGGFTASGGGADSDNWGRKKEEGNA 242

Query: 139 GGIIGGQPKLNLQPWTLPVSDRKSLEISGTVARSKSSNPFGAVRPREEVLAKKGHDWKKL 198
           GG+I  +PKL+LQP TLPV D KS EIS T ARSKSSNPFGA RPREEVLA+KGHDWKKL
Sbjct: 243 GGVISSRPKLSLQPRTLPVVDGKSSEISATAARSKSSNPFGAARPREEVLAEKGHDWKKL 302

Query: 199 DKQLESKIKEVSVEEKTEKSNAISFGKKSFNNRIEQRGAGGSRRHCRR 231
           D+QLESKIKEVSVEEKTEKSN I F K+SF N   +     + R  R+
Sbjct: 303 DEQLESKIKEVSVEEKTEKSNPIFFRKRSFGNGNSRAAEDRTERSWRK 347

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BLAST of Lsi11G009330 vs. TrEMBL
Match: A0A067JUK2_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_19582 PE=4 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 4.9e-27
Identity = 90/209 (43.06%), Postives = 117/209 (55.98%), Query Frame = 1


Query: 54  ADEVDNWALTKKPMAANGFERRERGVGFSDLQSSKADESESWRATMRV------------ 113
           ADE+D+W+ TKK    NG+ERRERG  F  L  SKADES+SW A                
Sbjct: 155 ADEIDDWSKTKKSPVGNGYERRERGSFFDSL--SKADESDSWVANKPTESRRFGATNGGF 214

Query: 114 -------------HGEQQ---WADSDNWERKKKEGNTGGIIGGQPKLNLQPWTLPVSDRK 173
                        HG       ADSD+W RK+++ N  G    +PKL LQP +LPV+D  
Sbjct: 215 ERRGSFDSLSRDRHGSNSGAGGADSDSWGRKREDTNITGT--ARPKLVLQPRSLPVND-- 274

Query: 174 SLEISGTVARSKSSNPFGAVRPREEVLAKKGHDWKKLDKQLESKIKEVSVEEKTEKSNAI 233
               +GT A+ K SNPFG  RPREEVLA+KG DWK++D++LES   +++  ++ E+ N+ 
Sbjct: 275 ----NGTAAKPKGSNPFGDARPREEVLAEKGKDWKEIDEKLES--VKINGNKEMERGNST 334

[Close]

BLAST of Lsi11G009330 vs. TrEMBL
Match: A0A0A0LJC8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G263980 PE=4 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 4.9e-27
Identity = 86/200 (43.00%), Postives = 108/200 (54.00%), Query Frame = 1


Query: 54  ADEVDNWALTKKPMAANGFERRER--GVGFSDLQSSKADESESWRATMRVHGEQ------ 113
           ADE+D+W   KKPM  NGFERRER  G GF D  SSKADES+SW ++      +      
Sbjct: 168 ADEIDDWGAGKKPMVGNGFERRERGGGGGFFDSHSSKADESDSWVSSKSFTPSEGRRSGG 227

Query: 114 -------------QWADSDNWERKKKEGNTGGI----------------------IGGQP 173
                          ADSDNW R K +G  GGI                      IG +P
Sbjct: 228 FDRERRGGFPTSGGGADSDNWGR-KPDGARGGIGENGGSADSENWGKRSEGVRSGIGERP 287

Query: 174 KLNLQPWTLPVSDRKSLEISGTVARSKSSNPFGAVRPREEVLAKKGHDWKKLDKQLESKI 211
           +LNLQP ++P+++    E SG   + K SNPFG  RPREEVLA+KG DWKK+D+QLES +
Sbjct: 288 RLNLQPRSIPLNNGNQ-EASGVAVKPKGSNPFGNARPREEVLAEKGQDWKKIDEQLES-V 347

[Close]

BLAST of Lsi11G009330 vs. TrEMBL
Match: E5GC13_CUCME (Translation initiation factor OS=Cucumis melo subsp. melo PE=4 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 3.5e-25
Identity = 86/201 (42.79%), Postives = 108/201 (53.73%), Query Frame = 1


