Lsi10G004590 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi10G004590
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Aquaporin-4
Location	chr10 : 6669279 .. 6671609 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTCCACTGCAGCTCAGGAGGATCTTGCTTTATCAATGGAAGAAGAAAGAGGCTTTGATGAACAGAATTTTACTCAAGAAAGAGGCTTTGATGAACAGAATTTTACTCAAACAGATTCTTCCTCTTTTCTCTCAAGATTTCTTCTTTACATTGGTGCTCATGAACTTTTCTCACAAGAGGTTTGATTCAATTCCAATTATATGTATTTTATGTCAACTTTTTTTTTTTTACCTTAATTTCTTTCTTTTAATACCCTTCCTCTGAAAAAGGTGTTTTTTTTTTCTTCGAGTATATCGTTATAAGTTATGTTTAATAGGTTGATGAACTAATTTTAAGAAAAAAATGGCGGTTAAAAGTCATTTTTGGTCCATGAATTTTTGTAGAAGTTAACAGTTTAGTTTATAAATTTTGATTTGTAACGATTTAGTCTCTATACTTTCAATTTTGTAACTATTTAGTCCCTTAACTTTAGTACGTTACGTAGTAATTTTGTCTCGATACTTTAAAATATGTAACAATTTAGTTTGAAAATATTTAATGGAAGAGTAATACTTGAGTCCATTTCAAATGCACCTAATTTTGTCTCACAATTTATTCACACACACAAAAAAAAGTTTAATCCTTTTTGAAAAGAAGTAAGGAGAGTTCGTCATGTGACAAACTATGATTTGATAATTTGATGGGATATCATGTGGCAAAAAAACCACAAACATAGGTGTAGTCTAGGATGCACCTAAGTATTTTTCTTATATAAGTAGATTAATAAACTAATTAGGGATTTAATATGTTTTATAAACTATAAAGTATAAATCGATAAGTAATAAATATCTACATTTAATTTTGATGAACTTTACGAAAAGGATCAAATTATTACAAATTTGGAAGTATAGAGATTAGATAGTTATATACTAAAGTGAAAAGACTAATGTTACGAAATTGAAAATACAATGACTAAATCGTTACAAATCAAAGTTTAGGGATTAAATTGTTACAAGGTTAAAGGCCCAAATACTATTTTTGTCAAAAAAAAAAAAAAAAAAAATTATAAACTTTGAAATTTGTGTATTTTATATATATCTGAACTTTCAAATTTTTAACAAAAGGTATTTTTACCTTTAAAATATGTCCTAAAATATTTTGTGACAATCAAGGTAATATTATTGCAATTTTTAAAATAATAATTAATAAGGATATTTTTTTATCCAATTTACATAGTTGATATATATATATATTTATATATATATATTTTAGATAAATTGCAAAAACTAAAACTACCCTTAAAGTGTGATTGTTGCAATTACACCTTTAAATTTGTAAAAATGAAATCCTCAAACTCAAACTTATGCAAATGTTAAAATTGGACTCCCAAATTTATATAATTATAGAAATTGTAATAATTTAAGAATATAATTACAACTACGATGGTAATTCATTTATTCAATTCGCCCTTTTTTTAATTATCATTTAACTAAGAAAATTGATGGATTTTTCTTTTTGAAACACAGATGTGGAAAGCAGCGCTGACAGAATTAGTAGCAACAGCGTTTCTGATATTTTGCTTAACAAGTTCCATAATCTCGTGCTTGAACTCACACGAATCAGATCCAAAGCTCCTAATTCCGATCGCCGTTTTCATCATCCTCTTCCTCTTCCTCCTCGTCACTTTCCCTCTCTCCGGCGGCTTCCTGAGCCCCATCTTCGCTTTCATCGCCGCCCTCCGCGGCGTCATAACCTTCACACGCGCCGCCATCTACATTCTTGGCCAATGTCTCGGCTCCATTATCGCCTTTCTCATGATCAAGGATGCAATGAGCCCCGATGTTGCTGACAAATATTCTCTCGGCGGCTGCACCATCCGCGGCACCGGCGACACTCCCGGAATCGGCCTCACCACCGCTCTCGTCCTCGAATTCGCCTGCACCTTCGTCGTTCTCTATATCGGAGTCACGGTGGTGCTCGATGAGAAGATGAGCGAGCGGCTTGGTTTGCCAATGGTGTGTGGGATGATTGCGGGGAGTTCTGCGGTGGCGGTTTTCGTGTCGACGACTATCACAGGGCGGGCCGGATACGGCGGGGTGGGGCTGAATCCGGCGAGGTGTTTAGGGCCGGCGTTGTTGAGAGGGGGGCGGTTGTGGGAAGGGCATTGGGTGTTCTGGGTGGGGCCGTTTGTGGCGTGTATGGTTTATTATGGATTTTCAGTGAATTTGCCAAAGGGGCAATTGGTTGTAGAGAAGGGGGAGATAGGGATTTTGAAGATGGCCGGAGGTTGTTGGCGGCAACGGCGCCGAAAGTTGGAGGGAAAATTATGTCAAAATGTTGATATCTGA

mRNA sequence

ATGGCTTCCACTGCAGCTCAGGAGGATCTTGCTTTATCAATGGAAGAAGAAAGAGGCTTTGATGAACAGAATTTTACTCAAGAAAGAGGCTTTGATGAACAGAATTTTACTCAAACAGATTCTTCCTCTTTTCTCTCAAGATTTCTTCTTTACATTGGTGCTCATGAACTTTTCTCACAAGAGATGTGGAAAGCAGCGCTGACAGAATTAGTAGCAACAGCGTTTCTGATATTTTGCTTAACAAGTTCCATAATCTCGTGCTTGAACTCACACGAATCAGATCCAAAGCTCCTAATTCCGATCGCCGTTTTCATCATCCTCTTCCTCTTCCTCCTCGTCACTTTCCCTCTCTCCGGCGGCTTCCTGAGCCCCATCTTCGCTTTCATCGCCGCCCTCCGCGGCGTCATAACCTTCACACGCGCCGCCATCTACATTCTTGGCCAATGTCTCGGCTCCATTATCGCCTTTCTCATGATCAAGGATGCAATGAGCCCCGATGTTGCTGACAAATATTCTCTCGGCGGCTGCACCATCCGCGGCACCGGCGACACTCCCGGAATCGGCCTCACCACCGCTCTCGTCCTCGAATTCGCCTGCACCTTCGTCGTTCTCTATATCGGAGTCACGGTGGTGCTCGATGAGAAGATGAGCGAGCGGCTTGGTTTGCCAATGGTGTGTGGGATGATTGCGGGGAGTTCTGCGGTGGCGGTTTTCGTGTCGACGACTATCACAGGGCGGGCCGGATACGGCGGGGTGGGGCTGAATCCGGCGAGGTGTTTAGGGCCGGCGTTGTTGAGAGGGGGGCGGTTGTGGGAAGGGCATTGGGTGTTCTGGGTGGGGCCGTTTGTGGCGTGTATGGTTTATTATGGATTTTCAGTGAATTTGCCAAAGGGGCAATTGGTTGTAGAGAAGGGGGAGATAGGGATTTTGAAGATGGCCGGAGGTTGTTGGCGGCAACGGCGCCGAAAGTTGGAGGGAAAATTATGTCAAAATGTTGATATCTGA

Coding sequence (CDS)

