Lsi09G017150 (gene) Bottle gourd (USVL1VR-Ls)

Overview
NameLsi09G017150
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionPyruvate dehydrogenase E1 component subunit alpha
Locationchr09 : 25701175 .. 25703412 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTTGTTGGGTTGATTGATTTGTTAATGGGTTTCTAATGTTATGGAAATCATGGGAGAATCGGGTAATGTAGCATGAAAATTTCGCTTTGTATGCCCGCATGGAATCATCTAGCTTTAACGATTTTGCTTTTGTTGGTTTTTTAATTTGTTGATTTTGGTCTCCCTGTTGTTGTTTTCTTTTGGAAATTTAGATGGTTTGAGATTGTCATTATGAAATTGGAGGTTATTATTTGTTCTTTTGCCCCACCAGTGGAATTAGCTTTGTTGAATTATTGAATTCGTGCAAAATTTGACTCTACTGGAAGCTTCTGTATGGCTTTGCTACATGAACTATTTTGTTTAAAAGTTCTTCCCTTTTTTTTTTGGTTCCCTTTATTGTCTCCACACAGCTGATTACCAAAGAAGAGGGTTTGGTACTCTACGAGGACATGATATTAGGCAGAGAGTTTGAGGATATGTGTGCACAGATGTATTACAGAGGCAAAATGTTTGGTTTTGTTCACCTTTACAATGGCCAAGAAGCTGTTTCCACCGGGTTCATCAAGCTTCTTACAAAGAGGGATACTGTAGTAAGCACTTATCGTGATCACGTTCATGCTCTCAGCAAGGGAGTTCCATCCCGTGAGGTCATGAGTGAGCTTTTTGGAAAGACCACTGGTTGCTGCCGTGGCCAAGGTGGCTCGATGCATATGTTCTCAAAAGAACATAATCTGATAGGTGGCTTTGCCTTCATCGGGGAAGGCATACCAGTGGCCACTGGTGCTGCATTCACATCTAAATACAAGAGAGAAGTTTTAAAAGAGGCAGACTGTGAAGATGTCACACTGGCGTTTTTCGGAGATGGGACTTGCAACAATGGCCAGTTCTTTGAGTGTTTGAACATGGCAGCTCTGTGGAAATTGCCAATTGTATTTGTTGTGGAGAATAATCTTTGGGCAATTGGAATGTCACATTTGAGGGCTACTTCAGATCCTGAGATTTGGAAGAAGGGTCCTGCATTTGGAATGCCTGGCGTTCATGTTGATGGTATGGATGTTTTGAAGGTGAGGGAGGTTGCAAAGGAGGCAATTGGAAGAGCCAGGAGAGGAGAAGGACCGACTCTCGTGGAATGCGAAACCTATAGGTTTAGAGGACACTCACTGGCTGATCCAGATGAACTCCGCGACCCTGGTTAGTGCTCTTAAATTCATACCTACATTTGGTTCTGTCATTTCTAAATATGATTTTGCTTGGCCTTGTTGACATTTATTATTACGGCTAGTCAAAGTTCCTTAATTCATTTCTCATGAATCATGATAGCCAATCTCTTAATCCGTACTCCATCATAGCATTTAAGGATAATTCATTCAGGGTTGTAATGAGCTGCACCACTATAAATTCACATAGTTATCTCATTTGTTCATTTATCATTTTTTATGAACAAAGAGGGAAATGAGTGTTATAGTTGATTTTACCAGTATTTAATCCAGAGTGCATTATCTGAGGCCAGATTACATTGTTCAATCTTCAAATATCAGTCTACTGGATACAATTAGTGGACTCATCTTGCAATGTTTACTCTATAGATTCTAAGTGAACCAAACATCATAGAAGTGGAAGTGACAACTGGTTTTTTCTTTTATTTCAAGTGAACATTGTAAAAGGAAGAAACCAGTCAAACTTGATGGACATAACTACTCCATTATGTGACTTTTATATGATTGCTCACAAGGAAAAACACTTGGAACTCATGTGATATATATTTAGCAAATTGGCATGATAATAATATGTAGATTTCTAATGTGGTGTCTTTAGAGTTCATGGACAGACTCGATTAGATGATAGAACTCAATGTCCCCATCAGGTTCTTCATTGTACTTTGCTTACTTACACACACAAATGTAGTAGTTTCTTATCCAAGTATAATAACTCTAGAAAGTACAAAGGATTAAGAATTCCCGATTTAACCGTGGCAGACGAGAAGGCCCGTTATGCTGCTAGAGATCCCATTGCAGCTTTGAAGAAATACTTGCTGGAGAATAATCTAGCCAGTGAACAGGAGCTAAAAGGGATAAAGGACAAGATAGTTGAGGTGGTTGAGGATGCAGTTAGTTTTGCAGATGAAAGTCCTCTTCCAGCCAGAAGCCAGTTATTAGAGAATGTGTTTGCTGATCCTAAAGGCTTTGGAATTGGGCCTGATGGAAAGTACAGATGTGAGGATCCCAAGTTCACAGAAGGCACTGCACATGTCTAA

