Lsi10G007400 (gene) Bottle gourd (USVL1VR-Ls)

NameLsi10G007400
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionPectin lyase-like superfamily protein
Locationchr10 : 10218820 .. 10223908 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAAACGCAATGTCCACGCCTCCAGTTGTAGGCCTGTAGCCTTCTCCTCCTCCGATATTCATCTCCATATTACCAAGACAAAACTCTTCCAATAATATTCCCAATCGCTTTCTTCTTCTTCTTCTCCCAATTTTCATTTTCATTAATGGACGGTGACAAATCAAAATTCAGTTTCGGCCCTCCGATCAATCCCTCCTGTTCTTCCCTTCTCCTCTTCTTCATCTTCCTCGCGATTCTCACCTTCCAAATCGCCACCAGAACCCCTTTTCCTATCTGGCTTCCTTCCACTTCGTCCTCCGATTTCCAAAACCCGACCTGCTCCGCTTTCTTCCGGAGGGATGCGGCGGAGGCGGCGGTTGTGATGTCGATAACGGATTTCGGAGGAGTAGGAGACGGGAGGACATCGAACACGGAAGCGTTTCGTAAGGCGATTGAGTACATGCGGCGGTTCGGCGAGAAAGGCGGGTCTAAACTGACCGTTCCGAAGGGCAGCTGGGTCACGGGCAGCTTTAATCTCACCAGTAATTTCACGCTCTTTCTCCAACGTGGCGCTCTTATTTTGGCTTCCCAGGTCCGCTTTTTTTTAGTTTTTATTTTTACTCGATATAGAAATTGATTTAATTTAAATAAATTATAGGAGATGGAAATTAAAATTCAAATCACTATATTACATATGGTTGAACTAAAATAATATTACGGATTGAAATTGAGAATGGTTGGATCAAAATCAAATCAAATGGGTAATTTCAGCTTGTCTTTTTGTAATTTGTTTTACTTCTTGAAGGGTTTGTTCTAAAAATTCATCTGAGTAAGTCTTTCTGTGGTGTTTTTTTACTGCTGGGGAATTTTTTTTATATTATTTTTTGAAAAAAAACGAGTTTAGAAAGTACCAACTTTCTTTCTTTGTGATTCTGAGGAAGAAGAAAATGCCAAATGGGTTTTTTATTTTTATAACTTAGCAGAAAAGGAAAAATCAGAGTGATGACTCTGGCGGTCTCACCTATGTTGTTATTCTATTTTTAAACCAATAAACATGGCTAGTGGGGCCCACCCTCAACAATGCACAACACTGCTTTGTAGTGCAACACACAAAATAAGCCCAAAAATATCATTAATATAAATAATATATATGTTTACTTATTTGAAAGTAATAAAATATTTATTTATTAATTTAAATTTATTTTTAATATTTTTATAATTTTTTTAAAAATATTCATCGAAATCGATATTTTATTGATATTTTCATCTAAATTTCTATAAAATTAAGACTTCGATATTTCCATCAACATCTATATTTTAACCTTGGTTTTAGTCCCTATTAATTGAGTTTAATCAATGTTGTTTTTTGAAAAAAAAAATTAATCAATTCTAGTTAATATGTAAAATATTAGTCACTAAACTTTTAATCAATCTTATATTTTGTTCTTTAGAATTATTTTTTATCGAAATTGATTAAATAATAATAATAATTTTTATGCAAGAAAATACAATACGTAAATATGTTTTCAAAATTTATAGTGAAAATGCTAATAGATAATAATTTTTTTTTGAAAAAAAATCAATAATAAACTAACAATAGAGATTAAATTTAAGATTTTTGAAAGTACGTGAACTACTATTAGACATTTGAAATTATAGATACTAAAATTGAACAATTCTCAAAGCACAAGGACCAAAATGACATTTTAATGTAAAATAAAATAAATTACAAATTTAAGTTTTGAACATCCATACTTATTTGTTCTCTAAACCTAAAAAAATGCGTGACAAGTTCTTGAACTTCTAAAATTGTGTTTATTTGATTTTTTGAATGGCAAAAAGTGTTTAATAGATTCAAAACTTTTAATTTTATGTCAAATAGGTCTCTAAATACTCAATTTTGTATTTAATTAGTCCTTGCCCTATTCAACATTTTATAAATTTTATAGATTTACTAGACACAAAATTAAAAAATTTAGACATATCAATTTTTTTTTTTGAAAATTTAGAGTTTTATTAAACATTAGATTCAATCCATTAATTTAAACTGGGTTGAATATGTCAAATAAAAATTTGATAATTCTAGAAAATTTAAGTATCTTTAATTCATGAAGTTAATACTTGCTGAGGGTTTTTTTTTTCCTTAGGCTTAGGTTTTCTTAGTTGAAGAAGTAAATTTGTAATTAACCGAAGATGATAATAATAGAGCAGTAAAATTATGAGAGAAAAAGTTGTATGCTAATGATTTATTGGCCCTATGAATTTATCATTTAATGCATTCATTAGTGCTGAAGCTGTATTTCAAATGATGAACATGGTTCATCATTTTTAATTTCAATTTTATATCAGTGTTTGGGTTTTGCCTTTATTGTTTTACAAATTCCTTTAGGAAAATAAAATAAAAATAAAAAAAGCTTGTGCTTCCTTTCCCCCAAGGTGTGTAAATGTGAACAGAGCTTTTATGATAACTGGTTGACATTTTTTTTTCTCTTTTTTGGTGGGTATTATTTGAAATTAAATAAACTATAGTGTAACCAATAAAATAAATTATCAAATCAAATTTGGCTGTTTGCTTTATCAATCATGGTGGCATGTTAGTGTCAAAAGTTTAGTGTTATTTATTTTTTAAATATTTGTTTGAATGAATAAATAGGATCCAGATGAGTGGCCCATTATAGAGCCACTTCCTTCATATGGAAGAGGAAGAGAGAGATTGGGAGGAAGACACATCAGCCTTATTCATGGCAATGGCCTTTCCAATGTTGTCATTACTGGTACTCTCACTTTTCTTTTCTTTTCTTTTCTTTTCATTCTTATTTCTTTATTTATTTGCCTGTTTTTTTATTTTTGTTTTTGGCTCCATCTTCATTGCTAGGATTTTATTATATTTGAGGATTCTTGGTTTTTGTAAAGTAATGGAATCAAACTTTGATCATGACTAAGCTATTAGGAACCAAGATAAGATTAAAAAAAAAGATTTAGGGCCAAGAATATTATTTCTGTTTCTCAACAGTATTTGCTAGTGTTTTTATATGTGGGGAGGGATGGATTTGGGCGACAATGGTTGGGTAAGATTTTATGTTAAGTGGTCACATGCATCAGGTGTAAAAGGTTAAGAGGGAATGTGTTCAATCTATGGTGATCACCTATTTAGGATTTAATATTCTACGAATTTCCTAAATAATATTCTACGAATTTCCTTGACACTCCAATATTGTAAAGTCAAACGAGTTGTACAATAAAATTAGTCAAGGTTATGCATAAGCTGGCTGGGACACTCACAAATAAAAAAAAAAAAGGGAGAAAAGTTAGGTATTTGAATATGAAGCATAAGATGCCACCAACCTGACTAGTATAGTTCAACTAGTTAAAGCATATATATCTTTGACTAAAATATTAGATGTTTGAACTGCACATATTGTTGAACTCAAAGAAATATTAGTAAAGCCAATCCATAAAGATTAGGCATAAGAAGTTTTTACCCACCTTACATAAAAGATAAAATGTTTATCAAATGAGATCATAACTTTTGATCTACATTAGCTTTGTGTATTGAAACTAGAAATAGTGTAAACTCTTCTTCTCGTTTATCTTTGATTCGCGCTCGAGTTCGAGAATGTTCTAGATTATGAAAATCTAATGAGTTTGTGTGTTGTTTTTCAGGACAGAATGGAACTATTGATGGGCAAGGCAAGATGTGGTGGGAGCTTTGGTGGAATAGAACATTAAATCACACAAGGGGCCATCTTGTAGAGCTCATGAACTCTCACAATATTCTCATCACCAATCTCACATTCAAAAATTCCCCATTCTGGACCATCCATCCTGTCTATTGCAGGTTTCATTCTCAATCTAATGCTCTTTGTCATTTTATAGACTTGTGTTTGGTTCTTGAATTTGTTTTTGTTTTCTTTTTATAATTTCAGCAATGTTGTTATCAAAGACATGACGATCTTGGCTCCGCTCAATGCCCCGAACACAGACGGTATCGATCCAGGTATTTTGCATCTTTCTTGGATTACATATCCAATTAGGAAGTATGAGAAATAAATGAAAACAATGGTGAAGTTCATCTTCATTTTGGTGTAGATTCGAGTACCAATGTTTGTATTGAGGATTGTTATATCGAAAGTGGAGATGATTTAGTAGCAGTGAAGAGTGGATGGGATGAATATGGAATCAACCTTGCACGCCCGAGCTCGAACATCATCATAAGGCGAGTTTCTGGCACCACTCCAACCTGTTCTGGTGTCGGAATAGGAAGCGAGATGTCGGGTGGAATATCAAACATTACGATCGATGATTTAAACGTTTGGGATTCGGCAGCTGGCATTCGGATCAAGTCCGACCAAGGTAGAGGAGGATATATTGCAAATGTTAGTGTAAGTAACTTTGTAATGAACCGAGTGAAAATGGCTATAAGGTTTAGCAGGGGTTCAAATGATCATCCTAATGAACAATGGGATCCAAAGGCAGTTCCTAAAGTGAAGGGCATTTTCATTACCAATGTCATGAGCTTTAATTCGACGAAGGCTCCGGTTCTCGATGGGATCGAGGGCACGTCGTATGACGGTGTATGCATGAAAAATGTCACCATTCTCGGTCTCGCTCCCTCTGCAAAATGGCATTGTGCTTTTGTCTCAGGTTATAGCACTTCAGTCTTTCCTATGCCATGCTCTCAGTTGCAGAACACCACATTTTCATCGTTGTGCTCGTAATTTTAAGCGTATGCAGCATAGTTTTCGTTAACTATTCGGTATGACATGATTCGAAGTCGTGCTCGGAGTAATTTTGTTTTCAAAATTTGAGTTATAATACGGCGATTCATCGAGCATTGCTAGCCAGACATGAGCTTTACTAGTAGTGATCTGTGTAAGTTTTTATACTAGAAGAAGGGGTTTTATTTAACAAATCACACATGAGATTTTTTGTTGCCTTCTTGGTTTATTATTCAAATGAATCTAATTATCAAAATATCATCAACAGTTTACTAACTGACCCAACAATAGGATCTGTTGTACAAAGTGAGGGATATCTGTATTATTTTAACATTTGATACTTATATTTATTTATTTCTTTGGAAATAAGAAGAAAGTTAGGCCCCATTTGCT

