Lsi10G006950 (gene) Bottle gourd (USVL1VR-Ls)

NameLsi10G006950
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionAcetyl esterase
Locationchr10 : 9747201 .. 9748217 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGGGCGTCCAGTTCTTATTTGGAAATCTCAACCTCCCAACTTCCATGGAAGCACAAACTTACTCTTCGAGTCGCTGCTCTACTCTTCAACACTTTTCTCCGATCAAATCTCACCGTCAATCGTTGGCTTCTCAATTTTTTCGACCCCAAAATCCCTCCCAATCCCAATCTCAACCACGCCGTTTCCTCTTCCGATCTCACCATCGATGCTTCTCGAGATCTCTTCCTTCGCATCTTCACCCCCACCGCTGCCGCTGCCGCCGCCGACTCACTCCCGGCACCGGTTATTTTCTACTTCCACGGCGGCGGCTTCGCCATCGGTTCCGCCGACTCCACTTCAATGGACTTGGCCGCCACTCGCTTCGCTAAAGAACTACGCGCCGTTGTAATCTCCGTCAATTACCGCCTCGCTCCTGAGTTCCGATTCCCCTGCCAATACGACGACGGATTCGATGCTTTGAAATTCATCGACGAAATGGACGACAGCGCATTGATGGGAAGGGCAGATCTAAGCCATTGCTTCATTTTGGGGGAAAGCGCCGGGGGGAATTTGGGCCACCACGTGGCGGTGAGGGCAAGCGAGTACGCGTTCAAGAGAGTGAAGGTGGTTGGATTTATAGCGTCGCAGCCGTTTTTCGGTGGAGAGGAGAGAACAGAGTCGGAGATTCGGCTCTGTAATGAGCCTCTGTTGACTCTGAAAATTACTGATTGGTTTTGGAAGGCGTTTTTGCCGGCCGGGGAAAACCGTGACCACGCGGCGGTGAATGTGAATGGACCCAATGGAAGGGATATATTGGGGGTGAGGAACTTTCCGGCGACGGTGATTTTCGCCGGAGAATTGGATCTTCTGATTGATCGGCATAAGAGTTATTACGAGTGGTTAATGAAAATGGAAAAGGATGTGAAGTTGGTGGTGTTCTCGAACGCCGTCCATGGATTCTCTGTCTTTGCGGATCTGCCGGAATATTCTTTGATGGTTAAAGAGATAAAGGATTTCATTGCAAAGCTGAGATAG

mRNA sequence

ATGTGGGCGTCCAGTTCTTATTTGGAAATCTCAACCTCCCAACTTCCATGGAAGCACAAACTTACTCTTCGAGTCGCTGCTCTACTCTTCAACACTTTTCTCCGATCAAATCTCACCGTCAATCGTTGGCTTCTCAATTTTTTCGACCCCAAAATCCCTCCCAATCCCAATCTCAACCACGCCGTTTCCTCTTCCGATCTCACCATCGATGCTTCTCGAGATCTCTTCCTTCGCATCTTCACCCCCACCGCTGCCGCTGCCGCCGCCGACTCACTCCCGGCACCGGTTATTTTCTACTTCCACGGCGGCGGCTTCGCCATCGGTTCCGCCGACTCCACTTCAATGGACTTGGCCGCCACTCGCTTCGCTAAAGAACTACGCGCCGTTGTAATCTCCGTCAATTACCGCCTCGCTCCTGAGTTCCGATTCCCCTGCCAATACGACGACGGATTCGATGCTTTGAAATTCATCGACGAAATGGACGACAGCGCATTGATGGGAAGGGCAGATCTAAGCCATTGCTTCATTTTGGGGGAAAGCGCCGGGGGGAATTTGGGCCACCACGTGGCGGTGAGGGCAAGCGAGTACGCGTTCAAGAGAGTGAAGGTGGTTGGATTTATAGCGTCGCAGCCGTTTTTCGGTGGAGAGGAGAGAACAGAGTCGGAGATTCGGCTCTGTAATGAGCCTCTGTTGACTCTGAAAATTACTGATTGGTTTTGGAAGGCGTTTTTGCCGGCCGGGGAAAACCGTGACCACGCGGCGGTGAATGTGAATGGACCCAATGGAAGGGATATATTGGGGGTGAGGAACTTTCCGGCGACGGTGATTTTCGCCGGAGAATTGGATCTTCTGATTGATCGGCATAAGAGTTATTACGAGTGGTTAATGAAAATGGAAAAGGATGTGAAGTTGGTGGTGTTCTCGAACGCCGTCCATGGATTCTCTGTCTTTGCGGATCTGCCGGAATATTCTTTGATGGTTAAAGAGATAAAGGATTTCATTGCAAAGCTGAGATAG

Coding sequence (CDS)

ATGTGGGCGTCCAGTTCTTATTTGGAAATCTCAACCTCCCAACTTCCATGGAAGCACAAACTTACTCTTCGAGTCGCTGCTCTACTCTTCAACACTTTTCTCCGATCAAATCTCACCGTCAATCGTTGGCTTCTCAATTTTTTCGACCCCAAAATCCCTCCCAATCCCAATCTCAACCACGCCGTTTCCTCTTCCGATCTCACCATCGATGCTTCTCGAGATCTCTTCCTTCGCATCTTCACCCCCACCGCTGCCGCTGCCGCCGCCGACTCACTCCCGGCACCGGTTATTTTCTACTTCCACGGCGGCGGCTTCGCCATCGGTTCCGCCGACTCCACTTCAATGGACTTGGCCGCCACTCGCTTCGCTAAAGAACTACGCGCCGTTGTAATCTCCGTCAATTACCGCCTCGCTCCTGAGTTCCGATTCCCCTGCCAATACGACGACGGATTCGATGCTTTGAAATTCATCGACGAAATGGACGACAGCGCATTGATGGGAAGGGCAGATCTAAGCCATTGCTTCATTTTGGGGGAAAGCGCCGGGGGGAATTTGGGCCACCACGTGGCGGTGAGGGCAAGCGAGTACGCGTTCAAGAGAGTGAAGGTGGTTGGATTTATAGCGTCGCAGCCGTTTTTCGGTGGAGAGGAGAGAACAGAGTCGGAGATTCGGCTCTGTAATGAGCCTCTGTTGACTCTGAAAATTACTGATTGGTTTTGGAAGGCGTTTTTGCCGGCCGGGGAAAACCGTGACCACGCGGCGGTGAATGTGAATGGACCCAATGGAAGGGATATATTGGGGGTGAGGAACTTTCCGGCGACGGTGATTTTCGCCGGAGAATTGGATCTTCTGATTGATCGGCATAAGAGTTATTACGAGTGGTTAATGAAAATGGAAAAGGATGTGAAGTTGGTGGTGTTCTCGAACGCCGTCCATGGATTCTCTGTCTTTGCGGATCTGCCGGAATATTCTTTGATGGTTAAAGAGATAAAGGATTTCATTGCAAAGCTGAGATAG

Protein sequence

MWASSSYLEISTSQLPWKHKLTLRVAALLFNTFLRSNLTVNRWLLNFFDPKIPPNPNLNHAVSSSDLTIDASRDLFLRIFTPTAAAAAADSLPAPVIFYFHGGGFAIGSADSTSMDLAATRFAKELRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDSALMGRADLSHCFILGESAGGNLGHHVAVRASEYAFKRVKVVGFIASQPFFGGEERTESEIRLCNEPLLTLKITDWFWKAFLPAGENRDHAAVNVNGPNGRDILGVRNFPATVIFAGELDLLIDRHKSYYEWLMKMEKDVKLVVFSNAVHGFSVFADLPEYSLMVKEIKDFIAKLR
BLAST of Lsi10G006950 vs. Swiss-Prot
Match: CXE18_ARATH (Probable carboxylesterase 18 OS=Arabidopsis thaliana GN=CXE18 PE=1 SV=1)

HSP 1 Score: 290.8 bits (743), Expect = 1.9e-77
Identity = 153/323 (47.37%), Postives = 203/323 (62.85%), Query Frame = 1