mRNA sequence

ATGGATTTATGGTTTGAGATTGTCATTATGAAATTGGAGCTGATTACCAAAGAAGAGGGTTTGGTACTCTACGAGGACATGATATTAGGCAGAGAGTTTGAGGATATGTGTGCACAGATGTATTACAGAGGCAAAATGTTTGGTTTTGTTCACCTTTACAATGGCCAAGAAGCTGTTTCCACCGGGTTCATCAAGCTTCTTACAAAGAGGGATACTGTAGTAAGCACTTATCGTGATCACGTTCATGCTCTCAGCAAGGGAGTTCCATCCCGTGAGGTCATGAGTGAGCTTTTTGGAAAGACCACTGGTTGCTGCCGTGGCCAAGGTGGCTCGATGCATATGTTCTCAAAAGAACATAATCTGATAGGTGGCTTTGCCTTCATCGGGGAAGGCATACCAGTGGCCACTGGTGCTGCATTCACATCTAAATACAAGAGAGAAGTTTTAAAAGAGGCAGACTGTGAAGATGTCACACTGGCGTTTTTCGGAGATGGGACTTGCAACAATGGCCAGTTCTTTGAGTGTTTGAACATGGCAGCTCTGTGGAAATTGCCAATTGTATTTGTTGTGGAGAATAATCTTTGGGCAATTGGAATGTCACATTTGAGGGCTACTTCAGATCCTGAGATTTGGAAGAAGGGTCCTGCATTTGGAATGCCTGGCGTTCATGTTGATGGTATGGATGTTTTGAAGGTGAGGGAGGTTGCAAAGGAGGCAATTGGAAGAGCCAGGAGAGGAGAAGGACCGACTCTCGTGGAATGCGAAACCTATAGGTTTAGAGGACACTCACTGGCTGATCCAGATGAACTCCGCGACCCTGTGAACATTGTAAAAGGAAGAAACCAGTCAAACTTGATGGACATAACTACTCCATTATGTGACTTTTATATGATTGCTCACAAGGAAAAACACTTGGAACTCATAAAGTACAAAGGATTAAGAATTCCCGATTTAACCGTGGCAGACGAGAAGGCCCGTTATGCTGCTAGAGATCCCATTGCAGCTTTGAAGAAATACTTGCTGGAGAATAATCTAGCCAGTGAACAGGAGCTAAAAGGGATAAAGGACAAGATAGTTGAGGTGGTTGAGGATGCAGTTAGTTTTGCAGATGAAAGTCCTCTTCCAGCCAGAAGCCAGTTATTAGAGAATGTGTTTGCTGATCCTAAAGGCTTTGGAATTGGGCCTGATGGAAAGTACAGATGTGAGGATCCCAAGTTCACAGAAGGCACTGCACATGTCTAA

Coding sequence (CDS)

ATGGATTTATGGTTTGAGATTGTCATTATGAAATTGGAGCTGATTACCAAAGAAGAGGGTTTGGTACTCTACGAGGACATGATATTAGGCAGAGAGTTTGAGGATATGTGTGCACAGATGTATTACAGAGGCAAAATGTTTGGTTTTGTTCACCTTTACAATGGCCAAGAAGCTGTTTCCACCGGGTTCATCAAGCTTCTTACAAAGAGGGATACTGTAGTAAGCACTTATCGTGATCACGTTCATGCTCTCAGCAAGGGAGTTCCATCCCGTGAGGTCATGAGTGAGCTTTTTGGAAAGACCACTGGTTGCTGCCGTGGCCAAGGTGGCTCGATGCATATGTTCTCAAAAGAACATAATCTGATAGGTGGCTTTGCCTTCATCGGGGAAGGCATACCAGTGGCCACTGGTGCTGCATTCACATCTAAATACAAGAGAGAAGTTTTAAAAGAGGCAGACTGTGAAGATGTCACACTGGCGTTTTTCGGAGATGGGACTTGCAACAATGGCCAGTTCTTTGAGTGTTTGAACATGGCAGCTCTGTGGAAATTGCCAATTGTATTTGTTGTGGAGAATAATCTTTGGGCAATTGGAATGTCACATTTGAGGGCTACTTCAGATCCTGAGATTTGGAAGAAGGGTCCTGCATTTGGAATGCCTGGCGTTCATGTTGATGGTATGGATGTTTTGAAGGTGAGGGAGGTTGCAAAGGAGGCAATTGGAAGAGCCAGGAGAGGAGAAGGACCGACTCTCGTGGAATGCGAAACCTATAGGTTTAGAGGACACTCACTGGCTGATCCAGATGAACTCCGCGACCCTGTGAACATTGTAAAAGGAAGAAACCAGTCAAACTTGATGGACATAACTACTCCATTATGTGACTTTTATATGATTGCTCACAAGGAAAAACACTTGGAACTCATAAAGTACAAAGGATTAAGAATTCCCGATTTAACCGTGGCAGACGAGAAGGCCCGTTATGCTGCTAGAGATCCCATTGCAGCTTTGAAGAAATACTTGCTGGAGAATAATCTAGCCAGTGAACAGGAGCTAAAAGGGATAAAGGACAAGATAGTTGAGGTGGTTGAGGATGCAGTTAGTTTTGCAGATGAAAGTCCTCTTCCAGCCAGAAGCCAGTTATTAGAGAATGTGTTTGCTGATCCTAAAGGCTTTGGAATTGGGCCTGATGGAAAGTACAGATGTGAGGATCCCAAGTTCACAGAAGGCACTGCACATGTCTAA

