Lsi10G008120 (gene) Bottle gourd (USVL1VR-Ls)

NameLsi10G008120
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionMechanosensitive ion channel protein 8
Locationchr10 : 11138221 .. 11141899 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCGGGTAATGCTTCGTTGAGAGGTATGAAGTTGGTGAACACCAGTGGGAAGATTTGGCAGGAGTCCAGCAATGATTTCTGGAATGATAGTGATAATAGACTAAATAATGGCGAAGTTACGAATGGTAATGGTGGTGGTGGTAGGAGTGAGGATTTTGAGCTTAGGCAACAAAGGAAGGATGTGGAAGATCCGCCGTCGAAGCTTATTGGACAGTTGCTTCACAAGCAGAAGGGGTCCGGAGAGATATCTTTAGATATGGATATGGAAATGCTGGAACTACCACAGGATAAGACTCTGCTTCCTATGGTCGCGGATTCTCCGATGCGAAGACGGCACAGAGACTCTCTGCCGGTTGATGAAGAACATAGAGGAAAGCAACCGCAGCAGCGTGATCTTCGAGCGCATGGCAGCAATGGTGAAGATGATGGTGGTGCTGAGGTTTTGAGATGCACATCTAATTCGTCCTTTGGAAGAGATGTGTCGTTCCAGAGGAAATCAGGGCAAGTTCGCCGTGGTTTACTGTCAAAAGCCATAGATGAGGAAGACGATGATCCATTCTTGGAAGAAGATGTTCCTGATAAGTATAAGAAGGGTAATCTAGGTGTTTTGACTCTGTTACAGTGGGCGAGTTTGATTTTGATAATCGCAGCATTGATTTGCACTTTGGCAATTCGTAACTGGAGGAGAAAGAAACTATGGAAGCTTGAACTATGGAAATGGGAGGTTATGATTCTTGTTCTGATATGTGGAAGGTTGGTTTCTGGCTGGGGGATTCGGATAATTGTCTTCTTCATTGAAAGAAACTTTCTACTGCGTAAGAGGGTTCTATACTTTGTTTATGGAATCAGAAAGGCAGTCCAGAATTGTCTTTGGTTGGGTCTAGTTTTAATTGCTTGGAACTTCCTGTTTGATGACAAAGTTCAAAGAGAGGTAAAGAGCAATGCCTTAGAATATGTGACCAAAGTATTGGTATGTCTTCTTGTAAGCACTTTGGTTTGGCTAGTAAAAACGCTAATGGTGAAGGTCTTGGCCTCTTTTTTCCATGTGAGTACATATTTTGATAGGATTCAAGCTGCATTGTTTGACCAATATGTAATTGGGACACTGTTGGGGCCTCCATTGATGGAGATTTCAAAGAATGAGGAAGAAGAAGAGAGGGTTGCAGAAGAGGTTATAAAGTTAGAGAATGCAGGGGCTACCATCCCTCCTGATTTAAAGGCAACAGCTCAAAAGGGTGGGAGGGTGATAGGGGAGGGTATAACAATTGATCACTTGCATAAGCTTAGTCCTAAGAATGTGTCTGCCTGGAATATGAAGAGGTTGATGAAAATCATTAGACATGTGACTCTTTCAACTTTGGATGAACAAATCAAAGATTCAATAACTCATGAGAACGAATCAACCACACAGATCAAGAGTGGATATGAGGCAAAAGTTGCTGCTAAGAAGATTTTTCTGAACGTGGCTAGGCATGGTTCTAAGTAAGTTAATTCATTTCATACAGCCTAATACTGCTATCGTTTGAATTGACTGTTTAAAAAGTGTTATTGTAAGTGAAAAAAAAAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCTTTTATTATTACTATGAAACGACTGGATAAATTGTGTTTTATGAAAAAATGTTCATTCAAAATTGTGGCTAGCTCCAATTAATGAACCTTTTACTCCATTCTTAATTTTCCCCCCTTTGTTAGATATATCTACGTAGAGGACTTGAAGCGTTTCATGGACGAAGATAAGGCTTCAAAAACCATGGGACTGTTTGAAGAAGCACGTGAGAGTAGGAAAATAAGCAAATCGTCATTGAAAAACTGGGTGGTAAGAGCTATCTTATCAAAGTCTTAAATATATTTTTATGTTCTCATTCCACATGTTATTGTTGCAAATTCAATATGGGTGTTGTCTTGATATTAAATTAGTGTTGGTCTAATGATATTATACCTAAAAATTAAAATTGTTGCAGATTTATGCCTCTAGGGAACGAACGGCTCTAGCTTCGACATTAGACGATTCCAAAACAGCTGTGAAAAAACTTCACCGCATGGTGAACATTTTAATTTCTGTCATTATTCTGGTTATTTGGCTATTGATTCTTGGTATAGCCACTAGCAAATTTCTCCTATTCGCGACCTCTCAACTTGTCCTTGTCGCATTTGTATTTGGAAATACTTGCAAGACTGTTTTTGAATCAATCATCTTCTTATTTGTAATGCATCCATTTGATGTTGGAAACCTATGCGAAATCAATGGTGTGCAGGTAATTCTTCTCTTCAACTAATTATTTTAATTCTACATTCCCATCTCATATTCTTTGATTTAAACTGTAGTTCCTTCAAATTTCAGATGATTGTAAAAGAAATGAACATTTTGACGACCATTTTTCTAAGATATGACAACCAGAAGATCATAATTCCGAACAGTCTTCTCGCGACAATGGCTATCCACAACTTCTATCGTAGTCCTGACATGGGAGATGCTGTAGAATTCTGTCTTCACATATGTACTCCGCCTGAGAAAATTGCTATTATGAGACAAAGAATTATAAGGTATGTGGAGAAAAGAAAACACAGTTTACTAAAAATTCATTTTATTATTGTTTTTTATTTTTTTTTTATTTTTTTTTGTCTTTGCAGTTACATTGAGGGCAAGAAAAAGCATTGGTGTCCTGCGCCAAGGATTTTCCTCAAGGATGTAGAAGAGTTAAACAGAATCAGAATAGCAATATGTCTTACTCATAGAATGAACCATCAAGACATGGGCAAGAGGTGGACTAGGAGAGCTCTTTTGGTCGAAGAACTCGTTAAAATTTTCCAAGAGATCGACTTGCAATACCGTCTCTCAGGGGGCTAG

mRNA sequence

ATGTCGGGTAATGCTTCGTTGAGAGGTATGAAGTTGGTGAACACCAGTGGGAAGATTTGGCAGGAGTCCAGCAATGATTTCTGGAATGATAGTGATAATAGACTAAATAATGGCGAAGTTACGAATGGTAATGGTGGTGGTGGTAGGAGTGAGGATTTTGAGCTTAGGCAACAAAGGAAGGATGTGGAAGATCCGCCGTCGAAGCTTATTGGACAGTTGCTTCACAAGCAGAAGGGGTCCGGAGAGATATCTTTAGATATGGATATGGAAATGCTGGAACTACCACAGGATAAGACTCTGCTTCCTATGGTCGCGGATTCTCCGATGCGAAGACGGCACAGAGACTCTCTGCCGGTTGATGAAGAACATAGAGGAAAGCAACCGCAGCAGCGTGATCTTCGAGCGCATGGCAGCAATGGTGAAGATGATGGTGGTGCTGAGGTTTTGAGATGCACATCTAATTCGTCCTTTGGAAGAGATGTGTCGTTCCAGAGGAAATCAGGGCAAGTTCGCCGTGGTTTACTGTCAAAAGCCATAGATGAGGAAGACGATGATCCATTCTTGGAAGAAGATGTTCCTGATAAGTATAAGAAGGGTAATCTAGGTGTTTTGACTCTGTTACAGTGGGCGAGTTTGATTTTGATAATCGCAGCATTGATTTGCACTTTGGCAATTCGTAACTGGAGGAGAAAGAAACTATGGAAGCTTGAACTATGGAAATGGGAGGTTATGATTCTTGTTCTGATATGTGGAAGGTTGGTTTCTGGCTGGGGGATTCGGATAATTGTCTTCTTCATTGAAAGAAACTTTCTACTGCGTAAGAGGGTTCTATACTTTGTTTATGGAATCAGAAAGGCAGTCCAGAATTGTCTTTGGTTGGGTCTAGTTTTAATTGCTTGGAACTTCCTGTTTGATGACAAAGTTCAAAGAGAGGTAAAGAGCAATGCCTTAGAATATGTGACCAAAGTATTGGTATGTCTTCTTGTAAGCACTTTGGTTTGGCTAGTAAAAACGCTAATGGTGAAGGTCTTGGCCTCTTTTTTCCATGTGAGTACATATTTTGATAGGATTCAAGCTGCATTGTTTGACCAATATGTAATTGGGACACTGTTGGGGCCTCCATTGATGGAGATTTCAAAGAATGAGGAAGAAGAAGAGAGGGTTGCAGAAGAGGTTATAAAGTTAGAGAATGCAGGGGCTACCATCCCTCCTGATTTAAAGGCAACAGCTCAAAAGGGTGGGAGGGTGATAGGGGAGGGTATAACAATTGATCACTTGCATAAGCTTAGTCCTAAGAATGTGTCTGCCTGGAATATGAAGAGGTTGATGAAAATCATTAGACATGTGACTCTTTCAACTTTGGATGAACAAATCAAAGATTCAATAACTCATGAGAACGAATCAACCACACAGATCAAGAGTGGATATGAGGCAAAAGTTGCTGCTAAGAAGATTTTTCTGAACGTGGCTAGGCATGGTTCTAAATATATCTACGTAGAGGACTTGAAGCGTTTCATGGACGAAGATAAGGCTTCAAAAACCATGGGACTGTTTGAAGAAGCACGTGAGAGTAGGAAAATAAGCAAATCGTCATTGAAAAACTGGGTGATTTATGCCTCTAGGGAACGAACGGCTCTAGCTTCGACATTAGACGATTCCAAAACAGCTGTGAAAAAACTTCACCGCATGGTGAACATTTTAATTTCTGTCATTATTCTGGTTATTTGGCTATTGATTCTTGGTATAGCCACTAGCAAATTTCTCCTATTCGCGACCTCTCAACTTGTCCTTGTCGCATTTGTATTTGGAAATACTTGCAAGACTGTTTTTGAATCAATCATCTTCTTATTTGTAATGCATCCATTTGATGTTGGAAACCTATGCGAAATCAATGGTGTGCAGATGATTGTAAAAGAAATGAACATTTTGACGACCATTTTTCTAAGATATGACAACCAGAAGATCATAATTCCGAACAGTCTTCTCGCGACAATGGCTATCCACAACTTCTATCGTAGTCCTGACATGGGAGATGCTGTAGAATTCTGTCTTCACATATGTACTCCGCCTGAGAAAATTGCTATTATGAGACAAAGAATTATAAGTTACATTGAGGGCAAGAAAAAGCATTGGTGTCCTGCGCCAAGGATTTTCCTCAAGGATGTAGAAGAGTTAAACAGAATCAGAATAGCAATATGTCTTACTCATAGAATGAACCATCAAGACATGGGCAAGAGGTGGACTAGGAGAGCTCTTTTGGTCGAAGAACTCGTTAAAATTTTCCAAGAGATCGACTTGCAATACCGTCTCTCAGGGGGCTAG

Coding sequence (CDS)

ATGTCGGGTAATGCTTCGTTGAGAGGTATGAAGTTGGTGAACACCAGTGGGAAGATTTGGCAGGAGTCCAGCAATGATTTCTGGAATGATAGTGATAATAGACTAAATAATGGCGAAGTTACGAATGGTAATGGTGGTGGTGGTAGGAGTGAGGATTTTGAGCTTAGGCAACAAAGGAAGGATGTGGAAGATCCGCCGTCGAAGCTTATTGGACAGTTGCTTCACAAGCAGAAGGGGTCCGGAGAGATATCTTTAGATATGGATATGGAAATGCTGGAACTACCACAGGATAAGACTCTGCTTCCTATGGTCGCGGATTCTCCGATGCGAAGACGGCACAGAGACTCTCTGCCGGTTGATGAAGAACATAGAGGAAAGCAACCGCAGCAGCGTGATCTTCGAGCGCATGGCAGCAATGGTGAAGATGATGGTGGTGCTGAGGTTTTGAGATGCACATCTAATTCGTCCTTTGGAAGAGATGTGTCGTTCCAGAGGAAATCAGGGCAAGTTCGCCGTGGTTTACTGTCAAAAGCCATAGATGAGGAAGACGATGATCCATTCTTGGAAGAAGATGTTCCTGATAAGTATAAGAAGGGTAATCTAGGTGTTTTGACTCTGTTACAGTGGGCGAGTTTGATTTTGATAATCGCAGCATTGATTTGCACTTTGGCAATTCGTAACTGGAGGAGAAAGAAACTATGGAAGCTTGAACTATGGAAATGGGAGGTTATGATTCTTGTTCTGATATGTGGAAGGTTGGTTTCTGGCTGGGGGATTCGGATAATTGTCTTCTTCATTGAAAGAAACTTTCTACTGCGTAAGAGGGTTCTATACTTTGTTTATGGAATCAGAAAGGCAGTCCAGAATTGTCTTTGGTTGGGTCTAGTTTTAATTGCTTGGAACTTCCTGTTTGATGACAAAGTTCAAAGAGAGGTAAAGAGCAATGCCTTAGAATATGTGACCAAAGTATTGGTATGTCTTCTTGTAAGCACTTTGGTTTGGCTAGTAAAAACGCTAATGGTGAAGGTCTTGGCCTCTTTTTTCCATGTGAGTACATATTTTGATAGGATTCAAGCTGCATTGTTTGACCAATATGTAATTGGGACACTGTTGGGGCCTCCATTGATGGAGATTTCAAAGAATGAGGAAGAAGAAGAGAGGGTTGCAGAAGAGGTTATAAAGTTAGAGAATGCAGGGGCTACCATCCCTCCTGATTTAAAGGCAACAGCTCAAAAGGGTGGGAGGGTGATAGGGGAGGGTATAACAATTGATCACTTGCATAAGCTTAGTCCTAAGAATGTGTCTGCCTGGAATATGAAGAGGTTGATGAAAATCATTAGACATGTGACTCTTTCAACTTTGGATGAACAAATCAAAGATTCAATAACTCATGAGAACGAATCAACCACACAGATCAAGAGTGGATATGAGGCAAAAGTTGCTGCTAAGAAGATTTTTCTGAACGTGGCTAGGCATGGTTCTAAATATATCTACGTAGAGGACTTGAAGCGTTTCATGGACGAAGATAAGGCTTCAAAAACCATGGGACTGTTTGAAGAAGCACGTGAGAGTAGGAAAATAAGCAAATCGTCATTGAAAAACTGGGTGATTTATGCCTCTAGGGAACGAACGGCTCTAGCTTCGACATTAGACGATTCCAAAACAGCTGTGAAAAAACTTCACCGCATGGTGAACATTTTAATTTCTGTCATTATTCTGGTTATTTGGCTATTGATTCTTGGTATAGCCACTAGCAAATTTCTCCTATTCGCGACCTCTCAACTTGTCCTTGTCGCATTTGTATTTGGAAATACTTGCAAGACTGTTTTTGAATCAATCATCTTCTTATTTGTAATGCATCCATTTGATGTTGGAAACCTATGCGAAATCAATGGTGTGCAGATGATTGTAAAAGAAATGAACATTTTGACGACCATTTTTCTAAGATATGACAACCAGAAGATCATAATTCCGAACAGTCTTCTCGCGACAATGGCTATCCACAACTTCTATCGTAGTCCTGACATGGGAGATGCTGTAGAATTCTGTCTTCACATATGTACTCCGCCTGAGAAAATTGCTATTATGAGACAAAGAATTATAAGTTACATTGAGGGCAAGAAAAAGCATTGGTGTCCTGCGCCAAGGATTTTCCTCAAGGATGTAGAAGAGTTAAACAGAATCAGAATAGCAATATGTCTTACTCATAGAATGAACCATCAAGACATGGGCAAGAGGTGGACTAGGAGAGCTCTTTTGGTCGAAGAACTCGTTAAAATTTTCCAAGAGATCGACTTGCAATACCGTCTCTCAGGGGGCTAG

