Lsi11G006350 (gene) Bottle gourd (USVL1VR-Ls)

Overview
NameLsi11G006350
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionNicotianamine synthase
Locationchr11 : 6701708 .. 6702187 (+)
Genome Viewer
Gene Expression
   



Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTAGTCAATTGGTTTGCTCTGACCCAGACTTATCACAAAGAATGATATTTCACACAACAGATGTAATGGAAGTAACAAAGGGATTAAAAGATTATGAAGTAGTTTTCTTAGCAGCATTGGTTGGATTGGAAGAGGAAGAAAAAGGCAGAGTTTTGAAGCATTTGGGGAAGCATATGGCAGCTGGTGCTTATTTGATGCTAAGAAGTGCGCATGGAGCTAGAGCATTTTTATACCCTGTTGTTGATATTTGTAATGTTGAAGCTTCTGGATTTGAGATTTTATCTGTGTTTCATCCCACTGATGAAGTTATAAACTCTGTTGTTATTGCAAGGAAGAAAGTAGTGTTTGATCGAAACACGGTCGACGAGAACGACGAGAAGCGAGGTATTTTAGTTAATACCTCCTCTATTGTGTTGGCTGAGAAATGCTCTGGGTTTAATGGCTTTAGTCCCATGATTGAAGAGCAACTTTCTTGA

mRNA sequence

ATGGCTAGTCAATTGGTTTGCTCTGACCCAGACTTATCACAAAGAATGATATTTCACACAACAGATGTAATGGAAGTAACAAAGGGATTAAAAGATTATGAAGTAGTTTTCTTAGCAGCATTGGTTGGATTGGAAGAGGAAGAAAAAGGCAGAGTTTTGAAGCATTTGGGGAAGCATATGGCAGCTGGTGCTTATTTGATGCTAAGAAGTGCGCATGGAGCTAGAGCATTTTTATACCCTGTTGTTGATATTTGTAATGTTGAAGCTTCTGGATTTGAGATTTTATCTGTGTTTCATCCCACTGATGAAGTTATAAACTCTGTTGTTATTGCAAGGAAGAAAGTAGTGTTTGATCGAAACACGGTCGACGAGAACGACGAGAAGCGAGGTATTTTAGTTAATACCTCCTCTATTGTGTTGGCTGAGAAATGCTCTGGGTTTAATGGCTTTAGTCCCATGATTGAAGAGCAACTTTCTTGA

Coding sequence (CDS)

ATGGCTAGTCAATTGGTTTGCTCTGACCCAGACTTATCACAAAGAATGATATTTCACACAACAGATGTAATGGAAGTAACAAAGGGATTAAAAGATTATGAAGTAGTTTTCTTAGCAGCATTGGTTGGATTGGAAGAGGAAGAAAAAGGCAGAGTTTTGAAGCATTTGGGGAAGCATATGGCAGCTGGTGCTTATTTGATGCTAAGAAGTGCGCATGGAGCTAGAGCATTTTTATACCCTGTTGTTGATATTTGTAATGTTGAAGCTTCTGGATTTGAGATTTTATCTGTGTTTCATCCCACTGATGAAGTTATAAACTCTGTTGTTATTGCAAGGAAGAAAGTAGTGTTTGATCGAAACACGGTCGACGAGAACGACGAGAAGCGAGGTATTTTAGTTAATACCTCCTCTATTGTGTTGGCTGAGAAATGCTCTGGGTTTAATGGCTTTAGTCCCATGATTGAAGAGCAACTTTCTTGA

Protein sequence

MASQLVCSDPDLSQRMIFHTTDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGAYLMLRSAHGARAFLYPVVDICNVEASGFEILSVFHPTDEVINSVVIARKKVVFDRNTVDENDEKRGILVNTSSIVLAEKCSGFNGFSPMIEEQLS
Homology
[Close]
BLAST of Lsi11G006350 vs. Swiss-Prot
Match: NAS4_ARATH (Probable nicotianamine synthase 4 OS=Arabidopsis thaliana GN=NAS4 PE=2 SV=1)

HSP 1 Score: 165.2 bits (417), Expect = 5.7e-40
Identity = 91/163 (55.83%), Postives = 123/163 (75.46%), Query Frame = 1 

Query: 1   MASQLVCSDPDLSQRMIFHTTDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHM 60
           +A++LV SDPDLSQRM FHT D+M+VT+ LK ++VVFLAALVG++++EK +V++HL KHM
Sbjct: 165 VAARLVSSDPDLSQRMFFHTVDIMDVTESLKGFDVVFLAALVGMDKKEKVKVVEHLEKHM 224

