Lsi10G002740 (gene) Bottle gourd (USVL1VR-Ls)

NameLsi10G002740
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Locationchr10 : 4387234 .. 4390205 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGAAACTTTGGTTGCAAGATATTGGAGGTGATAAGTGAAGGGTTAGGACTTGGGAGAGGATACTTTGAGAGTGAAATAAATGAAAATCCATTGTTTCAAGTAAACCATTACCCACCATGCCCAAACCCTAGCTTGACTTTGGGATTGTTCCAACATTGTGATCCAAGCCTCATCACCATTTTGTTTCAAGATATCAATGGCCTTCAAGTCTTGAAGGATGGTCAATGGATTGATGTTGATCCTATTGAGGATACCCTTTTGATAAACTTTGGCTATCTTCTCGAGGTACGTTCATTTTGATTCTTACTACTTTTAATTTCCTTAAACTTAAGGCATTCGCCATCACGTTAAAAAATTACTTTGAATTTGTTAAGAATAAATTTCAATGTATTTACTATTTAAGAATGGTTGGAGATGACTTTTGGAAATAATGTTGTTAAAATAAACGGTTTAGGTTTGTTTTAATTTTAATATGAGAGACACTAAACTATATTACATTTTTAGAAAAATAAAATCAAAGCAACAAAAATTAAAAATGGCTATATGAGAAGTGTTTTTGTGAGAAATTGAGAAGTGTTTGGGACATTAGGTTAATTATTATAGTTAGAAGTTATTATAATCTGTAGGTTGTAATAATTTATATTTGAGGTACAAACTATTCTAGTTTGAGTTATAATTGTTTGTATTTGGATGCAGACTATTTTAGTTTGAGTAAGAAATAGTAAACACTGTAATAAAAAAGAGAATAAATAAGAAATAGTAAACATTGTAATAAAGAAAGATTTGAAATAATAATACTGTAATTAATTGTATGTTATATATAATAATTAGAAACACCAACTATATTATAATTCTAGCCATAAACATGAAACGGGATGCTATAATATCCCACTCCACCCGCTTGAAAGTAAAAAGTATGGATTAGCTTTTTCCAACCCATATTTGGTTGACAGTATTGTAGACCAAAATTTTTTACTACAAACTAGATGCAATTGTAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATAAAAAAAACCTATTGGTGTTAGATTTTTTTTCTTTTTCTTTTGACAATATGTGGGGTGAGGAAATCGAATCTTTGACTTCGAAGTTGATAGTACAAACACTATACTAGTTGAACTATGCTCACTTTAACTGTTATTAGTTGTTTTCTTCTATATGGAAACGTAATGAAATATAGATGATTACCATAATTCCCTATTTCTATTTCTAATTAATTAAAACGTGGTAACTAAATAATATATTATAAGAATTGAAACACCGTTTATCATTTTTATTTATTTATTCAAATTTTTATATTTTAAAAGTTAAACACTTAAATTAAAATTATACTAAATAATAATAATAGTAGAAGTAAAAGAAATTAACTTACTCATGAAATTAACTATAGAAGTATTTCTCTTTCCAAAAAAAGAAAAGAAAAGAAAGAAATTAACTATACAAGTAGTTCTTGGTTTTATATAGGTGATTAGCAATGGAAAGCTAAAAGCAACGGAACATCGAGTCGTGACAAATACGCAGACATCTCGACAAAGTTTAGTCTATCTAACTTTTCCTGAAAATGAAGTGATTATAGAACCAGCAAAAAGCCTGATAAATGAAGCTAATCCTCCACATTATCGCTCCATCAAGTACAAAGAATTTATCGGAGATTATGCTTCTATGTCTAAAGATAGAGAGACAATAATCAAGTATTTAGCAACAACCTAA

mRNA sequence

ATGAGAAACTTTGGTTGCAAGATATTGGAGGTGATAAGTGAAGGGTTAGGACTTGGGAGAGGATACTTTGAGAGTGAAATAAATGAAAATCCATTGTTTCAAGTAAACCATTACCCACCATGCCCAAACCCTAGCTTGACTTTGGGATTGTTCCAACATTGTGATCCAAGCCTCATCACCATTTTGTTTCAAGATATCAATGGCCTTCAAGTCTTGAAGGATGGTCAATGGATTGATGTTGATCCTATTGAGGATACCCTTTTGATAAACTTTGGCTATCTTCTCGAGGTGATTAGCAATGGAAAGCTAAAAGCAACGGAACATCGAGTCGTGACAAATACGCAGACATCTCGACAAAGTTTAGTCTATCTAACTTTTCCTGAAAATGAAGTGATTATAGAACCAGCAAAAAGCCTGATAAATGAAGCTAATCCTCCACATTATCGCTCCATCAAGTACAAAGAATTTATCGGAGATTATGCTTCTATGTCTAAAGATAGAGAGACAATAATCAAGTATTTAGCAACAACCTAA

Coding sequence (CDS)