Query: 15  LPWKHKLTLRVAALLFNTFLRSNLTVNRWLLNFFDPKIPPNPNLNHAVSSSDLTIDASRD 74
           LP K ++ L V + + +   R + T+NR  L  FD + PPNP   + VS+SD  +D SRD
Sbjct: 13  LPLKTRIALTVISTMTDNAQRPDGTINRRFLRLFDFRAPPNPKPVNIVSTSDFVVDQSRD 72

Query: 75  LFLRIFTPTAAAAAADSLPAPVIFYFHGGGFAIGSADSTSMDLAATRFAKELRAVVISVN 134
           L+ R++TP  +    D +P  V+ +FHGGGFA  S ++   D    RFA++L A VISVN
Sbjct: 73  LWFRLYTPHVSG---DKIP--VVVFFHGGGFAFLSPNAYPYDNVCRRFARKLPAYVISVN 132

Query: 135 YRLAPEFRFPCQYDDGFDALKFIDEMDDSALMGRADLSHCFILGESAGGNLGHHVAV--- 194
           YRLAPE R+P QYDDGFDALK+I+E   S L   ADLS CF  G+SAGGN+ H+VA+   
Sbjct: 133 YRLAPEHRYPAQYDDGFDALKYIEENHGSILPANADLSRCFFAGDSAGGNIAHNVAIRIC 192

Query: 195 RASEYAFKRVKVVGFIASQPFFGGEERTESEIRLCNEPLLTLKITDWFWKAFLPAGENRD 254
           R    +F  VK++G I+ QPFFGGEERTE+E +L   PL++   TDW WKA    G NRD
Sbjct: 193 REPRSSFTAVKLIGLISIQPFFGGEERTEAEKQLVGAPLVSPDRTDWCWKAM---GLNRD 252

Query: 255 HAAVNVNGPNGRDILGVRNFPATVIFAGELDLLIDRHKSYYEWLMKMEKDVKLVVFSNAV 314
           H AVNV GPN  DI G+ ++P T++     D L D  +SYYEWL    K   L+ + N  
Sbjct: 253 HEAVNVGGPNAVDISGL-DYPETMVVVAGFDPLKDWQRSYYEWLKLCGKKATLIEYPNMF 312

Query: 315 HGFSVFADLPEYSLMVKEIKDFI 335
           H F +F +LPE   ++  IKDF+
Sbjct: 313 HAFYIFPELPEAGQLIMRIKDFV 326

BLAST of Lsi10G006950 vs. Swiss-Prot
Match: GID1C_ARATH (Gibberellin receptor GID1C OS=Arabidopsis thaliana GN=GID1C PE=1 SV=1)

HSP 1 Score: 212.6 bits (540), Expect = 6.6e-54
Identity = 124/317 (39.12%), Postives = 175/317 (55.21%), Query Frame = 1

Query: 28  LLFNTFLRSNLTVNRWLLNFFDPKIPPNPNLNHAVSSSDLTIDASRDLFLRIFTPTAAAA 87
           L +N   R + T NR L  F D K+P N N  + V S D+ ID   +L  R++ P  A  
Sbjct: 29  LAYNLLRRPDGTFNRHLAEFLDRKVPANANPVNGVFSFDVIIDRQTNLLSRVYRPADAGT 88

Query: 88  A---------ADSLPAPVIFYFHGGGFAIGSADSTSMDLAATRFAKELRAVVISVNYRLA 147
           +          D    PVI +FHGG FA  SA+S   D    R      AVV+SVNYR A
Sbjct: 89  SPSITDLQNPVDGEIVPVIVFFHGGSFAHSSANSAIYDTLCRRLVGLCGAVVVSVNYRRA 148

Query: 148 PEFRFPCQYDDGFDALKFIDEMDDSALMGRADLS-HCFILGESAGGNLGHHVAVRASEYA 207
           PE R+PC YDDG+  LK+++    S L  + D     F+ G+S+GGN+ H+VAVRA E  
Sbjct: 149 PENRYPCAYDDGWAVLKWVNS--SSWLRSKKDSKVRIFLAGDSSGGNIVHNVAVRAVE-- 208

Query: 208 FKRVKVVGFIASQPFFGGEERTESEIRLCNEPLLTLKITDWFWKAFLPAGENRDHAAVNV 267
             R+ V+G I   P FGG ERTESE RL  +  +T++  DW+W+AFLP GE+R+H A + 
Sbjct: 209 -SRIDVLGNILLNPMFGGTERTESEKRLDGKYFVTVRDRDWYWRAFLPEGEDREHPACSP 268

Query: 268 NGPNGRDILGVRNFPATVIFAGELDLLIDRHKSYYEWLMKMEKDVKLVVFSNAVHGFSVF 327
            GP  + + G+ +FP +++    LDL+ D    Y E L K  ++VKL+    A  GF + 
Sbjct: 269 FGPRSKSLEGL-SFPKSLVVVAGLDLIQDWQLKYAEGLKKAGQEVKLLYLEQATIGFYLL 328

Query: 328 ADLPEYSLMVKEIKDFI 335
            +   +  ++ EI  F+
Sbjct: 329 PNNNHFHTVMDEIAAFV 339

BLAST of Lsi10G006950 vs. Swiss-Prot
Match: GID1_ORYSJ (Gibberellin receptor GID1 OS=Oryza sativa subsp. japonica GN=GID1 PE=1 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 1.1e-53
Identity = 131/325 (40.31%), Postives = 173/325 (53.23%), Query Frame = 1

Query: 28  LLFNTFLRSNLTVNRWLLNFFDPKIPPNPNLNHAVSSSDLTIDASRDLFLRIFTPTAAAA 87
           L +N   R++ T  R L  + D ++P N      VSS D  ID S  L +RI+   A   
Sbjct: 29  LSYNILRRADGTFERDLGEYLDRRVPANARPLEGVSSFDHIIDQSVGLEVRIYRAAAEGD 88

Query: 88  AADSL------------------PAPVIFYFHGGGFAIGSADSTSMDLAATRFAKELRAV 147
           A +                    P PVI +FHGG F   SA ST  D    RF K  + V
Sbjct: 89  AEEGAAAVTRPILEFLTDAPAAEPFPVIIFFHGGSFVHSSASSTIYDSLCRRFVKLSKGV 148

Query: 148 VISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDSALMGRADLSHCFILGESAGGNLGHHV 207
           V+SVNYR APE R+PC YDDG+ ALK++         G A  +  F+ G+S+GGN+ HHV
Sbjct: 149 VVSVNYRRAPEHRYPCAYDDGWTALKWVMSQPFMRSGGDAQ-ARVFLSGDSSGGNIAHHV 208

Query: 208 AVRASEYAFKRVKVVGFIASQPFFGGEERTESEIRLCNEPLLTLKITDWFWKAFLPAGEN 267
           AVRA++   + VKV G I     FGG ERTESE RL  +  +TL+  DW+WKA+LP   +
Sbjct: 209 AVRAAD---EGVKVCGNILLNAMFGGTERTESERRLDGKYFVTLQDRDWYWKAYLPEDAD 268

Query: 268 RDHAAVNVNGPNGRDILGVRNFPATVIFAGELDLLIDRHKSYYEWLMKMEKDVKLVVFSN 327
           RDH A N  GPNGR  LG   F  ++I    LDL  DR  +Y + L +    VK+V   N
Sbjct: 269 RDHPACNPFGPNGRR-LGGLPFAKSLIIVSGLDLTCDRQLAYADALREDGHHVKVVQCEN 328

Query: 328 AVHGFSVFADLPEYSLMVKEIKDFI 335
           A  GF +  +   Y  +++EI DF+
Sbjct: 329 ATVGFYLLPNTVHYHEVMEEISDFL 348

BLAST of Lsi10G006950 vs. Swiss-Prot
Match: GID1A_ARATH (Gibberellin receptor GID1A OS=Arabidopsis thaliana GN=GID1A PE=1 SV=1)

HSP 1 Score: 202.6 bits (514), Expect = 6.8e-51
Identity = 120/317 (37.85%), Postives = 170/317 (53.63%), Query Frame = 1