ATGGCTTCCACTGCAGCTCAGGAGGATCTTGCTTTATCAATGGAAGAAGAAAGAGGCTTTGATGAACAGAATTTTACTCAAGAAAGAGGCTTTGATGAACAGAATTTTACTCAAACAGATTCTTCCTCTTTTCTCTCAAGATTTCTTCTTTACATTGGTGCTCATGAACTTTTCTCACAAGAGATGTGGAAAGCAGCGCTGACAGAATTAGTAGCAACAGCGTTTCTGATATTTTGCTTAACAAGTTCCATAATCTCGTGCTTGAACTCACACGAATCAGATCCAAAGCTCCTAATTCCGATCGCCGTTTTCATCATCCTCTTCCTCTTCCTCCTCGTCACTTTCCCTCTCTCCGGCGGCTTCCTGAGCCCCATCTTCGCTTTCATCGCCGCCCTCCGCGGCGTCATAACCTTCACACGCGCCGCCATCTACATTCTTGGCCAATGTCTCGGCTCCATTATCGCCTTTCTCATGATCAAGGATGCAATGAGCCCCGATGTTGCTGACAAATATTCTCTCGGCGGCTGCACCATCCGCGGCACCGGCGACACTCCCGGAATCGGCCTCACCACCGCTCTCGTCCTCGAATTCGCCTGCACCTTCGTCGTTCTCTATATCGGAGTCACGGTGGTGCTCGATGAGAAGATGAGCGAGCGGCTTGGTTTGCCAATGGTGTGTGGGATGATTGCGGGGAGTTCTGCGGTGGCGGTTTTCGTGTCGACGACTATCACAGGGCGGGCCGGATACGGCGGGGTGGGGCTGAATCCGGCGAGGTGTTTAGGGCCGGCGTTGTTGAGAGGGGGGCGGTTGTGGGAAGGGCATTGGGTGTTCTGGGTGGGGCCGTTTGTGGCGTGTATGGTTTATTATGGATTTTCAGTGAATTTGCCAAAGGGGCAATTGGTTGTAGAGAAGGGGGAGATAGGGATTTTGAAGATGGCCGGAGGTTGTTGGCGGCAACGGCGCCGAAAGTTGGAGGGAAAATTATGTCAAAATGTTGATATCTGA

Protein sequence

MASTAAQEDLALSMEEERGFDEQNFTQERGFDEQNFTQTDSSSFLSRFLLYIGAHELFSQEMWKAALTELVATAFLIFCLTSSIISCLNSHESDPKLLIPIAVFIILFLFLLVTFPLSGGFLSPIFAFIAALRGVITFTRAAIYILGQCLGSIIAFLMIKDAMSPDVADKYSLGGCTIRGTGDTPGIGLTTALVLEFACTFVVLYIGVTVVLDEKMSERLGLPMVCGMIAGSSAVAVFVSTTITGRAGYGGVGLNPARCLGPALLRGGRLWEGHWVFWVGPFVACMVYYGFSVNLPKGQLVVEKGEIGILKMAGGCWRQRRRKLEGKLCQNVDI

Homology

[Close]

BLAST of Lsi10G004590 vs. Swiss-Prot
Match: TIP12_MAIZE (Aquaporin TIP1-2 OS=Zea mays GN=TIP1-2 PE=2 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 4.1e-16
Identity = 74/244 (30.33%), Postives = 121/244 (49.59%), Query Frame = 1


Query: 56  ELFSQEMWKAALTELVATAFLIFCLTSSIIS---CLNSHESDPKLLIPIAVFIILFLFLL 115
           EL   +  KAA+ E ++T   +F  + S ++     +   + P  LI  ++   L LF+ 
Sbjct: 13  ELSHPDTAKAAVAEFISTLIFVFAGSGSGMAFSKLTDGGAATPAGLIAASLAHALALFVA 72

Query: 116 VTFP--LSGGFLSPIFAFIAALRGVITFTRAAIYILGQCLGSIIAFLMIKDAMSPDVADK 175
           V+    +SGG ++P   F A + G I+  +A +Y + Q LGS++A L++K A        
Sbjct: 73  VSVGANISGGHVNPAVTFGAFVGGNISLLKALVYWVAQLLGSVVACLLLKIAT------- 132

Query: 176 YSLGGCTIRGTGDTPGIGLTTALVLEFACTFVVLYIGVTVVLDEKMSERLGL--PMVCGM 235
              GG  +     + G+G   A+VLE   TF ++Y      +D K  + LG+  P+  G 
Sbjct: 133 ---GGAALGAFSLSAGVGAMNAVVLEMVMTFGLVYTVYATAVDPKKGD-LGVIAPIAIGF 192

Query: 236 IAGSSAVAVFVSTTITGRAGYGGVGLNPARCLGPALLRGGRLWEGHWVFWVGPF----VA 289
           I G++ +A            + G  +NPA   GPA++ G  +WE HWV+WVGP     +A
Sbjct: 193 IVGANILA---------GGAFDGASMNPAVSFGPAVVTG--VWENHWVYWVGPLAGAAIA 234

[Close]

BLAST of Lsi10G004590 vs. Swiss-Prot
Match: TIP12_ORYSJ (Probable aquaporin TIP1-2 OS=Oryza sativa subsp. japonica GN=TIP1-2 PE=2 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 2.1e-15
Identity = 69/239 (28.87%), Postives = 117/239 (48.95%), Query Frame = 1


Query: 56  ELFSQEMWKAALTELVATAFLIFCLTSSIIS---CLNSHESDPKLLIP--IAVFIILFLF 115
           EL   +  KAA+ E ++    +F  + S ++     +   + P  LI   +A  + LF+ 
Sbjct: 13  ELSHPDTAKAAVAEFISMLIFVFAGSGSGMAFSKLTDGGGTTPSGLIAASLAHALALFVA 72

Query: 116 LLVTFPLSGGFLSPIFAFIAALRGVITFTRAAIYILGQCLGSIIAFLMIKDAMSPDVADK 175
           + V   +SGG ++P   F A + G I+  +A +Y + Q LGS++A L++K A        
Sbjct: 73  VAVGANISGGHVNPAVTFGAFVGGNISLVKAVVYWVAQLLGSVVACLLLKIAT------- 132

Query: 176 YSLGGCTIRGTGDTPGIGLTTALVLEFACTFVVLYIGVTVVLDEKMSERLGL--PMVCGM 235
              GG  +     + G+G   A+V E   TF ++Y      +D K  + LG+  P+  G 
Sbjct: 133 ---GGAAVGAFSLSAGVGAWNAVVFEIVMTFGLVYTVYATAVDPKKGD-LGVIAPIAIGF 192

Query: 236 IAGSSAVAVFVSTTITGRAGYGGVGLNPARCLGPALLRGGRLWEGHWVFWVGPFVACMV 288
           I G++ +A            + G  +NPA   GPA++ G  +W+ HWV+W+GPFV   +
Sbjct: 193 IVGANILA---------GGAFDGASMNPAVSFGPAVVTG--VWDNHWVYWLGPFVGAAI 229

[Close]

BLAST of Lsi10G004590 vs. Swiss-Prot
Match: PIP21_ORYSJ (Probable aquaporin PIP2-1 OS=Oryza sativa subsp. japonica GN=PIP2-1 PE=2 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 2.1e-15
Identity = 70/251 (27.89%), Postives = 119/251 (47.41%), Query Frame = 1


Query: 52  IGAHELFSQEMWKAALTELVATAFLIFCLTSSIISCLNSHESDPK-------------LL 111
           I A EL S  +++A + E +AT   ++   +++I     H++D               L 
Sbjct: 30  IDAAELGSWSLYRAVIAEFIATLLFLYITVATVIGY--KHQTDASASGADAACGGVGVLG 89

Query: 112 IPIAVFIILFLFLLVTFPLSGGFLSPIFAFIAALRGVITFTRAAIYILGQCLGSIIAFLM 171
           I  A   ++F+ +  T  +SGG ++P   F   L   ++  RA +YI+ QCLG+I    +
Sbjct: 90  IAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLARKVSLVRAILYIVAQCLGAICGVGL 149

Query: 172 IKDAMSPDVADKYSLGGCTIRGTGDTPGIGLTTALVLEFACTFVVLYIGVTVVLDEKMSE 231
           +K A      ++Y  GG      G + G GL      E   TFV++Y   +    ++ + 
Sbjct: 150 VK-AFQSAYFNRYG-GGANTLAAGYSKGTGLAA----EIIGTFVLVYTVFSATDPKRNAR 209

Query: 232 RLGLPMVCGMIAGSSAVAVFVST-TITGRAGYGGVGLNPARCLGPALL-RGGRLWEGHWV 288
              +P++  +  G +   V ++T  IT      G G+NPAR +G A++    + W  HW+
Sbjct: 210 DSHVPVLAPLPIGFAVFMVHLATIPIT------GTGINPARSIGAAVIFNNEKAWHNHWI 266

