Lsi10G002950 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi10G002950
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Probable carboxylesterase 2
Location	chr10 : 4791596 .. 4792516 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATTCCCAACAAGAAGAAATCGTTTTTGAATTTCTACCAGTCTTTCGTGTCTTCAAAACCGGCCGCATTGATAGGCTCATTCTTACCGACCGCGTTCCGCCGTCCACGGATCAACTCACCGGAGTCCAATCCAAAGATTTCCTCATCGATCCACAAACCGGAGTCTCCGTCCGGATCTACCTCCCTCAACTTTCAGATTCCACCAACAAGCTTCCTCTTCTAATTTATATCCATGGCGGTGCCTTTGTCGTCGGCTCCCCTGCCTGTCTCATGTTCCACAACCATCTAATCTCCCTCGCCGCCCAAGCAAACGCCGTCGCGCTCTCCGTCGAATACCGCCTGGCACCGGAGAATAATCTCCCGGCAGCGTACGACGACGCTTGGACGGCGGTGGAATGGGCGGCGTCGCATTCCAATCACAACGGCCCTGAGATTTGGCTGAACGAGCATGCGGATTTTAATCGCGTGTTTGTTGTAGGGGAGAGCGCTGGCGCCAATATTTCGTACAATGTAATGGGCCGGGCCAGCGAAAATGAAAATGGGCTTAATGGAATGAAGGTAATTGGGCTGGGCCTTGTCCATCCGTATTTCATGATCGACAAGCCCGACAAGTTGATAGAGTATGTTTTCCCGACGTCGGCAGGTCTAGAGGATCCGAGAATAAACCCGAAGGCCAACCCGGGACTAGGGAAGTTACGGTGCAACCGCGTCCTGATATTCTCTGCGGAAAATGATTCTCTAAAATATGGAGCGATTGGGGTTCACGCAGCTTTTAAGGAGAGTGGGTTTGAAGGATCGGTAGAAATGGTAGAGACCGAGGGTGAGGGCCATGGTTTCCATCTCTTTAATCCCGACACTGAGAAGGCTCACGAGGTGGTCAAACAGCTGGCTTTCTTTATCCAATCACCATTACCATAA

mRNA sequence

ATGGATTCCCAACAAGAAGAAATCGTTTTTGAATTTCTACCAGTCTTTCGTGTCTTCAAAACCGGCCGCATTGATAGGCTCATTCTTACCGACCGCGTTCCGCCGTCCACGGATCAACTCACCGGAGTCCAATCCAAAGATTTCCTCATCGATCCACAAACCGGAGTCTCCGTCCGGATCTACCTCCCTCAACTTTCAGATTCCACCAACAAGCTTCCTCTTCTAATTTATATCCATGGCGGTGCCTTTGTCGTCGGCTCCCCTGCCTGTCTCATGTTCCACAACCATCTAATCTCCCTCGCCGCCCAAGCAAACGCCGTCGCGCTCTCCGTCGAATACCGCCTGGCACCGGAGAATAATCTCCCGGCAGCGTACGACGACGCTTGGACGGCGGTGGAATGGGCGGCGTCGCATTCCAATCACAACGGCCCTGAGATTTGGCTGAACGAGCATGCGGATTTTAATCGCGTGTTTGTTGTAGGGGAGAGCGCTGGCGCCAATATTTCGTACAATGTAATGGGCCGGGCCAGCGAAAATGAAAATGGGCTTAATGGAATGAAGGTAATTGGGCTGGGCCTTGTCCATCCGTATTTCATGATCGACAAGCCCGACAAGTTGATAGAGTATGTTTTCCCGACGTCGGCAGGTCTAGAGGATCCGAGAATAAACCCGAAGGCCAACCCGGGACTAGGGAAGTTACGGTGCAACCGCGTCCTGATATTCTCTGCGGAAAATGATTCTCTAAAATATGGAGCGATTGGGGTTCACGCAGCTTTTAAGGAGAGTGGGTTTGAAGGATCGGTAGAAATGGTAGAGACCGAGGGTGAGGGCCATGGTTTCCATCTCTTTAATCCCGACACTGAGAAGGCTCACGAGGTGGTCAAACAGCTGGCTTTCTTTATCCAATCACCATTACCATAA

Coding sequence (CDS)

ATGGATTCCCAACAAGAAGAAATCGTTTTTGAATTTCTACCAGTCTTTCGTGTCTTCAAAACCGGCCGCATTGATAGGCTCATTCTTACCGACCGCGTTCCGCCGTCCACGGATCAACTCACCGGAGTCCAATCCAAAGATTTCCTCATCGATCCACAAACCGGAGTCTCCGTCCGGATCTACCTCCCTCAACTTTCAGATTCCACCAACAAGCTTCCTCTTCTAATTTATATCCATGGCGGTGCCTTTGTCGTCGGCTCCCCTGCCTGTCTCATGTTCCACAACCATCTAATCTCCCTCGCCGCCCAAGCAAACGCCGTCGCGCTCTCCGTCGAATACCGCCTGGCACCGGAGAATAATCTCCCGGCAGCGTACGACGACGCTTGGACGGCGGTGGAATGGGCGGCGTCGCATTCCAATCACAACGGCCCTGAGATTTGGCTGAACGAGCATGCGGATTTTAATCGCGTGTTTGTTGTAGGGGAGAGCGCTGGCGCCAATATTTCGTACAATGTAATGGGCCGGGCCAGCGAAAATGAAAATGGGCTTAATGGAATGAAGGTAATTGGGCTGGGCCTTGTCCATCCGTATTTCATGATCGACAAGCCCGACAAGTTGATAGAGTATGTTTTCCCGACGTCGGCAGGTCTAGAGGATCCGAGAATAAACCCGAAGGCCAACCCGGGACTAGGGAAGTTACGGTGCAACCGCGTCCTGATATTCTCTGCGGAAAATGATTCTCTAAAATATGGAGCGATTGGGGTTCACGCAGCTTTTAAGGAGAGTGGGTTTGAAGGATCGGTAGAAATGGTAGAGACCGAGGGTGAGGGCCATGGTTTCCATCTCTTTAATCCCGACACTGAGAAGGCTCACGAGGTGGTCAAACAGCTGGCTTTCTTTATCCAATCACCATTACCATAA

Protein sequence

MDSQQEEIVFEFLPVFRVFKTGRIDRLILTDRVPPSTDQLTGVQSKDFLIDPQTGVSVRIYLPQLSDSTNKLPLLIYIHGGAFVVGSPACLMFHNHLISLAAQANAVALSVEYRLAPENNLPAAYDDAWTAVEWAASHSNHNGPEIWLNEHADFNRVFVVGESAGANISYNVMGRASENENGLNGMKVIGLGLVHPYFMIDKPDKLIEYVFPTSAGLEDPRINPKANPGLGKLRCNRVLIFSAENDSLKYGAIGVHAAFKESGFEGSVEMVETEGEGHGFHLFNPDTEKAHEVVKQLAFFIQSPLP

Homology

[Close]

BLAST of Lsi10G002950 vs. Swiss-Prot
Match: CXE2_ARATH (Probable carboxylesterase 2 OS=Arabidopsis thaliana GN=CXE2 PE=2 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 8.6e-61
Identity = 128/317 (40.38%), Postives = 194/317 (61.20%), Query Frame = 1


Query: 1   MDSQQEEIVFEFLPVFRVFKTGRIDRLILTDRVPPSTDQLTGVQSKDFLIDPQTGVSVRI 60
           M+S ++++  E LP   V   G ++RL  T+  PP  D +TGV SKD +I+P+TG+S RI
Sbjct: 1   MESTKKQVSLELLPWLVVHTDGTVERLAGTEVCPPGLDPITGVFSKDIIIEPKTGLSARI 60

Query: 61  YLPQLSDSTNKLPLLIYIHGGAFVVGSPACLMFHNHLISLAAQANAVALSVEYRLAPENN 120
           Y P       K+PL++Y HGGAF++ S +   +H  L  +  QAN +A+SV YRLAPE+ 
Sbjct: 61  YRPFSIQPGQKIPLMLYFHGGAFLISSTSFPSYHTSLNKIVNQANVIAVSVNYRLAPEHP 120

