Lsi10G002240 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi10G002240
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Gamma-tocopherol methyltransferase
Location	chr10 : 3636395 .. 3639529 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTAGACTCAATTACCCTTTCTAAAGAAAAAAGAAAAAAAAAAGAAAAAAGAAATAACGCCTGTTCTTTCTGTATCTTCTCTCTCAGCATCTCTGTGTTGCTTTACGTTCCCAACAACTTGGCATCGGCCATTGCTCCCTTATTTATATTATTACCAACAATGAGCTTCACCGCTGCAACTCCAATCCATACGTCGTCGTATCCCCCTTCTTCTCTCCATTCCAATCGCTTCAACCCCACAATCCTCTCTCCCATCCCTCTCCGCCGTTCCTTTTCTTCTTTCATCCCCCGCCGCACCCCTCCTCTGCTTTCCGCTTCAGCCACGACGCCCGCAGACATCCAGATGGACGCCGATCGCCTTCAGAAGGGAATCGCCGAGTTCTACGATGAATCATCGGGGCTTTGGGAGAGCGTTTGGGGCGAACATATGCACCATGGATTTTACGATCCTGATTCTTCCATTTCCCTCTCCGATCACCGCTCTGCGCAAATCCGGATGATCGAGGAGACGCTCCGGTTCGCCGGCGTGGAGGCGGCGACGGCAGCGGTGGACGTTGGGTGTGGGATTGGGGGAAGTTCTAGGTATTTGGCGAAGAAGTTTGGGGCGAAATGTAGAGGTATAACTTTGAGCCCTGTCCAAGCTAAACGCGCTCAAGCGATTGCTGCCGCTGAAGGATTAAGTGATAAGGTGTGTACGGTCTTTGAAACCTCTGTTCTTATTTGTTCTTACTCCGTTTATCATTTTTGTTTTTCTTTGGAAATGCTCCGTTACGCTTTTGTTTTGACGTTTTCTTTGCTTTGATACTAAACTTGAAAATTAGTCCTCAATCAACTAGTACAACCAAAATAATTTGTACTTTCTAGATTAGTCCTTTTCAAGTTTGGTTGCAGAAATGGTTAAAAAAATAATGCTTCTTTTTAACCATTCTAAGTTTAGTACTTGAAATTAAATGATTAGTGGTATGTGTAATCTTTGAAATGAATACCAGAGAAATGTATGTTAATTTGAACTTCTAACTTTTTGGTTAGAAGAATATATAATGACTTAACCAATAAAGCTATATTGATATTAAGTGACAAAATTGTAGAAGAAGTTGAAAGAGTTACTTTATTTTCTTGGCGTTCAACAATATGTGAAGACGGGAATATATACTTTATTCAGAGATATAATATGGAATAGGAAGGTATCTAAAATGGTTAAAATGAAAGTTTTAACATAAATATGGGATTGACATGATCATAAGGAGGATTTTTGGTGGGTGGTAGGTGTGTTTTGAAGTGGCAGATGCTCTGAACCAACCATTCTCAGATGGGGAATTTGATCTGGTATGGTCCATGGAGAGTGGGGAGCACATGCCTGACAAATCCAAGGTTATTTTTTCTTTATACTCTCTCACTTTAATGATTTCCATTTCTCTTAAAGTCAGTGTTTGTTTGTGTTGTCTTATCTGTGTTTTGTTGATATGCTTCTTTAAAAACTCTGCAGTTTGTGAGTGAACTAGTTAGAGTTGCAGCCCCAGGAAGTACCATAATTATAGTTACATGGTGCCATAGAAACCTTGGGCCTTCTGAAAACTCCTTGCAGCCATGGGAGGAGAAGCTCCTTCAGAAGATTTGTGATGGCTTCTATCTCCCTGCTTGGTGTTCTACTGCTGATTATGTCAAATTACTCGAATCCCACAATCTACAGGTACTATTGTCTTTATACTTTGAGCATCGTTTCTATTTATTGATGGTCCGAGTTGACATAGTGCCAATAACAAGATTCAAGAGAATGAATGGTTCAAATCATTTAGATAAGGAGGGGAGATGTGATATTTGAAGAATTGTAAAGACATAAAATTGATGTTTTTAGTCGTGGATCCATGAACTTCTTGAGCCAAACAAAAAATGGCATAATTCCACACTACTCTACTCCTACTTCTCCAACTCCTTGGCTTAGGGTGTTTGGCCAAGGAGTTCGAGGAGTAAGAGTTGAGTGGAGTGAATTTTGAATTCCACTCCTTGTTTAGCCCAAGGAGTTCGTGGGTCCCACGACTAAAAGAACATTAATTTTATACCTTATTAACTTTTTACACTGTTGGTCTCAAGAGTTCATAACTCTCTAGACTTATAACTCCATGAAATACACAACTCCACTCTTTGTCCTGAACGCCCCAACCTTTAGGGAGCGTTTGGGACAAGGAGTGAAGTTGTTTAGTATGAAGAGTTGTTTAGTTGTGAGGCCACAGTGTAAGAAGTTAATAAAGTCATGGAAGTGATAATTTTTAGTCGTTGAAAAAATTCTTCGAGCAAATAAGTAGTGAAGTTTCACTCTCCCAACTCTTACTTTTTGAAATAAATGTGCACCTTAATGATTGTATCTTATAAGTAAATATTGTAAAGTTAAATAGTTATGTCGTGAGATTGGTCGAGAGGTATGGATCAATATAATTAAGTTGGTGCCAACATTCATCTCACATTAATCTTTGAATCTGAATCATAATTTGGGGACTGATTAAGAGAGACATTATTAGAAATTTTCAGGATCATTCACCATCATAATTTAATGAATTAATGGAACCCACGTGGGAATGAGAGTTAATTTTGGGATGTAGCGGAACATCTTGATAAAGCTGAATATTTATTTATTTATTTATTTGAATAAACTAAAAGGTTTTTTTCTTTATTATTTTTACTATTGGAAAATGATATGTAAAAAATGGTGTTTGGTGTTGATAGGATATAAAGACAGCAGACTGGTCTCAGAATGTTGCTCCGTTTTGGCCTGCAGTTATTCGGTCTGCTTTGACTTGGAAAGGCTTCACATCACTCTTACGAAGTGGTAAATATTCCTGACACTAAAAAATAATAATAAAAAAAAATCAATTTTACTCAACTGAAAATAAAATAAAATAATCAATTTTTTGTTAAAAACTTTTCCAAAATTCCTTTCTCAAACCACACTTTACTTGTTATTGCTTTTAATGGTCTAAATTTAAATTATGTGATTTTATTAAATCATATTGAAATTGATTAAATAATAATAATAGAAGAAAAAAAAGTATGTAATATATTTTTAAAATTTATAAAAAGAAAGTTAATAGGGACTGATTTTTTTTTCTTATAAAAAAACTAAAAATAAATTAGGAGTAG