[Close]

BLAST of Lsi10G004590 vs. Swiss-Prot
Match: PIP25_MAIZE (Aquaporin PIP2-5 OS=Zea mays GN=PIP2-5 PE=1 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 7.8e-15
Identity = 69/251 (27.49%), Postives = 118/251 (47.01%), Query Frame = 1


Query: 52  IGAHELFSQEMWKAALTELVATAFLIFCLTSSIISCLNSHESDPK-------------LL 111
           + A EL    +++A + E VAT   ++   +++I     H++D               L 
Sbjct: 25  VDAEELTKWSLYRAVIAEFVATLLFLYITVATVIGY--KHQTDAAASGPDAACGGVGVLG 84

Query: 112 IPIAVFIILFLFLLVTFPLSGGFLSPIFAFIAALRGVITFTRAAIYILGQCLGSIIAFLM 171
           I  A   ++F+ +  T  +SGG ++P   F   L   ++  RA +YI+ QCLG+I    +
Sbjct: 85  IAWAFGGMIFILVYCTAGVSGGHINPAVTFGLFLARKVSLVRALLYIVAQCLGAICGVGL 144

Query: 172 IKDAMSPDVADKYSLGGCTIRGTGDTPGIGLTTALVLEFACTFVVLYIGVTVVLDEKMSE 231
           +K   S     +Y  GG      G + G GL      E   TFV++Y   +    ++ + 
Sbjct: 145 VKGFQSAFYV-RYG-GGANELSAGYSKGTGLAA----EIIGTFVLVYTVFSATDPKRNAR 204

Query: 232 RLGLPMVCGMIAGSSAVAVFVST-TITGRAGYGGVGLNPARCLGPALL-RGGRLWEGHWV 288
              +P++  +  G +   V ++T  IT      G G+NPAR LG A++    + W+ HW+
Sbjct: 205 DSHVPVLAPLPIGFAVFMVHLATIPIT------GTGINPARSLGAAVIYNNDKAWDDHWI 261

[Close]

BLAST of Lsi10G004590 vs. Swiss-Prot
Match: PIP21_MAIZE (Aquaporin PIP2-1 OS=Zea mays GN=PIP2-1 PE=1 SV=2)

HSP 1 Score: 81.6 bits (200), Expect = 1.7e-14
Identity = 69/250 (27.60%), Postives = 118/250 (47.20%), Query Frame = 1


Query: 52  IGAHELFSQEMWKAALTELVATAFLIFCLTSSIISCLNSHESDPK-------------LL 111
           I A EL S  +++A + E +AT   ++   +++I     H++D               L 
Sbjct: 30  IDAAELGSWSLYRAVIAEFIATLLFLYITVATVIGY--KHQTDASASGADAACGGVGVLG 89

Query: 112 IPIAVFIILFLFLLVTFPLSGGFLSPIFAFIAALRGVITFTRAAIYILGQCLGSIIAFLM 171
           I  A   ++F+ +  T  +SGG ++P   F   L   ++  RA +YI+ QCLG+I    +
Sbjct: 90  IAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSLVRALLYIVAQCLGAICGVGL 149

Query: 172 IKDAMSPDVADKYSLGGCTIRGTGDTPGIGLTTALVLEFACTFVVLYIGVTVVLDEKMSE 231
           +K A      D+Y  GG     +G + G GL      E   TFV++Y   +    ++ + 
Sbjct: 150 VK-AFQSAYFDRYG-GGANSLASGYSRGTGLGA----EIIGTFVLVYTVFSATDPKRNAR 209

Query: 232 RLGLPMVCGMIAGSSAVAVFVSTTITGRAGYGGVGLNPARCLGPALL-RGGRLWEGHWVF 288
              +P++  +  G +   V ++T         G G+NPAR LG A++    + W+ HW+F
Sbjct: 210 DSHVPVLAPLPIGFAVFMVHLAT-----IPVTGTGINPARSLGAAVIYNKDKPWDDHWIF 266

[Close]

BLAST of Lsi10G004590 vs. TrEMBL
Match: A0A0A0LJ07_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G263850 PE=3 SV=1)

HSP 1 Score: 516.2 bits (1328), Expect = 3.1e-143
Identity = 265/328 (80.79%), Postives = 290/328 (88.41%), Query Frame = 1


Query: 6   AQEDLALSMEEERGFDEQNFTQERGFDEQNFTQTDSSSFLSRFLLYIGAHELFSQEMWKA 65
           AQE+  L +EE R FDEQ               + SSSFLSRFLLYIG HELFSQEMWKA
Sbjct: 2   AQEEFVLPLEEGRSFDEQT--------------SSSSSFLSRFLLYIGVHELFSQEMWKA 61

Query: 66  ALTELVATAFLIFCLTSSIISCLNSHESDPKLLIPIAVFIILFLFLLVTFPLSGGFLSPI 125
           A+TELVAT+ LIFCLT+SI+SCLNSH+SDPKLLIPIAVFIILFLFL+VTFPLSGGFLSPI
Sbjct: 62  AMTELVATSLLIFCLTTSIVSCLNSHQSDPKLLIPIAVFIILFLFLVVTFPLSGGFLSPI 121

Query: 126 FAFIAALRGVITFTRAAIYILGQCLGSIIAFLMIKDAMSPDVADKYSLGGCTIRGTGDTP 185
           FAFIAAL GVITFTRA IYIL QCLGSI+AFLMIKDAM+PDVADKYSLGGCTIRGTG+TP
Sbjct: 122 FAFIAALHGVITFTRATIYILAQCLGSILAFLMIKDAMNPDVADKYSLGGCTIRGTGETP 181

Query: 186 GIGLTTALVLEFACTFVVLYIGVTVVLDEKMSERLGLPMVCGMIAGSSAVAVFVSTTITG 245
           G+ LTTALVLEFACTFVVLY+GVTVVLD+KMSE+ GLPMVCGMIA SSAVAVFVSTTITG
Sbjct: 182 GLSLTTALVLEFACTFVVLYVGVTVVLDQKMSEQFGLPMVCGMIAASSAVAVFVSTTITG 241

Query: 246 RAGYGGVGLNPARCLGPALLRGGRLWEGHWVFWVGPFVACMVYYGFSVNLPKGQLVVEKG 305
           RAGYGGVGL+PARCLGPA+LRGG LWEGHWVFWVGPFVAC+VYYGFS+NLPKG  V  KG
Sbjct: 242 RAGYGGVGLSPARCLGPAVLRGGLLWEGHWVFWVGPFVACVVYYGFSMNLPKGLPVGAKG 301

Query: 306 EIGILKMAGGCWRQRRRKLEGKLCQNVD 334
           EIGILKM GGC R+RR+K +GKL +NV+
Sbjct: 302 EIGILKMVGGCCRRRRQKFKGKLRENVN 315

[Close]

BLAST of Lsi10G004590 vs. TrEMBL
Match: M5VHJ5_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa025096mg PE=3 SV=1)

HSP 1 Score: 361.3 bits (926), Expect = 1.3e-96
Identity = 183/273 (67.03%), Postives = 217/273 (79.49%), Query Frame = 1


Query: 42  SSFLSRFLLYIGAHELFSQEMWKAALTELVATAFLIFCLTSSIISCLNSHESDPKLLIPI 101
           +S   +FL  IGAHE FS EMW+AA TELVATA L+F LTSSIISCL S E DPKLL+PI
Sbjct: 30  NSLRLKFLATIGAHEYFSPEMWRAAFTELVATASLLFTLTSSIISCLESGEVDPKLLVPI 89

Query: 102 AVFIILFLFLLVTFPLSGGFLSPIFAFIAALRGVITFTRAAIYILGQCLGSIIAFLMIKD 161
           AVFII FLFLLVT PLSGG +SP+F FIAAL+GVITF RA+IYIL QC+GSI+ FL+IK 
Sbjct: 90  AVFIIAFLFLLVTVPLSGGHMSPVFTFIAALKGVITFARASIYILAQCIGSILGFLIIKT 149