Query: 121 LPAAYDDAWTAVEWAASHSNHNGPEIWLNEHADFNRVFVVGESAGANISYNVMGRASENE 180
           LP AY+D+WTA++   + +     E W+N++AD + +F+VG+SAGANIS+++  RA +++
Sbjct: 121 LPTAYEDSWTALKNIQAIN-----EPWINDYADLDSLFLVGDSAGANISHHLAFRAKQSD 180

Query: 181 NGLNGMKVIGLGLVHPYFMIDKP--------------DKLIEYVFPTSAGLEDPRINPKA 240
             L   K+ G+G++HPYF   +P              D   E+V P+  G +DP INP A
Sbjct: 181 QTL---KIKGIGMIHPYFWGTQPIGAEIKDEARKQMVDGWWEFVCPSEKGSDDPWINPFA 240

Query: 241 --NPGLGKLRCNRVLIFSAENDSLKYGAIGVHAAFKESGFEGSVEMVETEGEGHGFHLFN 300
             +P LG L C RV+I  AE D L       +    +S ++G VE++ET+ + H FH+F 
Sbjct: 241 DGSPDLGGLGCERVMITVAEKDILNERGKMYYERLVKSEWKGKVEIMETKEKDHVFHIFE 300

Query: 301 PDTEKAHEVVKQLAFFI 302
           PD ++A E+V+ LA FI
Sbjct: 301 PDCDEAMEMVRCLALFI 309

[Close]

BLAST of Lsi10G002950 vs. Swiss-Prot
Match: CXE1_ARATH (Probable carboxylesterase 1 OS=Arabidopsis thaliana GN=CXE1 PE=2 SV=1)

HSP 1 Score: 234.6 bits (597), Expect = 1.5e-60
Identity = 128/318 (40.25%), Postives = 190/318 (59.75%), Query Frame = 1


Query: 1   MDSQQEEIVFEFLPVFRVFKTGRIDRLILTDRVPPSTDQLTGVQSKDFLIDPQTGVSVRI 60
           MDS   EI F++ P FR+FK G I+RL+    VPPS +   GV SKD +  P+  +S+RI
Sbjct: 1   MDS---EIAFDYSPRFRIFKNGGIERLVPETFVPPSLNPENGVVSKDAVYSPEKNLSLRI 60

Query: 61  YLPQLS---DSTNKLPLLIYIHGGAFVVGSPACLMFHNHLISLAAQANAVALSVEYRLAP 120
           YLPQ S       K+PLL+Y HGG F++ +    ++H  L S  +  + +A+SVEYR AP
Sbjct: 61  YLPQNSVYETGEKKIPLLVYFHGGGFIMETAFSPIYHTFLTSAVSATDCIAVSVEYRRAP 120

Query: 121 ENNLPAAYDDAWTAVEWAASHSNHNGPEIWLNEHADFNRVFVVGESAGANISYNVMGRAS 180
           E+ +P  Y+D+W A++W  +H   +GPE WLN+HADF++VF+ G+SAGANI++++  R  
Sbjct: 121 EHPIPTLYEDSWDAIQWIFTHITRSGPEDWLNKHADFSKVFLAGDSAGANIAHHMAIRVD 180

Query: 181 ENENGLNGMKVIGLGLVHPYFM------------IDKPDKLIEYVFPTSA-GLEDPRINP 240
           + +      K+ G+ L HPYF+            +   ++L     P S  G+EDP IN 
Sbjct: 181 KEKLPPENFKISGMILFHPYFLSKALIEEMEVEAMRYYERLWRIASPDSGNGVEDPWINV 240

Query: 241 KANPGLGKLRCNRVLIFSAENDSLKYGAIGVHAAFKESGFEGSVEMVETEGEGHGFHLFN 300
             +   G L C RVL+  A ND L  G     A  ++SG+ G V+++ET+ EGH FHL +
Sbjct: 241 VGSDLTG-LGCRRVLVMVAGNDVLARGGWSYVAELEKSGWIGKVKVMETKEEGHVFHLRD 300

Query: 301 PDTEKAHEVVKQLAFFIQ 303
           PD+E A  V++  A F++
Sbjct: 301 PDSENARRVLRNFAEFLK 314

[Close]

BLAST of Lsi10G002950 vs. Swiss-Prot
Match: CXE5_ARATH (Probable carboxylesterase 5 OS=Arabidopsis thaliana GN=CXE5 PE=2 SV=1)

HSP 1 Score: 232.6 bits (592), Expect = 5.6e-60
Identity = 128/318 (40.25%), Postives = 189/318 (59.43%), Query Frame = 1


Query: 5   QEEIVFEFLPVFRVFKTGRIDRLILTDRVPPSTDQLTGVQSKDFLIDPQTGVSVRIYLPQ 64
           + EI  EFLP  R++K GR++RLI TD +P S D    V SKD +  P+  +SVR++LP 
Sbjct: 2   ESEIASEFLPFCRIYKDGRVERLIGTDTIPASLDPTYDVVSKDVIYSPENNLSVRLFLPH 61

Query: 65  LSDST---NKLPLLIYIHGGAFVVGSPACLMFHNHLISLAAQANAVALSVEYRLAPENNL 124
            S      NKLPLLIYIHGGA+++ SP   ++HN+L  +   AN +A+SV+YR APE+ +
Sbjct: 62  KSTKLTAGNKLPLLIYIHGGAWIIESPFSPLYHNYLTEVVKSANCLAVSVQYRRAPEDPV 121

Query: 125 PAAYDDAWTAVEWAASHSNHNGPEIWLNEHADFNRVFVVGESAGANISYNVMGRASENEN 184
           PAAY+D W+A++W  +HSN +GP  W+N+HADF +VF+ G+SAG NIS+++  +A + + 
Sbjct: 122 PAAYEDVWSAIQWIFAHSNGSGPVDWINKHADFGKVFLGGDSAGGNISHHMAMKAGKEKK 181

Query: 185 GLNGMKVIGLGLVHPYFM-------IDKPDKLI---------EYVFPTSA-GLEDPRINP 244
               +K+ G+ +VHP F         D  DK           +   P S  G +DP  N 
Sbjct: 182 --LDLKIKGIAVVHPAFWGTDPVDEYDVQDKETRSGIAEIWEKIASPNSVNGTDDPLFNV 241

Query: 245 K-ANPGLGKLRCNRVLIFSAENDSLKYGAIGVHAAFKESGFEGSVEMVETEGEGHGFHLF 302
             +      L C++VL+  A  D      +   A  ++  +EG+VE+VE EGE H FHL 
Sbjct: 242 NGSGSDFSGLGCDKVLVAVAGKDVFVRQGLAYAAKLEKCEWEGTVEVVEEEGEDHVFHLQ 301

[Close]

BLAST of Lsi10G002950 vs. Swiss-Prot
Match: CXE13_ARATH (Probable carboxylesterase 13 OS=Arabidopsis thaliana GN=CXE13 PE=1 SV=1)

HSP 1 Score: 227.6 bits (579), Expect = 1.8e-58
Identity = 132/329 (40.12%), Postives = 202/329 (61.40%), Query Frame = 1


Query: 1   MDSQQEEIVFEFLPVFRVFKTGRIDRLILTDRVPPSTDQLTGVQSKDFLIDPQTGVSVRI 60
           MDS   EI  ++ P+  ++K+GRI+RL+    VPPS++   GV SKD +  P   +S+RI
Sbjct: 1   MDS---EIAADYSPMLIIYKSGRIERLVGETTVPPSSNPQNGVVSKDVVYSPDNNLSLRI 60

Query: 61  YLPQL-----SDSTNKLPLLIYIHGGAFVVGSPACLMFHNHLISLAAQANAVALSVEYRL 120
           YLP+      ++++ KLPLL+Y HGG F+V +     +H  L +  + ++ VA+SV+YR 
Sbjct: 61  YLPEKAATAETEASVKLPLLVYFHGGGFLVETAFSPTYHTFLTAAVSASDCVAVSVDYRR 120

Query: 121 APENNLPAAYDDAWTAVEWAASHSNHNGPEIWLNEHADFNRVFVVGESAGANISYNVMGR 180
           APE+ +P +YDD+WTA++W  SH   +G E WLN+HADF++VF+ G+SAGANI++++  +
Sbjct: 121 APEHPIPTSYDDSWTALKWVFSHIAGSGSEDWLNKHADFSKVFLAGDSAGANITHHMTMK 180

