Lsi11G000350 (gene) Bottle gourd (USVL1VR-Ls)

NameLsi11G000350
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionPolygalacturonase
Locationchr11 : 359118 .. 360671 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GCACACAAACAATGGGATTGAGATCAAACTTATTTGTGCCTTTACTATCTTTGTTGTTCTTGTTGGCATCGACGGTTAAAGGTCAATCTAATGGAGGTGTTTTTGATGTTACTTAGTATGGTGTCAAACCTAATGCTGATATTACCGAGGTAAGTATTTAGAAGTTTGATCTATAATCATTTTAATTTATAATCAAAACTTTTATATCTAATTCAATTTATAAGCTTTAGCAAATGCATGGAAGGAAGCATGTGCATCAACCACTCCAAGTAAAGTATTGATTCCAAAAGGAGTTTACCAATTAAGTCAATCTAACCTTAAAGGCCCTTGCAAGAGTTCAATTGAAGTTCAAGTCGAAGGGACATTACAAGCTCCTATTGACCCAAAAGGCGATGGATTGGTCATTTTAGAATATATTGATCAATTGACACTTTCAGGCACTGGAGTTTTTGATGGTCAAGGAAAAGCGAGCTGGGAAAAGAATGATTGCCACTAAAAAAAAATGTGTACTAAGCTTCCCATGGTAAACACTATGTTTATAATAGCTTTTTGTTTTTGGCCTAATTACATACATCCTTATTTAAACACATATCATGAATCACTTATTCACATATATACGTTTTTAATTCTTAAAATTTATTTTTATTTAAAATGTGCAGAATTTGAAGTTCAATTTTGTCACCAATTCAATAGTAAAGGATATAACTTCATTGGATAGTAAGAATTTCCATATATCTTTTGGGCTGTAAGAATCTTACATTTCAACATGTGACAATTACCGCACCAGAGAATAGCCCAAACACTGACGGAATTCACCTCAGTAGTTCAGAACAAATTAACATTCTCAATACAAAAATATCAACTGGAGATGATTGTGTGTCCGTAGGAGATAGTAACAAACAAATAACAATTAGTGATGTAACTTGTGGACCGGGACATGGCATCAGCATAGGCAGTTTGGGCAAGTACACCAAGGAACAAGCTGTGGAAGGAGTTACAGTGACAAAATGCAAATTAATCAGTACAACAAACGGTGTTAGGATCAAAACATGATCAGATTCTGCTTGCAAATATTCAGCCTCTGACATGCATTTTGAAGATATTGAAATGGTTAATGTTAGCAATCCTGTCATTATAGACCAAGAATATTGTCCATGGAATCAATGCAATCGAAAGGTATATATTAGAATTATGCCTAATTCATTTTTAACCCATAGATAACTTCAAGTATAAGAAAAAGTGTATATATTTATGTTAATAATATTTTTTTAACTATACATTTAGATTCCATCAAGTATTAAAATCAACAAAGTTAGTTTTAAAAATATTAGAGGCACTTCTGCTACTTCAGTCGCAGTCAAACTTGTTTGCAGCAAAAATATACCATGTGAAGGTGTGGAAGTTGCTGATATTGACCTCACATACCACGAAAATCAAGGACCAATTACATCTCAATGTGCAAATGTATCCTTTCTGGAAAGCAAAACCCTCGGACATGTGCTAAAGTTGCCCCTATTGATGCTCCATCAACAGACTAATTTTTTTTTACCATTA

mRNA sequence

GCACACAAACAATGGGATTGAGATCAAACTTATTTGTGCCTTTACTATCTTTGTTGTTCTTGTTGGCATCGACGGTTAAAGGTCAATCTAATGGAGCTTTAGCAAATGCATGGAAGGAAGCATGTGCATCAACCACTCCAAGTAAAGTATTGATTCCAAAAGGAGTTTACCAATTAAGTCAATCTAACCTTAAAGGCCCTTGCAAGAGTTCAATTGAAGTTCAAGTCGAAGGGACATTACAAGCTCCTATTGACCCAAAAGGCGATGGATTGGTCATTTTAGAATATATTGATCAATTGACACTTTCAGGCACTGGAGTTTTTGATGAATTTGAAGTTCAATTTTGTCACCAATTCAATATAAGAATTTCCATATATCTTTTGGGCTGTAAGAATCTTACATTTCAACATGTGACAATTACCGCACCAGAGAATAGCCCAAACACTGACGGAATTCACCTCAGTAGTTCAGAACAAATTAACATTCTCAATACAAAAATATCAACTGGAGATGATTGTGTGTCCGTAGGAGATAGTAACAAACAAATAACAATTAGTGATGTAACTTGTGGACCGGGACATGGCATCAGCATAGGCAGTTTGGGCAAGTACACCAAGGAACAAGCTGTGGAAGGAGTTACAGTGACAAAATGCAAATTAATCAGTACAACAAACGATTCTGCTTGCAAATATTCAGCCTCTGACATGCATTTTGAAGATATTGAAATGGTTAATGTTAGCAATCCTGTCATTATAGACCAAGAATATTGTCCATGGAATCAATGCAATCGAAAGATTCCATCAAGTATTAAAATCAACAAAGTTAGTTTTAAAAATATTAGAGGCACTTCTGCTACTTCAGTCGCAGTCAAACTTGTTTGCAGCAAAAATATACCATGTGAAGGTGTGGAAGTTGCTGATATTGACCTCACATACCACGAAAATCAAGGACCAATTACATCTCAATGTGCAAATGTATCCTTTCTGGAAAGCAAAACCCTCGGACATGTGCTAAAGTTGCCCCTATTGATGCTCCATCAACAGACTAATTTTTTTTTACCATTA

Coding sequence (CDS)

ATGGGATTGAGATCAAACTTATTTGTGCCTTTACTATCTTTGTTGTTCTTGTTGGCATCGACGGTTAAAGGTCAATCTAATGGAGCTTTAGCAAATGCATGGAAGGAAGCATGTGCATCAACCACTCCAAGTAAAGTATTGATTCCAAAAGGAGTTTACCAATTAAGTCAATCTAACCTTAAAGGCCCTTGCAAGAGTTCAATTGAAGTTCAAGTCGAAGGGACATTACAAGCTCCTATTGACCCAAAAGGCGATGGATTGGTCATTTTAGAATATATTGATCAATTGACACTTTCAGGCACTGGAGTTTTTGATGAATTTGAAGTTCAATTTTGTCACCAATTCAATATAAGAATTTCCATATATCTTTTGGGCTGTAAGAATCTTACATTTCAACATGTGACAATTACCGCACCAGAGAATAGCCCAAACACTGACGGAATTCACCTCAGTAGTTCAGAACAAATTAACATTCTCAATACAAAAATATCAACTGGAGATGATTGTGTGTCCGTAGGAGATAGTAACAAACAAATAACAATTAGTGATGTAACTTGTGGACCGGGACATGGCATCAGCATAGGCAGTTTGGGCAAGTACACCAAGGAACAAGCTGTGGAAGGAGTTACAGTGACAAAATGCAAATTAATCAGTACAACAAACGATTCTGCTTGCAAATATTCAGCCTCTGACATGCATTTTGAAGATATTGAAATGGTTAATGTTAGCAATCCTGTCATTATAGACCAAGAATATTGTCCATGGAATCAATGCAATCGAAAGATTCCATCAAGTATTAAAATCAACAAAGTTAGTTTTAAAAATATTAGAGGCACTTCTGCTACTTCAGTCGCAGTCAAACTTGTTTGCAGCAAAAATATACCATGTGAAGGTGTGGAAGTTGCTGATATTGACCTCACATACCACGAAAATCAAGGACCAATTACATCTCAATGTGCAAATGTATCCTTTCTGGAAAGCAAAACCCTCGGACATGTGCTAAAGTTGCCCCTATTGATGCTCCATCAACAGACTAATTTTTTTTTACCATTA

