Lsi10G001840 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi10G001840
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Terpene synthase
Location	chr10 : 3054239 .. 3055833 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTATTCTTATAAAGGTAATGCAGGAAGAAGATGAGATGGTCAAGAAAGAACTTGAAGAACTGAAAGAAGGAGTGAGAAGCATGTTTGCAGCTGCTGAAAAGTATTCAGAAAAATTAGGTTTGATTGATTCAATCCAACGCTTAGGTTTGTCATACCATTTTGAAGATGAAATTAATGAAATTCTTGATCAAATAAAAATTTCTGCCAATGATGACGACAAAGACGAGGATCTTTACATTATGGCTCTTAAATTTCGATTGCTTAGACAACAAGGTTTCTATGTTTCTTCTGGTAAGCCTCTCTCATGCAATTTTTACCATGAATTGTTGCTATAAGAAGATTGAAATATTAAATTCAATTTAAATTTTGTATTTTTAATAAATCTTTGATTTATGAGATCTTATAAGGCCCTAGTTCAACAAAATAATAAATCTTTGATTTGAGGTAAGGAATGGAGTTATGAAGTCTAGGGAGTTGTGAACTCTCGAGACTCACAATGGAAGAAGATAATAAAATATAAAATTGATATTTTTTAGTAGTGAGACCCATCAACTCATTGGGTCAAACAAGGAGTGGAGTTCAGAACTCTACTTCCCCTGACAACTCCTTGGGCCAAACACCACCTAAAAAAATTTATAATTACTACTTATACTAACAAGGTGCACCATTTTTTTTTTATTGTTTCATCATAGGAAGTCATGTATTCATACCTCTGTGATATCATTTCCCCACCAATTAATATTTTGATTTCTACTTGTACGATGATCTTTTTAATATTTACGGATCTATTAGATATAGTAATGAAAATTGAAATTTAAAGTTTATGGATTCATATTAGTGACAATAATTTCCATAAATGAGGTTGTAATTTAAGCATTGTAAATTTCAATAGAGAGATTCAATAAGTTAAGAAATGAAAGAGGAGATTTCAAAGAATCAATTACAAAGGATGAAAGTGGTTTATTAAGTTTGTGAGGCATCTCACTTGAGAATGAAGGGAGAAAGTATATTAGATGAAGCATTAGCTTTCACAACTACTCAACTTGAAGCAATGACCATGGATTCCACTTCTCAATTCTCAGAAGAAGCTAAATATGCATTGAAGTGGCCAATCTACAAAGCTATGCCAAGGTTGACGAGTAGGCACTATATGTCTCTCTATCACAACAATCCATTGAAGAACAATAATATGTTGCTCACTTTTGCAAAGTTAGACTACAATTCTCTTCAAAAACTCTACCAAAAAGAACTAGGTGAACTTTCAAGGTAACTTTTTCAATAAGCTTTCTTTGTTTTTTTTTGTTTTGTTAATGTTCTTATTATGGGTGTGAATTAATATTATAGGTGGTGGAAGGATCTAAAGTTAAGGGAGGAATTACCTTTTGCACGAGATAGGATGGTGGAGAGCTATATTTGGGCATTGGGAATATACTATGAACCAAAGTACTCTAGTGCTAGAATAATATCAGCCAAAGTTATTGTTTTAATGACGGTTCTTGATGATATATATGATTCCTATGGCACACTAGAAGAACTTCAACTATTCACCAATGCAATTGAAATGTTTAGTTCTTCAACTCCTTCTTTTTAA

mRNA sequence

ATGTCTATTCTTATAAAGGTAATGCAGGAAGAAGATGAGATGGTCAAGAAAGAACTTGAAGAACTGAAAGAAGGAGTGAGAAGCATGTTTGCAGCTGCTGAAAAGTATTCAGAAAAATTAGGTTTGATTGATTCAATCCAACGCTTAGGTTTGTCATACCATTTTGAAGATGAAATTAATGAAATTCTTGATCAAATAAAAATTTCTGCCAATGATGACGACAAAGACGAGGATCTTTACATTATGGCTCTTAAATTTCGATTGCTTAGACAACAAGGTTTCTATGTTTCTTCTGAAGAAGCTAAATATGCATTGAAGTGGCCAATCTACAAAGCTATGCCAAGGTTGACGAGTAGGCACTATATGTCTCTCTATCACAACAATCCATTGAAGAACAATAATATGTTGCTCACTTTTGCAAAGTTAGACTACAATTCTCTTCAAAAACTCTACCAAAAAGAACTAGGTGAACTTTCAAGGTGGTGGAAGGATCTAAAGTTAAGGGAGGAATTACCTTTTGCACGAGATAGGATGGTGGAGAGCTATATTTGGGCATTGGGAATATACTATGAACCAAAGTACTCTAGTGCTAGAATAATATCAGCCAAAGTTATTGTTTTAATGACGGTTCTTGATGATATATATGATTCCTATGGCACACTAGAAGAACTTCAACTATTCACCAATGCAATTGAAATGTTTAGTTCTTCAACTCCTTCTTTTTAA

Coding sequence (CDS)

