Lsi10G002060 (gene) Bottle gourd (USVL1VR-Ls)

Overview
NameLsi10G002060
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionGDSL esterase/lipase
Locationchr10 : 3404158 .. 3405668 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAGGAGTTAATTGCCTCATTGTTGCTCTATCACTCCACATAATTTGGCTGCTATTTCTTAGCAAACCATGCAGTGCTTTAGAGCCTAAAACTTCTCGATCGTTTCCCGCCATTCTCATATTCGGTGATTCTACAGTGGATACCGGAAATAACAACTTCATTCACACCATTTTCAAGGGCAATTACTTTCCATATGGCAAAAATTTTCCTGGTCATGTTGCTACTGGAAGGTTCAGTGATGGAAAACTCATTCCCGACATGGTAGCTTCTAAACTAGGGATAAAAGAGCTTGTTCCTCCATTTTTGGATCCAAAACTATCAAATGATGACATAAAAACAGGGGTCAGTTTTGCATCGGCTGGCACGGGATTCGATGATCTAACTGCTGCTATATCCAAAGTCATTCCTGTGATGAAGCAAATTGATCTTTTCAAGAACTACATTGAAAGGCTTCAAAGGATTGTGGGTGTGGATGAAAGTAGGAGGATTATTGGCAGTGCTTTGGTTATTATTAGTGCAGGAACCAATGACTTGAACATCAATTTCTATGACCTTCCCACAAGGCAGTTGCATTACAATATTAGTGGCTATCAAGATTTTCTACAGAATAGGCTACAAAGCTTGATCAAGGTAAATTCTAAATATTTGACCTGAGAATCTCAGACCTGTTTGGATTGATTTTCTAAGTGTTTATAAACATTTTTTTTCACTTAAACACTTATTTAAACACTTAGAAAGTCAATCCAAACGCACCATCAATTTATCCCTGTACTTGAAGAGAATTTAGTAGGTCTTTAAACTATAGTCCTATTTTTAGAAAGTTACTTTAAAAATACTATAATAAGCCTCTAAATTCTAATCGTGTTACTATTAGTTTACACTTGAACCAACTGAAAATGAACACTTTAACAGGAAATTTACCAACTTGGATGCCGAAACATAGTGGTTGCTGGCCTCCCACCTATTGGTTGTCTTCCCATTCAAGAGACCATAGCATTTGAGAATCCTCTAAAGCGAAGTTGTTTGGAAGATCAAAACTCAGATTCCAAAGCCTACAATCAGAAGCTTTCAAAGCTGTTGAATAATTTACAGCCACAGCTCTCTGGAAGCAAAATTTTGTATGCAGACATTTACAACCCCCTCATCGACATGGTAAACAATCCTAAGAAATATGGTAAGTCAGTGCCCTGCCACTCAAAATTGAATAACCCCATCTACTTATTGAATCTAATGCAGTAAATATAAAAGAGAAATCAAGTTCTGAAAACCATTTTCACCTAAAAATTTTCATTGGGGCATTTCATCTAACAGGCTTTGAGCAGACGAACAGAGGTTGCTGTGGAACTGGATTAGCAGAAGCAGGGCCTTTGTGTAATCGAATAACCCCAACGTGTGAAAACTCTTCAAAATTTATGTTTTGGGATAGCATTCATCCGACTGAAGCAGCCTATAAATTCATCGCTGAGTCACTTCTCGAGAAACTTGGCGATCCCCTGAATTGGAATTGA

mRNA sequence

ATGGCAGGAGTTAATTGCCTCATTGTTGCTCTATCACTCCACATAATTTGGCTGCTATTTCTTAGCAAACCATGCAGTGCTTTAGAGCCTAAAACTTCTCGATCGTTTCCCGCCATTCTCATATTCGGTGATTCTACAGTGGATACCGGAAATAACAACTTCATTCACACCATTTTCAAGGGCAATTACTTTCCATATGGCAAAAATTTTCCTGGTCATGTTGCTACTGGAAGGTTCAGTGATGGAAAACTCATTCCCGACATGGTAGCTTCTAAACTAGGGATAAAAGAGCTTGTTCCTCCATTTTTGGATCCAAAACTATCAAATGATGACATAAAAACAGGGGTCAGTTTTGCATCGGCTGGCACGGGATTCGATGATCTAACTGCTGCTATATCCAAAGTCATTCCTGTGATGAAGCAAATTGATCTTTTCAAGAACTACATTGAAAGGCTTCAAAGGATTGTGGGTGTGGATGAAAGTAGGAGGATTATTGGCAGTGCTTTGGAAATTTACCAACTTGGATGCCGAAACATAGTGGTTGCTGGCCTCCCACCTATTGGTTGTCTTCCCATTCAAGAGACCATAGCATTTGAGAATCCTCTAAAGCGAAGTTGTTTGGAAGATCAAAACTCAGATTCCAAAGCCTACAATCAGAAGCTTTCAAAGCTGTTGAATAATTTACAGCCACAGCTCTCTGGAAGCAAAATTTTGTATGCAGACATTTACAACCCCCTCATCGACATGGTAAACAATCCTAAGAAATATGGCTTTGAGCAGACGAACAGAGGTTGCTGTGGAACTGGATTAGCAGAAGCAGGGCCTTTGTGTAATCGAATAACCCCAACGTGTGAAAACTCTTCAAAATTTATGTTTTGGGATAGCATTCATCCGACTGAAGCAGCCTATAAATTCATCGCTGAGTCACTTCTCGAGAAACTTGGCGATCCCCTGAATTGGAATTGA

Coding sequence (CDS)

ATGGCAGGAGTTAATTGCCTCATTGTTGCTCTATCACTCCACATAATTTGGCTGCTATTTCTTAGCAAACCATGCAGTGCTTTAGAGCCTAAAACTTCTCGATCGTTTCCCGCCATTCTCATATTCGGTGATTCTACAGTGGATACCGGAAATAACAACTTCATTCACACCATTTTCAAGGGCAATTACTTTCCATATGGCAAAAATTTTCCTGGTCATGTTGCTACTGGAAGGTTCAGTGATGGAAAACTCATTCCCGACATGGTAGCTTCTAAACTAGGGATAAAAGAGCTTGTTCCTCCATTTTTGGATCCAAAACTATCAAATGATGACATAAAAACAGGGGTCAGTTTTGCATCGGCTGGCACGGGATTCGATGATCTAACTGCTGCTATATCCAAAGTCATTCCTGTGATGAAGCAAATTGATCTTTTCAAGAACTACATTGAAAGGCTTCAAAGGATTGTGGGTGTGGATGAAAGTAGGAGGATTATTGGCAGTGCTTTGGAAATTTACCAACTTGGATGCCGAAACATAGTGGTTGCTGGCCTCCCACCTATTGGTTGTCTTCCCATTCAAGAGACCATAGCATTTGAGAATCCTCTAAAGCGAAGTTGTTTGGAAGATCAAAACTCAGATTCCAAAGCCTACAATCAGAAGCTTTCAAAGCTGTTGAATAATTTACAGCCACAGCTCTCTGGAAGCAAAATTTTGTATGCAGACATTTACAACCCCCTCATCGACATGGTAAACAATCCTAAGAAATATGGCTTTGAGCAGACGAACAGAGGTTGCTGTGGAACTGGATTAGCAGAAGCAGGGCCTTTGTGTAATCGAATAACCCCAACGTGTGAAAACTCTTCAAAATTTATGTTTTGGGATAGCATTCATCCGACTGAAGCAGCCTATAAATTCATCGCTGAGTCACTTCTCGAGAAACTTGGCGATCCCCTGAATTGGAATTGA

Protein sequence

MAGVNCLIVALSLHIIWLLFLSKPCSALEPKTSRSFPAILIFGDSTVDTGNNNFIHTIFKGNYFPYGKNFPGHVATGRFSDGKLIPDMVASKLGIKELVPPFLDPKLSNDDIKTGVSFASAGTGFDDLTAAISKVIPVMKQIDLFKNYIERLQRIVGVDESRRIIGSALEIYQLGCRNIVVAGLPPIGCLPIQETIAFENPLKRSCLEDQNSDSKAYNQKLSKLLNNLQPQLSGSKILYADIYNPLIDMVNNPKKYGFEQTNRGCCGTGLAEAGPLCNRITPTCENSSKFMFWDSIHPTEAAYKFIAESLLEKLGDPLNWN
Homology
[Close]
BLAST of Lsi10G002060 vs. Swiss-Prot
Match: GDL41_ARATH (GDSL esterase/lipase At2g30220 OS=Arabidopsis thaliana GN=At2g30220 PE=3 SV=1)