Query: 30  FNTFLRSNLTVNRWLLNFFDPKIPPNPNLNHAVSSSDLTIDASRDLFLRIFTPTAA---- 89
           +N   R + T NR L  + D K+  N N    V S D+ ID   +L  R++ P  A    
Sbjct: 31  YNILRRPDGTFNRHLAEYLDRKVTANANPVDGVFSFDVLIDRRINLLSRVYRPAYADQEQ 90

Query: 90  -------AAAADSLPAPVIFYFHGGGFAIGSADSTSMDLAATRFAKELRAVVISVNYRLA 149
                      D    PVI +FHGG FA  SA+S   D    R     + VV+SVNYR A
Sbjct: 91  PPSILDLEKPVDGDIVPVILFFHGGSFAHSSANSAIYDTLCRRLVGLCKCVVVSVNYRRA 150

Query: 150 PEFRFPCQYDDGFDALKFIDEMDDSALMGRADLS-HCFILGESAGGNLGHHVAVRASEYA 209
           PE  +PC YDDG+ AL +++    S L  + D   H F+ G+S+GGN+ H+VA+RA E  
Sbjct: 151 PENPYPCAYDDGWIALNWVNSR--SWLKSKKDSKVHIFLAGDSSGGNIAHNVALRAGESG 210

Query: 210 FKRVKVVGFIASQPFFGGEERTESEIRLCNEPLLTLKITDWFWKAFLPAGENRDHAAVNV 269
              + V+G I   P FGG ERTESE  L  +  +T++  DW+WKAFLP GE+R+H A N 
Sbjct: 211 ---IDVLGNILLNPMFGGNERTESEKSLDGKYFVTVRDRDWYWKAFLPEGEDREHPACNP 270

Query: 270 NGPNGRDILGVRNFPATVIFAGELDLLIDRHKSYYEWLMKMEKDVKLVVFSNAVHGFSVF 329
             P G+ + GV +FP +++    LDL+ D   +Y E L K  ++VKL+    A  GF + 
Sbjct: 271 FSPRGKSLEGV-SFPKSLVVVAGLDLIRDWQLAYAEGLKKAGQEVKLMHLEKATVGFYLL 330

Query: 330 ADLPEYSLMVKEIKDFI 335
            +   +  ++ EI  F+
Sbjct: 331 PNNNHFHNVMDEISAFV 341

BLAST of Lsi10G006950 vs. Swiss-Prot
Match: GID1B_ARATH (Gibberellin receptor GID1B OS=Arabidopsis thaliana GN=GID1B PE=1 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 4.6e-47
Identity = 110/319 (34.48%), Postives = 171/319 (53.61%), Query Frame = 1

Query: 28  LLFNTFLRSNLTVNRWLLNFFDPKIPPNPNLNHAVSSSDLTIDASRDLFLRIFTPTA--- 87
           L +    R + + NR L  F D K+P N      V S D  +D++ +L  RI+ P +   
Sbjct: 29  LAYKVLRRPDGSFNRDLAEFLDRKVPANSFPLDGVFSFD-HVDSTTNLLTRIYQPASLLH 88

Query: 88  ---------AAAAADSLPAPVIFYFHGGGFAIGSADSTSMDLAATRFAKELRAVVISVNY 147
                        + +   PV+ +FHGG F   SA+S   D    R       VV+SV+Y
Sbjct: 89  QTRHGTLELTKPLSTTEIVPVLIFFHGGSFTHSSANSAIYDTFCRRLVTICGVVVVSVDY 148

Query: 148 RLAPEFRFPCQYDDGFDALKFIDEMDDSALMGRADLSHCFILGESAGGNLGHHVAVRASE 207
           R +PE R+PC YDDG++AL ++         G+    + ++ G+S+GGN+ H+VAVRA+ 
Sbjct: 149 RRSPEHRYPCAYDDGWNALNWVKSRV-WLQSGKDSNVYVYLAGDSSGGNIAHNVAVRATN 208

Query: 208 YAFKRVKVVGFIASQPFFGGEERTESEIRLCNEPLLTLKITDWFWKAFLPAGENRDHAAV 267
              + VKV+G I   P FGG+ERT+SE  L  +  +T++  DW+W+A+LP GE+RDH A 
Sbjct: 209 ---EGVKVLGNILLHPMFGGQERTQSEKTLDGKYFVTIQDRDWYWRAYLPEGEDRDHPAC 268

Query: 268 NVNGPNGRDILGVRNFPATVIFAGELDLLIDRHKSYYEWLMKMEKDVKLVVFSNAVHGFS 327
           N  GP G+ + GV NFP +++    LDL+ D   +Y + L K   +V L+    A  GF 
Sbjct: 269 NPFGPRGQSLKGV-NFPKSLVVVAGLDLVQDWQLAYVDGLKKTGLEVNLLYLKQATIGFY 328

Query: 328 VFADLPEYSLMVKEIKDFI 335
              +   +  +++E+  F+
Sbjct: 329 FLPNNDHFHCLMEELNKFV 341

BLAST of Lsi10G006950 vs. TrEMBL
Match: A0A0A0KXS8_CUCSA (Acetyl esterase OS=Cucumis sativus GN=Csa_5G647390 PE=4 SV=1)

HSP 1 Score: 530.0 bits (1364), Expect = 2.1e-147
Identity = 263/338 (77.81%), Postives = 289/338 (85.50%), Query Frame = 1

Query: 1   MWASSSYLEISTSQLPWKHKLTLRVAALLFNTFLRSNLTVNRWLLNFFDPKIPPNPNLNH 60
           M +SS   E+ST QLPWKHK+TLR A LLFNT LRS+ TVNR LL F DPKIPPNPN  H
Sbjct: 1   MRSSSVCYELSTPQLPWKHKVTLRFATLLFNTSLRSDFTVNRRLLTFLDPKIPPNPNSAH 60

Query: 61  AVSSSDLTIDASRDLFLRIFTPTAAAAAADSLPA-PVIFYFHGGGFAIGSADSTSMDLAA 120
           +VSSSDLTID SRDLFLRIFTP   AA  +SLP  P+IFYFHGGGFA GSAD+TS D+AA
Sbjct: 61  SVSSSDLTIDTSRDLFLRIFTPNPTAALDESLPLLPIIFYFHGGGFAFGSADATSTDMAA 120

Query: 121 TRFAKELRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDSALMGRADLSHCFILGE 180
             FA++LRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDD +L+ R DLS CFILGE
Sbjct: 121 RGFAEKLRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDSLLERVDLSRCFILGE 180

Query: 181 SAGGNLGHHVAVRASEYAFKRVKVVGFIASQPFFGGEERTESEIRLCNEPLLTLKITDWF 240
           SAGGNLGHHVAVRASEY FKRVK++GFIASQPFFGG+ERTESE RLC +  LTL +TDWF
Sbjct: 181 SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLYMTDWF 240

Query: 241 WKAFLPAGENRDHAAVNVNGPNGRDILGVRNFPATVIFAGELDLLIDRHKSYYEWLMKME 300
           W+AFLPAGE+RDHAA NVNGPNGRDI G+ NFPATVIFAG LDLL+DR KSYYE L +M 
Sbjct: 241 WRAFLPAGEDRDHAAANVNGPNGRDISGLENFPATVIFAGGLDLLMDRQKSYYERLKRMG 300

Query: 301 KDVKLVVFSNAVHGFSVFADLPEYSLMVKEIKDFIAKL 338
           KDVKLVVFSNA HGF  F DLPEYSLM++E+ DFIAKL
Sbjct: 301 KDVKLVVFSNAFHGFFGFPDLPEYSLMIEEMSDFIAKL 338

BLAST of Lsi10G006950 vs. TrEMBL
Match: A0A0A0KUP3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G647370 PE=4 SV=1)

HSP 1 Score: 432.6 bits (1111), Expect = 4.5e-118
Identity = 212/335 (63.28%), Postives = 254/335 (75.82%), Query Frame = 1

Query: 1   MWASSSYLEISTSQLPWKHKLTLRVAALLFNTFLRSNLTVNRWLLNFFDPKIPPNPNLNH 60
           M +S+S  EIS+S+LPWKH+L LR+ + + +   RS+ TVNRW     D KIPP+     
Sbjct: 1   MSSSTSASEISSSKLPWKHRLLLRIGSTITDACCRSDFTVNRWFTGILDWKIPPSTKPID 60