Protein sequence

MDLWFEIVIMKLELITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSKEHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLNMAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPVNIVKGRNQSNLMDITTPLCDFYMIAHKEKHLELIKYKGLRIPDLTVADEKARYAARDPIAALKKYLLENNLASEQELKGIKDKIVEVVEDAVSFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPKFTEGTAHV
Homology
[Close]
BLAST of Lsi09G017150 vs. Swiss-Prot
Match: ODPA3_ARATH (Pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic OS=Arabidopsis thaliana GN=PDH-E1 ALPHA PE=2 SV=1)

HSP 1 Score: 506.9 bits (1304), Expect = 2.1e-142
Identity = 256/333 (76.88%), Postives = 279/333 (83.78%), Query Frame = 1 

Query: 14  LITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLTKRDTV 73
           LITKEEGL LYEDMILGR FEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLTK D+V
Sbjct: 78  LITKEEGLELYEDMILGRSFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLTKSDSV 137

Query: 74  VSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSKEHNLIGGFAFIGEGIP 133
           VSTYRDHVHALSKGV +R VMSELFGK TGCCRGQGGSMHMFSKEHN++GGFAFIGEGIP
Sbjct: 138 VSTYRDHVHALSKGVSARAVMSELFGKVTGCCRGQGGSMHMFSKEHNMLGGFAFIGEGIP 197

Query: 134 VATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLNMAALWKLPIVFVVENN 193
           VATGAAF+SKY+REVLK+ DC+DVT+AFFGDGTCNNGQFFECLNMAAL+KLPI+FVVENN
Sbjct: 198 VATGAAFSSKYRREVLKQ-DCDDVTVAFFGDGTCNNGQFFECLNMAALYKLPIIFVVENN 257

Query: 194 LWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGEGPTLVE 253
           LWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEA+ RARRGEGPTLVE
Sbjct: 258 LWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAVTRARRGEGPTLVE 317

Query: 254 CETYRFRGHSLADPDELRDPVNIVKGRNQSNLMDITTPLCDFYMIAHKEKHLELIKYKGL 313
           CETYRFRGHSLADPDELRD     K   +  +  +   L +  +   KE  L+ I+ K  
Sbjct: 318 CETYRFRGHSLADPDELRDAAEKAKYAARDPIAALKKYLIENKLA--KEAELKSIEKK-- 377

Query: 314 RIPDLTVADEKARYAARDPIAALKKYLLENNLA 347
            I +L   +E   +A   P    +  LLEN  A
Sbjct: 378 -IDEL--VEEAVEFADASPQPG-RSQLLENVFA 401

[Close]
BLAST of Lsi09G017150 vs. Swiss-Prot
Match: ODPA3_ORYSJ (Pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic OS=Oryza sativa subsp. japonica GN=Os04g0119400 PE=2 SV=2)

HSP 1 Score: 483.0 bits (1242), Expect = 3.2e-135
Identity = 230/261 (88.12%), Postives = 243/261 (93.10%), Query Frame = 1 

Query: 15  ITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLTKRDTVV 74
           +T+EE L LYEDM+LGR FEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLL + D VV
Sbjct: 73  VTREEALELYEDMVLGRIFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLNQADCVV 132

Query: 75  STYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSKEHNLIGGFAFIGEGIPV 134
           STYRDHVHALSKGVP+R VM+ELFGK TGCCRGQGGSMHMFS+ HNL+GGFAFIGEGIPV
Sbjct: 133 STYRDHVHALSKGVPARSVMAELFGKATGCCRGQGGSMHMFSEPHNLLGGFAFIGEGIPV 192

Query: 135 ATGAAFTSKYKREVLKEA--DCEDVTLAFFGDGTCNNGQFFECLNMAALWKLPIVFVVEN 194
           ATGAAF +KY+ EVLK++  D  DVTLAFFGDGTCNNGQFFECLNMA LWKLPIVFVVEN
Sbjct: 193 ATGAAFAAKYRHEVLKQSSPDGLDVTLAFFGDGTCNNGQFFECLNMAQLWKLPIVFVVEN 252

Query: 195 NLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGEGPTLV 254
           NLWAIGMSHLRATSDPEI+KKGPAFGMPGVHVDGMDVLKVREVAKEAI RARRGEGPTLV
Sbjct: 253 NLWAIGMSHLRATSDPEIYKKGPAFGMPGVHVDGMDVLKVREVAKEAIERARRGEGPTLV 312

Query: 255 ECETYRFRGHSLADPDELRDP 274
           ECETYRFRGHSLADPDELR P
Sbjct: 313 ECETYRFRGHSLADPDELRRP 333

[Close]
BLAST of Lsi09G017150 vs. Swiss-Prot
Match: ODPA_PORPU (Pyruvate dehydrogenase E1 component subunit alpha OS=Porphyra purpurea GN=pdhA PE=3 SV=1)