Protein sequence

MGLRSNLFVPLLSLLFLLASTVKGQSNGALANAWKEACASTTPSKVLIPKGVYQLSQSNLKGPCKSSIEVQVEGTLQAPIDPKGDGLVILEYIDQLTLSGTGVFDEFEVQFCHQFNIRISIYLLGCKNLTFQHVTITAPENSPNTDGIHLSSSEQINILNTKISTGDDCVSVGDSNKQITISDVTCGPGHGISIGSLGKYTKEQAVEGVTVTKCKLISTTNDSACKYSASDMHFEDIEMVNVSNPVIIDQEYCPWNQCNRKIPSSIKINKVSFKNIRGTSATSVAVKLVCSKNIPCEGVEVADIDLTYHENQGPITSQCANVSFLESKTLGHVLKLPLLMLHQQTNFFLPL
BLAST of Lsi11G000350 vs. Swiss-Prot
Match: PGLR_MEDSA (Polygalacturonase OS=Medicago sativa PE=2 SV=1)

HSP 1 Score: 325.5 bits (833), Expect = 7.3e-88
Identity = 171/349 (49.00%), Postives = 220/349 (63.04%), Query Frame = 1

Query: 29  ALANAWKEACASTTPSKVLIPKGVYQLSQSNLKGPCKSSIEVQVEGTLQAPIDP---KG- 88
           AL +AW EACASTT +K++IP G YQL+   LKGPCK+ IE+QV+GT+QAP DP   KG 
Sbjct: 41  ALTSAWNEACASTTAAKIVIPAGTYQLNGIELKGPCKAPIELQVDGTIQAPADPSVIKGT 100

Query: 89  DGLVILEYIDQLTLSGTGVFD---------------------------EFEVQFCHQFNI 148
           +      Y+D LTLSG GVFD                            F   F +   +
Sbjct: 101 EQWFKFLYMDHLTLSGKGVFDGQGATVYKKAAPASAWSGKNSNSKVFMNFGFNFVNNSIV 160

Query: 149 R---------ISIYLLGCKNLTFQHVTITAPENSPNTDGIHLSSSEQINILNTKISTGDD 208
           R           + + GCKN+TF   TITAP +SPNTDGIH+  S  + ILNT I TGDD
Sbjct: 161 RGVTSKDSKNFHVMVFGCKNITFDGFTITAPGDSPNTDGIHMGKSTDVKILNTNIGTGDD 220

Query: 209 CVSVGDSNKQITISDVTCGPGHGISIGSLGKYTKEQAVEGVTVTKCKLISTTN------- 268
           CVS+GD +KQIT+  V CGPGHG+S+GSLGK+T E+ VEG+TV  C L +T N       
Sbjct: 221 CVSIGDGSKQITVQGVNCGPGHGLSVGSLGKFTTEENVEGITVKNCTLTATDNGVRIKTW 280

Query: 269 -DSACKYSASDMHFEDIEMVNVSNPVIIDQEYCPWNQCNRKIPSSIKINKVSFKNIRGTS 328
            D+    + SD+HFEDI M NV NPVIIDQEY PWNQC++K PS IK++K+SFKN++GTS
Sbjct: 281 PDAPGTITVSDIHFEDITMTNVKNPVIIDQEYYPWNQCSKKNPSKIKLSKISFKNVKGTS 340

Query: 329 ATSVAVKLVCSKNIPCEGVEVADIDLTYHENQGPITSQCANVSFLESKT 330
            T+  V L+CS  +PC+GVE+ ++DL +  N  P T++C NV  L + T
Sbjct: 341 GTAEGVVLICSSAVPCDGVELNNVDLKF--NGAPTTAKCTNVKPLVTGT 387

BLAST of Lsi11G000350 vs. Swiss-Prot
Match: PGLR_OENOR (Exopolygalacturonase (Fragment) OS=Oenothera organensis PE=2 SV=1)

HSP 1 Score: 298.1 bits (762), Expect = 1.2e-79
Identity = 160/343 (46.65%), Postives = 213/343 (62.10%), Query Frame = 1

Query: 26  SNGALANAWKEACASTTPSKVLIPKGVYQLSQSNLKGPCKSSIEVQVEGTLQAPIDP--- 85
           S  AL  AWKEACAS +PS +L+PKG + +    L+GPCKSSI +Q++GTL+AP DP   
Sbjct: 2   STQALTTAWKEACASASPSTILVPKGNFAVGLITLEGPCKSSIGLQLQGTLKAPADPSKI 61

Query: 86  KGDGLVILEYIDQLTLSGTGVFD-----EFEVQFCHQ---------FNIRI--------- 145
           KG G + L  ID LT+ G GVFD      +    CH+          N+R+         
Sbjct: 62  KGLGWINLNKIDLLTIFGGGVFDGQGKSAWVQNDCHKNGPICKTLSMNLRLYAVTNSILR 121

Query: 146 SIYLLGCKNLTFQHVTITAPEN-------------SPNTDGIHLSSSEQINILNTKISTG 205
            +  L  KN    HV +   +N             S NTDGIH+  S+ +NI+NT+I TG
Sbjct: 122 DVTTLDSKNF---HVNVIGCKNLTFERFKISAAETSINTDGIHIGRSDGVNIINTEIKTG 181

Query: 206 DDCVSVGDSNKQITISDVTCGPGHGISIGSLGKYTKEQAVEGVTVTKCKLISTTNDSACK 265
           DDC+S+GD +K I I+++TCGPGHGIS+GSLG+Y  E++V G+ V  C +  + N    K
Sbjct: 182 DDCISLGDGSKNINITNITCGPGHGISVGSLGRYKNEESVVGIYVKNCTITGSQNGVRIK 241

Query: 266 Y-------SASDMHFEDIEMVNVSNPVIIDQEYCPWNQCNRKIPSSIKINKVSFKNIRGT 323
                    AS+MHF+DI M +V  P++IDQ YCP+NQC  ++PSS+K++K+SFKNI+GT
Sbjct: 242 TWPKSEPGEASEMHFQDITMNSVGTPILIDQGYCPYNQCTAEVPSSVKLSKISFKNIKGT 301

BLAST of Lsi11G000350 vs. Swiss-Prot
Match: PGLR_GOSHI (Polygalacturonase OS=Gossypium hirsutum GN=G9 PE=2 SV=1)

HSP 1 Score: 288.9 bits (738), Expect = 7.5e-77
Identity = 160/382 (41.88%), Postives = 221/382 (57.85%), Query Frame = 1

Query: 11  LLSLLFLLASTVKGQSNGALA-----------------NAWKEACASTTPSKVLIPKGVY 70
           +L LLF+ AS V+  +   +A                 +AWKEACAS TPS V+IPKG Y
Sbjct: 13  VLLLLFISASKVQSDAFDVVAKFGAKADGKTDLSKPFLDAWKEACASVTPSTVVIPKGTY 72

Query: 71  QLSQSNLKGPCKSSIEVQVEGTLQAPIDP---KGDGLVILEYIDQLTLSGTGVFD----- 130
            LS+ NL+GPCK+ IE+ V+GT+QAP DP   K    V    ++   + G G+FD     
Sbjct: 73  LLSKVNLEGPCKAPIEINVQGTIQAPADPSAFKDPNWVRFYSVENFKMFGGGIFDGQGSI 132

Query: 131 EFEVQFCHQFNIR----ISIYLLGCKNLTFQHVT----------------IT-------A 190
            +E   C     R    ++I      N   Q +T                IT       A
Sbjct: 133 AYEKNTCENREFRSKLPVNIRFDFLTNALIQDITSKDSKLFHINVFACKNITLERLKIEA 192

Query: 191 PENSPNTDGIHLSSSEQINILNTKISTGDDCVSVGDSNKQITISDVTCGPGHGISIGSLG 250
           P+ SPNTDGIH+  SE +NI+ + I TGDDC+S+GD  K + I ++TCGPGHGISIGSLG
Sbjct: 193 PDESPNTDGIHMGKSEGVNIIASDIKTGDDCISIGDGTKNMVIKEITCGPGHGISIGSLG 252