Query: 61  AVSSSDLTIDASRDLFLRIFTPTAAAAAADSLPAPVIFYFHGGGFAIGSADSTSMDLAAT 120
            VSS DLTID SR+L++RIF P      +D    P+IFYFHGGGFA   ADS     +A 
Sbjct: 61  GVSSFDLTIDTSRNLWVRIFNPVIDGEDSDIQSLPLIFYFHGGGFAFSYADSALSHTSAH 120

Query: 121 RFAKELRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDSALMGRADLSHCFILGES 180
           RFAK+L AVVISVNYRLAPEFR+PCQYDDGFDALKFIDE+ +  L  +ADL+ CFILGES
Sbjct: 121 RFAKQLPAVVISVNYRLAPEFRYPCQYDDGFDALKFIDEVGEEILPAKADLTRCFILGES 180

Query: 181 AGGNLGHHVAVRASEYAFKRVKVVGFIASQPFFGGEERTESEIRLCNEPLLTLKITDWFW 240
           AGGNLGHHVAVRASEY  K+VK+VGFIASQPFFGGEERTESEIRL N+  L+L+++DWFW
Sbjct: 181 AGGNLGHHVAVRASEYTLKKVKMVGFIASQPFFGGEERTESEIRLSNQRPLSLRLSDWFW 240

Query: 241 KAFLPAGENRDHAAVNVNGPNGRDILGVRNFPATVIFAGELDLLIDRHKSYYEWLMKMEK 300
           KAFLP GE+RDH A NV GP GRD+  V  FPAT++  GELDLL D  + YYE L +M K
Sbjct: 241 KAFLPEGEDRDHGAANVFGPKGRDVTEVMKFPATLVMVGELDLLQDGQRRYYEGLKRMGK 300

Query: 301 DVKLVVFSNAVHGFSVFADLPEYSLMVKEIKDFIA 336
           +VK+V F NA+HGF  F DLP+YS M+KE+KDFIA
Sbjct: 301 EVKMVEFENAIHGFFAFWDLPQYSSMMKEMKDFIA 335

BLAST of Lsi10G006950 vs. TrEMBL
Match: A0A067KH58_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_14820 PE=4 SV=1)

HSP 1 Score: 348.6 bits (893), Expect = 8.6e-93
Identity = 171/323 (52.94%), Postives = 222/323 (68.73%), Query Frame = 1

Query: 14  QLPWKHKLTLRVAALLFNTFLRSNLTVNRWLLNFFDPKIPPNPNLNHAVSSSDLTIDASR 73
           QLPWK  L + V +   +   RSN T+NR LL  FD K  P+    + V ++D+TID SR
Sbjct: 10  QLPWKANLFISVMSFGVDIVRRSNGTINRPLLKIFDVKKSPSKKPINGVKTTDITIDKSR 69

Query: 74  DLFLRIFTPTAAAAAADSLPAPVIFYFHGGGFAIGSADSTSMDLAATRFAKELRAVVISV 133
           DL+ R++TPT    + DS   PVIFYFHGGGF++ +A+S  MD    R A+EL A +ISV
Sbjct: 70  DLWFRLYTPTTNTIS-DSAGLPVIFYFHGGGFSLLAANSRPMDDFCYRLARELSAFIISV 129

Query: 134 NYRLAPEFRFPCQYDDGFDALKFIDEMDDSALMGRADLSHCFILGESAGGNLGHHVAVRA 193
           NYRLAPE RFP QYDDGFDALK+ID  +       A+L+HCF+ G+SAGGNL HHV ++A
Sbjct: 130 NYRLAPEHRFPSQYDDGFDALKYIDSTEIEGFSSYANLNHCFVAGDSAGGNLAHHVIIKA 189

Query: 194 SEYAFKRVKVVGFIASQPFFGGEERTESEIRLCNEPLLTLKITDWFWKAFLPAGENRDHA 253
           +EY F  +K++G I+ QPF GGEERTESE+RL N P +T++ TDW W+AFLP G NRDH 
Sbjct: 190 NEYEFSNIKLIGNISFQPFLGGEERTESELRLTNAPFITIERTDWMWRAFLPGGSNRDHP 249

Query: 254 AVNVNGPNGRDILGVRNFPATVIFAGELDLLIDRHKSYYEWLMKMEKDVKLVVFSNAVHG 313
           AVN+ GPNG DI G++ FPAT++F G  D L D  K YYE L K  K+  LV + N  H 
Sbjct: 250 AVNIFGPNGVDISGMK-FPATIVFVGGYDPLQDWQKRYYEGLKKSGKEAYLVEYPNMPHS 309

Query: 314 FSVFADLPEYSLMVKEIKDFIAK 337
           F ++ +LPE  L ++E+KDF+ K
Sbjct: 310 FYIYNELPESGLSIEEVKDFMQK 330

BLAST of Lsi10G006950 vs. TrEMBL
Match: A0A067K634_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_14822 PE=4 SV=1)

HSP 1 Score: 342.0 bits (876), Expect = 8.0e-91
Identity = 168/323 (52.01%), Postives = 220/323 (68.11%), Query Frame = 1

Query: 14  QLPWKHKLTLRVAALLFNTFLRSNLTVNRWLLNFFDPKIPPNPNLNHAVSSSDLTIDASR 73
           QLPWK  L + V +   +   RSN T+NR LL  FD K  P+    + V ++D+TID SR
Sbjct: 10  QLPWKANLFISVMSFGVDIVRRSNGTINRPLLKIFDVKKSPSKKPINGVKTTDITIDKSR 69

Query: 74  DLFLRIFTPTAAAAAADSLPAPVIFYFHGGGFAIGSADSTSMDLAATRFAKELRAVVISV 133
           DL+ R++TPT    + DS   PVIFYFHGGGF++ +A+S  MD    R A+EL A +ISV
Sbjct: 70  DLWFRLYTPTTNTTS-DSAGLPVIFYFHGGGFSLLAANSRFMDDFCYRLARELSAFIISV 129

Query: 134 NYRLAPEFRFPCQYDDGFDALKFIDEMDDSALMGRADLSHCFILGESAGGNLGHHVAVRA 193
           NYRLAPE RFP QYDDGFDALK+ID  +       A+L+HCF+ G+SAGGNL HHV ++A
Sbjct: 130 NYRLAPEHRFPSQYDDGFDALKYIDSTEIEGFSSYANLNHCFVAGDSAGGNLAHHVIIKA 189

Query: 194 SEYAFKRVKVVGFIASQPFFGGEERTESEIRLCNEPLLTLKITDWFWKAFLPAGENRDHA 253
           +EY F  +K++G I+ QPF GGEERTESE+RL N P +T++ TDW W+AFLP G NRDH 
Sbjct: 190 NEYEFSNIKLIGNISFQPFLGGEERTESELRLTNAPFITIERTDWMWRAFLPEGSNRDHP 249

Query: 254 AVNVNGPNGRDILGVRNFPATVIFAGELDLLIDRHKSYYEWLMKMEKDVKLVVFSNAVHG 313
           AVN+ GPNG DI G++ FPAT++  G  D L D  K YYE L    K+  LV + N  H 
Sbjct: 250 AVNIFGPNGSDISGMK-FPATIVVMGGYDPLQDWQKRYYEGLKMSGKEAYLVEYPNMPHS 309

Query: 314 FSVFADLPEYSLMVKEIKDFIAK 337
           F ++ +LPE  L ++++KDF+ K
Sbjct: 310 FYIYNELPESGLSIEKVKDFMQK 330

BLAST of Lsi10G006950 vs. TrEMBL
Match: V4UJX3_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10026049mg PE=4 SV=1)

HSP 1 Score: 339.0 bits (868), Expect = 6.8e-90
Identity = 168/323 (52.01%), Postives = 219/323 (67.80%), Query Frame = 1

Query: 15  LPWKHKLTLRVAALLFNTFLRSNLTVNRWLLNFFDPKIPPNPNLNHAVSSSDLTIDASRD 74
           +PW  +L L   + + ++  R + T+NR L+NF D K  PNPN  ++VS+SD+T+D SR 
Sbjct: 11  IPWTTRLALTFVSAIGDSGRRPDGTINRRLMNFLDVKSRPNPNPVNSVSTSDVTVDPSRP 70

