Lsi10G006900 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi10G006900
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Terpene synthase
Location	chr10 : 9673719 .. 9674686 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AATCAAACAAGGCTTCAAATATACCTATCTTTCTCTCATATGGCTCTTCACCATGTTCTTGGTTCTTCCTTGGTGAGATCTTTCATCAAACAAGTTTCTTTTCATCCTAAACCTTCTTTTGTTCCTCGTATTGCTAAAATAAAGGGGACTACAAAAATATCGGAGGATGATCAGATTATCGTTCGACGATCTGCAAATTACCAACCTCCTATATGGAAATACGAGTTTGTCCAATCACTGACGAGTGAGTTTAAGGTATATAATATGAGTTGAAATTATGTATTAGTTGTTTAGGTGTACTTCGTTAGTAATTAGGTTTAGTTATAGAATTATTTTGTGACCTATTTCATGTGATTTTCAGGGAGAAACATGCTCAAGACGTTTCAATGAGCTAAAGAAAAAGGTTGAAGTCCTGATGAGACGAATAGTTGATGACCCATTAAAACAACTCGAGTTCATTGACATTTTGCAACGACTTGGAATATCATACCACTTTGAGAATGAGATTAAGGATGTCTTAGAAAGAATATACAACAAGAGTTATGAAGATGGCGATAGGTTGAATAAGAGTTCTCTTTATCAAACATCCCTTGAATTTAGACTCTTGAGACAACATGGATTCAATGTTTCAGAAGGTATTGATTCATTCTTTTTTATTAACGTCCATTATATTACACATAATCATTCAAAATATTATAATATAGACATCATTTATTTTCTTATACTTGTAATACACACACTATTTAATTTAATAAAAGTGAGTTACTATAATGAAGAGATCTCCACATCTCAACATTTCTTATAGTAGAAAATTTGAAATAAAATCTTATGAGATAAAACTATTACAACTTTTTGTTAAAGGAAAAGAGAATATATGAAGTTATGTATAAAAAAAAAAGAATTTATGTATATTAAGTTATAATGTACTAGAATTGATCTCTTCGAAAAAGTTCATCCTAACAATTTAA

mRNA sequence

AATCAAACAAGGCTTCAAATATACCTATCTTTCTCTCATATGGCTCTTCACCATGTTCTTGGTTCTTCCTTGGTGAGATCTTTCATCAAACAAGTTTCTTTTCATCCTAAACCTTCTTTTGTTCCTCGTATTGCTAAAATAAAGGGGACTACAAAAATATCGGAGGATGATCAGATTATCGTTCGACGATCTGCAAATTACCAACCTCCTATATGGAAATACGAGTTTGTCCAATCACTGACGAGTGAGTTTAAGGGAGAAACATGCTCAAGACGTTTCAATGAGCTAAAGAAAAAGGTTGAAGTCCTGATGAGACGAATAGTTGATGACCCATTAAAACAACTCGAGTTCATTGACATTTTGCAACGACTTGGAATATCATACCACTTTGAGAATGAGATTAAGGATGTCTTAGAAAGAATATACAACAAGAGTTATGAAGATGGCGATAGGTTGAATAAGAGTTCTCTTTATCAAACATCCCTTGAATTTAGACTCTTGAGACAACATGGATTCAATGTTTCAGAAGAATTGATCTCTTCGAAAAAGTTCATCCTAACAATTTAA

Coding sequence (CDS)

ATGGCTCTTCACCATGTTCTTGGTTCTTCCTTGGTGAGATCTTTCATCAAACAAGTTTCTTTTCATCCTAAACCTTCTTTTGTTCCTCGTATTGCTAAAATAAAGGGGACTACAAAAATATCGGAGGATGATCAGATTATCGTTCGACGATCTGCAAATTACCAACCTCCTATATGGAAATACGAGTTTGTCCAATCACTGACGAGTGAGTTTAAGGGAGAAACATGCTCAAGACGTTTCAATGAGCTAAAGAAAAAGGTTGAAGTCCTGATGAGACGAATAGTTGATGACCCATTAAAACAACTCGAGTTCATTGACATTTTGCAACGACTTGGAATATCATACCACTTTGAGAATGAGATTAAGGATGTCTTAGAAAGAATATACAACAAGAGTTATGAAGATGGCGATAGGTTGAATAAGAGTTCTCTTTATCAAACATCCCTTGAATTTAGACTCTTGAGACAACATGGATTCAATGTTTCAGAAGAATTGATCTCTTCGAAAAAGTTCATCCTAACAATTTAA

Protein sequence

MALHHVLGSSLVRSFIKQVSFHPKPSFVPRIAKIKGTTKISEDDQIIVRRSANYQPPIWKYEFVQSLTSEFKGETCSRRFNELKKKVEVLMRRIVDDPLKQLEFIDILQRLGISYHFENEIKDVLERIYNKSYEDGDRLNKSSLYQTSLEFRLLRQHGFNVSEELISSKKFILTI