Query: 181 ASENE---NGLNGMKVIGLGLVHPYFMIDKP--DK---------LIEYVFPTSA-----G 240
           A++++     LN   + G+ LVHPYF    P  DK          IE V+  ++     G
Sbjct: 181 AAKDKLSPESLNESGISGIILVHPYFWSKTPVDDKETTDVAIRTWIESVWTLASPNSKDG 240

Query: 241 LEDPRIN--PKANPGLGKLRCNRVLIFSAENDSLKYGAIGVHAAFKESGFEGSV-EMVET 300
            +DP IN     +  L  L C +VL+  AE D+L     G      +S + G V ++VET
Sbjct: 241 SDDPFINVVQSESVDLSGLGCGKVLVMVAEKDALVRQGWGYWEKLGKSRWNGEVLDVVET 300

Query: 301 EGEGHGFHLFNPDTEKAHEVVKQLAFFIQ 303
           +GEGH FHL +P++EKAHE+V + A FI+
Sbjct: 301 KGEGHVFHLRDPNSEKAHELVHRFAGFIK 326

[Close]

BLAST of Lsi10G002950 vs. Swiss-Prot
Match: CXE12_ARATH (Probable carboxylesterase 12 OS=Arabidopsis thaliana GN=CXE12 PE=1 SV=1)

HSP 1 Score: 225.7 bits (574), Expect = 6.8e-58
Identity = 128/324 (39.51%), Postives = 195/324 (60.19%), Query Frame = 1


Query: 1   MDSQQEEIVFEFLPVFRVFKTGRIDRLILTDRVPPSTDQLTGVQSKDFLIDPQTGVSVRI 60
           MDS   EI  +  P+ +++K+GRI+RL+    VPPS++   GV SKD +      +SVRI
Sbjct: 1   MDS---EIAVDCSPLLKIYKSGRIERLMGEATVPPSSEPQNGVVSKDVVYSADNNLSVRI 60

Query: 61  YLPQ--LSDSTNKLPLLIYIHGGAFVVGSPACLMFHNHLISLAAQANAVALSVEYRLAPE 120
           YLP+   +++ +KLPLL+Y HGG F++ +     +H  L +  + +N VA+SV+YR APE
Sbjct: 61  YLPEKAAAETDSKLPLLVYFHGGGFIIETAFSPTYHTFLTTSVSASNCVAVSVDYRRAPE 120

Query: 121 NNLPAAYDDAWTAVEWAASHSNHNGPEIWLNEHADFNRVFVVGESAGANISYNVMGRASE 180
           + +   +DD+WTA++W  +H   +G E WLN+HADF+RVF+ G+SAGANI +++  RA++
Sbjct: 121 HPISVPFDDSWTALKWVFTHITGSGQEDWLNKHADFSRVFLSGDSAGANIVHHMAMRAAK 180

Query: 181 NE--NGLNGMKVIGLGLVHPYFM----IDKPDKLIE-----------YVFPTSA-GLEDP 240
            +   GLN   + G+ L+HPYF     ID+ D   E              P S  G +DP
Sbjct: 181 EKLSPGLNDTGISGIILLHPYFWSKTPIDEKDTKDETLRMKIEAFWMMASPNSKDGTDDP 240

Query: 241 RIN--PKANPGLGKLRCNRVLIFSAENDSLKYGAIGVHAAFKESGFEGSVEMVETEGEGH 300
            +N     +  L  L C +VL+  AE D+L     G  A  ++SG++G VE+VE+EGE H
Sbjct: 241 LLNVVQSESVDLSGLGCGKVLVMVAEKDALVRQGWGYAAKLEKSGWKGEVEVVESEGEDH 300

Query: 301 GFHLFNPDTEKAHEVVKQLAFFIQ 303
            FHL  P+ + A EV+ + + FI+
Sbjct: 301 VFHLLKPECDNAIEVMHKFSGFIK 321

[Close]

BLAST of Lsi10G002950 vs. TrEMBL
Match: Q0ZPW5_MALPM (CXE carboxylesterase OS=Malus pumila GN=CXE5 PE=2 SV=1)

HSP 1 Score: 334.0 bits (855), Expect = 2.0e-88
Identity = 156/302 (51.66%), Postives = 220/302 (72.85%), Query Frame = 1


Query: 1   MDSQ-QEEIVFEFLPVFRVFKTGRIDRLILTDRVPPSTDQLTGVQSKDFLIDPQTGVSVR 60
           MDS+   E+ FEF   FR++  GR +R    + VPPSTD  TGVQ KD ++ PQ+G+S R
Sbjct: 1   MDSKPSSEVSFEFPTAFRIYNDGRTERFKGIETVPPSTDSTTGVQCKDIVLSPQSGLSAR 60

Query: 61  IYLPQLSDSTNKLPLLIYIHGGAFVVGSPACLMFHNHLISLAAQANAVALSVEYRLAPEN 120
           ++LP+L D T KLPLLI+IHGGAFV+ SP   ++H H++ LA++AN VALSV YR APE+
Sbjct: 61  VFLPKLPDPTRKLPLLIFIHGGAFVIESPYSPLYHKHVMLLASEANVVALSVHYRRAPEH 120

Query: 121 NLPAAYDDAWTAVEWAASHSNHNGPEIWLNEHADFNRVFVVGESAGANISYNVMGRASEN 180
            LP A++D+W AVEWAA+HS  NGPE WLN+H DF+RVF+ G+SAGA ++++V+ +A   
Sbjct: 121 PLPVAFEDSWDAVEWAAAHSTRNGPEAWLNDHVDFDRVFIGGDSAGATLTHHVVRQA--G 180

Query: 181 ENGLNGMKVIGLGLVHPYFMIDKPDKLIEYVFPTSAGLEDPRINPKANPGLGKLRCNRVL 240
            +GL+G +++G+ L HPYFM D+PDKL+E ++PT  G +DPR+ P  +P LG++ C RVL
Sbjct: 181 LDGLSGTRIVGMILFHPYFMDDEPDKLLEVIYPTCGGSDDPRVRPGNDPKLGEIGCGRVL 240

Query: 241 IFSAENDSLKYGAIGVHAAFKESGFEGSVEMVETEGEGHGFHLFNPDTEKAHEVVKQLAF 300
           +F AE D L+      H A K+SG+ G VE+VE++GE H FHLFNP  + A ++VK++  
Sbjct: 241 VFVAEKDFLRDRGWAYHEALKKSGYGGVVEIVESQGEDHVFHLFNPSCDNAVDLVKKVVS 300

Query: 301 FI 302
           F+
Sbjct: 301 FV 300

[Close]

BLAST of Lsi10G002950 vs. TrEMBL
Match: M5VQ13_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa009160mg PE=4 SV=1)

HSP 1 Score: 325.9 bits (834), Expect = 5.4e-86
Identity = 155/299 (51.84%), Postives = 219/299 (73.24%), Query Frame = 1


Query: 3   SQQEEIVFEFLPVFRVFKTGRIDRLILTDRVPPSTDQLTGVQSKDFLIDPQTGVSVRIYL 62
           S   EI  EF  +FR++K GR++RL  T+ VPPSTD  TGV+SKD ++  ++G+S RI+L
Sbjct: 5   SSSTEISVEFPTIFRIYKDGRVERLKGTETVPPSTDPKTGVRSKDIVLSHESGLSARIFL 64

Query: 63  PQLSDSTNKLPLLIYIHGGAFVVGSPACLMFHNHLISLAAQANAVALSVEYRLAPENNLP 122
           P+L+D T KLPLL++IHGGAFV+ SP   ++HNH   L ++AN V LSV YR APE+ LP
Sbjct: 65  PKLTDPTRKLPLLVFIHGGAFVIESPYSPLYHNHAALLTSEANVVVLSVNYRRAPEHPLP 124

Query: 123 AAYDDAWTAVEWAASHSNHNGPEIWLNEHADFNRVFVVGESAGANISYNVMGRASENENG 182
            AY+D+W A++WAA+HSN NGPE WLN++ +F+RVFV G+SAGA ++++V+ RA    +G
Sbjct: 125 VAYEDSWEALQWAAAHSNRNGPEAWLNDYVNFDRVFVGGDSAGATLTHHVVHRA--GVDG 184