Query: 75  LFLRIFTPTAAAAAADSLPA-PVIFYFHGGGFAIGSADSTSMDLAATRFAKELRAVVISV 134
           L+ R+FTPT      DS P+ PV+ +FHGGGF   SA S S D    RFA++  A V+SV
Sbjct: 71  LWFRLFTPT------DSTPSLPVLIFFHGGGFTYLSAASKSYDAVCRRFARKFPAFVVSV 130

Query: 135 NYRLAPEFRFPCQYDDGFDALKFIDEMDDSALMGRADLSHCFILGESAGGNLGHHVAVRA 194
           NYRL PE R+P QYDDGFD L+F+D+  DS L   ADLS CF+ G+SAG NL HHVA+RA
Sbjct: 131 NYRLCPEHRYPSQYDDGFDVLRFLDDHRDSVLPPNADLSRCFLAGDSAGANLAHHVALRA 190

Query: 195 SEYAFKRVKVVGFIASQPFFGGEERTESEIRLCNEPLLTLKITDWFWKAFLPAGENRDHA 254
           S   F+ VK++G ++ QPFFGGEERTESEIRL   PL++   TDW WKAFLP G NRDH 
Sbjct: 191 SGSPFRFVKLLGLVSIQPFFGGEERTESEIRLSGSPLVSTARTDWIWKAFLPDGSNRDHE 250

Query: 255 AVNVNGPNGRDILGVRNFPATVIFAGELDLLIDRHKSYYEWLMKMEKDVKLVVFSNAVHG 314
           A NV+GPN  D+ G+  +P TV+  G  D L D  + YYEWL K  K+  L+ + N +H 
Sbjct: 251 ASNVSGPNAVDVSGL-EYPDTVVVVGGFDPLQDWQRRYYEWLKKSGKEATLIEYPNMIHA 310

Query: 315 FSVFADLPEYSLMVKEIKDFIAK 337
           F VF +LPE S ++ +IKDFI+K
Sbjct: 311 FYVFPELPEASQLISQIKDFISK 326

BLAST of Lsi10G006950 vs. TAIR10
Match: AT5G23530.1 (AT5G23530.1 carboxyesterase 18)

HSP 1 Score: 290.8 bits (743), Expect = 1.1e-78
Identity = 153/323 (47.37%), Postives = 203/323 (62.85%), Query Frame = 1

Query: 15  LPWKHKLTLRVAALLFNTFLRSNLTVNRWLLNFFDPKIPPNPNLNHAVSSSDLTIDASRD 74
           LP K ++ L V + + +   R + T+NR  L  FD + PPNP   + VS+SD  +D SRD
Sbjct: 13  LPLKTRIALTVISTMTDNAQRPDGTINRRFLRLFDFRAPPNPKPVNIVSTSDFVVDQSRD 72

Query: 75  LFLRIFTPTAAAAAADSLPAPVIFYFHGGGFAIGSADSTSMDLAATRFAKELRAVVISVN 134
           L+ R++TP  +    D +P  V+ +FHGGGFA  S ++   D    RFA++L A VISVN
Sbjct: 73  LWFRLYTPHVSG---DKIP--VVVFFHGGGFAFLSPNAYPYDNVCRRFARKLPAYVISVN 132

Query: 135 YRLAPEFRFPCQYDDGFDALKFIDEMDDSALMGRADLSHCFILGESAGGNLGHHVAV--- 194
           YRLAPE R+P QYDDGFDALK+I+E   S L   ADLS CF  G+SAGGN+ H+VA+   
Sbjct: 133 YRLAPEHRYPAQYDDGFDALKYIEENHGSILPANADLSRCFFAGDSAGGNIAHNVAIRIC 192

Query: 195 RASEYAFKRVKVVGFIASQPFFGGEERTESEIRLCNEPLLTLKITDWFWKAFLPAGENRD 254
           R    +F  VK++G I+ QPFFGGEERTE+E +L   PL++   TDW WKA    G NRD
Sbjct: 193 REPRSSFTAVKLIGLISIQPFFGGEERTEAEKQLVGAPLVSPDRTDWCWKAM---GLNRD 252

Query: 255 HAAVNVNGPNGRDILGVRNFPATVIFAGELDLLIDRHKSYYEWLMKMEKDVKLVVFSNAV 314
           H AVNV GPN  DI G+ ++P T++     D L D  +SYYEWL    K   L+ + N  
Sbjct: 253 HEAVNVGGPNAVDISGL-DYPETMVVVAGFDPLKDWQRSYYEWLKLCGKKATLIEYPNMF 312

Query: 315 HGFSVFADLPEYSLMVKEIKDFI 335
           H F +F +LPE   ++  IKDF+
Sbjct: 313 HAFYIFPELPEAGQLIMRIKDFV 326

BLAST of Lsi10G006950 vs. TAIR10
Match: AT5G27320.1 (AT5G27320.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 212.6 bits (540), Expect = 3.7e-55
Identity = 124/317 (39.12%), Postives = 175/317 (55.21%), Query Frame = 1

Query: 28  LLFNTFLRSNLTVNRWLLNFFDPKIPPNPNLNHAVSSSDLTIDASRDLFLRIFTPTAAAA 87
           L +N   R + T NR L  F D K+P N N  + V S D+ ID   +L  R++ P  A  
Sbjct: 29  LAYNLLRRPDGTFNRHLAEFLDRKVPANANPVNGVFSFDVIIDRQTNLLSRVYRPADAGT 88

Query: 88  A---------ADSLPAPVIFYFHGGGFAIGSADSTSMDLAATRFAKELRAVVISVNYRLA 147
           +          D    PVI +FHGG FA  SA+S   D    R      AVV+SVNYR A
Sbjct: 89  SPSITDLQNPVDGEIVPVIVFFHGGSFAHSSANSAIYDTLCRRLVGLCGAVVVSVNYRRA 148

Query: 148 PEFRFPCQYDDGFDALKFIDEMDDSALMGRADLS-HCFILGESAGGNLGHHVAVRASEYA 207
           PE R+PC YDDG+  LK+++    S L  + D     F+ G+S+GGN+ H+VAVRA E  
Sbjct: 149 PENRYPCAYDDGWAVLKWVNS--SSWLRSKKDSKVRIFLAGDSSGGNIVHNVAVRAVE-- 208

Query: 208 FKRVKVVGFIASQPFFGGEERTESEIRLCNEPLLTLKITDWFWKAFLPAGENRDHAAVNV 267
             R+ V+G I   P FGG ERTESE RL  +  +T++  DW+W+AFLP GE+R+H A + 
Sbjct: 209 -SRIDVLGNILLNPMFGGTERTESEKRLDGKYFVTVRDRDWYWRAFLPEGEDREHPACSP 268

Query: 268 NGPNGRDILGVRNFPATVIFAGELDLLIDRHKSYYEWLMKMEKDVKLVVFSNAVHGFSVF 327
            GP  + + G+ +FP +++    LDL+ D    Y E L K  ++VKL+    A  GF + 
Sbjct: 269 FGPRSKSLEGL-SFPKSLVVVAGLDLIQDWQLKYAEGLKKAGQEVKLLYLEQATIGFYLL 328

Query: 328 ADLPEYSLMVKEIKDFI 335
            +   +  ++ EI  F+
Sbjct: 329 PNNNHFHTVMDEIAAFV 339

BLAST of Lsi10G006950 vs. TAIR10
Match: AT3G05120.1 (AT3G05120.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 202.6 bits (514), Expect = 3.9e-52
Identity = 120/317 (37.85%), Postives = 170/317 (53.63%), Query Frame = 1

Query: 30  FNTFLRSNLTVNRWLLNFFDPKIPPNPNLNHAVSSSDLTIDASRDLFLRIFTPTAA---- 89
           +N   R + T NR L  + D K+  N N    V S D+ ID   +L  R++ P  A    
Sbjct: 31  YNILRRPDGTFNRHLAEYLDRKVTANANPVDGVFSFDVLIDRRINLLSRVYRPAYADQEQ 90