HSP 1 Score: 412.9 bits (1060), Expect = 4.1e-114
Identity = 211/341 (61.88%), Postives = 248/341 (72.73%), Query Frame = 1 

Query: 15  ITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLTKRDTVV 74
           +TK + LVLYEDM+LGR FEDMCAQMYY+GKMFGFVHLYNGQEAVSTG IKLL  +D V 
Sbjct: 19  LTKHKLLVLYEDMLLGRNFEDMCAQMYYKGKMFGFVHLYNGQEAVSTGVIKLLDSKDYVC 78

Query: 75  STYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSKEHNLIGGFAFIGEGIPV 134
           STYRDHVHALSKGVPS+ VM+ELFGK TGC RG+GGSMH+FS  HN +GGFAFI EGIPV
Sbjct: 79  STYRDHVHALSKGVPSQNVMAELFGKETGCSRGRGGSMHIFSAPHNFLGGFAFIAEGIPV 138

Query: 135 ATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLNMAALWKLPIVFVVENNL 194
           ATGAAF S Y+++VLKE     VT  FFGDGT NNGQFFECLNMA LWKLPI+FVVENN 
Sbjct: 139 ATGAAFQSIYRQQVLKEPGELRVTACFFGDGTTNNGQFFECLNMAVLWKLPIIFVVENNQ 198

Query: 195 WAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGEGPTLVEC 254
           WAIGM+H R++S PEI KK  AFG+PG+ VDGMDVL VR+VA++A+ RAR+G+GPTL+E 
Sbjct: 199 WAIGMAHHRSSSIPEIHKKAEAFGLPGIEVDGMDVLAVRQVAEKAVERARQGQGPTLIEA 258

Query: 255 ETYRFRGHSLADPDEL-----------RDPVNIVKGRNQSNLMDITTPLCDFYMIAHKEK 314
            TYRFRGHSLADPDEL           RDP+  +K     N +  +  L D         
Sbjct: 259 LTYRFRGHSLADPDELRSRQEKEAWVARDPIKKLKKHILDNQIASSDELNDI-------- 318

Query: 315 HLELIKYKGLRIPDLTVADEKARYAARDPIAALKKYLLENN 345
                    ++I DL  + E A  +    I+ LK+YL  +N
Sbjct: 319 ------QSSVKI-DLEQSVEFAMSSPEPNISELKRYLFADN 344

[Close]
BLAST of Lsi09G017150 vs. Swiss-Prot
Match: ODPA_PYRYE (Pyruvate dehydrogenase E1 component subunit alpha OS=Pyropia yezoensis GN=pdhA PE=3 SV=1)

HSP 1 Score: 408.7 bits (1049), Expect = 7.7e-113
Identity = 192/257 (74.71%), Postives = 217/257 (84.44%), Query Frame = 1 

Query: 15  ITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLTKRDTVV 74
           + K   LVLYEDM+LGR FEDMCAQMYY+GKMFGFVHLYNGQEAVSTG IKLL   D V 
Sbjct: 21  LNKSNLLVLYEDMLLGRNFEDMCAQMYYKGKMFGFVHLYNGQEAVSTGVIKLLNPTDYVC 80

Query: 75  STYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSKEHNLIGGFAFIGEGIPV 134
           STYRDHVHALSKGVPS+ VM+ELFGK TGC +G+GGSMH+FS  HN +GGFAFI EGIPV
Sbjct: 81  STYRDHVHALSKGVPSKNVMAELFGKETGCSKGRGGSMHIFSAPHNFLGGFAFIAEGIPV 140

Query: 135 ATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLNMAALWKLPIVFVVENNL 194
           ATGAAF S Y+++VLKE +   VT  FFGDGT NNGQFFECLNMA LWKLPI+FVVENN 
Sbjct: 141 ATGAAFQSIYRQQVLKETEDLRVTACFFGDGTTNNGQFFECLNMAVLWKLPIIFVVENNQ 200

Query: 195 WAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGEGPTLVEC 254
           WAIGM+H R++S PEI KK  AFG+PG+ VDGMDVL VR+ AK+A+ RAR+G+GPTL+E 
Sbjct: 201 WAIGMAHHRSSSIPEIHKKAEAFGLPGIEVDGMDVLAVRQAAKQAVQRARQGDGPTLIEA 260

Query: 255 ETYRFRGHSLADPDELR 272
            TYRFRGHSLADPDELR
Sbjct: 261 LTYRFRGHSLADPDELR 277

[Close]
BLAST of Lsi09G017150 vs. Swiss-Prot
Match: ODPA_RHIME (Pyruvate dehydrogenase E1 component subunit alpha OS=Rhizobium meliloti (strain 1021) GN=pdhA PE=3 SV=1)

HSP 1 Score: 252.7 bits (644), Expect = 7.0e-66
Identity = 120/267 (44.94%), Postives = 174/267 (65.17%), Query Frame = 1 