Query: 61  AAGAYLMLRSAHGARAFLYPVVDICNVEASGFEILSVFHPTDEVINSVVIARKKVVFDRN 120
           + GA LMLRSAHG RAFLYP+V+ C++E  GFE+LSV+HPTDEVINS+VI+R K+  D N
Sbjct: 225 SPGALLMLRSAHGPRAFLYPIVEPCDLE--GFEVLSVYHPTDEVINSIVISR-KLGEDAN 284

Query: 121 TV--DENDEKRGILVNTSSI--VLAEKCSGFNGFSPMIEEQLS 160
            V  D  D+   +  N S I  ++ +K S    F+   EEQL+
Sbjct: 285 GVVHDHIDQASDLACNCSKIHVIMNKKKSIIEEFAGANEEQLT 324

[Close]
BLAST of Lsi11G006350 vs. Swiss-Prot
Match: NAS3_ARATH (Nicotianamine synthase 3 OS=Arabidopsis thaliana GN=NAS3 PE=2 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 1.2e-37
Identity = 76/116 (65.52%), Postives = 99/116 (85.34%), Query Frame = 1 

Query: 1   MASQLVCSDPDLSQRMIFHTTDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHM 60
           +AS LV SDPD+SQRM FHT D+M+VT+ LK ++VVFLAALVG+ +EEK +V++HL KHM
Sbjct: 164 LASLLVSSDPDISQRMFFHTVDIMDVTESLKSFDVVFLAALVGMNKEEKVKVIEHLQKHM 223

Query: 61  AAGAYLMLRSAHGARAFLYPVVDICNVEASGFEILSVFHPTDEVINSVVIARKKVV 117
           A GA LMLRSAHG RAFLYP+V+ C+++  GFE+LS++HPTD+VINSVVI++K  V
Sbjct: 224 APGAVLMLRSAHGPRAFLYPIVEPCDLQ--GFEVLSIYHPTDDVINSVVISKKHPV 277

[Close]
BLAST of Lsi11G006350 vs. Swiss-Prot
Match: NAS_SOLLC (Nicotianamine synthase OS=Solanum lycopersicum GN=CHLN PE=2 SV=1)

HSP 1 Score: 154.8 bits (390), Expect = 7.7e-37
Identity = 78/113 (69.03%), Postives = 96/113 (84.96%), Query Frame = 1 

Query: 1   MASQLVCSDPDLSQRMIFHTTDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHM 60
           MAS LV +DPD+S RM FHT DVM+VT  LKDY+VVFLAALVG+++E+K +V+ HL K+M
Sbjct: 162 MASALVAADPDMSSRMTFHTADVMDVTCALKDYDVVFLAALVGMDKEDKVKVVDHLAKYM 221

Query: 61  AAGAYLMLRSAHGARAFLYPVVDICNVEASGFEILSVFHPTDEVINSVVIARK 114
           + GA LMLRSAHGARAFLYPV+D  ++   GFE+LSV+HPTDEVINSV+IARK
Sbjct: 222 SPGATLMLRSAHGARAFLYPVLDPRDLR--GFEVLSVYHPTDEVINSVIIARK 272

[Close]
BLAST of Lsi11G006350 vs. Swiss-Prot
Match: NAS1_ARATH (Nicotianamine synthase 1 OS=Arabidopsis thaliana GN=NAS1 PE=2 SV=1)

HSP 1 Score: 149.4 bits (376), Expect = 3.2e-35
Identity = 72/113 (63.72%), Postives = 93/113 (82.30%), Query Frame = 1 

Query: 1   MASQLVCSDPDLSQRMIFHTTDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHM 60
           +AS LV  DPDLS+RMIFHTTDV+  T+GL  Y+VVFLAALVG+++E K + ++HL KHM
Sbjct: 165 LASNLVSRDPDLSKRMIFHTTDVLNATEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHM 224

Query: 61  AAGAYLMLRSAHGARAFLYPVVDICNVEASGFEILSVFHPTDEVINSVVIARK 114
           A GA LMLRSAH  RAFLYP+VD  + +  GF++L+++HPTD+V+NSVVIARK
Sbjct: 225 APGAVLMLRSAHALRAFLYPIVD--SSDLKGFQLLTIYHPTDDVVNSVVIARK 275