Query: 90  -------AAAADSLPAPVIFYFHGGGFAIGSADSTSMDLAATRFAKELRAVVISVNYRLA 149
                      D    PVI +FHGG FA  SA+S   D    R     + VV+SVNYR A
Sbjct: 91  PPSILDLEKPVDGDIVPVILFFHGGSFAHSSANSAIYDTLCRRLVGLCKCVVVSVNYRRA 150

Query: 150 PEFRFPCQYDDGFDALKFIDEMDDSALMGRADLS-HCFILGESAGGNLGHHVAVRASEYA 209
           PE  +PC YDDG+ AL +++    S L  + D   H F+ G+S+GGN+ H+VA+RA E  
Sbjct: 151 PENPYPCAYDDGWIALNWVNSR--SWLKSKKDSKVHIFLAGDSSGGNIAHNVALRAGESG 210

Query: 210 FKRVKVVGFIASQPFFGGEERTESEIRLCNEPLLTLKITDWFWKAFLPAGENRDHAAVNV 269
              + V+G I   P FGG ERTESE  L  +  +T++  DW+WKAFLP GE+R+H A N 
Sbjct: 211 ---IDVLGNILLNPMFGGNERTESEKSLDGKYFVTVRDRDWYWKAFLPEGEDREHPACNP 270

Query: 270 NGPNGRDILGVRNFPATVIFAGELDLLIDRHKSYYEWLMKMEKDVKLVVFSNAVHGFSVF 329
             P G+ + GV +FP +++    LDL+ D   +Y E L K  ++VKL+    A  GF + 
Sbjct: 271 FSPRGKSLEGV-SFPKSLVVVAGLDLIRDWQLAYAEGLKKAGQEVKLMHLEKATVGFYLL 330

Query: 330 ADLPEYSLMVKEIKDFI 335
            +   +  ++ EI  F+
Sbjct: 331 PNNNHFHNVMDEISAFV 341

BLAST of Lsi10G006950 vs. TAIR10
Match: AT3G63010.1 (AT3G63010.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 189.9 bits (481), Expect = 2.6e-48
Identity = 110/319 (34.48%), Postives = 171/319 (53.61%), Query Frame = 1

Query: 28  LLFNTFLRSNLTVNRWLLNFFDPKIPPNPNLNHAVSSSDLTIDASRDLFLRIFTPTA--- 87
           L +    R + + NR L  F D K+P N      V S D  +D++ +L  RI+ P +   
Sbjct: 29  LAYKVLRRPDGSFNRDLAEFLDRKVPANSFPLDGVFSFD-HVDSTTNLLTRIYQPASLLH 88

Query: 88  ---------AAAAADSLPAPVIFYFHGGGFAIGSADSTSMDLAATRFAKELRAVVISVNY 147
                        + +   PV+ +FHGG F   SA+S   D    R       VV+SV+Y
Sbjct: 89  QTRHGTLELTKPLSTTEIVPVLIFFHGGSFTHSSANSAIYDTFCRRLVTICGVVVVSVDY 148

Query: 148 RLAPEFRFPCQYDDGFDALKFIDEMDDSALMGRADLSHCFILGESAGGNLGHHVAVRASE 207
           R +PE R+PC YDDG++AL ++         G+    + ++ G+S+GGN+ H+VAVRA+ 
Sbjct: 149 RRSPEHRYPCAYDDGWNALNWVKSRV-WLQSGKDSNVYVYLAGDSSGGNIAHNVAVRATN 208

Query: 208 YAFKRVKVVGFIASQPFFGGEERTESEIRLCNEPLLTLKITDWFWKAFLPAGENRDHAAV 267
              + VKV+G I   P FGG+ERT+SE  L  +  +T++  DW+W+A+LP GE+RDH A 
Sbjct: 209 ---EGVKVLGNILLHPMFGGQERTQSEKTLDGKYFVTIQDRDWYWRAYLPEGEDRDHPAC 268

Query: 268 NVNGPNGRDILGVRNFPATVIFAGELDLLIDRHKSYYEWLMKMEKDVKLVVFSNAVHGFS 327
           N  GP G+ + GV NFP +++    LDL+ D   +Y + L K   +V L+    A  GF 
Sbjct: 269 NPFGPRGQSLKGV-NFPKSLVVVAGLDLVQDWQLAYVDGLKKTGLEVNLLYLKQATIGFY 328

Query: 328 VFADLPEYSLMVKEIKDFI 335
              +   +  +++E+  F+
Sbjct: 329 FLPNNDHFHCLMEELNKFV 341

BLAST of Lsi10G006950 vs. TAIR10
Match: AT5G62180.1 (AT5G62180.1 carboxyesterase 20)

HSP 1 Score: 171.8 bits (434), Expect = 7.3e-43
Identity = 105/302 (34.77%), Postives = 159/302 (52.65%), Query Frame = 1

Query: 39  TVNRWLLNFFDPKIPPNPNLNHAVSSSDLTIDASRDLFLRIFTPTAAAAAAD--SLPAPV 98
           ++ R L NF      P+P+  +   S DL ++  +  +LR++ P++A    +  S   P+
Sbjct: 23  SITRDLSNFPCTAATPDPSPLNPAVSKDLPVNQLKSTWLRLYLPSSAVNEGNVSSQKLPI 82

Query: 99  IFYFHGGGFAIGSADSTSMDLAATRFAKELRAVVISVNYRLAPEFRFPCQYDDGFDALKF 158
           + Y+HGGGF + S D        +  A++L A+V+S +YRLAPE R P  YDDG +AL +
Sbjct: 83  VVYYHGGGFILCSVDMQLFHDFCSEVARDLNAIVVSPSYRLAPEHRLPAAYDDGVEALDW 142

Query: 159 IDEMDDSALMGRADLSHCFILGESAGGNLGHHVAVRA--SEYAFKRVKVVGFIASQPFFG 218
           I   DD  +   AD S+ F++G SAGGNL ++V +R+  S      +++ G I   PFFG
Sbjct: 143 IKTSDDEWIKSHADFSNVFLMGTSAGGNLAYNVGLRSVDSVSDLSPLQIRGLILHHPFFG 202

Query: 219 GEERTESEIRLCNEPLLTLKITDWFWKAFLPAGENRDHAAVNVNGPNGRDILGV--RNFP 278
           GEER+ESEIRL N+ +    +TD  W   LP G +RDH   N    +G + L    R   
Sbjct: 203 GEERSESEIRLMNDQVCPPIVTDVMWDLSLPVGVDRDHEYSNPTVGDGSEKLEKIGRLRW 262

Query: 279 ATVIFAGELDLLIDRHKSYYEWLMKMEKDVKLVVFSNAVHGFSVFADLPEYSLMVKEIKD 335
             ++  GE D +ID  K   + + K   +V        VHG  +  D  +   +   IK+
Sbjct: 263 KVMMIGGEDDPMIDLQKDVAKLMKKKGVEVVEHYTGGHVHGAEI-RDPSKRKTLFLSIKN 322

BLAST of Lsi10G006950 vs. NCBI nr
Match: gi|449447725|ref|XP_004141618.1| (PREDICTED: probable carboxylesterase 18 [Cucumis sativus])

HSP 1 Score: 530.0 bits (1364), Expect = 3.0e-147
Identity = 263/338 (77.81%), Postives = 289/338 (85.50%), Query Frame = 1

Query: 1   MWASSSYLEISTSQLPWKHKLTLRVAALLFNTFLRSNLTVNRWLLNFFDPKIPPNPNLNH 60
           M +SS   E+ST QLPWKHK+TLR A LLFNT LRS+ TVNR LL F DPKIPPNPN  H
Sbjct: 1   MRSSSVCYELSTPQLPWKHKVTLRFATLLFNTSLRSDFTVNRRLLTFLDPKIPPNPNSAH 60

Query: 61  AVSSSDLTIDASRDLFLRIFTPTAAAAAADSLPA-PVIFYFHGGGFAIGSADSTSMDLAA 120
           +VSSSDLTID SRDLFLRIFTP   AA  +SLP  P+IFYFHGGGFA GSAD+TS D+AA
Sbjct: 61  SVSSSDLTIDTSRDLFLRIFTPNPTAALDESLPLLPIIFYFHGGGFAFGSADATSTDMAA 120