Homology

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BLAST of Lsi10G006900 vs. Swiss-Prot
Match: MYRS_QUEIL (Myrcene synthase, chloroplastic OS=Quercus ilex PE=1 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 2.2e-32
Identity = 70/152 (46.05%), Postives = 108/152 (71.05%), Query Frame = 1


Query: 19  VSFHPKPSFVPRIAKIKGTTKISEDDQIIVRRSANYQPPIWKYEFVQSLTSEFKGETCSR 78
           V+   +  ++ R  +     K+S    I+ RRSANYQP IW +++++SL  E+ GETC+R
Sbjct: 28  VTSRTRNGYLARPVQCMVANKVSTSPDIL-RRSANYQPSIWNHDYIESLRIEYVGETCTR 87

Query: 79  RFNELKKKVEVLMRRIVDDPLKQLEFIDILQRLGISYHFENEIKDVLERIYNKSYEDGDR 138
           + N LK++V +++ ++V+ PL+QLE I+ILQRLG+SYHFE EIK +L+ +YN  +  GD 
Sbjct: 88  QINVLKEQVRMMLHKVVN-PLEQLELIEILQRLGLSYHFEEEIKRILDGVYNNDH-GGDT 147

Query: 139 LNKSSLYQTSLEFRLLRQHGFNVSEELISSKK 171
               +LY T+L+FRLLRQHG++VS+E+ +S K
Sbjct: 148 WKAENLYATALKFRLLRQHGYSVSQEVFNSFK 176

[Close]

BLAST of Lsi10G006900 vs. Swiss-Prot
Match: ATESY_VITVI ((-)-alpha-terpineol synthase OS=Vitis vinifera PE=1 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 3.4e-30
Identity = 72/142 (50.70%), Postives = 100/142 (70.42%), Query Frame = 1


Query: 29  PRIAKIKGTTKISEDDQIIVRRSANYQPPIWKYEFVQSLTSEFKGETCSRRFNELKKKVE 88
           PR  K+K     S  ++IIVRR+ANY P IW Y++VQSL S++ GET +RR ++LK+ V+
Sbjct: 31  PRGIKVKIGN--SNCEEIIVRRTANYHPTIWDYDYVQSLRSDYVGETYTRRLDKLKRDVK 90

Query: 89  VLMRRIVDDPLKQLEFIDILQRLGISYHFENEIKDVLERIYNKSYEDGDRLNKSSLYQTS 148
            ++ + V  PL QLE ID+LQRLGI YHF++EIK +L  IYN+ Y   +   K  LY T+
Sbjct: 91  PMLGK-VKKPLDQLELIDVLQRLGIYYHFKDEIKRILNGIYNQ-YNRHEEWQKDDLYATA 150

Query: 149 LEFRLLRQHGFNVSEELISSKK 171
           LEFRLLRQHG++V +++ S  K
Sbjct: 151 LEFRLLRQHGYDVPQDVFSRFK 168

[Close]

BLAST of Lsi10G006900 vs. Swiss-Prot
Match: GTPS_CITLI (Gamma-terpinene synthase, chloroplastic OS=Citrus limon PE=1 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 6.1e-27
Identity = 65/142 (45.77%), Postives = 98/142 (69.01%), Query Frame = 1


Query: 27  FVPRIAKIK-----GTTKISEDDQIIVRRSANYQPPIWKYEFVQSLTSEFKGETCSRRFN 86
           FVP I +++      T+ I   DQ I+RRSA+Y P IW ++++QSL S++KGE+ +R+  
Sbjct: 29  FVPPITQVQYPMAASTSSIKPVDQTIIRRSADYGPTIWSFDYIQSLDSKYKGESYARQLE 88

Query: 87  ELKKKVEVLMR---RIVD-DPLKQLEFIDILQRLGISYHFENEIKDVLERIYNKSYEDGD 146
           +LK++V  +++   ++VD DPL QLE ID L RLG+SYHFE+EIK  L+RI+NK+     
Sbjct: 89  KLKEQVSAMLQQDNKVVDLDPLHQLELIDNLHRLGVSYHFEDEIKRTLDRIHNKN----- 148

Query: 147 RLNKSSLYQTSLEFRLLRQHGF 160
                SLY  +L+FR+LRQ+G+
Sbjct: 149 --TNKSLYARALKFRILRQYGY 163

[Close]

BLAST of Lsi10G006900 vs. Swiss-Prot
Match: RLC1_CITLI ((R)-limonene synthase 1 OS=Citrus limon PE=2 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 1.0e-26
Identity = 63/128 (49.22%), Postives = 90/128 (70.31%), Query Frame = 1


Query: 43  DDQIIVRRSANYQPPIWKYEFVQSLTSEFKGETCSRRFNELKKKVEVLMRRIVDDPLKQL 102
           D+  + RRSANYQP IW ++F+QSL S +  E   RR  EL+ KV++ ++ ++ +PL QL
Sbjct: 47  DNLTVDRRSANYQPSIWDHDFLQSLNSNYTDEAYKRRAEELRGKVKIAIKDVI-EPLDQL 106