Query: 16  TKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLTKRDTVVS 75
           +KE+ L  Y +M+L R FE+   Q+Y  G + GF HLY GQEAV  G    L + D V++
Sbjct: 32  SKEDDLKAYREMLLIRRFEEKAGQLYGMGFIGGFCHLYIGQEAVVVGMQLALKEGDQVIT 91

Query: 76  TYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSKEHNLIGGFAFIGEGIPVA 135
            YRDH H L+ G+ +R VM+EL G+  G  +G+GGSMHMFSKE +  GG   +G  + + 
Sbjct: 92  GYRDHGHMLACGMSARGVMAELTGRRGGLSKGKGGSMHMFSKEKHFYGGHGIVGAQVSLG 151

Query: 136 TGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLNMAALWKLPIVFVVENNLW 195
           TG AF ++Y+         ++V+LA+FGDG  N GQ +E  NMAALWKLP++++VENN +
Sbjct: 152 TGLAFANRYRGN-------DNVSLAYFGDGAANQGQVYESFNMAALWKLPVIYIVENNRY 211

Query: 196 AIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGEGPTLVECE 255
           A+G S  RA++  +  ++G +FG+PG  VDGMDV  V+  A EA+   R G+GP ++E  
Sbjct: 212 AMGTSVSRASAQTDFSQRGASFGIPGYQVDGMDVRAVKAAADEAVEHCRSGKGPIILEML 271

Query: 256 TYRFRGHSLADPDELRDPVNIVKGRNQ 283
           TYR+RGHS++DP + R    + K R++
Sbjct: 272 TYRYRGHSMSDPAKYRSKDEVQKMRSE 291

[Close]
BLAST of Lsi09G017150 vs. TrEMBL
Match: A0A0A0KFF3_CUCSA (Pyruvate dehydrogenase E1 component subunit alpha OS=Cucumis sativus GN=Csa_6G095870 PE=4 SV=1)

HSP 1 Score: 548.1 bits (1411), Expect = 9.0e-153
Identity = 276/336 (82.14%), Postives = 291/336 (86.61%), Query Frame = 1 

Query: 14  LITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLTKRDTV 73
           LITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLT+RDTV
Sbjct: 77  LITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLTQRDTV 136

Query: 74  VSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSKEHNLIGGFAFIGEGIP 133
           VSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSKEHNLIGGFAFIGEGIP
Sbjct: 137 VSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSKEHNLIGGFAFIGEGIP 196

Query: 134 VATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLNMAALWKLPIVFVVENN 193
           VATGAAFTSKYKREVLKEADC+DVTLAFFGDGTCNNGQFFECLNMAALWKLPIVFVVENN
Sbjct: 197 VATGAAFTSKYKREVLKEADCQDVTLAFFGDGTCNNGQFFECLNMAALWKLPIVFVVENN 256

Query: 194 LWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGEGPTLVE 253
           LWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGEGPTLVE
Sbjct: 257 LWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGEGPTLVE 316

Query: 254 CETYRFRGHSLADPDELRDPVNIVKGRNQSNLMDITTPLCDFYMIAHK---EKHLELIKY 313
           CETYRFRGHSLADPDELRDP    +   +  +  +       YM+ +K   E+ L+ IK 
Sbjct: 317 CETYRFRGHSLADPDELRDPDEKARYAARDPIAALKK-----YMLENKLANEQELKAIKD 376

Query: 314 KGLRIPDLTVADEKARYAARDPIAALKKYLLENNLA 347
           K      + V +E  ++A   P  A +  LLEN  A
Sbjct: 377 K-----IVEVVEEAVQFADESPHPA-RSQLLENVFA 401

[Close]
BLAST of Lsi09G017150 vs. TrEMBL
Match: M1B234_SOLTU (Pyruvate dehydrogenase E1 component subunit alpha OS=Solanum tuberosum GN=PGSC0003DMG402013561 PE=4 SV=1)

HSP 1 Score: 528.5 bits (1360), Expect = 7.4e-147
Identity = 261/333 (78.38%), Postives = 283/333 (84.98%), Query Frame = 1 

Query: 14  LITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLTKRDTV 73
           LITKEEGLVLYEDM+LGR FEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLL K D+V
Sbjct: 86  LITKEEGLVLYEDMVLGRTFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLKKEDSV 145

Query: 74  VSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSKEHNLIGGFAFIGEGIP 133
           VSTYRDHVHALSKGVP+R+VMSELFGKTTGCCRGQGGSMHMFSKEHN++GGFAFIGEGIP
Sbjct: 146 VSTYRDHVHALSKGVPARQVMSELFGKTTGCCRGQGGSMHMFSKEHNVLGGFAFIGEGIP 205

Query: 134 VATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLNMAALWKLPIVFVVENN 193
           VATGAAFTSKY+REVLKEADC+ VTLAFFGDGTCNNGQF+ECLNMAALWKLPI+FVVENN
Sbjct: 206 VATGAAFTSKYRREVLKEADCDHVTLAFFGDGTCNNGQFYECLNMAALWKLPIIFVVENN 265

Query: 194 LWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGEGPTLVE 253
           LWAIGMSHLR+TSDPEIWKKGPAFGMPGVHVDGMDVLKVREVA EA+GRARRGEGPTLVE
Sbjct: 266 LWAIGMSHLRSTSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAMEAVGRARRGEGPTLVE 325

Query: 254 CETYRFRGHSLADPDELRDPVNIVKGRNQSNLMDITTPLCDFYMIAHKEKHLELIKYKGL 313
           CETYRFRGHSLADPDELRDP      +N     D  T L   YM  +   +   +K    
Sbjct: 326 CETYRFRGHSLADPDELRDPAE----KNHYATRDPITAL-KKYMFENNLVNEAELKAIDK 385

Query: 314 RIPDLTVADEKARYAARDPIAALKKYLLENNLA 347
           +I +L   +E   +A   P+ A +  LLEN  A
Sbjct: 386 KIDEL--VEESVEFAEASPVPA-RNQLLENVFA 410

[Close]
BLAST of Lsi09G017150 vs. TrEMBL
Match: B5LAW2_CAPAN (Pyruvate dehydrogenase E1 component subunit alpha OS=Capsicum annuum PE=2 SV=1)

HSP 1 Score: 526.2 bits (1354), Expect = 3.7e-146
Identity = 260/333 (78.08%), Postives = 282/333 (84.68%), Query Frame = 1 

Query: 14  LITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLTKRDTV 73
           LITKEEGL LYEDM+LGR FEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLL K D+V
Sbjct: 80  LITKEEGLELYEDMVLGRAFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLKKEDSV 139

Query: 74  VSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSKEHNLIGGFAFIGEGIP 133
           VSTYRDHVHALSKGVP+R+VMSELFGKTTGCCRGQGGSMHMFSKEHN++GGFAFIGEGIP
Sbjct: 140 VSTYRDHVHALSKGVPARQVMSELFGKTTGCCRGQGGSMHMFSKEHNVLGGFAFIGEGIP 199

Query: 134 VATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLNMAALWKLPIVFVVENN 193
           VATGAAFTSKY+REVLKEADC+ VTLAFFGDGTCNNGQF+ECLNMAALWKLPI+FVVENN
Sbjct: 200 VATGAAFTSKYRREVLKEADCDHVTLAFFGDGTCNNGQFYECLNMAALWKLPIIFVVENN 259

Query: 194 LWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGEGPTLVE 253
           LWAIGMSHLR+TSDPEIWKKGPAFGMPGVHVDGMDVLKVREVA EA+GRARRGEGPTLVE
Sbjct: 260 LWAIGMSHLRSTSDPEIWKKGPAFGMPGVHVDGMDVLKVREVANEAVGRARRGEGPTLVE 319

Query: 254 CETYRFRGHSLADPDELRDPVNIVKGRNQSNLMDITTPLCDFYMIAHKEKHLELIKYKGL 313
           CETYRFRGHSLADPDELRDP      +N     D  T L   YM  +   +   +K    
Sbjct: 320 CETYRFRGHSLADPDELRDPAE----KNHYATRDPITAL-KKYMFENNLVNEAELKAIDK 379

Query: 314 RIPDLTVADEKARYAARDPIAALKKYLLENNLA 347
           +I +L   +E   +A   P+ A +  LLEN  A
Sbjct: 380 KIDEL--VEESVEFADASPVPA-RNQLLENVFA 404

[Close]
BLAST of Lsi09G017150 vs. TrEMBL
Match: A0A068UJA1_COFCA (Pyruvate dehydrogenase E1 component subunit alpha OS=Coffea canephora GN=GSCOC_T00027639001 PE=4 SV=1)

HSP 1 Score: 523.5 bits (1347), Expect = 2.4e-145
Identity = 258/333 (77.48%), Postives = 279/333 (83.78%), Query Frame = 1 

Query: 14  LITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLTKRDTV 73
           LITKEEGL LYEDM+LGR FEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIK L + D V
Sbjct: 93  LITKEEGLELYEDMVLGRSFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKFLKQEDCV 152

Query: 74  VSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSKEHNLIGGFAFIGEGIP 133
           VSTYRDHVHALSKGVP+R VMSELFGKTTGCCRGQGGSMHMFSKEHNL+GGFAFIGEGIP
Sbjct: 153 VSTYRDHVHALSKGVPARAVMSELFGKTTGCCRGQGGSMHMFSKEHNLLGGFAFIGEGIP 212

Query: 134 VATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLNMAALWKLPIVFVVENN 193
           VATGAAFTSKY+REVLKEADC+ VTLAFFGDGTCNNGQFFECLNMAALWKLPIVFVVENN
Sbjct: 213 VATGAAFTSKYRREVLKEADCDHVTLAFFGDGTCNNGQFFECLNMAALWKLPIVFVVENN 272

Query: 194 LWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGEGPTLVE 253
           LWAIGMSHLRATSDP+IWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGEGPTLVE
Sbjct: 273 LWAIGMSHLRATSDPQIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGEGPTLVE 332

Query: 254 CETYRFRGHSLADPDELRDPVNIVKGRNQSNLMDITTPLCDFYMIAHKEKHLELIKYKGL 313
           CETYRFRGHSLADPDELRDP    +  ++  +  +       YM  +       +K    
Sbjct: 333 CETYRFRGHSLADPDELRDPAEKARYASRDPISALKK-----YMFENNLASEAELKAIDK 392