Query: 54  ADEVDNWALTKKPMAANGFERRER--GVGFSDLQSSKADESESWRATMRVHGEQ------ 113
           ADE+D+W   KKPM  NGFERRER  G GF D  SSKADES+SW ++      +      
Sbjct: 168 ADEIDDWGAGKKPMMGNGFERRERGGGGGFFDSHSSKADESDSWVSSKSFTPSEGRRSGG 227

Query: 114 -------------QWADSDNWER----------------------KKKEGNTGGIIGGQP 173
                          ADSDNW R                      KK EG   G IG +P
Sbjct: 228 FDRERRGGFPTSGGGADSDNWGRKSDGARAGMGENGGGADSDNWGKKSEGVRSG-IGERP 287

Query: 174 KLNLQPWTLPVSDRKSLEISGTVARSKSSNPFGAVRPREEVLAKKGHDWKKLDKQLES-K 211
           +LNLQP ++P+++    E SG   + K SNPFG  RPREEVLA+KG DWKK+D+QL S K
Sbjct: 288 RLNLQPRSIPLNNGNQ-EASGVAVKPKGSNPFGNARPREEVLAEKGQDWKKIDEQLGSMK 347

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BLAST of Lsi11G009330 vs. TrEMBL
Match: F6I126_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_03s0038g01950 PE=4 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 1.0e-24
Identity = 82/191 (42.93%), Postives = 110/191 (57.59%), Query Frame = 1


Query: 54  ADEVDNWALTKKPMAANGFERRERGVGFSDLQSSKADESESWRATMRVHGEQ-------- 113
           ADE+D+W   KK    NGFERR+RG GF D Q S+ADES SW +       +        
Sbjct: 176 ADEIDDWGAAKKSTVGNGFERRDRG-GFFDSQ-SRADESASWVSNKSFTPSEGRRFGGGG 235

Query: 114 -------------------QWADSDNWERKKKE--GNTGGIIGGQPKLNLQPWTLPVSDR 173
                                ADS++W RKK+E  GN  G  G +PKL LQP T+PV+D 
Sbjct: 236 GFESLRERRGGFDSASDGGGGADSESWGRKKEEGSGNANGSAGSRPKLILQPRTVPVNDG 295

Query: 174 KSLEISGTVARSKSSNPFGAVRPREEVLAKKGHDWKKLDKQLES-KIKEVSVEEKTEKSN 215
           +    SG+VA+ K  NPFG  RPREEVLA+KG DWK+++++LES K+K+V       +++
Sbjct: 296 QQ-PGSGSVAKPKGPNPFGEARPREEVLAEKGQDWKEIEEKLESVKLKDVG-SPGVGQTD 355

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BLAST of Lsi11G009330 vs. TAIR10
Match: AT4G38710.2 (AT4G38710.2 glycine-rich protein)

HSP 1 Score: 84.7 bits (208), Expect = 9.2e-17
Identity = 77/254 (30.31%), Postives = 119/254 (46.85%), Query Frame = 1


Query: 41  DHPHNWALTRKPM------------------------ADEVDNWALTKKP-----MAANG 100
           D   NWA  +KP+                        ADEVD+W  TK       +++NG
Sbjct: 164 DEDDNWAAAKKPISGNGFERRERGSGGGFFESQSQSKADEVDSWVSTKPSEPRRFVSSNG 223

Query: 101 F--ERRERGVGFSDLQSSKADESESWRATMRVHGEQQWADSDNWERKKKE-----GNTGG 160
              +R E+   F  L  ++  +          +G    ++SD W R+++E     G+   
Sbjct: 224 GGGDRFEKRGSFESLSRNRDSQ----------YGGGGGSESDTWGRRREESGAANGSPPP 283

Query: 161 IIGGQPKLNLQPWTLPVSDRKSLE----ISGTVARSKSSNPFGAVRPREEVLAKKGHDWK 220
             G +P+L LQP TLPV+  + ++    +   V + K +NPFG  RPREEVLA+KG DWK
Sbjct: 284 SGGSRPRLVLQPRTLPVAVVEVVKPESPVLVIVEKPKGANPFGNARPREEVLAEKGQDWK 343

Query: 221 KLDKQLES-KIKEVSVEEKTEKSNAISFGKKSFNNRIEQRGAGGSRRHCRRSHRNLFLTV 254
           ++D++LE+ K+K+++     EK N  S GK  F       G G  R+   R  R+   + 
Sbjct: 344 EIDEKLEAEKLKDIAA--AMEKPNEKSTGKMGF-------GLGNGRKDEERIERSWRKSF 398