Query: 162 AMSPDVADKYSLGGCTIRGTGDTPGIGLTTALVLEFACTFVVLYIGVTVVLDEKMSERLG 221
            M  + A KYSLGGCTI+G+G T G+G  TAL++EFACTFVVL++GVTV  D++  + LG
Sbjct: 150 VMDQNAAQKYSLGGCTIKGSGSTSGVGTQTALMVEFACTFVVLFVGVTVAFDKRRCKELG 209

Query: 222 LPMVCGMIAGSSAVAVFVSTTITGRAGYGGVGLNPARCLGPALLRGGRLWEGHWVFWVGP 281
           L MVC ++AG+ A+AVFVS T+TGR GY GVGL+PARCLGPALL+GGRLW+GHWVFWVGP
Sbjct: 210 LAMVCAVVAGAMALAVFVSITVTGRVGYAGVGLSPARCLGPALLQGGRLWDGHWVFWVGP 269

Query: 282 FVACMVYYGFSVNLPKGQLVVEKGEIGILKMAG 315
            +AC VYY  S+NLP   L   + E  ILK  G
Sbjct: 270 ILACSVYYCVSLNLPNEGLKSVEEECDILKGEG 302

[Close]

BLAST of Lsi10G004590 vs. TrEMBL
Match: A0A061E0D7_THECC (Plasma membrane intrinsic protein 1A, putative OS=Theobroma cacao GN=TCM_006707 PE=3 SV=1)

HSP 1 Score: 355.1 bits (910), Expect = 9.1e-95
Identity = 176/281 (62.63%), Postives = 215/281 (76.51%), Query Frame = 1


Query: 40  DSSSFLSRFLLYIGAHELFSQEMWKAALTELVATAFLIFCLTSSIISCLNSHESDPKLLI 99
           +  S    F   IGAHE  S EMWKAALTELVATA L+F LT+SI+SCL+SHE+DPKLL+
Sbjct: 28  ERQSLKPTFFACIGAHEFSSPEMWKAALTELVATASLMFTLTTSIVSCLDSHETDPKLLV 87

Query: 100 PIAVFIILFLFLLVTFPLSGGFLSPIFAFIAALRGVITFTRAAIYILGQCLGSIIAFLMI 159
           P AVFII FLFL+VT PLSGG +SP+F FIAAL+G+IT  RA+IYIL QC+GS+  FL++
Sbjct: 88  PFAVFIIAFLFLMVTVPLSGGHMSPVFTFIAALKGIITLARASIYILAQCVGSLTGFLIL 147

Query: 160 KDAMSPDVADKYSLGGCTIRGTGDTPGIGLTTALVLEFACTFVVLYIGVTVVLDEKMSER 219
              M  D A +YSLGGCTI G G T GI   TAL+LEF CTF+VL +GVT+  D++ S+ 
Sbjct: 148 NSVMDQDAAKRYSLGGCTINGNGSTSGISPGTALILEFCCTFLVLLVGVTIAFDKRRSKE 207

Query: 220 LGLPMVCGMIAGSSAVAVFVSTTITGRAGYGGVGLNPARCLGPALLRGGRLWEGHWVFWV 279
           LGLPMVC ++AG+ AVAVFVS ++TGRAGY GVGLNP+RCLGPALLRGG+LW+GHWVFWV
Sbjct: 208 LGLPMVCAVVAGAMAVAVFVSISVTGRAGYAGVGLNPSRCLGPALLRGGQLWDGHWVFWV 267

Query: 280 GPFVACMVYYGFSVNLPKGQLVVEKGEIGILKMAGG---CW 318
           GP  AC++YY F+  LPK  LV   GE  ++ +A     CW
Sbjct: 268 GPCFACIIYYCFTKGLPKEGLVWADGERDVVNLAAPALCCW 308

[Close]

BLAST of Lsi10G004590 vs. TrEMBL
Match: W9QGF8_9ROSA (Aquaporin PIP-type OS=Morus notabilis GN=L484_003232 PE=3 SV=1)

HSP 1 Score: 350.9 bits (899), Expect = 1.7e-93
Identity = 186/309 (60.19%), Postives = 234/309 (75.73%), Query Frame = 1


Query: 13  SMEEERGFDEQNFTQERGFDEQNFTQTDSS-SFLSRFLLYIGAHELFSQEMWKAALTELV 72
           S+E +  F  Q   +E    +   T++D   S  ++FL  IGAHE FS EMW+AALTE V
Sbjct: 10  SVEADPQFRRQVEHREASSTDNRLTRSDHDYSLRTKFLACIGAHEFFSPEMWRAALTESV 69

Query: 73  ATAFLIFCLTSSIISCLNSHESDPKLLIPIAVFIILFLFLLVTFPLSGGFLSPIFAFIAA 132
           ATA L+F LT SIISCL+SH++DPKLL+P AVFII FLFL+VT PL+GG +SP+F FI+A
Sbjct: 70  ATACLMFSLTCSIISCLDSHQADPKLLVPFAVFIIAFLFLIVTVPLTGGHMSPVFTFISA 129

Query: 133 LRGVITFTRAAIYILGQCLGSIIAFLMIKDAMSPDVADKYSLGGCTIRGTGDTPGIGLTT 192
           L+GVIT +RA+IY+L QC+GS++ FL+IK AM+   A+KY LGGC I G G    +   T
Sbjct: 130 LKGVITLSRASIYVLAQCVGSLMGFLVIKAAMNKPAAEKYFLGGCGIMGHGGANRLEPNT 189

Query: 193 ALVLEFACTFVVLYIGVTVVLDEKMSERLGLPMVCGMIAGSSAVAVFVSTTITGRAGYGG 252
           ALVLEFACTFVVL++GVTV LDE+  + LGL MVC ++AGS  +AVFVS T+TGRAGY G
Sbjct: 190 ALVLEFACTFVVLFVGVTVALDERRCKELGLVMVCVVVAGSMGLAVFVSITVTGRAGYAG 249

Query: 253 VGLNPARCLGPALLRGGRLWEGHWVFWVGPFVACMVYYGFSVNLPK-GQLVVEKG--EIG 312
           VGLNPARCLGPALL GG LW GHWVFWVGPF+AC +YYG S+NLP+ G  +V+ G  E  
Sbjct: 250 VGLNPARCLGPALLIGGELWYGHWVFWVGPFLACSLYYGISMNLPRVGVGLVDGGEYEYD 309

Query: 313 ILKMAGGCW 318
           I+K++G C+
Sbjct: 310 IVKLSGACF 318

[Close]

BLAST of Lsi10G004590 vs. TrEMBL
Match: A0A067DYX4_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g045670mg PE=3 SV=1)

HSP 1 Score: 337.8 bits (865), Expect = 1.5e-89
Identity = 181/311 (58.20%), Postives = 227/311 (72.99%), Query Frame = 1


Query: 9   DLALSMEEERGFDEQNFTQERGFDEQNFTQTDSSSFLSRFLLYIGAHELFSQEMWKAALT 68
           DL +S E       Q F+ E+  +  N T T    FL+     IG +E FS +MW+AA T
Sbjct: 2   DLIVSHEAADPLPSQGFSIEKN-EIMNGTITRREMFLA----CIGFYEFFSPKMWRAAFT 61

Query: 69  ELVATAFLIFCLTSSIISCLNSHESDPKLLIPIAVFIILFLFLLVTFPLSGGFLSPIFAF 128
           ELVATAFL+F LT+SIISCL+SH S+ KLL+PIAVFII FLFL+VT PLSGG +SP+F F
Sbjct: 62  ELVATAFLVFTLTTSIISCLDSHVSEQKLLVPIAVFIIAFLFLMVTVPLSGGHMSPVFTF 121

Query: 129 IAALRGVITFTRAAIYILGQCLGSIIAFLMIKDAMSPDVADKYSLGGCTIRGTGDTPGIG 188
           IAAL+G++T  RAA Y+L QCLGSI+ FL+I   MS + A +YSLGGC I G G + GI 
Sbjct: 122 IAALQGIVTLARAATYVLAQCLGSIVGFLIINSVMSHNAARRYSLGGCLIAGNGTSAGIS 181