mRNA sequence

CAAACGCAATGTCCACGCCTCCAGTTGTAGGCCTGTAGCCTTCTCCTCCTCCGATATTCATCTCCATATTACCAAGACAAAACTCTTCCAATAATATTCCCAATCGCTTTCTTCTTCTTCTTCTCCCAATTTTCATTTTCATTAATGGACGGTGACAAATCAAAATTCAGTTTCGGCCCTCCGATCAATCCCTCCTGTTCTTCCCTTCTCCTCTTCTTCATCTTCCTCGCGATTCTCACCTTCCAAATCGCCACCAGAACCCCTTTTCCTATCTGGCTTCCTTCCACTTCGTCCTCCGATTTCCAAAACCCGACCTGCTCCGCTTTCTTCCGGAGGGATGCGGCGGAGGCGGCGGTTGTGATGTCGATAACGGATTTCGGAGGAGTAGGAGACGGGAGGACATCGAACACGGAAGCGTTTCGTAAGGCGATTGAGTACATGCGGCGGTTCGGCGAGAAAGGCGGGTCTAAACTGACCGTTCCGAAGGGCAGCTGGGTCACGGGCAGCTTTAATCTCACCAGTAATTTCACGCTCTTTCTCCAACGTGGCGCTCTTATTTTGGCTTCCCAGGATCCAGATGAGTGGCCCATTATAGAGCCACTTCCTTCATATGGAAGAGGAAGAGAGAGATTGGGAGGAAGACACATCAGCCTTATTCATGGCAATGGCCTTTCCAATGTTGTCATTACTGGACAGAATGGAACTATTGATGGGCAAGGCAAGATGTGGTGGGAGCTTTGGTGGAATAGAACATTAAATCACACAAGGGGCCATCTTGTAGAGCTCATGAACTCTCACAATATTCTCATCACCAATCTCACATTCAAAAATTCCCCATTCTGGACCATCCATCCTGTCTATTGCAGCAATGTTGTTATCAAAGACATGACGATCTTGGCTCCGCTCAATGCCCCGAACACAGACGGTATCGATCCAGATTCGAGTACCAATGTTTGTATTGAGGATTGTTATATCGAAAGTGGAGATGATTTAGTAGCAGTGAAGAGTGGATGGGATGAATATGGAATCAACCTTGCACGCCCGAGCTCGAACATCATCATAAGGCGAGTTTCTGGCACCACTCCAACCTGTTCTGGTGTCGGAATAGGAAGCGAGATGTCGGGTGGAATATCAAACATTACGATCGATGATTTAAACGTTTGGGATTCGGCAGCTGGCATTCGGATCAAGTCCGACCAAGGTAGAGGAGGATATATTGCAAATGTTAGTGTAAGTAACTTTGTAATGAACCGAGTGAAAATGGCTATAAGGTTTAGCAGGGGTTCAAATGATCATCCTAATGAACAATGGGATCCAAAGGCAGTTCCTAAAGTGAAGGGCATTTTCATTACCAATGTCATGAGCTTTAATTCGACGAAGGCTCCGGTTCTCGATGGGATCGAGGGCACGTCGTATGACGGTGTATGCATGAAAAATGTCACCATTCTCGGTCTCGCTCCCTCTGCAAAATGGCATTGTGCTTTTGTCTCAGGTTATAGCACTTCAGTCTTTCCTATGCCATGCTCTCAGTTGCAGAACACCACATTTTCATCGTTGTGCTCGTAATTTTAAGCGTATGCAGCATAGTTTTCGTTAACTATTCGGTATGACATGATTCGAAGTCGTGCTCGGAGTAATTTTGTTTTCAAAATTTGAGTTATAATACGGCGATTCATCGAGCATTGCTAGCCAGACATGAGCTTTACTAGTAGTGATCTGTGTAAGTTTTTATACTAGAAGAAGGGGTTTTATTTAACAAATCACACATGAGATTTTTTGTTGCCTTCTTGGTTTATTATTCAAATGAATCTAATTATCAAAATATCATCAACAGTTTACTAACTGACCCAACAATAGGATCTGTTGTACAAAGTGAGGGATATCTGTATTATTTTAACATTTGATACTTATATTTATTTATTTCTTTGGAAATAAGAAGAAAGTTAGGCCCCATTTGCT

Coding sequence (CDS)

ATGGACGGTGACAAATCAAAATTCAGTTTCGGCCCTCCGATCAATCCCTCCTGTTCTTCCCTTCTCCTCTTCTTCATCTTCCTCGCGATTCTCACCTTCCAAATCGCCACCAGAACCCCTTTTCCTATCTGGCTTCCTTCCACTTCGTCCTCCGATTTCCAAAACCCGACCTGCTCCGCTTTCTTCCGGAGGGATGCGGCGGAGGCGGCGGTTGTGATGTCGATAACGGATTTCGGAGGAGTAGGAGACGGGAGGACATCGAACACGGAAGCGTTTCGTAAGGCGATTGAGTACATGCGGCGGTTCGGCGAGAAAGGCGGGTCTAAACTGACCGTTCCGAAGGGCAGCTGGGTCACGGGCAGCTTTAATCTCACCAGTAATTTCACGCTCTTTCTCCAACGTGGCGCTCTTATTTTGGCTTCCCAGGATCCAGATGAGTGGCCCATTATAGAGCCACTTCCTTCATATGGAAGAGGAAGAGAGAGATTGGGAGGAAGACACATCAGCCTTATTCATGGCAATGGCCTTTCCAATGTTGTCATTACTGGACAGAATGGAACTATTGATGGGCAAGGCAAGATGTGGTGGGAGCTTTGGTGGAATAGAACATTAAATCACACAAGGGGCCATCTTGTAGAGCTCATGAACTCTCACAATATTCTCATCACCAATCTCACATTCAAAAATTCCCCATTCTGGACCATCCATCCTGTCTATTGCAGCAATGTTGTTATCAAAGACATGACGATCTTGGCTCCGCTCAATGCCCCGAACACAGACGGTATCGATCCAGATTCGAGTACCAATGTTTGTATTGAGGATTGTTATATCGAAAGTGGAGATGATTTAGTAGCAGTGAAGAGTGGATGGGATGAATATGGAATCAACCTTGCACGCCCGAGCTCGAACATCATCATAAGGCGAGTTTCTGGCACCACTCCAACCTGTTCTGGTGTCGGAATAGGAAGCGAGATGTCGGGTGGAATATCAAACATTACGATCGATGATTTAAACGTTTGGGATTCGGCAGCTGGCATTCGGATCAAGTCCGACCAAGGTAGAGGAGGATATATTGCAAATGTTAGTGTAAGTAACTTTGTAATGAACCGAGTGAAAATGGCTATAAGGTTTAGCAGGGGTTCAAATGATCATCCTAATGAACAATGGGATCCAAAGGCAGTTCCTAAAGTGAAGGGCATTTTCATTACCAATGTCATGAGCTTTAATTCGACGAAGGCTCCGGTTCTCGATGGGATCGAGGGCACGTCGTATGACGGTGTATGCATGAAAAATGTCACCATTCTCGGTCTCGCTCCCTCTGCAAAATGGCATTGTGCTTTTGTCTCAGGTTATAGCACTTCAGTCTTTCCTATGCCATGCTCTCAGTTGCAGAACACCACATTTTCATCGTTGTGCTCGTAA

Protein sequence

MDGDKSKFSFGPPINPSCSSLLLFFIFLAILTFQIATRTPFPIWLPSTSSSDFQNPTCSAFFRRDAAEAAVVMSITDFGGVGDGRTSNTEAFRKAIEYMRRFGEKGGSKLTVPKGSWVTGSFNLTSNFTLFLQRGALILASQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNGLSNVVITGQNGTIDGQGKMWWELWWNRTLNHTRGHLVELMNSHNILITNLTFKNSPFWTIHPVYCSNVVIKDMTILAPLNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWDEYGINLARPSSNIIIRRVSGTTPTCSGVGIGSEMSGGISNITIDDLNVWDSAAGIRIKSDQGRGGYIANVSVSNFVMNRVKMAIRFSRGSNDHPNEQWDPKAVPKVKGIFITNVMSFNSTKAPVLDGIEGTSYDGVCMKNVTILGLAPSAKWHCAFVSGYSTSVFPMPCSQLQNTTFSSLCS
BLAST of Lsi10G007400 vs. Swiss-Prot
Match: PGLR_VITVI (Probable polygalacturonase OS=Vitis vinifera GN=GSVIVT00026920001 PE=1 SV=1)

HSP 1 Score: 414.1 bits (1063), Expect = 2.1e-114
Identity = 203/405 (50.12%), Postives = 280/405 (69.14%), Query Frame = 1

Query: 74  SITDFGGVGDGRTSNTEAFRKAIEYMRRFGEKGGSKLTVPKGSWVTGSFNLTSNFTLFLQ 133
           S+ DFGGVGDG+T NT+AF+ A+  + ++G +GG++L VP G W+TGSF+LTS+FTLFL 
Sbjct: 64  SLVDFGGVGDGQTLNTKAFQDAVSELSKYGSEGGAQLYVPAGKWLTGSFSLTSHFTLFLH 123

Query: 134 RGALILASQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNGLSNVVITGQNGTIDGQGK 193
           R A++LASQD  +WP+I+PLPSYGRGR+   GR+ SLI G  L++V+ITG NGTIDGQG 
Sbjct: 124 RDAVLLASQDISQWPVIKPLPSYGRGRDAAAGRYTSLIFGTNLTDVIITGDNGTIDGQGG 183

Query: 194 MWWELWWNRTLNHTRGHLVELMNSHNILITNLTFKNSPFWTIHPVYCSNVVIKDMTILAP 253
           +WW+ +    L +TR +L+ELM S +I I+NLT  NSP W +HPVY  N++I+ +TILAP
Sbjct: 184 LWWQRFHGGKLKYTRPYLIELMYSADIQISNLTLLNSPSWNVHPVYSRNILIQGITILAP 243

Query: 254 LNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWDEYGINLARPSSNIIIRRVSGTT 313
           + +PNTDGI+PDS TN  IEDCYI SGDD VAVKSGWDEYGI    P+  ++IRR++  +
Sbjct: 244 VRSPNTDGINPDSCTNTRIEDCYIVSGDDCVAVKSGWDEYGIAYGMPTKQLVIRRLTCIS 303

Query: 314 PTCSGVGIGSEMSGGISNITIDDLNVWDSAAGIRIKSDQGRGGYIANVSVSNFVMNRVKM 373
           P  + + +GSEMSGGI ++  +D+   +S +GIRIK+  GRGGY+ ++ V    M  +K 
Sbjct: 304 PYSAVIALGSEMSGGIQDVRAEDIVAINSESGIRIKTGIGRGGYVKDIYVRGMTMKTMKW 363

Query: 374 AIRFSRGSNDHPNEQWDPKAVPKVKGIFITNVMSFNSTKAPVLDGIEGTSYDGVCMKNVT 433
           A   +     H +  +DPKA P ++GI   ++++ N + A  L+GI    + G+C+ NVT
Sbjct: 364 AFWMTGNYGSHADNHYDPKAFPVIQGINYRDMVAENVSMAARLEGIPSDPFTGICISNVT 423

Query: 434 ILGLAPSAK---WHCAFVSGYSTSVFPMPCSQL--QNTTFSSLCS 474
           I  LA  AK   W C  V G S+ V P PCS L  Q    +SLC+
Sbjct: 424 I-HLAAKAKKVPWTCTDVEGISSGVTPTPCSTLPDQGPEKTSLCN 467

BLAST of Lsi10G007400 vs. Swiss-Prot
Match: PGLR3_ARATH (Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana GN=At3g15720 PE=1 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 2.1e-26
Identity = 116/422 (27.49%), Postives = 179/422 (42.42%), Query Frame = 1