Query: 251 KYTKEQAVEGVTVTKCKLISTTNDSACKY-------SASDMHFEDIEMVNVSNPVIIDQE 310
           K+  E+ VEG+ ++ C + +T+N +  K        + S++HFEDI M NVS+P++IDQ+
Sbjct: 253 KFQNEEPVEGIKISNCTITNTSNGARIKTWPGEHGGAVSEIHFEDITMNNVSSPILIDQQ 312

Query: 311 YCPWNQCNRKIPSSIKINKVSFKNIRGTSATSVAVKLVCSKNIPCEGVEVADIDLTYHEN 334
           YCPWN+C +   S +K++ +SFKNIRGTSA   A+K +CS + PC+ VE+ADID+  H  
Sbjct: 313 YCPWNKCKKNEESKVKLSNISFKNIRGTSALPEAIKFICSGSSPCQNVELADIDIK-HNG 372

BLAST of Lsi11G000350 vs. Swiss-Prot
Match: PGLR_GOSBA (Polygalacturonase OS=Gossypium barbadense GN=G9 PE=2 SV=1)

HSP 1 Score: 287.3 bits (734), Expect = 2.2e-76
Identity = 161/386 (41.71%), Postives = 224/386 (58.03%), Query Frame = 1

Query: 11  LLSLLFLLASTVKGQSNGALA-----------------NAWKEACASTTPSKVLIPKGVY 70
           +L LLF+ AS V+  +   +A                 +AWKEACAS TPS V+IPKG Y
Sbjct: 13  VLLLLFISASKVQPDAFDVVAKFGAKADGKTDLSKPFLDAWKEACASVTPSTVVIPKGTY 72

Query: 71  QLSQSNLKGPCKSSIEVQVEGTLQAPIDP---KGDGLVILEYIDQLTLSGTGVFD----- 130
            LS+ NL+GPCK+ IE+ V+GT+QAP DP   K    V    ++   + G G+FD     
Sbjct: 73  LLSKVNLEGPCKAPIEINVQGTIQAPADPSAFKDPNWVRFYSVENFKMFGGGIFDGQGSI 132

Query: 131 EFEVQFCHQFNIR----ISIYLLGCKNLTFQHVT----------------IT-------A 190
            +E   C     R    ++I      N   Q +T                IT       A
Sbjct: 133 AYEKNTCENREFRSKLPVNIRFDFVTNALIQDITSKDSKLFHINVFACKNITLERLKIEA 192

Query: 191 PENSPNTDGIHLSSSEQINILNTKISTGDDCVSVGDSNKQITISDVTCGPGHGISIGSLG 250
           P+ SPNTDGIH+  SE +NI+ + I TGDDC+S+GD  K + I ++TCGPGHGISIGSLG
Sbjct: 193 PDESPNTDGIHMGKSEGVNIIASDIKTGDDCISIGDGTKNMVIKEITCGPGHGISIGSLG 252

Query: 251 KYTKEQAVEGVTVTKCKLISTTNDSACKY-------SASDMHFEDIEMVNVSNPVIIDQE 310
           K+  E+ VEG+ ++ C + +T+N +  K        + S++HFEDI M NVS+P++IDQ+
Sbjct: 253 KFQNEEPVEGIKISNCTITNTSNGARIKTWPGEHGGAVSEIHFEDITMNNVSSPILIDQQ 312

Query: 311 YCPWNQCNRKIPSSIKINKVSFKNIRGTSATSVAVKLVCSKNIPCEGVEVADIDLTYHEN 338
           YCPWN+C +   S +K++ +SFKNIRGTSA   A+K +CS + PC+ VE+ADID+  H  
Sbjct: 313 YCPWNKCKKNEESKVKLSNISFKNIRGTSALPEAIKFICSGSSPCQNVELADIDI-QHNG 372

BLAST of Lsi11G000350 vs. Swiss-Prot
Match: PGLR_TOBAC (Polygalacturonase OS=Nicotiana tabacum GN=PG1 PE=2 SV=1)

HSP 1 Score: 282.0 bits (720), Expect = 9.2e-75
Identity = 154/336 (45.83%), Postives = 209/336 (62.20%), Query Frame = 1

Query: 29  ALANAWKEACASTTPSKVLIPKGVYQLSQSNLKGPCKSSIEVQVEGTLQAPIDPK----G 88
           AL NA+KEAC ST+PS ++IPKG + ++Q  L+GPCKS +E+Q++ TL+AP DP     G
Sbjct: 43  ALLNAFKEACQSTSPSTIVIPKGTFTMNQVKLEGPCKSPLELQIQATLKAPSDPSQLKVG 102

Query: 89  DGLVILEYIDQLTLSGTGVFDE--------FEVQFCHQFNIRISIYLLGCKNLTFQHVTI 148
           + L +   +DQ T+SG G+ D          + + C++    +S   L   N T + +T 
Sbjct: 103 EWLTV-NKLDQFTMSGGGILDGQAAAAWECKQSKKCNKLPNNLSFNSL--TNSTIKDITT 162

Query: 149 -----------------------TAPENSPNTDGIHLSSSEQINILNTKISTGDDCVSVG 208
                                  +AP NSPNTDGIH+S S  +NI ++  STGDDC+SVG
Sbjct: 163 LDSKSFHVNVNQCKNLTFIRFNVSAPANSPNTDGIHVSRSSSVNITDSNFSTGDDCISVG 222

Query: 209 DSNKQITISDVTCGPGHGISIGSLGKYTKEQAVEGVTVTKCKLISTTNDSACK-YSAS-- 268
           D  +Q+ I+ VTCGPGHGIS+GSLG    E+ V GV V  C   +T N    K + AS  
Sbjct: 223 DETEQLYITRVTCGPGHGISVGSLGGNPDEKPVVGVFVRNCTFTNTDNGVRIKTWPASHP 282

Query: 269 ----DMHFEDIEMVNVSNPVIIDQEYCPWNQCNRKIPSSIKINKVSFKNIRGTSATSVAV 323
               D+HFEDI + NVSNPV+IDQ YCP+N+CN+ +PS +KI+KVSF+NI+GTS T  AV
Sbjct: 283 GVVNDVHFEDIIVQNVSNPVVIDQVYCPFNKCNKDLPSQVKISKVSFQNIKGTSRTQDAV 342

BLAST of Lsi11G000350 vs. TrEMBL
Match: E3VSV7_CUCPE (Polygalacturonase OS=Cucurbita pepo GN=PG1 PE=2 SV=1)

HSP 1 Score: 419.9 bits (1078), Expect = 3.1e-114
Identity = 227/380 (59.74%), Postives = 263/380 (69.21%), Query Frame = 1

Query: 1   MGLRSNLFVPLLSLLFLLASTVKGQSNG-----------------ALANAWKEACASTTP 60
           MGL S     +  LL L+++   G ++G                 ALA+AWKEACAS TP
Sbjct: 11  MGLLSKFMALIPLLLLLVSNATVGATSGVFDITTFGAKPNADATQALADAWKEACASATP 70

Query: 61  SKVLIPKGVYQLSQSNLKGPCKSSIEVQVEGTLQA-PIDPKGDGLVILEYIDQLTLSGTG 120
           SKVL+PKG YQL +S LKGPCKS IE+ VEGTLQA P D +GDGL+++EYID LTLSGTG
Sbjct: 71  SKVLVPKGTYQLGESRLKGPCKSPIELLVEGTLQASPTDTEGDGLLVMEYIDHLTLSGTG 130

Query: 121 VFDE-----FEVQFCH------------QFNI---------------RISIYLLGCKNLT 180
           VFD      +E   CH            +FN                   I +L  KNLT
Sbjct: 131 VFDGQGKAGWEKNDCHLKKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLT 190