[Close]
BLAST of Lsi11G006350 vs. Swiss-Prot
Match: NAS2_ARATH (Nicotianamine synthase 2 OS=Arabidopsis thaliana GN=NAS2 PE=2 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 4.2e-35
Identity = 71/113 (62.83%), Postives = 94/113 (83.19%), Query Frame = 1 

Query: 1   MASQLVCSDPDLSQRMIFHTTDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHM 60
           +AS LV  D DLS+RMIFHTTDV+   +GL  Y+VVFLAALVG+++E K + ++HL KHM
Sbjct: 164 LASNLVSRDSDLSKRMIFHTTDVLNAKEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHM 223

Query: 61  AAGAYLMLRSAHGARAFLYPVVDICNVEASGFEILSVFHPTDEVINSVVIARK 114
           A GA +MLRSAHG RAFLYP+VD C+++  GFE+L+++HP+D+V+NSVVIARK
Sbjct: 224 APGAVVMLRSAHGLRAFLYPIVDSCDLK--GFEVLTIYHPSDDVVNSVVIARK 274

[Close]
BLAST of Lsi11G006350 vs. TrEMBL
Match: A0A0A0LGI4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G034530 PE=4 SV=1)

HSP 1 Score: 280.8 bits (717), Expect = 1.0e-72
Identity = 144/161 (89.44%), Postives = 153/161 (95.03%), Query Frame = 1 

Query: 1   MASQLVCSDPDLSQRMIFHTTDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHM 60
           MASQLVCSDPDLSQRMIFHT DVMEVTKGLKDYEVVFLAALVGL EEEKGRVLKHLGKHM
Sbjct: 169 MASQLVCSDPDLSQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLGEEEKGRVLKHLGKHM 228

Query: 61  AAGAYLMLRSAHGARAFLYPVVDICNVEASGFEILSVFHPTDEVINSVVIARKKVVFDRN 120
           AAG+YLMLRSAHGARAFLYPVVDIC VEASGFEILSVFHPTDEVINSVVIARKKV+FD++
Sbjct: 229 AAGSYLMLRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVMFDQS 288

Query: 121 T--VDENDEKRGILVNTSSIVLAEKCSGFNGFSPMIEEQLS 160
           +   ++ DEK+GILVNTSSIVLAEKCSGFNGF+PMIEE LS
Sbjct: 289 SDEDEDEDEKQGILVNTSSIVLAEKCSGFNGFTPMIEEPLS 329

[Close]
BLAST of Lsi11G006350 vs. TrEMBL
Match: M5VZ49_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa008576mg PE=4 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 3.6e-41
Identity = 89/150 (59.33%), Postives = 117/150 (78.00%), Query Frame = 1 

Query: 5   LVCSDPDLSQRMIFHTTDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGA 64
           LV SDPDLS+RM+FHTTD+M+VT  LKDY+VVFLAALVG+++ EK +++ HL K+MA GA
Sbjct: 165 LVSSDPDLSKRMVFHTTDIMDVTNALKDYDVVFLAALVGMDKMEKLKIIDHLAKYMAPGA 224

Query: 65  YLMLRSAHGARAFLYPVVDICNVEASGFEILSVFHPTDEVINSVVIARKKVVFDRNTVDE 124
            LMLRSAHGARAFLYPV+D C+ +  GFE+LSVFHPTDEVINSVVIARK  +  +++   
Sbjct: 225 TLMLRSAHGARAFLYPVIDPCD-DLRGFEVLSVFHPTDEVINSVVIARKYALPAQHSSPI 284

Query: 125 NDEKRGILVNTSSIVLAEKCSGFNGFSPMI 155
           + +     +N + I+L  KCSG   F+P++
Sbjct: 285 DSQG----LNAAPIILPNKCSGIEAFNPLL 309

[Close]
BLAST of Lsi11G006350 vs. TrEMBL
Match: A0A165CPN3_9ROSA (Nicotianamine synthase 2 OS=Pyrus betulifolia GN=Nas2 PE=2 SV=1)

HSP 1 Score: 174.5 bits (441), Expect = 1.0e-40
Identity = 94/162 (58.02%), Postives = 119/162 (73.46%), Query Frame = 1 

Query: 1   MASQLVCSDPDLSQRMIFHTTDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHM 60
           MA  LV SDPDLS+RM+FHTTD+M+VT  LKDYEVVFLAALVG+++ EK +++ HL K+M
Sbjct: 161 MALGLVSSDPDLSKRMVFHTTDIMDVTTALKDYEVVFLAALVGMDKMEKLKIIDHLAKYM 220