Query: 73  MSITDFGGVGDGRTSNTEAFRKAIEYMRRFGEKGGSKLTVPKGSWVT-------GSFNLT 132
           + +T FG VGDG T +++AF KA E +      G  +  VP G           GS   T
Sbjct: 24  LDVTQFGAVGDGVTDDSQAFLKAWEAV--CSGTGDGQFVVPAGMTFMLQPLKFQGSCKST 83

Query: 133 SNFTLFLQRGALILASQ-----DPDEWPIIEPLPSYGRGRERLGGRHISLIHGNGLSNVV 192
             F   L  G L+  S+     D D+W +                          +  +V
Sbjct: 84  PVFVQML--GKLVAPSKGNWKGDKDQWILF-----------------------TDIEGLV 143

Query: 193 ITGQNGTIDGQGKMWWELWWNRTLNHTRGHLVELMNSHNILITNLTFKNSPFWTIHPVYC 252
           I G +G I+GQG  WWE         +R   ++  + +N+ ++ LT  +SP   IH   C
Sbjct: 144 IEG-DGEINGQGSSWWEH------KGSRPTALKFRSCNNLRLSGLTHLDSPMAHIHISEC 203

Query: 253 SNVVIKDMTILAPLNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWDEYGINLARP 312
           + V I  + I AP ++PNTDGID  +S+NV I+DC I +GDD +A+ SG           
Sbjct: 204 NYVTISSLRINAPESSPNTDGIDVGASSNVVIQDCIIATGDDCIAINSG----------- 263

Query: 313 SSNIIIRRVSGTTPTCSGVGIGSEMSGG----ISNITIDDLNVWDSAAGIRIKSDQGRGG 372
           +SNI I  +        G+ IGS    G    + N+ + + N   +  G RIK+ QG  G
Sbjct: 264 TSNIHISGID--CGPGHGISIGSLGKDGETATVENVCVQNCNFRGTMNGARIKTWQGGSG 323

Query: 373 YIANVSVSNFVMNRVKMAI---RFSRGSNDHPNEQWDPKAVPKVKGIFITNVMSFNSTKA 432
           Y   ++ +   ++ V+  I   +F  G +    +     AV   K +F   + +  S   
Sbjct: 324 YARMITFNGITLDNVENPIIIDQFYNGGDSDNAKDRKSSAVEVSKVVFSNFIGTSKSEYG 383

Query: 433 PVLDGIEGTSYDGVCMKNVTI----LGLAPSAKWHCAFVSGYSTSVFP-MPCSQLQNTTF 471
                 E      + ++++ I     G    A+  C  V G ST   P + C +L    F
Sbjct: 384 VDFRCSERVPCTEIFLRDMKIETASSGSGQVAQGQCLNVRGASTIAVPGLECLELSTDMF 398

BLAST of Lsi10G007400 vs. Swiss-Prot
Match: ADPG2_ARATH (Polygalacturonase ADPG2 OS=Arabidopsis thaliana GN=ADPG2 PE=2 SV=2)

HSP 1 Score: 116.3 bits (290), Expect = 9.0e-25
Identity = 111/431 (25.75%), Postives = 189/431 (43.85%), Query Frame = 1

Query: 18  CSSLLLFFIFLAILTFQIATRTPFPIWLPSTSSSDFQNP---------TCSAFFRRDAAE 77
           C++L+  F+  A+L F     +     +   S   F++          T    F R +  
Sbjct: 4   CTNLVTVFLLWALLMFSWCKASRISPNVYDHSYKRFKSDSLIKRREDITGLRSFVRASLR 63

Query: 78  AAVVMSITDFGGVGDGRTSNTEAFRKAIEYMRRFGEKGGSKLTVPKGS-WVTGSFNLTS- 137
               +S++DFG  GDG+T +T+AF  A  + +     G   L VPKG+ ++  S  LT  
Sbjct: 64  TPTTVSVSDFGAKGDGKTDDTQAFVNA--WKKACSSNGAVNLLVPKGNTYLLKSIQLTGP 123

Query: 138 -NFTLFLQRGALILASQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNGLSNVVITG-Q 197
            N  L +Q    + ASQ   ++  I                    I  +G++N+ + G  
Sbjct: 124 CNSILTVQIFGTLSASQKRSDYKDIS-----------------KWIMFDGVNNLSVDGGD 183

Query: 198 NGTIDGQGKMWWE--LWWNRTLNHTRGHL-VELMNSHNILITNLTFKNSPFWTIHPVYCS 257
            G +DG G+ WW+     N+    T+    +   NS ++++ NL  +N+    I    CS
Sbjct: 184 TGVVDGNGETWWQNSCKRNKAKPCTKAPTALTFYNSKSLIVKNLKVRNAQQIQISIEKCS 243

Query: 258 NVVIKDMTILAPLNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWDEYGINLARPS 317
           NV + ++ + AP ++PNTDGI   ++ N+ + +  I +GDD ++++SG           S
Sbjct: 244 NVQVSNVVVTAPADSPNTDGIHITNTQNIRVSESIIGTGDDCISIESG-----------S 303

Query: 318 SNIIIRRVSGTTPTCSGVGIGS----EMSGGISNITIDDLNVWDSAAGIRIKSDQGRGGY 377
            N+ I  +  T     G+ IGS         +S +T+D   +  +  G+RIK+ QG  G 
Sbjct: 304 QNVQINDI--TCGPGHGISIGSLGDDNSKAFVSGVTVDGAKLSGTDNGVRIKTYQGGSGT 363

Query: 378 IANVSVSNFVMNRVKMAIRFSRGSNDHPNEQWDPKAVPKVKGIFI---------TNVMSF 420
            +N+   N  M+ VK  I   +   D      +  AV +VK +            N ++F
Sbjct: 364 ASNIIFQNIQMDNVKNPIIIDQDYCDKSKCTTEKSAV-QVKNVVYRDISGTSASENAITF 401

BLAST of Lsi10G007400 vs. Swiss-Prot
Match: ADPG1_ARATH (Polygalacturonase ADPG1 OS=Arabidopsis thaliana GN=ADPG1 PE=2 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 8.4e-23
Identity = 103/390 (26.41%), Postives = 170/390 (43.59%), Query Frame = 1

Query: 70  AVVMSITDFGGVGDGRTSNTEAFRKAIEYMRRFGEKGGSKLTVPKGSWVT-------GSF 129
           A  +S+++FG  GDG+T +T+AF+KA  + +     G +   VPKG           G  
Sbjct: 65  ASTVSVSNFGAKGDGKTDDTQAFKKA--WKKACSTNGVTTFLVPKGKTYLLKSTRFRGPC 124

Query: 130 NLTSNFTLFLQRGALILAS--QDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNGLSNVV 189
               NF +     A    S  +D + W I+E +                    N LS  +
Sbjct: 125 KSLRNFQILGTLSASTKRSDYKDKNHWLILEDV--------------------NNLS--I 184

Query: 190 ITGQNGTIDGQGKMWWE--LWWNRTLNHTRGHL-VELMNSHNILITNLTFKNSPFWTIHP 249
             G  G I+G GK WW+     +++   T+    + L N  N+ + NL  KN+    I  
Sbjct: 185 DGGSTGIINGNGKTWWQNSCKIDKSKPCTKAPTALTLYNLKNLNVKNLRVKNAQQIQISI 244

Query: 250 VYCSNVVIKDMTILAPLNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWDEYGINL 309
             C+ V + ++ I AP ++PNTDGI   ++ N+ + +  I +GDD ++++ G        
Sbjct: 245 EKCNKVEVSNVEITAPGDSPNTDGIHITNTQNIRVSNSDIGTGDDCISIEDG-------- 304

Query: 310 ARPSSNIIIRRVSGTTPTCSGVGIGS----EMSGGISNITIDDLNVWDSAAGIRIKSDQG 369
              + N+ I  +  T     G+ IGS         +S I +D     +S  G+RIK+ QG
Sbjct: 305 ---TQNLQIFDL--TCGPGHGISIGSLGDDNSKAYVSGINVDGAKFSESDNGVRIKTYQG 364

Query: 370 RGGYIANVSVSNFVMNRVKMAIRFSRGSNDHPNEQWDPKAVPKVKGIFITNVMSFNSTKA 429
             G   N+   N  M  VK  I   +   D    + D ++  +VK +   N+   ++T  
Sbjct: 365 GSGTAKNIKFQNIRMENVKNPIIIDQDYCDKDKCE-DQESAVQVKNVVYKNISGTSATDV 416

Query: 430 PV-LDGIEGTSYDGVCMKNVTILGLAPSAK 443
            + L+  E     G+ ++NV I G   S K
Sbjct: 425 AITLNCSEKYPCQGIVLENVKIKGGTASCK 416

BLAST of Lsi10G007400 vs. Swiss-Prot
Match: PGLR_PERAE (Polygalacturonase OS=Persea americana PE=2 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 2.7e-21
Identity = 95/380 (25.00%), Postives = 172/380 (45.26%), Query Frame = 1

Query: 73  MSITDFGGVGDGRTSNTEAFRKAIEYMRRFGEKGGSKLTVPKGS-----WVTGSFNLTSN 132
           +S+ DFG  GDG T +T+AF KA     +     GS L VP+        +T S    S+
Sbjct: 85  ISVDDFGARGDG-TDDTKAFEKAW----KDACSSGSVLIVPENKNYLLKQITFSGPCKSD 144

Query: 133 FTLFLQRGALILASQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNGLSNVVITGQNGT 192
             + ++    I AS D  +W         G  R+R        I    +SN+ + G  GT
Sbjct: 145 LRVKIR--GTIEASSDQSDW--------VGHNRKRW-------IEFEDISNLTLEG-GGT 204

Query: 193 IDGQGKMWWELWWNRTLN---HTRGHLVELMNSHNILITNLTFKNSPFWTIHPVYCSNVV 252
            +G G+ WW+    R  +    +    +   +  N+++++L+ K+S    +    C +V+
Sbjct: 205 SNGNGETWWDSSCKRKKSLPCKSAPTALTFRSCKNLIVSDLSIKDSQKMHLSFDKCQDVI 264

Query: 253 IKDMTILAPLNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWDEYGINLARPSSNI 312
             ++ + AP ++PNTDGI    +  + + +  I +GDD ++++SG            S +
Sbjct: 265 ASNLMVTAPEHSPNTDGIHITGTQRIHVMNSVIGTGDDCISIESG------------SKM 324

Query: 313 IIRRVSGTTPTCS---GVGIGS----EMSGGISNITIDDLNVWDSAAGIRIKSDQGRGGY 372
           +I     T  TC    G+ IGS         +S + +D  N++D+  G+RIK+ QG  G 
Sbjct: 325 VI----ATNITCGPGHGISIGSLGDRNSEAHVSGVLVDGGNLFDTTNGLRIKTWQGGSGS 384

Query: 373 IANVSVSNFVMNRVKMAIRFSRGSNDHPNEQWDPKAVPKVKGIFITNVMSFNSTKAPV-L 432
             N+   N VM+ V   I   +   D  +   + ++  KV  +   N+   ++++  V  
Sbjct: 385 AKNIKFQNIVMHNVTNPIIIDQYYCDSKDPCPEQESAVKVSNVAYMNIRGTSASEVAVKF 425

Query: 433 DGIEGTSYDGVCMKNVTILG 437
           D  + +   G  + N+ ++G
Sbjct: 445 DCSKSSPCQGYIVGNINLVG 425

BLAST of Lsi10G007400 vs. TrEMBL
Match: A0A0A0LJW9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G334510 PE=3 SV=1)