Query: 181 FQHVTITAPENSPNTDGIHLSSSEQINILNTKISTGDDCVSVGDSNKQITISDVTCGPGH 240
           F H+ ITAP NSPNTDGIHL  SE + ILNT I+TGDDC+SVG +N++ITISDVTCGPGH
Sbjct: 191 FDHLKITAPHNSPNTDGIHLGDSEDVYILNTAIATGDDCISVGYTNRKITISDVTCGPGH 250

Query: 241 GISIGSLGKYTKEQAVEGVTVTKCKLISTTN--------DSACKYSASDMHFEDIEMVNV 300
           GISIGSLGKY  E+ V GVTV KCKLI TTN        DSA  Y ASDMHFEDIEM NV
Sbjct: 251 GISIGSLGKYKTEKEVVGVTVKKCKLIGTTNGVRIKSWPDSAVSYPASDMHFEDIEMENV 310

Query: 301 SNPVIIDQEYCPWNQCNRKIPSSIKINKVSFKNIRGTSATSVAVKLVCSKNIPCEGVEVA 323
           +NP+IIDQEYCPWNQCNRK+PS+IKI+KVSFKNIRGTS+T VAVKLVCSK+ PCEGVE+A
Sbjct: 311 ANPIIIDQEYCPWNQCNRKVPSAIKISKVSFKNIRGTSSTPVAVKLVCSKSNPCEGVEIA 370

BLAST of Lsi11G000350 vs. TrEMBL
Match: A0A147BN67_IXORI (Putative polygalacturonase/glycoside hydrolase family protein (Fragment) OS=Ixodes ricinus PE=3 SV=1)

HSP 1 Score: 351.3 bits (900), Expect = 1.4e-93
Identity = 188/338 (55.62%), Postives = 226/338 (66.86%), Query Frame = 1

Query: 29  ALANAWKEACASTTPSKVLIPKGVYQLSQSNLKGPCKSSIEVQVEGTLQAPIDP---KG- 88
           AL+NAWK+ACA+   SKV++P G Y+L   +LKGPCK  IEVQVEGTLQAP+DP   KG 
Sbjct: 19  ALSNAWKDACAAIGSSKVVVPAGTYKLGLVSLKGPCKGPIEVQVEGTLQAPVDPGYFKGS 78

Query: 89  DGLVILEYIDQLTLSGTGVFD-----EFEVQFCHQ--------FNIRIS----------- 148
           DG V+ E IDQ TLSG+G FD      ++   C +         NIR +           
Sbjct: 79  DGWVVFERIDQFTLSGSGSFDGQGQMAWKQNDCSKTFNCKTLAVNIRFNFITNSLVRDIK 138

Query: 149 --------IYLLGCKNLTFQHVTITAPENSPNTDGIHLSSSEQINILNTKISTGDDCVSV 208
                   I LLGCKN TFQHVTITAP +SPNTDGIH+  S  INI +T I+TGDDC+S+
Sbjct: 139 SLNSKNFHINLLGCKNFTFQHVTITAPGDSPNTDGIHIGRSSGINITDTNIATGDDCISM 198

Query: 209 GDSNKQITISDVTCGPGHGISIGSLGKYTKEQAVEGVTVTKCKLISTTNDSACKY----- 268
           GD ++ +TI+ VTCGPGHGISIGSLGKYT E+ V G+ V  C L  T N    K      
Sbjct: 199 GDGSQDVTINKVTCGPGHGISIGSLGKYTNEEPVSGIKVIGCTLTDTANGVRIKTWPSNP 258

Query: 269 ---SASDMHFEDIEMVNVSNPVIIDQEYCPWNQCNRKIPSSIKINKVSFKNIRGTSATSV 323
              SA+DMHFEDI M NV+NP+IIDQ YCPWNQC  ++PS IK++ +SF+ IRGTS+T  
Sbjct: 259 TSGSATDMHFEDIIMNNVANPIIIDQVYCPWNQCKAQVPSKIKLSNMSFRGIRGTSSTRE 318

BLAST of Lsi11G000350 vs. TrEMBL
Match: A0A058ZUL9_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_L01230 PE=3 SV=1)

HSP 1 Score: 334.0 bits (855), Expect = 2.3e-88
Identity = 174/336 (51.79%), Postives = 225/336 (66.96%), Query Frame = 1

Query: 29  ALANAWKEACASTTPSKVLIPKGVYQLSQSNLKGPCKSSIEVQVEGTLQAPIDP---KGD 88
           AL +AWKEACASTTP+KVLIP G + L++  L GPCK+ +E +++GTL+AP DP   K D
Sbjct: 43  ALLSAWKEACASTTPAKVLIPGGTFALTEVALDGPCKAPVEFELQGTLKAPADPSLFKTD 102

Query: 89  GLVILEYIDQLTLSGTGVFD---------------------EFEVQFCHQFNIRI----- 148
           G V+ ++ID LT+SG G FD                        ++F    N  I     
Sbjct: 103 GWVVFQHIDGLTMSGGGTFDGQGKTAWGQNDCAKNTNCRTLPTNLRFNFVTNALIHDVTT 162

Query: 149 ------SIYLLGCKNLTFQHVTITAPENSPNTDGIHLSSSEQINILNTKISTGDDCVSVG 208
                  + +LG KN+TFQ+  + AP +S NTDGIH+  S+ +NIL++ I TGDDCVS+G
Sbjct: 163 LDSKYFQVNVLGAKNMTFQNFHVNAPADSINTDGIHIGRSDGVNILDSDIKTGDDCVSLG 222

Query: 209 DSNKQITISDVTCGPGHGISIGSLGKYTKEQAVEGVTVTKCKLISTTNDSACKY------ 268
           D ++QIT+  VTCGPGHGIS+GSLGKY  E+ VEGV VT C +I   N    K       
Sbjct: 223 DGSRQITVKGVTCGPGHGISVGSLGKYENEEPVEGVFVTNCTIIGADNGVRIKTWPASKP 282

Query: 269 -SASDMHFEDIEMVNVSNPVIIDQEYCPWNQCNRKIPSSIKINKVSFKNIRGTSATSVAV 323
            +ASDMHFEDI+M NV NPVI+DQ YCP+NQC   +PS IKI+KVSFK IRGTSA+ VAV
Sbjct: 283 GTASDMHFEDIQMDNVLNPVILDQGYCPFNQCKAGVPSLIKISKVSFKKIRGTSASQVAV 342

BLAST of Lsi11G000350 vs. TrEMBL
Match: A0A058ZZK4_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_K00666 PE=3 SV=1)

HSP 1 Score: 334.0 bits (855), Expect = 2.3e-88
Identity = 174/336 (51.79%), Postives = 225/336 (66.96%), Query Frame = 1

Query: 29  ALANAWKEACASTTPSKVLIPKGVYQLSQSNLKGPCKSSIEVQVEGTLQAPIDP---KGD 88
           AL +AWKEACASTTP+KVLIP G + L++  L GPCK+ +E +++GTL+AP DP   K D
Sbjct: 43  ALLSAWKEACASTTPAKVLIPGGTFALTEVALDGPCKAPVEFELQGTLKAPADPSLFKTD 102

Query: 89  GLVILEYIDQLTLSGTGVFD---------------------EFEVQFCHQFNIRI----- 148
           G V+ ++ID LT+SG G FD                        ++F    N  I     
Sbjct: 103 GWVVFQHIDGLTMSGGGTFDGQGKTAWGQNDCAKNTNCRTLPTNLRFNFVTNALIHDVTT 162

Query: 149 ------SIYLLGCKNLTFQHVTITAPENSPNTDGIHLSSSEQINILNTKISTGDDCVSVG 208
                  + +LG KN+TFQ+  + AP +S NTDGIH+  S+ +NIL++ I TGDDCVS+G
Sbjct: 163 LDSKNFQVNVLGAKNMTFQNFHVNAPADSINTDGIHIGRSDGVNILDSDIKTGDDCVSLG 222