Query: 61  AAGAYLMLRSAHGARAFLYPVVDICNVEASGFEILSVFHPTDEVINSVVIARKKVVFDRN 120
           A GA LMLRSAHGARAFLYPV+D C+ +  GFE+LSVFHPTDEVINS+VIARK  +  ++
Sbjct: 221 APGATLMLRSAHGARAFLYPVIDPCD-DLRGFEVLSVFHPTDEVINSIVIARKYALPVQH 280

Query: 121 TVDENDEKRGILVNTSSIVLAEKCSGFNGFSP------MIEE 157
           +   N ++    +N  +I L  KC     F+P      MIEE
Sbjct: 281 SSSINSQE----INAGAIRLPSKCCEIEAFNPFQKLGSMIEE 317

[Close]
BLAST of Lsi11G006350 vs. TrEMBL
Match: A0A151TW62_CAJCA (Nicotianamine synthase OS=Cajanus cajan GN=KK1_010551 PE=4 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 2.3e-40
Identity = 93/167 (55.69%), Postives = 125/167 (74.85%), Query Frame = 1 

Query: 2   ASQLVCSDPDLSQRMIFHTTDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMA 61
           A++LV SDPDLS+RM+FHTTD+++V+ GLK+YEVV+LAALVG+++E K R++ HL K+MA
Sbjct: 162 ATRLVSSDPDLSKRMVFHTTDILDVSNGLKEYEVVYLAALVGMDKEGKNRIIDHLAKNMA 221

Query: 62  AGAYLMLRSAHGARAFLYPVVDICNVEASGFEILSVFHPTDEVINSVVIARKKVVFDRNT 121
            GA LMLRSAHGARAFLYPVV+ C++   GFE+LSVFHPTDEVINSVVIARK   + +  
Sbjct: 222 PGALLMLRSAHGARAFLYPVVEPCDLR--GFEVLSVFHPTDEVINSVVIARK---YPQPV 281

Query: 122 VDENDEKRGILVNTSSIVLAEKCSGFNGFSPM----------IEEQL 159
              + +++G+    +S++L  KCS    F+P+          IEEQL
Sbjct: 282 PMHSLDQQGL----ASMILPNKCSEIQAFNPLNHGNIIEELAIEEQL 319

[Close]
BLAST of Lsi11G006350 vs. TrEMBL
Match: M4FAP9_BRARP (Uncharacterized protein OS=Brassica rapa subsp. pekinensis PE=4 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 3.0e-40
Identity = 93/161 (57.76%), Postives = 124/161 (77.02%), Query Frame = 1 

Query: 1   MASQLVCSDPDLSQRMIFHTTDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHM 60
           +A++LV SDPDLSQRM FHT DVM+VT+ LKD++VVFLAALVG++++EK +V++HL KHM
Sbjct: 166 VAARLVSSDPDLSQRMFFHTVDVMDVTESLKDFDVVFLAALVGMDKQEKIKVIEHLEKHM 225

Query: 61  AAGAYLMLRSAHGARAFLYPVVDICNVEASGFEILSVFHPTDEVINSVVIARKKVVFDRN 120
           + GA LMLRSAHG RAFLYP+V+ C+++  GF++LSV+HPTDEVINSVVI+R K+V D  
Sbjct: 226 SPGALLMLRSAHGPRAFLYPIVEPCDLQ--GFQVLSVYHPTDEVINSVVISR-KLVGDVG 285

Query: 121 TVDENDEKRGILVNTSSI--VLAEKCSGFNGFSPMIEEQLS 160
             D  D+   +  N S I  ++ +K S    F+  IEEQLS
Sbjct: 286 NHDHMDQASELACNCSQIHAIMNKKKSIVEEFTGSIEEQLS 323

[Close]
BLAST of Lsi11G006350 vs. TAIR10
Match: AT1G56430.1 (AT1G56430.1 nicotianamine synthase 4)

HSP 1 Score: 165.2 bits (417), Expect = 3.2e-41
Identity = 91/163 (55.83%), Postives = 123/163 (75.46%), Query Frame = 1 

Query: 1   MASQLVCSDPDLSQRMIFHTTDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHM 60
           +A++LV SDPDLSQRM FHT D+M+VT+ LK ++VVFLAALVG++++EK +V++HL KHM
Sbjct: 165 VAARLVSSDPDLSQRMFFHTVDIMDVTESLKGFDVVFLAALVGMDKKEKVKVVEHLEKHM 224