mRNA sequence

ATTAGACTCAATTACCCTTTCTAAAGAAAAAAGAAAAAAAAAAGAAAAAAGAAATAACGCCTGTTCTTTCTGTATCTTCTCTCTCAGCATCTCTGTGTTGCTTTACGTTCCCAACAACTTGGCATCGGCCATTGCTCCCTTATTTATATTATTACCAACAATGAGCTTCACCGCTGCAACTCCAATCCATACGTCGTCGTATCCCCCTTCTTCTCTCCATTCCAATCGCTTCAACCCCACAATCCTCTCTCCCATCCCTCTCCGCCGTTCCTTTTCTTCTTTCATCCCCCGCCGCACCCCTCCTCTGCTTTCCGCTTCAGCCACGACGCCCGCAGACATCCAGATGGACGCCGATCGCCTTCAGAAGGGAATCGCCGAGTTCTACGATGAATCATCGGGGCTTTGGGAGAGCGTTTGGGGCGAACATATGCACCATGGATTTTACGATCCTGATTCTTCCATTTCCCTCTCCGATCACCGCTCTGCGCAAATCCGGATGATCGAGGAGACGCTCCGGTTCGCCGGCGTGGAGGCGGCGACGGCAGCGGTGGACGTTGGGTGTGGGATTGGGGGAAGTTCTAGGTATTTGGCGAAGAAGTTTGGGGCGAAATGTAGAGGTATAACTTTGAGCCCTGTCCAAGCTAAACGCGCTCAAGCGATTGCTGCCGCTGAAGGATTAAGTGATAAGGTGTGTTTTGAAGTGGCAGATGCTCTGAACCAACCATTCTCAGATGGGGAATTTGATCTGGTATGGTCCATGGAGAGTGGGGAGCACATGCCTGACAAATCCAAGTTTGTGAGTGAACTAGTTAGAGTTGCAGCCCCAGGAAGTACCATAATTATAGTTACATGGTGCCATAGAAACCTTGGGCCTTCTGAAAACTCCTTGCAGCCATGGGAGGAGAAGCTCCTTCAGAAGATTTGTGATGGCTTCTATCTCCCTGCTTGGTGTTCTACTGCTGATTATGTCAAATTACTCGAATCCCACAATCTACAGGATATAAAGACAGCAGACTGGTCTCAGAATGTTGCTCCGTTTTGGCCTGCAGTTATTCGGTCTGCTTTGACTTGGAAAGGCTTCACATCACTCTTACGAAGTGGTAAATATTCCTGACACTAAAAAATAATAATAAAAAAAAATCAATTTTACTCAACTGAAAATAAAATAAAATAATCAATTTTTTGTTAAAAACTTTTCCAAAATTCCTTTCTCAAACCACACTTTACTTGTTATTGCTTTTAATGGTCTAAATTTAAATTATGTGATTTTATTAAATCATATTGAAATTGATTAAATAATAATAATAGAAGAAAAAAAAGTATGTAATATATTTTTAAAATTTATAAAAAGAAAGTTAATAGGGACTGATTTTTTTTTCTTATAAAAAAACTAAAAATAAATTAGGAGTAG

Coding sequence (CDS)

ATGAGCTTCACCGCTGCAACTCCAATCCATACGTCGTCGTATCCCCCTTCTTCTCTCCATTCCAATCGCTTCAACCCCACAATCCTCTCTCCCATCCCTCTCCGCCGTTCCTTTTCTTCTTTCATCCCCCGCCGCACCCCTCCTCTGCTTTCCGCTTCAGCCACGACGCCCGCAGACATCCAGATGGACGCCGATCGCCTTCAGAAGGGAATCGCCGAGTTCTACGATGAATCATCGGGGCTTTGGGAGAGCGTTTGGGGCGAACATATGCACCATGGATTTTACGATCCTGATTCTTCCATTTCCCTCTCCGATCACCGCTCTGCGCAAATCCGGATGATCGAGGAGACGCTCCGGTTCGCCGGCGTGGAGGCGGCGACGGCAGCGGTGGACGTTGGGTGTGGGATTGGGGGAAGTTCTAGGTATTTGGCGAAGAAGTTTGGGGCGAAATGTAGAGGTATAACTTTGAGCCCTGTCCAAGCTAAACGCGCTCAAGCGATTGCTGCCGCTGAAGGATTAAGTGATAAGGTGTGTTTTGAAGTGGCAGATGCTCTGAACCAACCATTCTCAGATGGGGAATTTGATCTGGTATGGTCCATGGAGAGTGGGGAGCACATGCCTGACAAATCCAAGTTTGTGAGTGAACTAGTTAGAGTTGCAGCCCCAGGAAGTACCATAATTATAGTTACATGGTGCCATAGAAACCTTGGGCCTTCTGAAAACTCCTTGCAGCCATGGGAGGAGAAGCTCCTTCAGAAGATTTGTGATGGCTTCTATCTCCCTGCTTGGTGTTCTACTGCTGATTATGTCAAATTACTCGAATCCCACAATCTACAGGATATAAAGACAGCAGACTGGTCTCAGAATGTTGCTCCGTTTTGGCCTGCAGTTATTCGGTCTGCTTTGACTTGGAAAGGCTTCACATCACTCTTACGAAGTGGTAAATATTCCTGA

Protein sequence

MSFTAATPIHTSSYPPSSLHSNRFNPTILSPIPLRRSFSSFIPRRTPPLLSASATTPADIQMDADRLQKGIAEFYDESSGLWESVWGEHMHHGFYDPDSSISLSDHRSAQIRMIEETLRFAGVEAATAAVDVGCGIGGSSRYLAKKFGAKCRGITLSPVQAKRAQAIAAAEGLSDKVCFEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGSTIIIVTWCHRNLGPSENSLQPWEEKLLQKICDGFYLPAWCSTADYVKLLESHNLQDIKTADWSQNVAPFWPAVIRSALTWKGFTSLLRSGKYS

Homology

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BLAST of Lsi10G002240 vs. Swiss-Prot
Match: GTOMC_ARATH (Tocopherol O-methyltransferase, chloroplastic OS=Arabidopsis thaliana GN=VTE4 PE=1 SV=2)

HSP 1 Score: 405.6 bits (1041), Expect = 5.0e-112
Identity = 207/325 (63.69%), Postives = 240/325 (73.85%), Query Frame = 1


Query: 1   MSFTAATPIHTSSYPPSSLHSNRFNPTILSPIPLRRSFSSFIPRRTPPLLSASATTPADI 60
           M  T A P   +S P  +  S     ++L   P   S  S    R    ++A+AT+    
Sbjct: 1   MKATLAAPSSLTSLPYRTNSSFGSKSSLLFRSPSSSSSVSMTTTRGNVAVAAAATS---- 60

Query: 61  QMDADRLQKGIAEFYDESSGLWESVWGEHMHHGFYDPDSSISLSD--HRSAQIRMIEETL 120
               + L+KGIAEFY+E+SGLWE +WG+HMHHGFYDPDSS+ LSD  H+ AQIRMIEE+L
Sbjct: 61  ---TEALRKGIAEFYNETSGLWEEIWGDHMHHGFYDPDSSVQLSDSGHKEAQIRMIEESL 120

Query: 121 RFAGV------EAATAAVDVGCGIGGSSRYLAKKFGAKCRGITLSPVQAKRAQAIAAAEG 180
           RFAGV      +     VDVGCGIGGSSRYLA KFGA+C GITLSPVQAKRA  +AAA+ 
Sbjct: 121 RFAGVTDEEEEKKIKKVVDVGCGIGGSSRYLASKFGAECIGITLSPVQAKRANDLAAAQS 180

Query: 181 LSDKVCFEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGSTIIIVTWC 240
           L+ K  F+VADAL+QPF DG+FDLVWSMESGEHMPDK+KFV ELVRVAAPG  IIIVTWC
Sbjct: 181 LAHKASFQVADALDQPFEDGKFDLVWSMESGEHMPDKAKFVKELVRVAAPGGRIIIVTWC 240

Query: 241 HRNLGPSENSLQPWEEKLLQKICDGFYLPAWCSTADYVKLLESHNLQDIKTADWSQNVAP 300
           HRNL   E +LQPWE+ +L KIC  FYLPAWCST DYV LL+SH+LQDIK ADWS+NVAP
Sbjct: 241 HRNLSAGEEALQPWEQNILDKICKTFYLPAWCSTDDYVNLLQSHSLQDIKCADWSENVAP 300