ATGTCTATTCTTATAAAGGTAATGCAGGAAGAAGATGAGATGGTCAAGAAAGAACTTGAAGAACTGAAAGAAGGAGTGAGAAGCATGTTTGCAGCTGCTGAAAAGTATTCAGAAAAATTAGGTTTGATTGATTCAATCCAACGCTTAGGTTTGTCATACCATTTTGAAGATGAAATTAATGAAATTCTTGATCAAATAAAAATTTCTGCCAATGATGACGACAAAGACGAGGATCTTTACATTATGGCTCTTAAATTTCGATTGCTTAGACAACAAGGTTTCTATGTTTCTTCTGAAGAAGCTAAATATGCATTGAAGTGGCCAATCTACAAAGCTATGCCAAGGTTGACGAGTAGGCACTATATGTCTCTCTATCACAACAATCCATTGAAGAACAATAATATGTTGCTCACTTTTGCAAAGTTAGACTACAATTCTCTTCAAAAACTCTACCAAAAAGAACTAGGTGAACTTTCAAGGTGGTGGAAGGATCTAAAGTTAAGGGAGGAATTACCTTTTGCACGAGATAGGATGGTGGAGAGCTATATTTGGGCATTGGGAATATACTATGAACCAAAGTACTCTAGTGCTAGAATAATATCAGCCAAAGTTATTGTTTTAATGACGGTTCTTGATGATATATATGATTCCTATGGCACACTAGAAGAACTTCAACTATTCACCAATGCAATTGAAATGTTTAGTTCTTCAACTCCTTCTTTTTAA

Protein sequence

MSILIKVMQEEDEMVKKELEELKEGVRSMFAAAEKYSEKLGLIDSIQRLGLSYHFEDEINEILDQIKISANDDDKDEDLYIMALKFRLLRQQGFYVSSEEAKYALKWPIYKAMPRLTSRHYMSLYHNNPLKNNNMLLTFAKLDYNSLQKLYQKELGELSRWWKDLKLREELPFARDRMVESYIWALGIYYEPKYSSARIISAKVIVLMTVLDDIYDSYGTLEELQLFTNAIEMFSSSTPSF

Homology

[Close]

BLAST of Lsi10G001840 vs. Swiss-Prot
Match: TPSGD_VITVI ((-)-germacrene D synthase OS=Vitis vinifera GN=VIT_19s0014g04930 PE=1 SV=1)

HSP 1 Score: 158.7 bits (400), Expect = 8.1e-38
Identity = 81/166 (48.80%), Postives = 109/166 (65.66%), Query Frame = 1


Query: 72  DDDKDEDLYIMALKFRLLRQQGFYVSSEEAKYALKWPIYKAMPRLTSRHYMSLYHNNPLK 131
           +D  DE L       +   +      +E+  +ALK PI K +PRL +RHY S+Y  +   
Sbjct: 171 EDILDEALAFTTTHLQSATKHSSNPLAEQVVHALKQPIRKGLPRLEARHYFSVYQADD-S 230

Query: 132 NNNMLLTFAKLDYNSLQKLYQKELGELSRWWKDLKLREELPFARDRMVESYIWALGIYYE 191
           +N  LL  AKLD+N LQKL+QKEL ++S WWKDL    +LPFARDR+VE Y W LG+Y+E
Sbjct: 231 HNKALLKLAKLDFNLLQKLHQKELSDISAWWKDLDFAHKLPFARDRVVECYFWILGVYFE 290

Query: 192 PKYSSARIISAKVIVLMTVLDDIYDSYGTLEELQLFTNAIEMFSSS 238
           P++  AR I  KVI + +++DDIYD YGTLEEL+LFT A+E +  S
Sbjct: 291 PQFFFARRILTKVIAMTSIIDDIYDVYGTLEELELFTEAVERWDIS 335

[Close]

BLAST of Lsi10G001840 vs. Swiss-Prot
Match: GCSY1_CUCME ((+)-gamma-cadinene synthase OS=Cucumis melo PE=1 SV=1)

HSP 1 Score: 154.8 bits (390), Expect = 1.2e-36
Identity = 74/140 (52.86%), Postives = 100/140 (71.43%), Query Frame = 1


Query: 98  SEEAKYALKWPIYKAMPRLTSRHYMSLYHNNPLKNNNMLLTFAKLDYNSLQKLYQKELGE 157
           + E   ALK PI K++PR+ +R Y+ +Y  +P  +N  LL F+KLD+N LQKL+QKEL E
Sbjct: 210 ASEVSNALKLPIRKSVPRIKAREYLEIYQQHP-SHNETLLEFSKLDFNILQKLHQKELSE 269

Query: 158 LSRWWKDLKLREELPFARDRMVESYIWALGIYYEPKYSSARIISAKVIVLMTVLDDIYDS 217
           + RWWKDL +  + PFARDR+VE Y W LG Y+EP+YS  R +  KVI + ++LDDIYD+
Sbjct: 270 ICRWWKDLDVPTKFPFARDRIVECYFWTLGAYFEPQYSVGRKMLTKVIAIASILDDIYDA 329

Query: 218 YGTLEELQLFTNAIEMFSSS 238
           YGT EELQ+ T AI+ +  S
Sbjct: 330 YGTFEELQVLTPAIQRWDRS 348

[Close]

BLAST of Lsi10G001840 vs. Swiss-Prot
Match: TPGAS_POGCB (Germacrene A synthase OS=Pogostemon cablin PE=1 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 2.0e-36
Identity = 72/134 (53.73%), Postives = 101/134 (75.37%), Query Frame = 1


Query: 99  EEAKYALKWPIYKAMPRLTSRHYMSLYHNNPLKNNNMLLTFAKLDYNSLQKLYQKELGEL 158
           ++ ++AL   ++  +PR+ +RH+++ Y     KN  MLL FAKLD+N+LQ L+++EL E+
Sbjct: 194 KQVEHALFQHLHFGIPRVEARHFITFYEEEEHKNE-MLLRFAKLDFNALQALHKEELSEI 253