Query: 103 EFIDILQRLGISYHFENEIKDVLERIYNKSYEDGDRLNKSSLYQTSLEFRLLRQHGFNVS 162
           E ID LQRLG+++ FE EI+++L  IYN + +   R  K +LY TSLEFRLLRQHG+ VS
Sbjct: 107 ELIDNLQRLGLAHRFETEIRNILNNIYNNNKDYNWR--KENLYATSLEFRLLRQHGYPVS 166

Query: 163 EELISSKK 171
           +E+ +  K
Sbjct: 167 QEVFNGFK 171

[Close]

BLAST of Lsi10G006900 vs. Swiss-Prot
Match: TPS10_RICCO (Terpene synthase 10 OS=Ricinus communis GN=TPS10 PE=2 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 1.8e-26
Identity = 59/122 (48.36%), Postives = 91/122 (74.59%), Query Frame = 1


Query: 44  DQIIVRRSANYQPPIWKYEFVQSLTSEFKGETCSRRFNELKKKVEVLMRRIVDDPLKQLE 103
           ++ I+RRSANY+PPIW ++FVQSL SEF GE   +R ++LK+ V++++ + +  P  Q E
Sbjct: 8   NETIIRRSANYRPPIWDFDFVQSLKSEFVGELNIKRIDKLKEDVKMMLNKTM-APSDQFE 67

Query: 104 FIDILQRLGISYHFENEIKDVLERIYNKSYEDGDRLNKSSLYQTSLEFRLLRQHGFNVSE 163
            ID LQRLG++YHF +EIK +++ IYN S+ + +   K  L+  +L+FRLLRQHG+N+S+
Sbjct: 68  LIDTLQRLGLAYHFGDEIKRIVKSIYN-SHRNDNTWMKEDLHTIALQFRLLRQHGYNISQ 127

Query: 164 EL 166
           E+
Sbjct: 128 EI 127

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BLAST of Lsi10G006900 vs. TrEMBL
Match: A0A0A0LM62_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G299890 PE=3 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 2.0e-45
Identity = 100/169 (59.17%), Postives = 125/169 (73.96%), Query Frame = 1


Query: 1   MALHHVLGSSLVRSFIKQVSFHPKPSFVPRIAK-IKGTTKISEDDQIIVRRSANYQPPIW 60
           MALH    SS    FI++VS +  PSF+PRI K I  TT      Q IVRRS NYQPP W
Sbjct: 1   MALHQFPTSSQTSRFIERVSCNFNPSFIPRIVKVISKTTATRMCSQTIVRRSGNYQPPFW 60

Query: 61  KYEFVQSLTSEFKGETCSRRFNELKKKVEVLMRRIVDDPLKQLEFIDILQRLGISYHFEN 120
           K+EF+QSL SEF GE    RFNELK+++ +++ +I+DDPLKQLE ID LQRLGISYHFEN
Sbjct: 61  KHEFIQSLRSEFGGEIYVGRFNELKREIRLIINQIIDDPLKQLELIDTLQRLGISYHFEN 120

Query: 121 EIKDVLERIYNKSYEDGDRLNKSSLYQTSLEFRLLRQHGFNVSEELISS 169
           EIK+VL+  Y KSYE+ D    ++LY TSLEFRLLRQHGFN+S+++ ++
Sbjct: 121 EIKNVLKTTYEKSYEN-DYWKNNNLYATSLEFRLLRQHGFNLSQDVFNN 168

[Close]

BLAST of Lsi10G006900 vs. TrEMBL
Match: A0A0A0LM58_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G299350 PE=3 SV=1)

HSP 1 Score: 162.2 bits (409), Expect = 5.9e-37
Identity = 78/122 (63.93%), Postives = 100/122 (81.97%), Query Frame = 1


Query: 47  IVRRSANYQPPIWKYEFVQSLTSEFKGETCSRRFNELKKKVEVLMRRIVDDPLKQLEFID 106
           ++RRS NYQP IWK+EF+QSL SEF  E   RRFN+LK ++ V+M  I+DDPLKQLE ID
Sbjct: 6   VIRRSGNYQPSIWKHEFIQSLRSEFAEEIYMRRFNQLKGEIRVIMNTIIDDPLKQLELID 65

Query: 107 ILQRLGISYHFENEIKDVLERIYNKSYEDGDRLNKSSLYQTSLEFRLLRQHGFNVSEELI 166
           +LQRLGISYHFENEIK+VL+ IY+KSYE  +    ++LY TSLEFRLLRQHGFN+S+++ 
Sbjct: 66  MLQRLGISYHFENEIKNVLKTIYDKSYEQ-EHWKNNNLYATSLEFRLLRQHGFNLSQDVF 125

Query: 167 SS 169
           ++
Sbjct: 126 NN 126

[Close]

BLAST of Lsi10G006900 vs. TrEMBL
Match: A0A0A0LMX2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G299920 PE=3 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 3.2e-35
Identity = 88/160 (55.00%), Postives = 110/160 (68.75%), Query Frame = 1