Query: 301 FWPAVIRSALTWKGFTSLLRSGKYS 318
           FWPAVIR+ALTWKG  SLLRSG  S
Sbjct: 301 FWPAVIRTALTWKGLVSLLRSGMKS 318

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BLAST of Lsi10G002240 vs. Swiss-Prot
Match: GTOMC_ORYSJ (Probable tocopherol O-methyltransferase, chloroplastic OS=Oryza sativa subsp. japonica GN=VTE4 PE=2 SV=1)

HSP 1 Score: 389.4 bits (999), Expect = 3.7e-107
Identity = 190/276 (68.84%), Postives = 221/276 (80.07%), Query Frame = 1


Query: 44  RRTPPLLSASATTPADIQMDADRLQKGIAEFYDESSGLWESVWGEHMHHGFYDPDSSISL 103
           R   P LS+S+T  A        L++GIA  YDESSG+WES+WGEHMHHGFYD   + S+
Sbjct: 55  RAMAPTLSSSSTAAAA----PPGLKEGIAGLYDESSGVWESIWGEHMHHGFYDAGEAASM 114

Query: 104 SDHRSAQIRMIEETLRFAGV-----EAATAAVDVGCGIGGSSRYLAKKFGAKCRGITLSP 163
           SDHR AQIRMIEE+L FA V     +   + VDVGCGIGGSSRYLA K+GA+C GITLSP
Sbjct: 115 SDHRRAQIRMIEESLAFAAVPDDAEKKPKSVVDVGCGIGGSSRYLANKYGAQCYGITLSP 174

Query: 164 VQAKRAQAIAAAEGLSDKVCFEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVR 223
           VQA+R  A+AA +GLSDKV F+V DAL QPF DG+FDLVWSMESGEHMPDK +FVSEL R
Sbjct: 175 VQAERGNALAAEQGLSDKVSFQVGDALEQPFPDGQFDLVWSMESGEHMPDKRQFVSELAR 234

Query: 224 VAAPGSTIIIVTWCHRNLGPSENSLQPWEEKLLQKICDGFYLPAWCSTADYVKLLESHNL 283
           VAAPG+ IIIVTWCHRNL PSE SL+P E  LL++ICD +YLP WCS +DYVK+ ES +L
Sbjct: 235 VAAPGARIIIVTWCHRNLEPSEESLKPDELNLLKRICDAYYLPDWCSPSDYVKIAESLSL 294

Query: 284 QDIKTADWSQNVAPFWPAVIRSALTWKGFTSLLRSG 315
           +DI+TADWS+NVAPFWPAVI+SALTWKG TSLLRSG
Sbjct: 295 EDIRTADWSENVAPFWPAVIKSALTWKGLTSLLRSG 326

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BLAST of Lsi10G002240 vs. Swiss-Prot
Match: BQMT_SYNY3 (2-methyl-6-phytyl-1,4-hydroquinone methyltransferase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) GN=sll0418 PE=1 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 2.2e-35
Identity = 88/244 (36.07%), Postives = 133/244 (54.51%), Query Frame = 1


Query: 71  IAEFYDE--SSGLWESVWGEHMHHGFY-DPDSSISLSDHRSAQIRMIEETLRFAGVEA-- 130
           +A  YD+    G+ E  WG+H+H G Y DP  +    D   ++I  +    ++ G++   
Sbjct: 36  VANAYDQWTEDGILEYYWGDHIHLGHYGDPPVA---KDFIQSKIDFVHAMAQWGGLDTLP 95

Query: 131 -ATAAVDVGCGIGGSSRYLAKKFGAKCRGITLSPVQAKRAQAIAAAEGLSDKVCFEVADA 190
             T  +DVGCGIGGSSR LAK +G    GIT+SP Q KRA  +   +  +    F V DA
Sbjct: 96  PGTTVLDVGCGIGGSSRILAKDYGFNVTGITISPQQVKRATELTPPDVTAK---FAVDDA 155

Query: 191 LNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGSTIIIVTWCHRNLGPSENSLQ 250
           +   F DG FD+VWS+E+G HMPDK+ F  EL+RV  PG  +++  W  R+    +  L 
Sbjct: 156 MALSFPDGSFDVVWSVEAGPHMPDKAVFAKELLRVVKPGGILVVADWNQRD--DRQVPLN 215

Query: 251 PWEEKLLQKICDGFYLPAWCSTADYVKLLESHNLQD--IKTADWSQNVAPFWPAVIRSAL 307
            WE+ +++++ D +  PA+ S   + + LE+  L +  + TADW+    P W   I    
Sbjct: 216 FWEKPVMRQLLDQWSHPAFASIEGFAENLEATGLVEGQVTTADWTVPTLPAWLDTI---- 266

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BLAST of Lsi10G002240 vs. Swiss-Prot
Match: REBMT_NOCAE (Demethylrebeccamycin-D-glucose O-methyltransferase OS=Lechevalieria aerocolonigenes GN=rebM PE=1 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 5.1e-24
Identity = 58/159 (36.48%), Postives = 93/159 (58.49%), Query Frame = 1


Query: 71  IAEFYDESSGLWESVWGEHMHHGFY-DPDSSISLSDHRSAQIRMIEETLRFAGVEAATAA 130
           + + YD+ +  +  +WGE++H G++ D  + +S+ D   A  R+ +E +    V +    
Sbjct: 19  VRQMYDDFTDPFARIWGENLHFGYWEDAGADVSVDD---ATDRLTDEMIALLDVRSGDRV 78

Query: 131 VDVGCGIGGSSRYLAKKFGAKCRGITLSPVQAKRAQAIAAAEGLSDKVCFEVADALNQPF 190
           +DVGCGIG  +  LA     +  GI++S  Q  +A A A A GL+++V F  ADA++ PF
Sbjct: 79  LDVGCGIGKPAVRLATARDVRVTGISISRPQVNQANARATAAGLANRVTFSYADAMDLPF 138

Query: 191 SDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGSTIII 229
            D  FD VW++ES  HMPD+ + + E+ RV  PG T+ I
Sbjct: 139 EDASFDAVWALESLHHMPDRGRALREMARVLRPGGTVAI 174

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BLAST of Lsi10G002240 vs. Swiss-Prot
Match: DMRMT_AMYMS (27-O-demethylrifamycin SV methyltransferase OS=Amycolatopsis mediterranei (strain S699) GN=RAM_03320 PE=1 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 2.8e-22
Identity = 69/224 (30.80%), Postives = 109/224 (48.66%), Query Frame = 1


Query: 71  IAEFYDESSGLWESVWGEHMHHGFYDPDSSISLSDHRSAQIRMIEETLRFAGVEAATAAV 130
           I + YD  + L + +WGE++HHG++D D S +L +   A  R+ +       + A    +
Sbjct: 9   IGKGYDAFADLLDQLWGENLHHGYWD-DESATLEE---ATTRLTDRLAGMLPLRAGDRLL 68

Query: 131 DVGCGIGGSSRYLAKKFGAKCRGITLSPVQAKRAQAIAAAEGLSDKVCFEVADALNQPFS 190
           D+GCG G  +  +A        GI++S  Q +RA   A    + D+V FE ADA+  P+ 
Sbjct: 69  DIGCGNGEPAIRMATANDVMVTGISISEKQVERANDRAYKADVDDRVVFEYADAMELPYP 128

Query: 191 DGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGSTIIIVTWCHRNLGPSENSLQPWEEKL 250
           D  FD+VW++ES  HMPD+   + +  RV  PG  + +  +    L PS   L+   E++
Sbjct: 129 DASFDVVWALESLHHMPDRWHVIRQAARVLRPGGRLALGDFL---LVPSPAGLEADAERV 188

Query: 251 LQKICDGFYLPAWCSTADYVKLLESHNLQDIKTADWSQNVAPFW 295
            +    G  + A  S  +Y   L    L+     D SQ   P W
Sbjct: 189 REV---GKGVVAVVSLDEYQAHLREAGLEPESAEDVSQYTRPSW 222