Protein sequence

MSGNASLRGMKLVNTSGKIWQESSNDFWNDSDNRLNNGEVTNGNGGGGRSEDFELRQQRKDVEDPPSKLIGQLLHKQKGSGEISLDMDMEMLELPQDKTLLPMVADSPMRRRHRDSLPVDEEHRGKQPQQRDLRAHGSNGEDDGGAEVLRCTSNSSFGRDVSFQRKSGQVRRGLLSKAIDEEDDDPFLEEDVPDKYKKGNLGVLTLLQWASLILIIAALICTLAIRNWRRKKLWKLELWKWEVMILVLICGRLVSGWGIRIIVFFIERNFLLRKRVLYFVYGIRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLVSTLVWLVKTLMVKVLASFFHVSTYFDRIQAALFDQYVIGTLLGPPLMEISKNEEEEERVAEEVIKLENAGATIPPDLKATAQKGGRVIGEGITIDHLHKLSPKNVSAWNMKRLMKIIRHVTLSTLDEQIKDSITHENESTTQIKSGYEAKVAAKKIFLNVARHGSKYIYVEDLKRFMDEDKASKTMGLFEEARESRKISKSSLKNWVIYASRERTALASTLDDSKTAVKKLHRMVNILISVIILVIWLLILGIATSKFLLFATSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGNLCEINGVQMIVKEMNILTTIFLRYDNQKIIIPNSLLATMAIHNFYRSPDMGDAVEFCLHICTPPEKIAIMRQRIISYIEGKKKHWCPAPRIFLKDVEELNRIRIAICLTHRMNHQDMGKRWTRRALLVEELVKIFQEIDLQYRLSGG
BLAST of Lsi10G008120 vs. Swiss-Prot
Match: MSL6_ARATH (Mechanosensitive ion channel protein 6 OS=Arabidopsis thaliana GN=MSL6 PE=1 SV=1)

HSP 1 Score: 808.5 bits (2087), Expect = 6.2e-233
Identity = 451/818 (55.13%), Postives = 580/818 (70.90%), Query Frame = 1

Query: 15  TSGKIWQESSNDFWNDSDNRLNNGEVTNG---------NGGGGRSEDFELRQQRKDVEDP 74
           T GKIW++ S DFW D +  LN G               G   + E FE R+     EDP
Sbjct: 29  TVGKIWRDGSYDFWTDGEGNLNKGHNAAAVDSDRSAATTGEQQKDEGFEFRRG----EDP 88

Query: 75  PSKLIGQLLHKQKGSGEISLDMDMEMLELPQDKTLLPMVADSPMRRRHRDSLPVDEEHRG 134
           P+KLIGQ LHKQ+ SGEI LDMD+ M EL Q + L P V++SP     RD  PV      
Sbjct: 89  PTKLIGQFLHKQQASGEICLDMDLGMDEL-QSRGLTP-VSESPRVSTKRD--PVG----- 148

Query: 135 KQPQQRDLRAHGSNGEDDGGAEVLRCTSNSS-FGRDVSFQRK------------------ 194
               +RD R++ +N  DDG  EV++C+ N++   R  S   K                  
Sbjct: 149 ----RRDSRSN-TNNNDDG--EVVKCSGNNAPIQRSSSTLLKMRTRSRLSDPPTPQLPPQ 208

Query: 195 SGQVRRGLLSKAIDE---------------EDDDPFLEEDVPDKYKKGNLGVLTLLQWAS 254
           +  ++ G + K+                  E+DDPF  ED+P++Y+K  L +  +L+W S
Sbjct: 209 TADMKSGRIPKSGQMKSGFFGKSPKTQGEEEEDDPFAAEDLPEEYRKDKLSLWIVLEWLS 268

Query: 255 LILIIAALICTLAIRNWRRKKLWKLELWKWEVMILVLICGRLVSGWGIRIIVFFIERNFL 314
           LILIIA  +CTLAI + R+KKLW+L+LWKWE M+LVLICGRLVS W ++I+VFFIERNFL
Sbjct: 269 LILIIAGFVCTLAIPSLRKKKLWELQLWKWESMVLVLICGRLVSSWIVKIVVFFIERNFL 328

Query: 315 LRKRVLYFVYGIRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLVST 374
           LRKRVLYFVYG+RKAVQNCLWLGLVL+AW+FLFD+KV +   + AL  VTK+ VCLLV  
Sbjct: 329 LRKRVLYFVYGVRKAVQNCLWLGLVLLAWHFLFDEKVAKAANTKALRVVTKIFVCLLVGF 388

Query: 375 LVWLVKTLMVKVLASFFHVSTYFDRIQAALFDQYVIGTLLGPPLMEISKNEEEEERVAEE 434
           L+WLVKTL+VKVLAS FH+STYFDRIQ +LF QYVI TL GPPL+EI KNEEEEER++ E
Sbjct: 389 LLWLVKTLLVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLIEIQKNEEEEERISVE 448

Query: 435 VIKLENAGATIPPDLKATAQK-------------------GGRVIGEGITIDHLHKLSPK 494
           V K +N G     ++++ AQK                   GG    +GITID LHKL+PK
Sbjct: 449 VKKFQNPGGV---EIQSGAQKSPMKTGKSPFLSHVLSNGGGGGGENKGITIDSLHKLNPK 508

Query: 495 NVSAWNMKRLMKIIRHVTLSTLDEQIKDSITHENESTTQIKSGYEAKVAAKKIFLNVARH 554
           NVSAW MKRLM IIR+ +L+TLDEQ++D  + +++   QI+S +EAK+AA+KIF NVA+ 
Sbjct: 509 NVSAWKMKRLMNIIRNGSLTTLDEQLQDP-SLDDDKGNQIRSEFEAKLAARKIFHNVAKP 568

Query: 555 GSKYIYVEDLKRFMDEDKASKTMGLFEEARESRKISKSSLKNWVIYASRERTALASTLDD 614
           GSK+IY  D+ RF+ +D+A KT+ LFE A E+ +ISKSSLKNWV+ A RER ALA TL+D
Sbjct: 569 GSKFIYANDIMRFLPDDEALKTLSLFEGASETNRISKSSLKNWVVNAFRERRALALTLND 628

Query: 615 SKTAVKKLHRMVNILISVIILVIWLLILGIATSKFLLFATSQLVLVAFVFGNTCKTVFES 674
           +KTAV +LH+MVNI++ +IILVIWL+ILGI ++KFL+  +SQ+V+VAF+FGN CK VFES
Sbjct: 629 TKTAVNRLHKMVNIVVGIIILVIWLIILGITSTKFLVVMSSQVVVVAFIFGNMCKIVFES 688

Query: 675 IIFLFVMHPFDVGNLCEINGVQMIVKEMNILTTIFLRYDNQKIIIPNSLLATMAIHNFYR 734
           II+LFV+HPFDVG+ CEI+GVQM+V+EMNILTT+FLR+DNQK++ PNSLL T +I N+YR
Sbjct: 689 IIYLFVIHPFDVGDRCEIDGVQMVVEEMNILTTVFLRFDNQKVVYPNSLLWTKSIGNYYR 748

Query: 735 SPDMGDAVEFCLHICTPPEKIAIMRQRIISYIEGKKKHWCPAPRIFLKDVEELNRIRIAI 771
           SPDMGD +EF +HI TP EKI +++QRI SYIEGKK HW PAP I  KD+E LN +RIA+
Sbjct: 749 SPDMGDGIEFSIHITTPAEKIILIKQRITSYIEGKKDHWYPAPMIVFKDMESLNSVRIAV 808

BLAST of Lsi10G008120 vs. Swiss-Prot
Match: MSL5_ARATH (Mechanosensitive ion channel protein 5 OS=Arabidopsis thaliana GN=MSL5 PE=2 SV=1)

HSP 1 Score: 803.9 bits (2075), Expect = 1.5e-231
Identity = 448/832 (53.85%), Postives = 582/832 (69.95%), Query Frame = 1

Query: 2   SGNASLRGMKLVNTSGKIWQESSNDFWNDSDNRLNNGEVTNGNGGGGRSEDFELRQQRK- 61
           SG     G       G IW+ESS DFW D +   N+ +  + +  GG S  F  R +R+ 
Sbjct: 21  SGAVGATGSSSNAEGGNIWKESSYDFW-DGEKGKNDKKGDDEDEDGG-SFHFRQRGERRH 80

Query: 62  ---DVEDPPSKLIGQLLHKQKGSG-EISLDMDMEMLELPQDKTLLPMVADSPMRRR---- 121
              ++ DPPSKLIGQ LHKQ+ SG EISLD+++ M EL  +    P  A +  RR     
Sbjct: 81  SSAELSDPPSKLIGQFLHKQRASGDEISLDVELNMAELQSNTPPRPATASNTPRRGLTTI 140

Query: 122 HRDSLPVDEEHRGKQPQQRDLRAHGSNGEDDGG-----AEVLRCTS-------NSSFGR- 181
              S PV  + +    ++R  R       D+ G     AEVL+C S       N +  R 
Sbjct: 141 SESSSPVKTKVKADAVRRRQNRTSLGGSSDEEGRNRDEAEVLKCGSKKPMLSRNKTKSRL 200

Query: 182 --------------DVSFQRKSGQVRRGLLSKAI-----------DEEDDDPFLEEDVPD 241
                         ++   R+SG  + G L K+            +EE++DPFL+ED+P+
Sbjct: 201 QDPPTPTHPAIDKTEMKSGRRSGIFKSGFLGKSPKAGTPGRNGFEEEEEEDPFLDEDLPE 260

Query: 242 KYKKGNLGVLTLLQWASLILIIAALICTLAIRNWRRKKLWKLELWKWEVMILVLICGRLV 301
           ++K+  L     L+W SL+LI+ +L+C+L I N +RK  WKL+LWKWEV +LVLICGRLV
Sbjct: 261 EFKRDKLSFWVFLEWISLVLIVTSLVCSLTIHNLQRKTWWKLDLWKWEVTVLVLICGRLV 320

Query: 302 SGWGIRIIVFFIERNFLLRKRVLYFVYGIRKAVQNCLWLGLVLIAWNFLFDDKVQREVKS 361
           S W +RIIVF +E+NF  RKRVLYFVYG+RK+VQNCLWLGLVL+AW+FLFD KV+RE +S
Sbjct: 321 SSWIVRIIVFLVEKNFTWRKRVLYFVYGVRKSVQNCLWLGLVLLAWHFLFDKKVERETRS 380

Query: 362 NALEYVTKVLVCLLVSTLVWLVKTLMVKVLASFFHVSTYFDRIQAALFDQYVIGTLLGPP 421
            AL YVT+VLVCLLV+ ++WLVKT++VKVLAS FH+STYFDRIQ +LF QYVI TL GPP
Sbjct: 381 TALRYVTRVLVCLLVALIIWLVKTILVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPP 440

Query: 422 LMEISKNEEEEERVAEEVIKLEN-AGATIPPDLKATAQKGGRV---------------IG 481
           LMEI + EEEE++VAE+V  LE  AGA +PP LKAT +   +V                G
Sbjct: 441 LMEIQRMEEEEQQVAEDVKSLEKLAGAKLPPALKATVKSFMKVGKSPGLNRIGSKRGEDG 500

Query: 482 EGITIDHLHKLSPKNVSAWNMKRLMKIIRHVTLSTLDEQIKDSITHENESTTQIKSGYEA 541
           EGI ID L +++ KNVSAWNMKRLM II    +STLD+ ++D+ T E+E  T I+S YEA
Sbjct: 501 EGIRIDQLKRMNTKNVSAWNMKRLMNIILKGAISTLDQNMQDT-TQEDEDATHIRSEYEA 560

Query: 542 KVAAKKIFLNVARHGSKYIYVEDLKRFMDEDKASKTMGLFEEARESRKISKSSLKNWVIY 601
           K AA+KIF NV   GS+YIY+ED  RF+ E++A + M LFE A ES KISKS LKNWV+ 
Sbjct: 561 KCAARKIFHNVTEPGSRYIYLEDFLRFLCEEEAERAMALFEGASESDKISKSCLKNWVVK 620

Query: 602 ASRERTALASTLDDSKTAVKKLHRMVNILISVIILVIWLLILGIATSKFLLFATSQLVLV 661
           A RER ALA TL+D+KTAV +LHR++N++I +II++IWLLILGIAT++FLL  +SQL+LV
Sbjct: 621 AFRERRALALTLNDTKTAVDRLHRIINVVIGIIIIIIWLLILGIATTRFLLVLSSQLLLV 680

Query: 662 AFVFGNTCKTVFESIIFLFVMHPFDVGNLCEINGVQMIVKEMNILTTIFLRYDNQKIIIP 721
           AFVFGN+CKT+FE+IIFLFVMHPFDVG+ CEI+GVQ++V+EMNILTT+FLRYDNQKII P
Sbjct: 681 AFVFGNSCKTIFEAIIFLFVMHPFDVGDRCEIDGVQLVVEEMNILTTVFLRYDNQKIIYP 740

Query: 722 NSLLATMAIHNFYRSPDMGDAVEFCLHICTPPEKIAIMRQRIISYIEGKKKHWCPAPRIF 771
           NS+L T  I N+YRSPDMGDAVEFC+HI TPPEKI  ++QRI+SY++ KK +W PAP I 
Sbjct: 741 NSVLGTKPIANYYRSPDMGDAVEFCVHIATPPEKITAIKQRILSYVDNKKDYWYPAPMIV 800

BLAST of Lsi10G008120 vs. Swiss-Prot
Match: MSL8_ARATH (Mechanosensitive ion channel protein 8 OS=Arabidopsis thaliana GN=MSL8 PE=2 SV=2)

HSP 1 Score: 796.6 bits (2056), Expect = 2.4e-229
Identity = 407/661 (61.57%), Postives = 529/661 (80.03%), Query Frame = 1

Query: 147 EVLRCTSNSSFGRDVSFQRK----------------------SGQVRRGLLSKAIDEEDD 206
           EV+RCTSN SF R      +                      SGQ++ GLL+  IDEEDD
Sbjct: 222 EVVRCTSNMSFQRKSELISRVKTRSRLQDPPREEETPYSGWRSGQLKSGLLAD-IDEEDD 281

Query: 207 DPFLEEDVPDKYKKGNLGVLTLLQWASLILIIAALICTLAIRNWRRKKLWKLELWKWEVM 266
            P  EEDVPD+YK+G L  +TLLQW SL+ IIAAL C+L+I++W++ ++W L LWKWEV 
Sbjct: 282 -PLAEEDVPDEYKRGKLDAITLLQWLSLVAIIAALACSLSIQSWKKVRVWNLHLWKWEVF 341

Query: 267 ILVLICGRLVSGWGIRIIVFFIERNFLLRKRVLYFVYGIRKAVQNCLWLGLVLIAWNFLF 326
           +LVLICGRLVSGWGIRI+VFFIERNFLLRKRVLYFVYG+R+AVQNCLWLGLVL+AW+FLF
Sbjct: 342 LLVLICGRLVSGWGIRIVVFFIERNFLLRKRVLYFVYGVRRAVQNCLWLGLVLLAWHFLF 401

Query: 327 DDKVQREVKSNALEYVTKVLVCLLVSTLVWLVKTLMVKVLASFFHVSTYFDRIQAALFDQ 386
           D KVQRE +S  L YVTK+LVC L+ST++WL+KTL+VKVLAS FHVSTYFDRIQ ALF+Q
Sbjct: 402 DKKVQRETRSRFLPYVTKILVCFLLSTILWLIKTLVVKVLASSFHVSTYFDRIQEALFNQ 461

Query: 387 YVIGTLLGPPLMEISKNEEEEERVAEEVIKLENAGATIPPDLKATA---QKGGRVIG--- 446
           YVI TL GPP++E+S+ EEEEER  +E+ K++NAGA +PPDL A A    K GRV+    
Sbjct: 462 YVIETLSGPPMIEMSRIEEEEERAQDEIFKMQNAGANLPPDLCAAAFPPGKSGRVMNPKL 521

Query: 447 ----------EGITIDHLHKLSPKNVSAWNMKRLMKIIRHVTLSTLDEQIKDSITHENES 506
                      GI+++HLH+++ KN+SAWNMKRLMKI+R+V+L+TLDEQ+ +S T+E+ES
Sbjct: 522 SPIIPKSTTDNGISMEHLHRMNHKNISAWNMKRLMKIVRNVSLTTLDEQMLES-TYEDES 581