ATGAGAAACTTTGGTTGCAAGATATTGGAGGTGATAAGTGAAGGGTTAGGACTTGGGAGAGGATACTTTGAGAGTGAAATAAATGAAAATCCATTGTTTCAAGTAAACCATTACCCACCATGCCCAAACCCTAGCTTGACTTTGGGATTGTTCCAACATTGTGATCCAAGCCTCATCACCATTTTGTTTCAAGATATCAATGGCCTTCAAGTCTTGAAGGATGGTCAATGGATTGATGTTGATCCTATTGAGGATACCCTTTTGATAAACTTTGGCTATCTTCTCGAGGTGATTAGCAATGGAAAGCTAAAAGCAACGGAACATCGAGTCGTGACAAATACGCAGACATCTCGACAAAGTTTAGTCTATCTAACTTTTCCTGAAAATGAAGTGATTATAGAACCAGCAAAAAGCCTGATAAATGAAGCTAATCCTCCACATTATCGCTCCATCAAGTACAAAGAATTTATCGGAGATTATGCTTCTATGTCTAAAGATAGAGAGACAATAATCAAGTATTTAGCAACAACCTAA

Protein sequence

MRNFGCKILEVISEGLGLGRGYFESEINENPLFQVNHYPPCPNPSLTLGLFQHCDPSLITILFQDINGLQVLKDGQWIDVDPIEDTLLINFGYLLEVISNGKLKATEHRVVTNTQTSRQSLVYLTFPENEVIIEPAKSLINEANPPHYRSIKYKEFIGDYASMSKDRETIIKYLATT
BLAST of Lsi10G002740 vs. Swiss-Prot
Match: HY6H_HYONI (Hyoscyamine 6-dioxygenase OS=Hyoscyamus niger GN=H6H PE=1 SV=1)

HSP 1 Score: 149.8 bits (377), Expect = 2.8e-35
Identity = 75/174 (43.10%), Postives = 107/174 (61.49%), Query Frame = 1

Query: 1   MRNFGCKILEVISEGLGLGRGYFESEINENPLFQVNHYPPCPNPSLTLGLFQHCDPSLIT 60
           +R    ++L+ I EGLGL  GYF++E+++  +   N+YPPCP+PS TLG   H D +LIT
Sbjct: 165 VRKLTMRMLDYICEGLGLKLGYFDNELSQIQMMLTNYYPPCPDPSSTLGSGGHYDGNLIT 224

Query: 61  ILFQDINGLQ--VLKDGQWIDVDPIEDTLLINFGYLLEVISNGKLKATEHRVVTNTQTSR 120
           +L QD+ GLQ  ++KD  WI V PI    ++N G  L+VI+N K + + HRVVT+    R
Sbjct: 225 LLQQDLPGLQQLIVKDATWIAVQPIPTAFVVNLGLTLKVITNEKFEGSIHRVVTDPTRDR 284

Query: 121 QSLVYLTFPENEVIIEPAKSLINEANPPHYRSIKYKEFIGDYASMSKDRETIIK 173
            S+  L  P+    IEPAK L+N+ NPP Y+   Y EF   Y S   D ++ +K
Sbjct: 285 VSIATLIGPDYSCTIEPAKELLNQDNPPLYKPYSYSEFADIYLSDKSDYDSGVK 338

BLAST of Lsi10G002740 vs. Swiss-Prot
Match: IDS3_HORVU (2'-deoxymugineic-acid 2'-dioxygenase OS=Hordeum vulgare GN=IDS3 PE=1 SV=3)

HSP 1 Score: 146.0 bits (367), Expect = 4.0e-34
Identity = 67/161 (41.61%), Postives = 102/161 (63.35%), Query Frame = 1

Query: 2   RNFGCKILEVISEGLGLGRGYFESEINE-NPLFQVNHYPPCPNPSLTLGLFQHCDPSLIT 61
           R+ G ++L ++ EG+G+   YFE +++  N +  +NHYP CPNP   LG   HCD +LIT
Sbjct: 157 RDVGKELLRLLCEGMGIQADYFEGDLSGGNVILNINHYPSCPNPDKALGQPPHCDRNLIT 216

Query: 62  ILFQD-INGLQVLKDGQWIDVDPIEDTLLINFGYLLEVISNGKLKATEHRVVTNTQTSRQ 121
           +L    +NGL+V   G WI VDP  +  ++NFG  LEV++NG LK+ EHR +TN+  +R 
Sbjct: 217 LLLPGAVNGLEVSYKGDWIKVDPAPNAFVVNFGQQLEVVTNGLLKSIEHRAMTNSALART 276

Query: 122 SLVYLTFPENEVIIEPAKSLINEANPPHYRSIKYKEFIGDY 161
           S+     P  E +I PAK  +++ NPP YR+  +++F+  Y
Sbjct: 277 SVATFIMPTQECLIGPAKEFLSKENPPCYRTTMFRDFMRIY 317

BLAST of Lsi10G002740 vs. Swiss-Prot
Match: IDS2_HORVU (Mugineic-acid 3-dioxygenase OS=Hordeum vulgare GN=IDS2 PE=2 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 7.0e-31
Identity = 66/157 (42.04%), Postives = 96/157 (61.15%), Query Frame = 1

Query: 2   RNFGCKILEVISEGLGLGRGYFESEINENPLF-QVNHYPPCPNPSLTLGLFQHCDPSLIT 61
           R    +IL ++ EGLGL   +F  +I+   +   +N+YPP PNPS TLGL  HCD  L+T
Sbjct: 156 RGVAMEILRLLCEGLGLRPDFFVGDISGGRVVVDINYYPPSPNPSRTLGLPPHCDRDLMT 215