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BLAST of Lsi10G002240 vs. TrEMBL
Match: A0A0A0LTM1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G432780 PE=4 SV=1)

HSP 1 Score: 548.9 bits (1413), Expect = 4.1e-153
Identity = 271/316 (85.76%), Postives = 288/316 (91.14%), Query Frame = 1


Query: 1   MSFTAATPIHTSSYPPSSLHSNRFNPTILSPIPLRRSFSSFIPRRTPPLLSAS--ATTPA 60
           M+ TAATPIHTSSYPPSSLHSN FN   +SP P   SFSSF PRR  PLL++S  ATTP 
Sbjct: 1   MNLTAATPIHTSSYPPSSLHSNPFNS--ISPRP---SFSSFTPRRFSPLLASSSAATTPP 60

Query: 61  DIQMDADRLQKGIAEFYDESSGLWESVWGEHMHHGFYDPDSSISLSDHRSAQIRMIEETL 120
            + MD+ RL KGIAEFYD+SSGLWE+VWG+HMHHGFYDP SS+SLSDHR+AQIRMIEETL
Sbjct: 61  SLHMDSHRLHKGIAEFYDQSSGLWENVWGQHMHHGFYDPHSSVSLSDHRAAQIRMIEETL 120

Query: 121 RFAGVEAATAAVDVGCGIGGSSRYLAKKFGAKCRGITLSPVQAKRAQAIAAAEGLSDKVC 180
           RFAGVEAATA VDVGCGIGGSSRYLA KFGAKCRGITLSPVQAKRAQ I+AAEGLSDKVC
Sbjct: 121 RFAGVEAATAVVDVGCGIGGSSRYLATKFGAKCRGITLSPVQAKRAQEISAAEGLSDKVC 180

Query: 181 FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGSTIIIVTWCHRNLGP 240
           FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRV APG TIIIVTWCHR+LGP
Sbjct: 181 FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVTAPGGTIIIVTWCHRDLGP 240

Query: 241 SENSLQPWEEKLLQKICDGFYLPAWCSTADYVKLLESHNLQDIKTADWSQNVAPFWPAVI 300
           SE+SLQPWE KLLQKICDGFYLPAWCSTADYVKLL+SHNLQDIK ADWS+NVAPFWPAVI
Sbjct: 241 SESSLQPWELKLLQKICDGFYLPAWCSTADYVKLLQSHNLQDIKRADWSENVAPFWPAVI 300

Query: 301 RSALTWKGFTSLLRSG 315
           RSA TWKGFTSLLR+G
Sbjct: 301 RSAFTWKGFTSLLRTG 311

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BLAST of Lsi10G002240 vs. TrEMBL
Match: V4SS85_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10032026mg PE=4 SV=1)

HSP 1 Score: 434.5 bits (1116), Expect = 1.1e-118
Identity = 208/273 (76.19%), Postives = 233/273 (85.35%), Query Frame = 1


Query: 52  ASATTPADIQMDAD--RLQKGIAEFYDESSGLWESVWGEHMHHGFYDPDSSISLSDHRSA 111
           AS+TT      DA    L++GIAEFYDESS LWE +WG+HMHHGFY+PDSS+S+SDHR+A
Sbjct: 38  ASSTTSTTTMSDAAARELKEGIAEFYDESSSLWEDIWGDHMHHGFYEPDSSVSVSDHRAA 97

Query: 112 QIRMIEETLRFAGVEAATA-----AVDVGCGIGGSSRYLAKKFGAKCRGITLSPVQAKRA 171
           Q+RMIEETLRFAGV           VDVGCGIGGSSRYLAKKFGAKC+GITLSPVQA+RA
Sbjct: 98  QVRMIEETLRFAGVSEDPTKRPKNVVDVGCGIGGSSRYLAKKFGAKCQGITLSPVQAQRA 157

Query: 172 QAIAAAEGLSDKVCFEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGS 231
            A+AAA GL+DKV F+V DAL QPF DG+FDLVWSMESGEHMPDKSKFVSEL RV AP  
Sbjct: 158 NALAAARGLADKVSFQVGDALQQPFPDGQFDLVWSMESGEHMPDKSKFVSELARVTAPAG 217

Query: 232 TIIIVTWCHRNLGPSENSLQPWEEKLLQKICDGFYLPAWCSTADYVKLLESHNLQDIKTA 291
           TIIIVTWCHR+L PSE SLQPWE++LL+KICD +YLPAWCSTADYVKLL+S +L+DIK  
Sbjct: 218 TIIIVTWCHRDLAPSEESLQPWEQELLKKICDAYYLPAWCSTADYVKLLQSLSLEDIKAE 277

Query: 292 DWSQNVAPFWPAVIRSALTWKGFTSLLRSGKYS 318
           DWSQNVAPFWPAVIRSALTWKGFTSLLR+GK S
Sbjct: 278 DWSQNVAPFWPAVIRSALTWKGFTSLLRTGKLS 310

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BLAST of Lsi10G002240 vs. TrEMBL
Match: A0A067DYF3_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g016921mg PE=4 SV=1)

HSP 1 Score: 432.6 bits (1111), Expect = 4.2e-118
Identity = 207/273 (75.82%), Postives = 232/273 (84.98%), Query Frame = 1


Query: 52  ASATTPADIQMDAD--RLQKGIAEFYDESSGLWESVWGEHMHHGFYDPDSSISLSDHRSA 111
           AS+TT      DA    L++GIAEFYDESS LWE +WG+HMHHGFY+PDSS+S+SDHR+A
Sbjct: 78  ASSTTSTTTMSDAAARELKEGIAEFYDESSSLWEDIWGDHMHHGFYEPDSSVSVSDHRAA 137

Query: 112 QIRMIEETLRFAGVEAATA-----AVDVGCGIGGSSRYLAKKFGAKCRGITLSPVQAKRA 171
           Q+RMIEETLRFAGV           VDVGCGIGGSSRYLAKKFGAKC+GITLSPVQA+RA
Sbjct: 138 QVRMIEETLRFAGVSEDPTKRPKNVVDVGCGIGGSSRYLAKKFGAKCQGITLSPVQAQRA 197

Query: 172 QAIAAAEGLSDKVCFEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGS 231
            A+AAA GL+DKV F+V DAL QPF DG+FDLVWSMESGEHMPDKSKFVSEL RV AP  
Sbjct: 198 NALAAARGLADKVSFQVGDALQQPFPDGQFDLVWSMESGEHMPDKSKFVSELARVTAPAG 257

Query: 232 TIIIVTWCHRNLGPSENSLQPWEEKLLQKICDGFYLPAWCSTADYVKLLESHNLQDIKTA 291
           TIIIVTWCHR+L PSE SLQPWE++LL+KICD +YLPAWCSTADYVKLL+S +L+DIK  
Sbjct: 258 TIIIVTWCHRDLAPSEESLQPWEQELLKKICDAYYLPAWCSTADYVKLLQSLSLEDIKAE 317

Query: 292 DWSQNVAPFWPAVIRSALTWKGFTSLLRSGKYS 318
           DWSQNVAPFWPAVI SALTWKGFTSLLR+GK S
Sbjct: 318 DWSQNVAPFWPAVIHSALTWKGFTSLLRTGKLS 350

[Close]

BLAST of Lsi10G002240 vs. TrEMBL
Match: V4TBP0_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10005360mg PE=4 SV=1)

HSP 1 Score: 431.4 bits (1108), Expect = 9.4e-118
Identity = 206/270 (76.30%), Postives = 231/270 (85.56%), Query Frame = 1