Query: 189 LTTALVLEFACTFVVLYIGVTVVLDEKMSERLGLPMVCGMIAGSSAVAVFVSTTITGRAG 248
             TAL+LEF CTFVVL++GVT+  D++  + LGL +VC ++AG+ A+AVFVS T+T + G
Sbjct: 182 AETALILEFTCTFVVLFVGVTIAFDKRRCKELGLVVVCAIVAGAMAIAVFVSITVTQKPG 241

Query: 249 YGGVGLNPARCLGPALLRGGRLWEGHWVFWVGPFVACMVYYGFSVNLPKGQLVVE-KGEI 308
           Y GVGLNPARCLGPALL GG LW+GHWVFWVGPF+AC+VYYGF+  LP+  LV E K E 
Sbjct: 242 YAGVGLNPARCLGPALLHGGPLWKGHWVFWVGPFLACVVYYGFAKTLPEEGLVWEDKFEH 301

Query: 309 GILKMAGGCWR 319
            I+ +A   WR
Sbjct: 302 DIINLA-RVWR 306

[Close]

BLAST of Lsi10G004590 vs. TAIR10
Match: AT2G37170.1 (AT2G37170.1 plasma membrane intrinsic protein 2)

HSP 1 Score: 80.5 bits (197), Expect = 2.2e-15
Identity = 69/250 (27.60%), Postives = 115/250 (46.00%), Query Frame = 1


Query: 51  YIGAHELFSQEMWKAALTELVATAFLIFCLTSSIISCLNSHESDPK-----------LLI 110
           +  A EL    +++A + E VAT   ++    ++I      +SD K           L I
Sbjct: 24  FFDADELTKWSLYRAVIAEFVATLLFLYITVLTVIGY--KIQSDTKAGGVDCGGVGILGI 83

Query: 111 PIAVFIILFLFLLVTFPLSGGFLSPIFAFIAALRGVITFTRAAIYILGQCLGSIIAFLMI 170
             A   ++F+ +  T  +SGG ++P   F   L   ++  RA +Y++ QCLG+I     +
Sbjct: 84  AWAFGGMIFILVYCTAGISGGHINPAVTFGLFLARKVSLIRAVLYMVAQCLGAICGVGFV 143

Query: 171 KDAMSPDVADKYSLGGCTIRGTGDTPGIGLTTALVLEFACTFVVLYIGVTVVLDEKMSER 230
           K A      D+Y  GG      G   G GL      E   TFV++Y   +    ++ +  
Sbjct: 144 K-AFQSSYYDRYG-GGANSLADGYNTGTGLAA----EIIGTFVLVYTVFSATDPKRNARD 203

Query: 231 LGLPMVCGMIAGSSAVAVFVST-TITGRAGYGGVGLNPARCLGPALL-RGGRLWEGHWVF 288
             +P++  +  G +   V ++T  IT      G G+NPAR  G A++    + W+ HW+F
Sbjct: 204 SHVPVLAPLPIGFAVFMVHLATIPIT------GTGINPARSFGAAVIYNKSKPWDDHWIF 259

[Close]

BLAST of Lsi10G004590 vs. TAIR10
Match: AT3G53420.1 (AT3G53420.1 plasma membrane intrinsic protein 2A)

HSP 1 Score: 79.0 bits (193), Expect = 6.3e-15
Identity = 67/248 (27.02%), Postives = 113/248 (45.56%), Query Frame = 1


Query: 51  YIGAHELFSQEMWKAALTELVATAFLIFCLTSSIISCLNSHESDPK---------LLIPI 110
           +I   EL     ++A + E VAT   ++    ++I      ++D           L I  
Sbjct: 26  FIDGAELKKWSFYRAVIAEFVATLLFLYITVLTVIGYKIQSDTDAGGVDCGGVGILGIAW 85

Query: 111 AVFIILFLFLLVTFPLSGGFLSPIFAFIAALRGVITFTRAAIYILGQCLGSIIAFLMIKD 170
           A   ++F+ +  T  +SGG ++P   F   L   ++  RA +YI+ QCLG+I     +K 
Sbjct: 86  AFGGMIFILVYCTAGISGGHINPAVTFGLFLARKVSLPRALLYIIAQCLGAICGVGFVK- 145

Query: 171 AMSPDVADKYSLGGCTIRGTGDTPGIGLTTALVLEFACTFVVLYIGVTVVLDEKMSERLG 230
           A       +Y  GG      G + G GL      E   TFV++Y   +    ++ +    
Sbjct: 146 AFQSSYYTRYG-GGANSLADGYSTGTGLAA----EIIGTFVLVYTVFSATDPKRSARDSH 205

Query: 231 LPMVCGMIAGSSAVAVFVST-TITGRAGYGGVGLNPARCLGPALL-RGGRLWEGHWVFWV 288
           +P++  +  G +   V ++T  IT      G G+NPAR  G A++    + W+ HW+FWV
Sbjct: 206 VPVLAPLPIGFAVFMVHLATIPIT------GTGINPARSFGAAVIYNKSKPWDDHWIFWV 261

[Close]

BLAST of Lsi10G004590 vs. TAIR10
Match: AT2G37180.1 (AT2G37180.1 Aquaporin-like superfamily protein)

HSP 1 Score: 77.4 bits (189), Expect = 1.8e-14
Identity = 68/250 (27.20%), Postives = 114/250 (45.60%), Query Frame = 1


Query: 51  YIGAHELFSQEMWKAALTELVATAFLIFCLTSSIISCLNSHESDPK-----------LLI 110
           +  A EL    +++A + E VAT   ++    ++I      +SD K           L I
Sbjct: 24  FFDAEELTKWSLYRAVIAEFVATLLFLYVTVLTVIGY--KIQSDTKAGGVDCGGVGILGI 83

Query: 111 PIAVFIILFLFLLVTFPLSGGFLSPIFAFIAALRGVITFTRAAIYILGQCLGSIIAFLMI 170
             A   ++F+ +  T  +SGG ++P   F   L   ++  RA +Y++ QCLG+I     +
Sbjct: 84  AWAFGGMIFILVYCTAGISGGHINPAVTFGLFLARKVSLIRAVLYMVAQCLGAICGVGFV 143

Query: 171 KDAMSPDVADKYSLGGCTIRGTGDTPGIGLTTALVLEFACTFVVLYIGVTVVLDEKMSER 230
           K   S    + Y  GG      G   G GL      E   TFV++Y   +    ++ +  
Sbjct: 144 KAFQSSHYVN-YG-GGANFLADGYNTGTGLAA----EIIGTFVLVYTVFSATDPKRNARD 203

Query: 231 LGLPMVCGMIAGSSAVAVFVST-TITGRAGYGGVGLNPARCLGPALL-RGGRLWEGHWVF 288
             +P++  +  G +   V ++T  IT      G G+NPAR  G A++    + W+ HW+F
Sbjct: 204 SHVPVLAPLPIGFAVFMVHLATIPIT------GTGINPARSFGAAVIFNKSKPWDDHWIF 259

[Close]

BLAST of Lsi10G004590 vs. TAIR10
Match: AT2G16850.1 (AT2G16850.1 plasma membrane intrinsic protein 2;8)

HSP 1 Score: 76.3 bits (186), Expect = 4.1e-14
Identity = 66/238 (27.73%), Postives = 114/238 (47.90%), Query Frame = 1


Query: 56  ELFSQEMWKAALTELVATAFLIFCLTSSIISCLNSHESDP-----KLLIPIAVFIILFLF 115
           EL     ++A + E +AT   ++   +++I   + +++ P      L I  A   ++F+ 
Sbjct: 28  ELKLWSFYRAIIAEFIATLLFLYVTVATVIG--HKNQTGPCGGVGLLGIAWAFGGMIFVL 87