[Close]

BLAST of Lsi11G009330 vs. TAIR10
Match: AT3G26400.1 (AT3G26400.1 eukaryotic translation initiation factor 4B1)

HSP 1 Score: 58.5 bits (140), Expect = 7.0e-09
Identity = 58/189 (30.69%), Postives = 87/189 (46.03%), Query Frame = 1


Query: 54  ADEVDNWALTKKPMAANGFERRER----GVGFSDLQS-------------SKADESESWR 113
           ADEVD+W   KKP+ +    R+ R    G GF    S             S+AD+ ++W 
Sbjct: 168 ADEVDDWGKEKKPLPSFDQGRQGRYSGDGGGFGGGGSGFGGGGGGGGGGLSRADDVDNWG 227

Query: 114 ATMRVHGEQQWADSDNWERKKKEGNTGGIIGGQPKLNLQPWTLPVSDRKSLEISGT---- 173
           A     G++Q        R    G++ G  G + +  L      V + + +E  G+    
Sbjct: 228 A-----GKRQAP-----VRSSTFGSSFGDSGQEERRRL------VLEPRKVESGGSETPP 287

Query: 174 -VARSKSSNPFGAVRPREEVLAKKGHDWKKLDKQLESKIKEVSVEEKTEKSNAISFGKKS 221
            V ++   NPFGA RPRE+VLA+KG DWKK+D ++E+K         T   ++     +S
Sbjct: 288 VVEKTSKPNPFGAARPREDVLAEKGLDWKKIDSEIEAKKGSSQTSRPTSAHSSRPSSAQS 338

[Close]

BLAST of Lsi11G009330 vs. TAIR10
Match: AT1G13020.1 (AT1G13020.1 eukaryotic initiation factor 4B2)

HSP 1 Score: 56.6 bits (135), Expect = 2.7e-08
Identity = 49/155 (31.61%), Postives = 65/155 (41.94%), Query Frame = 1


Query: 54  ADEVDNWALTKKPMAANGFERRERGVGFSDLQSSKADESESWRATMRVHGEQQWADSDNW 113
           ADEVDNWA  K   +  G   RE G                              + D W
Sbjct: 221 ADEVDNWAAGKAKSSTFGSGFRESG-----------------------------PEPDRW 280

Query: 114 ERKKKEGNTGGIIGGQPKLNLQPWTLPVSDRKSLEISGTVARSKSS--NPFGAVRPREEV 173
            R       GG+   + +L  +P       +   E+S T    K+S  +PFGA RPRE+V
Sbjct: 281 ARGVLPSG-GGVQEERRRLVFEP------RKADTEVSETPTAVKTSKPSPFGAARPREQV 339

Query: 174 LAKKGHDWKKLDKQLESKIKEVSVEEKTEKSNAIS 207
           LA+KG DWKKLD  +E+K  + S     + S   S
Sbjct: 341 LAEKGLDWKKLDSDIEAKKGQTSRPSSAQSSRPSS 339

[Close]

BLAST of Lsi11G009330 vs. NCBI nr
Match: gi|659094279|ref|XP_008447975.1| (PREDICTED: dentin sialophosphoprotein-like [Cucumis melo])

HSP 1 Score: 236.9 bits (603), Expect = 4.1e-59
Identity = 135/212 (63.68%), Postives = 153/212 (72.17%), Query Frame = 1


Query: 19  STNFKEGSTASSKNVEQRIVQNDHPHNWALTRKPMADEVDNWALTKKPMAANGFERRERG 78
           S+N K GS+  S    +    ++   +        ADEVDNWALTKKPMA NGFERRERG
Sbjct: 123 SSNSKWGSSRVSDENRRSSNGSNRADSSKEFTSSRADEVDNWALTKKPMAGNGFERRERG 182

Query: 79  VGFSDLQSSKADESESWRAT--------MRVHGEQQ--------WADSDNWERKKKEGNT 138
           +GFSD QSSKADESESW +          R   E++         ADSDNW RKK+EGN 
Sbjct: 183 LGFSDSQSSKADESESWVSNKSNIPSGGRRFDRERRGGFTASGGGADSDNWGRKKEEGNA 242