Query: 52  ASATTPADIQMDAD--RLQKGIAEFYDESSGLWESVWGEHMHHGFYDPDSSISLSDHRSA 111
           AS+TT      DA    L++GIAEFYDESS LWE +WG+HMHHGFY+PDSS+S+SDHR+A
Sbjct: 38  ASSTTSTTTMSDAAARELKEGIAEFYDESSSLWEDIWGDHMHHGFYEPDSSVSVSDHRAA 97

Query: 112 QIRMIEETLRFAGVEAATA-----AVDVGCGIGGSSRYLAKKFGAKCRGITLSPVQAKRA 171
           Q+RMIEETLRFAGV           VDVGCGIGGSSRYLAKKFGAKC+GITLSPVQA+RA
Sbjct: 98  QVRMIEETLRFAGVSEDPTKRPKNVVDVGCGIGGSSRYLAKKFGAKCQGITLSPVQAQRA 157

Query: 172 QAIAAAEGLSDKVCFEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGS 231
            A+AAA GL+DKV F+V DAL QPF DG+FDLVWSMESGEHMPDKSKFVSEL RV AP  
Sbjct: 158 NALAAARGLADKVSFQVGDALQQPFPDGQFDLVWSMESGEHMPDKSKFVSELARVTAPAG 217

Query: 232 TIIIVTWCHRNLGPSENSLQPWEEKLLQKICDGFYLPAWCSTADYVKLLESHNLQDIKTA 291
           TIIIVTWCHR+L PSE SLQPWE++LL+KICD +YLPAWCSTADYVKLL+S +L+DIK  
Sbjct: 218 TIIIVTWCHRDLAPSEESLQPWEQELLKKICDAYYLPAWCSTADYVKLLQSLSLEDIKAE 277

Query: 292 DWSQNVAPFWPAVIRSALTWKGFTSLLRSG 315
           DWSQNVAPFWPAVIRSALTWKGFTSLLR+G
Sbjct: 278 DWSQNVAPFWPAVIRSALTWKGFTSLLRTG 307

[Close]

BLAST of Lsi10G002240 vs. TrEMBL
Match: A0A067EAQ8_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g016921mg PE=4 SV=1)

HSP 1 Score: 429.5 bits (1103), Expect = 3.6e-117
Identity = 205/270 (75.93%), Postives = 230/270 (85.19%), Query Frame = 1


Query: 52  ASATTPADIQMDAD--RLQKGIAEFYDESSGLWESVWGEHMHHGFYDPDSSISLSDHRSA 111
           AS+TT      DA    L++GIAEFYDESS LWE +WG+HMHHGFY+PDSS+S+SDHR+A
Sbjct: 78  ASSTTSTTTMSDAAARELKEGIAEFYDESSSLWEDIWGDHMHHGFYEPDSSVSVSDHRAA 137

Query: 112 QIRMIEETLRFAGVEAATA-----AVDVGCGIGGSSRYLAKKFGAKCRGITLSPVQAKRA 171
           Q+RMIEETLRFAGV           VDVGCGIGGSSRYLAKKFGAKC+GITLSPVQA+RA
Sbjct: 138 QVRMIEETLRFAGVSEDPTKRPKNVVDVGCGIGGSSRYLAKKFGAKCQGITLSPVQAQRA 197

Query: 172 QAIAAAEGLSDKVCFEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGS 231
            A+AAA GL+DKV F+V DAL QPF DG+FDLVWSMESGEHMPDKSKFVSEL RV AP  
Sbjct: 198 NALAAARGLADKVSFQVGDALQQPFPDGQFDLVWSMESGEHMPDKSKFVSELARVTAPAG 257

Query: 232 TIIIVTWCHRNLGPSENSLQPWEEKLLQKICDGFYLPAWCSTADYVKLLESHNLQDIKTA 291
           TIIIVTWCHR+L PSE SLQPWE++LL+KICD +YLPAWCSTADYVKLL+S +L+DIK  
Sbjct: 258 TIIIVTWCHRDLAPSEESLQPWEQELLKKICDAYYLPAWCSTADYVKLLQSLSLEDIKAE 317

Query: 292 DWSQNVAPFWPAVIRSALTWKGFTSLLRSG 315
           DWSQNVAPFWPAVI SALTWKGFTSLLR+G
Sbjct: 318 DWSQNVAPFWPAVIHSALTWKGFTSLLRTG 347

[Close]

BLAST of Lsi10G002240 vs. TAIR10
Match: AT1G64970.1 (AT1G64970.1 gamma-tocopherol methyltransferase)

HSP 1 Score: 405.6 bits (1041), Expect = 2.8e-113
Identity = 207/325 (63.69%), Postives = 240/325 (73.85%), Query Frame = 1


Query: 1   MSFTAATPIHTSSYPPSSLHSNRFNPTILSPIPLRRSFSSFIPRRTPPLLSASATTPADI 60
           M  T A P   +S P  +  S     ++L   P   S  S    R    ++A+AT+    
Sbjct: 1   MKATLAAPSSLTSLPYRTNSSFGSKSSLLFRSPSSSSSVSMTTTRGNVAVAAAATS---- 60

Query: 61  QMDADRLQKGIAEFYDESSGLWESVWGEHMHHGFYDPDSSISLSD--HRSAQIRMIEETL 120
               + L+KGIAEFY+E+SGLWE +WG+HMHHGFYDPDSS+ LSD  H+ AQIRMIEE+L
Sbjct: 61  ---TEALRKGIAEFYNETSGLWEEIWGDHMHHGFYDPDSSVQLSDSGHKEAQIRMIEESL 120

Query: 121 RFAGV------EAATAAVDVGCGIGGSSRYLAKKFGAKCRGITLSPVQAKRAQAIAAAEG 180
           RFAGV      +     VDVGCGIGGSSRYLA KFGA+C GITLSPVQAKRA  +AAA+ 
Sbjct: 121 RFAGVTDEEEEKKIKKVVDVGCGIGGSSRYLASKFGAECIGITLSPVQAKRANDLAAAQS 180

Query: 181 LSDKVCFEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGSTIIIVTWC 240
           L+ K  F+VADAL+QPF DG+FDLVWSMESGEHMPDK+KFV ELVRVAAPG  IIIVTWC
Sbjct: 181 LAHKASFQVADALDQPFEDGKFDLVWSMESGEHMPDKAKFVKELVRVAAPGGRIIIVTWC 240

Query: 241 HRNLGPSENSLQPWEEKLLQKICDGFYLPAWCSTADYVKLLESHNLQDIKTADWSQNVAP 300
           HRNL   E +LQPWE+ +L KIC  FYLPAWCST DYV LL+SH+LQDIK ADWS+NVAP
Sbjct: 241 HRNLSAGEEALQPWEQNILDKICKTFYLPAWCSTDDYVNLLQSHSLQDIKCADWSENVAP 300

Query: 301 FWPAVIRSALTWKGFTSLLRSGKYS 318
           FWPAVIR+ALTWKG  SLLRSG  S
Sbjct: 301 FWPAVIRTALTWKGLVSLLRSGMKS 318

[Close]

BLAST of Lsi10G002240 vs. TAIR10
Match: AT1G20330.1 (AT1G20330.1 sterol methyltransferase 2)

HSP 1 Score: 82.0 bits (201), Expect = 7.1e-16
Identity = 66/240 (27.50%), Postives = 108/240 (45.00%), Query Frame = 1


Query: 57  PADIQMDADRLQKGIAEFYDESSGLWESVWGEHMHHGFYDPDSSISLSDHRSAQIRMIEE 116
           P +I+  A+++   +  FY+  + ++E  WG+  H   + P  SI    H+ A     E 
Sbjct: 61  PKEIET-AEKVPDFVDTFYNLVTDIYEWGWGQSFH---FSP--SIPGKSHKDATRLHEEM 120