Query: 61  AAGAYLMLRSAHGARAFLYPVVDICNVEASGFEILSVFHPTDEVINSVVIARKKVVFDRN 120
           + GA LMLRSAHG RAFLYP+V+ C++E  GFE+LSV+HPTDEVINS+VI+R K+  D N
Sbjct: 225 SPGALLMLRSAHGPRAFLYPIVEPCDLE--GFEVLSVYHPTDEVINSIVISR-KLGEDAN 284

Query: 121 TV--DENDEKRGILVNTSSI--VLAEKCSGFNGFSPMIEEQLS 160
            V  D  D+   +  N S I  ++ +K S    F+   EEQL+
Sbjct: 285 GVVHDHIDQASDLACNCSKIHVIMNKKKSIIEEFAGANEEQLT 324

[Close]
BLAST of Lsi11G006350 vs. TAIR10
Match: AT1G09240.1 (AT1G09240.1 nicotianamine synthase 3)

HSP 1 Score: 157.5 bits (397), Expect = 6.7e-39
Identity = 76/116 (65.52%), Postives = 99/116 (85.34%), Query Frame = 1 

Query: 1   MASQLVCSDPDLSQRMIFHTTDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHM 60
           +AS LV SDPD+SQRM FHT D+M+VT+ LK ++VVFLAALVG+ +EEK +V++HL KHM
Sbjct: 164 LASLLVSSDPDISQRMFFHTVDIMDVTESLKSFDVVFLAALVGMNKEEKVKVIEHLQKHM 223

Query: 61  AAGAYLMLRSAHGARAFLYPVVDICNVEASGFEILSVFHPTDEVINSVVIARKKVV 117
           A GA LMLRSAHG RAFLYP+V+ C+++  GFE+LS++HPTD+VINSVVI++K  V
Sbjct: 224 APGAVLMLRSAHGPRAFLYPIVEPCDLQ--GFEVLSIYHPTDDVINSVVISKKHPV 277

[Close]
BLAST of Lsi11G006350 vs. TAIR10
Match: AT5G04950.1 (AT5G04950.1 nicotianamine synthase 1)

HSP 1 Score: 149.4 bits (376), Expect = 1.8e-36
Identity = 72/113 (63.72%), Postives = 93/113 (82.30%), Query Frame = 1 

Query: 1   MASQLVCSDPDLSQRMIFHTTDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHM 60
           +AS LV  DPDLS+RMIFHTTDV+  T+GL  Y+VVFLAALVG+++E K + ++HL KHM
Sbjct: 165 LASNLVSRDPDLSKRMIFHTTDVLNATEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHM 224

Query: 61  AAGAYLMLRSAHGARAFLYPVVDICNVEASGFEILSVFHPTDEVINSVVIARK 114
           A GA LMLRSAH  RAFLYP+VD  + +  GF++L+++HPTD+V+NSVVIARK
Sbjct: 225 APGAVLMLRSAHALRAFLYPIVD--SSDLKGFQLLTIYHPTDDVVNSVVIARK 275

[Close]
BLAST of Lsi11G006350 vs. TAIR10
Match: AT5G56080.1 (AT5G56080.1 nicotianamine synthase 2)

HSP 1 Score: 149.1 bits (375), Expect = 2.4e-36
Identity = 71/113 (62.83%), Postives = 94/113 (83.19%), Query Frame = 1 

Query: 1   MASQLVCSDPDLSQRMIFHTTDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHM 60
           +AS LV  D DLS+RMIFHTTDV+   +GL  Y+VVFLAALVG+++E K + ++HL KHM
Sbjct: 164 LASNLVSRDSDLSKRMIFHTTDVLNAKEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHM 223

Query: 61  AAGAYLMLRSAHGARAFLYPVVDICNVEASGFEILSVFHPTDEVINSVVIARK 114
           A GA +MLRSAHG RAFLYP+VD C+++  GFE+L+++HP+D+V+NSVVIARK
Sbjct: 224 APGAVVMLRSAHGLRAFLYPIVDSCDLK--GFEVLTIYHPSDDVVNSVVIARK 274

[Close]
BLAST of Lsi11G006350 vs. TAIR10
Match: AT4G26483.1 (AT4G26483.1 nicotianamine synthases)

HSP 1 Score: 56.6 bits (135), Expect = 1.6e-08
Identity = 30/56 (53.57%), Postives = 41/56 (73.21%), Query Frame = 1 