HSP 1 Score: 295.4 bits (755), Expect = 7.4e-79
Identity = 161/343 (46.94%), Postives = 207/343 (60.35%), Query Frame = 1 

Query: 11  LSLHIIWLLFLSK---PCSALEPKTSRSFPAILIFGDSTVDTGNNNFI-HTIFKGNYFPY 70
           +S  I++ LF++     C+A    T   FPAILIFGDST DTGNNN+    +FK N+ PY
Sbjct: 3   ISKTIVFGLFVATLLVSCNADANTTQPLFPAILIFGDSTADTGNNNYYSQAVFKANHLPY 62

Query: 71  GKNFPGHVATGRFSDGKLIPDMVASKLGIKELVPPFLDPKLSNDDIKTGVSFASAGTGFD 130
           G + PGH A GRFS+GKLI D++++KL IKE VPPFL P +S+ DI TGV FASAG G+D
Sbjct: 63  GVDLPGHEANGRFSNGKLISDVISTKLNIKEFVPPFLQPNISDQDIVTGVCFASAGAGYD 122

Query: 131 DLTAAISKVIPVMKQI-----------------------------------DLFKNY--- 190
           D T+  SK IPV +Q                                    D   N+   
Sbjct: 123 DETSLSSKAIPVSQQPSMFKNYIARLKGIVGDKKAMEIINNALVVISAGPNDFILNFYDI 182

Query: 191 -IERLQ--RIVGVDES--RRIIGSALEIYQLGCRNIVVAGLPPIGCLPIQETIAFENPLK 250
            I RL+   I G  +   +R+ G   E+Y LGCRNI+V GLPP+GCLPIQ T      L 
Sbjct: 183 PIRRLEYPTIYGYQDFVLKRLDGFVRELYSLGCRNILVGGLPPMGCLPIQLTAKLRTILG 242

Query: 251 RSCLEDQNSDSKAYNQKLSKLLNNLQPQLSGSKILYADIYNPLIDMVNNPKKYGFEQTNR 307
             C+E +N DS  YNQKL K L  +Q  L GSK LYA++Y+P++DM+ NP KYGF++T +
Sbjct: 243 -ICVEQENKDSILYNQKLVKKLPEIQASLPGSKFLYANVYDPVMDMIRNPSKYGFKETKK 302

[Close]
BLAST of Lsi10G002060 vs. Swiss-Prot
Match: GDL43_ARATH (GDSL esterase/lipase At2g31540 OS=Arabidopsis thaliana GN=At2g31540 PE=2 SV=1)

HSP 1 Score: 283.5 bits (724), Expect = 2.9e-75
Identity = 157/354 (44.35%), Postives = 205/354 (57.91%), Query Frame = 1 

Query: 10  ALSLHIIWLLFLSKPCSALEPKTSRS-FPAILIFGDSTVDTGNNNF-IHTIFKGNYFPYG 69
           A++L +     L  PC+A    T++  FPAILIFGDSTVDTGNNN+ + TIF+  +FPYG
Sbjct: 6   AITLTLFIATTLLAPCNAAANATTKPLFPAILIFGDSTVDTGNNNYPLPTIFRAEHFPYG 65

Query: 70  KNFPGHVATGRFSDGKLIPDMVASKLGIKELVPPFLDPKLSNDDIKTGVSFASAGTG--- 129
            + P   A GRFS+GKLI D++A+KL IKE +PPFL P LS+ DI TGV FASAG G   
Sbjct: 66  MDLPDGKANGRFSNGKLISDIIATKLNIKEFIPPFLQPNLSDQDILTGVCFASAGAGYDD 125

Query: 130 -----------------FDDLTAAISKVIPVMKQIDLFKN---------------YIERL 189
                            F    A +  ++   K +++  N               Y E  
Sbjct: 126 LTSLSTQAIRVSEQPNMFKSYIARLKGIVGDKKAMEIINNAFVVVSAGPNDFILNYYEIP 185

Query: 190 QR------IVGVDES--RRIIGSALEIYQLGCRNIVVAGLPPIGCLPIQETIAFENPLKR 249
            R      I G  +   +R+     E+Y LG RN++V GLPP+GCLPI  T  F N + R
Sbjct: 186 SRRLEYPFISGYQDFILKRLENFVRELYSLGVRNVLVGGLPPMGCLPIHMTAKFRN-IFR 245

Query: 250 SCLEDQNSDSKAYNQKLSKLLNNLQPQLSGSKILYADIYNPLIDMVNNPKKYGFEQTNRG 309
            CLE  N DS  YN+KL  LL  ++  L GSK LYAD+YNP+++M+ NP KYGF++T RG
Sbjct: 246 FCLEHHNKDSVLYNEKLQNLLPQIEASLPGSKFLYADVYNPMMEMIQNPSKYGFKETKRG 305

Query: 310 CCGTGLAEAGPLCNRITPTCENSSKFMFWDSIHPTEAAYKFIAESLLEKLGDPL 319
           CCGTG  E   +CN  +P C+N S+F+F+DSIHP+EA Y  I   L     DPL
Sbjct: 306 CCGTGFLETSFMCNVFSPVCQNRSEFLFFDSIHPSEATYNVIGNLL-----DPL 353

[Close]
BLAST of Lsi10G002060 vs. Swiss-Prot
Match: GDL44_ARATH (GDSL esterase/lipase At2g31550 OS=Arabidopsis thaliana GN=At2g31550 PE=3 SV=3)

HSP 1 Score: 282.3 bits (721), Expect = 6.4e-75
Identity = 159/355 (44.79%), Postives = 205/355 (57.75%), Query Frame = 1 

Query: 9   VALSLHIIWLLFLSKPCSALEPKTSRS-FPAILIFGDSTVDTGNNNF-IHTIFKGNYFPY 68
           + L+L I   L  S  C A    T++  FPAILIFGDSTVDTGNNN+ + TIF+  +FPY
Sbjct: 7   ITLTLFITTTLLAS--CDAAANATTKPLFPAILIFGDSTVDTGNNNYPLPTIFRAEHFPY 66

Query: 69  GKNFPGHVATGRFSDGKLIPDMVASKLGIKELVPPFLDPKLSNDDIKTGVSFASAGTG-- 128
           G + P   A GRFS+GKLI D++A+KL IKE +PPFL P LS+ DI TGV FASAG G  
Sbjct: 67  GMDLPDGKANGRFSNGKLISDIIATKLNIKEFIPPFLQPNLSDQDILTGVCFASAGAGYD 126

Query: 129 ------------------FDDLTAAISKVIPVMKQIDLFKNYI----------------- 188
                             F    A +  ++   K +++  N                   
Sbjct: 127 DLTSLSTQAIRVSEQPNMFKSYIARLKGIVGDKKAMEIINNAFVVVSAGPNDFILNYYDI 186

Query: 189 --ERLQR--IVGVDES--RRIIGSALEIYQLGCRNIVVAGLPPIGCLPIQETIAFENPLK 248
              RL+   I G  +   +R+     E+Y LG RN++V GLPP+GCLPI  T  F N + 
Sbjct: 187 PSRRLEYPFISGYQDFILKRLENFVRELYSLGVRNVLVGGLPPMGCLPIHMTAKFRN-IF 246

Query: 249 RSCLEDQNSDSKAYNQKLSKLLNNLQPQLSGSKILYADIYNPLIDMVNNPKKYGFEQTNR 308
           R CLE  N DS  YN+KL KLL  ++  L GSK LYAD+YNP+++M+ NP KYGF++T R
Sbjct: 247 RFCLEHHNKDSVLYNEKLQKLLPQIEASLPGSKFLYADVYNPMMEMIQNPSKYGFKETKR 306

Query: 309 GCCGTGLAEAGPLCNRITPTCENSSKFMFWDSIHPTEAAYKFIAESLLEKLGDPL 319
           GCCGTG  E   +CN  +P C+N S+FMF+DSIHP+EA Y  I   L     DPL
Sbjct: 307 GCCGTGFLETSFMCNVFSPVCQNRSEFMFFDSIHPSEATYNVIGNRL-----DPL 353

[Close]
BLAST of Lsi10G002060 vs. Swiss-Prot
Match: GDL1_ARATH (GDSL esterase/lipase At1g06990 OS=Arabidopsis thaliana GN=At1g06990 PE=2 SV=2)