Query: 183 LNGMKVIGLGLVHPYFMIDKPDKLIEYVFPTSAGLEDPRINPKANPGLGKLRCNRVLIFS 242
           L+G K++G+ L HP+F   K DKL+E +FP   GL+DPR+NP  +P LGK+ C RVL+F 
Sbjct: 185 LSGAKIVGMILFHPFFGNGKLDKLLEVIFPECNGLDDPRVNPGKDPKLGKIGCRRVLVFV 244

Query: 243 AENDSLKYGAIGVHAAFKESGFEGSVEMVETEGEGHGFHLFNPDTEKAHEVVKQLAFFI 302
           AE D L+  A   + + K+SG+ G VE++E+EGE H FHLFNP  +KA  +V+++  FI
Sbjct: 245 AEKDFLRDRAWAYYESLKKSGWGGVVEILESEGEDHVFHLFNPSCDKAVALVQKVVSFI 301

[Close]

BLAST of Lsi10G002950 vs. TrEMBL
Match: A0A061E1M8_THECC (Alpha/beta-Hydrolases superfamily protein, putative OS=Theobroma cacao GN=TCM_007027 PE=4 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 2.1e-82
Identity = 151/305 (49.51%), Postives = 225/305 (73.77%), Query Frame = 1


Query: 1   MDSQQEEIVFEFL---PVFRVFKTGRIDRLILTDRVPPSTDQLTGVQSKDFLIDPQTGVS 60
           M S   EI  EF+   P+FRV+K GRI+RL  T+ VPPS D  TGVQSKD ++ P++ +S
Sbjct: 1   MASNTTEIAREFVNSFPLFRVYKDGRIERLRETETVPPSNDPQTGVQSKDTIVLPESSLS 60

Query: 61  VRIYLPQLSDSTNKLPLLIYIHGGAFVVGSPACLMFHNHLISLAAQANAVALSVEYRLAP 120
            R++LP+++D T K+PL+IYIHGGAF + SP   ++HN+L SL  +AN +A++++YR AP
Sbjct: 61  ARLFLPKITDPTAKIPLVIYIHGGAFCIESPFSPLYHNYLTSLVNKANVIAVAIQYRKAP 120

Query: 121 ENNLPAAYDDAWTAVEWAASHSNHNGPEIWLNEHADFNRVFVVGESAGANISYNVMGRAS 180
           E  LP AYDDAWTA++W ASH+N +GPE WLN+HADF RVF  G+SAGANI++N+  +A 
Sbjct: 121 EYPLPIAYDDAWTAIKWVASHANRDGPEPWLNDHADFERVFFAGDSAGANIAHNMTMKA- 180

Query: 181 ENENGLNGMKVIGLGLVHPYFMIDKPDKLIEYVFPTSAGLEDPRINP-KANPGLGKLRCN 240
              +GL G+K++G+ L++P+F  ++PD+LIEY+FP+S+G  DPR+NP  A   L  L C+
Sbjct: 181 -GADGLIGVKLVGMLLMNPFFRNNEPDELIEYIFPSSSGCNDPRMNPASAIKELAGLACS 240

Query: 241 RVLIFSAENDSLKYGAIGVHAAFKESGFEGSVEMVETEGEGHGFHLFNPDTEKAHEVVKQ 300
           RVL+  +E D LK   +  + A ++SG++G +++VET GE H F+LF+P ++KA +++ Q
Sbjct: 241 RVLVCVSEKDFLKDRGVTYYEAVRKSGWDGVIDIVETPGEKHVFYLFDPSSQKAMDLMDQ 300

Query: 301 LAFFI 302
           ++ F+
Sbjct: 301 VSSFL 303

[Close]

BLAST of Lsi10G002950 vs. TrEMBL
Match: W9SMM8_9ROSA (Putative carboxylesterase 7 OS=Morus notabilis GN=L484_027556 PE=4 SV=1)

HSP 1 Score: 313.2 bits (801), Expect = 3.6e-82
Identity = 156/302 (51.66%), Postives = 213/302 (70.53%), Query Frame = 1


Query: 1   MDSQQEEIVFEFLPVFRVFKTGRIDRLILTDRVPPSTDQLTGVQSKDFLIDPQTGVSVRI 60
           M+ + +E+  EF P FR++K GRIDR    + VPPS D  T  +SKD +I P++G+S R+
Sbjct: 1   MEQRSDEVAIEF-PFFRIYKDGRIDRFFGDEIVPPSPDSATAARSKDVVISPESGLSARL 60

Query: 61  YLPQLSDSTNKLPLLIYIHGGAFVVGSPACLMFHNHLISLAAQANAVALSVEYRLAPENN 120
           ++ Q+ D T KLPLL+Y+HGGAF VG+P    +  H  SLAA++NA+ LSV YR APE+ 
Sbjct: 61  FIHQIPDPTRKLPLLVYLHGGAFCVGTPFSPTYTGHASSLAAESNAIVLSVHYRRAPEHP 120

Query: 121 LPAAYDDAWTAVEWAASHS-NHNGPEIWLNEHADFNRVFVVGESAGANISYNVMGRASEN 180
           LP AYDDAW A++WA  HS    GPE WLN+HADF+RVFV G+SAGANI++ V+ RA   
Sbjct: 121 LPIAYDDAWEALQWAVVHSGGGGGPEPWLNQHADFDRVFVGGDSAGANIAHYVVLRA--G 180

Query: 181 ENGLNGMKVIGLGLVHPYFMIDKPDKLIEYVFPTSAGLEDPRINPKANPGLGKLRCNRVL 240
            +GLNG ++ GL LVHP+F  DKP++L+E +FP+ +   DPR+NP  +P L +L C RVL
Sbjct: 181 VDGLNGPRIAGLVLVHPFFGNDKPNRLLEIIFPSRSVPNDPRVNPGFDPDLSRLGCGRVL 240

Query: 241 IFSAENDSLKYGAIGVHAAFKESGFEGSVEMVETEGEGHGFHLFNPDTEKAHEVVKQLAF 300
           IF AE D LK  A+  + A K+SG+ G VE+VE EG  H FHLF+P  E+A  +VK+++ 
Sbjct: 241 IFVAEKDFLKDRALAYYEALKKSGWIGLVELVEYEGVDHVFHLFSPKCEQAQALVKKVSS 299

Query: 301 FI 302
           F+
Sbjct: 301 FL 299

[Close]

BLAST of Lsi10G002950 vs. TrEMBL
Match: F6HQD1_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_03s0063g00750 PE=4 SV=1)

HSP 1 Score: 308.9 bits (790), Expect = 6.8e-81
Identity = 154/316 (48.73%), Postives = 208/316 (65.82%), Query Frame = 1


Query: 1   MDSQQE-EIVFEFLPVFRVFKTGRIDRLILTDRVPPSTDQLTGVQSKDFLIDPQTGVSVR 60
           MDS    E+ F F P  RVFK G+++R + TD VPPS +  TGV SKD +IDP+TGVS R
Sbjct: 1   MDSNTNPELSFNFPPFLRVFKDGKVERFVGTDSVPPSLNIETGVNSKDIVIDPETGVSAR 60

Query: 61  IYLPQLSDSTNKLPLLIYIHGGAFVVGSPACLMFHNHLISLAAQANAVALSVEYRLAPEN 120
           +Y+P+++D + KLPLL+Y HGGAF + + +   +HN+L SL A+AN VA+S+EYR APE+
Sbjct: 61  LYIPKINDQSQKLPLLVYFHGGAFCIETFSSPTYHNYLDSLVAEANVVAVSIEYRRAPEH 120

Query: 121 NLPAAYDDAWTAVEWAASHSNHNGPEIWLNEHADFNRVFVVGESAGANISYNVMGRASEN 180
            LP AYDD W AV+W  SHSN  GPE WLN++AD +R+F  G+SAGAN+S+N+  RA   
Sbjct: 121 PLPVAYDDCWAAVKWLVSHSNSQGPEPWLNDYADLDRLFFAGDSAGANLSHNMAIRAGTR 180