Query: 60  MAAGAYLMLRSAHGARAFLYPVVDICNVEASGFEILSVFHP--TDEVINSVVIARK 114
           M  GA LMLRSA G R+FLY  VD C+++  GFE+L ++HP  +D  +NSV++ARK
Sbjct: 1   MDPGALLMLRSARGLRSFLYVDVDPCDLK--GFEVLEIYHPSMSDGFVNSVMVARK 54

[Close]
BLAST of Lsi11G006350 vs. NCBI nr
Match: gi|449454012|ref|XP_004144750.1| (PREDICTED: probable nicotianamine synthase 4 [Cucumis sativus])

HSP 1 Score: 280.8 bits (717), Expect = 1.5e-72
Identity = 144/161 (89.44%), Postives = 153/161 (95.03%), Query Frame = 1 

Query: 1   MASQLVCSDPDLSQRMIFHTTDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHM 60
           MASQLVCSDPDLSQRMIFHT DVMEVTKGLKDYEVVFLAALVGL EEEKGRVLKHLGKHM
Sbjct: 169 MASQLVCSDPDLSQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLGEEEKGRVLKHLGKHM 228

Query: 61  AAGAYLMLRSAHGARAFLYPVVDICNVEASGFEILSVFHPTDEVINSVVIARKKVVFDRN 120
           AAG+YLMLRSAHGARAFLYPVVDIC VEASGFEILSVFHPTDEVINSVVIARKKV+FD++
Sbjct: 229 AAGSYLMLRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVMFDQS 288

Query: 121 T--VDENDEKRGILVNTSSIVLAEKCSGFNGFSPMIEEQLS 160
           +   ++ DEK+GILVNTSSIVLAEKCSGFNGF+PMIEE LS
Sbjct: 289 SDEDEDEDEKQGILVNTSSIVLAEKCSGFNGFTPMIEEPLS 329

[Close]
BLAST of Lsi11G006350 vs. NCBI nr
Match: gi|659070114|ref|XP_008453482.1| (PREDICTED: probable nicotianamine synthase 4 isoform X2 [Cucumis melo])

HSP 1 Score: 226.9 bits (577), Expect = 2.5e-56
Identity = 115/118 (97.46%), Postives = 116/118 (98.31%), Query Frame = 1 

Query: 1   MASQLVCSDPDLSQRMIFHTTDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHM 60
           MASQLVCSDPDLSQRMIFHT DVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHM
Sbjct: 174 MASQLVCSDPDLSQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHM 233

Query: 61  AAGAYLMLRSAHGARAFLYPVVDICNVEASGFEILSVFHPTDEVINSVVIARKKVVFD 119
           AAG+YLMLRSAHGARAFLYPVVDIC VEASGFEILSVFHPTDEVINSVVIARKKVVFD
Sbjct: 234 AAGSYLMLRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVVFD 291

[Close]
BLAST of Lsi11G006350 vs. NCBI nr
Match: gi|659070112|ref|XP_008453473.1| (PREDICTED: probable nicotianamine synthase 4 isoform X1 [Cucumis melo])

HSP 1 Score: 226.9 bits (577), Expect = 2.5e-56
Identity = 115/118 (97.46%), Postives = 116/118 (98.31%), Query Frame = 1 

Query: 1   MASQLVCSDPDLSQRMIFHTTDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHM 60
           MASQLVCSDPDLSQRMIFHT DVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHM
Sbjct: 176 MASQLVCSDPDLSQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHM 235

Query: 61  AAGAYLMLRSAHGARAFLYPVVDICNVEASGFEILSVFHPTDEVINSVVIARKKVVFD 119
           AAG+YLMLRSAHGARAFLYPVVDIC VEASGFEILSVFHPTDEVINSVVIARKKVVFD
Sbjct: 236 AAGSYLMLRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVVFD 293

[Close]
BLAST of Lsi11G006350 vs. NCBI nr
Match: gi|720010607|ref|XP_010259301.1| (PREDICTED: nicotianamine synthase-like [Nelumbo nucifera])

HSP 1 Score: 176.4 bits (446), Expect = 3.9e-41
Identity = 97/160 (60.62%), Postives = 118/160 (73.75%), Query Frame = 1 