Query: 9   SSLVRSFIKQVSFHPKPSFVPRIAKIKGTTKISEDDQIIVRRSANYQPPIWKYEFVQSLT 68
           SSLV   +   SF+    FVPR+             Q +VRRS NYQP  WKYEF+QSL+
Sbjct: 15  SSLVMERVS--SFNHNSLFVPRVINNVTGGGARMCSQTVVRRSGNYQPSTWKYEFIQSLS 74

Query: 69  SEFKGETCSRRFNELKKKVEVLMRRIV-DDPLKQLEFIDILQRLGISYHFENEIKDVLER 128
           SEF+GE     FNELK +V V+M +I  DDPLKQLEFIDILQRLGISYHFENEIKD+L  
Sbjct: 75  SEFEGEIYVGLFNELKGEVRVIMNKIANDDPLKQLEFIDILQRLGISYHFENEIKDMLTM 134

Query: 129 IYNKSYEDGDRLNKSSLYQTSLEFRLLRQHGFNVSEELIS 168
            Y K++ + D    ++LY TSLEFRLLRQ+GFN+ +++ +
Sbjct: 135 TY-KNHCENDDWKINNLYATSLEFRLLRQYGFNIQQDVFN 171

[Close]

BLAST of Lsi10G006900 vs. TrEMBL
Match: A1JH12_QUEIL (Pinene synthase OS=Quercus ilex GN=pins PE=2 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 1.1e-30
Identity = 71/152 (46.71%), Postives = 108/152 (71.05%), Query Frame = 1


Query: 19  VSFHPKPSFVPRIAKIKGTTKISEDDQIIVRRSANYQPPIWKYEFVQSLTSEFKGETCSR 78
           V+   +  ++ R  +     K+S    I+ RRSANYQP IW +++++SL  EF GETC+R
Sbjct: 28  VTSRTRNGYLARPVQCMVANKVSTSPDIL-RRSANYQPSIWNHDYIESLRIEFVGETCTR 87

Query: 79  RFNELKKKVEVLMRRIVDDPLKQLEFIDILQRLGISYHFENEIKDVLERIYNKSYEDGDR 138
           + N LK++V +++ ++V+ PL+QLE I+ILQRLG+SYHFE EIK +L+ +YN  +  GD 
Sbjct: 88  QINVLKEQVRMMLHKVVN-PLEQLELIEILQRLGLSYHFEEEIKRILDGVYNNDH-GGDT 147

Query: 139 LNKSSLYQTSLEFRLLRQHGFNVSEELISSKK 171
               +LY T+L+FRLLRQHG++VS+E+ +S K
Sbjct: 148 WKAENLYATALKFRLLRQHGYSVSQEVFNSFK 176

[Close]

BLAST of Lsi10G006900 vs. TrEMBL
Match: W9QVA8_9ROSA (Myrcene synthase OS=Morus notabilis GN=L484_016581 PE=3 SV=1)

HSP 1 Score: 141.0 bits (354), Expect = 1.4e-30
Identity = 69/131 (52.67%), Postives = 96/131 (73.28%), Query Frame = 1


Query: 38  TKISEDDQIIVRRSANYQPPIWKYEFVQSLTSEFKGETCSRRFNELKKKVEVLMRRIVDD 97
           T+IS D++II RRSANYQPPIW ++FVQSL +++  E+ + RF  LK+ V  ++  + DD
Sbjct: 50  TRISNDEKIIARRSANYQPPIWNFDFVQSLQTQYTRESYAERFENLKEVVREILSNLEDD 109

Query: 98  PLKQLEFIDILQRLGISYHFENEIKDVLERIYNKSYEDGDRLNKSSLYQTSLEFRLLRQH 157
           PL QLE ID+LQRLGISYHF+ EIK VLE IYN + +       + LY  +LEFRLLRQH
Sbjct: 110 PLAQLEHIDMLQRLGISYHFDEEIKCVLEEIYNNNSK-----KNNDLYAIALEFRLLRQH 169

Query: 158 GFNVSEELISS 169
           G+++ +E+ ++
Sbjct: 170 GYSMPQEVFNA 175

[Close]

BLAST of Lsi10G006900 vs. TAIR10
Match: AT4G16740.1 (AT4G16740.1 terpene synthase 03)

HSP 1 Score: 100.5 bits (249), Expect = 1.1e-21
Identity = 54/124 (43.55%), Postives = 80/124 (64.52%), Query Frame = 1


Query: 46  IIVRRSANYQPPIWKYEFVQSLTSEFKGETCSRRFNELKKKVEVLMRRIVDDPLKQLEFI 105
           ++ RRSANYQP +W++E++ SL + +  E    R   LK++V  ++    +  L+QLE I
Sbjct: 23  LVSRRSANYQPSLWQHEYLLSLGNTYVKEDNVERVTLLKQEVSKMLNE-TEGLLEQLELI 82