HSP 1 Score: 259.6 bits (662), Expect = 4.5e-68
Identity = 135/323 (41.80%), Postives = 189/323 (58.51%), Query Frame = 1 

Query: 36  FPAILIFGDSTVDTGNNNFIHTIFKGNYFPYGKNFPGHVATGRFSDGKLIPDMVASKLGI 95
           FPAIL+FGDST+DTGNNN+I T  + N+ PYG NFPGH ATGRFS+GKLIPD +AS +GI
Sbjct: 35  FPAILVFGDSTIDTGNNNYIKTYIRANFPPYGCNFPGHNATGRFSNGKLIPDFIASLMGI 94

Query: 96  KELVPPF---------------------------------LDPKLSNDDIKTGVSFASAG 155
           K+ VPPF                                 L      D +++ V   S  
Sbjct: 95  KDTVPPFLDPHLSDSDIITGVCFASAGSGYDNLTDRATSTLSVDKQADMLRSYVERLSQI 154

Query: 156 TGFDDLTAAISKVIPVMKQ------IDLFKNYIERLQRIVGVDESRRIIGSAL-----EI 215
            G +   + +S+ + ++        ++L+     R +  +GVD  +  I S +     E+
Sbjct: 155 VGDEKAASIVSEALVIVSSGTNDFNLNLYDTPSRRQK--LGVDGYQSFILSNVHNFVQEL 214

Query: 216 YQLGCRNIVVAGLPPIGCLPIQETIAFENPLKRSCLEDQNSDSKAYNQKLSKLLNNLQPQ 275
           Y +GCR I+V GLPP+GCLPIQ T+A +   +R C++ QNSDS+ +NQKL   L  +Q  
Sbjct: 215 YDIGCRKIMVLGLPPVGCLPIQMTMAMQKQNERRCIDKQNSDSQEFNQKLKNSLTEMQSN 274

Query: 276 LSGSKILYADIYNPLIDMVNNPKKYGFEQTNRGCCGTGLAEAGPLCNRITPTCENSSKFM 315
           L+GS I Y DIY  L DM  NP++YG ++T RGCCGTG  E   LCN +T  C N ++++
Sbjct: 275 LTGSVIFYGDIYGALFDMATNPQRYGLKETTRGCCGTGEIELAYLCNALTRICPNPNQYL 334

[Close]
BLAST of Lsi10G002060 vs. Swiss-Prot
Match: APG2_ARATH (GDSL esterase/lipase APG OS=Arabidopsis thaliana GN=APG PE=2 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 3.2e-58
Identity = 122/338 (36.09%), Postives = 185/338 (54.73%), Query Frame = 1 

Query: 18  LLFLSKPCSALEPKTSRSFPAILIFGDSTVDTGNNNFIHTIFKGNYFPYGKNFPGHVATG 77
           LL L    S L+   ++  PAI+ FGDS VD GNNN++ T+F+ +Y PYG++F  H ATG
Sbjct: 10  LLTLVSTLSILQISFAQLVPAIMTFGDSVVDVGNNNYLPTLFRADYPPYGRDFANHKATG 69

Query: 78  RFSDGKLIPDMVASKLGIKELVPPFLDPKLSNDDIKTGVSFASAGTGFDDLTAAISKVIP 137
           RF +GKL  D+ A  LG  +  P +L P+ S  ++  G +FASA +G+DD  A ++  IP
Sbjct: 70  RFCNGKLATDITAETLGFTKYPPAYLSPEASGKNLLIGANFASAASGYDDKAALLNHAIP 129

Query: 138 VMKQIDLFKNYIERLQRIVGVDESRRIIGSALEIYQLGCRNIV----------------- 197
           + +Q++ FK Y  +L +I G  ++  II  A+ +   G  + V                 
Sbjct: 130 LYQQVEYFKEYKSKLIKIAGSKKADSIIKGAICLLSAGSSDFVQNYYVNPLLYKVYTVDA 189

Query: 198 -------------------------VAGLPPIGCLPIQETIAFENPLKRSCLEDQNSDSK 257
                                    V  LPP GCLP   T+   +  ++ C+   N+D++
Sbjct: 190 YGSFLIDNFSTFIKQVYAVGARKIGVTSLPPTGCLPAARTLFGFH--EKGCVSRLNTDAQ 249

Query: 258 AYNQKLSKLLNNLQPQLSGSKILYADIYNPLIDMVNNPKKYGFEQTNRGCCGTGLAE-AG 312
            +N+KL+   + LQ Q S  KI+  DIY+PL D+V NP K GF +  +GCCGTG  E   
Sbjct: 250 NFNKKLNAAASKLQKQYSDLKIVVFDIYSPLYDLVQNPSKSGFTEATKGCCGTGTVETTS 309

[Close]
BLAST of Lsi10G002060 vs. TrEMBL
Match: A0A0A0LNE2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G431080 PE=4 SV=1)

HSP 1 Score: 433.0 bits (1112), Expect = 3.3e-118
Identity = 229/364 (62.91%), Postives = 255/364 (70.05%), Query Frame = 1 

Query: 1   MAGVNCLIVALSLHIIWLLF-LSKPCSALEPKTSRSFPAILIFGDSTVDTGNNNFIHTIF 60
           MA VNCLI ALSLH IWLL  L+KPCS+LEPKT+ SFPAILIFGDSTVDTGNNNFI TIF
Sbjct: 1   MARVNCLIAALSLHTIWLLVVLTKPCSSLEPKTTPSFPAILIFGDSTVDTGNNNFIPTIF 60

Query: 61  KGNYFPYGKNFPGHVATGRFSDGKLIPDMVASKLGI------------------------ 120
           KGNY PYGKNFPGH+ATGRFSDGKLIPDMVAS+LGI                        
Sbjct: 61  KGNYSPYGKNFPGHLATGRFSDGKLIPDMVASRLGIKELVPPFLDPKLSNDDIKTGVSFA 120

Query: 121 ---------KELVPPFLDPKLSNDDIKTGVSFASAGTGFDDLTAAISKVIPVMK------ 180
                       +   +      D  K  +       G D+    I+  + V+       
Sbjct: 121 SAGTGFDDLTAAISKVIPVMKQIDHFKNYIQRLQGVVGVDESKRIINNALVVISAGTNDL 180

Query: 181 QIDLFKNYIERLQ-RIVGVDE--SRRIIGSALEIYQLGCRNIVVAGLPPIGCLPIQETIA 240
            I+ +     +LQ  I G  +    R+     EIYQLGCRNIVVAGLPP+GCLPIQETIA
Sbjct: 181 NINFYDLPTRQLQYNISGYQDFLQNRLQSLIKEIYQLGCRNIVVAGLPPVGCLPIQETIA 240

Query: 241 FENPLKRSCLEDQNSDSKAYNQKLSKLLNNLQPQLSGSKILYADIYNPLIDMVNNPKKYG 300
           FENPLKR+CL+DQNSDS AYNQKLSKLL NLQPQL+GSKILYADIY PLIDM+NNP+KYG
Sbjct: 241 FENPLKRNCLKDQNSDSVAYNQKLSKLLTNLQPQLAGSKILYADIYTPLIDMLNNPQKYG 300

Query: 301 FEQTNRGCCGTGLAEAGPLCNRITPTCENSSKFMFWDSIHPTEAAYKFIAESLLEKLGDP 322
           F+ TNRGCCGTGL EAGPLCN  TPTCENSSKFMFWDSIHPTEAAYKFIAE+LL+KLGD 
Sbjct: 301 FDHTNRGCCGTGLVEAGPLCNPKTPTCENSSKFMFWDSIHPTEAAYKFIAEALLKKLGDT 360

[Close]
BLAST of Lsi10G002060 vs. TrEMBL
Match: A0A0A0KF79_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G180980 PE=4 SV=1)

HSP 1 Score: 379.4 bits (973), Expect = 4.3e-102
Identity = 208/361 (57.62%), Postives = 243/361 (67.31%), Query Frame = 1 

Query: 1   MAGVNCLIVALSLHIIWLLFLSKPCSALEPKTSRSFPAILIFGDSTVDTGNNNFIHTIFK 60
           MA  N LI ALS+HIIWLLFLSKPCSAL PKTSRSF ++LIFGDSTVDTGNNNFI TIFK
Sbjct: 1   MARANYLIAALSIHIIWLLFLSKPCSALAPKTSRSFSSVLIFGDSTVDTGNNNFIPTIFK 60