Query: 62  ILFQD-INGLQVLKDGQWIDVDPIEDTLLINFGYLLEVISNGKLKATEHRVVTNTQTSRQ 121
           +L    + GL++   G WI V P+ ++L+INFG  LEV++NG LKA EHR  TN    R 
Sbjct: 216 VLLPGAVPGLEIAYKGGWIKVQPVPNSLVINFGLQLEVVTNGYLKAVEHRAATNFAEPRL 275

Query: 122 SLVYLTFPENEVIIEPAKSLINEANPPHYRSIKYKEF 157
           S+     P ++ ++ PA+  ++E NPP YR++   EF
Sbjct: 276 SVASFIVPADDCVVGPAEEFVSEDNPPRYRTLTVGEF 312

BLAST of Lsi10G002740 vs. Swiss-Prot
Match: DMR6_ARATH (Protein DOWNY MILDEW RESISTANCE 6 OS=Arabidopsis thaliana GN=DMR6 PE=1 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 7.8e-30
Identity = 72/174 (41.38%), Postives = 99/174 (56.90%), Query Frame = 1

Query: 1   MRNFGCKILEVISEGLGLGRGYFESEINENPLFQ-VNHYPPCPNPSLTLGLFQHCDPSLI 60
           +R  G KI E+ISE LGL + Y +  + E      VN+YPPCP P LT GL  H DP+ +
Sbjct: 159 VREVGFKIEELISESLGLEKDYMKKVLGEQGQHMAVNYYPPCPEPELTYGLPAHTDPNAL 218

Query: 61  TILFQD--INGLQVLKDGQWIDVDPIEDTLLINFGYLLEVISNGKLKATEHRVVTNTQTS 120
           TIL QD  + GLQ+L DGQW  V+P  D  +IN G  L+ +SNG  K+  HR VTNT+  
Sbjct: 219 TILLQDTTVCGLQILIDGQWFAVNPHPDAFVINIGDQLQALSNGVYKSVWHRAVTNTENP 278

Query: 121 RQSLVYLTFPENEVIIEPAKSL---INEANPPHYRSIKYKEFIGDYASMSKDRE 169
           R S+     P +  ++ PAK L    ++   P Y+   Y E+   + S + D+E
Sbjct: 279 RLSVASFLCPADCAVMSPAKPLWEAEDDETKPVYKDFTYAEYYKKFWSRNLDQE 332

BLAST of Lsi10G002740 vs. Swiss-Prot
Match: DLO2_ARATH (Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana GN=DLO2 PE=2 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 2.3e-29
Identity = 69/168 (41.07%), Postives = 100/168 (59.52%), Query Frame = 1

Query: 8   ILEVISEGLGLGRGYFESEINENPLFQ-VNHYPPCPNPSLTLGLFQHCDPSLITILFQD- 67
           +LE ISE LGL +    + I ++     +N+YP CP P LT GL  H D +LIT+L QD 
Sbjct: 173 LLEAISESLGLAKDRVSNTIGKHGQHMAINYYPRCPQPELTYGLPGHKDANLITVLLQDE 232

Query: 68  INGLQVLKDGQWIDVDPIEDTLLINFGYLLEVISNGKLKATEHRVVTNTQTSRQSLVYLT 127
           ++GLQV KDG+WI V+P+ +T ++N G  ++VISN K K+  HR V N+   R S+    
Sbjct: 233 VSGLQVFKDGKWIAVNPVPNTFIVNLGDQMQVISNEKYKSVLHRAVVNSDMERISIPTFY 292

Query: 128 FPENEVIIEPAKSLINEA--NPPHYRSIKYKEFIGDYASMSKDRETII 172
            P  + +I PA+ LINE   +P  YR+  Y E+   +   + D E+ I
Sbjct: 293 CPSEDAVISPAQELINEEEDSPAIYRNFTYAEYFEKFWDTAFDTESCI 340

BLAST of Lsi10G002740 vs. TrEMBL
Match: A0A0A0K5H0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G375850 PE=3 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 9.8e-64
Identity = 112/175 (64.00%), Postives = 142/175 (81.14%), Query Frame = 1

Query: 1   MRNFGCKILEVISEGLGLGRGYFESEINENPLFQVNHYPPCPNPSLTLGLFQHCDPSLIT 60
           +  F  +ILEV+SEGLGL +GYF  E+++NP F  +HYPPCPNPSLT+GL QH DP+LIT
Sbjct: 159 IEKFASEILEVLSEGLGLEKGYFRGEMSKNPEFLAHHYPPCPNPSLTVGLSQHADPTLIT 218

Query: 61  ILFQDINGLQVLKDGQWIDVDPIEDTLLINFGYLLEVISNGKLKATEHRVVTNTQTSRQS 120
           ILFQD+NGLQ LKDGQWIDVDP+ D  L+N GY+ EVISNGKLKA EHRVVTN+ TSRQ+
Sbjct: 219 ILFQDVNGLQFLKDGQWIDVDPLPDAFLVNLGYVFEVISNGKLKAAEHRVVTNSTTSRQT 278