Query: 139 GGIIGGQPKLNLQPWTLPVSDRKSLEISGTVARSKSSNPFGAVRPREEVLAKKGHDWKKL 198
           GG+IG +PKL+LQP TLPV D KS EIS T ARSKSSNPFGA RPREEVLA+KGHDWKKL
Sbjct: 243 GGVIGSRPKLSLQPRTLPVGDGKSSEISATAARSKSSNPFGAARPREEVLAEKGHDWKKL 302

Query: 199 DKQLESKIKEVSVEEKTEKSNAISFGKKSFNN 215
           D+QLESKIKEVSVEEKTEKSNAISF K+SF+N
Sbjct: 303 DEQLESKIKEVSVEEKTEKSNAISFRKRSFDN 334

[Close]

BLAST of Lsi11G009330 vs. NCBI nr
Match: gi|449454189|ref|XP_004144838.1| (PREDICTED: uncharacterized protein LOC101206224 [Cucumis sativus])

HSP 1 Score: 229.6 bits (584), Expect = 6.5e-57
Identity = 136/228 (59.65%), Postives = 156/228 (68.42%), Query Frame = 1


Query: 19  STNFKEGSTASSKNVEQRIVQNDHPHNWALTRKPMADEVDNWALTKKPMAANGFERRERG 78
           S+N K GS+  S    +   + D    +  +R   ADEVDNWALTKKPMA NGFERRERG
Sbjct: 123 SSNSKWGSSRVSDENRRSSNRADSSKEFTSSR---ADEVDNWALTKKPMAGNGFERRERG 182

Query: 79  VGFSDLQSSKADESESWRAT--------MRVHGEQQ--------WADSDNWERKKKEGNT 138
           +GFSD QSSKADESESW +          R   E++         ADSDNW RKK+EGN 
Sbjct: 183 LGFSDSQSSKADESESWVSNKSNIPSDGRRFDRERRGGFTASGGGADSDNWGRKKEEGNA 242

Query: 139 GGIIGGQPKLNLQPWTLPVSDRKSLEISGTVARSKSSNPFGAVRPREEVLAKKGHDWKKL 198
           GG+I  +PKL+LQP TLPV D KS EIS T ARSKSSNPFGA RPREEVLA+KGHDWKKL
Sbjct: 243 GGVISSRPKLSLQPRTLPVVDGKSSEISATAARSKSSNPFGAARPREEVLAEKGHDWKKL 302

Query: 199 DKQLESKIKEVSVEEKTEKSNAISFGKKSFNNRIEQRGAGGSRRHCRR 231
           D+QLESKIKEVSVEEKTEKSN I F K+SF N   +     + R  R+
Sbjct: 303 DEQLESKIKEVSVEEKTEKSNPIFFRKRSFGNGNSRAAEDRTERSWRK 347

[Close]

BLAST of Lsi11G009330 vs. NCBI nr
Match: gi|1009151201|ref|XP_015893428.1| (PREDICTED: eukaryotic translation initiation factor 4B3 [Ziziphus jujuba])

HSP 1 Score: 132.1 bits (331), Expect = 1.4e-27
Identity = 94/210 (44.76%), Postives = 118/210 (56.19%), Query Frame = 1


Query: 54  ADEVDNWALTKKPMAANGFERRERGVGFSDLQSSKADESESW------------------ 113
           ADE+DNWA  KK    NGFER++RG GF D Q SKADES+SW                  
Sbjct: 165 ADEIDNWAAAKKSPTRNGFERKDRG-GFFDSQ-SKADESDSWVSNKSFVPSEGRRFGSSG 224

Query: 114 -------RATMRVHGEQQWADSDNWERKKKEG----NTGGIIGGQPKLNLQPWTLPVSDR 173
                  R +   +G    ADSDNW +K++E        G   G+P+LNLQP TLPVSD 
Sbjct: 225 GGFERERRVSFTSNG--GGADSDNWGKKREESYGVTTVNGTESGRPRLNLQPRTLPVSDG 284