Query: 209 DSNKQITISDVTCGPGHGISIGSLGKYTKEQAVEGVTVTKCKLISTTNDSACKY------ 268
           D ++QIT+  VTCGPGHGIS+GSLGKY  E+ VEGV VT C +I   N    K       
Sbjct: 223 DGSRQITVKGVTCGPGHGISVGSLGKYENEEPVEGVFVTNCTIIGADNGVRIKTWPASKP 282

Query: 269 -SASDMHFEDIEMVNVSNPVIIDQEYCPWNQCNRKIPSSIKINKVSFKNIRGTSATSVAV 323
            +ASDMHFEDI+M NV NPVI+DQ YCP+NQC   +PS IKI+KVSFK IRGTSA+ VAV
Sbjct: 283 GTASDMHFEDIQMDNVLNPVILDQGYCPFNQCKAGVPSLIKISKVSFKKIRGTSASQVAV 342

BLAST of Lsi11G000350 vs. TrEMBL
Match: A0A068U9R1_COFCA (Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00017706001 PE=3 SV=1)

HSP 1 Score: 330.5 bits (846), Expect = 2.5e-87
Identity = 175/336 (52.08%), Postives = 216/336 (64.29%), Query Frame = 1

Query: 30  LANAWKEACASTTPSKVLIPKGVYQLSQSNLKGPCKSSIEVQVEGTLQAPIDP----KGD 89
           LA+AWK+AC STTPS +LIPKG + L +++L GPCK+ +EVQ++GT++AP DP    K  
Sbjct: 46  LADAWKQACNSTTPSTILIPKGTFLLKEASLAGPCKAPVEVQIQGTVKAPEDPAQITKDK 105

Query: 90  GLVILEYIDQLTLSGTGVFD-----------------------EFEVQFCHQFNIR---- 149
             + + Y+DQLTLSG G  D                          + F +   IR    
Sbjct: 106 EWMSIIYVDQLTLSGGGTLDGQGAKAWTQNECRVKTECSKLPNTLSLNFVNNTVIRDLTS 165

Query: 150 -----ISIYLLGCKNLTFQHVTITAPENSPNTDGIHLSSSEQINILNTKISTGDDCVSVG 209
                  + L GC N+TFQH TI AP +SPNTDGIH+  S  + I ++ I TGDDC+S+G
Sbjct: 166 LNSKLFHVNLFGCNNITFQHFTIIAPGDSPNTDGIHIGHSTGVVITDSNIGTGDDCISIG 225

Query: 210 DSNKQITISDVTCGPGHGISIGSLGKYTKEQAVEGVTVTKCKLISTTNDSACKY------ 269
           D  KQ+ IS VTCGPGHGIS+GSLG+Y  E  VEG+ VT C +  T N    K       
Sbjct: 226 DGAKQVNISKVTCGPGHGISVGSLGRYDNELPVEGIFVTDCTISGTLNGVRVKSWPASKS 285

Query: 270 -SASDMHFEDIEMVNVSNPVIIDQEYCPWNQCNRKIPSSIKINKVSFKNIRGTSATSVAV 323
            SA++MHFE I M NVSNPVIIDQEYCP NQC    PSS+KI +VSFKNI GTSAT  AV
Sbjct: 286 GSATNMHFEGIIMQNVSNPVIIDQEYCPNNQCTNTAPSSVKIAQVSFKNITGTSATPAAV 345

BLAST of Lsi11G000350 vs. TAIR10
Match: AT3G07820.1 (AT3G07820.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 270.4 bits (690), Expect = 1.6e-72
Identity = 152/352 (43.18%), Postives = 208/352 (59.09%), Query Frame = 1

Query: 15  LFLLASTVKGQSNGALANAWKEACASTTPSKVLIPKGVYQLSQSNLKGPCKSSIEVQVEG 74
           +F + S+       AL  A+  AC S++PSKV+IPKG ++L +  ++GPCK+ IEV ++G
Sbjct: 24  VFTIGSSSGSDITQALLKAFTSACQSSSPSKVVIPKGEFKLGEIEMRGPCKAPIEVTLQG 83

Query: 75  TLQA---PIDPKGDGLVILEYIDQLTLSGTGVFD-----EFEVQFCHQ--------FNIR 134
           T++A    I  K +  V+   ID   L+G G FD      + V  CH+         +IR
Sbjct: 84  TVKADGNAIQGK-EKWVVFGNIDGFKLNGGGAFDGEGNAAWRVNNCHKTFECKKLPISIR 143

Query: 135 ISIYL------LGCKNLTFQHVTITAPEN---------SPN----TDGIHLSSSEQINIL 194
               L      +   +    H+ +   +N         +P     TDGIHL  S+ + IL
Sbjct: 144 FDFILNSEIRDISSIDAKNFHINVLGAKNMTMNNIKIVAPEDSPNTDGIHLGRSDGVKIL 203

Query: 195 NTKISTGDDCVSVGDSNKQITISDVTCGPGHGISIGSLGKYTKEQAVEGVTVTKCKLIST 254
           N+ ISTGDDC+SVGD  K + +  VTCGPGHGIS+GSLG+Y  EQ V G+ V  C L  T
Sbjct: 204 NSFISTGDDCISVGDGMKNLHVEKVTCGPGHGISVGSLGRYGHEQDVSGIKVINCTLQET 263

Query: 255 TN--------DSACKYSASDMHFEDIEMVNVSNPVIIDQEYCPWNQCNRKIPSSIKINKV 314
            N         +AC  +ASD+HFEDI + +VSNP++IDQEYCPWNQCN++  S+IK+  +
Sbjct: 264 DNGLRIKTWPSAACSTTASDIHFEDIILKDVSNPILIDQEYCPWNQCNKQKASTIKLVNI 323

Query: 315 SFKNIRGTSATSVAVKLVCSKNIPCEGVEVADIDLTYHENQGPITSQCANVS 324
           SFKNIRGTS    AVKL+CSK  PC+ VE+ DID+ Y+   GP T  C+NVS
Sbjct: 324 SFKNIRGTSGNKDAVKLLCSKGYPCQNVEIGDIDIKYNGADGPATFHCSNVS 374

BLAST of Lsi11G000350 vs. TAIR10
Match: AT5G48140.1 (AT5G48140.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 247.3 bits (630), Expect = 1.4e-65
Identity = 118/211 (55.92%), Postives = 151/211 (71.56%), Query Frame = 1

Query: 121 IYLLGCKNLTFQHVTITAPENSPNTDGIHLSSSEQINILNTKISTGDDCVSVGDSNKQIT 180
           I ++G KN+TF+ V I AP  SPNTDGIH+  S+ I I+N+ ISTGDDCVSVGD  K + 
Sbjct: 164 INVIGAKNVTFEDVKIIAPAESPNTDGIHVGRSDGIKIINSFISTGDDCVSVGDGMKNLL 223

Query: 181 ISDVTCGPGHGISIGSLGKYTKEQAVEGVTVTKCKLISTTN--------DSACKYSASDM 240
           +  VTCGPGHGISIGSLG+Y+ E+ V G+ +  C L  T N         +AC  +ASD+
Sbjct: 224 VERVTCGPGHGISIGSLGRYSHEENVSGIKIINCTLQETDNGLRIKTWPSAACTTTASDI 283

Query: 241 HFEDIEMVNVSNPVIIDQEYCPWNQCNRKIPSSIKINKVSFKNIRGTSATSVAVKLVCSK 300
           HFE+I + NVSNP++IDQEYCPWNQCN++ PS+IK+  +SFK IRGTS    AVKL+CSK
Sbjct: 284 HFENILLKNVSNPILIDQEYCPWNQCNKQKPSTIKLANISFKKIRGTSGNKDAVKLLCSK 343

Query: 301 NIPCEGVEVADIDLTYHENQGPITSQCANVS 324
             PC+ VEV D+++ Y    GP T QC+NVS
Sbjct: 344 GYPCQNVEVGDVNIQYTGADGPATFQCSNVS 374

BLAST of Lsi11G000350 vs. TAIR10
Match: AT3G07840.1 (AT3G07840.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 246.1 bits (627), Expect = 3.2e-65
Identity = 119/211 (56.40%), Postives = 150/211 (71.09%), Query Frame = 1