Query: 2   ASQLVCSDPDLSQRMIFHTTDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMA 61
           A +LV SDPDLSQRM FHTTD+M V+  L+DY+VVFLAALVGL+++EKGR++ HL KHMA
Sbjct: 164 ALRLVSSDPDLSQRMFFHTTDIMNVSSSLQDYDVVFLAALVGLDKDEKGRIIDHLAKHMA 223

Query: 62  AGAYLMLRSAHGARAFLYPVVDICNVEASGFEILSVFHPTDEVINSVVIARKKVVFDRNT 121
            GA LMLRSAHGARAFLYPVVD C++   GFE+LSVFHPTDEVINSVVIARK      + 
Sbjct: 224 PGALLMLRSAHGARAFLYPVVDPCHLR--GFEVLSVFHPTDEVINSVVIARK------HP 283

Query: 122 VDENDEKRGILVNTSSIVLAEKCSGFNGFSP-----MIEE 157
              +  ++G+    S ++L  KCS    F+P     MIEE
Sbjct: 284 KPTHALEQGL--GLSPMILPSKCSEIQAFNPLNHGSMIEE 313

[Close]
BLAST of Lsi11G006350 vs. NCBI nr
Match: gi|595826239|ref|XP_007205538.1| (hypothetical protein PRUPE_ppa008576mg [Prunus persica])

HSP 1 Score: 176.0 bits (445), Expect = 5.1e-41
Identity = 89/150 (59.33%), Postives = 117/150 (78.00%), Query Frame = 1 

Query: 5   LVCSDPDLSQRMIFHTTDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGA 64
           LV SDPDLS+RM+FHTTD+M+VT  LKDY+VVFLAALVG+++ EK +++ HL K+MA GA
Sbjct: 165 LVSSDPDLSKRMVFHTTDIMDVTNALKDYDVVFLAALVGMDKMEKLKIIDHLAKYMAPGA 224

Query: 65  YLMLRSAHGARAFLYPVVDICNVEASGFEILSVFHPTDEVINSVVIARKKVVFDRNTVDE 124
            LMLRSAHGARAFLYPV+D C+ +  GFE+LSVFHPTDEVINSVVIARK  +  +++   
Sbjct: 225 TLMLRSAHGARAFLYPVIDPCD-DLRGFEVLSVFHPTDEVINSVVIARKYALPAQHSSPI 284

Query: 125 NDEKRGILVNTSSIVLAEKCSGFNGFSPMI 155
           + +     +N + I+L  KCSG   F+P++
Sbjct: 285 DSQG----LNAAPIILPNKCSGIEAFNPLL 309

The following BLAST results are available for this feature:
BLAST of Lsi11G006350 vs. Swiss-Prot
Match NameE-valueIdentityDescription
NAS4_ARATH5.7e-4055.83Probable nicotianamine synthase 4 OS=Arabidopsis thaliana GN=NAS4 PE=2 SV=1[more]
NAS3_ARATH1.2e-3765.52Nicotianamine synthase 3 OS=Arabidopsis thaliana GN=NAS3 PE=2 SV=1[more]
NAS_SOLLC7.7e-3769.03Nicotianamine synthase OS=Solanum lycopersicum GN=CHLN PE=2 SV=1[more]
NAS1_ARATH3.2e-3563.72Nicotianamine synthase 1 OS=Arabidopsis thaliana GN=NAS1 PE=2 SV=1[more]
NAS2_ARATH4.2e-3562.83Nicotianamine synthase 2 OS=Arabidopsis thaliana GN=NAS2 PE=2 SV=1[more]
BLAST of Lsi11G006350 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LGI4_CUCSA1.0e-7289.44Uncharacterized protein OS=Cucumis sativus GN=Csa_2G034530 PE=4 SV=1[more]
M5VZ49_PRUPE3.6e-4159.33Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa008576mg PE=4 SV=1[more]
A0A165CPN3_9ROSA1.0e-4058.02Nicotianamine synthase 2 OS=Pyrus betulifolia GN=Nas2 PE=2 SV=1[more]
A0A151TW62_CAJCA2.3e-4055.69Nicotianamine synthase OS=Cajanus cajan GN=KK1_010551 PE=4 SV=1[more]
M4FAP9_BRARP3.0e-4057.76Uncharacterized protein OS=Brassica rapa subsp. pekinensis PE=4 SV=1[more]
BLAST of Lsi11G006350 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G56430.13.2e-4155.83 nicotianamine synthase 4[more]
AT1G09240.16.7e-3965.52 nicotianamine synthase 3[more]
AT5G04950.11.8e-3663.72 nicotianamine synthase 1[more]
AT5G56080.12.4e-3662.83 nicotianamine synthase 2[more]
AT4G26483.11.6e-0853.57 nicotianamine synthases[more]
BLAST of Lsi11G006350 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449454012|ref|XP_004144750.1|1.5e-7289.44PREDICTED: probable nicotianamine synthase 4 [Cucumis sativus][more]
gi|659070114|ref|XP_008453482.1|2.5e-5697.46PREDICTED: probable nicotianamine synthase 4 isoform X2 [Cucumis melo][more]
gi|659070112|ref|XP_008453473.1|2.5e-5697.46PREDICTED: probable nicotianamine synthase 4 isoform X1 [Cucumis melo][more]
gi|720010607|ref|XP_010259301.1|3.9e-4160.63PREDICTED: nicotianamine synthase-like [Nelumbo nucifera][more]
gi|595826239|ref|XP_007205538.1|5.1e-4159.33hypothetical protein PRUPE_ppa008576mg [Prunus persica][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0030418nicotianamine biosynthetic process
Vocabulary: Molecular Function
TermDefinition
GO:0030410nicotianamine synthase activity
Vocabulary: INTERPRO
TermDefinition
IPR004298Nicotian_synth
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030418 nicotianamine biosynthetic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0030410 nicotianamine synthase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi11G006350.1Lsi11G006350.1mRNA