Query: 159 SRWWKDLKLREELPFARDRMVESYIWALGIYYEPKYSSARIISAKVIVLMTVLDDIYDSY 218
           S+WWKDL L  +LP+ARDR+VESY WA+G+YY+PKYS ARI+  K I +  +LDD YDSY
Sbjct: 254 SKWWKDLDLISKLPYARDRVVESYFWAVGVYYQPKYSRARIMLTKTIAMTAILDDTYDSY 313

Query: 219 GTLEELQLFTNAIE 233
           GTLEEL + T AIE
Sbjct: 314 GTLEELDVLTKAIE 326

[Close]

BLAST of Lsi10G001840 vs. Swiss-Prot
Match: CARS_ARTAN (Beta-caryophyllene synthase OS=Artemisia annua GN=QHS1 PE=1 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 1.7e-35
Identity = 75/132 (56.82%), Postives = 95/132 (71.97%), Query Frame = 1


Query: 104 ALKWPIYKAMPRLTSRHYMSLYHNNPLKNNNMLLTFAKLDYNSLQKLYQKELGELSRWWK 163
           ALK P++K + RL +  Y+ +Y      +N  LL  AKL +N LQ L++KEL E+SRWWK
Sbjct: 194 ALKQPLHKRLTRLEALRYIPMYEQQA-SHNESLLKLAKLGFNLLQSLHRKELSEVSRWWK 253

Query: 164 DLKLREELPFARDRMVESYIWALGIYYEPKYSSARIISAKVIVLMTVLDDIYDSYGTLEE 223
            L +   LP+ARDRMVE Y WALG+Y+EPKYS ARI  AKVI L TVLDD YD+YGT EE
Sbjct: 254 GLDVPNNLPYARDRMVECYFWALGVYFEPKYSQARIFLAKVISLATVLDDTYDAYGTYEE 313

Query: 224 LQLFTNAIEMFS 236
           L++FT AI+ +S
Sbjct: 314 LKIFTEAIQRWS 324

[Close]

BLAST of Lsi10G001840 vs. Swiss-Prot
Match: 5EAS_CAPAN (5-epiaristolochene synthase OS=Capsicum annuum GN=EAS PE=1 SV=1)

HSP 1 Score: 149.8 bits (377), Expect = 3.7e-35
Identity = 65/138 (47.10%), Postives = 102/138 (73.91%), Query Frame = 1


Query: 95  YVSSEEAKYALKWPIYKAMPRLTSRHYMSLYHNNPLKNNNMLLTFAKLDYNSLQKLYQKE 154
           Y   E+ ++AL+  ++K +PR+  + ++S  ++     N++LL FAKLDYN LQ L+++E
Sbjct: 195 YPLKEQVRHALEQSLHKGIPRIEIQFFISSVYDKQAIKNDVLLRFAKLDYNMLQMLHKQE 254

Query: 155 LGELSRWWKDLKLREELPFARDRMVESYIWALGIYYEPKYSSARIISAKVIVLMTVLDDI 214
           L E+SRWWKDL     LP+ARDR+VE Y WALG+YYEP+YS AR++  K I +++++DD 
Sbjct: 255 LAEVSRWWKDLNFVNTLPYARDRVVECYFWALGVYYEPQYSQARVMLVKTIAMISIVDDT 314

Query: 215 YDSYGTLEELQLFTNAIE 233
           YD+YGT++EL ++T+ I+
Sbjct: 315 YDAYGTVDELAIYTDVIQ 332

[Close]

BLAST of Lsi10G001840 vs. TrEMBL
Match: A0A0A0L791_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G042380 PE=3 SV=1)

HSP 1 Score: 276.2 bits (705), Expect = 3.9e-71
Identity = 146/241 (60.58%), Postives = 178/241 (73.86%), Query Frame = 1


Query: 29  MFAAAEKYSEKLGLIDSIQRLGLSYHFEDEINEI-LDQIKI--SANDDDKDEDLYIMALK 88
           MFAAAE  S++L LIDSIQRLGLS+HF++EINE+ L+QI+     ND+D DE+LY++AL+
Sbjct: 1   MFAAAENSSKRLSLIDSIQRLGLSHHFQEEINEVVLEQIEKLRKVNDEDADENLYLVALR 60

Query: 89  FRLLRQQGFYVSS-----------------------------EEAKYALKWPIYKAMPRL 148
           FRLLRQQ  ++ +                             EEAKYALKWPIYKA+PR 
Sbjct: 61  FRLLRQQASHLRTKGENILDEALAFTTTHLKAMAMDSNSPFFEEAKYALKWPIYKAVPRF 120

Query: 149 TSRHYMSLYHNNPLKNNNMLLTFAKLDYNSLQKLYQKELGELSRWWKDLKLREELPFARD 208
            +R Y+SLYHNNPLKNNN+LLTFAKLDYNSLQKLY KELGE SRWWKD KL+E LPFAR 
Sbjct: 121 MARQYISLYHNNPLKNNNVLLTFAKLDYNSLQKLYHKELGEFSRWWKDHKLKELLPFARV 180

Query: 209 RMVESYIWALGIYYEPKYSSARIISAKVIVLMTVLDDIYDSYGTLEELQLFTNAIEMFSS 238
           R+VE Y+WALGI YEPKYS+ R I  K+I  +++LDD+YD Y TL+ELQLFTNAI+ +  
Sbjct: 181 RVVEIYVWALGIQYEPKYSNTRRILVKIITFISLLDDMYDVYATLDELQLFTNAIQRWDV 240

[Close]

BLAST of Lsi10G001840 vs. TrEMBL
Match: A0A0A0LN25_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G427840 PE=3 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 6.4e-58
Identity = 112/157 (71.34%), Postives = 130/157 (82.80%), Query Frame = 1