HSP 1 Score: 895.6 bits (2313), Expect = 2.6e-257
Identity = 438/474 (92.41%), Postives = 453/474 (95.57%), Query Frame = 1

Query: 1   MDGDKSKFSFGPPINPSCSSLLLFFIFLAILTFQIATRTP-FPIWLPSTSSSDFQNPTCS 60
           MD DKSKF FGPPINPSCSSLLL FIFLAILTFQIATRTP FP WLP    SDFQ PTCS
Sbjct: 1   MDSDKSKFYFGPPINPSCSSLLLLFIFLAILTFQIATRTPPFPFWLPP---SDFQLPTCS 60

Query: 61  AFFRRDAAEAAVVMSITDFGGVGDGRTSNTEAFRKAIEYMRRFGEKGGSKLTVPKGSWVT 120
           AFFR DA  A VVMSITDFGGVGDG+TSNTEAFRKAI+YMRRFG+KGGSKLTVPKGSWVT
Sbjct: 61  AFFRPDAT-AGVVMSITDFGGVGDGKTSNTEAFRKAIQYMRRFGDKGGSKLTVPKGSWVT 120

Query: 121 GSFNLTSNFTLFLQRGALILASQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNGLSNV 180
           GSFNLTSNFTLFLQRGALILASQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGN LSNV
Sbjct: 121 GSFNLTSNFTLFLQRGALILASQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSNV 180

Query: 181 VITGQNGTIDGQGKMWWELWWNRTLNHTRGHLVELMNSHNILITNLTFKNSPFWTIHPVY 240
           VITG+NGTIDGQGKMWWELWWNRTLNHTRGHLVEL+NSHNILI+NLTFKNSPFWTIHPVY
Sbjct: 181 VITGENGTIDGQGKMWWELWWNRTLNHTRGHLVELINSHNILISNLTFKNSPFWTIHPVY 240

Query: 241 CSNVVIKDMTILAPLNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWDEYGINLAR 300
           CSNVVIKDMTILAPLNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWD+YGINLAR
Sbjct: 241 CSNVVIKDMTILAPLNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWDQYGINLAR 300

Query: 301 PSSNIIIRRVSGTTPTCSGVGIGSEMSGGISNITIDDLNVWDSAAGIRIKSDQGRGGYIA 360
           PSSNI+IRRVSGTTPTCSGVGIGSEMSGGISNITI+DLNVWDSAAGIRIKSDQGRGGYIA
Sbjct: 301 PSSNIVIRRVSGTTPTCSGVGIGSEMSGGISNITIEDLNVWDSAAGIRIKSDQGRGGYIA 360

Query: 361 NVSVSNFVMNRVKMAIRFSRGSNDHPNEQWDPKAVPKVKGIFITNVMSFNSTKAPVLDGI 420
           NVS++NFVMNRVKMAIRFSRGSNDHP+EQ+DPKAVPKVKGIFITN+MS NSTKAPVL GI
Sbjct: 361 NVSITNFVMNRVKMAIRFSRGSNDHPDEQFDPKAVPKVKGIFITNLMSLNSTKAPVLYGI 420

Query: 421 EGTSYDGVCMKNVTILGLAPSAKWHCAFVSGYSTSVFPMPCSQLQNTTFSSLCS 474
            GTSYDGVCMKNVTILGL PSAKWHCAFVSG+STSVFP PC QLQNTTFSSLCS
Sbjct: 421 AGTSYDGVCMKNVTILGLTPSAKWHCAFVSGFSTSVFPTPCPQLQNTTFSSLCS 470

BLAST of Lsi10G007400 vs. TrEMBL
Match: F6I0D8_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_04s0044g01420 PE=3 SV=1)

HSP 1 Score: 722.2 bits (1863), Expect = 4.0e-205
Identity = 341/461 (73.97%), Postives = 398/461 (86.33%), Query Frame = 1

Query: 16  PSCSSLLLFFIFLAILTFQIATRTPFPIWLP----STSSSDFQNPTCSAFFRRDAAEAAV 75
           PS + LLL FI L +  FQI T+T FP W+      T  S+ + P+C   FR +     V
Sbjct: 7   PSWAFLLLVFIVLVVSCFQIGTKTVFPRWVVLGGGETGISE-EAPSCFGLFR-EVPPRKV 66

Query: 76  VMSITDFGGVGDGRTSNTEAFRKAIEYMRRFGEKGGSKLTVPKGSWVTGSFNLTSNFTLF 135
           VMSI DFGGVGDG TSNTE FR+AI YM+ FG  GGS+L VP+G WVTGSFNLTSNFTLF
Sbjct: 67  VMSIRDFGGVGDGVTSNTETFRRAIRYMQSFGNIGGSQLNVPRGRWVTGSFNLTSNFTLF 126

Query: 136 LQRGALILASQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNGLSNVVITGQNGTIDGQ 195
           L+ GA+IL SQD ++WPIIEPLPSYGRGRERLGGRHISLIHG+ L+NVVITGQNGTIDGQ
Sbjct: 127 LEEGAVILGSQDLEDWPIIEPLPSYGRGRERLGGRHISLIHGDALANVVITGQNGTIDGQ 186

Query: 196 GKMWWELWWNRTLNHTRGHLVELMNSHNILITNLTFKNSPFWTIHPVYCSNVVIKDMTIL 255
           GKMWWELWWNRTL HTRGHL+E+ NSHNILI+NLTF NSPFWTIHPVYCSNVVIKD+TIL
Sbjct: 187 GKMWWELWWNRTLEHTRGHLLEIKNSHNILISNLTFMNSPFWTIHPVYCSNVVIKDVTIL 246

Query: 256 APLNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWDEYGINLARPSSNIIIRRVSG 315
           APLNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWD+YGI +ARPSSNII+RR+SG
Sbjct: 247 APLNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWDQYGIAMARPSSNIIVRRLSG 306

Query: 316 TTPTCSGVGIGSEMSGGISNITIDDLNVWDSAAGIRIKSDQGRGGYIANVSVSNFVMNRV 375
           TTPTCSGVGIGSEMSGGISN+T++DL++WDSAAG+RIK+D+GRGGY+ N++++N  M RV
Sbjct: 307 TTPTCSGVGIGSEMSGGISNVTMEDLHIWDSAAGVRIKTDKGRGGYVVNITINNIRMERV 366

Query: 376 KMAIRFSRGSNDHPNEQWDPKAVPKVKGIFITNVMSFNSTKAPVLDGIEGTSYDGVCMKN 435
           K+ IRFSRGSNDHP++ WDPKAVPK+KGIFI+NV+S NSTKAP+L+GIEG  Y+G+CMKN
Sbjct: 367 KVPIRFSRGSNDHPDDGWDPKAVPKIKGIFISNVVSLNSTKAPLLEGIEGAPYEGICMKN 426

Query: 436 VTILGLAPSAKWHCAFVSGYSTSVFPMPCSQLQNTTFSSLC 473
           VT+LGLAP+AKWHC FVSG++ +VFP+ C Q+Q+   SS C
Sbjct: 427 VTLLGLAPAAKWHCEFVSGFTDAVFPVSCPQMQSNVSSSWC 465

BLAST of Lsi10G007400 vs. TrEMBL
Match: W9R8E3_9ROSA (Putative polygalacturonase OS=Morus notabilis GN=L484_020700 PE=3 SV=1)

HSP 1 Score: 720.3 bits (1858), Expect = 1.5e-204
Identity = 344/479 (71.82%), Postives = 400/479 (83.51%), Query Frame = 1

Query: 1   MDGDKSKF-----SFGPPINPSCSSLLLFFIFLAILTFQIATRTPFPIWLPSTSSSDFQN 60
           MD ++S +     +F  P   S ++LLL    +A+++ QIATRT FP W         ++
Sbjct: 5   MDSERSIYGAIAGNFFRPPTASAATLLLLLTVVAVVSLQIATRTEFPRWTTPEVPVASED 64

Query: 61  P-TCSAFFRRDAAEAAVVMSITDFGGVGDGRTSNTEAFRKAIEYMRRFGEKGGSKLTVPK 120
           P +C  FF   A    VV+SI +FGGVGDG+TS TE FRKAI Y+RRFG+ GGS+L VP+
Sbjct: 65  PRSCGGFFGVAAQRRKVVVSIREFGGVGDGKTSETETFRKAIRYLRRFGDIGGSQLNVPR 124

Query: 121 GSWVTGSFNLTSNFTLFLQRGALILASQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGN 180
           G W+TGSFNLTSNFTLFL+ GA+IL SQDP+EWP+IEPLPSYGRGRERLGGRHISLIHG+
Sbjct: 125 GRWLTGSFNLTSNFTLFLEEGAVILGSQDPEEWPVIEPLPSYGRGRERLGGRHISLIHGD 184

Query: 181 GLSNVVITGQNGTIDGQGKMWWELWWNRTLNHTRGHLVELMNSHNILITNLTFKNSPFWT 240
           GL+NVVITGQNGTIDGQGKMWWELWWNRTLN+TRGHLVELMNSHNILI+NLTF NSPFWT
Sbjct: 185 GLTNVVITGQNGTIDGQGKMWWELWWNRTLNYTRGHLVELMNSHNILISNLTFLNSPFWT 244

Query: 241 IHPVYCSNVVIKDMTILAPLNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWDEYG 300
           IHPVYCSNVV+K+MTILAPLNAPNTDGIDPDSS+NVCIEDCYIESGDDLVAVKSGWD+YG
Sbjct: 245 IHPVYCSNVVVKNMTILAPLNAPNTDGIDPDSSSNVCIEDCYIESGDDLVAVKSGWDQYG 304

Query: 301 INLARPSSNIIIRRVSGTTPTCSGVGIGSEMSGGISNITIDDLNVWDSAAGIRIKSDQGR 360
           I +A PSSNII+R+VSGTTPTCSGVGIGSEMSGGISN+TI+D+++WDSAAG+RIKSD+GR
Sbjct: 305 IKMAWPSSNIIVRKVSGTTPTCSGVGIGSEMSGGISNVTIEDVHIWDSAAGVRIKSDKGR 364

Query: 361 GGYIANVSVSNFVMNRVKMAIRFSRGSNDHPNEQWDPKAVPKVKGIFITNVMSFNSTKAP 420
           GGYIAN+S+SN  M RVK+ IRFSRGSNDHP+E WD  AVPKVKGIFI+NV+S NSTKAP
Sbjct: 365 GGYIANISISNIKMERVKIPIRFSRGSNDHPDEGWDRNAVPKVKGIFISNVLSLNSTKAP 424

Query: 421 VLDGIEGTSYDGVCMKNVTILGLAPSAKWHCAFVSGYSTSVFPMPCSQLQNTTFSSLCS 474
            L GIEG S++ +C KN T+LGL  SA WHC  VSGY+  VFP PC +LQ  T  +L S
Sbjct: 425 ELKGIEGASFEEICFKNFTVLGLGQSAAWHCELVSGYANGVFPPPCPELQKATGFALSS 483

BLAST of Lsi10G007400 vs. TrEMBL
Match: A5BJL0_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_043959 PE=3 SV=1)

HSP 1 Score: 718.8 bits (1854), Expect = 4.4e-204
Identity = 338/454 (74.45%), Postives = 395/454 (87.00%), Query Frame = 1