Query: 314 RIPDLTVADEKARYAARDPIAALKKYLLENNLA 347
           +I +L   ++   +A   P+ A +  LLEN  A
Sbjct: 393 KIDEL--VEDAVEFADESPLPA-RSQLLENVFA 417

[Close]
BLAST of Lsi09G017150 vs. TrEMBL
Match: A9PF50_POPTR (Pyruvate dehydrogenase E1 component subunit alpha OS=Populus trichocarpa GN=POPTR_0002s18020g PE=2 SV=1)

HSP 1 Score: 521.9 bits (1343), Expect = 6.9e-145
Identity = 246/260 (94.62%), Postives = 254/260 (97.69%), Query Frame = 1 

Query: 14  LITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLTKRDTV 73
           LITKEEGL +YEDMILGR FEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLL + D+V
Sbjct: 91  LITKEEGLEVYEDMILGRAFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLKREDSV 150

Query: 74  VSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSKEHNLIGGFAFIGEGIP 133
           VSTYRDHVHALSKGVP+R VMSELFGKTTGCCRGQGGSMHMFSKEHNLIGGFAFIGEGIP
Sbjct: 151 VSTYRDHVHALSKGVPARAVMSELFGKTTGCCRGQGGSMHMFSKEHNLIGGFAFIGEGIP 210

Query: 134 VATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLNMAALWKLPIVFVVENN 193
           VATGAAF+SKY+REVLKEADC+ VTLAFFGDGTCNNGQFFECLNMAALWKLPIVFVVENN
Sbjct: 211 VATGAAFSSKYRREVLKEADCDHVTLAFFGDGTCNNGQFFECLNMAALWKLPIVFVVENN 270

Query: 194 LWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGEGPTLVE 253
           LWAIGMSH+RATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGEGPTLVE
Sbjct: 271 LWAIGMSHVRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGEGPTLVE 330

Query: 254 CETYRFRGHSLADPDELRDP 274
           CETYRFRGHSLADPDELRDP
Sbjct: 331 CETYRFRGHSLADPDELRDP 350

[Close]
BLAST of Lsi09G017150 vs. TAIR10
Match: AT1G01090.1 (AT1G01090.1 pyruvate dehydrogenase E1 alpha)

HSP 1 Score: 506.9 bits (1304), Expect = 1.2e-143
Identity = 256/333 (76.88%), Postives = 279/333 (83.78%), Query Frame = 1 

Query: 14  LITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLTKRDTV 73
           LITKEEGL LYEDMILGR FEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLTK D+V
Sbjct: 78  LITKEEGLELYEDMILGRSFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLTKSDSV 137

Query: 74  VSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSKEHNLIGGFAFIGEGIP 133
           VSTYRDHVHALSKGV +R VMSELFGK TGCCRGQGGSMHMFSKEHN++GGFAFIGEGIP
Sbjct: 138 VSTYRDHVHALSKGVSARAVMSELFGKVTGCCRGQGGSMHMFSKEHNMLGGFAFIGEGIP 197

Query: 134 VATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLNMAALWKLPIVFVVENN 193
           VATGAAF+SKY+REVLK+ DC+DVT+AFFGDGTCNNGQFFECLNMAAL+KLPI+FVVENN
Sbjct: 198 VATGAAFSSKYRREVLKQ-DCDDVTVAFFGDGTCNNGQFFECLNMAALYKLPIIFVVENN 257

Query: 194 LWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGEGPTLVE 253
           LWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEA+ RARRGEGPTLVE
Sbjct: 258 LWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAVTRARRGEGPTLVE 317

Query: 254 CETYRFRGHSLADPDELRDPVNIVKGRNQSNLMDITTPLCDFYMIAHKEKHLELIKYKGL 313
           CETYRFRGHSLADPDELRD     K   +  +  +   L +  +   KE  L+ I+ K  
Sbjct: 318 CETYRFRGHSLADPDELRDAAEKAKYAARDPIAALKKYLIENKLA--KEAELKSIEKK-- 377

Query: 314 RIPDLTVADEKARYAARDPIAALKKYLLENNLA 347
            I +L   +E   +A   P    +  LLEN  A
Sbjct: 378 -IDEL--VEEAVEFADASPQPG-RSQLLENVFA 401

[Close]
BLAST of Lsi09G017150 vs. TAIR10
Match: AT1G59900.1 (AT1G59900.1 pyruvate dehydrogenase complex E1 alpha subunit)

HSP 1 Score: 218.0 bits (554), Expect = 1.1e-56
Identity = 120/316 (37.97%), Postives = 171/316 (54.11%), Query Frame = 1 

Query: 16  TKEEGLVLYEDMILGREFEDMCAQMYYRGKMF-GFVHLYNGQEAVSTGFIKLLTKRDTVV 75
           + +E L  +  M L R  E + A   Y+ K+  GF HLY+GQEAV+ G    +TK+D ++
Sbjct: 55  SSQELLDFFRTMALMRRME-IAADSLYKAKLIRGFCHLYDGQEAVAIGMEAAITKKDAII 114