Query: 507 TTQIKSGYEAKVAAKKIFLNVARHGSKYIYVEDLKRFMDEDKASKTMGLFEEARESRKIS 566
           T QI+S  EAK AA+KIF NV + G+KYIY+EDL RF+ ED+A KTMGLFE A E+++IS
Sbjct: 582 TRQIRSEKEAKAAARKIFKNVEQRGAKYIYLEDLMRFLREDEAMKTMGLFEGAPENKRIS 641

Query: 567 KSSLKNWVIYASRERTALASTLDDSKTAVKKLHRMVNILISVIILVIWLLILGIATSKFL 626
           KS+LKNW++ A RER ALA TL+D+KTAV KLH M+NI+ +++I+VIWL++L IA+SK L
Sbjct: 642 KSALKNWLVNAFRERRALALTLNDTKTAVNKLHHMINIVTAIVIVVIWLVLLEIASSKVL 701

Query: 627 LFATSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGNLCEINGVQMIVKEMNILTTIFL 686
           LF +SQ+VL+AF+FGNT KTVFESIIFLF++HP+DVG+ CEI+ VQ++V+EMNILTT+FL
Sbjct: 702 LFVSSQVVLLAFIFGNTVKTVFESIIFLFIVHPYDVGDRCEIDSVQLVVEEMNILTTVFL 761

Query: 687 RYDNQKIIIPNSLLATMAIHNFYRSPDMGDAVEFCLHICTPPEKIAIMRQRIISYIEGKK 746
           RYDN KI+ PNSLL   +I+N+YRSPDMGDA+EFC+HI TP EKI++++QRI +YI+ K 
Sbjct: 762 RYDNLKIMYPNSLLWQKSINNYYRSPDMGDAIEFCVHITTPLEKISVIKQRISNYIDNKP 821

Query: 747 KHWCPAPRIFLKDVEELNRIRIAICLTHRMNHQDMGKRWTRRALLVEELVKIFQEIDLQY 770
           ++W P  +I +KD+E+L+ +R+AI   HR+NHQDM +RWTRRA+LVEE++KI  E+D+Q+
Sbjct: 822 EYWYPQAKIIVKDLEDLHIVRLAIWPCHRINHQDMAERWTRRAVLVEEVIKILLELDIQH 879

BLAST of Lsi10G008120 vs. Swiss-Prot
Match: MSL4_ARATH (Mechanosensitive ion channel protein 4 OS=Arabidopsis thaliana GN=MSL4 PE=3 SV=1)

HSP 1 Score: 736.9 bits (1901), Expect = 2.3e-211
Identity = 424/837 (50.66%), Postives = 558/837 (66.67%), Query Frame = 1

Query: 16  SGKIWQESSNDFW-NDSDNRLNNGEVTNGNGGGGRSEDFELRQQRKDVE-DPPSKLIGQL 75
           S K W+ESS +FW ND  ++   GE  +G      S DF  R   K  E DPPSKLI Q 
Sbjct: 24  SEKFWRESSINFWHNDKSSKPPGGEEDDG------SFDFMRRSSEKSEEPDPPSKLINQF 83

Query: 76  LHKQKGSG-EISLDMDMEMLELPQDKTLLPMVADSPMRRRHRDSLPVDEEHR---GKQPQ 135
           L+KQK SG EISLDM+  M EL Q  T+ P+ + +        + PV   +R   G   +
Sbjct: 84  LNKQKASGDEISLDMEANMPEL-QKNTVPPLSSTAVSGSASPVTAPVTASYRNGTGDAIR 143

Query: 136 QRDLRA-------HGSNGEDD----GGAEVLRCTSNSSFGRDVSFQR------------- 195
           +R  R         G + ED+     G+EV++CTSN S  R  +  +             
Sbjct: 144 RRQNRVTLSPSVKDGDSSEDEENRVDGSEVVKCTSNRSTMRTKTLMKMKTRSRLMDPPTP 203

Query: 196 -----------KSGQVRRGLLSKAID------------EEDDDPFLEEDVPDKYKKGNLG 255
                      +SG +  G   +               EE++DPF EED+P+  +K  + 
Sbjct: 204 TYPDMVSGRTPRSGNLNPGFSGRNTKPGTPNQGGSKDLEEEEDPFSEEDLPEGLRKEKIC 263

Query: 256 VLTLLQWASLILIIAALICTLAIRNWRRKKLWKLELWKWEVMILVLICGRLVSGWGIRII 315
           V  +++W  LILIIA+LIC+L I   R K LW L LWKWEVM+LVLICGRLVS W +++ 
Sbjct: 264 VWVIIEWIFLILIIASLICSLVIPYLRGKTLWDLALWKWEVMVLVLICGRLVSSWIVKLF 323

Query: 316 VFFIERNFLLRKRVLYFVYGIRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNALEYVTK 375
           V+F+E NFL RK+VLYFVYGIRK VQNCLWLGLVLIAW+FLFD KV+RE++S  L+YVTK
Sbjct: 324 VYFVESNFLWRKKVLYFVYGIRKPVQNCLWLGLVLIAWHFLFDKKVEREMRSTVLKYVTK 383

Query: 376 VLVCLLVSTLVWLVKTLMVKVLASFFHVSTYFDRIQAALFDQYVIGTLLGPPLMEISKNE 435
           VL+CLLV+ ++WL+KTL+VKVLAS FH+STYFDRIQ +LF QYVI TL GPP +EI    
Sbjct: 384 VLICLLVAVIIWLIKTLLVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPRIEI---H 443

Query: 436 EEEERVAEEVIKLENAGATI----------PPDL---KATAQKGGRVIGEGITIDHLH-- 495
            EEE+VA +V   E  G  +          PP +       QK    +G+   +      
Sbjct: 444 IEEEKVANDVKTFEIVGRKLSPLGPKAVSSPPQVTVGSGRLQKSPSRVGKSPVLSRSGSK 503

Query: 496 --------------KLSPKNVSAWNMKRLMKIIRHVTLSTLDEQIKDSITHENESTTQIK 555
                         +++ KNVSAW MK+LM +I+  TLSTLDEQI+D+ T E++  TQI+
Sbjct: 504 KEGGEEGIRIDHLQRMNTKNVSAWKMKKLMNVIKKGTLSTLDEQIQDTTTQEDDKATQIR 563

Query: 556 SGYEAKVAAKKIFLNVARHGSKYIYVEDLKRFMDEDKASKTMGLFEEARESRKISKSSLK 615
           S +EAK+AA+KIF NVA  GS+YIY+ED  RF+ ED++ + M LFE A E  KISKS LK
Sbjct: 564 SEFEAKLAARKIFQNVAEPGSRYIYMEDFMRFLSEDESERAMDLFEGASECHKISKSCLK 623

Query: 616 NWVIYASRERTALASTLDDSKTAVKKLHRMVNILISVIILVIWLLILGIATSKFLLFATS 675
           NWV+ A RER ALA TL+D+KTAV +LHR+V++L+S++IL+IWLLILGIAT+KFLL  +S
Sbjct: 624 NWVVNAFRERRALALTLNDTKTAVNRLHRIVDVLVSIVILIIWLLILGIATTKFLLVISS 683

Query: 676 QLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGNLCEINGVQMIVKEMNILTTIFLRYDNQ 735
           QL+LV FVFGN+CKT+FE++IF+FVMHPFDVG+ CEI+GVQMIV+EMNILTT+FLR+DNQ
Sbjct: 684 QLLLVVFVFGNSCKTIFEAVIFVFVMHPFDVGDRCEIDGVQMIVEEMNILTTVFLRFDNQ 743

Query: 736 KIIIPNSLLATMAIHNFYRSPDMGDAVEFCLHICTPPEKIAIMRQRIISYIEGKKKHWCP 771
           KI+ PNSLL T  I N+YRSPDM DA+EF +HI TPPEK   +RQRI+SY++ KK HW P
Sbjct: 744 KIVYPNSLLGTKPIANYYRSPDMQDAIEFFVHIATPPEKTTALRQRILSYVDNKKDHWHP 803

BLAST of Lsi10G008120 vs. Swiss-Prot
Match: MSL7_ARATH (Mechanosensitive ion channel protein 7 OS=Arabidopsis thaliana GN=MSL7 PE=3 SV=1)

HSP 1 Score: 719.9 bits (1857), Expect = 2.9e-206
Identity = 374/621 (60.23%), Postives = 487/621 (78.42%), Query Frame = 1

Query: 167 SGQVRRGLLSKAID--EEDDDPFLEEDVPDKYKKGNLGVLTLLQWASLILIIAALICTLA 226
           S Q+R GLL +  D  EE+DD   EEDVP +Y+K  +  +TLLQW SLI ++ AL+ +L 
Sbjct: 193 SDQLRSGLLGRHSDDIEEEDDSSAEEDVPVEYRKLKMDAITLLQWMSLIALVVALVLSLG 252

Query: 227 IRNWRRKKLWKLELWKWEVMILVLICGRLVSGWGIRIIVFFIERNFLLRKRVLYFVYGIR 286
           +  WR   LW L LWKWEV++LVLICGRLVSG GIRIIVFFIERNFLLRKRVLYFVYG++
Sbjct: 253 LHTWRNATLWSLHLWKWEVVLLVLICGRLVSGCGIRIIVFFIERNFLLRKRVLYFVYGVK 312

Query: 287 KAVQNCLWLGLVLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLVSTLVWLVKTLMVKVL 346
            AVQNCLWLGLVL+AW+FLFD KV++E +S+ L  ++K+LVC L+ST++WL+KTL+VKVL
Sbjct: 313 TAVQNCLWLGLVLLAWHFLFDKKVEKETQSDVLLLMSKILVCFLLSTVLWLIKTLVVKVL 372

Query: 347 ASFFHVSTYFDRIQAALFDQYVIGTLLGPPLMEISKNEEEEERVAEEVIKLENAGATIPP 406
           AS FHVSTYFDRIQ ALF  Y+I TL GPP++E+S+ EEEE+R  +E+ K++  GA + P
Sbjct: 373 ASSFHVSTYFDRIQEALFHHYLIETLSGPPMLELSRIEEEEDRTQDEIYKMQKGGADLSP 432

Query: 407 DLKATA---QKGGRVIG-------------EGITIDHLHKLSPKNVSAWNMKRLMKIIRH 466
           +L + A   +K G  +               GIT+D LHK++ KNVSAWNMKRLMKI+R+
Sbjct: 433 ELCSAAFPQEKSGSTMNMKFSPIIPKTGSDNGITMDDLHKMNQKNVSAWNMKRLMKIVRN 492

Query: 467 VTLSTLDEQIKDSITHENESTTQIKSGYEAKVAAKKIFLNVARHGSKYIYVEDLKRFMDE 526
           V+LSTLDEQ   + T E+EST QI+S  EAK AA+KIF NVA+ G+K+IY+EDL RF+  
Sbjct: 493 VSLSTLDEQALQN-TCEDESTRQIRSEKEAKAAARKIFKNVAQPGTKHIYLEDLMRFLRV 552

Query: 527 DKASKTMGLFEEARESRKISKSSLKNWVIYASRERTALASTLDDSKTAVKKLHRMVNILI 586
           D+A KTM LFE A  ++KI+KS+LKNW++ A RER ALA TL+D+KTAV KLH M++ L 
Sbjct: 553 DEAMKTMCLFEGALVTKKITKSALKNWLVNAFRERRALALTLNDTKTAVNKLHHMISFLT 612

Query: 587 SVIILVIWLLILGIATSKFLLFATSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGNLC 646
           +++I+VIWL++L IATSK+LLF TSQ+VL+AF+FGN+ KTVFESIIFLF++HP+DVG+  
Sbjct: 613 AIVIIVIWLILLEIATSKYLLFLTSQVVLLAFMFGNSLKTVFESIIFLFIIHPYDVGDRL 672

Query: 647 EINGVQMIVKEMNILTTIFLRYDNQKIIIPNSLLATMAIHNFYRSPDMGDAVEFCLHICT 706
            I+ V+M+V+EMNILTT+FLR DN KI+ PN LL   AIHN+ RSPDMGD V  C+HI T
Sbjct: 673 LIDTVEMVVEEMNILTTVFLRADNLKIVYPNILLWQKAIHNYNRSPDMGDEVTCCVHITT 732

Query: 707 PPEKIAIMRQRIISYIEGKKKHWCPAPRIFLKDVEELNRIRIAICLTHRMNHQDMGKRWT 766
           PPEKIA ++QRI SYI+ K ++W P   + +KDVE+LN +RIAI L H++NHQ+MG+R+T
Sbjct: 733 PPEKIAAIKQRISSYIDSKPEYWYPKADVIVKDVEDLNIVRIAIWLCHKINHQNMGERFT 792

Query: 767 RRALLVEELVKIFQEIDLQYR 770
           RRALL+EE++KI  E+D+QYR
Sbjct: 793 RRALLIEEVIKILLELDIQYR 812

BLAST of Lsi10G008120 vs. TrEMBL
Match: A0A0A0KE62_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G124170 PE=4 SV=1)

HSP 1 Score: 1122.5 bits (2902), Expect = 0.0e+00
Identity = 623/841 (74.08%), Postives = 676/841 (80.38%), Query Frame = 1

Query: 5   ASLRGMKLVNTSGKIWQESSNDFWNDSDNRLNNGE----VTNGNGGGGRSEDFE------ 64
           ++LR   LVN SG IW+ESS DFWNDSDNR N GE     T+        +D E      
Sbjct: 64  STLRATDLVNGSGTIWRESSYDFWNDSDNRRNYGEGGAKTTDDFEFRQHRKDVEDPPSKL 123

Query: 65  ----LRQQRKDVE---DPPSKLIGQLLHKQKGSGEISLDMDMEMLELPQDKTLLPMVADS 124
               L +Q+   E   D   +++     K   S      M     EL      +  ++++
Sbjct: 124 IGQFLHKQKASGEMSLDMDMEMLELPQDKTPLSTVAESPMRRSSRELKVSFESISEISEN 183

Query: 125 P-MRRRHRDSLPVDEEHRGKQPQQRDLRAHGSNGEDDGGAEVLRCTSNSSFGRDVSFQRK 184
             MRRRHRDS P+DEEHRG+QP+Q D RAHGSNGEDDG AEVLRC+SNS F RDVSFQRK
Sbjct: 184 DSMRRRHRDS-PLDEEHRGQQPRQCDRRAHGSNGEDDGAAEVLRCSSNSFFQRDVSFQRK 243

Query: 185 S----------------------------GQVRRGLLSKAIDEEDDDPFLEEDVPDKYKK 244
           S                            GQVR GL+SKA+DEEDDDPFLEED+PD+YKK
Sbjct: 244 SSLLRAKTKSRLLDPPEHQDRRSGRVPKSGQVRSGLISKALDEEDDDPFLEEDLPDEYKK 303

Query: 245 GNLGVLTLLQWASLILIIAALICTLAIRNWRRKKLWKLELWKWEVMILVLICGRLVSGWG 304
            NLGVLTLLQWASLILIIAAL+CTL IR WRRKKLWKLE+WKWEVMILVLICGRLVSGWG
Sbjct: 304 ANLGVLTLLQWASLILIIAALVCTLTIRYWRRKKLWKLEVWKWEVMILVLICGRLVSGWG 363

Query: 305 IRIIVFFIERNFLLRKRVLYFVYGIRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNALE 364
           IR+IVFFIERNFLLRKRVLYFVYG+RKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNALE
Sbjct: 364 IRVIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNALE 423

Query: 365 YVTKVLVCLLVSTLVWLVKTLMVKVLASFFHVSTYFDRIQAALFDQYVIGTLLGPPLMEI 424
           YVTKVLVCLLVSTLVWLVKTLMVKVLAS FHVSTYFDRIQ ALF+QYVI TL GPPL+EI
Sbjct: 424 YVTKVLVCLLVSTLVWLVKTLMVKVLASSFHVSTYFDRIQDALFNQYVIETLSGPPLIEI 483

Query: 425 SKNEEEEERVAEEVIKLENAGATIPPDLKATA----QKGGRVIG---------------- 484
            KNEEEEER+AEEVIKL+NAGATIPPDLKATA    QKGGRVIG                
Sbjct: 484 QKNEEEEERLAEEVIKLQNAGATIPPDLKATAFSTAQKGGRVIGSGGLQKSPRGRSGKLS 543

Query: 485 ---------EGITIDHLHKLSPKNVSAWNMKRLMKIIRHVTLSTLDEQIKDSITHENEST 544
                    EGITIDHLHKLSPKNVSAWNMKRLM I+RH TLSTLDEQIKD+  HE+EST
Sbjct: 544 RTLSKKGGDEGITIDHLHKLSPKNVSAWNMKRLMNIVRHGTLSTLDEQIKDT-AHEDEST 603