Query: 121 TRFAKELRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDSALMGRADLSHCFILGE 180
             FA++LRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDD +L+ R DLS CFILGE
Sbjct: 121 RGFAEKLRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDSLLERVDLSRCFILGE 180

Query: 181 SAGGNLGHHVAVRASEYAFKRVKVVGFIASQPFFGGEERTESEIRLCNEPLLTLKITDWF 240
           SAGGNLGHHVAVRASEY FKRVK++GFIASQPFFGG+ERTESE RLC +  LTL +TDWF
Sbjct: 181 SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLYMTDWF 240

Query: 241 WKAFLPAGENRDHAAVNVNGPNGRDILGVRNFPATVIFAGELDLLIDRHKSYYEWLMKME 300
           W+AFLPAGE+RDHAA NVNGPNGRDI G+ NFPATVIFAG LDLL+DR KSYYE L +M 
Sbjct: 241 WRAFLPAGEDRDHAAANVNGPNGRDISGLENFPATVIFAGGLDLLMDRQKSYYERLKRMG 300

Query: 301 KDVKLVVFSNAVHGFSVFADLPEYSLMVKEIKDFIAKL 338
           KDVKLVVFSNA HGF  F DLPEYSLM++E+ DFIAKL
Sbjct: 301 KDVKLVVFSNAFHGFFGFPDLPEYSLMIEEMSDFIAKL 338

BLAST of Lsi10G006950 vs. NCBI nr
Match: gi|659119543|ref|XP_008459713.1| (PREDICTED: probable carboxylesterase 18 [Cucumis melo])

HSP 1 Score: 525.4 bits (1352), Expect = 7.4e-146
Identity = 259/337 (76.85%), Postives = 289/337 (85.76%), Query Frame = 1

Query: 1   MWASSSYLEISTSQLPWKHKLTLRVAALLFNTFLRSNLTVNRWLLNFFDPKIPPNPNLNH 60
           M +S    +IST QLPWKH++TLR A LL+NT LRS+LTVNR LLNF DPKIP N +  H
Sbjct: 1   MCSSGVCYQISTPQLPWKHRITLRFATLLYNTSLRSDLTVNRRLLNFIDPKIPSNHDSTH 60

Query: 61  AVSSSDLTIDASRDLFLRIFTPTAAAAAADSLPA-PVIFYFHGGGFAIGSADSTSMDLAA 120
           +VSSSDL +D SRDLFLRIFTPT  AA  +SLP  P+IFYFHGGGF  GSAD+TS D+AA
Sbjct: 61  SVSSSDLIVDTSRDLFLRIFTPTPTAALDESLPLLPIIFYFHGGGFVFGSADATSTDMAA 120

Query: 121 TRFAKELRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDSALMGRADLSHCFILGE 180
            RFAK+LRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDD +L+ R DLS CFILGE
Sbjct: 121 RRFAKKLRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDSLLERVDLSCCFILGE 180

Query: 181 SAGGNLGHHVAVRASEYAFKRVKVVGFIASQPFFGGEERTESEIRLCNEPLLTLKITDWF 240
           SAGGNLGHHVAVRASEY FKRVK++GFIASQPFFGG+ERTESE RLC +  LTL +TDWF
Sbjct: 181 SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLDMTDWF 240

Query: 241 WKAFLPAGENRDHAAVNVNGPNGRDILGVRNFPATVIFAGELDLLIDRHKSYYEWLMKME 300
           W+AFLPAGE+RDHAA NVNGPNGRDI G+ NFPAT+IFAG LDLLIDR K YYE L +M 
Sbjct: 241 WRAFLPAGEDRDHAAANVNGPNGRDISGLENFPATLIFAGGLDLLIDRQKCYYERLKRMG 300

Query: 301 KDVKLVVFSNAVHGFSVFADLPEYSLMVKEIKDFIAK 337
           KDVKLVVFSNAVHG+  FADLPEYSLM+KE++DFIAK
Sbjct: 301 KDVKLVVFSNAVHGYFGFADLPEYSLMIKEMRDFIAK 337

BLAST of Lsi10G006950 vs. NCBI nr
Match: gi|449447721|ref|XP_004141616.1| (PREDICTED: probable carboxylesterase 18 [Cucumis sativus])

HSP 1 Score: 432.6 bits (1111), Expect = 6.5e-118
Identity = 212/335 (63.28%), Postives = 254/335 (75.82%), Query Frame = 1

Query: 1   MWASSSYLEISTSQLPWKHKLTLRVAALLFNTFLRSNLTVNRWLLNFFDPKIPPNPNLNH 60
           M +S+S  EIS+S+LPWKH+L LR+ + + +   RS+ TVNRW     D KIPP+     
Sbjct: 1   MSSSTSASEISSSKLPWKHRLLLRIGSTITDACCRSDFTVNRWFTGILDWKIPPSTKPID 60

Query: 61  AVSSSDLTIDASRDLFLRIFTPTAAAAAADSLPAPVIFYFHGGGFAIGSADSTSMDLAAT 120
            VSS DLTID SR+L++RIF P      +D    P+IFYFHGGGFA   ADS     +A 
Sbjct: 61  GVSSFDLTIDTSRNLWVRIFNPVIDGEDSDIQSLPLIFYFHGGGFAFSYADSALSHTSAH 120

Query: 121 RFAKELRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDSALMGRADLSHCFILGES 180
           RFAK+L AVVISVNYRLAPEFR+PCQYDDGFDALKFIDE+ +  L  +ADL+ CFILGES
Sbjct: 121 RFAKQLPAVVISVNYRLAPEFRYPCQYDDGFDALKFIDEVGEEILPAKADLTRCFILGES 180

Query: 181 AGGNLGHHVAVRASEYAFKRVKVVGFIASQPFFGGEERTESEIRLCNEPLLTLKITDWFW 240
           AGGNLGHHVAVRASEY  K+VK+VGFIASQPFFGGEERTESEIRL N+  L+L+++DWFW
Sbjct: 181 AGGNLGHHVAVRASEYTLKKVKMVGFIASQPFFGGEERTESEIRLSNQRPLSLRLSDWFW 240

Query: 241 KAFLPAGENRDHAAVNVNGPNGRDILGVRNFPATVIFAGELDLLIDRHKSYYEWLMKMEK 300
           KAFLP GE+RDH A NV GP GRD+  V  FPAT++  GELDLL D  + YYE L +M K
Sbjct: 241 KAFLPEGEDRDHGAANVFGPKGRDVTEVMKFPATLVMVGELDLLQDGQRRYYEGLKRMGK 300

Query: 301 DVKLVVFSNAVHGFSVFADLPEYSLMVKEIKDFIA 336
           +VK+V F NA+HGF  F DLP+YS M+KE+KDFIA
Sbjct: 301 EVKMVEFENAIHGFFAFWDLPQYSSMMKEMKDFIA 335

BLAST of Lsi10G006950 vs. NCBI nr
Match: gi|659119541|ref|XP_008459712.1| (PREDICTED: probable carboxylesterase 18 [Cucumis melo])

HSP 1 Score: 430.3 bits (1105), Expect = 3.2e-117
Identity = 212/333 (63.66%), Postives = 253/333 (75.98%), Query Frame = 1

Query: 3   ASSSYLEISTSQLPWKHKLTLRVAALLFNTFLRSNLTVNRWLLNFFDPKIPPNPNLNHAV 62
           +S S  EIS+S+LPWKH+L LR+  ++ +   RS+ TVNRWL    D KIPP+      V
Sbjct: 4   SSISACEISSSKLPWKHRLLLRIGTIITDACCRSDFTVNRWLSGTLDWKIPPSTKPIDGV 63

Query: 63  SSSDLTIDASRDLFLRIFTPTAAAAAADSLPAPVIFYFHGGGFAIGSADSTSMDLAATRF 122
           SS DLTID SR+L++RIF P      ++    P+IFYFHGGGFA   ADS     +A RF
Sbjct: 64  SSFDLTIDTSRNLWVRIFNPIIDDEDSNLQSLPLIFYFHGGGFAFSYADSALSHTSAHRF 123