Query: 117 TLRFAGVEAATAAVDVGCGIGGSSRYLAKKFGAKCRGITLSPVQAKRAQAIAAAEGLSDK 176
            +    V+     +DVGCG+GG  R +A    A   GIT++  Q  RA+      GL D 
Sbjct: 121 AVDLIQVKPGQKILDVGCGVGGPMRAIASHSRANVVGITINEYQVNRARLHNKKAGL-DA 180

Query: 177 VCFEV-ADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGSTIIIVTWCHRN 236
           +C  V  + L  PF D  FD  +S+E+  H P   +  +E+ RV  PGS  +   W    
Sbjct: 181 LCEVVCGNFLQMPFDDNSFDGAYSIEATCHAPKLEEVYAEIYRVLKPGSMYVSYEWVTTE 240

Query: 237 LGPSENSLQPWEEKLLQKICDGFYLPAWCSTADYVKLLESHNLQDIKTADWSQNVA-PFW 295
              +E+       +++Q I  G  LP   +  D  +  +    + +K  D +   A P+W
Sbjct: 241 KFKAEDD---EHVEVIQGIERGDALPGLRAYVDIAETAKKVGFEIVKEKDLASPPAEPWW 290

[Close]

BLAST of Lsi10G002240 vs. TAIR10
Match: AT1G76090.1 (AT1G76090.1 sterol methyltransferase 3)

HSP 1 Score: 78.6 bits (192), Expect = 7.9e-15
Identity = 64/240 (26.67%), Postives = 109/240 (45.42%), Query Frame = 1


Query: 57  PADIQMDADRLQKGIAEFYDESSGLWESVWGEHMHHGFYDPDSSISLSDHRSAQIRMIEE 116
           P +I+  A+++   +  FY+  + ++E  WG+  H   + P  S    D  + +I   E 
Sbjct: 61  PKEIE-SAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPHVPGKS----DKDATRIHE-EM 120

Query: 117 TLRFAGVEAATAAVDVGCGIGGSSRYLAKKFGAKCRGITLSPVQAKRAQAIAAAEGLSDK 176
            +    V+     +D GCG+GG  R +A    A+  GIT++  Q +RA+      GL D 
Sbjct: 121 AVDLIKVKPGQKILDAGCGVGGPMRAIAAHSKAQVTGITINEYQVQRAKLHNKKAGL-DS 180

Query: 177 VCFEV-ADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGSTIIIVTWCHRN 236
           +C  V  + L  PF +  FD  +S+E+  H P   +  SE+ RV  PGS  +   W    
Sbjct: 181 LCNVVCGNFLKMPFDENTFDGAYSIEATCHAPKLEEVYSEIFRVMKPGSLFVSYEWVTTE 240

Query: 237 LGPSENSLQPWEEKLLQKICDGFYLPAWCSTADYVKLLESHNLQDIKTADWSQNVA-PFW 295
               ++      + ++Q I  G  LP   S AD     +    + +K  D ++  + P+W
Sbjct: 241 KYRDDDE---EHKDVIQGIERGDALPGLRSYADIAVTAKKVGFEVVKEKDLAKPPSKPWW 290

[Close]

BLAST of Lsi10G002240 vs. TAIR10
Match: AT4G33110.1 (AT4G33110.1 S-adenosyl-L-methionine-dependent methyltransferases superfamily protein)

HSP 1 Score: 51.6 bits (122), Expect = 1.0e-06
Identity = 47/171 (27.49%), Postives = 85/171 (49.71%), Query Frame = 1


Query: 68  QKGIAEFYDESSGLWESVWGEHMHHG--FYDPDSSISLSDHRSAQIRMIEETLRFAGVEA 127
           +K   + Y+  +  +E V G +M +   ++  DSS SL D   A + +  E    A VE 
Sbjct: 74  EKPKTQHYELPTAFFELVLGRNMKYSSCYFSNDSS-SLEDAEEAILALYCER---AKVED 133

Query: 128 ATAAVDVGCGIGGSSRYLAKKFGAKCR--GITLSPVQAKRAQAIAAAEGLSDKVCFEVAD 187
             + +D+GCG G  S Y+A+K+ +KC+  GI  S  Q           GL + +   VAD
Sbjct: 134 GQSVLDIGCGWGSLSLYIARKY-SKCKLTGICNSKTQKAFIDEKCRKLGLQN-IEIIVAD 193

Query: 188 ALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGSTIIIVTWCHR 235
            ++    +G +D ++S+E  EHM +  + + ++ +     S + +  +CH+
Sbjct: 194 -ISTFEHEGTYDRIFSIEMFEHMKNYGELLKKIGKWMKEDSLLFVHYFCHK 237

[Close]

BLAST of Lsi10G002240 vs. NCBI nr
Match: gi|659125635|ref|XP_008462787.1| (PREDICTED: tocopherol O-methyltransferase, chloroplastic [Cucumis melo])

HSP 1 Score: 566.6 bits (1459), Expect = 2.7e-158
Identity = 276/314 (87.90%), Postives = 294/314 (93.63%), Query Frame = 1


Query: 1   MSFTAATPIHTSSYPPSSLHSNRFNPTILSPIPLRRSFSSFIPRRTPPLLSASATTPADI 60
           M+FTAATPIHTS YPPSSLH NR NPTIL+PI LR SFSSF PRR+ P L+ +ATT +D 
Sbjct: 1   MNFTAATPIHTSFYPPSSLHFNRLNPTILNPISLRPSFSSFTPRRSSPFLAFAATTASD- 60

Query: 61  QMDADRLQKGIAEFYDESSGLWESVWGEHMHHGFYDPDSSISLSDHRSAQIRMIEETLRF 120
            MD+ RLQKGIAEFYD+SSGLWE+VWGEHMHHGFYDPDSS+SLSDHR+AQ+RMIEETLRF
Sbjct: 61  HMDSHRLQKGIAEFYDQSSGLWENVWGEHMHHGFYDPDSSVSLSDHRAAQVRMIEETLRF 120

Query: 121 AGVEAATAAVDVGCGIGGSSRYLAKKFGAKCRGITLSPVQAKRAQAIAAAEGLSDKVCFE 180
           AGVE ATA VDVGCGIGGSSRYLA+KFGAKCRGITLSPVQAKRAQAIAAAEGLSDKV FE
Sbjct: 121 AGVEEATAVVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAAAEGLSDKVYFE 180

Query: 181 VADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGSTIIIVTWCHRNLGPSE 240
           VADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPG+TIIIVTWCHR+LGPSE
Sbjct: 181 VADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLGPSE 240

Query: 241 NSLQPWEEKLLQKICDGFYLPAWCSTADYVKLLESHNLQDIKTADWSQNVAPFWPAVIRS 300
           +SLQPWE KLLQKICDGFYLPAWCSTADYVKLL+SHNLQDIK ADWS NVAPFWPAVIRS
Sbjct: 241 SSLQPWELKLLQKICDGFYLPAWCSTADYVKLLQSHNLQDIKRADWSGNVAPFWPAVIRS 300

Query: 301 ALTWKGFTSLLRSG 315
           A TWKGFTSLLR+G
Sbjct: 301 AFTWKGFTSLLRTG 313

[Close]

BLAST of Lsi10G002240 vs. NCBI nr
Match: gi|778673784|ref|XP_004151846.2| (PREDICTED: tocopherol O-methyltransferase, chloroplastic [Cucumis sativus])

HSP 1 Score: 548.9 bits (1413), Expect = 5.8e-153
Identity = 271/316 (85.76%), Postives = 288/316 (91.14%), Query Frame = 1