Query: 121 IYLLGCKNLTFQHVTITAPENSPNTDGIHLSSSEQINILNTKISTGDDCVSVGDSNKQIT 180
           I +LG KN+TF +V + AP  SPNTDGIHL  SE + ILN+KI+TGDDC+SVGD  K + 
Sbjct: 165 INVLGAKNMTFDNVKVIAPAESPNTDGIHLGRSEGVKILNSKIATGDDCISVGDGMKNLH 224

Query: 181 ISDVTCGPGHGISIGSLGKYTKEQAVEGVTVTKCKLISTTN--------DSACKYSASDM 240
           + +V CGPGHGIS+GSLG+Y  EQ V G+TV  C L  T N         +AC  +AS +
Sbjct: 225 VENVMCGPGHGISVGSLGRYVHEQDVTGITVVNCTLQGTDNGLRIKTWPSAACATTASGI 284

Query: 241 HFEDIEMVNVSNPVIIDQEYCPWNQCNRKIPSSIKINKVSFKNIRGTSATSVAVKLVCSK 300
           HFE+I + NVSNP++IDQEYCPWNQCN++ PS+IK+  +SFKNIRGTS    AVKL+CSK
Sbjct: 285 HFENIILNNVSNPILIDQEYCPWNQCNKQKPSTIKLVDISFKNIRGTSGNKDAVKLLCSK 344

Query: 301 NIPCEGVEVADIDLTYHENQGPITSQCANVS 324
             PC  VE+ +I+L Y    GP T  C+NVS
Sbjct: 345 AHPCANVEIGNINLEYKGADGPPTFMCSNVS 375

BLAST of Lsi11G000350 vs. TAIR10
Match: AT3G07830.1 (AT3G07830.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 237.7 bits (605), Expect = 1.1e-62
Identity = 114/210 (54.29%), Postives = 147/210 (70.00%), Query Frame = 1

Query: 121 IYLLGCKNLTFQHVTITAPENSPNTDGIHLSSSEQINILNTKISTGDDCVSVGDSNKQIT 180
           I ++G KN+TF +V I AP  SPNTDGIHL  S  ++I+N++ISTGDDCVSVGD    + 
Sbjct: 165 INVIGAKNMTFDNVKIMAPAESPNTDGIHLGRSVGVSIINSRISTGDDCVSVGDGMVNLL 224

Query: 181 ISDVTCGPGHGISIGSLGKYTKEQAVEGVTVTKCKLISTTN--------DSACKYSASDM 240
           + +V CGPGHGIS+GSLG+Y  EQ V G+ V  C L  T N         +AC  +AS++
Sbjct: 225 VKNVVCGPGHGISVGSLGRYGHEQDVSGIRVINCTLQETDNGLRIKTWPSAACSTTASNI 284

Query: 241 HFEDIEMVNVSNPVIIDQEYCPWNQCNRKIPSSIKINKVSFKNIRGTSATSVAVKLVCSK 300
           HFE+I + NVSNP++IDQEYCPWNQCN++  SSIK+  +SF+ IRGTS    AVKL+CSK
Sbjct: 285 HFENIILRNVSNPILIDQEYCPWNQCNKQKSSSIKLANISFRRIRGTSGNKDAVKLLCSK 344

Query: 301 NIPCEGVEVADIDLTYHENQGPITSQCANV 323
             PCE V+V DI++ Y    GP T  C+NV
Sbjct: 345 GYPCENVQVGDINIQYTGADGPATFMCSNV 374

BLAST of Lsi11G000350 vs. TAIR10
Match: AT3G14040.1 (AT3G14040.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 222.6 bits (566), Expect = 3.7e-58
Identity = 105/207 (50.72%), Postives = 142/207 (68.60%), Query Frame = 1

Query: 123 LLGCKNLTFQHVTITAPENSPNTDGIHLSSSEQINILNTKISTGDDCVSVGDSNKQITIS 182
           +L CKN+T   + I AP  S NTDGIH+  S  +N++  KI TGDDCVS+GD  + + + 
Sbjct: 218 ILNCKNITLTDIGIDAPPESLNTDGIHIGRSNGVNLIGAKIKTGDDCVSIGDGTENLIVE 277

Query: 183 DVTCGPGHGISIGSLGKYTKEQAVEGVTVTKCKLISTTNDSACKY-------SASDMHFE 242
           +V CGPGHGISIGSLG+Y  EQ V+GVTV KC + +T N    K         AS++ FE
Sbjct: 278 NVECGPGHGISIGSLGRYPNEQPVKGVTVRKCLIKNTDNGVRIKTWPGSPPGIASNILFE 337

Query: 243 DIEMVNVSNPVIIDQEYCPWNQCNRKIPSSIKINKVSFKNIRGTSATSVAVKLVCSKNIP 302
           DI M NVS PV+IDQEYCP+  C   +PS +K++ V+ KNI+GTSAT VAVKL+CSK +P
Sbjct: 338 DITMDNVSLPVLIDQEYCPYGHCKAGVPSKVKLSDVTIKNIKGTSATKVAVKLMCSKGVP 397

Query: 303 CEGVEVADIDLTYHENQGPITSQCANV 323
           C  + ++DI+L ++  +GP  S C+N+
Sbjct: 398 CTNIALSDINLVHNGKEGPAVSACSNI 424

BLAST of Lsi11G000350 vs. NCBI nr
Match: gi|659120497|ref|XP_008460220.1| (PREDICTED: exopolygalacturonase-like [Cucumis melo])

HSP 1 Score: 498.4 bits (1282), Expect = 1.0e-137
Identity = 272/380 (71.58%), Postives = 289/380 (76.05%), Query Frame = 1

Query: 1   MGLRSNLFVPLLSLLFLLASTVKGQSNG------------------ALANAWKEACASTT 60
           MGLRSN F P LSLLFLLAST KGQSNG                  AL NAWK ACAST+
Sbjct: 1   MGLRSN-FAPFLSLLFLLASTTKGQSNGGVFDVTRYGAKPNTDITHALLNAWKGACASTS 60

Query: 61  PSKVLIPKGVYQLSQSNLKGPCKSSIEVQVEGTLQAPIDPKGDGLVILEYIDQLTLSGTG 120
            SKVLIPKGVY LSQSNLKGPCKSSIE+QV+GTLQAP DPKGDGLVILEYID+LTLSGTG
Sbjct: 61  RSKVLIPKGVYLLSQSNLKGPCKSSIEIQVDGTLQAPPDPKGDGLVILEYIDKLTLSGTG 120

Query: 121 VFDE-----FEVQFCHQFNI---------------------------RISIYLLGCKNLT 180
           VFD      +E   CH+  I                              I +LGCKNLT
Sbjct: 121 VFDGQGKVGWEKNDCHKKKICTKLPMTLKFNFVTNSIVRDITSLDSKNFHINILGCKNLT 180

Query: 181 FQHVTITAPENSPNTDGIHLSSSEQINILNTKISTGDDCVSVGDSNKQITISDVTCGPGH 240
           FQHVTI+APE+SPNTDGIH+SSSEQI ILNTKISTGDDCVSVGDSNKQITI DVTCGPGH
Sbjct: 181 FQHVTISAPEDSPNTDGIHISSSEQITILNTKISTGDDCVSVGDSNKQITIRDVTCGPGH 240

Query: 241 GISIGSLGKYTKEQAVEGVTVTKCKLISTTN--------DSACKYSASDMHFEDIEMVNV 300
           GISIGSLGKY+KE+AVEGV VTKCKL STTN        DSA +YSASDMHFEDIEM NV
Sbjct: 241 GISIGSLGKYSKEKAVEGVWVTKCKLTSTTNGVRIKTWPDSAGEYSASDMHFEDIEMNNV 300