Query: 121 LVYLTFPENEVIIEPAKSLINEANPPHYRSIKYKEFIGDYASMSKDRETIIKYLA 176
           LVY+ +PEN+V IEP+K  INEANPPHYRS+++K+F     S   DR+T +++++
Sbjct: 279 LVYVVYPENDVTIEPSKCFINEANPPHYRSLEFKDFYNKLMSKPPDRKTAMEFIS 333

BLAST of Lsi10G002740 vs. TrEMBL
Match: A0A0A0K5H3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G378370 PE=3 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 2.8e-63
Identity = 110/176 (62.50%), Postives = 145/176 (82.39%), Query Frame = 1

Query: 1   MRNFGCKILEVISEGLGLGRGYFESEINENPLFQVNHYPPCPNPSLTLGLFQHCDPSLIT 60
           MR    +ILE++SEGLGLG+GYF  E++ENPL  VNHYPPCPNPSLTLGL QHCDPSLIT
Sbjct: 163 MRKLVLEILELMSEGLGLGKGYFGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLIT 222

Query: 61  ILFQDINGLQVLKDGQWIDVDPIEDTLLINFGYLLEVISNGKLKATEHRVVTNTQTSRQS 120
           ILFQD+NGLQVLKDGQWI V PI++  ++N G++L++I+NGKL+A EHR VTN++TSRQS
Sbjct: 223 ILFQDVNGLQVLKDGQWIGVQPIDNAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQS 282

Query: 121 LVYLTFPENEVIIEPAKSLINEANPPHYRSIKYKEFIGDYASMSKDRETIIKYLAT 177
           L YL +P++E ++EPAK +INEANPP YRS+ +K+F  +Y   + D + +++Y+ T
Sbjct: 283 LTYLVYPKDEAMMEPAKCMINEANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIET 338

BLAST of Lsi10G002740 vs. TrEMBL
Match: A0A0A0K745_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G375860 PE=3 SV=1)

HSP 1 Score: 243.4 bits (620), Expect = 2.0e-61
Identity = 109/175 (62.29%), Postives = 138/175 (78.86%), Query Frame = 1

Query: 1   MRNFGCKILEVISEGLGLGRGYFESEINENPLFQVNHYPPCPNPSLTLGLFQHCDPSLIT 60
           M+ F  +ILEV+SEGLGL + YF   ++ NP F V+HYPPCPNPSLTLGL +H DP++IT
Sbjct: 159 MKKFSLEILEVLSEGLGLEKEYFSGGMSNNPEFLVHHYPPCPNPSLTLGLNKHADPTVIT 218

Query: 61  ILFQDINGLQVLKDGQWIDVDPIEDTLLINFGYLLEVISNGKLKATEHRVVTNTQTSRQS 120
           ILFQD+NGLQ LKDG+WIDVDP+ D  L+N GY+ EVISNGK+KA EHRVVTNT TSRQS
Sbjct: 219 ILFQDVNGLQFLKDGEWIDVDPLPDAFLVNLGYVFEVISNGKMKAAEHRVVTNTTTSRQS 278

Query: 121 LVYLTFPENEVIIEPAKSLINEANPPHYRSIKYKEFIGDYASMSKDRETIIKYLA 176
           L Y  +PEN + IEP+K  INEANPPHYR++++K+F+    S   DRE  +K+++
Sbjct: 279 LAYGVYPENNMTIEPSKCFINEANPPHYRTLEFKDFVNKLRSKHPDREMAMKFIS 333

BLAST of Lsi10G002740 vs. TrEMBL
Match: A0A0A0K4U9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G376360 PE=3 SV=1)

HSP 1 Score: 236.5 bits (602), Expect = 2.5e-59
Identity = 108/174 (62.07%), Postives = 135/174 (77.59%), Query Frame = 1

Query: 1   MRNFGCKILEVISEGLGLGRGYFESEINENPLFQVNHYPPCPNPSLTLGLFQHCDPSLIT 60
           M  F  +I+++ISEGLGLG+GYFE E++       NHYP CPNPSLT+GL QHCD  L+T
Sbjct: 158 MEKFSREIIDLISEGLGLGQGYFEGEMSSYRRLTANHYPICPNPSLTMGLNQHCDRDLMT 217

Query: 61  ILFQDINGLQVLKDGQWIDVDPIEDTLLINFGYLLEVISNGKLKATEHRVVTNTQTSRQS 120
           ILFQD++GLQV KDG WI +DPI+D  ++NFGYLLEVISNGKLKA EHRVVTN + SRQS
Sbjct: 218 ILFQDVSGLQVFKDGHWISIDPIDDAFVVNFGYLLEVISNGKLKAVEHRVVTNAEASRQS 277

Query: 121 LVYLTFPENEVIIEPAKSLINEANPPHYRSIKYKEFIGDYASMSKDRETIIKYL 175
             Y+ FPE E+IIEPAK LINEANPPHYRS K ++F   + ++S + E  +K++
Sbjct: 278 FGYVIFPEKEMIIEPAKCLINEANPPHYRSFKVEDFNRHFFAVSNNNEETMKFI 331

BLAST of Lsi10G002740 vs. TrEMBL
Match: A0A067FM54_CITSI (Uncharacterized protein (Fragment) OS=Citrus sinensis GN=CISIN_1g048024mg PE=3 SV=1)