Query: 545 TQIKSGYEAKVAAKKIFLNVARHGSKYIYVEDLKRFMDEDKASKTMGLFEEARESRKISK 604
           T+IKS YEAKVAAKKIFLNVAR+GSKYIY+EDL RFM++D+ASKTMGLFE A ESRKISK
Sbjct: 604 TEIKSEYEAKVAAKKIFLNVARNGSKYIYLEDLMRFMEKDEASKTMGLFEGACESRKISK 663

Query: 605 SSLKNWVIYASRERTALASTLDDSKTAVKKLHRMVNILISVIILVIWLLILGIATSKFLL 664
           SSLKNWV+ A RER ALA TL+D+KTAV KLHRMVNIL+SVIILVIWLLILGIATSKFLL
Sbjct: 664 SSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLILGIATSKFLL 723

Query: 665 FATSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGNLCEINGVQMIVKEMNILTTIFLR 724
           F TSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVG+ CEI+GVQMIV+EMNILTTIFLR
Sbjct: 724 FVTSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTIFLR 783

Query: 725 YDNQKIIIPNSLLATMAIHNFYRSPDMGDAVEFCLHICTPPEKIAIMRQRIISYIEGKKK 771
           YDNQKII PNS+LAT AIHNFYRSPDMGD +EFCLHI TPPEKIAIMRQRIISYIEGKK+
Sbjct: 784 YDNQKIIFPNSVLATKAIHNFYRSPDMGDGIEFCLHISTPPEKIAIMRQRIISYIEGKKE 843

BLAST of Lsi10G008120 vs. TrEMBL
Match: A0A061FGA7_THECC (Mechanosensitive channel of small conductance-like 6, putative OS=Theobroma cacao GN=TCM_032110 PE=4 SV=1)

HSP 1 Score: 941.0 bits (2431), Expect = 8.9e-271
Identity = 520/836 (62.20%), Postives = 621/836 (74.28%), Query Frame = 1

Query: 17  GKIWQESSNDFWNDSD---------NRLNNGEVTNGNGGGGRSEDFE-LRQQRKDVEDPP 76
           GKIW+ESS DFW DS+         N   NG  ++ N     SE F+ +R ++  +EDPP
Sbjct: 37  GKIWRESSYDFWKDSEKISSNWKKENANMNGTGSSANNSSRESEGFDFMRSKQAAIEDPP 96

Query: 77  SKLIGQLLHKQKGSGEISLDMDMEMLELPQDK----TLLPMVADSPMRRRHRDSLPVDEE 136
           SKLIGQ LHKQK SGEISLDMD+EM EL Q+     TLLP VA+SP            E 
Sbjct: 97  SKLIGQFLHKQKASGEISLDMDLEMDELQQEPPHHGTLLPTVAESPSPSAAAVPRVSFEN 156

Query: 137 HRGKQPQQRDLRAHGSNGEDDG--GAEVLRCTSNSSFGRDV--SFQRKS----------- 196
           +  ++ Q +   + G     D   G  V++C+SNSSF R    SFQRKS           
Sbjct: 157 NAVRRRQSKGSASPGKEESRDSKEGDGVVKCSSNSSFKRSEGGSFQRKSSLLATKTKSRL 216

Query: 197 ---------------------GQVRR-GLLSKAIDEEDDDPFLEEDVPDKYKKGNLGVLT 256
                                GQ+ R G L K+++EE+DDP LEED+PD+YKK  L VL 
Sbjct: 217 MDPPTPEKGEPRSAKATVGKSGQIMRSGFLGKSMEEEEDDPLLEEDLPDEYKKDKLSVLV 276

Query: 257 LLQWASLILIIAALICTLAIRNWRRKKLWKLELWKWEVMILVLICGRLVSGWGIRIIVFF 316
           LL+W SLILI+AA +C+L I   R K+LW L LWKWEV++LVLICGRLVSGW IRIIVFF
Sbjct: 277 LLEWLSLILIVAAFVCSLTIPYLRAKRLWSLMLWKWEVLVLVLICGRLVSGWIIRIIVFF 336

Query: 317 IERNFLLRKRVLYFVYGIRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNALEYVTKVLV 376
           IERNFLLRKRVLYFVYG+RKAVQNCLWLGLVLIAW++LFD KVQRE KS  L YVTKVLV
Sbjct: 337 IERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWHYLFDKKVQRETKSEFLRYVTKVLV 396

Query: 377 CLLVSTLVWLVKTLMVKVLASFFHVSTYFDRIQAALFDQYVIGTLLGPPLMEISKNEEEE 436
           CL+V  ++WLVKTL+VKVLAS FHVSTYFDRIQ +LF+QYV+ TL GPPL+EI + EEEE
Sbjct: 397 CLVVGVMLWLVKTLLVKVLASSFHVSTYFDRIQDSLFNQYVVETLSGPPLIEIQRAEEEE 456

Query: 437 ERVAEEVIKLENAGATIPPDLKAT---AQKGGRVIG------------------------ 496
           ER+A EV  L+ AGATIPP LK +   +   G+VIG                        
Sbjct: 457 ERIANEVKNLQKAGATIPPGLKTSTLSSPHSGKVIGSGRIQKSPRGKSPMLSRMLSSEKG 516

Query: 497 ----EGITIDHLHKLSPKNVSAWNMKRLMKIIRHVTLSTLDEQIKDSITHENESTTQIKS 556
               +GITIDHLHKL+ KNVSAWNMKRLM IIRH  LSTLDEQI+DS THE+ES TQI+S
Sbjct: 517 EKDKKGITIDHLHKLNHKNVSAWNMKRLMNIIRHGALSTLDEQIQDS-THEDESATQIRS 576

Query: 557 GYEAKVAAKKIFLNVARHGSKYIYVEDLKRFMDEDKASKTMGLFEEARESRKISKSSLKN 616
            YEAKVAA+KIF NVA+ GSKYIY+ED++RF+ ED+A KTM LFE A ESR+ISK +LKN
Sbjct: 577 EYEAKVAARKIFQNVAKPGSKYIYLEDIERFLQEDEAFKTMSLFEGASESRRISKKALKN 636

Query: 617 WVIYASRERTALASTLDDSKTAVKKLHRMVNILISVIILVIWLLILGIATSKFLLFATSQ 676
           WV+ A RER ALA TL+D+KTAV +LHRMVN+L+ +II+VIWLLILGIA+SK L+F +SQ
Sbjct: 637 WVVNAFRERRALALTLNDTKTAVNRLHRMVNVLVGIIIVVIWLLILGIASSKVLVFISSQ 696

Query: 677 LVLVAFVFGNTCKTVFESIIFLFVMHPFDVGNLCEINGVQMIVKEMNILTTIFLRYDNQK 736
           L+LVAF+FGNTCKTVFE+IIFLFVMHPFDVG+ CEI+GVQM+V+EMNILTT+FLRYDNQK
Sbjct: 697 LLLVAFIFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMVVEEMNILTTVFLRYDNQK 756

Query: 737 IIIPNSLLATMAIHNFYRSPDMGDAVEFCLHICTPPEKIAIMRQRIISYIEGKKKHWCPA 771
           IIIPNS+LAT AI+N+YRSPDMGDAVEFC+H+ TP EKI +M+QRI+SYIE K  HW PA
Sbjct: 757 IIIPNSVLATKAINNYYRSPDMGDAVEFCIHVKTPAEKIGLMKQRILSYIEHKSDHWYPA 816

BLAST of Lsi10G008120 vs. TrEMBL
Match: A0A059D715_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_B03162 PE=4 SV=1)

HSP 1 Score: 930.2 bits (2403), Expect = 1.6e-267
Identity = 513/822 (62.41%), Postives = 610/822 (74.21%), Query Frame = 1

Query: 18  KIWQESSNDFWNDSDNRLNNGEVTNGNGGGGRS---------EDFELR---QQRKDVEDP 77
           KIW+ SS DFW D        E   G+GGGG S         ++F+ R   ++    EDP
Sbjct: 7   KIWRGSSYDFWKD--------EEVGGSGGGGASGAGPRVSDGDEFDFRKGGEKGSGSEDP 66

Query: 78  PSKLIGQLLHKQKGSGEISLDMDMEMLELPQDK--------TLLPMVADSPMRRRHRDSL 137
           PSKLIGQ LHKQ+ SG+ +LD+D+EM EL QD+          LP + +SPM+ R++ S 
Sbjct: 67  PSKLIGQFLHKQRASGDFTLDIDLEMQELKQDEDNDDGDTFAPLPPLEESPMKSRNKVSF 126

Query: 138 --PVDEEHRGKQPQQRDLRAHGSNGEDDGGAEVLRCTSNSSFGRDVSFQRKS----GQVR 197
             P   E R  +      +     G +DG   VLRCTSNSSF  + SF+RKS    G V+
Sbjct: 127 GEPGSSEGRTARVSVESQQRQQDEGSEDG--PVLRCTSNSSFQSNSSFKRKSRLLTGAVK 186

Query: 198 -------------------------RGLLSKAIDEEDDDPFLEEDVPDKYKKGNLGVLTL 257
                                    +G   +  DE+++DPF  ED+PD+YKKG L ++ L
Sbjct: 187 NKSRLLDPPDHADRRSGRVSKLGTPKGTPKRGGDEDEEDPFAGEDLPDQYKKGRLELVVL 246

Query: 258 LQWASLILIIAALICTLAIRNWRRKKLWKLELWKWEVMILVLICGRLVSGWGIRIIVFFI 317
           L+W SL+LI+AAL+C+L I   R+KKLW L LWKWEVM+LVLICGRLVS WGIRI VFFI
Sbjct: 247 LEWVSLVLIVAALVCSLTIPYLRKKKLWDLMLWKWEVMVLVLICGRLVSSWGIRIAVFFI 306

Query: 318 ERNFLLRKRVLYFVYGIRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNALEYVTKVLVC 377
           ERNF+LRKRVLYFVYG+RKAVQNCLWLGLVL+AW+FLFD KVQRE  S AL+ VTK+LVC
Sbjct: 307 ERNFVLRKRVLYFVYGLRKAVQNCLWLGLVLLAWHFLFDKKVQRETNSEALQVVTKILVC 366

Query: 378 LLVSTLVWLVKTLMVKVLASFFHVSTYFDRIQAALFDQYVIGTLLGPPLMEISKNEEEEE 437
           LLV TL+WLVKTL+VKVLAS FHVSTYFDRIQ +LF+QYVI TL GPPL+E+ K EEEEE
Sbjct: 367 LLVGTLLWLVKTLIVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLIEMRKAEEEEE 426

Query: 438 RVAEEVIKLENAGATIPPDLKAT---AQKGGRVIG---------------EGITIDHLHK 497
           ++A EV KL+ AGA IPPDL A    A+  GRVIG               EGITIDHLHK
Sbjct: 427 KIAVEVEKLKKAGAVIPPDLNAAVLPAKGSGRVIGSGIVQKSPRGKDKGEEGITIDHLHK 486

Query: 498 LSPKNVSAWNMKRLMKIIRHVTLSTLDEQIKDSITHENESTTQIKSGYEAKVAAKKIFLN 557
           LSPKNVSAWNMKRLM ++R+  L+TLDEQ+    +HE+ES+ QI S  EA+ AAKKIF N
Sbjct: 487 LSPKNVSAWNMKRLMNLVRYGNLTTLDEQLS---SHEDESSNQINSEGEARAAAKKIFQN 546

Query: 558 VARHGSKYIYVEDLKRFMDEDKASKTMGLFEEARESRKISKSSLKNWVIYASRERTALAS 617
           VAR GSK+IY+EDL RFM ED+A KTM LFE A ES+KISKSSLKNWV+ A RER ALA 
Sbjct: 547 VARQGSKHIYLEDLMRFMPEDEAVKTMSLFEGASESQKISKSSLKNWVVNAFRERRALAL 606

Query: 618 TLDDSKTAVKKLHRMVNILISVIILVIWLLILGIATSKFLLFATSQLVLVAFVFGNTCKT 677
           TLDD+KTAV KLHRMVNIL+ ++I+VIWLLILGIAT KFLL  +SQL+LV F+FGN+CK 
Sbjct: 607 TLDDTKTAVNKLHRMVNILVGILIVVIWLLILGIATKKFLLLISSQLLLVVFIFGNSCKM 666

Query: 678 VFESIIFLFVMHPFDVGNLCEINGVQMIVKEMNILTTIFLRYDNQKIIIPNSLLATMAIH 737
            FE+IIFLFVMHPFDVG+ CEI+GVQM+V+EMNILTTIFLRYDNQKIIIPNSLLAT AI 
Sbjct: 667 TFEAIIFLFVMHPFDVGDRCEIDGVQMVVEEMNILTTIFLRYDNQKIIIPNSLLATKAIS 726

Query: 738 NFYRSPDMGDAVEFCLHICTPPEKIAIMRQRIISYIEGKKKHWCPAPRIFLKDVEELNRI 771
           N+YRSPDMGDAVEFC+HI TP EKIA+M+QRI SYIE K  HW PAP I  KDVEELN++
Sbjct: 727 NYYRSPDMGDAVEFCVHISTPTEKIAVMKQRITSYIENKNDHWYPAPMIIFKDVEELNKL 786

BLAST of Lsi10G008120 vs. TrEMBL
Match: V4T5R5_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10018823mg PE=4 SV=1)

HSP 1 Score: 907.5 bits (2344), Expect = 1.1e-260
Identity = 495/860 (57.56%), Postives = 607/860 (70.58%), Query Frame = 1

Query: 1   MSGNASLRGMKLVNTSGKIWQESSNDFWNDSDNRLNNGEVTNGNGGGGRSEDFELRQQRK 60
           + GN       + + + KIW+ SS DFW D     NNG              F+  Q   
Sbjct: 15  VDGNKPNNSSSVSSNNSKIWRGSSYDFWEDRGKMDNNG--------------FDFMQHGP 74

Query: 61  DVEDPPSKLIGQLLHKQKGSGEISLDMDMEMLELPQDKTL-------------------- 120
            +EDPPS LIGQ LHKQK SGEISLDMD+EM EL    +                     
Sbjct: 75  SMEDPPSVLIGQFLHKQKASGEISLDMDLEMDELQHQASSKNNNCGSSSGININSNNKST 134

Query: 121 --LPMVADSP----------MRRRHRDSLPVDEEHRGKQPQQRDLRAHGSNGEDDGGAEV 180
             LP V++SP          ++RRH +S      +    PQ+            D   EV
Sbjct: 135 QGLPTVSESPTAVNRVSFESLKRRHSNST---NNNYKDSPQK------------DSEGEV 194

Query: 181 LRCTSNSSFGRDVSFQRKS------------------------------GQVRRGLLSKA 240
           ++CTSN SF R+VSF +KS                              GQ++ G + K 
Sbjct: 195 VKCTSNKSFDRNVSFNKKSALLMTKTKSRLMDLPPERMEPKSGRVVGRSGQLKSGFIGKN 254

Query: 241 IDEEDDDPFLEEDVPDKYKKGNLGVLTLLQWASLILIIAALICTLAIRNWRRKKLWKLEL 300
           +DEE++DP LEED+P++YKK  + +  LL+W SLILII AL+C+L I  +++KKLWKL L
Sbjct: 255 VDEEEEDPLLEEDLPEEYKKEKISIWVLLEWFSLILIIGALVCSLTIDYFKKKKLWKLGL 314

Query: 301 WKWEVMILVLICGRLVSGWGIRIIVFFIERNFLLRKRVLYFVYGIRKAVQNCLWLGLVLI 360
           WKWE++ILVLICGRLVS W +RIIVF IERNFLLRKRVLYFVYG+RKAVQNCLWLGLVLI
Sbjct: 315 WKWELLILVLICGRLVSSWIVRIIVFCIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLI 374

Query: 361 AWNFLFDDKVQREVKSNALEYVTKVLVCLLVSTLVWLVKTLMVKVLASFFHVSTYFDRIQ 420
           AW+ LFD +V+RE  S+ L+Y TK+L+CL V  ++WLVKTL+VKVLAS FHVSTYFDRIQ
Sbjct: 375 AWHCLFDQRVERETNSDVLKYATKILICLWVGVMLWLVKTLLVKVLASSFHVSTYFDRIQ 434