Query: 106 DILQRLGISYHFENEIKDVLERIYNKSYEDG----DRLNKSSLYQTSLEFRLLRQHGFNV 165
           D LQRLG+SYHFE EIK  L  ++ K+        DR     LY T+LEFRLLRQHGF++
Sbjct: 83  DTLQRLGVSYHFEQEIKKTLTNVHVKNVRAHKNRIDRNRWGDLYATALEFRLLRQHGFSI 142

[Close]

BLAST of Lsi10G006900 vs. TAIR10
Match: AT2G24210.1 (AT2G24210.1 terpene synthase 10)

HSP 1 Score: 100.1 bits (248), Expect = 1.4e-21
Identity = 55/119 (46.22%), Postives = 76/119 (63.87%), Query Frame = 1


Query: 47  IVRRSANYQPPIWKYEFVQSLTSEFKGETCSRRFNELKKKVEVLMRRIVDDPLKQLEFID 106
           + RRSANYQP  W +  + S+ ++F  +   R  + LK+KV  ++       L QLEFID
Sbjct: 44  VQRRSANYQPSRWDHHHLLSVENKFAKDKRVRERDLLKEKVRKMLNDEQKTYLDQLEFID 103

Query: 107 ILQRLGISYHFENEIKDVLERIYNKSYEDGDRLNKSSLYQTSLEFRLLRQHGFNVSEEL 166
            LQ+LG+SYHFE EI ++L   Y K   D   + +S L+ T+LEFRL RQHGFNVSE++
Sbjct: 104 DLQKLGVSYHFEAEIDNILTSSYKK---DRTNIQESDLHATALEFRLFRQHGFNVSEDV 159

[Close]

BLAST of Lsi10G006900 vs. TAIR10
Match: AT3G25830.1 (AT3G25830.1 terpene synthase-like sequence-1,8-cineole)

HSP 1 Score: 97.1 bits (240), Expect = 1.2e-20
Identity = 58/134 (43.28%), Postives = 83/134 (61.94%), Query Frame = 1


Query: 35  KGTTKISEDDQIIV--RRSANYQPPIWKYEFVQSLTSEFKGETCSRRFNELKKKVEVLMR 94
           K  TK + D  ++   RRS NYQP  W + ++ S+ +++  ET     + LKKKV+ ++ 
Sbjct: 31  KSMTKTTPDTTLVELSRRSGNYQPSPWNHCYLLSIENKYASETEVITRDVLKKKVKSMLD 90

Query: 95  RIVDDPLKQLEFIDILQRLGISYHFENEIKDVLERIYNKSYEDGDRLNK-SSLYQTSLEF 154
                 L+QLE ID LQ+LG+SYHFE EI D L  ++ K   +  + +K   L+ TSLEF
Sbjct: 91  DEKKSRLEQLELIDDLQKLGVSYHFEIEINDTLTDLHLKMGRNCWKCDKEEDLHATSLEF 150

Query: 155 RLLRQHGFNVSEEL 166
           RLLRQHGF+VSE +
Sbjct: 151 RLLRQHGFDVSENI 164

[Close]

BLAST of Lsi10G006900 vs. TAIR10
Match: AT3G25820.1 (AT3G25820.1 terpene synthase-like sequence-1,8-cineole)

HSP 1 Score: 97.1 bits (240), Expect = 1.2e-20
Identity = 58/134 (43.28%), Postives = 83/134 (61.94%), Query Frame = 1


Query: 35  KGTTKISEDDQIIV--RRSANYQPPIWKYEFVQSLTSEFKGETCSRRFNELKKKVEVLMR 94
           K  TK + D  ++   RRS NYQP  W + ++ S+ +++  ET     + LKKKV+ ++ 
Sbjct: 31  KSMTKTTPDTTLVELSRRSGNYQPSPWNHCYLLSIENKYASETEVITRDVLKKKVKSMLD 90

Query: 95  RIVDDPLKQLEFIDILQRLGISYHFENEIKDVLERIYNKSYEDGDRLNK-SSLYQTSLEF 154
                 L+QLE ID LQ+LG+SYHFE EI D L  ++ K   +  + +K   L+ TSLEF
Sbjct: 91  DEKKSRLEQLELIDDLQKLGVSYHFEIEINDTLTDLHLKMGRNCWKCDKEEDLHATSLEF 150

Query: 155 RLLRQHGFNVSEEL 166
           RLLRQHGF+VSE +
Sbjct: 151 RLLRQHGFDVSENI 164

[Close]

BLAST of Lsi10G006900 vs. TAIR10
Match: AT3G25810.1 (AT3G25810.1 Terpenoid cyclases/Protein prenyltransferases superfamily protein)

HSP 1 Score: 90.1 bits (222), Expect = 1.4e-18
Identity = 49/119 (41.18%), Postives = 78/119 (65.55%), Query Frame = 1


Query: 49  RRSANYQPPIWKYEFVQSLTSEFKGETCSRRFNELKKKVEVLMRRI-VDDPLKQLEFIDI 108
           RRS NYQP  W + ++ S+ +++  E      + LKKKV+ ++  +     L++LE ID 
Sbjct: 47  RRSGNYQPSSWDHSYLLSIENKYVNEKEVITRHVLKKKVKKMLEEVETKSRLEKLELIDD 106