Query: 61  GNYFPYGKNFPGHVATGRFSDGKLI-------------------PDM-----------VA 120
            NY+PYGK+FPGHVATGRFSDGKLI                   P++            +
Sbjct: 61  ANYWPYGKDFPGHVATGRFSDGKLIPDMVASKLGIKELVPPFLDPELSDDDVKTGVSFAS 120

Query: 121 SKLGIKELVPPF--LDPKLSNDDI-KTGVSFASAGTGFDDLTAAISKVIPVMK--QIDLF 180
           +  G+ +L      + P +   D+ K  +       G D+    I   + V+     DL 
Sbjct: 121 AGTGVDDLTAAISKVIPAMKQIDMFKNYIQRLQRIVGVDESKRIIGSALAVISVGTNDLT 180

Query: 181 KNYIE----RLQ-RIVGVDE--SRRIIGSALEIYQLGCRNIVVAGLPPIGCLPIQETIAF 240
            N+ +    +LQ  I G  E    R+     EIYQLGCR IVVAGLPPIGCLPIQETI+ 
Sbjct: 181 FNFYDIPTRQLQYNISGYQEFLQNRLQSLIKEIYQLGCRTIVVAGLPPIGCLPIQETISS 240

Query: 241 ENPLKRSCLEDQNSDSKAYNQKLSKLLNNLQPQLSGSKILYADIYNPLIDMVNNPKKYGF 300
             PL R CLE QN D++AYNQKLSKLL +LQPQL GS+ILYADIY PL+DM+NNP+KYGF
Sbjct: 241 PIPLNRRCLEYQNKDAEAYNQKLSKLLGSLQPQLPGSQILYADIYTPLMDMINNPQKYGF 300

Query: 301 EQTNRGCCGTGLAEAGPLCNRITPTCENSSKFMFWDSIHPTEAAYKFIAESLLEKLGDPL 320
           EQTN GCCGTGL EAGPLCN+ITPTCE+ SKFMFWDSIHP+EA YKF+ ESLL +  D L
Sbjct: 301 EQTNIGCCGTGLVEAGPLCNKITPTCEDPSKFMFWDSIHPSEATYKFVTESLLNQFFDRL 360

[Close]
BLAST of Lsi10G002060 vs. TrEMBL
Match: A0A103XNX7_CYNCS (Lipase, GDSL OS=Cynara cardunculus var. scolymus GN=Ccrd_003829 PE=4 SV=1)

HSP 1 Score: 376.7 bits (966), Expect = 2.8e-101
Identity = 169/306 (55.23%), Postives = 238/306 (77.78%), Query Frame = 1 

Query: 13  LHIIWLLFLSKPCSALEPKTSRSFPAILIFGDSTVDTGNNNFIHTIFKGNYFPYGKNFPG 72
           LH+ + L L +         +  FPAIL+FGDSTVDTGNNN+I T+ K N++PYGK+FPG
Sbjct: 2   LHLPFFLLLLQVLPQKTNGQNFKFPAILVFGDSTVDTGNNNYITTLSKANHYPYGKDFPG 61

Query: 73  HVATGRFSDGKLIPDMVASKLGIKELVPPFLDPKLSNDDIKTGVSFASAGTGFDDLTAAI 132
           HV TGRFS+GKL  D++AS LG+K+ +PPFL P LS  +++TGV FASAGTG+D LTAA+
Sbjct: 62  HVPTGRFSNGKLAVDLLASLLGVKQTIPPFLQPDLSAYELQTGVCFASAGTGYDRLTAAL 121

Query: 133 SKVIPVMKQIDLFKNYIERLQRIVGVDESRRIIGSALEIYQLGCRNIVVAGLPPIGCLPI 192
           ++VIP+ +Q++ FK+Y ERL+++ G + ++RIIG ALE+Y LGCR +++ GLPPIGCLPI
Sbjct: 122 TRVIPMSQQLNYFKSYRERLKKMAGEEAAQRIIGQALELYNLGCRTLIITGLPPIGCLPI 181

Query: 193 QETIAFENPLKRSCLEDQNSDSKAYNQKLSKLLNNLQPQLSGSKILYADIYNPLIDMVNN 252
           Q T  F+    R+C+E+QN D +AYN+KL +LL ++Q  L GSKI YADIY P++DM+ +
Sbjct: 182 QITAKFKGQFGRTCVEEQNVDGQAYNRKLIELLPHIQSSLEGSKIFYADIYKPIMDMIKS 241

Query: 253 PKKYGFEQTNRGCCGTGLAEAGPLCNRITPTCENSSKFMFWDSIHPTEAAYKFIAESLLE 312
           P+K+GF +T +GCCGTGL EAGP+C  +TP C+NSS+++F+DSIHP+E AY+++ E L +
Sbjct: 242 PEKHGFRETMKGCCGTGLLEAGPICTPLTPLCQNSSEYLFFDSIHPSEKAYRYVTECLKK 301

Query: 313 KLGDPL 319
           ++ D L
Sbjct: 302 QILDKL 307

[Close]
BLAST of Lsi10G002060 vs. TrEMBL
Match: M1DB51_SOLTU (Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400035739 PE=4 SV=1)

HSP 1 Score: 365.5 bits (937), Expect = 6.4e-98
Identity = 166/298 (55.70%), Postives = 229/298 (76.85%), Query Frame = 1 

Query: 19  LFLSKPCSALEPKTSRSFPAILIFGDSTVDTGNNNFIHTIFKGNYFPYGKNFPGHVATGR 78
           + L + C  +  K+   FPAILIFGDSTVDTGNNN+I TIF+GN+ PYG+NFPG + TGR
Sbjct: 11  ILLVQHCIIINAKSVPKFPAILIFGDSTVDTGNNNYISTIFQGNHRPYGENFPGRIPTGR 70

Query: 79  FSDGKLIPDMVASKLGIKELVPPFLDPKLSNDDIKTGVSFASAGTGFDDLTAAISKVIPV 138
           FSDGKL+PD +AS LGIKE +PPFL P LSN D+ TGV+FASAG+G+DDLT   +KVIP+
Sbjct: 71  FSDGKLVPDFLASMLGIKEYIPPFLQPNLSNHDLLTGVNFASAGSGYDDLTTTATKVIPM 130

Query: 139 MKQIDLFKNYIERLQRIVGVDESRRIIGSAL--EIYQLGCRNIVVAGLPPIGCLPIQETI 198
             QI  F+ YI++LQ I+G ++++++I  AL  ++Y LG RN++VAGLPP+GCLPIQ  I
Sbjct: 131 SDQIKYFEQYIQKLQLIIGEEKAQKLISGALVVDLYNLGVRNMLVAGLPPVGCLPIQ--I 190

Query: 199 AFENPLKRSCLEDQNSDSKAYNQKLSKLLNNLQPQLSGSKILYADIYNPLIDMVNNPKKY 258
             ++P  R C++++N D+++YN KL+ LL  +Q  L GS ILY+D Y+P + M+N+PKKY
Sbjct: 191 TAKSPFLRKCIKEENFDAQSYNVKLATLLKQIQDALLGSNILYSDSYHPFMHMINHPKKY 250

Query: 259 GFEQTNRGCCGTGLAEAGPLCNRITPTCENSSKFMFWDSIHPTEAAYKFIAESLLEKL 315
           GF +T RGCCG+G  EAGP CN+  P C+N+S+++FWDSIHP E+AY+ ++   ++KL
Sbjct: 251 GFLKTRRGCCGSGTYEAGPFCNKHRPVCKNASEYLFWDSIHPGESAYQHLSNMAMKKL 306

[Close]
BLAST of Lsi10G002060 vs. TrEMBL
Match: A0A0A0KC28_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G180990 PE=4 SV=1)

HSP 1 Score: 338.6 bits (867), Expect = 8.4e-90
Identity = 184/333 (55.26%), Postives = 220/333 (66.07%), Query Frame = 1 

Query: 1   MAGVNCLIVALSLHIIWLLFLSKPCSALEPKTSRSFPAILIFGDSTVDTGNNNFIHTIFK 60
           MA     I +LSLH+IWLLFLSKPC+ALEPK +RSF +ILIFGDSTVDTGNNNFI TIFK
Sbjct: 1   MAKATYHIASLSLHMIWLLFLSKPCTALEPKITRSFLSILIFGDSTVDTGNNNFISTIFK 60