Query: 174 KSL-EISGTVARSKSSNPFGAVRPREEVLAKKGHDWKKLDKQLESKIKEVSVEEKTEKSN 233
            S    +  VA++K SNPFG  RPREEVLA+KG DWKK+D+QLESK+K  +  +K     
Sbjct: 285 GSQGSATVVVAKTKGSNPFGDARPREEVLAEKGQDWKKIDEQLESKVKHTN--DKAADGG 344

[Close]

BLAST of Lsi11G009330 vs. NCBI nr
Match: gi|802707720|ref|XP_012084371.1| (PREDICTED: uncharacterized protein LOC105643776 [Jatropha curcas])

HSP 1 Score: 129.8 bits (325), Expect = 7.1e-27
Identity = 90/209 (43.06%), Postives = 117/209 (55.98%), Query Frame = 1


Query: 54  ADEVDNWALTKKPMAANGFERRERGVGFSDLQSSKADESESWRATMRV------------ 113
           ADE+D+W+ TKK    NG+ERRERG  F  L  SKADES+SW A                
Sbjct: 155 ADEIDDWSKTKKSPVGNGYERRERGSFFDSL--SKADESDSWVANKPTESRRFGATNGGF 214

Query: 114 -------------HGEQQ---WADSDNWERKKKEGNTGGIIGGQPKLNLQPWTLPVSDRK 173
                        HG       ADSD+W RK+++ N  G    +PKL LQP +LPV+D  
Sbjct: 215 ERRGSFDSLSRDRHGSNSGAGGADSDSWGRKREDTNITGT--ARPKLVLQPRSLPVND-- 274

Query: 174 SLEISGTVARSKSSNPFGAVRPREEVLAKKGHDWKKLDKQLESKIKEVSVEEKTEKSNAI 233
               +GT A+ K SNPFG  RPREEVLA+KG DWK++D++LES   +++  ++ E+ N+ 
Sbjct: 275 ----NGTAAKPKGSNPFGDARPREEVLAEKGKDWKEIDEKLES--VKINGNKEMERGNST 334

[Close]

BLAST of Lsi11G009330 vs. NCBI nr
Match: gi|449458574|ref|XP_004147022.1| (PREDICTED: eukaryotic translation initiation factor 4B-like [Cucumis sativus])

HSP 1 Score: 129.8 bits (325), Expect = 7.1e-27
Identity = 86/200 (43.00%), Postives = 108/200 (54.00%), Query Frame = 1


Query: 54  ADEVDNWALTKKPMAANGFERRER--GVGFSDLQSSKADESESWRATMRVHGEQ------ 113
           ADE+D+W   KKPM  NGFERRER  G GF D  SSKADES+SW ++      +      
Sbjct: 168 ADEIDDWGAGKKPMVGNGFERRERGGGGGFFDSHSSKADESDSWVSSKSFTPSEGRRSGG 227

Query: 114 -------------QWADSDNWERKKKEGNTGGI----------------------IGGQP 173
                          ADSDNW R K +G  GGI                      IG +P
Sbjct: 228 FDRERRGGFPTSGGGADSDNWGR-KPDGARGGIGENGGSADSENWGKRSEGVRSGIGERP 287

Query: 174 KLNLQPWTLPVSDRKSLEISGTVARSKSSNPFGAVRPREEVLAKKGHDWKKLDKQLESKI 211
           +LNLQP ++P+++    E SG   + K SNPFG  RPREEVLA+KG DWKK+D+QLES +
Sbjct: 288 RLNLQPRSIPLNNGNQ-EASGVAVKPKGSNPFGNARPREEVLAEKGQDWKKIDEQLES-V 347

The following BLAST results are available for this feature:

BLAST of Lsi11G009330 vs. Swiss-Prot


Match Name	E-value	Identity	Description
IF4B3_ARATH	1.8e-14	31.06	Eukaryotic translation initiation factor 4B3 OS=Arabidopsis thaliana GN=EIF4B3 P...	[more]
IF4B1_WHEAT	1.7e-09	32.60	Eukaryotic translation initiation factor 4B1 OS=Triticum aestivum GN=EIF4B PE=1 ...	[more]
IF4B1_ARATH	1.2e-07	30.69	Eukaryotic translation initiation factor 4B1 OS=Arabidopsis thaliana GN=EIF4B1 P...	[more]
IF4B2_ARATH	4.7e-07	31.61	Eukaryotic translation initiation factor 4B2 OS=Arabidopsis thaliana GN=EIF4B2 P...	[more]