Query: 16  PSCSSLLLFFIFLAILTFQIATRTPFPIWLP----STSSSDFQNPTCSAFFRRDAAEAAV 75
           PS + LLL FI L +  FQI T+T FP W+      T  S+ + P+C   FR +     V
Sbjct: 7   PSWAFLLLVFIVLVVSCFQIGTKTVFPRWVVLGGGETGISE-EAPSCFGLFR-EVPPRKV 66

Query: 76  VMSITDFGGVGDGRTSNTEAFRKAIEYMRRFGEKGGSKLTVPKGSWVTGSFNLTSNFTLF 135
           VMSI DFGGVGDG TSNTE FR+AI YM+ FG  GGS+L VP+G WVTGSFNLTSNFTLF
Sbjct: 67  VMSIRDFGGVGDGVTSNTETFRRAIRYMQSFGNIGGSQLNVPRGRWVTGSFNLTSNFTLF 126

Query: 136 LQRGALILASQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNGLSNVVITGQNGTIDGQ 195
           L+ GA+IL SQD ++WPIIEPLPSYGRGRERLGGRHISLIHG+ L+NVVITGQNGTIDGQ
Sbjct: 127 LEEGAVILGSQDLEDWPIIEPLPSYGRGRERLGGRHISLIHGDALANVVITGQNGTIDGQ 186

Query: 196 GKMWWELWWNRTLNHTRGHLVELMNSHNILITNLTFKNSPFWTIHPVYCSNVVIKDMTIL 255
           GKMWWELWWNRTL HTRGHL+E+ NSHNILI+NLTF NSPFWTIHPVYCSNVVIKD+TIL
Sbjct: 187 GKMWWELWWNRTLEHTRGHLLEIKNSHNILISNLTFMNSPFWTIHPVYCSNVVIKDVTIL 246

Query: 256 APLNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWDEYGINLARPSSNIIIRRVSG 315
           APLNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWD+YGI +ARPSSNII+RR+SG
Sbjct: 247 APLNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWDQYGIAMARPSSNIIVRRLSG 306

Query: 316 TTPTCSGVGIGSEMSGGISNITIDDLNVWDSAAGIRIKSDQGRGGYIANVSVSNFVMNRV 375
           TTPTCSGVGIGSEMSGGISN+T++DL++WDSAAG+RIK+D+GRGGY+ N++++N  M RV
Sbjct: 307 TTPTCSGVGIGSEMSGGISNVTMEDLHIWDSAAGVRIKTDKGRGGYVVNITINNIRMERV 366

Query: 376 KMAIRFSRGSNDHPNEQWDPKAVPKVKGIFITNVMSFNSTKAPVLDGIEGTSYDGVCMKN 435
           K+ IRFSRGSNDHP++ WDPKAVPK+KGIFI+NV+S NSTKAP+L+GIEG  Y+G+CMKN
Sbjct: 367 KVPIRFSRGSNDHPDDXWDPKAVPKIKGIFISNVVSLNSTKAPLLEGIEGAPYEGICMKN 426

Query: 436 VTILGLAPSAKWHCAFVSGYSTSVFPMPCSQLQN 466
           VT+LGLAP+AKWHC FVSG++ +VFP+ C Q+Q+
Sbjct: 427 VTLLGLAPAAKWHCEFVSGFTDAVFPVSCPQMQS 458

BLAST of Lsi10G007400 vs. TrEMBL
Match: M5X2D3_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa005015mg PE=3 SV=1)

HSP 1 Score: 711.4 bits (1835), Expect = 7.1e-202
Identity = 346/480 (72.08%), Postives = 393/480 (81.88%), Query Frame = 1

Query: 1   MDGDKSKFSFGPPINPSCSSLLLFFIFLAILTFQ-IATRTPFPIWLPST-------SSSD 60
           MD +K        I P+ +SLLL F  + +L++Q  +T    P W+ S        + SD
Sbjct: 1   MDAEKPSILVSS-IKPTWASLLLLFTIMLVLSYQTFSTMAGLPTWVGSIWPAAGSDTRSD 60

Query: 61  FQNPTCSAFFRRDAAEAAVVMSITDFGGVGDGRTSNTEAFRKAIEYMRRFGEKGGSKLTV 120
               TCS FF     +  VVMSI  FGGVGDG TSNTEAFR+AI +M+RFGE GG++L V
Sbjct: 61  HHPSTCSGFFG-GVPKRKVVMSIEAFGGVGDGATSNTEAFRRAILHMQRFGESGGAQLNV 120

Query: 121 PKGSWVTGSFNLTSNFTLFLQRGALILASQDPDEWPIIEPLPSYGRGRERLGGRHISLIH 180
           PKG W+TGSFNLTSNFTLFL+ GA+IL SQDP +WPIIEPLPSYGRGRERLGGRHISLIH
Sbjct: 121 PKGRWLTGSFNLTSNFTLFLEEGAVILGSQDPKQWPIIEPLPSYGRGRERLGGRHISLIH 180

Query: 181 GNGLSNVVITGQNGTIDGQGKMWWELWWNRTLNHTRGHLVELMNSHNILITNLTFKNSPF 240
           G+GL+NVVITG NGTIDGQGKMWWELWWNRTL HTRGHL+ELMNS NILI+NLTF NSPF
Sbjct: 181 GDGLTNVVITGNNGTIDGQGKMWWELWWNRTLEHTRGHLLELMNSQNILISNLTFLNSPF 240

Query: 241 WTIHPVYCSNVVIKDMTILAPLNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWDE 300
           WTIHPVYCSNVVIKDMTILAPLNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWD 
Sbjct: 241 WTIHPVYCSNVVIKDMTILAPLNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWDH 300

Query: 301 YGINLARPSSNIIIRRVSGTTPTCSGVGIGSEMSGGISNITIDDLNVWDSAAGIRIKSDQ 360
           YGI +ARPSSNII+RRV GTTPTCSGVGIGSEMSGGISN+TI+DL+VWDSAAG+RIK+D+
Sbjct: 301 YGIKMARPSSNIIVRRVHGTTPTCSGVGIGSEMSGGISNVTIEDLHVWDSAAGVRIKTDK 360

Query: 361 GRGGYIANVSVSNFVMNRVKMAIRFSRGSNDHPNEQWDPKAVPKVKGIFITNVMSFNSTK 420
           GRGGYIAN+S+SN +M RVK  I FSRG+NDHP++ WDPKAVP+VKGIFI+NV S NSTK
Sbjct: 361 GRGGYIANISISNILMERVKAPIMFSRGANDHPDKGWDPKAVPQVKGIFISNVFSLNSTK 420

Query: 421 APVLDGIEGTSYDGVCMKNVTILGLAPSAKWHCAFVSGYSTSVFPMPCSQLQNTTFSSLC 473
           AP L GIEG S++ +C KNVT+LGLAPSA WHC FVSG++  VFP  C QLQ+      C
Sbjct: 421 APELRGIEGASFERICFKNVTVLGLAPSAAWHCEFVSGFANGVFPAACPQLQDMDAFDWC 478

BLAST of Lsi10G007400 vs. TAIR10
Match: AT4G33440.1 (AT4G33440.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 681.8 bits (1758), Expect = 3.0e-196
Identity = 317/449 (70.60%), Postives = 388/449 (86.41%), Query Frame = 1

Query: 16  PSCSSLLLFFIFLAILTFQIATRTPFPIWLPSTSSSDFQNP-TCSAFFRRDAAEAAVVMS 75
           PS + LLL F  LAIL+ QI++ +  P+W+P+   S + +P TCS FF  D     +VMS
Sbjct: 17  PSWAFLLLVFTVLAILSLQISSNSFLPLWIPT---SQYDDPVTCSGFFNHDPFPNRIVMS 76

Query: 76  ITDFGGVGDGRTSNTEAFRKAIEYMRRFGEKGGSKLTVPKGSWVTGSFNLTSNFTLFLQR 135
           ITDFGGVGDG+TSNT AFR+A+ ++  F  +GG++L VP+G+W++GSFNLTSNFTLFL+R
Sbjct: 77  ITDFGGVGDGKTSNTAAFRRAVRHLEGFAAEGGAQLNVPEGTWLSGSFNLTSNFTLFLER 136

Query: 136 GALILASQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNGLSNVVITGQNGTIDGQGKM 195
           GALIL S+D DEWPIIEPLPSYGRGRER GGRHISLIHG+ L+NVVITG+NGTIDGQGKM
Sbjct: 137 GALILGSKDLDEWPIIEPLPSYGRGRERPGGRHISLIHGDNLTNVVITGENGTIDGQGKM 196

Query: 196 WWELWWNRTLNHTRGHLVELMNSHNILITNLTFKNSPFWTIHPVYCSNVVIKDMTILAPL 255
           WWELWWNRTL HTRGHL+EL NSHNILI+NLT  NSPFWTIHPVYCSNVVI++MTILAP+
Sbjct: 197 WWELWWNRTLVHTRGHLIELKNSHNILISNLTLLNSPFWTIHPVYCSNVVIRNMTILAPM 256

Query: 256 NAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWDEYGINLARPSSNIIIRRVSGTTP 315
           NAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWD+YG+ +ARPSSNI+IRR+SGTT 
Sbjct: 257 NAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWDQYGMAVARPSSNIVIRRISGTTR 316

Query: 316 TCSGVGIGSEMSGGISNITIDDLNVWDSAAGIRIKSDQGRGGYIANVSVSNFVMNRVKMA 375
           TCSGVGIGSEMSGGI NIT++D++VWDSAAG+RIK+D+GRGGYI+N++ +N ++ +VK+ 
Sbjct: 317 TCSGVGIGSEMSGGIFNITVEDIHVWDSAAGLRIKTDKGRGGYISNITFNNVLLEKVKVP 376

Query: 376 IRFSRGSNDHPNEQWDPKAVPKVKGIFITNVMSFNSTKAPVLDGIEGTSYDGVCMKNVTI 435
           IRFS GSNDH +++WDPKA+P+VKGI+I+NV+S NS KAP+L G+EGTS+  VC++NVT+
Sbjct: 377 IRFSSGSNDHSDDKWDPKALPRVKGIYISNVVSLNSRKAPMLLGVEGTSFQDVCLRNVTL 436

Query: 436 LGLAPSAKWHCAFVSGYSTSVFPMPCSQL 464
           LGL  + KW C  VSGY++ VFP+ C QL
Sbjct: 437 LGLPKTEKWKCKDVSGYASDVFPLSCPQL 462

BLAST of Lsi10G007400 vs. TAIR10
Match: AT3G62110.1 (AT3G62110.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 467.6 bits (1202), Expect = 9.0e-132
Identity = 226/399 (56.64%), Postives = 295/399 (73.93%), Query Frame = 1

Query: 73  MSITDFGGVGDGRTSNTEAFRKAIEYMRRFGEKGGSKLTVPKGSWVTGSFNLTSNFTLFL 132
           +SIT+FG VGDG T NT+AF+ A+ Y+  F +KGG+KL VP G W+TGSF+L S+ TL+L
Sbjct: 43  VSITEFGAVGDGVTLNTKAFQNALFYLNSFSDKGGAKLFVPAGQWLTGSFDLISHLTLWL 102

Query: 133 QRGALILASQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNGLSNVVITGQNGTIDGQG 192
            +GA IL S   + WP+++PLPSYGRGRE  G RH SLI+G  L++VVITG+NGTIDGQG
Sbjct: 103 DKGATILGSTSSENWPVVDPLPSYGRGRELPGRRHRSLIYGQNLTDVVITGENGTIDGQG 162