Query: 116 LLVTFPLSGGFLSPIFAFIAALRGVITFTRAAIYILGQCLGSIIAFLMIKDAMSPDVADK 175
           +  T  +SGG ++P   F   L   ++  RA  Y++ QCLG+I    ++K  M      K
Sbjct: 88  VYCTAGISGGHINPAVTFGLFLARKVSLPRAVAYMVAQCLGAICGVGLVKAFMMTPY--K 147

Query: 176 YSLGGCTIRGTGDTPGIGLTTALVLEFACTFVVLYIGVTVVLDEKMSERLGLPMVCGMIA 235
              GG      G + G    TAL  E   TFV++Y   +    ++ +    +P++  +  
Sbjct: 148 RLGGGANTVADGYSTG----TALGAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLPI 207

Query: 236 GSSAVAVFVSTT-ITGRAGYGGVGLNPARCLGPALLRGG-RLWEGHWVFWVGPFVACM 287
           G +   V ++T  ITG       G+NPAR  G A++    + W+ HW+FWVGPFV  +
Sbjct: 208 GFAVFMVHLATIPITG------TGINPARSFGAAVIYNNEKAWDDHWIFWVGPFVGAL 251

[Close]

BLAST of Lsi10G004590 vs. TAIR10
Match: AT3G61430.1 (AT3G61430.1 plasma membrane intrinsic protein 1A)

HSP 1 Score: 73.9 bits (180), Expect = 2.0e-13
Identity = 67/237 (28.27%), Postives = 109/237 (45.99%), Query Frame = 1


Query: 56  ELFSQEMWKAALTELVATAFLIFCLTSSIISCLNSHESDPKLLIPIAVFII-------LF 115
           EL S   W+A + E +AT   ++    +++    S    P +   + +  I       +F
Sbjct: 44  ELSSWSFWRAGIAEFIATFLFLYITVLTVMGVKRS----PNMCASVGIQGIAWAFGGMIF 103

Query: 116 LFLLVTFPLSGGFLSPIFAFIAALRGVITFTRAAIYILGQCLGSIIAFLMIKDAMSPDVA 175
             +  T  +SGG ++P   F   L   ++ TRA  YI+ QCLG+I    ++K    P   
Sbjct: 104 ALVYCTAGISGGHINPAVTFGLFLARKLSLTRALYYIVMQCLGAICGAGVVK-GFQPKQY 163

Query: 176 DKYSLGGCTIRGTGDTPGIGLTTALVLEFACTFVVLYIGVTVVLDEKMSERLGLPMVCGM 235
                GG      G T G GL   ++     TFV++Y   +    ++ +    +P++  +
Sbjct: 164 QALG-GGANTVAHGYTKGSGLGAEII----GTFVLVYTVFSATDAKRNARDSHVPILAPL 223

Query: 236 IAGSSAVAVFVSTT-ITGRAGYGGVGLNPARCLGPALLRG-GRLWEGHWVFWVGPFV 284
             G +   V ++T  ITG       G+NPAR LG A++      W+ HWVFWVGPF+
Sbjct: 224 PIGFAVFLVHLATIPITG------TGINPARSLGAAIIYNKDHSWDDHWVFWVGPFI 264

[Close]

BLAST of Lsi10G004590 vs. NCBI nr
Match: gi|449464720|ref|XP_004150077.1| (PREDICTED: aquaporin TIP1-2-like [Cucumis sativus])

HSP 1 Score: 516.2 bits (1328), Expect = 4.4e-143
Identity = 265/328 (80.79%), Postives = 290/328 (88.41%), Query Frame = 1


Query: 6   AQEDLALSMEEERGFDEQNFTQERGFDEQNFTQTDSSSFLSRFLLYIGAHELFSQEMWKA 65
           AQE+  L +EE R FDEQ               + SSSFLSRFLLYIG HELFSQEMWKA
Sbjct: 2   AQEEFVLPLEEGRSFDEQT--------------SSSSSFLSRFLLYIGVHELFSQEMWKA 61

Query: 66  ALTELVATAFLIFCLTSSIISCLNSHESDPKLLIPIAVFIILFLFLLVTFPLSGGFLSPI 125
           A+TELVAT+ LIFCLT+SI+SCLNSH+SDPKLLIPIAVFIILFLFL+VTFPLSGGFLSPI
Sbjct: 62  AMTELVATSLLIFCLTTSIVSCLNSHQSDPKLLIPIAVFIILFLFLVVTFPLSGGFLSPI 121

Query: 126 FAFIAALRGVITFTRAAIYILGQCLGSIIAFLMIKDAMSPDVADKYSLGGCTIRGTGDTP 185
           FAFIAAL GVITFTRA IYIL QCLGSI+AFLMIKDAM+PDVADKYSLGGCTIRGTG+TP
Sbjct: 122 FAFIAALHGVITFTRATIYILAQCLGSILAFLMIKDAMNPDVADKYSLGGCTIRGTGETP 181

Query: 186 GIGLTTALVLEFACTFVVLYIGVTVVLDEKMSERLGLPMVCGMIAGSSAVAVFVSTTITG 245
           G+ LTTALVLEFACTFVVLY+GVTVVLD+KMSE+ GLPMVCGMIA SSAVAVFVSTTITG
Sbjct: 182 GLSLTTALVLEFACTFVVLYVGVTVVLDQKMSEQFGLPMVCGMIAASSAVAVFVSTTITG 241

Query: 246 RAGYGGVGLNPARCLGPALLRGGRLWEGHWVFWVGPFVACMVYYGFSVNLPKGQLVVEKG 305
           RAGYGGVGL+PARCLGPA+LRGG LWEGHWVFWVGPFVAC+VYYGFS+NLPKG  V  KG
Sbjct: 242 RAGYGGVGLSPARCLGPAVLRGGLLWEGHWVFWVGPFVACVVYYGFSMNLPKGLPVGAKG 301

Query: 306 EIGILKMAGGCWRQRRRKLEGKLCQNVD 334
           EIGILKM GGC R+RR+K +GKL +NV+
Sbjct: 302 EIGILKMVGGCCRRRRQKFKGKLRENVN 315

[Close]

BLAST of Lsi10G004590 vs. NCBI nr
Match: gi|659115609|ref|XP_008457641.1| (PREDICTED: aquaporin TIP1-2-like [Cucumis melo])

HSP 1 Score: 509.6 bits (1311), Expect = 4.1e-141
Identity = 265/329 (80.55%), Postives = 288/329 (87.54%), Query Frame = 1


Query: 6   AQEDLALSMEEERGFDEQNFTQERGFDEQNFTQTDSSSFLSRFLLYIGAHELFSQEMWKA 65
           AQE+ AL +EEE G         R FDEQ    + SSSFLSRFL+YIGAHELFSQEMWKA
Sbjct: 2   AQEEFALPLEEEEG---------RSFDEQT---SSSSSFLSRFLIYIGAHELFSQEMWKA 61

Query: 66  ALTELVATAFLIFCLTSSIISCLNSHESDPKLLIPIAVFIILFLFLLVTFPLSGGFLSPI 125
           A+TELVATA LIFCLT+SI+SCLNSH+SDPKLLIP AVFIILFLFL+VTFPLSGGFLSPI
Sbjct: 62  AMTELVATALLIFCLTTSIVSCLNSHQSDPKLLIPFAVFIILFLFLVVTFPLSGGFLSPI 121

Query: 126 FAFIAALRGVITFTRAAIYILGQCLGSIIAFLMIKDAMSPDVADKYSLGGCTIRGTGDTP 185
           FAFIAAL GVITFTRA +YIL QCL SI+AFLMIKDAMSPDVADKYSLGGCTIRGTG+TP
Sbjct: 122 FAFIAALHGVITFTRATVYILAQCLASILAFLMIKDAMSPDVADKYSLGGCTIRGTGETP 181

Query: 186 GIGLTTALVLEFACTFVVLYIGVTVVLDEKMSERLGLPMVCGMIAGSSAVAVFVSTTITG 245
           G+ +TTAL+LEFACTFVVLY+GVTVVLD+KMSER GLPMVCGMIA SSAVAVFVSTTITG
Sbjct: 182 GLSVTTALILEFACTFVVLYVGVTVVLDQKMSERFGLPMVCGMIAASSAVAVFVSTTITG 241