Query: 301 SNPVIIDQEYCPWNQCNRKIPSSIKINKVSFKNIRGTSATSVAVKLVCSKNIPCEGVEVA 323
           SNP+IIDQEYCPWNQCN KIPSSIKI+KVSFKNIRGTSAT VAVKLVCSK IPCE VEV 
Sbjct: 301 SNPIIIDQEYCPWNQCNPKIPSSIKISKVSFKNIRGTSATPVAVKLVCSKKIPCEAVEVV 360

BLAST of Lsi11G000350 vs. NCBI nr
Match: gi|449445367|ref|XP_004140444.1| (PREDICTED: exopolygalacturonase-like [Cucumis sativus])

HSP 1 Score: 495.0 bits (1273), Expect = 1.1e-136
Identity = 268/377 (71.09%), Postives = 288/377 (76.39%), Query Frame = 1

Query: 1   MGLRSNLFVPLLSLLFLLASTVKGQS---------------NGALANAWKEACASTTPSK 60
           MGLR N FV  LSLLFLLAST KGQS                 AL NAWK ACAST+ SK
Sbjct: 1   MGLRWN-FVAFLSLLFLLASTTKGQSVFDVTRYGAKPNTDITHALLNAWKGACASTSRSK 60

Query: 61  VLIPKGVYQLSQSNLKGPCKSSIEVQVEGTLQAPIDPKGDGLVILEYIDQLTLSGTGVFD 120
           VLIPKG+Y LSQSNLKGPCKSSIE+QV+GTLQAP DPKGDGLVILEYIDQLTLSGTGVFD
Sbjct: 61  VLIPKGIYLLSQSNLKGPCKSSIEIQVQGTLQAPPDPKGDGLVILEYIDQLTLSGTGVFD 120

Query: 121 E-----FEVQFCHQFNI---------------------------RISIYLLGCKNLTFQH 180
                 +E   CH+  I                              I LLGCKN+TF+H
Sbjct: 121 GQGKAGWEKNDCHKKKICTKLPMTLKFNFVTNSIVKDITSLDSKNFHINLLGCKNITFEH 180

Query: 181 VTITAPENSPNTDGIHLSSSEQINILNTKISTGDDCVSVGDSNKQITISDVTCGPGHGIS 240
           VTI+APENSPNTDGIH+SSSEQI ILNTKISTGDDCVSVGDSNK ITI DVTCGPGHGIS
Sbjct: 181 VTISAPENSPNTDGIHISSSEQIRILNTKISTGDDCVSVGDSNKDITIRDVTCGPGHGIS 240

Query: 241 IGSLGKYTKEQAVEGVTVTKCKLISTTN--------DSACKYSASDMHFEDIEMVNVSNP 300
           IGSLGKYTKE+AVEGV VTKCKLI TTN        DSA KYSASDMHFEDIEM++VSNP
Sbjct: 241 IGSLGKYTKEKAVEGVWVTKCKLIKTTNGVRIKTWPDSAVKYSASDMHFEDIEMIDVSNP 300

Query: 301 VIIDQEYCPWNQCNRKIPSSIKINKVSFKNIRGTSATSVAVKLVCSKNIPCEGVEVADID 323
           ++IDQEYCPWNQCNRKIPSSIKI+KVSFKNIRGT+ T VAVKLVCSK+IPCE VEVADID
Sbjct: 301 ILIDQEYCPWNQCNRKIPSSIKISKVSFKNIRGTTVTPVAVKLVCSKSIPCEAVEVADID 360

BLAST of Lsi11G000350 vs. NCBI nr
Match: gi|778658546|ref|XP_011652893.1| (PREDICTED: exopolygalacturonase-like [Cucumis sativus])

HSP 1 Score: 462.2 bits (1188), Expect = 7.9e-127
Identity = 252/382 (65.97%), Postives = 275/382 (71.99%), Query Frame = 1

Query: 1   MGLRSNLFVPLLSLLFLLASTVKGQS--------------------NGALANAWKEACAS 60
           MGLRSN FV LL L  LL S  KGQS                     G L  AWK+ACAS
Sbjct: 1   MGLRSN-FVTLLPLFLLLVSNAKGQSAAGGGVFDVTSFGAKPNGDITGPLLVAWKDACAS 60

Query: 61  TTPSKVLIPKGVYQLSQSNLKGPCKSSIEVQVEGTLQAPIDPKGDGLVILEYIDQLTLSG 120
           TTPSKVLIPKG Y LSQSNLKGPCKS IE+Q+EGTLQA  DPKGDGL++LEYIDQLT+SG
Sbjct: 61  TTPSKVLIPKGSYGLSQSNLKGPCKSPIELQIEGTLQASPDPKGDGLLLLEYIDQLTVSG 120

Query: 121 TGVFDE-----FEVQFCHQFNI---------------------------RISIYLLGCKN 180
           TGV D      +E   CH   I                              I LLGCKN
Sbjct: 121 TGVLDGQGKAGWEKNDCHLKKICTKLPMTLKLNFITNSIVKDITSLDSKNYHINLLGCKN 180

Query: 181 LTFQHVTITAPENSPNTDGIHLSSSEQINILNTKISTGDDCVSVGDSNKQITISDVTCGP 240
           LTF HVTITAPENSPNTDGIH+SSSEQINILNT I+TGDDC+SVGD+NKQI ISDVTCGP
Sbjct: 181 LTFDHVTITAPENSPNTDGIHVSSSEQINILNTNIATGDDCISVGDTNKQIVISDVTCGP 240

Query: 241 GHGISIGSLGKYTKEQAVEGVTVTKCKLISTTN--------DSACKYSASDMHFEDIEMV 300
           GHGISIGSLGKYTKE+ V GVTV KCKL STTN        DS   + A+DMHFEDIEM 
Sbjct: 241 GHGISIGSLGKYTKEKEVVGVTVKKCKLTSTTNGVRIKTWPDSVGTFPATDMHFEDIEMD 300

Query: 301 NVSNPVIIDQEYCPWNQCNRKIPSSIKINKVSFKNIRGTSATSVAVKLVCSKNIPCEGVE 323
           NVSNP+IIDQEYCPWNQCNRK+PS IKI+KVSFKNIRGTSAT+VAVK+VCS++ PCE VE
Sbjct: 301 NVSNPIIIDQEYCPWNQCNRKVPSKIKISKVSFKNIRGTSATAVAVKIVCSQSNPCEEVE 360

BLAST of Lsi11G000350 vs. NCBI nr
Match: gi|659067859|ref|XP_008441641.1| (PREDICTED: exopolygalacturonase-like [Cucumis melo])

HSP 1 Score: 462.2 bits (1188), Expect = 7.9e-127
Identity = 251/380 (66.05%), Postives = 279/380 (73.42%), Query Frame = 1

Query: 1   MGLRSNLFVPLLSLLFLLASTVKGQSNGA------------------LANAWKEACASTT 60
           MGLRSN FV LL L  LL S  K QS+GA                  L +AWKE CAS T
Sbjct: 1   MGLRSN-FVALLLLFLLLVSNSKAQSSGAVFDVTSFGAKPDADITGALVSAWKEGCASIT 60

Query: 61  PSKVLIPKGVYQLSQSNLKGPCKSSIEVQVEGTLQAPIDPKGDGLVILEYIDQLTLSGTG 120
           PSKVLIPKG Y LSQSNLKGPCKSSIE+Q+EGTLQAP DPKGDGL+ILEY+D+LT+SG G
Sbjct: 61  PSKVLIPKGSYGLSQSNLKGPCKSSIELQIEGTLQAPSDPKGDGLLILEYVDRLTVSGMG 120

Query: 121 VFDE-----FEVQFCH--------QFNIRIS-------------------IYLLGCKNLT 180
           V D      +E   CH          N++++                   I LLGCKNLT
Sbjct: 121 VLDGQGKEGWEKNDCHLKKICTKLPMNLKLNFITNSIVKDITSLDSKNYHINLLGCKNLT 180