Query: 61  GNYFPYGKNFPGHVAT-GRFSDGKLIPDMVASKLGIKEL---VPPFLDPKLSNDDIKTGV 120
            NY PYG +FPGH+   G+  + ++     ++  G  EL   V   +      D  K   
Sbjct: 61  ANYSPYGTDFPGHILNCGQRCENRV--GFASAGSGFDELTASVSNVISVMKQIDMFKNYT 120

Query: 121 SFASAGTGFDDLTAAISKVIPVMK------QIDLFKNYIERLQ-RIVGVDE--SRRIIGS 180
                  G D+    ++  + V+        I+ +   I +LQ  I G  +    R+   
Sbjct: 121 RRLQGIVGVDESRKILNSALVVISAGTNDVNINFYDLPIRQLQYNISGYQDFVQNRLQSL 180

Query: 181 ALEIYQLGCRNIVVAGLPPIGCLPIQETIAFENPLKRSCLEDQNSDSKAYNQKLSKLLNN 240
             EIYQLGCR IVVAGLPP+GCLPIQE+IAF+ P  R CLE+QNSD KAYNQKL+ LL+N
Sbjct: 181 IKEIYQLGCRTIVVAGLPPVGCLPIQESIAFQKPQDRKCLEEQNSDFKAYNQKLAHLLSN 240

Query: 241 LQPQLSGSKILYADIYNPLIDMVNNPKKYGFEQTNRGCCGTGLAEAGPLCN-RITPTCEN 300
           LQPQL GS ILY DIY PLIDMVNNP  YGFE  N GCCGTG+AEAGPLCN + +  CEN
Sbjct: 241 LQPQLPGSTILYGDIYTPLIDMVNNPHNYGFEHVNVGCCGTGMAEAGPLCNSKTSAICEN 300

Query: 301 SSKFMFWDSIHPTEAAYKFIAESLLEKLGDPLN 320
            SKFMFWDS+HP EAAY FI ESLL++  D LN
Sbjct: 301 PSKFMFWDSVHPIEAAYNFITESLLKQFVDRLN 331

[Close]
BLAST of Lsi10G002060 vs. TAIR10
Match: AT2G30220.1 (AT2G30220.1 GDSL-like Lipase/Acylhydrolase family protein)

HSP 1 Score: 295.4 bits (755), Expect = 4.1e-80
Identity = 161/343 (46.94%), Postives = 207/343 (60.35%), Query Frame = 1 

Query: 11  LSLHIIWLLFLSK---PCSALEPKTSRSFPAILIFGDSTVDTGNNNFI-HTIFKGNYFPY 70
           +S  I++ LF++     C+A    T   FPAILIFGDST DTGNNN+    +FK N+ PY
Sbjct: 3   ISKTIVFGLFVATLLVSCNADANTTQPLFPAILIFGDSTADTGNNNYYSQAVFKANHLPY 62

Query: 71  GKNFPGHVATGRFSDGKLIPDMVASKLGIKELVPPFLDPKLSNDDIKTGVSFASAGTGFD 130
           G + PGH A GRFS+GKLI D++++KL IKE VPPFL P +S+ DI TGV FASAG G+D
Sbjct: 63  GVDLPGHEANGRFSNGKLISDVISTKLNIKEFVPPFLQPNISDQDIVTGVCFASAGAGYD 122

Query: 131 DLTAAISKVIPVMKQI-----------------------------------DLFKNY--- 190
           D T+  SK IPV +Q                                    D   N+   
Sbjct: 123 DETSLSSKAIPVSQQPSMFKNYIARLKGIVGDKKAMEIINNALVVISAGPNDFILNFYDI 182

Query: 191 -IERLQ--RIVGVDES--RRIIGSALEIYQLGCRNIVVAGLPPIGCLPIQETIAFENPLK 250
            I RL+   I G  +   +R+ G   E+Y LGCRNI+V GLPP+GCLPIQ T      L 
Sbjct: 183 PIRRLEYPTIYGYQDFVLKRLDGFVRELYSLGCRNILVGGLPPMGCLPIQLTAKLRTILG 242

Query: 251 RSCLEDQNSDSKAYNQKLSKLLNNLQPQLSGSKILYADIYNPLIDMVNNPKKYGFEQTNR 307
             C+E +N DS  YNQKL K L  +Q  L GSK LYA++Y+P++DM+ NP KYGF++T +
Sbjct: 243 -ICVEQENKDSILYNQKLVKKLPEIQASLPGSKFLYANVYDPVMDMIRNPSKYGFKETKK 302

[Close]
BLAST of Lsi10G002060 vs. TAIR10
Match: AT2G31540.1 (AT2G31540.1 GDSL-like Lipase/Acylhydrolase superfamily protein)

HSP 1 Score: 283.5 bits (724), Expect = 1.6e-76
Identity = 157/354 (44.35%), Postives = 205/354 (57.91%), Query Frame = 1 

Query: 10  ALSLHIIWLLFLSKPCSALEPKTSRS-FPAILIFGDSTVDTGNNNF-IHTIFKGNYFPYG 69
           A++L +     L  PC+A    T++  FPAILIFGDSTVDTGNNN+ + TIF+  +FPYG
Sbjct: 6   AITLTLFIATTLLAPCNAAANATTKPLFPAILIFGDSTVDTGNNNYPLPTIFRAEHFPYG 65

Query: 70  KNFPGHVATGRFSDGKLIPDMVASKLGIKELVPPFLDPKLSNDDIKTGVSFASAGTG--- 129
            + P   A GRFS+GKLI D++A+KL IKE +PPFL P LS+ DI TGV FASAG G   
Sbjct: 66  MDLPDGKANGRFSNGKLISDIIATKLNIKEFIPPFLQPNLSDQDILTGVCFASAGAGYDD 125

Query: 130 -----------------FDDLTAAISKVIPVMKQIDLFKN---------------YIERL 189
                            F    A +  ++   K +++  N               Y E  
Sbjct: 126 LTSLSTQAIRVSEQPNMFKSYIARLKGIVGDKKAMEIINNAFVVVSAGPNDFILNYYEIP 185

Query: 190 QR------IVGVDES--RRIIGSALEIYQLGCRNIVVAGLPPIGCLPIQETIAFENPLKR 249
            R      I G  +   +R+     E+Y LG RN++V GLPP+GCLPI  T  F N + R
Sbjct: 186 SRRLEYPFISGYQDFILKRLENFVRELYSLGVRNVLVGGLPPMGCLPIHMTAKFRN-IFR 245

Query: 250 SCLEDQNSDSKAYNQKLSKLLNNLQPQLSGSKILYADIYNPLIDMVNNPKKYGFEQTNRG 309
            CLE  N DS  YN+KL  LL  ++  L GSK LYAD+YNP+++M+ NP KYGF++T RG
Sbjct: 246 FCLEHHNKDSVLYNEKLQNLLPQIEASLPGSKFLYADVYNPMMEMIQNPSKYGFKETKRG 305

Query: 310 CCGTGLAEAGPLCNRITPTCENSSKFMFWDSIHPTEAAYKFIAESLLEKLGDPL 319
           CCGTG  E   +CN  +P C+N S+F+F+DSIHP+EA Y  I   L     DPL
Sbjct: 306 CCGTGFLETSFMCNVFSPVCQNRSEFLFFDSIHPSEATYNVIGNLL-----DPL 353

[Close]
BLAST of Lsi10G002060 vs. TAIR10
Match: AT1G06990.1 (AT1G06990.1 GDSL-like Lipase/Acylhydrolase superfamily protein)

HSP 1 Score: 259.6 bits (662), Expect = 2.5e-69
Identity = 135/323 (41.80%), Postives = 189/323 (58.51%), Query Frame = 1 

Query: 36  FPAILIFGDSTVDTGNNNFIHTIFKGNYFPYGKNFPGHVATGRFSDGKLIPDMVASKLGI 95
           FPAIL+FGDST+DTGNNN+I T  + N+ PYG NFPGH ATGRFS+GKLIPD +AS +GI
Sbjct: 35  FPAILVFGDSTIDTGNNNYIKTYIRANFPPYGCNFPGHNATGRFSNGKLIPDFIASLMGI 94

Query: 96  KELVPPF---------------------------------LDPKLSNDDIKTGVSFASAG 155
           K+ VPPF                                 L      D +++ V   S  
Sbjct: 95  KDTVPPFLDPHLSDSDIITGVCFASAGSGYDNLTDRATSTLSVDKQADMLRSYVERLSQI 154