Query: 181 ENGLNGMKVIGLGLVHPYFMIDKP--------------DKLIEYVFPTSAGLEDPRINPK 240
            + L  +KV G+ L+HPYF    P              D L  +V PT++G +DP INP 
Sbjct: 181 GHELGSVKVSGIILIHPYFWGKDPVGAEVKDLQKKGLVDSLWLFVCPTTSGCDDPLINPA 240

Query: 241 ANPGLGKLRCNRVLIFSAENDSLKYGAIGVHAAFKESGFEGSVEMVETEGEGHGFHLFNP 300
            +P L  L C RVL+F AE D+L+      H    +SG+ G VE++E EGE H FHLFNP
Sbjct: 241 TDPKLASLGCQRVLVFVAEKDTLRDRGWFYHETLGKSGWSGVVEVMEAEGEDHVFHLFNP 300

Query: 301 DTEKAHEVVKQLAFFI 302
             +KA  ++KQ+A F+
Sbjct: 301 TCDKAVAMLKQMAMFL 316

[Close]

BLAST of Lsi10G002950 vs. TAIR10
Match: AT1G47480.1 (AT1G47480.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 235.3 bits (599), Expect = 4.9e-62
Identity = 128/317 (40.38%), Postives = 194/317 (61.20%), Query Frame = 1


Query: 1   MDSQQEEIVFEFLPVFRVFKTGRIDRLILTDRVPPSTDQLTGVQSKDFLIDPQTGVSVRI 60
           M+S ++++  E LP   V   G ++RL  T+  PP  D +TGV SKD +I+P+TG+S RI
Sbjct: 1   MESTKKQVSLELLPWLVVHTDGTVERLAGTEVCPPGLDPITGVFSKDIIIEPKTGLSARI 60

Query: 61  YLPQLSDSTNKLPLLIYIHGGAFVVGSPACLMFHNHLISLAAQANAVALSVEYRLAPENN 120
           Y P       K+PL++Y HGGAF++ S +   +H  L  +  QAN +A+SV YRLAPE+ 
Sbjct: 61  YRPFSIQPGQKIPLMLYFHGGAFLISSTSFPSYHTSLNKIVNQANVIAVSVNYRLAPEHP 120

Query: 121 LPAAYDDAWTAVEWAASHSNHNGPEIWLNEHADFNRVFVVGESAGANISYNVMGRASENE 180
           LP AY+D+WTA++   + +     E W+N++AD + +F+VG+SAGANIS+++  RA +++
Sbjct: 121 LPTAYEDSWTALKNIQAIN-----EPWINDYADLDSLFLVGDSAGANISHHLAFRAKQSD 180

Query: 181 NGLNGMKVIGLGLVHPYFMIDKP--------------DKLIEYVFPTSAGLEDPRINPKA 240
             L   K+ G+G++HPYF   +P              D   E+V P+  G +DP INP A
Sbjct: 181 QTL---KIKGIGMIHPYFWGTQPIGAEIKDEARKQMVDGWWEFVCPSEKGSDDPWINPFA 240

Query: 241 --NPGLGKLRCNRVLIFSAENDSLKYGAIGVHAAFKESGFEGSVEMVETEGEGHGFHLFN 300
             +P LG L C RV+I  AE D L       +    +S ++G VE++ET+ + H FH+F 
Sbjct: 241 DGSPDLGGLGCERVMITVAEKDILNERGKMYYERLVKSEWKGKVEIMETKEKDHVFHIFE 300

Query: 301 PDTEKAHEVVKQLAFFI 302
           PD ++A E+V+ LA FI
Sbjct: 301 PDCDEAMEMVRCLALFI 309

[Close]

BLAST of Lsi10G002950 vs. TAIR10
Match: AT1G19190.1 (AT1G19190.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 234.6 bits (597), Expect = 8.3e-62
Identity = 128/318 (40.25%), Postives = 190/318 (59.75%), Query Frame = 1


Query: 1   MDSQQEEIVFEFLPVFRVFKTGRIDRLILTDRVPPSTDQLTGVQSKDFLIDPQTGVSVRI 60
           MDS   EI F++ P FR+FK G I+RL+    VPPS +   GV SKD +  P+  +S+RI
Sbjct: 1   MDS---EIAFDYSPRFRIFKNGGIERLVPETFVPPSLNPENGVVSKDAVYSPEKNLSLRI 60

Query: 61  YLPQLS---DSTNKLPLLIYIHGGAFVVGSPACLMFHNHLISLAAQANAVALSVEYRLAP 120
           YLPQ S       K+PLL+Y HGG F++ +    ++H  L S  +  + +A+SVEYR AP
Sbjct: 61  YLPQNSVYETGEKKIPLLVYFHGGGFIMETAFSPIYHTFLTSAVSATDCIAVSVEYRRAP 120

Query: 121 ENNLPAAYDDAWTAVEWAASHSNHNGPEIWLNEHADFNRVFVVGESAGANISYNVMGRAS 180
           E+ +P  Y+D+W A++W  +H   +GPE WLN+HADF++VF+ G+SAGANI++++  R  
Sbjct: 121 EHPIPTLYEDSWDAIQWIFTHITRSGPEDWLNKHADFSKVFLAGDSAGANIAHHMAIRVD 180

Query: 181 ENENGLNGMKVIGLGLVHPYFM------------IDKPDKLIEYVFPTSA-GLEDPRINP 240
           + +      K+ G+ L HPYF+            +   ++L     P S  G+EDP IN 
Sbjct: 181 KEKLPPENFKISGMILFHPYFLSKALIEEMEVEAMRYYERLWRIASPDSGNGVEDPWINV 240

Query: 241 KANPGLGKLRCNRVLIFSAENDSLKYGAIGVHAAFKESGFEGSVEMVETEGEGHGFHLFN 300
             +   G L C RVL+  A ND L  G     A  ++SG+ G V+++ET+ EGH FHL +
Sbjct: 241 VGSDLTG-LGCRRVLVMVAGNDVLARGGWSYVAELEKSGWIGKVKVMETKEEGHVFHLRD 300

Query: 301 PDTEKAHEVVKQLAFFIQ 303
           PD+E A  V++  A F++
Sbjct: 301 PDSENARRVLRNFAEFLK 314

[Close]

BLAST of Lsi10G002950 vs. TAIR10
Match: AT1G49660.1 (AT1G49660.1 carboxyesterase 5)

HSP 1 Score: 232.6 bits (592), Expect = 3.1e-61
Identity = 128/318 (40.25%), Postives = 189/318 (59.43%), Query Frame = 1


Query: 5   QEEIVFEFLPVFRVFKTGRIDRLILTDRVPPSTDQLTGVQSKDFLIDPQTGVSVRIYLPQ 64
           + EI  EFLP  R++K GR++RLI TD +P S D    V SKD +  P+  +SVR++LP 
Sbjct: 2   ESEIASEFLPFCRIYKDGRVERLIGTDTIPASLDPTYDVVSKDVIYSPENNLSVRLFLPH 61

Query: 65  LSDST---NKLPLLIYIHGGAFVVGSPACLMFHNHLISLAAQANAVALSVEYRLAPENNL 124
            S      NKLPLLIYIHGGA+++ SP   ++HN+L  +   AN +A+SV+YR APE+ +
Sbjct: 62  KSTKLTAGNKLPLLIYIHGGAWIIESPFSPLYHNYLTEVVKSANCLAVSVQYRRAPEDPV 121

Query: 125 PAAYDDAWTAVEWAASHSNHNGPEIWLNEHADFNRVFVVGESAGANISYNVMGRASENEN 184
           PAAY+D W+A++W  +HSN +GP  W+N+HADF +VF+ G+SAG NIS+++  +A + + 
Sbjct: 122 PAAYEDVWSAIQWIFAHSNGSGPVDWINKHADFGKVFLGGDSAGGNISHHMAMKAGKEKK 181

Query: 185 GLNGMKVIGLGLVHPYFM-------IDKPDKLI---------EYVFPTSA-GLEDPRINP 244
               +K+ G+ +VHP F         D  DK           +   P S  G +DP  N 
Sbjct: 182 --LDLKIKGIAVVHPAFWGTDPVDEYDVQDKETRSGIAEIWEKIASPNSVNGTDDPLFNV 241