Query: 76  DEDLYIMALKFRLLRQQGFYVSSEEAKYALKWPIYKAMPRLTSRHYMSLYHNNPLKNNNM 135
           DE L   + +   +        SEEAKYALKWPIYK +PR  +R+Y+SLY NNPLK +N+
Sbjct: 181 DEALEFTSTQLEAMAMDSTSPFSEEAKYALKWPIYKTLPRFMTRNYISLYRNNPLKKSNI 240

Query: 136 LLTFAKLDYNSLQKLYQKELGELSRWWKDLKLREELPFARDRMVESYIWALGIYYEPKYS 195
           LLTFAKLDYNSLQKLYQ+ELGELSRWW DLKL+E+LPFARDR+VESYIWALGIYYEPKYS
Sbjct: 241 LLTFAKLDYNSLQKLYQRELGELSRWWNDLKLKEKLPFARDRVVESYIWALGIYYEPKYS 300

Query: 196 SARIISAKVIVLMTVLDDIYDSYGTLEELQLFTNAIE 233
           SAR I AK+I ++TV+DD+YD YGTLEELQLFT AIE
Sbjct: 301 SARTIVAKIIAIITVIDDMYDCYGTLEELQLFTKAIE 337

[Close]

BLAST of Lsi10G001840 vs. TrEMBL
Match: A0A0A0L2M0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G040870 PE=3 SV=1)

HSP 1 Score: 222.6 bits (566), Expect = 5.1e-55
Identity = 112/172 (65.12%), Postives = 134/172 (77.91%), Query Frame = 1


Query: 75  KDEDLYIMALKFRLLRQQGFYVSS-----EEAKYALKWPIYKAMPRLTSRHYMSLYHNNP 134
           K E++   AL F   + Q   + S     +E K++LKWPIYKAMPR  SRHY+SLY NNP
Sbjct: 121 KGENILDKALAFTTTQLQAIAMDSNSPFSQEVKFSLKWPIYKAMPRFMSRHYISLYQNNP 180

Query: 135 LKNNNMLLTFAKLDYNSLQKLYQKELGELSRWWKDLKLREELPFARDRMVESYIWALGIY 194
           LK+N +LLTFAKLDYNSLQKLYQKELGE SRWWKD+ LRE+L FARDR VE Y WA+G+Y
Sbjct: 181 LKDN-VLLTFAKLDYNSLQKLYQKELGEFSRWWKDMMLREQLCFARDRAVECYTWAMGVY 240

Query: 195 YEPKYSSARIISAKVIVLMTVLDDIYDSYGTLEELQLFTNAIEMFSSSTPSF 242
           YEPKYSS RI+ AKVI  +++LDD+YD+Y T EELQLFT++IE F SST SF
Sbjct: 241 YEPKYSSGRILLAKVIAFLSILDDMYDAYATFEELQLFTHSIERFCSSTISF 291

[Close]

BLAST of Lsi10G001840 vs. TrEMBL
Match: A0A0B4VFP0_CUCME (Sesquiterpene synthase Tps3 OS=Cucumis melo PE=2 SV=1)

HSP 1 Score: 202.6 bits (514), Expect = 5.4e-49
Identity = 98/138 (71.01%), Postives = 118/138 (85.51%), Query Frame = 1


Query: 99  EEAKYALKWPIYKAMPRLTSRHYMSLYHNNPLKNNNMLLTFAKLDYNSLQKLYQKELGEL 158
           +EAKYAL+WPI KA+PRL ++H++S Y  +PLKNN +LL FAKLDYN++QKLYQKEL E+
Sbjct: 161 DEAKYALRWPILKALPRLITKHHISTYEKDPLKNN-VLLKFAKLDYNAMQKLYQKELYEV 220

Query: 159 SRWWKDLKLREELPFARDRMVESYIWALGIYYEPKYSSARIISAKVIVLMTVLDDIYDSY 218
           SRWWKDLKL +EL FARDRMVESYIWALG++YEPKYS  RII AK+ VL  +LDDIYD Y
Sbjct: 221 SRWWKDLKLMKELYFARDRMVESYIWALGVFYEPKYSYGRIILAKIAVLTILLDDIYDVY 280

Query: 219 GTLEELQLFTNAIEMFSS 237
           GT +EL+LFTNAIE + S
Sbjct: 281 GTFDELELFTNAIERWDS 297

[Close]

BLAST of Lsi10G001840 vs. TrEMBL
Match: A0A0A0L1X6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G039850 PE=3 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 2.3e-47
Identity = 99/157 (63.06%), Postives = 119/157 (75.80%), Query Frame = 1


Query: 76  DEDLYIMALKFRLLRQQGFYVSSEEAKYALKWPIYKAMPRLTSRHYMSLYHNNPLKNNNM 135
           D+ L     K + +         +EA+YALKWPI KA+PRL ++H +S Y  +PLK N +
Sbjct: 176 DKALAFTTTKLQEMAMDSTSPFGDEAEYALKWPILKALPRLITKHQISTYDKDPLKIN-V 235

Query: 136 LLTFAKLDYNSLQKLYQKELGELSRWWKDLKLREELPFARDRMVESYIWALGIYYEPKYS 195
           LL FAKLDYN+ QKLYQKEL E+SRWWKDLKL +EL FARDRMVESYIWALG++YEPKYS
Sbjct: 236 LLKFAKLDYNATQKLYQKELCEVSRWWKDLKLMKELSFARDRMVESYIWALGVFYEPKYS 295

Query: 196 SARIISAKVIVLMTVLDDIYDSYGTLEELQLFTNAIE 233
             RII AK+IVL TVLDD+YD Y T +EL+LFTN IE
Sbjct: 296 YGRIILAKIIVLATVLDDVYDIYATFDELELFTNVIE 331