Query: 421 AALFDQYVIGTLLGPPLMEISKNEEEEERVAEEVIKLENAGATIPPDLKAT---AQKGGR 480
            ALF+QY+I TL GPPL+EI K EEE+ER+  EV KL+NAG TIPP LK++   + +  +
Sbjct: 435 EALFNQYLIETLSGPPLIEIQKAEEEQERIVSEVQKLQNAGVTIPPGLKSSVLSSPQSAK 494

Query: 481 VIG-------------------------EGITIDHLHKLSPKNVSAWNMKRLMKIIRHVT 540
           VIG                         +GITIDHLHKL+PKNVSAWNMKRLM IIRH +
Sbjct: 495 VIGSGRLQRTPREGKSPKLSRTFSNKDDDGITIDHLHKLNPKNVSAWNMKRLMNIIRHGS 554

Query: 541 LSTLDEQIKDSITHENESTTQIKSGYEAKVAAKKIFLNVARHGSKYIYVEDLKRFMDEDK 600
           L+TLDEQI+DS THE+ES   IK+ YEAK AA+KIF NVA+ GSK+IY+EDL RF++ED+
Sbjct: 555 LTTLDEQIQDS-THEDESAPHIKTEYEAKAAARKIFQNVAKPGSKFIYLEDLMRFLNEDE 614

Query: 601 ASKTMGLFEEARESRKISKSSLKNWVIYASRERTALASTLDDSKTAVKKLHRMVNILISV 660
           ASKTM LFEEA E +KISKS+LKNWV+   RER ALA TL+D+KTAV KLHR VN+LI +
Sbjct: 615 ASKTMSLFEEASERKKISKSALKNWVVNVFRERRALAFTLNDTKTAVNKLHRFVNVLIGI 674

Query: 661 IILVIWLLILGIATSKFLLFATSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGNLCEI 720
           I ++IWLLILGIAT+KFLLF +SQ+V+VAF+FGNTCKTVFE+IIFLFVMHPFDVG+ CEI
Sbjct: 675 ITVIIWLLILGIATTKFLLFISSQVVVVAFIFGNTCKTVFEAIIFLFVMHPFDVGDRCEI 734

Query: 721 NGVQMIVKEMNILTTIFLRYDNQKIIIPNSLLATMAIHNFYRSPDMGDAVEFCLHICTPP 771
           +GVQM+V+EMNIL+T+FLRYDNQKII PNS+LAT AI N+YRSPDMGDAVEFC+HI TP 
Sbjct: 735 DGVQMVVEEMNILSTVFLRYDNQKIIFPNSVLATKAIGNYYRSPDMGDAVEFCIHISTPA 794

BLAST of Lsi10G008120 vs. TrEMBL
Match: A0A0A0K619_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G017150 PE=4 SV=1)

HSP 1 Score: 899.8 bits (2324), Expect = 2.3e-258
Identity = 483/843 (57.30%), Postives = 614/843 (72.84%), Query Frame = 1

Query: 14  NTSGKIWQESSNDFWNDSDNRLNNGEVTNGNGGGGRSEDFELRQQRKDVEDPPSKLIGQL 73
           N  G +W+ES  DFWN+ +  +        N G  R+E FE  Q    +EDPP+KLIG  
Sbjct: 88  NNGGNVWRESRYDFWNNDEIGIGESARMGDNSGVDRNEGFEFVQTGYGMEDPPTKLIGDF 147

Query: 74  LHKQKGSGEISLDMDMEMLELPQDKTLLPMVADSPM------------------------ 133
           L KQK  GE +LDMD+EM EL  ++ ++P +A+SP+                        
Sbjct: 148 LCKQKIEGETTLDMDLEMEELKPNR-IVPPLAESPLSQTSKDLKVSFQHDSTEISSNDQS 207

Query: 134 -RRRHRDSLPVDEEHRG-----KQPQQRDLRAHGSNG-EDDGGAEVLRCTSNSSFGRDVS 193
            RRR+RDS  + EE +G     +QP    L +   +G +++  AE +RC SN SF  ++S
Sbjct: 208 IRRRNRDSNDLKEESKGGQSPRQQPHHERLGSPTISGVQNESLAEAMRCASNLSFHSELS 267

Query: 194 FQR----------------------------KSGQVRRGLLSKAIDEEDDDPFLEEDVPD 253
           FQR                            KSGQ+R G L K I+++DDDPFLE+D+PD
Sbjct: 268 FQRKSNLLRAKTKSRLIDPPAEPDRLSGLIPKSGQLRSGFLGK-IEDDDDDPFLEDDLPD 327

Query: 254 KYKKGNLGVLTLLQWASLILIIAALICTLAIRNWRRKKLWKLELWKWEVMILVLICGRLV 313
           ++K+GN   LT+LQW SLI+I AALICTL++   R K LW+L++WKWEVMI +LICGRLV
Sbjct: 328 EFKRGNFSALTVLQWVSLIIITAALICTLSVPYLREKSLWELDIWKWEVMIFILICGRLV 387

Query: 314 SGWGIRIIVFFIERNFLLRKRVLYFVYGIRKAVQNCLWLGLVLIAWNFLFDDKVQREVKS 373
           SGWGIRI VFFIERNFLLRKRVLYFVYG+RK VQNCLWLGLVLIAW+ LF+ +V+++  +
Sbjct: 388 SGWGIRIGVFFIERNFLLRKRVLYFVYGVRKPVQNCLWLGLVLIAWHLLFNKRVEKQTNT 447

Query: 374 NALEYVTKVLVCLLVSTLVWLVKTLMVKVLASFFHVSTYFDRIQAALFDQYVIGTLLGPP 433
           + L YV++VLVCLL+STL+WLVKTLMVKVLAS FHVSTYFDRIQ +LF+QYVI TL GPP
Sbjct: 448 SILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPP 507

Query: 434 LMEISKNEEEEERVAEEVIKLENAGATIPPDLKA---TAQKGGRVIG------------- 493
           L+EI KNEEEEER+A+EV KL+NAG  IPPDLKA    + K GR IG             
Sbjct: 508 LVEIRKNEEEEERIADEVQKLQNAGIIIPPDLKAATFASIKSGREIGSGRTHKSFCAKSC 567

Query: 494 -----------EGITIDHLHKLSPKNVSAWNMKRLMKIIRHVTLSTLDEQIKDSITHENE 553
                      +GITIDHLHKLS KNVSAWNMKRL+ I+R+ ++STLDEQI+     ++E
Sbjct: 568 KLSRALTKNRNDGITIDHLHKLSTKNVSAWNMKRLLNIVRYGSISTLDEQIRGPCL-DDE 627

Query: 554 STTQIKSGYEAKVAAKKIFLNVARHGSKYIYVEDLKRFMDEDKASKTMGLFEEARESRKI 613
           STT+IKS  EAK AAKKIF NVA  G KYIY++DL RFM ED+  KTM LFE A E+++I
Sbjct: 628 STTEIKSEREAKAAAKKIFQNVAHRGYKYIYLDDLVRFMREDEVLKTMSLFEGAAENQRI 687

Query: 614 SKSSLKNWVIYASRERTALASTLDDSKTAVKKLHRMVNILISVIILVIWLLILGIATSKF 673
           SKS+LKNWV+ A RER ALA TL+D+KTAV KLH MVN++  ++IL++WL++LGIA+SKF
Sbjct: 688 SKSALKNWVVNAFRERRALALTLNDTKTAVDKLHHMVNVIFGILILILWLIVLGIASSKF 747

Query: 674 LLFATSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGNLCEINGVQMIVKEMNILTTIF 733
            +F +SQ+V+VAF+FGNTCKT+FE+IIFLFVMHPFDVG+ CEI+G+QM+V+EMNILTT+F
Sbjct: 748 FIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDRCEIDGMQMVVEEMNILTTVF 807

Query: 734 LRYDNQKIIIPNSLLATMAIHNFYRSPDMGDAVEFCLHICTPPEKIAIMRQRIISYIEGK 771
           LRYDN K+IIPNS+LAT  IHNFYRSPDMG+++EF +HI TP EKI  M+ RIISYIEG 
Sbjct: 808 LRYDNLKVIIPNSVLATKLIHNFYRSPDMGESIEFLVHIATPAEKITAMKHRIISYIEGN 867

BLAST of Lsi10G008120 vs. TAIR10
Match: AT1G78610.1 (AT1G78610.1 mechanosensitive channel of small conductance-like 6)

HSP 1 Score: 808.5 bits (2087), Expect = 3.5e-234
Identity = 451/818 (55.13%), Postives = 580/818 (70.90%), Query Frame = 1

Query: 15  TSGKIWQESSNDFWNDSDNRLNNGEVTNG---------NGGGGRSEDFELRQQRKDVEDP 74
           T GKIW++ S DFW D +  LN G               G   + E FE R+     EDP
Sbjct: 29  TVGKIWRDGSYDFWTDGEGNLNKGHNAAAVDSDRSAATTGEQQKDEGFEFRRG----EDP 88

Query: 75  PSKLIGQLLHKQKGSGEISLDMDMEMLELPQDKTLLPMVADSPMRRRHRDSLPVDEEHRG 134
           P+KLIGQ LHKQ+ SGEI LDMD+ M EL Q + L P V++SP     RD  PV      
Sbjct: 89  PTKLIGQFLHKQQASGEICLDMDLGMDEL-QSRGLTP-VSESPRVSTKRD--PVG----- 148

Query: 135 KQPQQRDLRAHGSNGEDDGGAEVLRCTSNSS-FGRDVSFQRK------------------ 194
               +RD R++ +N  DDG  EV++C+ N++   R  S   K                  
Sbjct: 149 ----RRDSRSN-TNNNDDG--EVVKCSGNNAPIQRSSSTLLKMRTRSRLSDPPTPQLPPQ 208

Query: 195 SGQVRRGLLSKAIDE---------------EDDDPFLEEDVPDKYKKGNLGVLTLLQWAS 254
           +  ++ G + K+                  E+DDPF  ED+P++Y+K  L +  +L+W S
Sbjct: 209 TADMKSGRIPKSGQMKSGFFGKSPKTQGEEEEDDPFAAEDLPEEYRKDKLSLWIVLEWLS 268

Query: 255 LILIIAALICTLAIRNWRRKKLWKLELWKWEVMILVLICGRLVSGWGIRIIVFFIERNFL 314
           LILIIA  +CTLAI + R+KKLW+L+LWKWE M+LVLICGRLVS W ++I+VFFIERNFL
Sbjct: 269 LILIIAGFVCTLAIPSLRKKKLWELQLWKWESMVLVLICGRLVSSWIVKIVVFFIERNFL 328

Query: 315 LRKRVLYFVYGIRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLVST 374
           LRKRVLYFVYG+RKAVQNCLWLGLVL+AW+FLFD+KV +   + AL  VTK+ VCLLV  
Sbjct: 329 LRKRVLYFVYGVRKAVQNCLWLGLVLLAWHFLFDEKVAKAANTKALRVVTKIFVCLLVGF 388

Query: 375 LVWLVKTLMVKVLASFFHVSTYFDRIQAALFDQYVIGTLLGPPLMEISKNEEEEERVAEE 434
           L+WLVKTL+VKVLAS FH+STYFDRIQ +LF QYVI TL GPPL+EI KNEEEEER++ E
Sbjct: 389 LLWLVKTLLVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLIEIQKNEEEEERISVE 448

Query: 435 VIKLENAGATIPPDLKATAQK-------------------GGRVIGEGITIDHLHKLSPK 494
           V K +N G     ++++ AQK                   GG    +GITID LHKL+PK
Sbjct: 449 VKKFQNPGGV---EIQSGAQKSPMKTGKSPFLSHVLSNGGGGGGENKGITIDSLHKLNPK 508

Query: 495 NVSAWNMKRLMKIIRHVTLSTLDEQIKDSITHENESTTQIKSGYEAKVAAKKIFLNVARH 554
           NVSAW MKRLM IIR+ +L+TLDEQ++D  + +++   QI+S +EAK+AA+KIF NVA+ 
Sbjct: 509 NVSAWKMKRLMNIIRNGSLTTLDEQLQDP-SLDDDKGNQIRSEFEAKLAARKIFHNVAKP 568

Query: 555 GSKYIYVEDLKRFMDEDKASKTMGLFEEARESRKISKSSLKNWVIYASRERTALASTLDD 614
           GSK+IY  D+ RF+ +D+A KT+ LFE A E+ +ISKSSLKNWV+ A RER ALA TL+D
Sbjct: 569 GSKFIYANDIMRFLPDDEALKTLSLFEGASETNRISKSSLKNWVVNAFRERRALALTLND 628

Query: 615 SKTAVKKLHRMVNILISVIILVIWLLILGIATSKFLLFATSQLVLVAFVFGNTCKTVFES 674
           +KTAV +LH+MVNI++ +IILVIWL+ILGI ++KFL+  +SQ+V+VAF+FGN CK VFES
Sbjct: 629 TKTAVNRLHKMVNIVVGIIILVIWLIILGITSTKFLVVMSSQVVVVAFIFGNMCKIVFES 688

Query: 675 IIFLFVMHPFDVGNLCEINGVQMIVKEMNILTTIFLRYDNQKIIIPNSLLATMAIHNFYR 734
           II+LFV+HPFDVG+ CEI+GVQM+V+EMNILTT+FLR+DNQK++ PNSLL T +I N+YR
Sbjct: 689 IIYLFVIHPFDVGDRCEIDGVQMVVEEMNILTTVFLRFDNQKVVYPNSLLWTKSIGNYYR 748

Query: 735 SPDMGDAVEFCLHICTPPEKIAIMRQRIISYIEGKKKHWCPAPRIFLKDVEELNRIRIAI 771
           SPDMGD +EF +HI TP EKI +++QRI SYIEGKK HW PAP I  KD+E LN +RIA+
Sbjct: 749 SPDMGDGIEFSIHITTPAEKIILIKQRITSYIEGKKDHWYPAPMIVFKDMESLNSVRIAV 808

BLAST of Lsi10G008120 vs. TAIR10
Match: AT3G14810.1 (AT3G14810.1 mechanosensitive channel of small conductance-like 5)

HSP 1 Score: 803.9 bits (2075), Expect = 8.6e-233
Identity = 448/832 (53.85%), Postives = 582/832 (69.95%), Query Frame = 1

Query: 2   SGNASLRGMKLVNTSGKIWQESSNDFWNDSDNRLNNGEVTNGNGGGGRSEDFELRQQRK- 61
           SG     G       G IW+ESS DFW D +   N+ +  + +  GG S  F  R +R+ 
Sbjct: 21  SGAVGATGSSSNAEGGNIWKESSYDFW-DGEKGKNDKKGDDEDEDGG-SFHFRQRGERRH 80

Query: 62  ---DVEDPPSKLIGQLLHKQKGSG-EISLDMDMEMLELPQDKTLLPMVADSPMRRR---- 121
              ++ DPPSKLIGQ LHKQ+ SG EISLD+++ M EL  +    P  A +  RR     
Sbjct: 81  SSAELSDPPSKLIGQFLHKQRASGDEISLDVELNMAELQSNTPPRPATASNTPRRGLTTI 140

Query: 122 HRDSLPVDEEHRGKQPQQRDLRAHGSNGEDDGG-----AEVLRCTS-------NSSFGR- 181
              S PV  + +    ++R  R       D+ G     AEVL+C S       N +  R 
Sbjct: 141 SESSSPVKTKVKADAVRRRQNRTSLGGSSDEEGRNRDEAEVLKCGSKKPMLSRNKTKSRL 200

Query: 182 --------------DVSFQRKSGQVRRGLLSKAI-----------DEEDDDPFLEEDVPD 241
                         ++   R+SG  + G L K+            +EE++DPFL+ED+P+
Sbjct: 201 QDPPTPTHPAIDKTEMKSGRRSGIFKSGFLGKSPKAGTPGRNGFEEEEEEDPFLDEDLPE 260

Query: 242 KYKKGNLGVLTLLQWASLILIIAALICTLAIRNWRRKKLWKLELWKWEVMILVLICGRLV 301
           ++K+  L     L+W SL+LI+ +L+C+L I N +RK  WKL+LWKWEV +LVLICGRLV
Sbjct: 261 EFKRDKLSFWVFLEWISLVLIVTSLVCSLTIHNLQRKTWWKLDLWKWEVTVLVLICGRLV 320