Query: 245 K-ANPGLGKLRCNRVLIFSAENDSLKYGAIGVHAAFKESGFEGSVEMVETEGEGHGFHLF 302
             +      L C++VL+  A  D      +   A  ++  +EG+VE+VE EGE H FHL 
Sbjct: 242 NGSGSDFSGLGCDKVLVAVAGKDVFVRQGLAYAAKLEKCEWEGTVEVVEEEGEDHVFHLQ 301

[Close]

BLAST of Lsi10G002950 vs. TAIR10
Match: AT3G48700.1 (AT3G48700.1 carboxyesterase 13)

HSP 1 Score: 227.6 bits (579), Expect = 1.0e-59
Identity = 132/329 (40.12%), Postives = 202/329 (61.40%), Query Frame = 1


Query: 1   MDSQQEEIVFEFLPVFRVFKTGRIDRLILTDRVPPSTDQLTGVQSKDFLIDPQTGVSVRI 60
           MDS   EI  ++ P+  ++K+GRI+RL+    VPPS++   GV SKD +  P   +S+RI
Sbjct: 1   MDS---EIAADYSPMLIIYKSGRIERLVGETTVPPSSNPQNGVVSKDVVYSPDNNLSLRI 60

Query: 61  YLPQL-----SDSTNKLPLLIYIHGGAFVVGSPACLMFHNHLISLAAQANAVALSVEYRL 120
           YLP+      ++++ KLPLL+Y HGG F+V +     +H  L +  + ++ VA+SV+YR 
Sbjct: 61  YLPEKAATAETEASVKLPLLVYFHGGGFLVETAFSPTYHTFLTAAVSASDCVAVSVDYRR 120

Query: 121 APENNLPAAYDDAWTAVEWAASHSNHNGPEIWLNEHADFNRVFVVGESAGANISYNVMGR 180
           APE+ +P +YDD+WTA++W  SH   +G E WLN+HADF++VF+ G+SAGANI++++  +
Sbjct: 121 APEHPIPTSYDDSWTALKWVFSHIAGSGSEDWLNKHADFSKVFLAGDSAGANITHHMTMK 180

Query: 181 ASENE---NGLNGMKVIGLGLVHPYFMIDKP--DK---------LIEYVFPTSA-----G 240
           A++++     LN   + G+ LVHPYF    P  DK          IE V+  ++     G
Sbjct: 181 AAKDKLSPESLNESGISGIILVHPYFWSKTPVDDKETTDVAIRTWIESVWTLASPNSKDG 240

Query: 241 LEDPRIN--PKANPGLGKLRCNRVLIFSAENDSLKYGAIGVHAAFKESGFEGSV-EMVET 300
            +DP IN     +  L  L C +VL+  AE D+L     G      +S + G V ++VET
Sbjct: 241 SDDPFINVVQSESVDLSGLGCGKVLVMVAEKDALVRQGWGYWEKLGKSRWNGEVLDVVET 300

Query: 301 EGEGHGFHLFNPDTEKAHEVVKQLAFFIQ 303
           +GEGH FHL +P++EKAHE+V + A FI+
Sbjct: 301 KGEGHVFHLRDPNSEKAHELVHRFAGFIK 326

[Close]

BLAST of Lsi10G002950 vs. TAIR10
Match: AT3G48690.1 (AT3G48690.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 225.7 bits (574), Expect = 3.8e-59
Identity = 128/324 (39.51%), Postives = 195/324 (60.19%), Query Frame = 1


Query: 1   MDSQQEEIVFEFLPVFRVFKTGRIDRLILTDRVPPSTDQLTGVQSKDFLIDPQTGVSVRI 60
           MDS   EI  +  P+ +++K+GRI+RL+    VPPS++   GV SKD +      +SVRI
Sbjct: 1   MDS---EIAVDCSPLLKIYKSGRIERLMGEATVPPSSEPQNGVVSKDVVYSADNNLSVRI 60

Query: 61  YLPQ--LSDSTNKLPLLIYIHGGAFVVGSPACLMFHNHLISLAAQANAVALSVEYRLAPE 120
           YLP+   +++ +KLPLL+Y HGG F++ +     +H  L +  + +N VA+SV+YR APE
Sbjct: 61  YLPEKAAAETDSKLPLLVYFHGGGFIIETAFSPTYHTFLTTSVSASNCVAVSVDYRRAPE 120

Query: 121 NNLPAAYDDAWTAVEWAASHSNHNGPEIWLNEHADFNRVFVVGESAGANISYNVMGRASE 180
           + +   +DD+WTA++W  +H   +G E WLN+HADF+RVF+ G+SAGANI +++  RA++
Sbjct: 121 HPISVPFDDSWTALKWVFTHITGSGQEDWLNKHADFSRVFLSGDSAGANIVHHMAMRAAK 180

Query: 181 NE--NGLNGMKVIGLGLVHPYFM----IDKPDKLIE-----------YVFPTSA-GLEDP 240
            +   GLN   + G+ L+HPYF     ID+ D   E              P S  G +DP
Sbjct: 181 EKLSPGLNDTGISGIILLHPYFWSKTPIDEKDTKDETLRMKIEAFWMMASPNSKDGTDDP 240

Query: 241 RIN--PKANPGLGKLRCNRVLIFSAENDSLKYGAIGVHAAFKESGFEGSVEMVETEGEGH 300
            +N     +  L  L C +VL+  AE D+L     G  A  ++SG++G VE+VE+EGE H
Sbjct: 241 LLNVVQSESVDLSGLGCGKVLVMVAEKDALVRQGWGYAAKLEKSGWKGEVEVVESEGEDH 300

Query: 301 GFHLFNPDTEKAHEVVKQLAFFIQ 303
            FHL  P+ + A EV+ + + FI+
Sbjct: 301 VFHLLKPECDNAIEVMHKFSGFIK 321

[Close]

BLAST of Lsi10G002950 vs. NCBI nr
Match: gi|470117387|ref|XP_004294840.1| (PREDICTED: probable carboxylesterase 7 [Fragaria vesca subsp. vesca])

HSP 1 Score: 339.3 bits (869), Expect = 6.8e-90
Identity = 162/299 (54.18%), Postives = 219/299 (73.24%), Query Frame = 1


Query: 3   SQQEEIVFEFLPVFRVFKTGRIDRLILTDRVPPSTDQLTGVQSKDFLIDPQTGVSVRIYL 62
           +Q +EI FEF P+FR++K GR DRL   + VPPSTD  TGVQSKD ++ P++G+S RIYL
Sbjct: 5   TQNDEIAFEFPPLFRLYKDGRADRLKGNETVPPSTDPTTGVQSKDIVLSPESGLSARIYL 64

Query: 63  PQLSDSTNKLPLLIYIHGGAFVVGSPACLMFHNHLISLAAQANAVALSVEYRLAPENNLP 122
           P+L D T KLP L+YIHGG FV+ SP+  ++H+H+ SLAAQAN VALS+ YR APE+ LP
Sbjct: 65  PKLPDPTRKLPFLLYIHGGGFVIESPSSPVYHSHVSSLAAQANVVALSIHYRRAPEHPLP 124

Query: 123 AAYDDAWTAVEWAASHSNHNGPEIWLNEHADFNRVFVVGESAGANISYNVMGRASENENG 182
           AA++D+W A +WAA+HS   GPE WLNEHADFNRVFV G+SAG+ ++++V+ ++     G
Sbjct: 125 AAFEDSWAAFQWAATHSTGGGPESWLNEHADFNRVFVAGDSAGSTLAHHVVRQS--GVEG 184

Query: 183 LNGMKVIGLGLVHPYFMIDKPDKLIEYVFPTSAGLEDPRINPKANPGLGKLRCNRVLIFS 242
           ++G+  +G+ L HPYF  + PDKL+E ++PT  GLEDP++ P  +P LG L   RVL+F 
Sbjct: 185 VSGVNTVGVVLFHPYFRNEVPDKLLEVIYPTGGGLEDPKMYPGNDPRLGSLGAGRVLVFV 244

Query: 243 AENDSLKYGAIGVHAAFKESGFEGSVEMVETEGEGHGFHLFNPDTEKAHEVVKQLAFFI 302
           AE D LK      + A K+SG+ G VE+ ETEGE H FHLF+P  EKA  +VK++A F+
Sbjct: 245 AEKDFLKDRGWSYYEALKKSGWSGVVEIEETEGEDHVFHLFDPSCEKAVSLVKKVAAFM 301