BLAST of Lsi11G009330 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K4M1_CUCSA	4.6e-57	59.65	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G009760 PE=4 SV=1	[more]
A0A067JUK2_JATCU	4.9e-27	43.06	Uncharacterized protein OS=Jatropha curcas GN=JCGZ_19582 PE=4 SV=1	[more]
A0A0A0LJC8_CUCSA	4.9e-27	43.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G263980 PE=4 SV=1	[more]
E5GC13_CUCME	3.5e-25	42.79	Translation initiation factor OS=Cucumis melo subsp. melo PE=4 SV=1	[more]
F6I126_VITVI	1.0e-24	42.93	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_03s0038g01950 PE=4 SV=...	[more]

BLAST of Lsi11G009330 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G38710.2	9.2e-17	30.31	glycine-rich protein	[more]
AT3G26400.1	7.0e-09	30.69	eukaryotic translation initiation factor 4B1	[more]
AT1G13020.1	2.7e-08	31.61	eukaryotic initiation factor 4B2	[more]

BLAST of Lsi11G009330 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659094279\|ref\|XP_008447975.1\|	4.1e-59	63.68	PREDICTED: dentin sialophosphoprotein-like [Cucumis melo]	[more]
gi\|449454189\|ref\|XP_004144838.1\|	6.5e-57	59.65	PREDICTED: uncharacterized protein LOC101206224 [Cucumis sativus]	[more]
gi\|1009151201\|ref\|XP_015893428.1\|	1.4e-27	44.76	PREDICTED: eukaryotic translation initiation factor 4B3 [Ziziphus jujuba]	[more]
gi\|802707720\|ref\|XP_012084371.1\|	7.1e-27	43.06	PREDICTED: uncharacterized protein LOC105643776 [Jatropha curcas]	[more]
gi\|449458574\|ref\|XP_004147022.1\|	7.1e-27	43.00	PREDICTED: eukaryotic translation initiation factor 4B-like [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003743	translation initiation factor activity

Vocabulary: **INTERPRO**
Term	Definition
IPR010433	EIF-4B_pln

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function
molecular_function	GO:0003743	translation initiation factor activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi11G009330.1	Lsi11G009330.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR010433	Plant specific eukaryotic initiation factor 4B	PFAM	PF06273	eIF-4B	coord: 49..190 score: 2.6
None	No IPR available	PANTHER	PTHR32091	FAMILY NOT NAMED	coord: 54..226 score: 4.1
None	No IPR available	PANTHER	PTHR32091:SF5	GLYCINE-RICH PROTEIN	coord: 54..226 score: 4.1

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi11G009330	Cucumber (Gy14) v1	cgylsiB450
Lsi11G009330	Cucurbita maxima (Rimu)	cmalsiB154
Lsi11G009330	Cucurbita maxima (Rimu)	cmalsiB709
Lsi11G009330	Cucurbita moschata (Rifu)	cmolsiB143
Lsi11G009330	Cucurbita moschata (Rifu)	cmolsiB703
Lsi11G009330	Cucurbita pepo (Zucchini)	cpelsiB006
Lsi11G009330	Cucurbita pepo (Zucchini)	cpelsiB654
Lsi11G009330	Wild cucumber (PI 183967)	cpilsiB096
Lsi11G009330	Cucumber (Chinese Long) v2	culsiB098
Lsi11G009330	Melon (DHL92) v3.5.1	lsimeB115
Lsi11G009330	Melon (DHL92) v3.5.1	lsimeB117
Lsi11G009330	Watermelon (Charleston Gray)	lsiwcgB083
Lsi11G009330	Watermelon (97103) v1	lsiwmB113
Lsi11G009330	Cucumber (Gy14) v2	cgyblsiB089
Lsi11G009330	Melon (DHL92) v3.6.1	lsimedB123
Lsi11G009330	Melon (DHL92) v3.6.1	lsimedB125
Lsi11G009330	Silver-seed gourd	carlsiB436
Lsi11G009330	Cucumber (Chinese Long) v3	cuclsiB108
Lsi11G009330	Watermelon (97103) v2	lsiwmbB078
Lsi11G009330	Wax gourd	lsiwgoB110
Lsi11G009330	Wax gourd	lsiwgoB111