Query: 1   MSFTAATPIHTSSYPPSSLHSNRFNPTILSPIPLRRSFSSFIPRRTPPLLSAS--ATTPA 60
           M+ TAATPIHTSSYPPSSLHSN FN   +SP P   SFSSF PRR  PLL++S  ATTP 
Sbjct: 1   MNLTAATPIHTSSYPPSSLHSNPFNS--ISPRP---SFSSFTPRRFSPLLASSSAATTPP 60

Query: 61  DIQMDADRLQKGIAEFYDESSGLWESVWGEHMHHGFYDPDSSISLSDHRSAQIRMIEETL 120
            + MD+ RL KGIAEFYD+SSGLWE+VWG+HMHHGFYDP SS+SLSDHR+AQIRMIEETL
Sbjct: 61  SLHMDSHRLHKGIAEFYDQSSGLWENVWGQHMHHGFYDPHSSVSLSDHRAAQIRMIEETL 120

Query: 121 RFAGVEAATAAVDVGCGIGGSSRYLAKKFGAKCRGITLSPVQAKRAQAIAAAEGLSDKVC 180
           RFAGVEAATA VDVGCGIGGSSRYLA KFGAKCRGITLSPVQAKRAQ I+AAEGLSDKVC
Sbjct: 121 RFAGVEAATAVVDVGCGIGGSSRYLATKFGAKCRGITLSPVQAKRAQEISAAEGLSDKVC 180

Query: 181 FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGSTIIIVTWCHRNLGP 240
           FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRV APG TIIIVTWCHR+LGP
Sbjct: 181 FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVTAPGGTIIIVTWCHRDLGP 240

Query: 241 SENSLQPWEEKLLQKICDGFYLPAWCSTADYVKLLESHNLQDIKTADWSQNVAPFWPAVI 300
           SE+SLQPWE KLLQKICDGFYLPAWCSTADYVKLL+SHNLQDIK ADWS+NVAPFWPAVI
Sbjct: 241 SESSLQPWELKLLQKICDGFYLPAWCSTADYVKLLQSHNLQDIKRADWSENVAPFWPAVI 300

Query: 301 RSALTWKGFTSLLRSG 315
           RSA TWKGFTSLLR+G
Sbjct: 301 RSAFTWKGFTSLLRTG 311

[Close]

BLAST of Lsi10G002240 vs. NCBI nr
Match: gi|567889795|ref|XP_006437418.1| (hypothetical protein CICLE_v10032026mg [Citrus clementina])

HSP 1 Score: 434.5 bits (1116), Expect = 1.6e-118
Identity = 208/273 (76.19%), Postives = 233/273 (85.35%), Query Frame = 1


Query: 52  ASATTPADIQMDAD--RLQKGIAEFYDESSGLWESVWGEHMHHGFYDPDSSISLSDHRSA 111
           AS+TT      DA    L++GIAEFYDESS LWE +WG+HMHHGFY+PDSS+S+SDHR+A
Sbjct: 38  ASSTTSTTTMSDAAARELKEGIAEFYDESSSLWEDIWGDHMHHGFYEPDSSVSVSDHRAA 97

Query: 112 QIRMIEETLRFAGVEAATA-----AVDVGCGIGGSSRYLAKKFGAKCRGITLSPVQAKRA 171
           Q+RMIEETLRFAGV           VDVGCGIGGSSRYLAKKFGAKC+GITLSPVQA+RA
Sbjct: 98  QVRMIEETLRFAGVSEDPTKRPKNVVDVGCGIGGSSRYLAKKFGAKCQGITLSPVQAQRA 157

Query: 172 QAIAAAEGLSDKVCFEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGS 231
            A+AAA GL+DKV F+V DAL QPF DG+FDLVWSMESGEHMPDKSKFVSEL RV AP  
Sbjct: 158 NALAAARGLADKVSFQVGDALQQPFPDGQFDLVWSMESGEHMPDKSKFVSELARVTAPAG 217

Query: 232 TIIIVTWCHRNLGPSENSLQPWEEKLLQKICDGFYLPAWCSTADYVKLLESHNLQDIKTA 291
           TIIIVTWCHR+L PSE SLQPWE++LL+KICD +YLPAWCSTADYVKLL+S +L+DIK  
Sbjct: 218 TIIIVTWCHRDLAPSEESLQPWEQELLKKICDAYYLPAWCSTADYVKLLQSLSLEDIKAE 277

Query: 292 DWSQNVAPFWPAVIRSALTWKGFTSLLRSGKYS 318
           DWSQNVAPFWPAVIRSALTWKGFTSLLR+GK S
Sbjct: 278 DWSQNVAPFWPAVIRSALTWKGFTSLLRTGKLS 310

[Close]

BLAST of Lsi10G002240 vs. NCBI nr
Match: gi|568862431|ref|XP_006484687.1| (PREDICTED: probable tocopherol O-methyltransferase, chloroplastic isoform X1 [Citrus sinensis])

HSP 1 Score: 433.7 bits (1114), Expect = 2.7e-118
Identity = 207/270 (76.67%), Postives = 232/270 (85.93%), Query Frame = 1


Query: 52  ASATTPADIQMDAD--RLQKGIAEFYDESSGLWESVWGEHMHHGFYDPDSSISLSDHRSA 111
           AS+TT      DA    L++GIAEFYDESS LWE +WG+HMHHGFY+PDSS+S+SDHR+A
Sbjct: 38  ASSTTSTTTMSDAAARELKEGIAEFYDESSSLWEDIWGDHMHHGFYEPDSSVSVSDHRAA 97

Query: 112 QIRMIEETLRFAGVEAATA-----AVDVGCGIGGSSRYLAKKFGAKCRGITLSPVQAKRA 171
           Q+RMIEETLRFAGV  A        VDVGCGIGGSSRYLAKKFGAKC+GITLSPVQA+RA
Sbjct: 98  QVRMIEETLRFAGVSEAPTKRPKNVVDVGCGIGGSSRYLAKKFGAKCQGITLSPVQAQRA 157

Query: 172 QAIAAAEGLSDKVCFEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGS 231
            A+AAA GL+DKV F+V DAL QPF DG+FDLVWSMESGEHMPDKSKFVSEL RV AP  
Sbjct: 158 NALAAARGLADKVSFQVGDALQQPFPDGQFDLVWSMESGEHMPDKSKFVSELARVTAPAG 217

Query: 232 TIIIVTWCHRNLGPSENSLQPWEEKLLQKICDGFYLPAWCSTADYVKLLESHNLQDIKTA 291
           TIIIVTWCHR+L PSE SLQPWE++LL+KICD +YLPAWCSTADYVKLL+S +L+DIK  
Sbjct: 218 TIIIVTWCHRDLAPSEESLQPWEQELLKKICDAYYLPAWCSTADYVKLLQSLSLEDIKAE 277

Query: 292 DWSQNVAPFWPAVIRSALTWKGFTSLLRSG 315
           DWSQNVAPFWPAVIRSALTWKGFTSLLR+G
Sbjct: 278 DWSQNVAPFWPAVIRSALTWKGFTSLLRTG 307

[Close]

BLAST of Lsi10G002240 vs. NCBI nr
Match: gi|641828878|gb|KDO48014.1| (hypothetical protein CISIN_1g016921mg [Citrus sinensis])

HSP 1 Score: 432.6 bits (1111), Expect = 6.1e-118
Identity = 207/273 (75.82%), Postives = 232/273 (84.98%), Query Frame = 1


Query: 52  ASATTPADIQMDAD--RLQKGIAEFYDESSGLWESVWGEHMHHGFYDPDSSISLSDHRSA 111
           AS+TT      DA    L++GIAEFYDESS LWE +WG+HMHHGFY+PDSS+S+SDHR+A
Sbjct: 78  ASSTTSTTTMSDAAARELKEGIAEFYDESSSLWEDIWGDHMHHGFYEPDSSVSVSDHRAA 137