Query: 156 TGFDDLTAAISKVIPVMKQ------IDLFKNYIERLQRIVGVDESRRIIGSAL-----EI 215
            G +   + +S+ + ++        ++L+     R +  +GVD  +  I S +     E+
Sbjct: 155 VGDEKAASIVSEALVIVSSGTNDFNLNLYDTPSRRQK--LGVDGYQSFILSNVHNFVQEL 214

Query: 216 YQLGCRNIVVAGLPPIGCLPIQETIAFENPLKRSCLEDQNSDSKAYNQKLSKLLNNLQPQ 275
           Y +GCR I+V GLPP+GCLPIQ T+A +   +R C++ QNSDS+ +NQKL   L  +Q  
Sbjct: 215 YDIGCRKIMVLGLPPVGCLPIQMTMAMQKQNERRCIDKQNSDSQEFNQKLKNSLTEMQSN 274

Query: 276 LSGSKILYADIYNPLIDMVNNPKKYGFEQTNRGCCGTGLAEAGPLCNRITPTCENSSKFM 315
           L+GS I Y DIY  L DM  NP++YG ++T RGCCGTG  E   LCN +T  C N ++++
Sbjct: 275 LTGSVIFYGDIYGALFDMATNPQRYGLKETTRGCCGTGEIELAYLCNALTRICPNPNQYL 334

[Close]
BLAST of Lsi10G002060 vs. TAIR10
Match: AT3G16370.1 (AT3G16370.1 GDSL-like Lipase/Acylhydrolase superfamily protein)

HSP 1 Score: 226.9 bits (577), Expect = 1.8e-59
Identity = 122/338 (36.09%), Postives = 185/338 (54.73%), Query Frame = 1 

Query: 18  LLFLSKPCSALEPKTSRSFPAILIFGDSTVDTGNNNFIHTIFKGNYFPYGKNFPGHVATG 77
           LL L    S L+   ++  PAI+ FGDS VD GNNN++ T+F+ +Y PYG++F  H ATG
Sbjct: 10  LLTLVSTLSILQISFAQLVPAIMTFGDSVVDVGNNNYLPTLFRADYPPYGRDFANHKATG 69

Query: 78  RFSDGKLIPDMVASKLGIKELVPPFLDPKLSNDDIKTGVSFASAGTGFDDLTAAISKVIP 137
           RF +GKL  D+ A  LG  +  P +L P+ S  ++  G +FASA +G+DD  A ++  IP
Sbjct: 70  RFCNGKLATDITAETLGFTKYPPAYLSPEASGKNLLIGANFASAASGYDDKAALLNHAIP 129

Query: 138 VMKQIDLFKNYIERLQRIVGVDESRRIIGSALEIYQLGCRNIV----------------- 197
           + +Q++ FK Y  +L +I G  ++  II  A+ +   G  + V                 
Sbjct: 130 LYQQVEYFKEYKSKLIKIAGSKKADSIIKGAICLLSAGSSDFVQNYYVNPLLYKVYTVDA 189

Query: 198 -------------------------VAGLPPIGCLPIQETIAFENPLKRSCLEDQNSDSK 257
                                    V  LPP GCLP   T+   +  ++ C+   N+D++
Sbjct: 190 YGSFLIDNFSTFIKQVYAVGARKIGVTSLPPTGCLPAARTLFGFH--EKGCVSRLNTDAQ 249

Query: 258 AYNQKLSKLLNNLQPQLSGSKILYADIYNPLIDMVNNPKKYGFEQTNRGCCGTGLAE-AG 312
            +N+KL+   + LQ Q S  KI+  DIY+PL D+V NP K GF +  +GCCGTG  E   
Sbjct: 250 NFNKKLNAAASKLQKQYSDLKIVVFDIYSPLYDLVQNPSKSGFTEATKGCCGTGTVETTS 309

[Close]
BLAST of Lsi10G002060 vs. TAIR10
Match: AT3G43570.1 (AT3G43570.1 GDSL-like Lipase/Acylhydrolase superfamily protein)

HSP 1 Score: 218.0 bits (554), Expect = 8.4e-57
Identity = 118/317 (37.22%), Postives = 185/317 (58.36%), Query Frame = 1 

Query: 11  LSLHIIWL--LFLSKPCSALEPKTSRSFPAILIFGDSTVDTGNNNFIHTIFKGNYFPYGK 70
           + + IIWL  + +    +A++   + + PA+++FGDS +DTGNNN + T+ K N+ PYGK
Sbjct: 1   MKIQIIWLTLVLIVVEANAVKQGKNATIPALIVFGDSIMDTGNNNNLPTLLKCNFPPYGK 60

Query: 71  NFPGHVATGRFSDGKLIPDMVASKLGIKELVPPFLDPKLSNDDIKTGVSFASAGTGFDDL 130
           ++PG  ATGRFSDG++  D++A K+G+ + +P +++P L  +D+  GV+FAS GTG+D L
Sbjct: 61  DYPGGFATGRFSDGRVPSDLIAEKIGLAKTLPAYMNPYLKPEDLLKGVTFASGGTGYDPL 120

Query: 131 TAAISKVIPVMKQIDLFKNYIERLQRIVGVDESRRIIGSALEIYQLGCRNIVVAGLPPIG 190
           TA I  VI V  Q+  FK YI +++R  G ++++ I+  +  +      ++    L    
Sbjct: 121 TAKIMSVISVWDQLIYFKEYISKIKRHFGEEKAKDILEHSFFLVVSSSNDLAHTYLAQ-- 180

Query: 191 CLPIQETIAFENPLKRSCLEDQNSDSKAYNQKLSKL------LNNLQPQLSGSK-----I 250
                +  ++ N L  S +       K   QK+            LQ  + G K     I
Sbjct: 181 -AHRYDRTSYANFLADSAVHFVRELHKLGAQKIGVFSAVPVGCVPLQRTVFGDKELDGVI 240

Query: 251 LYADIYNPLIDMVNNPKKYGFEQTNRGCCGTGLAEAGPLCNRITP-TCENSSKFMFWDSI 310
           LY ++Y+ L DM+ +PKKYGFE  +RGCCG GL     LCN +   TC NSS ++FWDS 
Sbjct: 241 LYINVYDTLFDMIQHPKKYGFEVADRGCCGKGLLTISYLCNSLNQFTCSNSSAYIFWDSY 300

Query: 311 HPTEAAYKFIAESLLEK 314
           HP++ AY+ I ++LL+K
Sbjct: 301 HPSKRAYQVIVDNLLDK 314

[Close]
BLAST of Lsi10G002060 vs. NCBI nr
Match: gi|449466691|ref|XP_004151059.1| (PREDICTED: GDSL esterase/lipase At2g30310-like [Cucumis sativus])

HSP 1 Score: 433.0 bits (1112), Expect = 4.7e-118
Identity = 229/364 (62.91%), Postives = 255/364 (70.05%), Query Frame = 1 

Query: 1   MAGVNCLIVALSLHIIWLLF-LSKPCSALEPKTSRSFPAILIFGDSTVDTGNNNFIHTIF 60
           MA VNCLI ALSLH IWLL  L+KPCS+LEPKT+ SFPAILIFGDSTVDTGNNNFI TIF
Sbjct: 1   MARVNCLIAALSLHTIWLLVVLTKPCSSLEPKTTPSFPAILIFGDSTVDTGNNNFIPTIF 60

Query: 61  KGNYFPYGKNFPGHVATGRFSDGKLIPDMVASKLGI------------------------ 120
           KGNY PYGKNFPGH+ATGRFSDGKLIPDMVAS+LGI                        
Sbjct: 61  KGNYSPYGKNFPGHLATGRFSDGKLIPDMVASRLGIKELVPPFLDPKLSNDDIKTGVSFA 120

Query: 121 ---------KELVPPFLDPKLSNDDIKTGVSFASAGTGFDDLTAAISKVIPVMK------ 180
                       +   +      D  K  +       G D+    I+  + V+       
Sbjct: 121 SAGTGFDDLTAAISKVIPVMKQIDHFKNYIQRLQGVVGVDESKRIINNALVVISAGTNDL 180

Query: 181 QIDLFKNYIERLQ-RIVGVDE--SRRIIGSALEIYQLGCRNIVVAGLPPIGCLPIQETIA 240
            I+ +     +LQ  I G  +    R+     EIYQLGCRNIVVAGLPP+GCLPIQETIA
Sbjct: 181 NINFYDLPTRQLQYNISGYQDFLQNRLQSLIKEIYQLGCRNIVVAGLPPVGCLPIQETIA 240