Query: 193 KMWWELWWNRTLNHTRGHLVELMNSHNILITNLTFKNSPFWTIHPVYCSNVVIKDMTILA 252
            +WW+ + N  LN+TR HLVELMNS  ++I+NLTF NSPFW IHPVYC +VV+K++TILA
Sbjct: 163 TVWWDWFRNGELNYTRPHLVELMNSTGLIISNLTFLNSPFWNIHPVYCRDVVVKNLTILA 222

Query: 253 PLNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWDEYGINLARPSSNIIIRRVSGT 312
           PL +PNTDG+DPDSSTNVCIEDCYI +GDDLV++KSGWDEYGI+ ARPSS I I R++G 
Sbjct: 223 PLESPNTDGVDPDSSTNVCIEDCYIVTGDDLVSIKSGWDEYGISYARPSSKIKINRLTGQ 282

Query: 313 TPTCSGVGIGSEMSGGISNITIDDLNVWDSAAGIRIKSDQGRGGYIANVSVSNFVMNRVK 372
           T + SG+ IGSEMSGG+S I I DL++++S  GIRIK+  GRGGY+ NV + N  ++ VK
Sbjct: 283 TTSSSGIAIGSEMSGGVSEIYIKDLHLFNSNTGIRIKTSAGRGGYVRNVHILNVKLDNVK 342

Query: 373 MAIRFSRGSNDHPNEQWDPKAVPKVKGIFITNVMSFNSTKAPVLDGIEGTSYDGVCMKNV 432
            AIRF+    +HP+E++DPKA+P ++ I   NV       A +L+GIEG  +  +C  NV
Sbjct: 343 KAIRFTGKYGEHPDEKYDPKALPAIEKITFENVNGDGIGVAGLLEGIEGDVFKNICFLNV 402

Query: 433 TILGLAPSAK--WHCAFVSGYSTSVFP-MPCSQLQNTTF 469
           T+     S K  W C+ V GYS  V P + C  L+ + F
Sbjct: 403 TLRVKKNSKKSPWECSNVRGYSQWVSPEITCDSLKESIF 441

BLAST of Lsi10G007400 vs. TAIR10
Match: AT4G23820.1 (AT4G23820.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 463.8 bits (1192), Expect = 1.3e-130
Identity = 229/432 (53.01%), Postives = 306/432 (70.83%), Query Frame = 1

Query: 47  STSSSDFQNPTCSAF----FRRDAAEAAVVMSITDFGGVGDGRTSNTEAFRKAIEYMRRF 106
           S+SS      TCS      +R D       +SITDFGGVGDGRT NT+AFR AI  ++  
Sbjct: 17  SSSSLGDSEATCSGIVPLRYRYDK------ISITDFGGVGDGRTVNTKAFRAAIYRIQHL 76

Query: 107 GEKGGSKLTVPKGSWVTGSFNLTSNFTLFLQRGALILASQDPDEWPIIEPLPSYGRGRER 166
             +GG+ L +P G ++T SFNLTS+ TL+L +GA+I A QD   WP+I+PLPSYGRGRE 
Sbjct: 77  KRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKGAVIRAVQDTWNWPLIDPLPSYGRGREL 136

Query: 167 LGGRHISLIHGNGLSNVVITGQNGTIDGQGKMWWELWWNRTLNHTRGHLVELMNSHNILI 226
            GGR++S IHG+GL +VVITGQNGTIDGQG++WW +W +RTL +TR +L+E  +S  I+I
Sbjct: 137 PGGRYMSFIHGDGLRDVVITGQNGTIDGQGEVWWNMWRSRTLKYTRPNLIEFKDSKEIII 196

Query: 227 TNLTFKNSPFWTIHPVYCSNVVIKDMTILAPLNAPNTDGIDPDSSTNVCIEDCYIESGDD 286
           +N+ F+NSPFW IHPVYCSNVVI  +TILAP ++PNTDGIDPDSS NVCIED YI +GDD
Sbjct: 197 SNVIFQNSPFWNIHPVYCSNVVIHHVTILAPQDSPNTDGIDPDSSYNVCIEDSYISTGDD 256

Query: 287 LVAVKSGWDEYGINLARPSSNIIIRRVSGTTPTCSGVGIGSEMSGGISNITIDDLNVWDS 346
           LVA+KSGWD+YGI   RPSSNI IRR++G++P  +G+ IGSE SGGI NI  + + + + 
Sbjct: 257 LVAIKSGWDQYGIAYGRPSSNITIRRITGSSP-FAGIAIGSETSGGIKNIIAEHITLSNM 316

Query: 347 AAGIRIKSDQGRGGYIANVSVSNFVMNRVKMAIRFSRGSNDHPNEQWDPKAVPKVKGIFI 406
             G+ IK++ GRGGYI N+ +S+  ++  K  I+ +  + DHP+E ++P A+P VKGI I
Sbjct: 317 GVGVNIKTNIGRGGYIKNIKISDVYVDTAKYGIKIAGDTGDHPDENYNPNALPVVKGIHI 376

Query: 407 TNVMSFNSTKAPVLDGIEGTSYDGVCMKNVTILGLAPSAK-WHCAFVSGYSTSVFPMPCS 466
            NV   N   A  + G++G+ + G+C+  + + G   S K W C+ VSG S  V P PCS
Sbjct: 377 KNVWGVNVRNAGSIQGLKGSPFTGICLSEINLHGSLNSYKTWKCSDVSGTSLKVSPWPCS 436

Query: 467 QLQNTTFSSLCS 474
           +L+ T  S+LCS
Sbjct: 437 ELRTTGGSNLCS 441

BLAST of Lsi10G007400 vs. TAIR10
Match: AT5G41870.1 (AT5G41870.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 429.9 bits (1104), Expect = 2.1e-120
Identity = 204/397 (51.39%), Postives = 287/397 (72.29%), Query Frame = 1

Query: 72  VMSITDFGGVGDGRTSNTEAFRKAIEYMRRFGEKG-GSKLTVPKGSWVTGSFNLTSNFTL 131
           ++SI+DFG VGDG+T NT+AF  AI+ +R       G+ L VP+G ++T SFNLTS+ TL
Sbjct: 44  MLSISDFGAVGDGKTLNTKAFNSAIDRIRNSNNSNEGTLLYVPRGVYLTQSFNLTSHMTL 103

Query: 132 FLQRGALILASQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNGLSNVVITGQNGTIDG 191
           +L  GA+I A QD ++WP+ +PLPSYGRGRE  G R+IS IHG+GL++VVITG+NGTIDG
Sbjct: 104 YLADGAVIKAVQDTEKWPLTDPLPSYGRGREHPGRRYISFIHGDGLNDVVITGRNGTIDG 163

Query: 192 QGKMWWELWWNRTLNHTRGHLVELMNSHNILITNLTFKNSPFWTIHPVYCSNVVIKDMTI 251
           QG+ WW +W + TL  TR  L+E  NS NIL++++  +NSPFWT+HPVYCSNVV+  +TI
Sbjct: 164 QGEPWWNMWRHGTLKFTRPGLIEFNNSTNILVSHVVLQNSPFWTLHPVYCSNVVVHHVTI 223

Query: 252 LAPLNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWDEYGINLARPSSNIIIRRVS 311
           LAP ++ NTDGIDPDSS+NVCIED YI +GDDLVAVKSGWDEYGI   RPS +I IRR++
Sbjct: 224 LAPTDSYNTDGIDPDSSSNVCIEDSYISTGDDLVAVKSGWDEYGIAYNRPSRDITIRRIT 283

Query: 312 GTTPTCSGVGIGSEMSGGISNITIDDLNVWDSAAGIRIKSDQGRGGYIANVSVSNFVMNR 371
           G++P  +G+ IGSE SGGI N+T++++ +++S  GI IK++ GRGG I  +++S   + +
Sbjct: 284 GSSP-FAGIAIGSETSGGIQNVTVENITLYNSGIGIHIKTNIGRGGSIQGITISGVYLEK 343

Query: 372 VKMAIRFSRGSNDHPNEQWDPKAVPKVKGIFITNVMSFNSTKAPVLDGIEGTSYDGVCMK 431
           V+  I+ S  + DHP+++++  A+P V+GI I NV      +A ++ G++ + +  +C  
Sbjct: 344 VRTGIKISGDTGDHPDDKFNTSALPIVRGITIKNVWGIKVERAGMVQGLKDSPFTNLCFS 403

Query: 432 NVTILGLAPSAKWHCAFVSGYSTSVFPMPCSQLQNTT 468
           NVT+ G   S  W C+ V G +  V P PC +L  TT
Sbjct: 404 NVTLTGTKRSPIWKCSDVVGAADKVNPTPCPELSATT 439

BLAST of Lsi10G007400 vs. TAIR10
Match: AT3G48950.1 (AT3G48950.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 414.1 bits (1063), Expect = 1.2e-115
Identity = 199/393 (50.64%), Postives = 273/393 (69.47%), Query Frame = 1

Query: 75  ITDFGGVGDGRTSNTEAFRKAIEYMRRFGEKGGSKLTVPKGSWVTGSFNLTSNFTLFLQR 134
           +TDFG VGDG+TSNT+AFR AI  + +    GG++L VP G W+TGSFNLTS+FTLF+QR
Sbjct: 47  LTDFGAVGDGKTSNTKAFRNAISKLSQMATDGGAQLVVPPGKWLTGSFNLTSHFTLFIQR 106

Query: 135 GALILASQDPDEWPIIEPLPSYGRGRERLG-GRHISLIHGNGLSNVVITGQNGTIDGQGK 194
           GA ILASQD  EWP+I PLPSYG+GR+  G GR  SLI G  L++VVITG NGTI+GQG+
Sbjct: 107 GATILASQDESEWPVIAPLPSYGKGRDGTGTGRFNSLISGTNLTDVVITGNNGTINGQGQ 166

Query: 195 MWWELWWNRTLNHTRGHLVELMNSHNILITNLTFKNSPFWTIHPVYCSNVVIKDMTILAP 254
            WW+ +  +    TR +L+E++ S NI I+N+T  +SP W IHPVYC++V++K +T+LAP
Sbjct: 167 YWWDKFKKKQFKITRPYLIEILFSKNIQISNITLIDSPSWNIHPVYCNSVIVKSVTVLAP 226

Query: 255 LNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWDEYGINLARPSSNIIIRRVSGTT 314
           +  PNTDGI+PDS TN  IEDCYI SGDD +AVKSGWD+YGI    P+  + IRR++  +
Sbjct: 227 VTVPNTDGINPDSCTNTLIEDCYIVSGDDCIAVKSGWDQYGIKFGMPTQQLSIRRLTCIS 286

Query: 315 PTCSGVGIGSEMSGGISNITIDDLNVWDSAAGIRIKSDQGRGGYIANVSVSNFVMNRVKM 374
           P  +GV +GSEMSGGI ++ I+D+ + ++ + IRIK+  GRG Y+ ++      M  +K 
Sbjct: 287 PKSAGVALGSEMSGGIKDVRIEDVTLTNTESAIRIKTAVGRGAYVKDIYARRITMKTMKY 346

Query: 375 AIRFSRGSNDHPNEQWDPKAVPKVKGIFITNVMSFNSTKAPVLDGIEGTSYDGVCMKNVT 434
               S     HP+E +DPKA+P++  I   ++ + N T +  LDGI+   + G+C+ NVT
Sbjct: 347 VFWMSGNYGSHPDEGFDPKALPEITNINYRDMTAENVTMSASLDGIDKDPFTGICISNVT 406