Query: 302 SGWGIRIIVFFIERNFLLRKRVLYFVYGIRKAVQNCLWLGLVLIAWNFLFDDKVQREVKS 361
           S W +RIIVF +E+NF  RKRVLYFVYG+RK+VQNCLWLGLVL+AW+FLFD KV+RE +S
Sbjct: 321 SSWIVRIIVFLVEKNFTWRKRVLYFVYGVRKSVQNCLWLGLVLLAWHFLFDKKVERETRS 380

Query: 362 NALEYVTKVLVCLLVSTLVWLVKTLMVKVLASFFHVSTYFDRIQAALFDQYVIGTLLGPP 421
            AL YVT+VLVCLLV+ ++WLVKT++VKVLAS FH+STYFDRIQ +LF QYVI TL GPP
Sbjct: 381 TALRYVTRVLVCLLVALIIWLVKTILVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPP 440

Query: 422 LMEISKNEEEEERVAEEVIKLEN-AGATIPPDLKATAQKGGRV---------------IG 481
           LMEI + EEEE++VAE+V  LE  AGA +PP LKAT +   +V                G
Sbjct: 441 LMEIQRMEEEEQQVAEDVKSLEKLAGAKLPPALKATVKSFMKVGKSPGLNRIGSKRGEDG 500

Query: 482 EGITIDHLHKLSPKNVSAWNMKRLMKIIRHVTLSTLDEQIKDSITHENESTTQIKSGYEA 541
           EGI ID L +++ KNVSAWNMKRLM II    +STLD+ ++D+ T E+E  T I+S YEA
Sbjct: 501 EGIRIDQLKRMNTKNVSAWNMKRLMNIILKGAISTLDQNMQDT-TQEDEDATHIRSEYEA 560

Query: 542 KVAAKKIFLNVARHGSKYIYVEDLKRFMDEDKASKTMGLFEEARESRKISKSSLKNWVIY 601
           K AA+KIF NV   GS+YIY+ED  RF+ E++A + M LFE A ES KISKS LKNWV+ 
Sbjct: 561 KCAARKIFHNVTEPGSRYIYLEDFLRFLCEEEAERAMALFEGASESDKISKSCLKNWVVK 620

Query: 602 ASRERTALASTLDDSKTAVKKLHRMVNILISVIILVIWLLILGIATSKFLLFATSQLVLV 661
           A RER ALA TL+D+KTAV +LHR++N++I +II++IWLLILGIAT++FLL  +SQL+LV
Sbjct: 621 AFRERRALALTLNDTKTAVDRLHRIINVVIGIIIIIIWLLILGIATTRFLLVLSSQLLLV 680

Query: 662 AFVFGNTCKTVFESIIFLFVMHPFDVGNLCEINGVQMIVKEMNILTTIFLRYDNQKIIIP 721
           AFVFGN+CKT+FE+IIFLFVMHPFDVG+ CEI+GVQ++V+EMNILTT+FLRYDNQKII P
Sbjct: 681 AFVFGNSCKTIFEAIIFLFVMHPFDVGDRCEIDGVQLVVEEMNILTTVFLRYDNQKIIYP 740

Query: 722 NSLLATMAIHNFYRSPDMGDAVEFCLHICTPPEKIAIMRQRIISYIEGKKKHWCPAPRIF 771
           NS+L T  I N+YRSPDMGDAVEFC+HI TPPEKI  ++QRI+SY++ KK +W PAP I 
Sbjct: 741 NSVLGTKPIANYYRSPDMGDAVEFCVHIATPPEKITAIKQRILSYVDNKKDYWYPAPMIV 800

BLAST of Lsi10G008120 vs. TAIR10
Match: AT1G53470.1 (AT1G53470.1 mechanosensitive channel of small conductance-like 4)

HSP 1 Score: 736.9 bits (1901), Expect = 1.3e-212
Identity = 424/837 (50.66%), Postives = 558/837 (66.67%), Query Frame = 1

Query: 16  SGKIWQESSNDFW-NDSDNRLNNGEVTNGNGGGGRSEDFELRQQRKDVE-DPPSKLIGQL 75
           S K W+ESS +FW ND  ++   GE  +G      S DF  R   K  E DPPSKLI Q 
Sbjct: 24  SEKFWRESSINFWHNDKSSKPPGGEEDDG------SFDFMRRSSEKSEEPDPPSKLINQF 83

Query: 76  LHKQKGSG-EISLDMDMEMLELPQDKTLLPMVADSPMRRRHRDSLPVDEEHR---GKQPQ 135
           L+KQK SG EISLDM+  M EL Q  T+ P+ + +        + PV   +R   G   +
Sbjct: 84  LNKQKASGDEISLDMEANMPEL-QKNTVPPLSSTAVSGSASPVTAPVTASYRNGTGDAIR 143

Query: 136 QRDLRA-------HGSNGEDD----GGAEVLRCTSNSSFGRDVSFQR------------- 195
           +R  R         G + ED+     G+EV++CTSN S  R  +  +             
Sbjct: 144 RRQNRVTLSPSVKDGDSSEDEENRVDGSEVVKCTSNRSTMRTKTLMKMKTRSRLMDPPTP 203

Query: 196 -----------KSGQVRRGLLSKAID------------EEDDDPFLEEDVPDKYKKGNLG 255
                      +SG +  G   +               EE++DPF EED+P+  +K  + 
Sbjct: 204 TYPDMVSGRTPRSGNLNPGFSGRNTKPGTPNQGGSKDLEEEEDPFSEEDLPEGLRKEKIC 263

Query: 256 VLTLLQWASLILIIAALICTLAIRNWRRKKLWKLELWKWEVMILVLICGRLVSGWGIRII 315
           V  +++W  LILIIA+LIC+L I   R K LW L LWKWEVM+LVLICGRLVS W +++ 
Sbjct: 264 VWVIIEWIFLILIIASLICSLVIPYLRGKTLWDLALWKWEVMVLVLICGRLVSSWIVKLF 323

Query: 316 VFFIERNFLLRKRVLYFVYGIRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNALEYVTK 375
           V+F+E NFL RK+VLYFVYGIRK VQNCLWLGLVLIAW+FLFD KV+RE++S  L+YVTK
Sbjct: 324 VYFVESNFLWRKKVLYFVYGIRKPVQNCLWLGLVLIAWHFLFDKKVEREMRSTVLKYVTK 383

Query: 376 VLVCLLVSTLVWLVKTLMVKVLASFFHVSTYFDRIQAALFDQYVIGTLLGPPLMEISKNE 435
           VL+CLLV+ ++WL+KTL+VKVLAS FH+STYFDRIQ +LF QYVI TL GPP +EI    
Sbjct: 384 VLICLLVAVIIWLIKTLLVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPRIEI---H 443

Query: 436 EEEERVAEEVIKLENAGATI----------PPDL---KATAQKGGRVIGEGITIDHLH-- 495
            EEE+VA +V   E  G  +          PP +       QK    +G+   +      
Sbjct: 444 IEEEKVANDVKTFEIVGRKLSPLGPKAVSSPPQVTVGSGRLQKSPSRVGKSPVLSRSGSK 503

Query: 496 --------------KLSPKNVSAWNMKRLMKIIRHVTLSTLDEQIKDSITHENESTTQIK 555
                         +++ KNVSAW MK+LM +I+  TLSTLDEQI+D+ T E++  TQI+
Sbjct: 504 KEGGEEGIRIDHLQRMNTKNVSAWKMKKLMNVIKKGTLSTLDEQIQDTTTQEDDKATQIR 563

Query: 556 SGYEAKVAAKKIFLNVARHGSKYIYVEDLKRFMDEDKASKTMGLFEEARESRKISKSSLK 615
           S +EAK+AA+KIF NVA  GS+YIY+ED  RF+ ED++ + M LFE A E  KISKS LK
Sbjct: 564 SEFEAKLAARKIFQNVAEPGSRYIYMEDFMRFLSEDESERAMDLFEGASECHKISKSCLK 623

Query: 616 NWVIYASRERTALASTLDDSKTAVKKLHRMVNILISVIILVIWLLILGIATSKFLLFATS 675
           NWV+ A RER ALA TL+D+KTAV +LHR+V++L+S++IL+IWLLILGIAT+KFLL  +S
Sbjct: 624 NWVVNAFRERRALALTLNDTKTAVNRLHRIVDVLVSIVILIIWLLILGIATTKFLLVISS 683

Query: 676 QLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGNLCEINGVQMIVKEMNILTTIFLRYDNQ 735
           QL+LV FVFGN+CKT+FE++IF+FVMHPFDVG+ CEI+GVQMIV+EMNILTT+FLR+DNQ
Sbjct: 684 QLLLVVFVFGNSCKTIFEAVIFVFVMHPFDVGDRCEIDGVQMIVEEMNILTTVFLRFDNQ 743

Query: 736 KIIIPNSLLATMAIHNFYRSPDMGDAVEFCLHICTPPEKIAIMRQRIISYIEGKKKHWCP 771
           KI+ PNSLL T  I N+YRSPDM DA+EF +HI TPPEK   +RQRI+SY++ KK HW P
Sbjct: 744 KIVYPNSLLGTKPIANYYRSPDMQDAIEFFVHIATPPEKTTALRQRILSYVDNKKDHWHP 803

BLAST of Lsi10G008120 vs. TAIR10
Match: AT2G17000.1 (AT2G17000.1 Mechanosensitive ion channel family protein)

HSP 1 Score: 719.9 bits (1857), Expect = 1.6e-207
Identity = 374/621 (60.23%), Postives = 487/621 (78.42%), Query Frame = 1

Query: 167 SGQVRRGLLSKAID--EEDDDPFLEEDVPDKYKKGNLGVLTLLQWASLILIIAALICTLA 226
           S Q+R GLL +  D  EE+DD   EEDVP +Y+K  +  +TLLQW SLI ++ AL+ +L 
Sbjct: 193 SDQLRSGLLGRHSDDIEEEDDSSAEEDVPVEYRKLKMDAITLLQWMSLIALVVALVLSLG 252

Query: 227 IRNWRRKKLWKLELWKWEVMILVLICGRLVSGWGIRIIVFFIERNFLLRKRVLYFVYGIR 286
           +  WR   LW L LWKWEV++LVLICGRLVSG GIRIIVFFIERNFLLRKRVLYFVYG++
Sbjct: 253 LHTWRNATLWSLHLWKWEVVLLVLICGRLVSGCGIRIIVFFIERNFLLRKRVLYFVYGVK 312

Query: 287 KAVQNCLWLGLVLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLVSTLVWLVKTLMVKVL 346
            AVQNCLWLGLVL+AW+FLFD KV++E +S+ L  ++K+LVC L+ST++WL+KTL+VKVL
Sbjct: 313 TAVQNCLWLGLVLLAWHFLFDKKVEKETQSDVLLLMSKILVCFLLSTVLWLIKTLVVKVL 372

Query: 347 ASFFHVSTYFDRIQAALFDQYVIGTLLGPPLMEISKNEEEEERVAEEVIKLENAGATIPP 406
           AS FHVSTYFDRIQ ALF  Y+I TL GPP++E+S+ EEEE+R  +E+ K++  GA + P
Sbjct: 373 ASSFHVSTYFDRIQEALFHHYLIETLSGPPMLELSRIEEEEDRTQDEIYKMQKGGADLSP 432

Query: 407 DLKATA---QKGGRVIG-------------EGITIDHLHKLSPKNVSAWNMKRLMKIIRH 466
           +L + A   +K G  +               GIT+D LHK++ KNVSAWNMKRLMKI+R+
Sbjct: 433 ELCSAAFPQEKSGSTMNMKFSPIIPKTGSDNGITMDDLHKMNQKNVSAWNMKRLMKIVRN 492

Query: 467 VTLSTLDEQIKDSITHENESTTQIKSGYEAKVAAKKIFLNVARHGSKYIYVEDLKRFMDE 526
           V+LSTLDEQ   + T E+EST QI+S  EAK AA+KIF NVA+ G+K+IY+EDL RF+  
Sbjct: 493 VSLSTLDEQALQN-TCEDESTRQIRSEKEAKAAARKIFKNVAQPGTKHIYLEDLMRFLRV 552

Query: 527 DKASKTMGLFEEARESRKISKSSLKNWVIYASRERTALASTLDDSKTAVKKLHRMVNILI 586
           D+A KTM LFE A  ++KI+KS+LKNW++ A RER ALA TL+D+KTAV KLH M++ L 
Sbjct: 553 DEAMKTMCLFEGALVTKKITKSALKNWLVNAFRERRALALTLNDTKTAVNKLHHMISFLT 612

Query: 587 SVIILVIWLLILGIATSKFLLFATSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGNLC 646
           +++I+VIWL++L IATSK+LLF TSQ+VL+AF+FGN+ KTVFESIIFLF++HP+DVG+  
Sbjct: 613 AIVIIVIWLILLEIATSKYLLFLTSQVVLLAFMFGNSLKTVFESIIFLFIIHPYDVGDRL 672

Query: 647 EINGVQMIVKEMNILTTIFLRYDNQKIIIPNSLLATMAIHNFYRSPDMGDAVEFCLHICT 706
            I+ V+M+V+EMNILTT+FLR DN KI+ PN LL   AIHN+ RSPDMGD V  C+HI T
Sbjct: 673 LIDTVEMVVEEMNILTTVFLRADNLKIVYPNILLWQKAIHNYNRSPDMGDEVTCCVHITT 732

Query: 707 PPEKIAIMRQRIISYIEGKKKHWCPAPRIFLKDVEELNRIRIAICLTHRMNHQDMGKRWT 766
           PPEKIA ++QRI SYI+ K ++W P   + +KDVE+LN +RIAI L H++NHQ+MG+R+T
Sbjct: 733 PPEKIAAIKQRISSYIDSKPEYWYPKADVIVKDVEDLNIVRIAIWLCHKINHQNMGERFT 792

Query: 767 RRALLVEELVKIFQEIDLQYR 770
           RRALL+EE++KI  E+D+QYR
Sbjct: 793 RRALLIEEVIKILLELDIQYR 812

BLAST of Lsi10G008120 vs. TAIR10
Match: AT2G17010.1 (AT2G17010.1 Mechanosensitive ion channel family protein)

HSP 1 Score: 438.0 bits (1125), Expect = 1.2e-122
Identity = 215/342 (62.87%), Postives = 293/342 (85.67%), Query Frame = 1

Query: 428 KLSPKNVSAWNMKRLMKIIRHVTLSTLDEQIKDSITHENESTTQIKSGYEAKVAAKKIFL 487
           +++ KN+SAWNMKRLMKI+R+V+L+TLDEQ+ +S T+E+EST QI+S  EAK AA+KIF 
Sbjct: 410 RMNHKNISAWNMKRLMKIVRNVSLTTLDEQMLES-TYEDESTRQIRSEKEAKAAARKIFK 469

Query: 488 NVARHGSKYIYVEDLKRFMDEDKASKTMGLFEEARESRKISKSSLKNWVIYASRERTALA 547
           NV + G+KYIY+EDL RF+ ED+A KTMGLFE A E+++ISKS+LKNW++ A RER ALA
Sbjct: 470 NVEQRGAKYIYLEDLMRFLREDEAMKTMGLFEGAPENKRISKSALKNWLVNAFRERRALA 529

Query: 548 STLDDSKTAVKKLHRMVNILISVIILVIWLLILGIATSKFLLFATSQLVLVAFVFGNTCK 607
            TL+D+KTAV KLH M+NI+ +++I+VIWL++L IA+SK LLF +SQ+VL+AF+FGNT K
Sbjct: 530 LTLNDTKTAVNKLHHMINIVTAIVIVVIWLVLLEIASSKVLLFVSSQVVLLAFIFGNTVK 589

Query: 608 TVFESIIFLFVMHPFDVGNLCEINGVQMIVKEMNILTTIFLRYDNQKIIIPNSLLATMAI 667
           TVFESIIFLF++HP+DVG+ CEI+ VQ++V+EMNILTT+FLRYDN KI+ PNSLL   +I
Sbjct: 590 TVFESIIFLFIVHPYDVGDRCEIDSVQLVVEEMNILTTVFLRYDNLKIMYPNSLLWQKSI 649

Query: 668 HNFYRSPDMGDAVEFCLHICTPPEKIAIMRQRIISYIEGKKKHWCPAPRIFLKDVEELNR 727
           +N+YRSPDMGDA+EFC+HI TP EKI++++QRI +YI+ K ++W P  +I +KD+E+L+ 
Sbjct: 650 NNYYRSPDMGDAIEFCVHITTPLEKISVIKQRISNYIDNKPEYWYPQAKIIVKDLEDLHI 709