[Close]

BLAST of Lsi10G001840 vs. TAIR10
Match: AT3G25830.1 (AT3G25830.1 terpene synthase-like sequence-1,8-cineole)

HSP 1 Score: 126.7 bits (317), Expect = 1.9e-29
Identity = 63/134 (47.01%), Postives = 90/134 (67.16%), Query Frame = 1


Query: 99  EEAKYALKWPIYKAMPRLTSRHYMSLYHNNPLKNNNMLLTFAKLDYNSLQKLYQKELGEL 158
           E+A +AL+ P +  M RL +R Y+  Y       N +L+ FAK+D+N +Q  +Q++L  +
Sbjct: 230 EKAYHALEMPYHWRMRRLDTRWYIDAYEKKH-DMNLVLIEFAKIDFNIVQAAHQEDLKYV 289

Query: 159 SRWWKDLKLREELPFARDRMVESYIWALGIYYEPKYSSARIISAKVIVLMTVLDDIYDSY 218
           SRWWKD  L  +LPF RDR+VE+Y W +G+ YEP++   R I   V  L+T +DDIYD Y
Sbjct: 290 SRWWKDTCLTNQLPFVRDRIVENYFWTVGLIYEPQFGYIRRIMTIVNALVTTIDDIYDIY 349

Query: 219 GTLEELQLFTNAIE 233
           GTLEEL+LFT+ +E
Sbjct: 350 GTLEELELFTSMVE 362

[Close]

BLAST of Lsi10G001840 vs. TAIR10
Match: AT3G25820.1 (AT3G25820.1 terpene synthase-like sequence-1,8-cineole)

HSP 1 Score: 126.7 bits (317), Expect = 1.9e-29
Identity = 63/134 (47.01%), Postives = 90/134 (67.16%), Query Frame = 1


Query: 99  EEAKYALKWPIYKAMPRLTSRHYMSLYHNNPLKNNNMLLTFAKLDYNSLQKLYQKELGEL 158
           E+A +AL+ P +  M RL +R Y+  Y       N +L+ FAK+D+N +Q  +Q++L  +
Sbjct: 230 EKAYHALEMPYHWRMRRLDTRWYIDAYEKKH-DMNLVLIEFAKIDFNIVQAAHQEDLKYV 289

Query: 159 SRWWKDLKLREELPFARDRMVESYIWALGIYYEPKYSSARIISAKVIVLMTVLDDIYDSY 218
           SRWWKD  L  +LPF RDR+VE+Y W +G+ YEP++   R I   V  L+T +DDIYD Y
Sbjct: 290 SRWWKDTCLTNQLPFVRDRIVENYFWTVGLIYEPQFGYIRRIMTIVNALVTTIDDIYDIY 349

Query: 219 GTLEELQLFTNAIE 233
           GTLEEL+LFT+ +E
Sbjct: 350 GTLEELELFTSMVE 362

[Close]

BLAST of Lsi10G001840 vs. TAIR10
Match: AT4G16730.1 (AT4G16730.1 terpene synthase 02)

HSP 1 Score: 126.3 bits (316), Expect = 2.5e-29
Identity = 61/134 (45.52%), Postives = 90/134 (67.16%), Query Frame = 1


Query: 99  EEAKYALKWPIYKAMPRLTSRHYMSLYHNNPLKNNNMLLTFAKLDYNSLQKLYQKELGEL 158
           E  K AL+ P + ++ RL +R Y+++Y     + N +LL FA +D+N LQ  +Q+EL  +
Sbjct: 177 EMVKRALETPYHWSIRRLEARWYINVYEKKH-EMNPLLLEFAAIDFNMLQANHQEELKLI 236

Query: 159 SRWWKDLKLREELPFARDRMVESYIWALGIYYEPKYSSARIISAKVIVLMTVLDDIYDSY 218
           S WW    L ++L F RDR+ ESY W +GI+YEP++   R I  K+ +L+ ++DDIYD Y
Sbjct: 237 SSWWNSTGLMKQLDFVRDRITESYFWTIGIFYEPEFKYCRKILTKIFMLIVIMDDIYDIY 296

Query: 219 GTLEELQLFTNAIE 233
           GTLEEL+LFTN +E
Sbjct: 297 GTLEELELFTNVVE 309

[Close]

BLAST of Lsi10G001840 vs. TAIR10
Match: AT5G23960.1 (AT5G23960.1 terpene synthase 21)

HSP 1 Score: 124.8 bits (312), Expect = 7.3e-29
Identity = 58/138 (42.03%), Postives = 96/138 (69.57%), Query Frame = 1


Query: 102 KYALKWPIYKAMPRLTSRHYMSLYHNNPLKNNNMLLTFAKLDYNSLQKLYQKELGELSRW 161
           K ALK P +K + R+ +R Y+S Y       +  LL FAK+D+N LQ L+++EL  ++RW
Sbjct: 190 KNALKHPYHKGISRIETRQYISYYEEEE-SCDPTLLEFAKIDFNLLQILHREELACVTRW 249

Query: 162 WKDLKLREELPFARDRMVESYIWALGIYYEPKYSSARIISAKVIVLMTVLDDIYDSYGTL 221
             +++ + ++ + R R+ E+Y+W+LG Y+EP+YS AR+I+   ++L T LDD+YD+YGT+
Sbjct: 250 HHEMEFKSKVTYTRHRITEAYLWSLGTYFEPQYSQARVITTMALILFTALDDMYDAYGTM 309