Query: 241 FENPLKRSCLEDQNSDSKAYNQKLSKLLNNLQPQLSGSKILYADIYNPLIDMVNNPKKYG 300
           FENPLKR+CL+DQNSDS AYNQKLSKLL NLQPQL+GSKILYADIY PLIDM+NNP+KYG
Sbjct: 241 FENPLKRNCLKDQNSDSVAYNQKLSKLLTNLQPQLAGSKILYADIYTPLIDMLNNPQKYG 300

Query: 301 FEQTNRGCCGTGLAEAGPLCNRITPTCENSSKFMFWDSIHPTEAAYKFIAESLLEKLGDP 322
           F+ TNRGCCGTGL EAGPLCN  TPTCENSSKFMFWDSIHPTEAAYKFIAE+LL+KLGD 
Sbjct: 301 FDHTNRGCCGTGLVEAGPLCNPKTPTCENSSKFMFWDSIHPTEAAYKFIAEALLKKLGDT 360

[Close]
BLAST of Lsi10G002060 vs. NCBI nr
Match: gi|659127676|ref|XP_008463830.1| (PREDICTED: GDSL esterase/lipase At2g30310-like [Cucumis melo])

HSP 1 Score: 399.4 bits (1025), Expect = 5.8e-108
Identity = 217/362 (59.94%), Postives = 245/362 (67.68%), Query Frame = 1 

Query: 1   MAGVNCLIVALSLHIIWLLFLSKPCSALEPKTSRSFPAILIFGDSTVDTGNNNFIHTIFK 60
           MA     I ALSLH+IWLLFLSKPC+ALEPK +RSFP+ILIFGDSTVDTGNNNFI TIFK
Sbjct: 1   MARATYHIAALSLHMIWLLFLSKPCTALEPKITRSFPSILIFGDSTVDTGNNNFISTIFK 60

Query: 61  GNYFPYGKNFPGHVATGRFSDGKLIPDMVASKLGIKELVP-------------------- 120
            NY PYG +FPGHVATGRFSDGKLIPDMVASKLGIKELVP                    
Sbjct: 61  ANYSPYGADFPGHVATGRFSDGKLIPDMVASKLGIKELVPPFLDTKLSDDNVKTGVSFAS 120

Query: 121 -----PFLDPKLSN--------DDIKTGVSFASAGTGFDDLTAAISKVIPVMK------Q 180
                  L   +SN        D  K  +       G D+    ++  + ++        
Sbjct: 121 AGSGFDELTASVSNVIPVMKQIDMFKNYIRRLQGIVGVDESRKILNSALVIISAGTNDVN 180

Query: 181 IDLFKNYIERLQ-RIVGVDE--SRRIIGSALEIYQLGCRNIVVAGLPPIGCLPIQETIAF 240
           I+ +     +LQ  I G  +    R+     EIYQLGCR +VVAGLPP+GCLPIQE+IAF
Sbjct: 181 INFYDLPTRQLQYNISGYQDFLQDRLQSLIKEIYQLGCRTMVVAGLPPVGCLPIQESIAF 240

Query: 241 ENPLKRSCLEDQNSDSKAYNQKLSKLLNNLQPQLSGSKILYADIYNPLIDMVNNPKKYGF 300
           +NPL R CLE+QNSDSKAYNQKL+ LL+NLQPQL GS ILYADIY PLIDMVNNP  YGF
Sbjct: 241 QNPLDRKCLEEQNSDSKAYNQKLAHLLSNLQPQLPGSTILYADIYTPLIDMVNNPHNYGF 300

Query: 301 EQTNRGCCGTGLAEAGPLCNRITPT-CENSSKFMFWDSIHPTEAAYKFIAESLLEKLGDP 320
           E  N GCCGTG+AEAGPLCN  T T CEN SKFMFWDSIHPTEAAYKFI ESLL++  D 
Sbjct: 301 EHVNVGCCGTGMAEAGPLCNSKTSTICENPSKFMFWDSIHPTEAAYKFITESLLKQFADR 360

[Close]
BLAST of Lsi10G002060 vs. NCBI nr
Match: gi|659127674|ref|XP_008463828.1| (PREDICTED: GDSL esterase/lipase At2g30310-like [Cucumis melo])

HSP 1 Score: 399.1 bits (1024), Expect = 7.5e-108
Identity = 215/361 (59.56%), Postives = 241/361 (66.76%), Query Frame = 1 

Query: 1   MAGVNCLIVALSLHIIWLLFLSKPCSALEPKTSRSFPAILIFGDSTVDTGNNNFIHTIFK 60
           MA  N LI ALS+HIIWLL LSKPCSAL PKTSRSFPAILIFGDSTVDTGNNNFI TIFK
Sbjct: 1   MARANYLITALSIHIIWLLLLSKPCSALAPKTSRSFPAILIFGDSTVDTGNNNFISTIFK 60

Query: 61  GNYFPYGKNFPGHVATGRFSDGKLIPDMVASKLGIKE----------------------- 120
            NY+PYGK+FPG VATGRFSDGKLIPDMVASKLGIKE                       
Sbjct: 61  ANYWPYGKDFPGQVATGRFSDGKLIPDMVASKLGIKELVPPFLDPKLSDDDVKTGVSFAS 120

Query: 121 ----------LVPPFLDPKLSNDDIKTGVSFASAGTGFDDLTAAISKVIPVMK--QIDLF 180
                      +   +      D  K  +       G D+    I   + V+     DL 
Sbjct: 121 AGTGVDDLTAAISKVIPTMKQIDMFKNYIQRLQRIAGVDESKRIIGSALTVISVGTNDLT 180

Query: 181 KNYIE----RLQ-RIVGVDE--SRRIIGSALEIYQLGCRNIVVAGLPPIGCLPIQETIAF 240
            N+ +    +LQ  I G  E    R+     EIYQLGCR IVVAGLPPIGCLPIQETI+ 
Sbjct: 181 YNFYDIPTRQLQYNISGYQEFLQNRLQSLIKEIYQLGCRTIVVAGLPPIGCLPIQETISS 240

Query: 241 ENPLKRSCLEDQNSDSKAYNQKLSKLLNNLQPQLSGSKILYADIYNPLIDMVNNPKKYGF 300
             PL R CLE QN D++AYNQKLSKLL +LQPQL GS ILYADIY PL+DM+NNPKKYGF
Sbjct: 241 PIPLNRRCLEYQNKDAEAYNQKLSKLLGSLQPQLPGSTILYADIYTPLMDMINNPKKYGF 300

Query: 301 EQTNRGCCGTGLAEAGPLCNRITPTCENSSKFMFWDSIHPTEAAYKFIAESLLEKLGDPL 320
           EQTN GCCGTGL EAGPLCN++ PTCE+ SK+MFWDSIHP+EA YKF+ ESLL +  D L
Sbjct: 301 EQTNIGCCGTGLVEAGPLCNKVIPTCEDPSKYMFWDSIHPSEATYKFVTESLLNQFSDRL 360

[Close]
BLAST of Lsi10G002060 vs. NCBI nr
Match: gi|449450950|ref|XP_004143225.1| (PREDICTED: GDSL esterase/lipase At2g30310 [Cucumis sativus])

HSP 1 Score: 379.4 bits (973), Expect = 6.2e-102
Identity = 208/361 (57.62%), Postives = 243/361 (67.31%), Query Frame = 1 

Query: 1   MAGVNCLIVALSLHIIWLLFLSKPCSALEPKTSRSFPAILIFGDSTVDTGNNNFIHTIFK 60
           MA  N LI ALS+HIIWLLFLSKPCSAL PKTSRSF ++LIFGDSTVDTGNNNFI TIFK
Sbjct: 1   MARANYLIAALSIHIIWLLFLSKPCSALAPKTSRSFSSVLIFGDSTVDTGNNNFIPTIFK 60

Query: 61  GNYFPYGKNFPGHVATGRFSDGKLI-------------------PDM-----------VA 120
            NY+PYGK+FPGHVATGRFSDGKLI                   P++            +
Sbjct: 61  ANYWPYGKDFPGHVATGRFSDGKLIPDMVASKLGIKELVPPFLDPELSDDDVKTGVSFAS 120