Query: 109 LQRLGISYHFENEIKDVLERIYNKSYEDGDRLNK-SSLYQTSLEFRLLRQHGFNVSEEL 166
           LQ+LG+SYHFE EI ++L   + ++ ++  + +K   L+ T+LEFRLLRQHGF VSE++
Sbjct: 107 LQKLGVSYHFEQEINNILTNFHLENGKNIWKCDKEEDLHATALEFRLLRQHGFGVSEDI 165

[Close]

BLAST of Lsi10G006900 vs. NCBI nr
Match: gi|700207000|gb|KGN62119.1| (hypothetical protein Csa_2G299890 [Cucumis sativus])

HSP 1 Score: 190.3 bits (482), Expect = 2.9e-45
Identity = 100/169 (59.17%), Postives = 125/169 (73.96%), Query Frame = 1


Query: 1   MALHHVLGSSLVRSFIKQVSFHPKPSFVPRIAK-IKGTTKISEDDQIIVRRSANYQPPIW 60
           MALH    SS    FI++VS +  PSF+PRI K I  TT      Q IVRRS NYQPP W
Sbjct: 1   MALHQFPTSSQTSRFIERVSCNFNPSFIPRIVKVISKTTATRMCSQTIVRRSGNYQPPFW 60

Query: 61  KYEFVQSLTSEFKGETCSRRFNELKKKVEVLMRRIVDDPLKQLEFIDILQRLGISYHFEN 120
           K+EF+QSL SEF GE    RFNELK+++ +++ +I+DDPLKQLE ID LQRLGISYHFEN
Sbjct: 61  KHEFIQSLRSEFGGEIYVGRFNELKREIRLIINQIIDDPLKQLELIDTLQRLGISYHFEN 120

Query: 121 EIKDVLERIYNKSYEDGDRLNKSSLYQTSLEFRLLRQHGFNVSEELISS 169
           EIK+VL+  Y KSYE+ D    ++LY TSLEFRLLRQHGFN+S+++ ++
Sbjct: 121 EIKNVLKTTYEKSYEN-DYWKNNNLYATSLEFRLLRQHGFNLSQDVFNN 168

[Close]

BLAST of Lsi10G006900 vs. NCBI nr
Match: gi|659122221|ref|XP_008461028.1| (PREDICTED: terpene synthase 10-like [Cucumis melo])

HSP 1 Score: 183.0 bits (463), Expect = 4.6e-43
Identity = 94/168 (55.95%), Postives = 122/168 (72.62%), Query Frame = 1


Query: 1   MALHHVLGSSLVRSFIKQVSFHPKPSFVPRIAKIKGTTKISEDDQIIVRRSANYQPPIWK 60
           MALH    SS    F ++VS +  PSF+PRI K    T      + IVRRS NYQPPIWK
Sbjct: 1   MALHQFPTSSQTSRFFERVSCNFNPSFMPRIVKAISVTTRIMCSRTIVRRSGNYQPPIWK 60

Query: 61  YEFVQSLTSEFKGETCSRRFNELKKKVEVLMRRIVDDPLKQLEFIDILQRLGISYHFENE 120
           +EF+QSL SEF  E    RFNELK+++ +++ +I+DDPL+QLE +D LQRLGISYHFENE
Sbjct: 61  HEFIQSLRSEFAEEIYVGRFNELKREIRLIVNQIIDDPLRQLELVDTLQRLGISYHFENE 120

Query: 121 IKDVLERIYNKSYEDGDRLNKSSLYQTSLEFRLLRQHGFNVSEELISS 169
           IK+VL+  + KSYE+ D   K +LY TSLEFRLLRQHGFN+S+++ ++
Sbjct: 121 IKNVLKTAFEKSYEN-DYWKKKNLYATSLEFRLLRQHGFNLSQDVFNN 167

[Close]

BLAST of Lsi10G006900 vs. NCBI nr
Match: gi|659122225|ref|XP_008461030.1| (PREDICTED: terpene synthase 10-like [Cucumis melo])

HSP 1 Score: 171.0 bits (432), Expect = 1.8e-39
Identity = 91/169 (53.85%), Postives = 122/169 (72.19%), Query Frame = 1


Query: 1   MALHHVLGSSLVRSFIKQVSFHPKPS--FVPRIAKIKGTTKISEDDQIIVRRSANYQPPI 60
           MALH +  +  +  FI+++SF+       VPR  K+ G   +   ++ IVRRS NY+PPI
Sbjct: 1   MALHQLFATFSISPFIQRISFNFDHLCVHVPRTFKVIGAATMC--NKTIVRRSGNYRPPI 60

Query: 61  WKYEFVQSLTSEFKGETCSRRFNELKKKVEVLMRRIVDDPLKQLEFIDILQRLGISYHFE 120
           WK+EF+QSL SEF+ E    RFNELK ++ V+M  I+DDPLKQLE ID+LQRLGISYHFE
Sbjct: 61  WKHEFIQSLRSEFEEEIYIGRFNELKGEIRVIMNTIIDDPLKQLELIDMLQRLGISYHFE 120