HSP 1 Score: 201.8 bits (512), Expect = 6.8e-49
Identity = 87/177 (49.15%), Postives = 132/177 (74.58%), Query Frame = 1

Query: 1   MRNFGCKILEVISEGLGLGRGYFESEINENPLFQVNHYPPCPNPSLTLGLFQHCDPSLIT 60
           +R    KILE++SEGL L  GYF S+++E+P+  +NHYPPCP+PSLTLGL +H DP +++
Sbjct: 164 VRKLSSKILELLSEGLKLSSGYFSSDLSESPILLINHYPPCPDPSLTLGLARHRDPGVVS 223

Query: 61  ILFQ-DINGLQVLKDGQWIDVDPIEDTLLINFGYLLEVISNGKLKATEHRVVTNTQTSRQ 120
           I+ Q D++GLQV KD +WI V+PI    ++N GY+L++ISNGKLK  EHRVVTN+  +R 
Sbjct: 224 IVLQGDVHGLQVFKDEEWIGVEPIPHAFVVNIGYVLQIISNGKLKGAEHRVVTNSTDART 283

Query: 121 SLVYLTFPENEVIIEPAKSLINEANPPHYRSIKYKEFIGDYASMSKDRETIIKYLAT 177
           ++ +  +P N+ +IEP K+L+N  +PP YR++K+K+F  D+ S S D E + K++++
Sbjct: 284 TVSFFVYPSNDSLIEPEKALVNACDPPIYRALKFKDFHVDFLSKSADAEAVQKFISS 340

BLAST of Lsi10G002740 vs. TAIR10
Match: AT3G19000.1 (AT3G19000.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 132.5 bits (332), Expect = 2.6e-31
Identity = 69/154 (44.81%), Postives = 94/154 (61.04%), Query Frame = 1

Query: 7   KILEVISEGLGLGRGYFESEINENPLF-QVNHYPPCPNPSLTLGLFQHCDPSLITILFQD 66
           ++LE++S  LGL         NE   F + NHYPPCPNP L LG+ +H D   +T+L QD
Sbjct: 175 RLLELVSISLGLPGDRLTGFFNEQTSFLRFNHYPPCPNPELALGVGRHKDGGALTVLAQD 234

Query: 67  -INGLQVLK--DGQWIDVDPIEDTLLINFGYLLEVISNGKLKATEHRVVTNTQTSRQSLV 126
            + GLQV +  DGQWI V PI D L+IN G  ++V +N +  + EHRVV NT   R S+ 
Sbjct: 235 SVGGLQVSRRSDGQWIPVKPISDALIINMGNCIQVWTNDEYWSAEHRVVVNTSKERFSIP 294

Query: 127 YLTFPENEVIIEPAKSLINEANPPHYRSIKYKEF 157
           +  FP +E  IEP + LI+E NPP Y+   + +F
Sbjct: 295 FFFFPSHEANIEPLEELISEENPPCYKKYNWGKF 328

BLAST of Lsi10G002740 vs. TAIR10
Match: AT5G24530.1 (AT5G24530.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 131.7 bits (330), Expect = 4.4e-31
Identity = 72/174 (41.38%), Postives = 99/174 (56.90%), Query Frame = 1

Query: 1   MRNFGCKILEVISEGLGLGRGYFESEINENPLFQ-VNHYPPCPNPSLTLGLFQHCDPSLI 60
           +R  G KI E+ISE LGL + Y +  + E      VN+YPPCP P LT GL  H DP+ +
Sbjct: 159 VREVGFKIEELISESLGLEKDYMKKVLGEQGQHMAVNYYPPCPEPELTYGLPAHTDPNAL 218

Query: 61  TILFQD--INGLQVLKDGQWIDVDPIEDTLLINFGYLLEVISNGKLKATEHRVVTNTQTS 120
           TIL QD  + GLQ+L DGQW  V+P  D  +IN G  L+ +SNG  K+  HR VTNT+  
Sbjct: 219 TILLQDTTVCGLQILIDGQWFAVNPHPDAFVINIGDQLQALSNGVYKSVWHRAVTNTENP 278

Query: 121 RQSLVYLTFPENEVIIEPAKSL---INEANPPHYRSIKYKEFIGDYASMSKDRE 169
           R S+     P +  ++ PAK L    ++   P Y+   Y E+   + S + D+E
Sbjct: 279 RLSVASFLCPADCAVMSPAKPLWEAEDDETKPVYKDFTYAEYYKKFWSRNLDQE 332

BLAST of Lsi10G002740 vs. TAIR10
Match: AT4G10490.1 (AT4G10490.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 130.2 bits (326), Expect = 1.3e-30
Identity = 69/168 (41.07%), Postives = 100/168 (59.52%), Query Frame = 1

Query: 8   ILEVISEGLGLGRGYFESEINENPLFQ-VNHYPPCPNPSLTLGLFQHCDPSLITILFQD- 67
           +LE ISE LGL +    + I ++     +N+YP CP P LT GL  H D +LIT+L QD 
Sbjct: 173 LLEAISESLGLAKDRVSNTIGKHGQHMAINYYPRCPQPELTYGLPGHKDANLITVLLQDE 232