[Close]

BLAST of Lsi10G002950 vs. NCBI nr
Match: gi|694353183|ref|XP_009358071.1| (PREDICTED: probable carboxylesterase 12 [Pyrus x bretschneideri])

HSP 1 Score: 339.0 bits (868), Expect = 8.8e-90
Identity = 160/302 (52.98%), Postives = 224/302 (74.17%), Query Frame = 1


Query: 1   MDSQ-QEEIVFEFLPVFRVFKTGRIDRLILTDRVPPSTDQLTGVQSKDFLIDPQTGVSVR 60
           MDS+   E+ FEF  VFR++  GR +RL  T+ VPPSTD  TGVQSKD ++ PQ+G+S R
Sbjct: 1   MDSKPSSEVSFEFPTVFRIYNDGRTERLKGTETVPPSTDPTTGVQSKDIVLSPQSGLSAR 60

Query: 61  IYLPQLSDSTNKLPLLIYIHGGAFVVGSPACLMFHNHLISLAAQANAVALSVEYRLAPEN 120
           ++LP+L D T KLPLLI+IHGGAFV+ SP   ++HNH+  LA++AN VALSV YR APE+
Sbjct: 61  VFLPKLPDPTRKLPLLIFIHGGAFVIESPYSPLYHNHVGLLASEANVVALSVHYRRAPEH 120

Query: 121 NLPAAYDDAWTAVEWAASHSNHNGPEIWLNEHADFNRVFVVGESAGANISYNVMGRASEN 180
            LP A++D+W AV+WAA+HS+ NGPE WLN+H DF+RVF+ G+SAGA +++NV+ +A   
Sbjct: 121 PLPVAFEDSWDAVQWAAAHSSGNGPEAWLNDHVDFDRVFIGGDSAGATLTHNVVRQA--G 180

Query: 181 ENGLNGMKVIGLGLVHPYFMIDKPDKLIEYVFPTSAGLEDPRINPKANPGLGKLRCNRVL 240
            +GL G +++G+ L HPYFM D+PDKL+E ++PT  G +DPR+ P  +P LG++ C RVL
Sbjct: 181 LDGLTGARIVGMILFHPYFMDDEPDKLLEVIYPTCGGSDDPRVRPGNDPKLGEIGCGRVL 240

Query: 241 IFSAENDSLKYGAIGVHAAFKESGFEGSVEMVETEGEGHGFHLFNPDTEKAHEVVKQLAF 300
           +F AE D L+      + A K+SG+ G VE+VE+EGE H FHLFNP  + + ++VK++  
Sbjct: 241 VFVAEKDFLRDRGWAYYEALKKSGYGGVVEIVESEGEDHVFHLFNPSCDNSVDLVKKVVS 300

Query: 301 FI 302
           F+
Sbjct: 301 FV 300

[Close]

BLAST of Lsi10G002950 vs. NCBI nr
Match: gi|694450813|ref|XP_009350720.1| (PREDICTED: probable carboxylesterase 12 [Pyrus x bretschneideri])

HSP 1 Score: 334.3 bits (856), Expect = 2.2e-88
Identity = 159/302 (52.65%), Postives = 222/302 (73.51%), Query Frame = 1


Query: 1   MDSQ-QEEIVFEFLPVFRVFKTGRIDRLILTDRVPPSTDQLTGVQSKDFLIDPQTGVSVR 60
           MDS+   E+ FEF  VFR++  GR +RL  T+ VPPSTD  TGVQSKD ++ PQ+G+S R
Sbjct: 1   MDSKPSSEVSFEFPTVFRIYNDGRTERLKGTETVPPSTDPTTGVQSKDIVLSPQSGLSAR 60

Query: 61  IYLPQLSDSTNKLPLLIYIHGGAFVVGSPACLMFHNHLISLAAQANAVALSVEYRLAPEN 120
           ++LP+L D T KLPLLI+IHGGAFV+ SP   ++HNH+  LA++AN VALSV YR APE+
Sbjct: 61  VFLPKLPDPTRKLPLLIFIHGGAFVIESPYSPLYHNHVGFLASEANVVALSVHYRRAPEH 120

Query: 121 NLPAAYDDAWTAVEWAASHSNHNGPEIWLNEHADFNRVFVVGESAGANISYNVMGRASEN 180
            LP A++D+W AV+WAA+HS+ NGPE WLN+H DF+RVF+ G+SAGA +++NV+ +A   
Sbjct: 121 PLPVAFEDSWDAVQWAAAHSSGNGPEAWLNDHVDFDRVFIGGDSAGATLTHNVVRQA--G 180

Query: 181 ENGLNGMKVIGLGLVHPYFMIDKPDKLIEYVFPTSAGLEDPRINPKANPGLGKLRCNRVL 240
            +GL G +++G+ L HPYFM D+PDKL+E ++PT  G +DPR+ P  +  LG++ C RVL
Sbjct: 181 LDGLTGARIVGMILFHPYFMDDEPDKLLEVIYPTCGGSDDPRVRPGNDLKLGEIGCGRVL 240

Query: 241 IFSAENDSLKYGAIGVHAAFKESGFEGSVEMVETEGEGHGFHLFNPDTEKAHEVVKQLAF 300
           +F AE D L+      + A K+SG+ G VE+VE+EGE H FHLFNP  + +  +VK++  
Sbjct: 241 VFVAEKDFLRDRGWAYYEALKKSGYGGVVEIVESEGEDHVFHLFNPSCDNSVNLVKKVVS 300

Query: 301 FI 302
           F+
Sbjct: 301 FV 300

[Close]

BLAST of Lsi10G002950 vs. NCBI nr
Match: gi|82697941|gb|ABB89005.1| (CXE carboxylesterase [Malus pumila])

HSP 1 Score: 334.0 bits (855), Expect = 2.8e-88
Identity = 156/302 (51.66%), Postives = 220/302 (72.85%), Query Frame = 1


Query: 1   MDSQ-QEEIVFEFLPVFRVFKTGRIDRLILTDRVPPSTDQLTGVQSKDFLIDPQTGVSVR 60
           MDS+   E+ FEF   FR++  GR +R    + VPPSTD  TGVQ KD ++ PQ+G+S R
Sbjct: 1   MDSKPSSEVSFEFPTAFRIYNDGRTERFKGIETVPPSTDSTTGVQCKDIVLSPQSGLSAR 60

Query: 61  IYLPQLSDSTNKLPLLIYIHGGAFVVGSPACLMFHNHLISLAAQANAVALSVEYRLAPEN 120
           ++LP+L D T KLPLLI+IHGGAFV+ SP   ++H H++ LA++AN VALSV YR APE+
Sbjct: 61  VFLPKLPDPTRKLPLLIFIHGGAFVIESPYSPLYHKHVMLLASEANVVALSVHYRRAPEH 120

Query: 121 NLPAAYDDAWTAVEWAASHSNHNGPEIWLNEHADFNRVFVVGESAGANISYNVMGRASEN 180
            LP A++D+W AVEWAA+HS  NGPE WLN+H DF+RVF+ G+SAGA ++++V+ +A   
Sbjct: 121 PLPVAFEDSWDAVEWAAAHSTRNGPEAWLNDHVDFDRVFIGGDSAGATLTHHVVRQA--G 180

Query: 181 ENGLNGMKVIGLGLVHPYFMIDKPDKLIEYVFPTSAGLEDPRINPKANPGLGKLRCNRVL 240
            +GL+G +++G+ L HPYFM D+PDKL+E ++PT  G +DPR+ P  +P LG++ C RVL
Sbjct: 181 LDGLSGTRIVGMILFHPYFMDDEPDKLLEVIYPTCGGSDDPRVRPGNDPKLGEIGCGRVL 240

Query: 241 IFSAENDSLKYGAIGVHAAFKESGFEGSVEMVETEGEGHGFHLFNPDTEKAHEVVKQLAF 300
           +F AE D L+      H A K+SG+ G VE+VE++GE H FHLFNP  + A ++VK++  
Sbjct: 241 VFVAEKDFLRDRGWAYHEALKKSGYGGVVEIVESQGEDHVFHLFNPSCDNAVDLVKKVVS 300