Query: 123 AKELRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDSALMGRADLSHCFILGESAG 182
           AK+L AVVISVNYRLAPEFR+PCQYDDGFDALKFIDE+ +  L  +ADLS CFILGESAG
Sbjct: 124 AKQLPAVVISVNYRLAPEFRYPCQYDDGFDALKFIDEVGEEILPAKADLSRCFILGESAG 183

Query: 183 GNLGHHVAVRASEYAFKRVKVVGFIASQPFFGGEERTESEIRLCNEPLLTLKITDWFWKA 242
           GNLGHHVAVRASEY  K+V++VGFIASQPFFGGEERTESEIRL N+  L L+++DWFWKA
Sbjct: 184 GNLGHHVAVRASEYTLKKVRLVGFIASQPFFGGEERTESEIRLRNQRPLNLRLSDWFWKA 243

Query: 243 FLPAGENRDHAAVNVNGPNGRDILGVRNFPATVIFAGELDLLIDRHKSYYEWLMKMEKDV 302
           FLP GE+RDHAA NV GP GRD+  V  FPAT++  GELDLL D  + YYE L +M K+V
Sbjct: 244 FLPEGEDRDHAAANVFGPKGRDVREVMKFPATLVLVGELDLLQDGQRRYYEGLKRMGKEV 303

Query: 303 KLVVFSNAVHGFSVFADLPEYSLMVKEIKDFIA 336
           K+V F NA+HGF  F DLP+YS M+KE+KDFIA
Sbjct: 304 KMVEFENAIHGFFAFWDLPQYSSMIKEMKDFIA 336

BLAST of Lsi10G006950 vs. NCBI nr
Match: gi|802646940|ref|XP_012079703.1| (PREDICTED: probable carboxylesterase 18 [Jatropha curcas])

HSP 1 Score: 348.6 bits (893), Expect = 1.2e-92
Identity = 171/323 (52.94%), Postives = 222/323 (68.73%), Query Frame = 1

Query: 14  QLPWKHKLTLRVAALLFNTFLRSNLTVNRWLLNFFDPKIPPNPNLNHAVSSSDLTIDASR 73
           QLPWK  L + V +   +   RSN T+NR LL  FD K  P+    + V ++D+TID SR
Sbjct: 10  QLPWKANLFISVMSFGVDIVRRSNGTINRPLLKIFDVKKSPSKKPINGVKTTDITIDKSR 69

Query: 74  DLFLRIFTPTAAAAAADSLPAPVIFYFHGGGFAIGSADSTSMDLAATRFAKELRAVVISV 133
           DL+ R++TPT    + DS   PVIFYFHGGGF++ +A+S  MD    R A+EL A +ISV
Sbjct: 70  DLWFRLYTPTTNTIS-DSAGLPVIFYFHGGGFSLLAANSRPMDDFCYRLARELSAFIISV 129

Query: 134 NYRLAPEFRFPCQYDDGFDALKFIDEMDDSALMGRADLSHCFILGESAGGNLGHHVAVRA 193
           NYRLAPE RFP QYDDGFDALK+ID  +       A+L+HCF+ G+SAGGNL HHV ++A
Sbjct: 130 NYRLAPEHRFPSQYDDGFDALKYIDSTEIEGFSSYANLNHCFVAGDSAGGNLAHHVIIKA 189

Query: 194 SEYAFKRVKVVGFIASQPFFGGEERTESEIRLCNEPLLTLKITDWFWKAFLPAGENRDHA 253
           +EY F  +K++G I+ QPF GGEERTESE+RL N P +T++ TDW W+AFLP G NRDH 
Sbjct: 190 NEYEFSNIKLIGNISFQPFLGGEERTESELRLTNAPFITIERTDWMWRAFLPGGSNRDHP 249

Query: 254 AVNVNGPNGRDILGVRNFPATVIFAGELDLLIDRHKSYYEWLMKMEKDVKLVVFSNAVHG 313
           AVN+ GPNG DI G++ FPAT++F G  D L D  K YYE L K  K+  LV + N  H 
Sbjct: 250 AVNIFGPNGVDISGMK-FPATIVFVGGYDPLQDWQKRYYEGLKKSGKEAYLVEYPNMPHS 309

Query: 314 FSVFADLPEYSLMVKEIKDFIAK 337
           F ++ +LPE  L ++E+KDF+ K
Sbjct: 310 FYIYNELPESGLSIEEVKDFMQK 330

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
CXE18_ARATH1.9e-7747.37Probable carboxylesterase 18 OS=Arabidopsis thaliana GN=CXE18 PE=1 SV=1[more]
GID1C_ARATH6.6e-5439.12Gibberellin receptor GID1C OS=Arabidopsis thaliana GN=GID1C PE=1 SV=1[more]
GID1_ORYSJ1.1e-5340.31Gibberellin receptor GID1 OS=Oryza sativa subsp. japonica GN=GID1 PE=1 SV=1[more]
GID1A_ARATH6.8e-5137.85Gibberellin receptor GID1A OS=Arabidopsis thaliana GN=GID1A PE=1 SV=1[more]
GID1B_ARATH4.6e-4734.48Gibberellin receptor GID1B OS=Arabidopsis thaliana GN=GID1B PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KXS8_CUCSA2.1e-14777.81Acetyl esterase OS=Cucumis sativus GN=Csa_5G647390 PE=4 SV=1[more]
A0A0A0KUP3_CUCSA4.5e-11863.28Uncharacterized protein OS=Cucumis sativus GN=Csa_5G647370 PE=4 SV=1[more]
A0A067KH58_JATCU8.6e-9352.94Uncharacterized protein OS=Jatropha curcas GN=JCGZ_14820 PE=4 SV=1[more]
A0A067K634_JATCU8.0e-9152.01Uncharacterized protein OS=Jatropha curcas GN=JCGZ_14822 PE=4 SV=1[more]
V4UJX3_9ROSI6.8e-9052.01Uncharacterized protein OS=Citrus clementina GN=CICLE_v10026049mg PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G23530.11.1e-7847.37 carboxyesterase 18[more]
AT5G27320.13.7e-5539.12 alpha/beta-Hydrolases superfamily protein[more]
AT3G05120.13.9e-5237.85 alpha/beta-Hydrolases superfamily protein[more]
AT3G63010.12.6e-4834.48 alpha/beta-Hydrolases superfamily protein[more]
AT5G62180.17.3e-4334.77 carboxyesterase 20[more]
Match NameE-valueIdentityDescription
gi|449447725|ref|XP_004141618.1|3.0e-14777.81PREDICTED: probable carboxylesterase 18 [Cucumis sativus][more]
gi|659119543|ref|XP_008459713.1|7.4e-14676.85PREDICTED: probable carboxylesterase 18 [Cucumis melo][more]
gi|449447721|ref|XP_004141616.1|6.5e-11863.28PREDICTED: probable carboxylesterase 18 [Cucumis sativus][more]
gi|659119541|ref|XP_008459712.1|3.2e-11763.66PREDICTED: probable carboxylesterase 18 [Cucumis melo][more]
gi|802646940|ref|XP_012079703.1|1.2e-9252.94PREDICTED: probable carboxylesterase 18 [Jatropha curcas][more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0016787hydrolase activity
Vocabulary: Biological Process
TermDefinition
GO:0008152metabolic process
Vocabulary: INTERPRO
TermDefinition
IPR013094AB_hydrolase_3
IPR002168Lipase_GDXG_HIS_AS
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008152 metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0016787 hydrolase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi10G006950.1Lsi10G006950.1mRNA


Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002168Lipase, GDXG, putative histidine active sitePROSITEPS01173LIPASE_GDXG_HIScoord: 97..113
scor
IPR013094Alpha/beta hydrolase fold-3PFAMPF07859Abhydrolase_3coord: 97..314
score: 6.9
NoneNo IPR availablePANTHERPTHR23024MEMBER OF 'GDXG' FAMILY OF LIPOLYTIC ENZYMEScoord: 24..336
score: 1.9E
NoneNo IPR availablePANTHERPTHR23024:SF174SUBFAMILY NOT NAMEDcoord: 24..336
score: 1.9E