Query: 68  INGLQVLKDGQWIDVDPIEDTLLINFGYLLEVISNGKLKATEHRVVTNTQTSRQSLVYLT 127
           ++GLQV KDG+WI V+P+ +T ++N G  ++VISN K K+  HR V N+   R S+    
Sbjct: 233 VSGLQVFKDGKWIAVNPVPNTFIVNLGDQMQVISNEKYKSVLHRAVVNSDMERISIPTFY 292

Query: 128 FPENEVIIEPAKSLINEA--NPPHYRSIKYKEFIGDYASMSKDRETII 172
            P  + +I PA+ LINE   +P  YR+  Y E+   +   + D E+ I
Sbjct: 293 CPSEDAVISPAQELINEEEDSPAIYRNFTYAEYFEKFWDTAFDTESCI 340

BLAST of Lsi10G002740 vs. TAIR10
Match: AT5G59540.1 (AT5G59540.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 126.3 bits (316), Expect = 1.8e-29
Identity = 67/159 (42.14%), Postives = 91/159 (57.23%), Query Frame = 1

Query: 3   NFGCKILEVISEGLGLGRGYFESEINENPLFQVNHY-PPCPNPSLTLGLFQHCDPSLITI 62
           +FG  + E++SE LGL     ES      L  + HY PPCP P LTLG+ +H D S +T+
Sbjct: 188 SFGGLLFELLSEALGLKSQTLESMDCVKTLLMICHYYPPCPQPDLTLGITKHSDNSFLTL 247

Query: 63  LFQD-INGLQVLKDGQWIDVDPIEDTLLINFGYLLEVISNGKLKATEHRVVTNTQTSRQS 122
           L QD I GLQ+L    W+DV PI   L++N G  L++I+N K  + EHRV+ N Q  R S
Sbjct: 248 LLQDNIGGLQILHQDSWVDVSPIHGALVVNIGDFLQLITNDKFVSVEHRVLANRQGPRIS 307

Query: 123 LVYL---TFPENEVIIEPAKSLINEANPPHYRSIKYKEF 157
           +      +   N  +  P K L++E NPP YR I  KE+
Sbjct: 308 VASFFSSSMRPNSRVYGPMKELVSEENPPKYRDITIKEY 346

BLAST of Lsi10G002740 vs. TAIR10
Match: AT1G06620.1 (AT1G06620.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 125.6 bits (314), Expect = 3.1e-29
Identity = 72/177 (40.68%), Postives = 98/177 (55.37%), Query Frame = 1

Query: 5   GCKILEVISEGLGLGRGYFESEINENPLFQVNHY-PPCPNPSLTLGLFQHCDPSLITILF 64
           G  + E++SE LGL   + +     N L  + HY PPCP P LTLGL +H D S +TIL 
Sbjct: 189 GKLLFELLSEALGLNTNHLKDMDCTNSLLLLGHYYPPCPQPDLTLGLTKHSDNSFLTILL 248

Query: 65  QD-INGLQVLKDGQWIDVDPIEDTLLINFGYLLEVISNGKLKATEHRVVTNTQTSRQSLV 124
           QD I GLQVL D  W+DV P+   L++N G LL++I+N K  + EHRV+ N    R S+ 
Sbjct: 249 QDHIGGLQVLHDQYWVDVPPVPGALVVNVGDLLQLITNDKFISVEHRVLANVAGPRISVA 308

Query: 125 -----YLTFPENEVIIEPAKSLINEANPPHYRSIKYKEFIGDYASMSKDRETIIKYL 175
                YL    N  +  P K +++E NPP+YR     E+   Y S   D  + + YL
Sbjct: 309 CFFSSYLM--ANPRVYGPIKEILSEENPPNYRDTTITEYAKFYRSKGFDGTSGLLYL 363

BLAST of Lsi10G002740 vs. NCBI nr
Match: gi|449462457|ref|XP_004148957.1| (PREDICTED: hyoscyamine 6-dioxygenase-like [Cucumis sativus])

HSP 1 Score: 251.1 bits (640), Expect = 1.4e-63
Identity = 112/175 (64.00%), Postives = 142/175 (81.14%), Query Frame = 1

Query: 1   MRNFGCKILEVISEGLGLGRGYFESEINENPLFQVNHYPPCPNPSLTLGLFQHCDPSLIT 60
           +  F  +ILEV+SEGLGL +GYF  E+++NP F  +HYPPCPNPSLT+GL QH DP+LIT
Sbjct: 159 IEKFASEILEVLSEGLGLEKGYFRGEMSKNPEFLAHHYPPCPNPSLTVGLSQHADPTLIT 218

Query: 61  ILFQDINGLQVLKDGQWIDVDPIEDTLLINFGYLLEVISNGKLKATEHRVVTNTQTSRQS 120
           ILFQD+NGLQ LKDGQWIDVDP+ D  L+N GY+ EVISNGKLKA EHRVVTN+ TSRQ+
Sbjct: 219 ILFQDVNGLQFLKDGQWIDVDPLPDAFLVNLGYVFEVISNGKLKAAEHRVVTNSTTSRQT 278

Query: 121 LVYLTFPENEVIIEPAKSLINEANPPHYRSIKYKEFIGDYASMSKDRETIIKYLA 176
           LVY+ +PEN+V IEP+K  INEANPPHYRS+++K+F     S   DR+T +++++
Sbjct: 279 LVYVVYPENDVTIEPSKCFINEANPPHYRSLEFKDFYNKLMSKPPDRKTAMEFIS 333