Query: 301 FI 302
           F+
Sbjct: 301 FV 300

[Close]

BLAST of Lsi10G002950 vs. NCBI nr
Match: gi|657999750|ref|XP_008392308.1| (PREDICTED: probable carboxylesterase 7 [Malus domestica])

HSP 1 Score: 332.4 bits (851), Expect = 8.3e-88
Identity = 156/302 (51.66%), Postives = 219/302 (72.52%), Query Frame = 1


Query: 1   MDSQ-QEEIVFEFLPVFRVFKTGRIDRLILTDRVPPSTDQLTGVQSKDFLIDPQTGVSVR 60
           MDS+   E+ FEF   FR++  GR +R    + VPPSTD  TGVQ KD ++ PQ+G+S R
Sbjct: 59  MDSKPSSEVSFEFPTAFRIYNDGRTERFKGIETVPPSTDSTTGVQCKDIVLSPQSGLSAR 118

Query: 61  IYLPQLSDSTNKLPLLIYIHGGAFVVGSPACLMFHNHLISLAAQANAVALSVEYRLAPEN 120
           ++LP+L D T KLPLLI+IHGGAFV+ SP   ++H H+  LA++AN VALSV YR APE+
Sbjct: 119 VFLPKLPDPTRKLPLLIFIHGGAFVIESPYSPLYHKHVRLLASEANVVALSVHYRRAPEH 178

Query: 121 NLPAAYDDAWTAVEWAASHSNHNGPEIWLNEHADFNRVFVVGESAGANISYNVMGRASEN 180
            LP A++D+W AVEWAA+HS  NGPE WLN+H DF+RVF+ G+SAGA ++++V+ +A   
Sbjct: 179 PLPVAFEDSWDAVEWAAAHSTRNGPEAWLNDHVDFDRVFIGGDSAGATLTHHVVRQA--G 238

Query: 181 ENGLNGMKVIGLGLVHPYFMIDKPDKLIEYVFPTSAGLEDPRINPKANPGLGKLRCNRVL 240
            +GL+G +++G+ L HPYFM D+PDKL+E ++PT  G +DPR+ P  +P LG++ C RVL
Sbjct: 239 LDGLSGTRIVGMILFHPYFMDDEPDKLLEVIYPTCGGSDDPRVRPGNDPKLGEIGCGRVL 298

Query: 241 IFSAENDSLKYGAIGVHAAFKESGFEGSVEMVETEGEGHGFHLFNPDTEKAHEVVKQLAF 300
           +F AE D L+      H A K+SG+ G VE+VE++GE H FHLFNP  + A ++VK++  
Sbjct: 299 VFVAEKDFLRDRGWAYHEALKKSGYGGVVEIVESQGEDHVFHLFNPSCDNAVDLVKKVVS 358

Query: 301 FI 302
           F+
Sbjct: 359 FV 358

The following BLAST results are available for this feature:

BLAST of Lsi10G002950 vs. Swiss-Prot


Match Name	E-value	Identity	Description
CXE2_ARATH	8.6e-61	40.38	Probable carboxylesterase 2 OS=Arabidopsis thaliana GN=CXE2 PE=2 SV=1	[more]
CXE1_ARATH	1.5e-60	40.25	Probable carboxylesterase 1 OS=Arabidopsis thaliana GN=CXE1 PE=2 SV=1	[more]
CXE5_ARATH	5.6e-60	40.25	Probable carboxylesterase 5 OS=Arabidopsis thaliana GN=CXE5 PE=2 SV=1	[more]
CXE13_ARATH	1.8e-58	40.12	Probable carboxylesterase 13 OS=Arabidopsis thaliana GN=CXE13 PE=1 SV=1	[more]
CXE12_ARATH	6.8e-58	39.51	Probable carboxylesterase 12 OS=Arabidopsis thaliana GN=CXE12 PE=1 SV=1	[more]

BLAST of Lsi10G002950 vs. TrEMBL


Match Name	E-value	Identity	Description
Q0ZPW5_MALPM	2.0e-88	51.66	CXE carboxylesterase OS=Malus pumila GN=CXE5 PE=2 SV=1	[more]
M5VQ13_PRUPE	5.4e-86	51.84	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa009160mg PE=4 SV=1	[more]
A0A061E1M8_THECC	2.1e-82	49.51	Alpha/beta-Hydrolases superfamily protein, putative OS=Theobroma cacao GN=TCM_00...	[more]
W9SMM8_9ROSA	3.6e-82	51.66	Putative carboxylesterase 7 OS=Morus notabilis GN=L484_027556 PE=4 SV=1	[more]
F6HQD1_VITVI	6.8e-81	48.73	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_03s0063g00750 PE=4 SV=...	[more]

BLAST of Lsi10G002950 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G47480.1	4.9e-62	40.38	alpha/beta-Hydrolases superfamily protein	[more]
AT1G19190.1	8.3e-62	40.25	alpha/beta-Hydrolases superfamily protein	[more]
AT1G49660.1	3.1e-61	40.25	carboxyesterase 5	[more]
AT3G48700.1	1.0e-59	40.12	carboxyesterase 13	[more]
AT3G48690.1	3.8e-59	39.51	alpha/beta-Hydrolases superfamily protein	[more]

BLAST of Lsi10G002950 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|470117387\|ref\|XP_004294840.1\|	6.8e-90	54.18	PREDICTED: probable carboxylesterase 7 [Fragaria vesca subsp. vesca]	[more]
gi\|694353183\|ref\|XP_009358071.1\|	8.8e-90	52.98	PREDICTED: probable carboxylesterase 12 [Pyrus x bretschneideri]	[more]
gi\|694450813\|ref\|XP_009350720.1\|	2.2e-88	52.65	PREDICTED: probable carboxylesterase 12 [Pyrus x bretschneideri]	[more]
gi\|82697941\|gb\|ABB89005.1\|	2.8e-88	51.66	CXE carboxylesterase [Malus pumila]	[more]
gi\|657999750\|ref\|XP_008392308.1\|	8.3e-88	51.66	PREDICTED: probable carboxylesterase 7 [Malus domestica]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0016787	hydrolase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0008152	metabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR013094	AB_hydrolase_3

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008152	metabolic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0016787	hydrolase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi10G002950.1	Lsi10G002950.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013094	Alpha/beta hydrolase fold-3	PFAM	PF07859	Abhydrolase_3	coord: 75..281 score: 8.4
None	No IPR available	PANTHER	PTHR23024	MEMBER OF 'GDXG' FAMILY OF LIPOLYTIC ENZYMES	coord: 4..301 score: 7.8E
None	No IPR available	PANTHER	PTHR23024:SF131	CARBOXYLESTERASE 2-RELATED	coord: 4..301 score: 7.8E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi10G002950	Cla010255	Watermelon (97103) v1	lsiwmB043
Lsi10G002950	ClCG09G017360	Watermelon (Charleston Gray)	lsiwcgB034
Lsi10G002950	Bhi10G001755	Wax gourd	lsiwgoB074

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi10G002950	Cucumber (Gy14) v1	cgylsiB092
Lsi10G002950	Cucurbita maxima (Rimu)	cmalsiB507
Lsi10G002950	Cucurbita maxima (Rimu)	cmalsiB548
Lsi10G002950	Cucurbita moschata (Rifu)	cmolsiB491
Lsi10G002950	Cucurbita moschata (Rifu)	cmolsiB533
Lsi10G002950	Cucurbita pepo (Zucchini)	cpelsiB220
Lsi10G002950	Cucurbita pepo (Zucchini)	cpelsiB589
Lsi10G002950	Wild cucumber (PI 183967)	cpilsiB091
Lsi10G002950	Cucumber (Chinese Long) v2	culsiB093
Lsi10G002950	Melon (DHL92) v3.5.1	lsimeB057
Lsi10G002950	Cucumber (Gy14) v2	cgyblsiB086
Lsi10G002950	Melon (DHL92) v3.6.1	lsimedB059
Lsi10G002950	Silver-seed gourd	carlsiB731
Lsi10G002950	Silver-seed gourd	carlsiB961
Lsi10G002950	Cucumber (Chinese Long) v3	cuclsiB104
Lsi10G002950	Watermelon (97103) v2	lsiwmbB070