Query: 435 ILGLAPSAK---WHCAFVSGYSTSVFPMPCSQL 464
           I  LA  AK   W+C  V+G ++ V P PCS L
Sbjct: 407 I-ALAAKAKKMQWNCTDVAGVTSRVTPEPCSLL 438

BLAST of Lsi10G007400 vs. NCBI nr
Match: gi|659121935|ref|XP_008460889.1| (PREDICTED: probable polygalacturonase [Cucumis melo])

HSP 1 Score: 901.0 bits (2327), Expect = 9.0e-259
Identity = 440/474 (92.83%), Postives = 455/474 (95.99%), Query Frame = 1

Query: 1   MDGDKSKFSFGPPINPSCSSLLLFFIFLAILTFQIATRTP-FPIWLPSTSSSDFQNPTCS 60
           MD DKSKF FGPPINPSCSSLLL FIFLAILTFQIATRTP FP WLP    SDFQ PTCS
Sbjct: 42  MDSDKSKFYFGPPINPSCSSLLLLFIFLAILTFQIATRTPPFPFWLPP---SDFQLPTCS 101

Query: 61  AFFRRDAAEAAVVMSITDFGGVGDGRTSNTEAFRKAIEYMRRFGEKGGSKLTVPKGSWVT 120
           AFFR D   AAVVMSITDFGGVGDG+TSNTEAFRKAI+YMRRFG+KGGSKLTVPKGSWVT
Sbjct: 102 AFFRPDVT-AAVVMSITDFGGVGDGKTSNTEAFRKAIQYMRRFGDKGGSKLTVPKGSWVT 161

Query: 121 GSFNLTSNFTLFLQRGALILASQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNGLSNV 180
           GSFNLTSNFTLFLQRGALILASQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGN LSNV
Sbjct: 162 GSFNLTSNFTLFLQRGALILASQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSNV 221

Query: 181 VITGQNGTIDGQGKMWWELWWNRTLNHTRGHLVELMNSHNILITNLTFKNSPFWTIHPVY 240
           VITG+NGTIDGQGKMWWELWWNRTLNHTRGHLVEL+NSHNILI+NLTFKNSPFWTIHPVY
Sbjct: 222 VITGENGTIDGQGKMWWELWWNRTLNHTRGHLVELINSHNILISNLTFKNSPFWTIHPVY 281

Query: 241 CSNVVIKDMTILAPLNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWDEYGINLAR 300
           CSNVVIKDMTILAPLNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWD+YGINLAR
Sbjct: 282 CSNVVIKDMTILAPLNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWDQYGINLAR 341

Query: 301 PSSNIIIRRVSGTTPTCSGVGIGSEMSGGISNITIDDLNVWDSAAGIRIKSDQGRGGYIA 360
           PSSNI+IRRVSGTTPTCSGVGIGSEMSGGISNITI+DLNVWDSAAGIRIKSDQGRGGYIA
Sbjct: 342 PSSNIVIRRVSGTTPTCSGVGIGSEMSGGISNITIEDLNVWDSAAGIRIKSDQGRGGYIA 401

Query: 361 NVSVSNFVMNRVKMAIRFSRGSNDHPNEQWDPKAVPKVKGIFITNVMSFNSTKAPVLDGI 420
           NVS++NFVMNRVKMAIRFSRGSNDHP+EQ+DPKAVPKVKGIFITN+MS NSTKAPVLDGI
Sbjct: 402 NVSITNFVMNRVKMAIRFSRGSNDHPDEQFDPKAVPKVKGIFITNLMSLNSTKAPVLDGI 461

Query: 421 EGTSYDGVCMKNVTILGLAPSAKWHCAFVSGYSTSVFPMPCSQLQNTTFSSLCS 474
            GTSYDGVCMKNVTILGLAPSAKWHCAFVSG+STSVFPMPC QLQNTTFS LCS
Sbjct: 462 AGTSYDGVCMKNVTILGLAPSAKWHCAFVSGFSTSVFPMPCPQLQNTTFSPLCS 511

BLAST of Lsi10G007400 vs. NCBI nr
Match: gi|449459262|ref|XP_004147365.1| (PREDICTED: probable polygalacturonase [Cucumis sativus])

HSP 1 Score: 895.6 bits (2313), Expect = 3.8e-257
Identity = 438/474 (92.41%), Postives = 453/474 (95.57%), Query Frame = 1

Query: 1   MDGDKSKFSFGPPINPSCSSLLLFFIFLAILTFQIATRTP-FPIWLPSTSSSDFQNPTCS 60
           MD DKSKF FGPPINPSCSSLLL FIFLAILTFQIATRTP FP WLP    SDFQ PTCS
Sbjct: 1   MDSDKSKFYFGPPINPSCSSLLLLFIFLAILTFQIATRTPPFPFWLPP---SDFQLPTCS 60

Query: 61  AFFRRDAAEAAVVMSITDFGGVGDGRTSNTEAFRKAIEYMRRFGEKGGSKLTVPKGSWVT 120
           AFFR DA  A VVMSITDFGGVGDG+TSNTEAFRKAI+YMRRFG+KGGSKLTVPKGSWVT
Sbjct: 61  AFFRPDAT-AGVVMSITDFGGVGDGKTSNTEAFRKAIQYMRRFGDKGGSKLTVPKGSWVT 120

Query: 121 GSFNLTSNFTLFLQRGALILASQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNGLSNV 180
           GSFNLTSNFTLFLQRGALILASQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGN LSNV
Sbjct: 121 GSFNLTSNFTLFLQRGALILASQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSNV 180

Query: 181 VITGQNGTIDGQGKMWWELWWNRTLNHTRGHLVELMNSHNILITNLTFKNSPFWTIHPVY 240
           VITG+NGTIDGQGKMWWELWWNRTLNHTRGHLVEL+NSHNILI+NLTFKNSPFWTIHPVY
Sbjct: 181 VITGENGTIDGQGKMWWELWWNRTLNHTRGHLVELINSHNILISNLTFKNSPFWTIHPVY 240

Query: 241 CSNVVIKDMTILAPLNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWDEYGINLAR 300
           CSNVVIKDMTILAPLNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWD+YGINLAR
Sbjct: 241 CSNVVIKDMTILAPLNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWDQYGINLAR 300

Query: 301 PSSNIIIRRVSGTTPTCSGVGIGSEMSGGISNITIDDLNVWDSAAGIRIKSDQGRGGYIA 360
           PSSNI+IRRVSGTTPTCSGVGIGSEMSGGISNITI+DLNVWDSAAGIRIKSDQGRGGYIA
Sbjct: 301 PSSNIVIRRVSGTTPTCSGVGIGSEMSGGISNITIEDLNVWDSAAGIRIKSDQGRGGYIA 360

Query: 361 NVSVSNFVMNRVKMAIRFSRGSNDHPNEQWDPKAVPKVKGIFITNVMSFNSTKAPVLDGI 420
           NVS++NFVMNRVKMAIRFSRGSNDHP+EQ+DPKAVPKVKGIFITN+MS NSTKAPVL GI
Sbjct: 361 NVSITNFVMNRVKMAIRFSRGSNDHPDEQFDPKAVPKVKGIFITNLMSLNSTKAPVLYGI 420

Query: 421 EGTSYDGVCMKNVTILGLAPSAKWHCAFVSGYSTSVFPMPCSQLQNTTFSSLCS 474
            GTSYDGVCMKNVTILGL PSAKWHCAFVSG+STSVFP PC QLQNTTFSSLCS
Sbjct: 421 AGTSYDGVCMKNVTILGLTPSAKWHCAFVSGFSTSVFPTPCPQLQNTTFSSLCS 470

BLAST of Lsi10G007400 vs. NCBI nr
Match: gi|296088539|emb|CBI37530.3| (unnamed protein product [Vitis vinifera])

HSP 1 Score: 726.5 bits (1874), Expect = 3.0e-206
Identity = 346/478 (72.38%), Postives = 406/478 (84.94%), Query Frame = 1

Query: 1   MDGDKSKF--SFGPPINPSCSSLLLFFIFLAILTFQIATRTPFPIWLP----STSSSDFQ 60
           MD +K+ F  + G    PS + LLL FI L +  FQI T+T FP W+      T  S+ +
Sbjct: 1   MDSEKAWFGAAMGGFTRPSWAFLLLVFIVLVVSCFQIGTKTVFPRWVVLGGGETGISE-E 60

Query: 61  NPTCSAFFRRDAAEAAVVMSITDFGGVGDGRTSNTEAFRKAIEYMRRFGEKGGSKLTVPK 120
            P+C   FR +     VVMSI DFGGVGDG TSNTE FR+AI YM+ FG  GGS+L VP+
Sbjct: 61  APSCFGLFR-EVPPRKVVMSIRDFGGVGDGVTSNTETFRRAIRYMQSFGNIGGSQLNVPR 120

Query: 121 GSWVTGSFNLTSNFTLFLQRGALILASQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGN 180
           G WVTGSFNLTSNFTLFL+ GA+IL SQD ++WPIIEPLPSYGRGRERLGGRHISLIHG+
Sbjct: 121 GRWVTGSFNLTSNFTLFLEEGAVILGSQDLEDWPIIEPLPSYGRGRERLGGRHISLIHGD 180

Query: 181 GLSNVVITGQNGTIDGQGKMWWELWWNRTLNHTRGHLVELMNSHNILITNLTFKNSPFWT 240
            L+NVVITGQNGTIDGQGKMWWELWWNRTL HTRGHL+E+ NSHNILI+NLTF NSPFWT
Sbjct: 181 ALANVVITGQNGTIDGQGKMWWELWWNRTLEHTRGHLLEIKNSHNILISNLTFMNSPFWT 240

Query: 241 IHPVYCSNVVIKDMTILAPLNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWDEYG 300
           IHPVYCSNVVIKD+TILAPLNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWD+YG
Sbjct: 241 IHPVYCSNVVIKDVTILAPLNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWDQYG 300

Query: 301 INLARPSSNIIIRRVSGTTPTCSGVGIGSEMSGGISNITIDDLNVWDSAAGIRIKSDQGR 360
           I +ARPSSNII+RR+SGTTPTCSGVGIGSEMSGGISN+T++DL++WDSAAG+RIK+D+GR
Sbjct: 301 IAMARPSSNIIVRRLSGTTPTCSGVGIGSEMSGGISNVTMEDLHIWDSAAGVRIKTDKGR 360

Query: 361 GGYIANVSVSNFVMNRVKMAIRFSRGSNDHPNEQWDPKAVPKVKGIFITNVMSFNSTKAP 420
           GGY+ N++++N  M RVK+ IRFSRGSNDHP++ WDPKAVPK+KGIFI+NV+S NSTKAP
Sbjct: 361 GGYVVNITINNIRMERVKVPIRFSRGSNDHPDDGWDPKAVPKIKGIFISNVVSLNSTKAP 420

Query: 421 VLDGIEGTSYDGVCMKNVTILGLAPSAKWHCAFVSGYSTSVFPMPCSQLQNTTFSSLC 473
           +L+GIEG  Y+G+CMKNVT+LGLAP+AKWHC FVSG++ +VFP+ C Q+Q+   SS C
Sbjct: 421 LLEGIEGAPYEGICMKNVTLLGLAPAAKWHCEFVSGFTDAVFPVSCPQMQSNVSSSWC 476