BLAST of Lsi10G002740 vs. NCBI nr
Match: gi|449458500|ref|XP_004146985.1| (PREDICTED: hyoscyamine 6-dioxygenase-like [Cucumis sativus])

HSP 1 Score: 249.6 bits (636), Expect = 4.1e-63
Identity = 110/176 (62.50%), Postives = 145/176 (82.39%), Query Frame = 1

Query: 1   MRNFGCKILEVISEGLGLGRGYFESEINENPLFQVNHYPPCPNPSLTLGLFQHCDPSLIT 60
           MR    +ILE++SEGLGLG+GYF  E++ENPL  VNHYPPCPNPSLTLGL QHCDPSLIT
Sbjct: 163 MRKLVLEILELMSEGLGLGKGYFGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLIT 222

Query: 61  ILFQDINGLQVLKDGQWIDVDPIEDTLLINFGYLLEVISNGKLKATEHRVVTNTQTSRQS 120
           ILFQD+NGLQVLKDGQWI V PI++  ++N G++L++I+NGKL+A EHR VTN++TSRQS
Sbjct: 223 ILFQDVNGLQVLKDGQWIGVQPIDNAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQS 282

Query: 121 LVYLTFPENEVIIEPAKSLINEANPPHYRSIKYKEFIGDYASMSKDRETIIKYLAT 177
           L YL +P++E ++EPAK +INEANPP YRS+ +K+F  +Y   + D + +++Y+ T
Sbjct: 283 LTYLVYPKDEAMMEPAKCMINEANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIET 338

BLAST of Lsi10G002740 vs. NCBI nr
Match: gi|659100769|ref|XP_008451257.1| (PREDICTED: hyoscyamine 6-dioxygenase-like [Cucumis melo])

HSP 1 Score: 246.1 bits (627), Expect = 4.5e-62
Identity = 112/175 (64.00%), Postives = 140/175 (80.00%), Query Frame = 1

Query: 1   MRNFGCKILEVISEGLGLGRGYFESEINENPLFQVNHYPPCPNPSLTLGLFQHCDPSLIT 60
           +  F  +ILEV+SEGLGL +GY   E+++NP   V+HYPPCPNPSLT+GL QH DP+LIT
Sbjct: 161 IEKFALEILEVLSEGLGLEKGYLRGEMSKNPELFVHHYPPCPNPSLTIGLNQHADPTLIT 220

Query: 61  ILFQDINGLQVLKDGQWIDVDPIEDTLLINFGYLLEVISNGKLKATEHRVVTNTQTSRQS 120
           ILFQD+NGLQ LKDGQWIDVDP+ +  L+N GY+LEVISNGK+KA EHRVVTNT TSRQS
Sbjct: 221 ILFQDVNGLQFLKDGQWIDVDPLPNAFLVNLGYVLEVISNGKMKAVEHRVVTNTTTSRQS 280

Query: 121 LVYLTFPENEVIIEPAKSLINEANPPHYRSIKYKEFIGDYASMSKDRETIIKYLA 176
           L Y+ +P N+V IEP+K  INEANPPHY S+++K+FI    S   DRET +K+++
Sbjct: 281 LAYVVYPGNDVNIEPSKCFINEANPPHYLSLEFKDFIKKLMSKPPDRETALKFIS 335

BLAST of Lsi10G002740 vs. NCBI nr
Match: gi|659100775|ref|XP_008451261.1| (PREDICTED: hyoscyamine 6-dioxygenase-like [Cucumis melo])

HSP 1 Score: 245.7 bits (626), Expect = 5.9e-62
Identity = 109/176 (61.93%), Postives = 142/176 (80.68%), Query Frame = 1

Query: 1   MRNFGCKILEVISEGLGLGRGYFESEINENPLFQVNHYPPCPNPSLTLGLFQHCDPSLIT 60
           MR    +ILE++SEGLGLG+GY   E++ENPL  VNHYPPCPNPSLTLGL QHCDPSLIT
Sbjct: 163 MRKLVLEILELMSEGLGLGKGYLGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLIT 222

Query: 61  ILFQDINGLQVLKDGQWIDVDPIEDTLLINFGYLLEVISNGKLKATEHRVVTNTQTSRQS 120
           ILFQD+NGLQVLKDGQWI V PI+   ++N G++L++I+NGKL+A EHR VTN++TSRQS
Sbjct: 223 ILFQDVNGLQVLKDGQWIGVQPIDKAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQS 282

Query: 121 LVYLTFPENEVIIEPAKSLINEANPPHYRSIKYKEFIGDYASMSKDRETIIKYLAT 177
           L YL +P++E  +EPAK +INEANPP YRS+ +K+F  +Y   + D + +++Y+ T
Sbjct: 283 LTYLVYPKDEATMEPAKCMINEANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIET 338

BLAST of Lsi10G002740 vs. NCBI nr
Match: gi|778730376|ref|XP_011659768.1| (PREDICTED: hyoscyamine 6-dioxygenase-like [Cucumis sativus])

HSP 1 Score: 243.4 bits (620), Expect = 2.9e-61
Identity = 109/175 (62.29%), Postives = 138/175 (78.86%), Query Frame = 1