Query: 121 SKLGIKELVPPF--LDPKLSNDDI-KTGVSFASAGTGFDDLTAAISKVIPVMK--QIDLF 180
           +  G+ +L      + P +   D+ K  +       G D+    I   + V+     DL 
Sbjct: 121 AGTGVDDLTAAISKVIPAMKQIDMFKNYIQRLQRIVGVDESKRIIGSALAVISVGTNDLT 180

Query: 181 KNYIE----RLQ-RIVGVDE--SRRIIGSALEIYQLGCRNIVVAGLPPIGCLPIQETIAF 240
            N+ +    +LQ  I G  E    R+     EIYQLGCR IVVAGLPPIGCLPIQETI+ 
Sbjct: 181 FNFYDIPTRQLQYNISGYQEFLQNRLQSLIKEIYQLGCRTIVVAGLPPIGCLPIQETISS 240

Query: 241 ENPLKRSCLEDQNSDSKAYNQKLSKLLNNLQPQLSGSKILYADIYNPLIDMVNNPKKYGF 300
             PL R CLE QN D++AYNQKLSKLL +LQPQL GS+ILYADIY PL+DM+NNP+KYGF
Sbjct: 241 PIPLNRRCLEYQNKDAEAYNQKLSKLLGSLQPQLPGSQILYADIYTPLMDMINNPQKYGF 300

Query: 301 EQTNRGCCGTGLAEAGPLCNRITPTCENSSKFMFWDSIHPTEAAYKFIAESLLEKLGDPL 320
           EQTN GCCGTGL EAGPLCN+ITPTCE+ SKFMFWDSIHP+EA YKF+ ESLL +  D L
Sbjct: 301 EQTNIGCCGTGLVEAGPLCNKITPTCEDPSKFMFWDSIHPSEATYKFVTESLLNQFFDRL 360

[Close]
BLAST of Lsi10G002060 vs. NCBI nr
Match: gi|976906698|gb|KVH94108.1| (Lipase, GDSL [Cynara cardunculus var. scolymus])

HSP 1 Score: 376.7 bits (966), Expect = 4.0e-101
Identity = 169/306 (55.23%), Postives = 238/306 (77.78%), Query Frame = 1 

Query: 13  LHIIWLLFLSKPCSALEPKTSRSFPAILIFGDSTVDTGNNNFIHTIFKGNYFPYGKNFPG 72
           LH+ + L L +         +  FPAIL+FGDSTVDTGNNN+I T+ K N++PYGK+FPG
Sbjct: 2   LHLPFFLLLLQVLPQKTNGQNFKFPAILVFGDSTVDTGNNNYITTLSKANHYPYGKDFPG 61

Query: 73  HVATGRFSDGKLIPDMVASKLGIKELVPPFLDPKLSNDDIKTGVSFASAGTGFDDLTAAI 132
           HV TGRFS+GKL  D++AS LG+K+ +PPFL P LS  +++TGV FASAGTG+D LTAA+
Sbjct: 62  HVPTGRFSNGKLAVDLLASLLGVKQTIPPFLQPDLSAYELQTGVCFASAGTGYDRLTAAL 121

Query: 133 SKVIPVMKQIDLFKNYIERLQRIVGVDESRRIIGSALEIYQLGCRNIVVAGLPPIGCLPI 192
           ++VIP+ +Q++ FK+Y ERL+++ G + ++RIIG ALE+Y LGCR +++ GLPPIGCLPI
Sbjct: 122 TRVIPMSQQLNYFKSYRERLKKMAGEEAAQRIIGQALELYNLGCRTLIITGLPPIGCLPI 181

Query: 193 QETIAFENPLKRSCLEDQNSDSKAYNQKLSKLLNNLQPQLSGSKILYADIYNPLIDMVNN 252
           Q T  F+    R+C+E+QN D +AYN+KL +LL ++Q  L GSKI YADIY P++DM+ +
Sbjct: 182 QITAKFKGQFGRTCVEEQNVDGQAYNRKLIELLPHIQSSLEGSKIFYADIYKPIMDMIKS 241

Query: 253 PKKYGFEQTNRGCCGTGLAEAGPLCNRITPTCENSSKFMFWDSIHPTEAAYKFIAESLLE 312
           P+K+GF +T +GCCGTGL EAGP+C  +TP C+NSS+++F+DSIHP+E AY+++ E L +
Sbjct: 242 PEKHGFRETMKGCCGTGLLEAGPICTPLTPLCQNSSEYLFFDSIHPSEKAYRYVTECLKK 301

Query: 313 KLGDPL 319
           ++ D L
Sbjct: 302 QILDKL 307

The following BLAST results are available for this feature:
BLAST of Lsi10G002060 vs. Swiss-Prot
Match NameE-valueIdentityDescription
GDL41_ARATH7.4e-7946.94GDSL esterase/lipase At2g30220 OS=Arabidopsis thaliana GN=At2g30220 PE=3 SV=1[more]
GDL43_ARATH2.9e-7544.35GDSL esterase/lipase At2g31540 OS=Arabidopsis thaliana GN=At2g31540 PE=2 SV=1[more]
GDL44_ARATH6.4e-7544.79GDSL esterase/lipase At2g31550 OS=Arabidopsis thaliana GN=At2g31550 PE=3 SV=3[more]
GDL1_ARATH4.5e-6841.80GDSL esterase/lipase At1g06990 OS=Arabidopsis thaliana GN=At1g06990 PE=2 SV=2[more]
APG2_ARATH3.2e-5836.09GDSL esterase/lipase APG OS=Arabidopsis thaliana GN=APG PE=2 SV=1[more]
BLAST of Lsi10G002060 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LNE2_CUCSA3.3e-11862.91Uncharacterized protein OS=Cucumis sativus GN=Csa_2G431080 PE=4 SV=1[more]
A0A0A0KF79_CUCSA4.3e-10257.62Uncharacterized protein OS=Cucumis sativus GN=Csa_6G180980 PE=4 SV=1[more]
A0A103XNX7_CYNCS2.8e-10155.23Lipase, GDSL OS=Cynara cardunculus var. scolymus GN=Ccrd_003829 PE=4 SV=1[more]
M1DB51_SOLTU6.4e-9855.70Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400035739 PE=4 SV=1[more]
A0A0A0KC28_CUCSA8.4e-9055.26Uncharacterized protein OS=Cucumis sativus GN=Csa_6G180990 PE=4 SV=1[more]
BLAST of Lsi10G002060 vs. TAIR10
Match NameE-valueIdentityDescription
AT2G30220.14.1e-8046.94 GDSL-like Lipase/Acylhydrolase family protein[more]
AT2G31540.11.6e-7644.35 GDSL-like Lipase/Acylhydrolase superfamily protein[more]
AT1G06990.12.5e-6941.80 GDSL-like Lipase/Acylhydrolase superfamily protein[more]
AT3G16370.11.8e-5936.09 GDSL-like Lipase/Acylhydrolase superfamily protein[more]
AT3G43570.18.4e-5737.22 GDSL-like Lipase/Acylhydrolase superfamily protein[more]
BLAST of Lsi10G002060 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449466691|ref|XP_004151059.1|4.7e-11862.91PREDICTED: GDSL esterase/lipase At2g30310-like [Cucumis sativus][more]
gi|659127676|ref|XP_008463830.1|5.8e-10859.94PREDICTED: GDSL esterase/lipase At2g30310-like [Cucumis melo][more]
gi|659127674|ref|XP_008463828.1|7.5e-10859.56PREDICTED: GDSL esterase/lipase At2g30310-like [Cucumis melo][more]
gi|449450950|ref|XP_004143225.1|6.2e-10257.62PREDICTED: GDSL esterase/lipase At2g30310 [Cucumis sativus][more]
gi|976906698|gb|KVH94108.1|4.0e-10155.23Lipase, GDSL [Cynara cardunculus var. scolymus][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0016788hydrolase activity, acting on ester bonds
Vocabulary: INTERPRO
TermDefinition
IPR001087GDSL
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
biological_process GO:0008152 metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0016788 hydrolase activity, acting on ester bonds
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi10G002060.1Lsi10G002060.1mRNA


InterPro
Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001087GDSL lipase/esterasePFAMPF00657Lipase_GDSLcoord: 157..309
score: 5.5
NoneNo IPR availableunknownCoilCoilcoord: 207..227
scor
NoneNo IPR availablePANTHERPTHR22835ZINC FINGER FYVE DOMAIN CONTAINING PROTEINcoord: 8..314
score: 1.6E
NoneNo IPR availablePANTHERPTHR22835:SF152SUBFAMILY NOT NAMEDcoord: 8..314
score: 1.6E