Query: 121 NEIKDVLERIYNKSYEDGDRLNKSSLYQTSLEFRLLRQHGFNVSEELIS 168
           NEIK+VL+  Y+ SYE  +    ++LY TSLEFRLLRQHGFN+S+++ +
Sbjct: 121 NEIKNVLKTTYDTSYEK-EHWKNNNLYATSLEFRLLRQHGFNLSQDVFN 166

[Close]

BLAST of Lsi10G006900 vs. NCBI nr
Match: gi|659122004|ref|XP_008460926.1| (PREDICTED: terpene synthase 10-like [Cucumis melo])

HSP 1 Score: 167.9 bits (424), Expect = 1.5e-38
Identity = 95/170 (55.88%), Postives = 117/170 (68.82%), Query Frame = 1


Query: 1   MALHHVLGSSLVRSFIKQ--VSFHPKPSFVPRIAKIKGTTKISEDDQIIVRRSANYQPPI 60
           MALH +  +S   S + +   SF+    FVPR+     T+      Q IVRRS NYQPP 
Sbjct: 1   MALHQIYFTSFPASSVVERVYSFNHNSLFVPRVMN-NVTSGARMCSQTIVRRSGNYQPPT 60

Query: 61  WKYEFVQSLTSEFKGETCSRRFNELKKKVEVLMRRIV-DDPLKQLEFIDILQRLGISYHF 120
           WK+EF+QSL+SEF GE    RFNELK +V V+M +I  DDPLKQLEFIDILQRLGISYHF
Sbjct: 61  WKHEFIQSLSSEFAGEIYVGRFNELKGEVRVVMNKIANDDPLKQLEFIDILQRLGISYHF 120

Query: 121 ENEIKDVLERIYNKSYEDGDRLNKSSLYQTSLEFRLLRQHGFNVSEELIS 168
           ENEIKD+L   Y K  E+ D    ++LY TSLEFRLLRQHGFN+ +++ +
Sbjct: 121 ENEIKDMLTTTYKKCCENDD-WKCNNLYATSLEFRLLRQHGFNIPQDIFN 168

[Close]

BLAST of Lsi10G006900 vs. NCBI nr
Match: gi|778670174|ref|XP_011649391.1| (PREDICTED: terpene synthase 10 [Cucumis sativus])

HSP 1 Score: 164.5 bits (415), Expect = 1.7e-37
Identity = 80/124 (64.52%), Postives = 101/124 (81.45%), Query Frame = 1


Query: 45  QIIVRRSANYQPPIWKYEFVQSLTSEFKGETCSRRFNELKKKVEVLMRRIVDDPLKQLEF 104
           Q IVRRS NYQPP WK+EF+QSL SEF GE    RFNELK+++ +++ +I+DDPLKQLE 
Sbjct: 4   QTIVRRSGNYQPPFWKHEFIQSLRSEFGGEIYVGRFNELKREIRLIINQIIDDPLKQLEL 63

Query: 105 IDILQRLGISYHFENEIKDVLERIYNKSYEDGDRLNKSSLYQTSLEFRLLRQHGFNVSEE 164
           ID LQRLGISYHFENEIK+VL+  Y KSYE+ D    ++LY TSLEFRLLRQHGFN+S++
Sbjct: 64  IDTLQRLGISYHFENEIKNVLKTTYEKSYEN-DYWKNNNLYATSLEFRLLRQHGFNLSQD 123

Query: 165 LISS 169
           + ++
Sbjct: 124 VFNN 126

The following BLAST results are available for this feature:

BLAST of Lsi10G006900 vs. Swiss-Prot


Match Name	E-value	Identity	Description
MYRS_QUEIL	2.2e-32	46.05	Myrcene synthase, chloroplastic OS=Quercus ilex PE=1 SV=1	[more]
ATESY_VITVI	3.4e-30	50.70	(-)-alpha-terpineol synthase OS=Vitis vinifera PE=1 SV=1	[more]
GTPS_CITLI	6.1e-27	45.77	Gamma-terpinene synthase, chloroplastic OS=Citrus limon PE=1 SV=1	[more]
RLC1_CITLI	1.0e-26	49.22	(R)-limonene synthase 1 OS=Citrus limon PE=2 SV=1	[more]
TPS10_RICCO	1.8e-26	48.36	Terpene synthase 10 OS=Ricinus communis GN=TPS10 PE=2 SV=1	[more]

BLAST of Lsi10G006900 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LM62_CUCSA	2.0e-45	59.17	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G299890 PE=3 SV=1	[more]
A0A0A0LM58_CUCSA	5.9e-37	63.93	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G299350 PE=3 SV=1	[more]
A0A0A0LMX2_CUCSA	3.2e-35	55.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G299920 PE=3 SV=1	[more]
A1JH12_QUEIL	1.1e-30	46.71	Pinene synthase OS=Quercus ilex GN=pins PE=2 SV=1	[more]
W9QVA8_9ROSA	1.4e-30	52.67	Myrcene synthase OS=Morus notabilis GN=L484_016581 PE=3 SV=1	[more]