Query: 246 RAGYGGVGLNPARCLGPALLRGGRLWEGHWVFWVGPFVACMVYYGFSVNLPKGQLVVEKG 305
           RAGYGGVGL+PARCLGPA+LRGG LWEGHWVFWVGPF AC+VYYGFS NLP G LV  KG
Sbjct: 242 RAGYGGVGLSPARCLGPAVLRGGLLWEGHWVFWVGPFAACVVYYGFSKNLPNGVLVGAKG 301

Query: 306 EIGILKMAGG-CWRQRRRKLEGKLCQNVD 334
           EIGILKM GG CWR+RR+KL     +NVD
Sbjct: 302 EIGILKMVGGRCWRRRRQKLR----ENVD 314

[Close]

BLAST of Lsi10G004590 vs. NCBI nr
Match: gi|595791193|ref|XP_007199345.1| (hypothetical protein PRUPE_ppa025096mg [Prunus persica])

HSP 1 Score: 361.3 bits (926), Expect = 1.8e-96
Identity = 183/273 (67.03%), Postives = 217/273 (79.49%), Query Frame = 1


Query: 42  SSFLSRFLLYIGAHELFSQEMWKAALTELVATAFLIFCLTSSIISCLNSHESDPKLLIPI 101
           +S   +FL  IGAHE FS EMW+AA TELVATA L+F LTSSIISCL S E DPKLL+PI
Sbjct: 30  NSLRLKFLATIGAHEYFSPEMWRAAFTELVATASLLFTLTSSIISCLESGEVDPKLLVPI 89

Query: 102 AVFIILFLFLLVTFPLSGGFLSPIFAFIAALRGVITFTRAAIYILGQCLGSIIAFLMIKD 161
           AVFII FLFLLVT PLSGG +SP+F FIAAL+GVITF RA+IYIL QC+GSI+ FL+IK 
Sbjct: 90  AVFIIAFLFLLVTVPLSGGHMSPVFTFIAALKGVITFARASIYILAQCIGSILGFLIIKT 149

Query: 162 AMSPDVADKYSLGGCTIRGTGDTPGIGLTTALVLEFACTFVVLYIGVTVVLDEKMSERLG 221
            M  + A KYSLGGCTI+G+G T G+G  TAL++EFACTFVVL++GVTV  D++  + LG
Sbjct: 150 VMDQNAAQKYSLGGCTIKGSGSTSGVGTQTALMVEFACTFVVLFVGVTVAFDKRRCKELG 209

Query: 222 LPMVCGMIAGSSAVAVFVSTTITGRAGYGGVGLNPARCLGPALLRGGRLWEGHWVFWVGP 281
           L MVC ++AG+ A+AVFVS T+TGR GY GVGL+PARCLGPALL+GGRLW+GHWVFWVGP
Sbjct: 210 LAMVCAVVAGAMALAVFVSITVTGRVGYAGVGLSPARCLGPALLQGGRLWDGHWVFWVGP 269

Query: 282 FVACMVYYGFSVNLPKGQLVVEKGEIGILKMAG 315
            +AC VYY  S+NLP   L   + E  ILK  G
Sbjct: 270 ILACSVYYCVSLNLPNEGLKSVEEECDILKGEG 302

[Close]

BLAST of Lsi10G004590 vs. NCBI nr
Match: gi|645261280|ref|XP_008236217.1| (PREDICTED: aquaporin TIP1-2-like [Prunus mume])

HSP 1 Score: 358.6 bits (919), Expect = 1.2e-95
Identity = 182/273 (66.67%), Postives = 214/273 (78.39%), Query Frame = 1


Query: 42  SSFLSRFLLYIGAHELFSQEMWKAALTELVATAFLIFCLTSSIISCLNSHESDPKLLIPI 101
           +S   +FL  IGAHE FS EMW+AA TELVATA L+F LTSSIISCL S E DPKLL+PI
Sbjct: 30  NSLRLKFLTAIGAHEYFSPEMWRAAFTELVATASLLFTLTSSIISCLESGEVDPKLLVPI 89

Query: 102 AVFIILFLFLLVTFPLSGGFLSPIFAFIAALRGVITFTRAAIYILGQCLGSIIAFLMIKD 161
           AVFII FLFLLVT PLSGG +SP+F FIAA +GVITF RA+ YIL QC+GSI+ FL+IK 
Sbjct: 90  AVFIIAFLFLLVTVPLSGGHMSPVFTFIAAFKGVITFVRASTYILAQCIGSILGFLIIKT 149

Query: 162 AMSPDVADKYSLGGCTIRGTGDTPGIGLTTALVLEFACTFVVLYIGVTVVLDEKMSERLG 221
            M  + A KYSLGGCTI+G+G T G+G  TAL++EFACTFVVL++GVTV  D++  + LG
Sbjct: 150 VMDQNAAQKYSLGGCTIKGSGSTSGVGTQTALMVEFACTFVVLFVGVTVAFDKRRCKELG 209

Query: 222 LPMVCGMIAGSSAVAVFVSTTITGRAGYGGVGLNPARCLGPALLRGGRLWEGHWVFWVGP 281
           L MVC ++AG+ A+AVFVS T+TGR GY GVGLNPARCLGPALL GGRLW+GHWVFWVGP
Sbjct: 210 LAMVCAVVAGAMALAVFVSITVTGRVGYAGVGLNPARCLGPALLLGGRLWDGHWVFWVGP 269

Query: 282 FVACMVYYGFSVNLPKGQLVVEKGEIGILKMAG 315
            +AC VYY  S+NLP   L   + E  ILK  G
Sbjct: 270 ILACSVYYCVSLNLPNEGLKSVEEECDILKGEG 302

[Close]

BLAST of Lsi10G004590 vs. NCBI nr
Match: gi|590684847|ref|XP_007041950.1| (Plasma membrane intrinsic protein 1A, putative [Theobroma cacao])

HSP 1 Score: 355.1 bits (910), Expect = 1.3e-94
Identity = 176/281 (62.63%), Postives = 215/281 (76.51%), Query Frame = 1


Query: 40  DSSSFLSRFLLYIGAHELFSQEMWKAALTELVATAFLIFCLTSSIISCLNSHESDPKLLI 99
           +  S    F   IGAHE  S EMWKAALTELVATA L+F LT+SI+SCL+SHE+DPKLL+
Sbjct: 28  ERQSLKPTFFACIGAHEFSSPEMWKAALTELVATASLMFTLTTSIVSCLDSHETDPKLLV 87

Query: 100 PIAVFIILFLFLLVTFPLSGGFLSPIFAFIAALRGVITFTRAAIYILGQCLGSIIAFLMI 159
           P AVFII FLFL+VT PLSGG +SP+F FIAAL+G+IT  RA+IYIL QC+GS+  FL++
Sbjct: 88  PFAVFIIAFLFLMVTVPLSGGHMSPVFTFIAALKGIITLARASIYILAQCVGSLTGFLIL 147

Query: 160 KDAMSPDVADKYSLGGCTIRGTGDTPGIGLTTALVLEFACTFVVLYIGVTVVLDEKMSER 219
              M  D A +YSLGGCTI G G T GI   TAL+LEF CTF+VL +GVT+  D++ S+ 
Sbjct: 148 NSVMDQDAAKRYSLGGCTINGNGSTSGISPGTALILEFCCTFLVLLVGVTIAFDKRRSKE 207

Query: 220 LGLPMVCGMIAGSSAVAVFVSTTITGRAGYGGVGLNPARCLGPALLRGGRLWEGHWVFWV 279
           LGLPMVC ++AG+ AVAVFVS ++TGRAGY GVGLNP+RCLGPALLRGG+LW+GHWVFWV
Sbjct: 208 LGLPMVCAVVAGAMAVAVFVSISVTGRAGYAGVGLNPSRCLGPALLRGGQLWDGHWVFWV 267