Query: 222 EELQLFTNAIEMFSSSTP 240
           EEL+LFT+A++ +    P
Sbjct: 310 EELELFTDAMDEWLPVVP 326

[Close]

BLAST of Lsi10G001840 vs. TAIR10
Match: AT2G24210.1 (AT2G24210.1 terpene synthase 10)

HSP 1 Score: 120.9 bits (302), Expect = 1.1e-27
Identity = 60/129 (46.51%), Postives = 84/129 (65.12%), Query Frame = 1


Query: 104 ALKWPIYKAMPRLTSRHYMSLYHNNPLKNNNMLLTFAKLDYNSLQKLYQKELGELSRWWK 163
           AL  P Y  M RL++R Y+ +Y       N +++ FAK+D+N +Q ++Q+EL  +S WW 
Sbjct: 231 ALDMPYYWQMRRLSTRWYIDVYGKRQNYKNLVVVEFAKIDFNIVQAIHQEELKNVSSWWM 290

Query: 164 DLKLREELPFARDRMVESYIWALGIYYEPKYSSARIISAKVIVLMTVLDDIYDSYGTLEE 223
           +  L ++L FARDR+VE+Y W +G   EP+Y   R    K+  L+T +DDIYD YGTLEE
Sbjct: 291 ETGLGKQLYFARDRIVENYFWTIGQIQEPQYGYVRQTMTKINALLTTIDDIYDIYGTLEE 350

Query: 224 LQLFTNAIE 233
           LQLFT A E
Sbjct: 351 LQLFTVAFE 359

[Close]

BLAST of Lsi10G001840 vs. NCBI nr
Match: gi|700200838|gb|KGN55971.1| (hypothetical protein Csa_3G042380 [Cucumis sativus])

HSP 1 Score: 276.2 bits (705), Expect = 5.5e-71
Identity = 146/241 (60.58%), Postives = 178/241 (73.86%), Query Frame = 1


Query: 29  MFAAAEKYSEKLGLIDSIQRLGLSYHFEDEINEI-LDQIKI--SANDDDKDEDLYIMALK 88
           MFAAAE  S++L LIDSIQRLGLS+HF++EINE+ L+QI+     ND+D DE+LY++AL+
Sbjct: 1   MFAAAENSSKRLSLIDSIQRLGLSHHFQEEINEVVLEQIEKLRKVNDEDADENLYLVALR 60

Query: 89  FRLLRQQGFYVSS-----------------------------EEAKYALKWPIYKAMPRL 148
           FRLLRQQ  ++ +                             EEAKYALKWPIYKA+PR 
Sbjct: 61  FRLLRQQASHLRTKGENILDEALAFTTTHLKAMAMDSNSPFFEEAKYALKWPIYKAVPRF 120

Query: 149 TSRHYMSLYHNNPLKNNNMLLTFAKLDYNSLQKLYQKELGELSRWWKDLKLREELPFARD 208
            +R Y+SLYHNNPLKNNN+LLTFAKLDYNSLQKLY KELGE SRWWKD KL+E LPFAR 
Sbjct: 121 MARQYISLYHNNPLKNNNVLLTFAKLDYNSLQKLYHKELGEFSRWWKDHKLKELLPFARV 180

Query: 209 RMVESYIWALGIYYEPKYSSARIISAKVIVLMTVLDDIYDSYGTLEELQLFTNAIEMFSS 238
           R+VE Y+WALGI YEPKYS+ R I  K+I  +++LDD+YD Y TL+ELQLFTNAI+ +  
Sbjct: 181 RVVEIYVWALGIQYEPKYSNTRRILVKIITFISLLDDMYDVYATLDELQLFTNAIQRWDV 240

[Close]

BLAST of Lsi10G001840 vs. NCBI nr
Match: gi|778673649|ref|XP_004151916.2| (PREDICTED: (-)-germacrene D synthase-like [Cucumis sativus])

HSP 1 Score: 232.3 bits (591), Expect = 9.2e-58
Identity = 112/157 (71.34%), Postives = 130/157 (82.80%), Query Frame = 1


Query: 76  DEDLYIMALKFRLLRQQGFYVSSEEAKYALKWPIYKAMPRLTSRHYMSLYHNNPLKNNNM 135
           DE L   + +   +        SEEAKYALKWPIYK +PR  +R+Y+SLY NNPLK +N+
Sbjct: 181 DEALEFTSTQLEAMAMDSTSPFSEEAKYALKWPIYKTLPRFMTRNYISLYRNNPLKKSNI 240

Query: 136 LLTFAKLDYNSLQKLYQKELGELSRWWKDLKLREELPFARDRMVESYIWALGIYYEPKYS 195
           LLTFAKLDYNSLQKLYQ+ELGELSRWW DLKL+E+LPFARDR+VESYIWALGIYYEPKYS
Sbjct: 241 LLTFAKLDYNSLQKLYQRELGELSRWWNDLKLKEKLPFARDRVVESYIWALGIYYEPKYS 300

Query: 196 SARIISAKVIVLMTVLDDIYDSYGTLEELQLFTNAIE 233
           SAR I AK+I ++TV+DD+YD YGTLEELQLFT AIE
Sbjct: 301 SARTIVAKIIAIITVIDDMYDCYGTLEELQLFTKAIE 337

[Close]

BLAST of Lsi10G001840 vs. NCBI nr
Match: gi|659125752|ref|XP_008462842.1| (PREDICTED: probable terpene synthase 2 isoform X1 [Cucumis melo])

HSP 1 Score: 226.5 bits (576), Expect = 5.0e-56
Identity = 110/157 (70.06%), Postives = 127/157 (80.89%), Query Frame = 1