Query: 1   MRNFGCKILEVISEGLGLGRGYFESEINENPLFQVNHYPPCPNPSLTLGLFQHCDPSLIT 60
           M+ F  +ILEV+SEGLGL + YF   ++ NP F V+HYPPCPNPSLTLGL +H DP++IT
Sbjct: 8   MKKFSLEILEVLSEGLGLEKEYFSGGMSNNPEFLVHHYPPCPNPSLTLGLNKHADPTVIT 67

Query: 61  ILFQDINGLQVLKDGQWIDVDPIEDTLLINFGYLLEVISNGKLKATEHRVVTNTQTSRQS 120
           ILFQD+NGLQ LKDG+WIDVDP+ D  L+N GY+ EVISNGK+KA EHRVVTNT TSRQS
Sbjct: 68  ILFQDVNGLQFLKDGEWIDVDPLPDAFLVNLGYVFEVISNGKMKAAEHRVVTNTTTSRQS 127

Query: 121 LVYLTFPENEVIIEPAKSLINEANPPHYRSIKYKEFIGDYASMSKDRETIIKYLA 176
           L Y  +PEN + IEP+K  INEANPPHYR++++K+F+    S   DRE  +K+++
Sbjct: 128 LAYGVYPENNMTIEPSKCFINEANPPHYRTLEFKDFVNKLRSKHPDREMAMKFIS 182

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
HY6H_HYONI2.8e-3543.10Hyoscyamine 6-dioxygenase OS=Hyoscyamus niger GN=H6H PE=1 SV=1[more]
IDS3_HORVU4.0e-3441.612'-deoxymugineic-acid 2'-dioxygenase OS=Hordeum vulgare GN=IDS3 PE=1 SV=3[more]
IDS2_HORVU7.0e-3142.04Mugineic-acid 3-dioxygenase OS=Hordeum vulgare GN=IDS2 PE=2 SV=1[more]
DMR6_ARATH7.8e-3041.38Protein DOWNY MILDEW RESISTANCE 6 OS=Arabidopsis thaliana GN=DMR6 PE=1 SV=1[more]
DLO2_ARATH2.3e-2941.07Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana GN=DLO2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0K5H0_CUCSA9.8e-6464.00Uncharacterized protein OS=Cucumis sativus GN=Csa_7G375850 PE=3 SV=1[more]
A0A0A0K5H3_CUCSA2.8e-6362.50Uncharacterized protein OS=Cucumis sativus GN=Csa_7G378370 PE=3 SV=1[more]
A0A0A0K745_CUCSA2.0e-6162.29Uncharacterized protein OS=Cucumis sativus GN=Csa_7G375860 PE=3 SV=1[more]
A0A0A0K4U9_CUCSA2.5e-5962.07Uncharacterized protein OS=Cucumis sativus GN=Csa_7G376360 PE=3 SV=1[more]
A0A067FM54_CITSI6.8e-4949.15Uncharacterized protein (Fragment) OS=Citrus sinensis GN=CISIN_1g048024mg PE=3 S... [more]
Match NameE-valueIdentityDescription
AT3G19000.12.6e-3144.81 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT5G24530.14.4e-3141.38 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT4G10490.11.3e-3041.07 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT5G59540.11.8e-2942.14 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT1G06620.13.1e-2940.68 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
Match NameE-valueIdentityDescription
gi|449462457|ref|XP_004148957.1|1.4e-6364.00PREDICTED: hyoscyamine 6-dioxygenase-like [Cucumis sativus][more]
gi|449458500|ref|XP_004146985.1|4.1e-6362.50PREDICTED: hyoscyamine 6-dioxygenase-like [Cucumis sativus][more]
gi|659100769|ref|XP_008451257.1|4.5e-6264.00PREDICTED: hyoscyamine 6-dioxygenase-like [Cucumis melo][more]
gi|659100775|ref|XP_008451261.1|5.9e-6261.93PREDICTED: hyoscyamine 6-dioxygenase-like [Cucumis melo][more]
gi|778730376|ref|XP_011659768.1|2.9e-6162.29PREDICTED: hyoscyamine 6-dioxygenase-like [Cucumis sativus][more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
Vocabulary: Molecular Function
TermDefinition
GO:0016491oxidoreductase activity
Vocabulary: INTERPRO
TermDefinition
IPR027443IPNS-like
IPR005123Oxoglu/Fe-dep_dioxygenase
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0005575 cellular_component
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016491 oxidoreductase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi10G002740.1Lsi10G002740.1mRNA


Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005123Oxoglutarate/iron-dependent dioxygenasePFAMPF031712OG-FeII_Oxycoord: 33..127
score: 1.8
IPR005123Oxoglutarate/iron-dependent dioxygenasePROFILEPS51471FE2OG_OXYcoord: 28..127
score: 1
IPR027443Isopenicillin N synthase-likeGENE3DG3DSA:2.60.120.330coord: 1..168
score: 1.4
NoneNo IPR availablePANTHERPTHR10209OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEINcoord: 1..170
score: 1.7
NoneNo IPR availablePANTHERPTHR10209:SF124SUBFAMILY NOT NAMEDcoord: 1..170
score: 1.7
NoneNo IPR availableunknownSSF51197Clavaminate synthase-likecoord: 1..160
score: 6.73

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None