Query: 280 GPFVACMVYYGFSVNLPKGQLVVEKGEIGILKMAGG---CW 318
           GP  AC++YY F+  LPK  LV   GE  ++ +A     CW
Sbjct: 268 GPCFACIIYYCFTKGLPKEGLVWADGERDVVNLAAPALCCW 308

The following BLAST results are available for this feature:

BLAST of Lsi10G004590 vs. Swiss-Prot


Match Name	E-value	Identity	Description
TIP12_MAIZE	4.1e-16	30.33	Aquaporin TIP1-2 OS=Zea mays GN=TIP1-2 PE=2 SV=1	[more]
TIP12_ORYSJ	2.1e-15	28.87	Probable aquaporin TIP1-2 OS=Oryza sativa subsp. japonica GN=TIP1-2 PE=2 SV=1	[more]
PIP21_ORYSJ	2.1e-15	27.89	Probable aquaporin PIP2-1 OS=Oryza sativa subsp. japonica GN=PIP2-1 PE=2 SV=1	[more]
PIP25_MAIZE	7.8e-15	27.49	Aquaporin PIP2-5 OS=Zea mays GN=PIP2-5 PE=1 SV=1	[more]
PIP21_MAIZE	1.7e-14	27.60	Aquaporin PIP2-1 OS=Zea mays GN=PIP2-1 PE=1 SV=2	[more]

BLAST of Lsi10G004590 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LJ07_CUCSA	3.1e-143	80.79	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G263850 PE=3 SV=1	[more]
M5VHJ5_PRUPE	1.3e-96	67.03	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa025096mg PE=3 SV=1	[more]
A0A061E0D7_THECC	9.1e-95	62.63	Plasma membrane intrinsic protein 1A, putative OS=Theobroma cacao GN=TCM_006707 ...	[more]
W9QGF8_9ROSA	1.7e-93	60.19	Aquaporin PIP-type OS=Morus notabilis GN=L484_003232 PE=3 SV=1	[more]
A0A067DYX4_CITSI	1.5e-89	58.20	Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g045670mg PE=3 SV=1	[more]

BLAST of Lsi10G004590 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G37170.1	2.2e-15	27.60	plasma membrane intrinsic protein 2	[more]
AT3G53420.1	6.3e-15	27.02	plasma membrane intrinsic protein 2A	[more]
AT2G37180.1	1.8e-14	27.20	Aquaporin-like superfamily protein	[more]
AT2G16850.1	4.1e-14	27.73	plasma membrane intrinsic protein 2;8	[more]
AT3G61430.1	2.0e-13	28.27	plasma membrane intrinsic protein 1A	[more]

BLAST of Lsi10G004590 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449464720\|ref\|XP_004150077.1\|	4.4e-143	80.79	PREDICTED: aquaporin TIP1-2-like [Cucumis sativus]	[more]
gi\|659115609\|ref\|XP_008457641.1\|	4.1e-141	80.55	PREDICTED: aquaporin TIP1-2-like [Cucumis melo]	[more]
gi\|595791193\|ref\|XP_007199345.1\|	1.8e-96	67.03	hypothetical protein PRUPE_ppa025096mg [Prunus persica]	[more]
gi\|645261280\|ref\|XP_008236217.1\|	1.2e-95	66.67	PREDICTED: aquaporin TIP1-2-like [Prunus mume]	[more]
gi\|590684847\|ref\|XP_007041950.1\|	1.3e-94	62.63	Plasma membrane intrinsic protein 1A, putative [Theobroma cacao]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Cellular Component**
Term	Definition
GO:0016020	membrane

Vocabulary: **Biological Process**
Term	Definition
GO:0006810	transport

Vocabulary: **Molecular Function**
Term	Definition
GO:0005215	transporter activity

Vocabulary: **INTERPRO**
Term	Definition
IPR023271	Aquaporin-like
IPR000425	MIP

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006810	transport
biological_process	GO:0006621	protein retention in ER lumen
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0016020	membrane
molecular_function	GO:0005215	transporter activity
molecular_function	GO:0046923	ER retention sequence binding
molecular_function	GO:0015267	channel activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi10G004590.1	Lsi10G004590.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000425	Major intrinsic protein	PRINTS	PR00783	MINTRINSICP	coord: 275..295 score: 1.3E-18coord: 140..159 score: 1.3E-18coord: 103..127 score: 1.3E-18coord: 64..83 score: 1.3E-18coord: 195..213 score: 1.3E-18coord: 236..258 score: 1.3
IPR000425	Major intrinsic protein	PANTHER	PTHR19139	AQUAPORIN TRANSPORTER	coord: 47..312 score: 4.5E
IPR000425	Major intrinsic protein	PFAM	PF00230	MIP	coord: 59..286 score: 4.0
IPR023271	Aquaporin-like	GENE3D	G3DSA:1.20.1080.10		coord: 41..288 score: 2.2
IPR023271	Aquaporin-like	unknown	SSF81338	Aquaporin-like	coord: 60..287 score: 4.45
None	No IPR available	PANTHER	PTHR19139:SF172	SUBFAMILY NOT NAMED	coord: 47..312 score: 4.5E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi10G004590	Csa2G263850	Cucumber (Chinese Long) v2	culsiB093
Lsi10G004590	CSPI02G13080	Wild cucumber (PI 183967)	cpilsiB091
Lsi10G004590	Cucsa.169290	Cucumber (Gy14) v1	cgylsiB281
Lsi10G004590	CmaCh20G007730	Cucurbita maxima (Rimu)	cmalsiB507
Lsi10G004590	CmoCh20G007730	Cucurbita moschata (Rifu)	cmolsiB491
Lsi10G004590	Cla001670	Watermelon (97103) v1	lsiwmB065
Lsi10G004590	MELO3C020774	Melon (DHL92) v3.5.1	lsimeB054
Lsi10G004590	ClCG02G011460	Watermelon (Charleston Gray)	lsiwcgB045
Lsi10G004590	MELO3C020774.2	Melon (DHL92) v3.6.1	lsimedB056
Lsi10G004590	Cla97C02G037150	Watermelon (97103) v2	lsiwmbB044
Lsi10G004590	Bhi10G001511	Wax gourd	lsiwgoB080
Lsi10G004590	Cp4.1LG16g02880	Cucurbita pepo (Zucchini)	cpelsiB220
Lsi10G004590	CsGy2G013050	Cucumber (Gy14) v2	cgyblsiB086
Lsi10G004590	Carg16197	Silver-seed gourd	carlsiB314
Lsi10G004590	CsaV3_2G015640	Cucumber (Chinese Long) v3	cuclsiB104

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi10G004590	Bottle gourd (USVL1VR-Ls)	lsilsiB021
Lsi10G004590	Cucumber (Gy14) v1	cgylsiB617
Lsi10G004590	Cucurbita maxima (Rimu)	cmalsiB089
Lsi10G004590	Cucurbita maxima (Rimu)	cmalsiB466
Lsi10G004590	Cucurbita maxima (Rimu)	cmalsiB548
Lsi10G004590	Cucurbita moschata (Rifu)	cmolsiB073
Lsi10G004590	Cucurbita moschata (Rifu)	cmolsiB451
Lsi10G004590	Cucurbita moschata (Rifu)	cmolsiB533
Lsi10G004590	Cucurbita pepo (Zucchini)	cpelsiB046
Lsi10G004590	Cucurbita pepo (Zucchini)	cpelsiB388
Lsi10G004590	Cucurbita pepo (Zucchini)	cpelsiB436
Lsi10G004590	Cucurbita pepo (Zucchini)	cpelsiB589
Lsi10G004590	Wild cucumber (PI 183967)	cpilsiB497
Lsi10G004590	Cucumber (Chinese Long) v2	culsiB489
Lsi10G004590	Melon (DHL92) v3.5.1	lsimeB064
Lsi10G004590	Watermelon (Charleston Gray)	lsiwcgB057
Lsi10G004590	Watermelon (97103) v1	lsiwmB079
Lsi10G004590	Cucumber (Gy14) v2	cgyblsiB456
Lsi10G004590	Silver-seed gourd	carlsiB289
Lsi10G004590	Silver-seed gourd	carlsiB777