Query: 76  DEDLYIMALKFRLLRQQGFYVSSEEAKYALKWPIYKAMPRLTSRHYMSLYHNNPLKN-NN 135
           DE L     +   +        S+EAKYALKWPIYK + R  +RHY+SLYHNNP KN NN
Sbjct: 204 DEALEFTTTQLEAMAMDSTSPFSQEAKYALKWPIYKTLSRFMTRHYISLYHNNPTKNDNN 263

Query: 136 MLLTFAKLDYNSLQKLYQKELGELSRWWKDLKLREELPFARDRMVESYIWALGIYYEPKY 195
           +LLTFAKLDYNSLQKLYQ+ELGELSRWW D KL+E+LPFARDR+VESYIWALGIYYEPKY
Sbjct: 264 ILLTFAKLDYNSLQKLYQRELGELSRWWNDSKLKEKLPFARDRVVESYIWALGIYYEPKY 323

Query: 196 SSARIISAKVIVLMTVLDDIYDSYGTLEELQLFTNAI 232
           SSAR + AK+I L+T++DD+YD YGTLEELQLFTN I
Sbjct: 324 SSARTLLAKIIALITLIDDMYDCYGTLEELQLFTNTI 360

[Close]

BLAST of Lsi10G001840 vs. NCBI nr
Match: gi|659125754|ref|XP_008462843.1| (PREDICTED: probable terpene synthase 2 isoform X2 [Cucumis melo])

HSP 1 Score: 223.4 bits (568), Expect = 4.3e-55
Identity = 135/284 (47.54%), Postives = 165/284 (58.10%), Query Frame = 1


Query: 11  EDEMVKKELEELKEGVRSMFA-------------------AAEKYSEKLG-LIDSIQRLG 70
           E++ V K+LEELKE  RSMF                     +  + +++  ++D IQ   
Sbjct: 74  EEDSVLKKLEELKEAARSMFVAAEKHSDKLCLIDSIQCLGLSYHFEDEINEILDQIQN-- 133

Query: 71  LSYHFEDEINEIL-----------------------DQIKISANDDDK------------ 130
            S H ++E  E L                          K S   D+K            
Sbjct: 134 -SAHVDEEDEEDLYIIALKFRLLRQQEIFNKFTTESGDFKESITKDEKGLLSLYEASHLR 193

Query: 131 -------DEDLYIMALKFRLLRQQGFYVSSEEAKYALKWPIYKAMPRLTSRHYMSLYHNN 190
                  DE L     +   +        S+EAKYALKWPIYK + R  +RHY+SLYHNN
Sbjct: 194 MKGESIMDEALEFTTTQLEAMAMDSTSPFSQEAKYALKWPIYKTLSRFMTRHYISLYHNN 253

Query: 191 PLKN-NNMLLTFAKLDYNSLQKLYQKELGELSRWWKDLKLREELPFARDRMVESYIWALG 232
           P KN NN+LLTFAKLDYNSLQKLYQ+ELGELSRWW D KL+E+LPFARDR+VESYIWALG
Sbjct: 254 PTKNDNNILLTFAKLDYNSLQKLYQRELGELSRWWNDSKLKEKLPFARDRVVESYIWALG 313

[Close]

BLAST of Lsi10G001840 vs. NCBI nr
Match: gi|700200835|gb|KGN55968.1| (hypothetical protein Csa_3G040870 [Cucumis sativus])

HSP 1 Score: 222.6 bits (566), Expect = 7.3e-55
Identity = 112/172 (65.12%), Postives = 134/172 (77.91%), Query Frame = 1


Query: 75  KDEDLYIMALKFRLLRQQGFYVSS-----EEAKYALKWPIYKAMPRLTSRHYMSLYHNNP 134
           K E++   AL F   + Q   + S     +E K++LKWPIYKAMPR  SRHY+SLY NNP
Sbjct: 121 KGENILDKALAFTTTQLQAIAMDSNSPFSQEVKFSLKWPIYKAMPRFMSRHYISLYQNNP 180

Query: 135 LKNNNMLLTFAKLDYNSLQKLYQKELGELSRWWKDLKLREELPFARDRMVESYIWALGIY 194
           LK+N +LLTFAKLDYNSLQKLYQKELGE SRWWKD+ LRE+L FARDR VE Y WA+G+Y
Sbjct: 181 LKDN-VLLTFAKLDYNSLQKLYQKELGEFSRWWKDMMLREQLCFARDRAVECYTWAMGVY 240

Query: 195 YEPKYSSARIISAKVIVLMTVLDDIYDSYGTLEELQLFTNAIEMFSSSTPSF 242
           YEPKYSS RI+ AKVI  +++LDD+YD+Y T EELQLFT++IE F SST SF
Sbjct: 241 YEPKYSSGRILLAKVIAFLSILDDMYDAYATFEELQLFTHSIERFCSSTISF 291

The following BLAST results are available for this feature:

BLAST of Lsi10G001840 vs. Swiss-Prot


Match Name	E-value	Identity	Description
TPSGD_VITVI	8.1e-38	48.80	(-)-germacrene D synthase OS=Vitis vinifera GN=VIT_19s0014g04930 PE=1 SV=1	[more]
GCSY1_CUCME	1.2e-36	52.86	(+)-gamma-cadinene synthase OS=Cucumis melo PE=1 SV=1	[more]
TPGAS_POGCB	2.0e-36	53.73	Germacrene A synthase OS=Pogostemon cablin PE=1 SV=1	[more]
CARS_ARTAN	1.7e-35	56.82	Beta-caryophyllene synthase OS=Artemisia annua GN=QHS1 PE=1 SV=1	[more]
5EAS_CAPAN	3.7e-35	47.10	5-epiaristolochene synthase OS=Capsicum annuum GN=EAS PE=1 SV=1	[more]