BLAST of Lsi10G006900 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G16740.1	1.1e-21	43.55	terpene synthase 03	[more]
AT2G24210.1	1.4e-21	46.22	terpene synthase 10	[more]
AT3G25830.1	1.2e-20	43.28	terpene synthase-like sequence-1,8-cineole	[more]
AT3G25820.1	1.2e-20	43.28	terpene synthase-like sequence-1,8-cineole	[more]
AT3G25810.1	1.4e-18	41.18	Terpenoid cyclases/Protein prenyltransferases superfamily protein	[more]

BLAST of Lsi10G006900 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700207000\|gb\|KGN62119.1\|	2.9e-45	59.17	hypothetical protein Csa_2G299890 [Cucumis sativus]	[more]
gi\|659122221\|ref\|XP_008461028.1\|	4.6e-43	55.95	PREDICTED: terpene synthase 10-like [Cucumis melo]	[more]
gi\|659122225\|ref\|XP_008461030.1\|	1.8e-39	53.85	PREDICTED: terpene synthase 10-like [Cucumis melo]	[more]
gi\|659122004\|ref\|XP_008460926.1\|	1.5e-38	55.88	PREDICTED: terpene synthase 10-like [Cucumis melo]	[more]
gi\|778670174\|ref\|XP_011649391.1\|	1.7e-37	64.52	PREDICTED: terpene synthase 10 [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0016829	lyase activity
GO:0010333	terpene synthase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0008152	metabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR008930	Terpenoid_cyclase/PrenylTrfase
IPR001906	Terpene_synth_N

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008152	metabolic process
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0010333	terpene synthase activity
molecular_function	GO:0000287	magnesium ion binding
molecular_function	GO:0016829	lyase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi10G006900.1	Lsi10G006900.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001906	Terpene synthase, N-terminal domain	GENE3D	G3DSA:1.50.30.10		coord: 76..166 score: 1.9
IPR001906	Terpene synthase, N-terminal domain	PFAM	PF01397	Terpene_synth	coord: 58..166 score: 5.2
IPR008930	Terpenoid cyclases/protein prenyltransferase alpha-alpha toroid	unknown	SSF48239	Terpenoid cyclases/Protein prenyltransferases	coord: 55..166 score: 7.53
None	No IPR available	PANTHER	PTHR31225	FAMILY NOT NAMED	coord: 43..168 score: 2.4
None	No IPR available	PANTHER	PTHR31225:SF16	1,8-CINEOLE SYNTHASE 1, CHLOROPLASTIC-RELATED	coord: 43..168 score: 2.4

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi10G006900	CmoCh20G011080	Cucurbita moschata (Rifu)	cmolsiB492

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi10G006900	Wild cucumber (PI 183967)	cpilsiB091
Lsi10G006900	Cucumber (Chinese Long) v2	culsiB093
Lsi10G006900	Melon (DHL92) v3.5.1	lsimeB056
Lsi10G006900	Watermelon (Charleston Gray)	lsiwcgB056
Lsi10G006900	Watermelon (97103) v1	lsiwmB060
Lsi10G006900	Watermelon (97103) v1	lsiwmB066
Lsi10G006900	Cucumber (Gy14) v2	cgyblsiB086
Lsi10G006900	Cucumber (Gy14) v2	cgyblsiB385
Lsi10G006900	Melon (DHL92) v3.6.1	lsimedB058
Lsi10G006900	Silver-seed gourd	carlsiB213
Lsi10G006900	Silver-seed gourd	carlsiB962
Lsi10G006900	Silver-seed gourd	carlsiB967
Lsi10G006900	Cucumber (Chinese Long) v3	cuclsiB106
Lsi10G006900	Cucumber (Chinese Long) v3	cuclsiB447
Lsi10G006900	Watermelon (97103) v2	lsiwmbB037
Lsi10G006900	Watermelon (97103) v2	lsiwmbB053
Lsi10G006900	Wax gourd	lsiwgoB072
Lsi10G006900	Wax gourd	lsiwgoB083
Lsi10G006900	Wax gourd	lsiwgoB091
Lsi10G006900	Bottle gourd (USVL1VR-Ls)	lsilsiB029
Lsi10G006900	Cucumber (Gy14) v1	cgylsiB409
Lsi10G006900	Cucurbita maxima (Rimu)	cmalsiB508
Lsi10G006900	Cucurbita maxima (Rimu)	cmalsiB551
Lsi10G006900	Cucurbita maxima (Rimu)	cmalsiB592
Lsi10G006900	Cucurbita moschata (Rifu)	cmolsiB527
Lsi10G006900	Cucurbita moschata (Rifu)	cmolsiB536
Lsi10G006900	Cucurbita moschata (Rifu)	cmolsiB577
Lsi10G006900	Cucurbita pepo (Zucchini)	cpelsiB222
Lsi10G006900	Cucurbita pepo (Zucchini)	cpelsiB593