Query: 112 QIRMIEETLRFAGVEAATA-----AVDVGCGIGGSSRYLAKKFGAKCRGITLSPVQAKRA 171
           Q+RMIEETLRFAGV           VDVGCGIGGSSRYLAKKFGAKC+GITLSPVQA+RA
Sbjct: 138 QVRMIEETLRFAGVSEDPTKRPKNVVDVGCGIGGSSRYLAKKFGAKCQGITLSPVQAQRA 197

Query: 172 QAIAAAEGLSDKVCFEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGS 231
            A+AAA GL+DKV F+V DAL QPF DG+FDLVWSMESGEHMPDKSKFVSEL RV AP  
Sbjct: 198 NALAAARGLADKVSFQVGDALQQPFPDGQFDLVWSMESGEHMPDKSKFVSELARVTAPAG 257

Query: 232 TIIIVTWCHRNLGPSENSLQPWEEKLLQKICDGFYLPAWCSTADYVKLLESHNLQDIKTA 291
           TIIIVTWCHR+L PSE SLQPWE++LL+KICD +YLPAWCSTADYVKLL+S +L+DIK  
Sbjct: 258 TIIIVTWCHRDLAPSEESLQPWEQELLKKICDAYYLPAWCSTADYVKLLQSLSLEDIKAE 317

Query: 292 DWSQNVAPFWPAVIRSALTWKGFTSLLRSGKYS 318
           DWSQNVAPFWPAVI SALTWKGFTSLLR+GK S
Sbjct: 318 DWSQNVAPFWPAVIHSALTWKGFTSLLRTGKLS 350

The following BLAST results are available for this feature:

BLAST of Lsi10G002240 vs. Swiss-Prot


Match Name	E-value	Identity	Description
GTOMC_ARATH	5.0e-112	63.69	Tocopherol O-methyltransferase, chloroplastic OS=Arabidopsis thaliana GN=VTE4 PE...	[more]
GTOMC_ORYSJ	3.7e-107	68.84	Probable tocopherol O-methyltransferase, chloroplastic OS=Oryza sativa subsp. ja...	[more]
BQMT_SYNY3	2.2e-35	36.07	2-methyl-6-phytyl-1,4-hydroquinone methyltransferase OS=Synechocystis sp. (strai...	[more]
REBMT_NOCAE	5.1e-24	36.48	Demethylrebeccamycin-D-glucose O-methyltransferase OS=Lechevalieria aerocolonige...	[more]
DMRMT_AMYMS	2.8e-22	30.80	27-O-demethylrifamycin SV methyltransferase OS=Amycolatopsis mediterranei (strai...	[more]

BLAST of Lsi10G002240 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LTM1_CUCSA	4.1e-153	85.76	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G432780 PE=4 SV=1	[more]
V4SS85_9ROSI	1.1e-118	76.19	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10032026mg PE=4 SV=1	[more]
A0A067DYF3_CITSI	4.2e-118	75.82	Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g016921mg PE=4 SV=1	[more]
V4TBP0_9ROSI	9.4e-118	76.30	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10005360mg PE=4 SV=1	[more]
A0A067EAQ8_CITSI	3.6e-117	75.93	Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g016921mg PE=4 SV=1	[more]

BLAST of Lsi10G002240 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G64970.1	2.8e-113	63.69	gamma-tocopherol methyltransferase	[more]
AT1G20330.1	7.1e-16	27.50	sterol methyltransferase 2	[more]
AT1G76090.1	7.9e-15	26.67	sterol methyltransferase 3	[more]
AT4G33110.1	1.0e-06	27.49	S-adenosyl-L-methionine-dependent methyltransferases superfamily pro...	[more]

BLAST of Lsi10G002240 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659125635\|ref\|XP_008462787.1\|	2.7e-158	87.90	PREDICTED: tocopherol O-methyltransferase, chloroplastic [Cucumis melo]	[more]
gi\|778673784\|ref\|XP_004151846.2\|	5.8e-153	85.76	PREDICTED: tocopherol O-methyltransferase, chloroplastic [Cucumis sativus]	[more]
gi\|567889795\|ref\|XP_006437418.1\|	1.6e-118	76.19	hypothetical protein CICLE_v10032026mg [Citrus clementina]	[more]
gi\|568862431\|ref\|XP_006484687.1\|	2.7e-118	76.67	PREDICTED: probable tocopherol O-methyltransferase, chloroplastic isoform X1 [Ci...	[more]
gi\|641828878\|gb\|KDO48014.1\|	6.1e-118	75.82	hypothetical protein CISIN_1g016921mg [Citrus sinensis]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0032259	methylation
GO:0010189	vitamin E biosynthetic process
GO:0008152	metabolic process

Vocabulary: **Molecular Function**
Term	Definition
GO:0051741	2-methyl-6-phytyl-1,4-benzoquinone methyltransferase activity
GO:0008168	methyltransferase activity

Vocabulary: **INTERPRO**
Term	Definition
IPR025774	MTs_g-TMT
IPR013216	Methyltransf_11

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032259	methylation
biological_process	GO:0010189	vitamin E biosynthetic process
biological_process	GO:0008152	metabolic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0051741	2-methyl-6-phytyl-1,4-benzoquinone methyltransferase activity
molecular_function	GO:0050342	tocopherol O-methyltransferase activity
molecular_function	GO:0008168	methyltransferase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi10G002240.1	Lsi10G002240.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013216	Methyltransferase type 11	PFAM	PF08241	Methyltransf_11	coord: 130..228 score: 8.7
IPR025774	SAM-dependent methyltransferase gamma-tocopherol (gTMT)-type	PROFILE	PS51581	SAM_GTMT	coord: 52..317 score: 123
None	No IPR available	PANTHER	PTHR10108	METHYLTRANSFERASE	coord: 71..302 score: 1.3E
None	No IPR available	PANTHER	PTHR10108:SF708	TOCOPHEROL O-METHYLTRANSFERASE, CHLOROPLASTIC	coord: 71..302 score: 1.3E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi10G002240	Csa2G432780	Cucumber (Chinese Long) v2	culsiB094
Lsi10G002240	CSPI02G27410	Wild cucumber (PI 183967)	cpilsiB092
Lsi10G002240	Cucsa.017420	Cucumber (Gy14) v1	cgylsiB424
Lsi10G002240	CmaCh20G009430	Cucurbita maxima (Rimu)	cmalsiB507
Lsi10G002240	CmoCh20G009540	Cucurbita moschata (Rifu)	cmolsiB491
Lsi10G002240	Cla006807	Watermelon (97103) v1	lsiwmB068
Lsi10G002240	MELO3C024736	Melon (DHL92) v3.5.1	lsimeB053
Lsi10G002240	ClCG02G007700	Watermelon (Charleston Gray)	lsiwcgB055
Lsi10G002240	MELO3C024736.2	Melon (DHL92) v3.6.1	lsimedB054
Lsi10G002240	Cla97C02G034400	Watermelon (97103) v2	lsiwmbB054
Lsi10G002240	Bhi10G001235	Wax gourd	lsiwgoB069
Lsi10G002240	Cp4.1LG16g01620	Cucurbita pepo (Zucchini)	cpelsiB220
Lsi10G002240	CsGy2G026840	Cucumber (Gy14) v2	cgyblsiB087
Lsi10G002240	Carg25475	Silver-seed gourd	carlsiB870
Lsi10G002240	CsaV3_2G036010	Cucumber (Chinese Long) v3	cuclsiB105

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi10G002240	Silver-seed gourd	carlsiB961
Lsi10G002240	Cucurbita maxima (Rimu)	cmalsiB548
Lsi10G002240	Cucurbita moschata (Rifu)	cmolsiB533
Lsi10G002240	Cucurbita pepo (Zucchini)	cpelsiB589