Query: 728 IRIAICLTHRMNHQDMGKRWTRRALLVEELVKIFQEIDLQYR 770
           +R+AI   HR+NHQDM +RWTRRA+LVEE++KI  E+D+Q+R
Sbjct: 710 VRLAIWPCHRINHQDMAERWTRRAVLVEEVIKILLELDIQHR 750

BLAST of Lsi10G008120 vs. NCBI nr
Match: gi|659095000|ref|XP_008448342.1| (PREDICTED: mechanosensitive ion channel protein 6-like [Cucumis melo])

HSP 1 Score: 1127.9 bits (2916), Expect = 0.0e+00
Identity = 624/841 (74.20%), Postives = 677/841 (80.50%), Query Frame = 1

Query: 5   ASLRGMKLVNTSGKIWQESSNDFWNDSDNRLNNGE----VTNGNGGGGRSEDFE------ 64
           +SLR   LVN SG+IW+ESS DFWNDSDNR NNGE     T         +D E      
Sbjct: 64  SSLRATDLVNGSGRIWRESSYDFWNDSDNRRNNGEGGARTTEDFEFRQHRKDVEDPPSKL 123

Query: 65  ----LRQQRKDVE---DPPSKLIGQLLHKQKGSGEISLDMDMEMLELPQDKTLLPMVADS 124
               L +Q+   E   D   +++     K   S      M     EL      +  ++++
Sbjct: 124 IGQFLHKQKASGEMSLDMDMEMLELPQDKTPLSTVAESPMRRSSRELKVSFESISEISEN 183

Query: 125 P-MRRRHRDSLPVDEEHRGKQPQQRDLRAHGSNGEDDGGAEVLRCTSNSSFGRDVSFQRK 184
             MRRRHRDS P+DEEHRG+QP+Q D RAHGSNGEDDG AEVLRC+SNSSF RDVSFQRK
Sbjct: 184 DSMRRRHRDS-PLDEEHRGQQPRQCDRRAHGSNGEDDGAAEVLRCSSNSSFQRDVSFQRK 243

Query: 185 S----------------------------GQVRRGLLSKAIDEEDDDPFLEEDVPDKYKK 244
           S                            GQVR GL+SK +DEEDDDPFLEED+PD+YKK
Sbjct: 244 SSLLRAKTKSRLLDPPEHQDRRSGRVPKSGQVRSGLISKVLDEEDDDPFLEEDLPDEYKK 303

Query: 245 GNLGVLTLLQWASLILIIAALICTLAIRNWRRKKLWKLELWKWEVMILVLICGRLVSGWG 304
            NLGVLTLLQWASLILIIAAL+CTL IR WRRKKLWKLE+WKWEVMILVLICGRLVSGWG
Sbjct: 304 ANLGVLTLLQWASLILIIAALVCTLTIRYWRRKKLWKLEVWKWEVMILVLICGRLVSGWG 363

Query: 305 IRIIVFFIERNFLLRKRVLYFVYGIRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNALE 364
           IR+IVFFIERNFLLRKRVLYFVYG+RKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNALE
Sbjct: 364 IRVIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNALE 423

Query: 365 YVTKVLVCLLVSTLVWLVKTLMVKVLASFFHVSTYFDRIQAALFDQYVIGTLLGPPLMEI 424
           YVTKVLVCLL+STLVWLVKTLMVKVLAS FHVSTYFDRIQ ALF+QYVI TL GPPL+EI
Sbjct: 424 YVTKVLVCLLISTLVWLVKTLMVKVLASSFHVSTYFDRIQDALFNQYVIETLSGPPLIEI 483

Query: 425 SKNEEEEERVAEEVIKLENAGATIPPDLKATA----QKGGRVIG---------------- 484
            KNEEEEER+AEEV+KL+NAGATIPPDLKATA    QKGGRVIG                
Sbjct: 484 QKNEEEEERLAEEVMKLQNAGATIPPDLKATAFSTAQKGGRVIGSGGLQKSPRGRSGKLS 543

Query: 485 ---------EGITIDHLHKLSPKNVSAWNMKRLMKIIRHVTLSTLDEQIKDSITHENEST 544
                    EGITIDHLHKLSPKNVSAWNMKRLM I+RH TLSTLDEQIKD+  HE+EST
Sbjct: 544 RTLSKKGGDEGITIDHLHKLSPKNVSAWNMKRLMNIVRHGTLSTLDEQIKDT-AHEDEST 603

Query: 545 TQIKSGYEAKVAAKKIFLNVARHGSKYIYVEDLKRFMDEDKASKTMGLFEEARESRKISK 604
           TQIKS YEAKVAAKKIFLNVAR+GSKYIY+EDL RFM++D+ASKTMGLFE A ESRKISK
Sbjct: 604 TQIKSEYEAKVAAKKIFLNVARNGSKYIYLEDLMRFMEKDEASKTMGLFEGACESRKISK 663

Query: 605 SSLKNWVIYASRERTALASTLDDSKTAVKKLHRMVNILISVIILVIWLLILGIATSKFLL 664
           SSLKNWV+ A RER ALA TL+D+KTAV KLHRMVNIL+SVIILVIWLLILGIATSKFLL
Sbjct: 664 SSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLILGIATSKFLL 723

Query: 665 FATSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGNLCEINGVQMIVKEMNILTTIFLR 724
           F TSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVG+ CEI+GVQMIV+EMNILTTIFLR
Sbjct: 724 FVTSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTIFLR 783

Query: 725 YDNQKIIIPNSLLATMAIHNFYRSPDMGDAVEFCLHICTPPEKIAIMRQRIISYIEGKKK 771
           YDNQKII PNS+LAT AIHNFYRSPDMGD VEFCLHI TPPEKIAIMRQRI+SYIEGKK+
Sbjct: 784 YDNQKIIFPNSVLATKAIHNFYRSPDMGDGVEFCLHISTPPEKIAIMRQRIVSYIEGKKE 843

BLAST of Lsi10G008120 vs. NCBI nr
Match: gi|449444568|ref|XP_004140046.1| (PREDICTED: mechanosensitive ion channel protein 6-like [Cucumis sativus])

HSP 1 Score: 1122.5 bits (2902), Expect = 0.0e+00
Identity = 623/841 (74.08%), Postives = 676/841 (80.38%), Query Frame = 1

Query: 5   ASLRGMKLVNTSGKIWQESSNDFWNDSDNRLNNGE----VTNGNGGGGRSEDFE------ 64
           ++LR   LVN SG IW+ESS DFWNDSDNR N GE     T+        +D E      
Sbjct: 64  STLRATDLVNGSGTIWRESSYDFWNDSDNRRNYGEGGAKTTDDFEFRQHRKDVEDPPSKL 123

Query: 65  ----LRQQRKDVE---DPPSKLIGQLLHKQKGSGEISLDMDMEMLELPQDKTLLPMVADS 124
               L +Q+   E   D   +++     K   S      M     EL      +  ++++
Sbjct: 124 IGQFLHKQKASGEMSLDMDMEMLELPQDKTPLSTVAESPMRRSSRELKVSFESISEISEN 183

Query: 125 P-MRRRHRDSLPVDEEHRGKQPQQRDLRAHGSNGEDDGGAEVLRCTSNSSFGRDVSFQRK 184
             MRRRHRDS P+DEEHRG+QP+Q D RAHGSNGEDDG AEVLRC+SNS F RDVSFQRK
Sbjct: 184 DSMRRRHRDS-PLDEEHRGQQPRQCDRRAHGSNGEDDGAAEVLRCSSNSFFQRDVSFQRK 243

Query: 185 S----------------------------GQVRRGLLSKAIDEEDDDPFLEEDVPDKYKK 244
           S                            GQVR GL+SKA+DEEDDDPFLEED+PD+YKK
Sbjct: 244 SSLLRAKTKSRLLDPPEHQDRRSGRVPKSGQVRSGLISKALDEEDDDPFLEEDLPDEYKK 303

Query: 245 GNLGVLTLLQWASLILIIAALICTLAIRNWRRKKLWKLELWKWEVMILVLICGRLVSGWG 304
            NLGVLTLLQWASLILIIAAL+CTL IR WRRKKLWKLE+WKWEVMILVLICGRLVSGWG
Sbjct: 304 ANLGVLTLLQWASLILIIAALVCTLTIRYWRRKKLWKLEVWKWEVMILVLICGRLVSGWG 363

Query: 305 IRIIVFFIERNFLLRKRVLYFVYGIRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNALE 364
           IR+IVFFIERNFLLRKRVLYFVYG+RKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNALE
Sbjct: 364 IRVIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNALE 423

Query: 365 YVTKVLVCLLVSTLVWLVKTLMVKVLASFFHVSTYFDRIQAALFDQYVIGTLLGPPLMEI 424
           YVTKVLVCLLVSTLVWLVKTLMVKVLAS FHVSTYFDRIQ ALF+QYVI TL GPPL+EI
Sbjct: 424 YVTKVLVCLLVSTLVWLVKTLMVKVLASSFHVSTYFDRIQDALFNQYVIETLSGPPLIEI 483

Query: 425 SKNEEEEERVAEEVIKLENAGATIPPDLKATA----QKGGRVIG---------------- 484
            KNEEEEER+AEEVIKL+NAGATIPPDLKATA    QKGGRVIG                
Sbjct: 484 QKNEEEEERLAEEVIKLQNAGATIPPDLKATAFSTAQKGGRVIGSGGLQKSPRGRSGKLS 543

Query: 485 ---------EGITIDHLHKLSPKNVSAWNMKRLMKIIRHVTLSTLDEQIKDSITHENEST 544
                    EGITIDHLHKLSPKNVSAWNMKRLM I+RH TLSTLDEQIKD+  HE+EST
Sbjct: 544 RTLSKKGGDEGITIDHLHKLSPKNVSAWNMKRLMNIVRHGTLSTLDEQIKDT-AHEDEST 603

Query: 545 TQIKSGYEAKVAAKKIFLNVARHGSKYIYVEDLKRFMDEDKASKTMGLFEEARESRKISK 604
           T+IKS YEAKVAAKKIFLNVAR+GSKYIY+EDL RFM++D+ASKTMGLFE A ESRKISK
Sbjct: 604 TEIKSEYEAKVAAKKIFLNVARNGSKYIYLEDLMRFMEKDEASKTMGLFEGACESRKISK 663

Query: 605 SSLKNWVIYASRERTALASTLDDSKTAVKKLHRMVNILISVIILVIWLLILGIATSKFLL 664
           SSLKNWV+ A RER ALA TL+D+KTAV KLHRMVNIL+SVIILVIWLLILGIATSKFLL
Sbjct: 664 SSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLILGIATSKFLL 723

Query: 665 FATSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGNLCEINGVQMIVKEMNILTTIFLR 724
           F TSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVG+ CEI+GVQMIV+EMNILTTIFLR
Sbjct: 724 FVTSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTIFLR 783

Query: 725 YDNQKIIIPNSLLATMAIHNFYRSPDMGDAVEFCLHICTPPEKIAIMRQRIISYIEGKKK 771
           YDNQKII PNS+LAT AIHNFYRSPDMGD +EFCLHI TPPEKIAIMRQRIISYIEGKK+
Sbjct: 784 YDNQKIIFPNSVLATKAIHNFYRSPDMGDGIEFCLHISTPPEKIAIMRQRIISYIEGKKE 843

BLAST of Lsi10G008120 vs. NCBI nr
Match: gi|590611003|ref|XP_007021979.1| (Mechanosensitive channel of small conductance-like 6, putative [Theobroma cacao])

HSP 1 Score: 941.0 bits (2431), Expect = 1.3e-270
Identity = 520/836 (62.20%), Postives = 621/836 (74.28%), Query Frame = 1

Query: 17  GKIWQESSNDFWNDSD---------NRLNNGEVTNGNGGGGRSEDFE-LRQQRKDVEDPP 76
           GKIW+ESS DFW DS+         N   NG  ++ N     SE F+ +R ++  +EDPP
Sbjct: 37  GKIWRESSYDFWKDSEKISSNWKKENANMNGTGSSANNSSRESEGFDFMRSKQAAIEDPP 96

Query: 77  SKLIGQLLHKQKGSGEISLDMDMEMLELPQDK----TLLPMVADSPMRRRHRDSLPVDEE 136
           SKLIGQ LHKQK SGEISLDMD+EM EL Q+     TLLP VA+SP            E 
Sbjct: 97  SKLIGQFLHKQKASGEISLDMDLEMDELQQEPPHHGTLLPTVAESPSPSAAAVPRVSFEN 156

Query: 137 HRGKQPQQRDLRAHGSNGEDDG--GAEVLRCTSNSSFGRDV--SFQRKS----------- 196
           +  ++ Q +   + G     D   G  V++C+SNSSF R    SFQRKS           
Sbjct: 157 NAVRRRQSKGSASPGKEESRDSKEGDGVVKCSSNSSFKRSEGGSFQRKSSLLATKTKSRL 216

Query: 197 ---------------------GQVRR-GLLSKAIDEEDDDPFLEEDVPDKYKKGNLGVLT 256
                                GQ+ R G L K+++EE+DDP LEED+PD+YKK  L VL 
Sbjct: 217 MDPPTPEKGEPRSAKATVGKSGQIMRSGFLGKSMEEEEDDPLLEEDLPDEYKKDKLSVLV 276

Query: 257 LLQWASLILIIAALICTLAIRNWRRKKLWKLELWKWEVMILVLICGRLVSGWGIRIIVFF 316
           LL+W SLILI+AA +C+L I   R K+LW L LWKWEV++LVLICGRLVSGW IRIIVFF
Sbjct: 277 LLEWLSLILIVAAFVCSLTIPYLRAKRLWSLMLWKWEVLVLVLICGRLVSGWIIRIIVFF 336

Query: 317 IERNFLLRKRVLYFVYGIRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNALEYVTKVLV 376
           IERNFLLRKRVLYFVYG+RKAVQNCLWLGLVLIAW++LFD KVQRE KS  L YVTKVLV
Sbjct: 337 IERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWHYLFDKKVQRETKSEFLRYVTKVLV 396

Query: 377 CLLVSTLVWLVKTLMVKVLASFFHVSTYFDRIQAALFDQYVIGTLLGPPLMEISKNEEEE 436
           CL+V  ++WLVKTL+VKVLAS FHVSTYFDRIQ +LF+QYV+ TL GPPL+EI + EEEE
Sbjct: 397 CLVVGVMLWLVKTLLVKVLASSFHVSTYFDRIQDSLFNQYVVETLSGPPLIEIQRAEEEE 456

Query: 437 ERVAEEVIKLENAGATIPPDLKAT---AQKGGRVIG------------------------ 496
           ER+A EV  L+ AGATIPP LK +   +   G+VIG                        
Sbjct: 457 ERIANEVKNLQKAGATIPPGLKTSTLSSPHSGKVIGSGRIQKSPRGKSPMLSRMLSSEKG 516

Query: 497 ----EGITIDHLHKLSPKNVSAWNMKRLMKIIRHVTLSTLDEQIKDSITHENESTTQIKS 556
               +GITIDHLHKL+ KNVSAWNMKRLM IIRH  LSTLDEQI+DS THE+ES TQI+S
Sbjct: 517 EKDKKGITIDHLHKLNHKNVSAWNMKRLMNIIRHGALSTLDEQIQDS-THEDESATQIRS 576

Query: 557 GYEAKVAAKKIFLNVARHGSKYIYVEDLKRFMDEDKASKTMGLFEEARESRKISKSSLKN 616
            YEAKVAA+KIF NVA+ GSKYIY+ED++RF+ ED+A KTM LFE A ESR+ISK +LKN
Sbjct: 577 EYEAKVAARKIFQNVAKPGSKYIYLEDIERFLQEDEAFKTMSLFEGASESRRISKKALKN 636

Query: 617 WVIYASRERTALASTLDDSKTAVKKLHRMVNILISVIILVIWLLILGIATSKFLLFATSQ 676
           WV+ A RER ALA TL+D+KTAV +LHRMVN+L+ +II+VIWLLILGIA+SK L+F +SQ
Sbjct: 637 WVVNAFRERRALALTLNDTKTAVNRLHRMVNVLVGIIIVVIWLLILGIASSKVLVFISSQ 696