BLAST of Lsi10G001840 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L791_CUCSA	3.9e-71	60.58	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G042380 PE=3 SV=1	[more]
A0A0A0LN25_CUCSA	6.4e-58	71.34	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G427840 PE=3 SV=1	[more]
A0A0A0L2M0_CUCSA	5.1e-55	65.12	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G040870 PE=3 SV=1	[more]
A0A0B4VFP0_CUCME	5.4e-49	71.01	Sesquiterpene synthase Tps3 OS=Cucumis melo PE=2 SV=1	[more]
A0A0A0L1X6_CUCSA	2.3e-47	63.06	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G039850 PE=3 SV=1	[more]

BLAST of Lsi10G001840 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G25830.1	1.9e-29	47.01	terpene synthase-like sequence-1,8-cineole	[more]
AT3G25820.1	1.9e-29	47.01	terpene synthase-like sequence-1,8-cineole	[more]
AT4G16730.1	2.5e-29	45.52	terpene synthase 02	[more]
AT5G23960.1	7.3e-29	42.03	terpene synthase 21	[more]
AT2G24210.1	1.1e-27	46.51	terpene synthase 10	[more]

BLAST of Lsi10G001840 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700200838\|gb\|KGN55971.1\|	5.5e-71	60.58	hypothetical protein Csa_3G042380 [Cucumis sativus]	[more]
gi\|778673649\|ref\|XP_004151916.2\|	9.2e-58	71.34	PREDICTED: (-)-germacrene D synthase-like [Cucumis sativus]	[more]
gi\|659125752\|ref\|XP_008462842.1\|	5.0e-56	70.06	PREDICTED: probable terpene synthase 2 isoform X1 [Cucumis melo]	[more]
gi\|659125754\|ref\|XP_008462843.1\|	4.3e-55	47.54	PREDICTED: probable terpene synthase 2 isoform X2 [Cucumis melo]	[more]
gi\|700200835\|gb\|KGN55968.1\|	7.3e-55	65.12	hypothetical protein Csa_3G040870 [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0000287	magnesium ion binding
GO:0016829	lyase activity
GO:0010333	terpene synthase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0008152	metabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR008949	Isoprenoid_synthase_dom_sf
IPR008930	Terpenoid_cyclase/PrenylTrfase
IPR005630	Terpene_synthase_metal-bd
IPR001906	Terpene_synth_N

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008152	metabolic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0000287	magnesium ion binding
molecular_function	GO:0010333	terpene synthase activity
molecular_function	GO:0016829	lyase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi10G001840.1	Lsi10G001840.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001906	Terpene synthase, N-terminal domain	GENE3D	G3DSA:1.50.30.10		coord: 15..99 score: 3.8
IPR001906	Terpene synthase, N-terminal domain	PFAM	PF01397	Terpene_synth	coord: 10..99 score: 1.4
IPR005630	Terpene synthase, metal-binding domain	PFAM	PF03936	Terpene_synth_C	coord: 137..237 score: 8.7
IPR008930	Terpenoid cyclases/protein prenyltransferase alpha-alpha toroid	unknown	SSF48239	Terpenoid cyclases/Protein prenyltransferases	coord: 11..128 score: 7.85
IPR008949	Isoprenoid synthase domain	GENE3D	G3DSA:1.10.600.10		coord: 100..236 score: 5.5
IPR008949	Isoprenoid synthase domain	unknown	SSF48576	Terpenoid synthases	coord: 132..236 score: 1.32
None	No IPR available	unknown	Coil	Coil	coord: 5..25 scor
None	No IPR available	PANTHER	PTHR31225	FAMILY NOT NAMED	coord: 9..232 score: 3.5
None	No IPR available	PANTHER	PTHR31225:SF15	ALPHA-HUMULENE/(-)-(E)-BETA-CARYOPHYLLENE SYNTHASE	coord: 9..232 score: 3.5

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi10G001840	MELO3C024794.2	Melon (DHL92) v3.6.1	lsimedB054
Lsi10G001840	CsGy2G026440	Cucumber (Gy14) v2	cgyblsiB087
Lsi10G001840	CsaV3_2G035570	Cucumber (Chinese Long) v3	cuclsiB105

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi10G001840	Cucumber (Chinese Long) v3	cuclsiB449
Lsi10G001840	Watermelon (97103) v2	lsiwmbB054
Lsi10G001840	Wax gourd	lsiwgoB085
Lsi10G001840	Wax gourd	lsiwgoB069
Lsi10G001840	Cucumber (Gy14) v1	cgylsiB071
Lsi10G001840	Cucurbita maxima (Rimu)	cmalsiB507
Lsi10G001840	Cucurbita maxima (Rimu)	cmalsiB548
Lsi10G001840	Cucurbita moschata (Rifu)	cmolsiB491
Lsi10G001840	Cucurbita moschata (Rifu)	cmolsiB533
Lsi10G001840	Cucurbita pepo (Zucchini)	cpelsiB220
Lsi10G001840	Cucurbita pepo (Zucchini)	cpelsiB589
Lsi10G001840	Wild cucumber (PI 183967)	cpilsiB092
Lsi10G001840	Cucumber (Chinese Long) v2	culsiB094
Lsi10G001840	Melon (DHL92) v3.5.1	lsimeB053
Lsi10G001840	Watermelon (Charleston Gray)	lsiwcgB055
Lsi10G001840	Watermelon (97103) v1	lsiwmB068
Lsi10G001840	Silver-seed gourd	carlsiB374
Lsi10G001840	Silver-seed gourd	carlsiB870