InterPro
Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004298Nicotianamine synthasePFAMPF03059NAScoord: 2..115
score: 4.1
IPR004298Nicotianamine synthasePROFILEPS51142NAScoord: 1..118
score: 23
NoneNo IPR availablePANTHERPTHR32266FAMILY NOT NAMEDcoord: 1..157
score: 9.3
NoneNo IPR availablePANTHERPTHR32266:SF12NICOTIANAMINE SYNTHASE-RELATEDcoord: 1..157
score: 9.3

Synteny
The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Lsi11G006350Cucumber (Chinese Long) v3cuclsiB108
Lsi11G006350Watermelon (97103) v2lsiwmbB087
Lsi11G006350Watermelon (97103) v2lsiwmbB099
Lsi11G006350Wax gourdlsiwgoB101
Lsi11G006350Bottle gourd (USVL1VR-Ls)lsilsiB002
Lsi11G006350Bottle gourd (USVL1VR-Ls)lsilsiB060
Lsi11G006350Cucumber (Gy14) v1cgylsiB100
Lsi11G006350Cucurbita maxima (Rimu)cmalsiB159
Lsi11G006350Cucurbita maxima (Rimu)cmalsiB344
Lsi11G006350Cucurbita maxima (Rimu)cmalsiB715
Lsi11G006350Cucurbita maxima (Rimu)cmalsiB821
Lsi11G006350Cucurbita moschata (Rifu)cmolsiB034
Lsi11G006350Cucurbita moschata (Rifu)cmolsiB148
Lsi11G006350Cucurbita moschata (Rifu)cmolsiB331
Lsi11G006350Cucurbita moschata (Rifu)cmolsiB706
Lsi11G006350Cucurbita moschata (Rifu)cmolsiB808
Lsi11G006350Cucurbita pepo (Zucchini)cpelsiB075
Lsi11G006350Cucurbita pepo (Zucchini)cpelsiB659
Lsi11G006350Wild cucumber (PI 183967)cpilsiB096
Lsi11G006350Cucumber (Chinese Long) v2culsiB098
Lsi11G006350Melon (DHL92) v3.5.1lsimeB105
Lsi11G006350Melon (DHL92) v3.5.1lsimeB115
Lsi11G006350Watermelon (Charleston Gray)lsiwcgB105
Lsi11G006350Watermelon (Charleston Gray)lsiwcgB098
Lsi11G006350Watermelon (Charleston Gray)lsiwcgB111
Lsi11G006350Watermelon (97103) v1lsiwmB094
Lsi11G006350Watermelon (97103) v1lsiwmB107
Lsi11G006350Watermelon (97103) v1lsiwmB125
Lsi11G006350Cucumber (Gy14) v2cgyblsiB012
Lsi11G006350Cucumber (Gy14) v2cgyblsiB089
Lsi11G006350Melon (DHL92) v3.6.1lsimedB112
Lsi11G006350Melon (DHL92) v3.6.1lsimedB123
Lsi11G006350Silver-seed gourdcarlsiB062
Lsi11G006350Silver-seed gourdcarlsiB510
Lsi11G006350Silver-seed gourdcarlsiB801