BLAST of Lsi10G007400 vs. NCBI nr
Match: gi|731387744|ref|XP_002274138.2| (PREDICTED: probable polygalacturonase [Vitis vinifera])

HSP 1 Score: 726.5 bits (1874), Expect = 3.0e-206
Identity = 346/478 (72.38%), Postives = 406/478 (84.94%), Query Frame = 1

Query: 1   MDGDKSKF--SFGPPINPSCSSLLLFFIFLAILTFQIATRTPFPIWLP----STSSSDFQ 60
           MD +K+ F  + G    PS + LLL FI L +  FQI T+T FP W+      T  S+ +
Sbjct: 7   MDSEKAWFGAAMGGFTRPSWAFLLLVFIVLVVSCFQIGTKTVFPRWVVLGGGETGISE-E 66

Query: 61  NPTCSAFFRRDAAEAAVVMSITDFGGVGDGRTSNTEAFRKAIEYMRRFGEKGGSKLTVPK 120
            P+C   FR +     VVMSI DFGGVGDG TSNTE FR+AI YM+ FG  GGS+L VP+
Sbjct: 67  APSCFGLFR-EVPPRKVVMSIRDFGGVGDGVTSNTETFRRAIRYMQSFGNIGGSQLNVPR 126

Query: 121 GSWVTGSFNLTSNFTLFLQRGALILASQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGN 180
           G WVTGSFNLTSNFTLFL+ GA+IL SQD ++WPIIEPLPSYGRGRERLGGRHISLIHG+
Sbjct: 127 GRWVTGSFNLTSNFTLFLEEGAVILGSQDLEDWPIIEPLPSYGRGRERLGGRHISLIHGD 186

Query: 181 GLSNVVITGQNGTIDGQGKMWWELWWNRTLNHTRGHLVELMNSHNILITNLTFKNSPFWT 240
            L+NVVITGQNGTIDGQGKMWWELWWNRTL HTRGHL+E+ NSHNILI+NLTF NSPFWT
Sbjct: 187 ALANVVITGQNGTIDGQGKMWWELWWNRTLEHTRGHLLEIKNSHNILISNLTFMNSPFWT 246

Query: 241 IHPVYCSNVVIKDMTILAPLNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWDEYG 300
           IHPVYCSNVVIKD+TILAPLNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWD+YG
Sbjct: 247 IHPVYCSNVVIKDVTILAPLNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWDQYG 306

Query: 301 INLARPSSNIIIRRVSGTTPTCSGVGIGSEMSGGISNITIDDLNVWDSAAGIRIKSDQGR 360
           I +ARPSSNII+RR+SGTTPTCSGVGIGSEMSGGISN+T++DL++WDSAAG+RIK+D+GR
Sbjct: 307 IAMARPSSNIIVRRLSGTTPTCSGVGIGSEMSGGISNVTMEDLHIWDSAAGVRIKTDKGR 366

Query: 361 GGYIANVSVSNFVMNRVKMAIRFSRGSNDHPNEQWDPKAVPKVKGIFITNVMSFNSTKAP 420
           GGY+ N++++N  M RVK+ IRFSRGSNDHP++ WDPKAVPK+KGIFI+NV+S NSTKAP
Sbjct: 367 GGYVVNITINNIRMERVKVPIRFSRGSNDHPDDGWDPKAVPKIKGIFISNVVSLNSTKAP 426

Query: 421 VLDGIEGTSYDGVCMKNVTILGLAPSAKWHCAFVSGYSTSVFPMPCSQLQNTTFSSLC 473
           +L+GIEG  Y+G+CMKNVT+LGLAP+AKWHC FVSG++ +VFP+ C Q+Q+   SS C
Sbjct: 427 LLEGIEGAPYEGICMKNVTLLGLAPAAKWHCEFVSGFTDAVFPVSCPQMQSNVSSSWC 482

BLAST of Lsi10G007400 vs. NCBI nr
Match: gi|1009147001|ref|XP_015891176.1| (PREDICTED: probable polygalacturonase [Ziziphus jujuba])

HSP 1 Score: 724.2 bits (1868), Expect = 1.5e-205
Identity = 351/486 (72.22%), Postives = 403/486 (82.92%), Query Frame = 1

Query: 1   MDGDKSKFSF--GPPINPSCSSLLLFFIFLAILTFQIATRTPFPIWL---------PSTS 60
           MD +K  F    G  I PS + +LLF   +A+L+ Q   +   P W+         P  S
Sbjct: 1   MDSEKPIFGTIGGNFIRPSWAVMLLFLTLMAVLSIQFTPKAVIPRWVVSGWRPPPHPGGS 60

Query: 61  SSDFQNP-TCSAFFRRDAAEAA-VVMSITDFGGVGDGRTSNTEAFRKAIEYMRRFGEKGG 120
            +  ++P +CS FF     +   VVMSITDFGGVGDG+TSNTE FRKAI YM+RFGE GG
Sbjct: 61  ETGSEDPRSCSGFFSDGPGQKRRVVMSITDFGGVGDGKTSNTETFRKAIRYMQRFGELGG 120

Query: 121 SKLTVPKGSWVTGSFNLTSNFTLFLQRGALILASQDPDEWPIIEPLPSYGRGRERLGGRH 180
           S+L VP+G W+TGSFNLTSNFTL+L+ GA+IL SQDP EWPIIEPLPSYGRGRERLGGRH
Sbjct: 121 SQLNVPRGRWLTGSFNLTSNFTLYLEHGAVILGSQDPKEWPIIEPLPSYGRGRERLGGRH 180

Query: 181 ISLIHGNGLSNVVITGQNGTIDGQGKMWWELWWNRTLNHTRGHLVELMNSHNILITNLTF 240
           ISLIHG+GL NVVITG+NGT+DGQGKMWWELWWNRTL +TRGHL+E+MNS NILI+NLTF
Sbjct: 181 ISLIHGDGLVNVVITGKNGTVDGQGKMWWELWWNRTLEYTRGHLLEIMNSKNILISNLTF 240

Query: 241 KNSPFWTIHPVYCSNVVIKDMTILAPLNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVK 300
            NSPFWTIHPVY SNVVIKDMTILAPL APNTDGIDPDSS NVCIEDCYIESGDDLVAVK
Sbjct: 241 LNSPFWTIHPVYSSNVVIKDMTILAPLKAPNTDGIDPDSSINVCIEDCYIESGDDLVAVK 300

Query: 301 SGWDEYGINLARPSSNIIIRRVSGTTPTCSGVGIGSEMSGGISNITIDDLNVWDSAAGIR 360
           SGWD+YGI++ARPSSNII+R+VSGTTPTCSGVGIGSEMSGGISNITI+D++VWDSAAGIR
Sbjct: 301 SGWDQYGISMARPSSNIIVRKVSGTTPTCSGVGIGSEMSGGISNITIEDMHVWDSAAGIR 360

Query: 361 IKSDQGRGGYIANVSVSNFVMNRVKMAIRFSRGSNDHPNEQWDPKAVPKVKGIFITNVMS 420
           IKSD+GRGGYIAN+S+SN  M RVK+ I+FSRGSNDHP+E WD KAVP+VKGIFI+NV+S
Sbjct: 361 IKSDKGRGGYIANISISNIKMERVKIPIKFSRGSNDHPDEGWDAKAVPRVKGIFISNVLS 420

Query: 421 FNSTKAPVLDGIEGTSYDGVCMKNVTILGLAPSAKWHCAFVSGYSTSVFPMPCSQLQNTT 474
            NSTKAPVL GIEG S++ +C KNVT+LGLAPS  WHC FVSG+++ VFP PC QLQN  
Sbjct: 421 LNSTKAPVLQGIEGASFEEICFKNVTVLGLAPSTAWHCEFVSGFTSEVFPAPCPQLQNDD 480

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PGLR_VITVI2.1e-11450.12Probable polygalacturonase OS=Vitis vinifera GN=GSVIVT00026920001 PE=1 SV=1[more]
PGLR3_ARATH2.1e-2627.49Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana GN=At3g15720 PE=1 S... [more]
ADPG2_ARATH9.0e-2525.75Polygalacturonase ADPG2 OS=Arabidopsis thaliana GN=ADPG2 PE=2 SV=2[more]
ADPG1_ARATH8.4e-2326.41Polygalacturonase ADPG1 OS=Arabidopsis thaliana GN=ADPG1 PE=2 SV=1[more]
PGLR_PERAE2.7e-2125.00Polygalacturonase OS=Persea americana PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LJW9_CUCSA2.6e-25792.41Uncharacterized protein OS=Cucumis sativus GN=Csa_2G334510 PE=3 SV=1[more]
F6I0D8_VITVI4.0e-20573.97Putative uncharacterized protein OS=Vitis vinifera GN=VIT_04s0044g01420 PE=3 SV=... [more]
W9R8E3_9ROSA1.5e-20471.82Putative polygalacturonase OS=Morus notabilis GN=L484_020700 PE=3 SV=1[more]
A5BJL0_VITVI4.4e-20474.45Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_043959 PE=3 SV=1[more]
M5X2D3_PRUPE7.1e-20272.08Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa005015mg PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G33440.13.0e-19670.60 Pectin lyase-like superfamily protein[more]
AT3G62110.19.0e-13256.64 Pectin lyase-like superfamily protein[more]
AT4G23820.11.3e-13053.01 Pectin lyase-like superfamily protein[more]
AT5G41870.12.1e-12051.39 Pectin lyase-like superfamily protein[more]
AT3G48950.11.2e-11550.64 Pectin lyase-like superfamily protein[more]
Match NameE-valueIdentityDescription
gi|659121935|ref|XP_008460889.1|9.0e-25992.83PREDICTED: probable polygalacturonase [Cucumis melo][more]
gi|449459262|ref|XP_004147365.1|3.8e-25792.41PREDICTED: probable polygalacturonase [Cucumis sativus][more]
gi|296088539|emb|CBI37530.3|3.0e-20672.38unnamed protein product [Vitis vinifera][more]
gi|731387744|ref|XP_002274138.2|3.0e-20672.38PREDICTED: probable polygalacturonase [Vitis vinifera][more]
gi|1009147001|ref|XP_015891176.1|1.5e-20572.22PREDICTED: probable polygalacturonase [Ziziphus jujuba][more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0005975carbohydrate metabolic process
Vocabulary: Molecular Function
TermDefinition
GO:0004650polygalacturonase activity
Vocabulary: INTERPRO
TermDefinition
IPR012334Pectin_lyas_fold
IPR011050Pectin_lyase_fold/virulence
IPR006626PbH1
IPR000743Glyco_hydro_28
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071555 cell wall organization
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0005576 extracellular region
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004650 polygalacturonase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi10G007400.1Lsi10G007400.1mRNA


Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 169..448
score: 6.9
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 301..323
score: 4800.0coord: 240..266
score: 910.0coord: 329..350
score: 110.0coord: 267..299
score: 340.0coord: 397..431
score: 65
IPR011050Pectin lyase fold/virulence factorunknownSSF51126Pectin lyase-likecoord: 67..465
score: 1.12
IPR012334Pectin lyase foldGENE3DG3DSA:2.160.20.10coord: 69..450
score: 6.8E
NoneNo IPR availablePANTHERPTHR31339FAMILY NOT NAMEDcoord: 70..473
score:
NoneNo IPR availablePANTHERPTHR31339:SF4PECTIN LYASE-LIKE SUPERFAMILY PROTEINcoord: 70..473
score:

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None