Query: 181 FQHVTITAPENSPNTDGIHLSSSEQINILNTKISTGDDCVSVGDSNKQITISDVTCGPGH 240
           F HVTITAP +SPNTDGIH+SSSE+INILNT I+TGDDC+SVGD+NKQI ISDVTCGPGH
Sbjct: 181 FDHVTITAPGDSPNTDGIHVSSSEEINILNTNIATGDDCISVGDTNKQIVISDVTCGPGH 240

Query: 241 GISIGSLGKYTKEQAVEGVTVTKCKLISTTN--------DSACKYSASDMHFEDIEMVNV 300
           GISIGSLGKY+KE+ V GVTV KCKL STTN        DS   + ASDMHFEDIEMVNV
Sbjct: 241 GISIGSLGKYSKEKEVVGVTVKKCKLTSTTNGVRIKTWPDSVGTFPASDMHFEDIEMVNV 300

Query: 301 SNPVIIDQEYCPWNQCNRKIPSSIKINKVSFKNIRGTSATSVAVKLVCSKNIPCEGVEVA 323
           SNPVIIDQEYCPWNQCNRK+PS IKI+KVSFKNIRGTSAT VAVK+VCSK+ PCE VEVA
Sbjct: 301 SNPVIIDQEYCPWNQCNRKVPSKIKISKVSFKNIRGTSATPVAVKIVCSKSNPCEEVEVA 360

BLAST of Lsi11G000350 vs. NCBI nr
Match: gi|659134134|ref|XP_008467043.1| (PREDICTED: exopolygalacturonase-like, partial [Cucumis melo])

HSP 1 Score: 459.1 bits (1180), Expect = 6.7e-126
Identity = 236/334 (70.66%), Postives = 264/334 (79.04%), Query Frame = 1

Query: 29  ALANAWKEACASTTPSKVLIPKGVYQLSQSNLKGPCKSSIEVQVEGTLQAPIDPKGDGLV 88
           ALA+AWKEACASTTPSKVLIPKG Y LSQSNLKGPCKS+IE+Q+EGTLQAP DPKGDGL+
Sbjct: 7   ALASAWKEACASTTPSKVLIPKGSYGLSQSNLKGPCKSAIELQIEGTLQAPADPKGDGLL 66

Query: 89  ILEYIDQLTLSGTGVFDE-----FEVQFCH--------QFNIRIS--------------- 148
           +LEY+DQLT+SGTGV D      +E   CH          N++++               
Sbjct: 67  LLEYVDQLTVSGTGVLDGQGKAGWEKNDCHLKKECTKLPMNLKLNFVTNSIVKDITSLDS 126

Query: 149 ----IYLLGCKNLTFQHVTITAPENSPNTDGIHLSSSEQINILNTKISTGDDCVSVGDSN 208
               I LLGCKNLTF HVTITAP NSPNTDGIH+SSSEQINILNT I+TGDDC+SVGD+N
Sbjct: 127 KNYHINLLGCKNLTFDHVTITAPGNSPNTDGIHVSSSEQINILNTNIATGDDCISVGDTN 186

Query: 209 KQITISDVTCGPGHGISIGSLGKYTKEQAVEGVTVTKCKLISTTN--------DSACKYS 268
           KQ+ ISDVTCGPGHGISIGSLGKY+KE+ V GVTV KCKL  TTN        DSA  + 
Sbjct: 187 KQVVISDVTCGPGHGISIGSLGKYSKEKEVVGVTVKKCKLTDTTNGVRIKTWPDSAVAFP 246

Query: 269 ASDMHFEDIEMVNVSNPVIIDQEYCPWNQCNRKIPSSIKINKVSFKNIRGTSATSVAVKL 323
           ASDMHFEDIEMVNVSNPVIIDQEYCPWNQCNRK+PS IKI+ VSFKNIRGTSAT VAVK+
Sbjct: 247 ASDMHFEDIEMVNVSNPVIIDQEYCPWNQCNRKVPSKIKISNVSFKNIRGTSATPVAVKI 306

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PGLR_MEDSA7.3e-8849.00Polygalacturonase OS=Medicago sativa PE=2 SV=1[more]
PGLR_OENOR1.2e-7946.65Exopolygalacturonase (Fragment) OS=Oenothera organensis PE=2 SV=1[more]
PGLR_GOSHI7.5e-7741.88Polygalacturonase OS=Gossypium hirsutum GN=G9 PE=2 SV=1[more]
PGLR_GOSBA2.2e-7641.71Polygalacturonase OS=Gossypium barbadense GN=G9 PE=2 SV=1[more]
PGLR_TOBAC9.2e-7545.83Polygalacturonase OS=Nicotiana tabacum GN=PG1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
E3VSV7_CUCPE3.1e-11459.74Polygalacturonase OS=Cucurbita pepo GN=PG1 PE=2 SV=1[more]
A0A147BN67_IXORI1.4e-9355.62Putative polygalacturonase/glycoside hydrolase family protein (Fragment) OS=Ixod... [more]
A0A058ZUL9_EUCGR2.3e-8851.79Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_L01230 PE=3 SV=1[more]
A0A058ZZK4_EUCGR2.3e-8851.79Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_K00666 PE=3 SV=1[more]
A0A068U9R1_COFCA2.5e-8752.08Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00017706001 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G07820.11.6e-7243.18 Pectin lyase-like superfamily protein[more]
AT5G48140.11.4e-6555.92 Pectin lyase-like superfamily protein[more]
AT3G07840.13.2e-6556.40 Pectin lyase-like superfamily protein[more]
AT3G07830.11.1e-6254.29 Pectin lyase-like superfamily protein[more]
AT3G14040.13.7e-5850.72 Pectin lyase-like superfamily protein[more]
Match NameE-valueIdentityDescription
gi|659120497|ref|XP_008460220.1|1.0e-13771.58PREDICTED: exopolygalacturonase-like [Cucumis melo][more]
gi|449445367|ref|XP_004140444.1|1.1e-13671.09PREDICTED: exopolygalacturonase-like [Cucumis sativus][more]
gi|778658546|ref|XP_011652893.1|7.9e-12765.97PREDICTED: exopolygalacturonase-like [Cucumis sativus][more]
gi|659067859|ref|XP_008441641.1|7.9e-12766.05PREDICTED: exopolygalacturonase-like [Cucumis melo][more]
gi|659134134|ref|XP_008467043.1|6.7e-12670.66PREDICTED: exopolygalacturonase-like, partial [Cucumis melo][more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0005975carbohydrate metabolic process
Vocabulary: Molecular Function
TermDefinition
GO:0004650polygalacturonase activity
Vocabulary: INTERPRO
TermDefinition
IPR012334Pectin_lyas_fold
IPR011050Pectin_lyase_fold/virulence
IPR000743Glyco_hydro_28
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0071555 cell wall organization
cellular_component GO:0005575 cellular_component
cellular_component GO:0005576 extracellular region
molecular_function GO:0004650 polygalacturonase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi11G000350.1Lsi11G000350.1mRNA


Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 38..105
score: 1.4E-7coord: 121..323
score: 2.0
IPR000743Glycoside hydrolase, family 28PROSITEPS00502POLYGALACTURONASEcoord: 183..196
scor
IPR011050Pectin lyase fold/virulence factorunknownSSF51126Pectin lyase-likecoord: 28..323
score: 2.51
IPR012334Pectin lyase foldGENE3DG3DSA:2.160.20.10coord: 29..323
score: 2.0
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 29..323
score: 3.1E
NoneNo IPR availablePANTHERPTHR31375:SF37PECTIN LYASE-LIKE SUPERFAMILY PROTEIN-RELATEDcoord: 29..323
score: 3.1E

The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Lsi11G000350MELO3C022583.2Melon (DHL92) v3.6.1lsimedB088
Lsi11G000350Cla97C11G207070Watermelon (97103) v2lsiwmbB079
Lsi11G000350CsGy5G009350Cucumber (Gy14) v2cgyblsiB311
Lsi11G000350CsaV3_5G012460Cucumber (Chinese Long) v3cuclsiB363
The following gene(s) are paralogous to this gene:

None