Query: 677 LVLVAFVFGNTCKTVFESIIFLFVMHPFDVGNLCEINGVQMIVKEMNILTTIFLRYDNQK 736
           L+LVAF+FGNTCKTVFE+IIFLFVMHPFDVG+ CEI+GVQM+V+EMNILTT+FLRYDNQK
Sbjct: 697 LLLVAFIFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMVVEEMNILTTVFLRYDNQK 756

Query: 737 IIIPNSLLATMAIHNFYRSPDMGDAVEFCLHICTPPEKIAIMRQRIISYIEGKKKHWCPA 771
           IIIPNS+LAT AI+N+YRSPDMGDAVEFC+H+ TP EKI +M+QRI+SYIE K  HW PA
Sbjct: 757 IIIPNSVLATKAINNYYRSPDMGDAVEFCIHVKTPAEKIGLMKQRILSYIEHKSDHWYPA 816

BLAST of Lsi10G008120 vs. NCBI nr
Match: gi|702276066|ref|XP_010044420.1| (PREDICTED: mechanosensitive ion channel protein 6-like [Eucalyptus grandis])

HSP 1 Score: 930.2 bits (2403), Expect = 2.3e-267
Identity = 513/822 (62.41%), Postives = 610/822 (74.21%), Query Frame = 1

Query: 18  KIWQESSNDFWNDSDNRLNNGEVTNGNGGGGRS---------EDFELR---QQRKDVEDP 77
           KIW+ SS DFW D        E   G+GGGG S         ++F+ R   ++    EDP
Sbjct: 7   KIWRGSSYDFWKD--------EEVGGSGGGGASGAGPRVSDGDEFDFRKGGEKGSGSEDP 66

Query: 78  PSKLIGQLLHKQKGSGEISLDMDMEMLELPQDK--------TLLPMVADSPMRRRHRDSL 137
           PSKLIGQ LHKQ+ SG+ +LD+D+EM EL QD+          LP + +SPM+ R++ S 
Sbjct: 67  PSKLIGQFLHKQRASGDFTLDIDLEMQELKQDEDNDDGDTFAPLPPLEESPMKSRNKVSF 126

Query: 138 --PVDEEHRGKQPQQRDLRAHGSNGEDDGGAEVLRCTSNSSFGRDVSFQRKS----GQVR 197
             P   E R  +      +     G +DG   VLRCTSNSSF  + SF+RKS    G V+
Sbjct: 127 GEPGSSEGRTARVSVESQQRQQDEGSEDG--PVLRCTSNSSFQSNSSFKRKSRLLTGAVK 186

Query: 198 -------------------------RGLLSKAIDEEDDDPFLEEDVPDKYKKGNLGVLTL 257
                                    +G   +  DE+++DPF  ED+PD+YKKG L ++ L
Sbjct: 187 NKSRLLDPPDHADRRSGRVSKLGTPKGTPKRGGDEDEEDPFAGEDLPDQYKKGRLELVVL 246

Query: 258 LQWASLILIIAALICTLAIRNWRRKKLWKLELWKWEVMILVLICGRLVSGWGIRIIVFFI 317
           L+W SL+LI+AAL+C+L I   R+KKLW L LWKWEVM+LVLICGRLVS WGIRI VFFI
Sbjct: 247 LEWVSLVLIVAALVCSLTIPYLRKKKLWDLMLWKWEVMVLVLICGRLVSSWGIRIAVFFI 306

Query: 318 ERNFLLRKRVLYFVYGIRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNALEYVTKVLVC 377
           ERNF+LRKRVLYFVYG+RKAVQNCLWLGLVL+AW+FLFD KVQRE  S AL+ VTK+LVC
Sbjct: 307 ERNFVLRKRVLYFVYGLRKAVQNCLWLGLVLLAWHFLFDKKVQRETNSEALQVVTKILVC 366

Query: 378 LLVSTLVWLVKTLMVKVLASFFHVSTYFDRIQAALFDQYVIGTLLGPPLMEISKNEEEEE 437
           LLV TL+WLVKTL+VKVLAS FHVSTYFDRIQ +LF+QYVI TL GPPL+E+ K EEEEE
Sbjct: 367 LLVGTLLWLVKTLIVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLIEMRKAEEEEE 426

Query: 438 RVAEEVIKLENAGATIPPDLKAT---AQKGGRVIG---------------EGITIDHLHK 497
           ++A EV KL+ AGA IPPDL A    A+  GRVIG               EGITIDHLHK
Sbjct: 427 KIAVEVEKLKKAGAVIPPDLNAAVLPAKGSGRVIGSGIVQKSPRGKDKGEEGITIDHLHK 486

Query: 498 LSPKNVSAWNMKRLMKIIRHVTLSTLDEQIKDSITHENESTTQIKSGYEAKVAAKKIFLN 557
           LSPKNVSAWNMKRLM ++R+  L+TLDEQ+    +HE+ES+ QI S  EA+ AAKKIF N
Sbjct: 487 LSPKNVSAWNMKRLMNLVRYGNLTTLDEQLS---SHEDESSNQINSEGEARAAAKKIFQN 546

Query: 558 VARHGSKYIYVEDLKRFMDEDKASKTMGLFEEARESRKISKSSLKNWVIYASRERTALAS 617
           VAR GSK+IY+EDL RFM ED+A KTM LFE A ES+KISKSSLKNWV+ A RER ALA 
Sbjct: 547 VARQGSKHIYLEDLMRFMPEDEAVKTMSLFEGASESQKISKSSLKNWVVNAFRERRALAL 606

Query: 618 TLDDSKTAVKKLHRMVNILISVIILVIWLLILGIATSKFLLFATSQLVLVAFVFGNTCKT 677
           TLDD+KTAV KLHRMVNIL+ ++I+VIWLLILGIAT KFLL  +SQL+LV F+FGN+CK 
Sbjct: 607 TLDDTKTAVNKLHRMVNILVGILIVVIWLLILGIATKKFLLLISSQLLLVVFIFGNSCKM 666

Query: 678 VFESIIFLFVMHPFDVGNLCEINGVQMIVKEMNILTTIFLRYDNQKIIIPNSLLATMAIH 737
            FE+IIFLFVMHPFDVG+ CEI+GVQM+V+EMNILTTIFLRYDNQKIIIPNSLLAT AI 
Sbjct: 667 TFEAIIFLFVMHPFDVGDRCEIDGVQMVVEEMNILTTIFLRYDNQKIIIPNSLLATKAIS 726

Query: 738 NFYRSPDMGDAVEFCLHICTPPEKIAIMRQRIISYIEGKKKHWCPAPRIFLKDVEELNRI 771
           N+YRSPDMGDAVEFC+HI TP EKIA+M+QRI SYIE K  HW PAP I  KDVEELN++
Sbjct: 727 NYYRSPDMGDAVEFCVHISTPTEKIAVMKQRITSYIENKNDHWYPAPMIIFKDVEELNKL 786

BLAST of Lsi10G008120 vs. NCBI nr
Match: gi|568848026|ref|XP_006477826.1| (PREDICTED: mechanosensitive ion channel protein 6 [Citrus sinensis])

HSP 1 Score: 909.4 bits (2349), Expect = 4.1e-261
Identity = 498/860 (57.91%), Postives = 611/860 (71.05%), Query Frame = 1

Query: 1   MSGNASLRGMKLVNTSGKIWQESSNDFWNDSDNRLNNGEVTNGNGGGGRSEDFELRQQRK 60
           + GN       + + + KIW+ SS DFW D     NN +    +GG G  E F+  Q   
Sbjct: 15  VDGNKPNNSSSVSSNNSKIWRGSSYDFWEDRGKMDNNVK----SGGVGGEEGFDFMQHGP 74

Query: 61  DVEDPPSKLIGQLLHKQKGSGEISLDMDMEMLELPQDKTL-------------------- 120
            +EDPPS LIGQ LHKQK SGEISLDMD+EM EL    +                     
Sbjct: 75  SMEDPPSVLIGQFLHKQKASGEISLDMDLEMDELQHQASNKNNNCGSSSGININSNTKST 134

Query: 121 --LPMVADSP----------MRRRHRDSLPVDEEHRGKQPQQRDLRAHGSNGEDDGGAEV 180
             LP V++SP          ++RRH +S      +    PQ+            D   EV
Sbjct: 135 QGLPTVSESPTAVNRVSFESLKRRHSNST---NNNYKDSPQK------------DSEGEV 194

Query: 181 LRCTSNSSFGRDVSFQRKS------------------------------GQVRRGLLSKA 240
           ++CTSN SF R+VSF +KS                              GQ++ G + K 
Sbjct: 195 VKCTSNKSFDRNVSFNKKSALLMTKTKSRLMDLPPERIEPKSGRVVGRSGQLKSGFIGKN 254

Query: 241 IDEEDDDPFLEEDVPDKYKKGNLGVLTLLQWASLILIIAALICTLAIRNWRRKKLWKLEL 300
           +DEE++DP LEED+P++YKK  + +  LL+W SLILII AL+C+L I  +++KKLWKL L
Sbjct: 255 VDEEEEDPLLEEDLPEEYKKEKISIWVLLEWFSLILIIGALVCSLTIDYFKKKKLWKLGL 314

Query: 301 WKWEVMILVLICGRLVSGWGIRIIVFFIERNFLLRKRVLYFVYGIRKAVQNCLWLGLVLI 360
           WKWE++ILVLICGRLVS W +RIIVF IERNFLLRKRVLYFVYG+RKAVQNCLWLGLVLI
Sbjct: 315 WKWELLILVLICGRLVSSWIVRIIVFCIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLI 374

Query: 361 AWNFLFDDKVQREVKSNALEYVTKVLVCLLVSTLVWLVKTLMVKVLASFFHVSTYFDRIQ 420
           AW+ LFD +V+RE  S  L+Y TK+L+CL V  ++WLVKTL+VKVLAS FHVSTYFDRIQ
Sbjct: 375 AWHCLFDQRVERETNSKVLKYATKILICLWVGVMLWLVKTLLVKVLASSFHVSTYFDRIQ 434

Query: 421 AALFDQYVIGTLLGPPLMEISKNEEEEERVAEEVIKLENAGATIPPDLKAT---AQKGGR 480
            ALF+QY+I TL GPPL+EI K EEE+ER+  EV KL+NAG TIPP LK++   + +  +
Sbjct: 435 EALFNQYLIETLSGPPLIEIQKAEEEQERIVSEVQKLQNAGVTIPPGLKSSVLSSPQSAK 494

Query: 481 VIG-------------------------EGITIDHLHKLSPKNVSAWNMKRLMKIIRHVT 540
           VIG                         +GITIDHLHKL+PKNVSAWNMKRLM IIRH +
Sbjct: 495 VIGSGRLQRTPREGKSPKLSHTFSNKDDDGITIDHLHKLNPKNVSAWNMKRLMNIIRHGS 554

Query: 541 LSTLDEQIKDSITHENESTTQIKSGYEAKVAAKKIFLNVARHGSKYIYVEDLKRFMDEDK 600
           L+TLDEQI+DS T+E+ES   IK+ YEAK AA+KIF NVA+ GSK+IY+EDL RF++ED+
Sbjct: 555 LTTLDEQIQDS-TNEDESAPHIKTEYEAKAAARKIFQNVAKPGSKFIYLEDLMRFLNEDE 614

Query: 601 ASKTMGLFEEARESRKISKSSLKNWVIYASRERTALASTLDDSKTAVKKLHRMVNILISV 660
           ASKTM LFEEA E +KISKS+LKNWV+   RER ALA TL+D+KTAV KLHR VN+LI +
Sbjct: 615 ASKTMSLFEEASERKKISKSALKNWVVNVFRERRALAFTLNDTKTAVNKLHRFVNVLIGI 674

Query: 661 IILVIWLLILGIATSKFLLFATSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGNLCEI 720
           I ++IWLLILGIAT+KFLLF +SQ+V+VAFVFGNTCKTVFE+IIFLFVMHPFDVG+ CEI
Sbjct: 675 ITVIIWLLILGIATTKFLLFISSQVVVVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEI 734

Query: 721 NGVQMIVKEMNILTTIFLRYDNQKIIIPNSLLATMAIHNFYRSPDMGDAVEFCLHICTPP 771
           +GVQM+V+EMNIL+T+FLRYDNQKII PNS+LAT AI N+YRSPDMGDAVEFC+HI TP 
Sbjct: 735 DGVQMVVEEMNILSTVFLRYDNQKIIFPNSVLATKAIGNYYRSPDMGDAVEFCIHISTPA 794

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
MSL6_ARATH6.2e-23355.13Mechanosensitive ion channel protein 6 OS=Arabidopsis thaliana GN=MSL6 PE=1 SV=1[more]
MSL5_ARATH1.5e-23153.85Mechanosensitive ion channel protein 5 OS=Arabidopsis thaliana GN=MSL5 PE=2 SV=1[more]
MSL8_ARATH2.4e-22961.57Mechanosensitive ion channel protein 8 OS=Arabidopsis thaliana GN=MSL8 PE=2 SV=2[more]
MSL4_ARATH2.3e-21150.66Mechanosensitive ion channel protein 4 OS=Arabidopsis thaliana GN=MSL4 PE=3 SV=1[more]
MSL7_ARATH2.9e-20660.23Mechanosensitive ion channel protein 7 OS=Arabidopsis thaliana GN=MSL7 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KE62_CUCSA0.0e+0074.08Uncharacterized protein OS=Cucumis sativus GN=Csa_6G124170 PE=4 SV=1[more]
A0A061FGA7_THECC8.9e-27162.20Mechanosensitive channel of small conductance-like 6, putative OS=Theobroma caca... [more]
A0A059D715_EUCGR1.6e-26762.41Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_B03162 PE=4 SV=1[more]
V4T5R5_9ROSI1.1e-26057.56Uncharacterized protein OS=Citrus clementina GN=CICLE_v10018823mg PE=4 SV=1[more]
A0A0A0K619_CUCSA2.3e-25857.30Uncharacterized protein OS=Cucumis sativus GN=Csa_7G017150 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G78610.13.5e-23455.13 mechanosensitive channel of small conductance-like 6[more]
AT3G14810.18.6e-23353.85 mechanosensitive channel of small conductance-like 5[more]
AT1G53470.11.3e-21250.66 mechanosensitive channel of small conductance-like 4[more]
AT2G17000.11.6e-20760.23 Mechanosensitive ion channel family protein[more]
AT2G17010.11.2e-12262.87 Mechanosensitive ion channel family protein[more]
Match NameE-valueIdentityDescription
gi|659095000|ref|XP_008448342.1|0.0e+0074.20PREDICTED: mechanosensitive ion channel protein 6-like [Cucumis melo][more]
gi|449444568|ref|XP_004140046.1|0.0e+0074.08PREDICTED: mechanosensitive ion channel protein 6-like [Cucumis sativus][more]
gi|590611003|ref|XP_007021979.1|1.3e-27062.20Mechanosensitive channel of small conductance-like 6, putative [Theobroma cacao][more]
gi|702276066|ref|XP_010044420.1|2.3e-26762.41PREDICTED: mechanosensitive ion channel protein 6-like [Eucalyptus grandis][more]
gi|568848026|ref|XP_006477826.1|4.1e-26157.91PREDICTED: mechanosensitive ion channel protein 6 [Citrus sinensis][more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0055085transmembrane transport
Vocabulary: Cellular Component
TermDefinition
GO:0016020membrane
Vocabulary: INTERPRO
TermDefinition
IPR016688MscS-like_plants/fungi
IPR010920LSM_dom_sf
IPR006685MscS_channel
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi10G008120.1Lsi10G008120.1mRNA


Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006685Mechanosensitive ion channel MscSPFAMPF00924MS_channelcoord: 563..763
score: 1.6
IPR010920LSM domainunknownSSF50182Sm-like ribonucleoproteinscoord: 611..670
score: 1.73
IPR016688Mechanosensitive ion channel MscS-like, plants/fungiPIRPIRSF017209Memb_At2g17000coord: 5..773
score: 4.0E
NoneNo IPR availableunknownCoilCoilcoord: 378..398
scor
NoneNo IPR availableGENE3DG3DSA:2.30.30.60coord: 621..670
score: 6.1
NoneNo IPR availablePANTHERPTHR31618FAMILY NOT NAMEDcoord: 164..770
score:
NoneNo IPR availablePANTHERPTHR31618:SF1MECHANOSENSITIVE ION CHANNEL PROTEIN 6-RELATEDcoord: 164..770
score:

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None