Query: 76  STYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSKEHNLIGGFAFIGEGIPV 135
           + YRDH   L +G    EV SEL G+  GC +G+GGSMH + KE +  GG   +G  +P+
Sbjct: 115 TAYRDHCIFLGRGGSLHEVFSELMGRQAGCSKGKGGSMHFYKKESSFYGGHGIVGAQVPL 174

Query: 136 ATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLNMAALWKLPIVFVVENNL 195
             G AF  KY +E       E VT A +GDG  N GQ FE LN++ALW LP + V ENN 
Sbjct: 175 GCGIAFAQKYNKE-------EAVTFALYGDGAANQGQLFEALNISALWDLPAILVCENNH 234

Query: 196 WAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGEGPTLVEC 255
           + +G +  RA   P  +K+G    +PG+ VDGMD   V++  K A   A   +GP ++E 
Sbjct: 235 YGMGTAEWRAAKSPSYYKRGDY--VPGLKVDGMDAFAVKQACKFAKQHALE-KGPIILEM 294

Query: 256 ETYRFRGHSLADP-------DEL------RDPVNIVKGRNQSNLMDITTPLCDFYMIAHK 315
           +TYR+ GHS++DP       DE+      RDP+  +K    S+ +     L D      K
Sbjct: 295 DTYRYHGHSMSDPGSTYRTRDEISGVRQERDPIERIKKLVLSHDLATEKELKDMEKEIRK 354

Query: 316 EKHLELIKYKGLRIPD 318
           E    + K K   +P+
Sbjct: 355 EVDDAIAKAKDCPMPE 359

[Close]
BLAST of Lsi09G017150 vs. TAIR10
Match: AT1G24180.1 (AT1G24180.1 Thiamin diphosphate-binding fold (THDP-binding) superfamily protein)

HSP 1 Score: 211.8 bits (538), Expect = 7.8e-55
Identity = 115/316 (36.39%), Postives = 171/316 (54.11%), Query Frame = 1 

Query: 16  TKEEGLVLYEDMILGREFEDMCAQMYYRGKMF-GFVHLYNGQEAVSTGFIKLLTKRDTVV 75
           + EE L  + DM   R  E + A   Y+ K+  GF HLY+GQEA++ G    +TK+D ++
Sbjct: 59  SSEEILAFFRDMARMRRME-IAADSLYKAKLIRGFCHLYDGQEALAVGMEAAITKKDAII 118

Query: 76  STYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSKEHNLIGGFAFIGEGIPV 135
           ++YRDH   + +G    +  SEL G+ TGC  G+GGSMH + K+ +  GG   +G  IP+
Sbjct: 119 TSYRDHCTFIGRGGKLVDAFSELMGRKTGCSHGKGGSMHFYKKDASFYGGHGIVGAQIPL 178

Query: 136 ATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLNMAALWKLPIVFVVENNL 195
             G AF  KY ++       E VT A +GDG  N GQ FE LN++ALW LP + V ENN 
Sbjct: 179 GCGLAFAQKYNKD-------EAVTFALYGDGAANQGQLFEALNISALWDLPAILVCENNH 238

Query: 196 WAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGEGPTLVEC 255
           + +G +  R+   P  +K+G    +PG+ VDGMD L V++  K A   A +  GP ++E 
Sbjct: 239 YGMGTATWRSAKSPAYFKRGDY--VPGLKVDGMDALAVKQACKFAKEHALK-NGPIILEM 298

Query: 256 ETYRFRGHSLADPD-------------ELRDPVNIVKGRNQSNLMDITTPLCDFYMIAHK 315
           +TYR+ GHS++DP              ++RDP+  V+    ++ +     L D      K
Sbjct: 299 DTYRYHGHSMSDPGSTYRTRDEISGVRQVRDPIERVRKLLLTHDIATEKELKDMEKEIRK 358

Query: 316 EKHLELIKYKGLRIPD 318
           E    + + K   IPD
Sbjct: 359 EVDDAVAQAKESPIPD 363

[Close]
BLAST of Lsi09G017150 vs. TAIR10
Match: AT5G09300.1 (AT5G09300.1 Thiamin diphosphate-binding fold (THDP-binding) superfamily protein)

HSP 1 Score: 114.8 bits (286), Expect = 1.3e-25
Identity = 69/252 (27.38%), Postives = 121/252 (48.02%), Query Frame = 1 

Query: 15  ITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLTKRDTVV 74
           +++E  + +Y DM+  +  +++  +   +G++  F     G+EA++      LT +D + 
Sbjct: 125 VSEEVAVKIYSDMVTLQIMDNIFYEAQRQGRL-SFYATAIGEEAINIASAAALTPQDVIF 184

Query: 75  STYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSKEHNLIGGFAFIGEGIPV 134
             YR+    L +G   +E  ++ FG  +   +G+   +H  S + N     A I   +P 
Sbjct: 185 PQYREPGVLLWRGFTLQEFANQCFGNKSDYGKGRQMPVHYGSNKLNYFTVSATIATQLPN 244

Query: 135 ATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLNMAALWKLPIVFVVENNL 194
           A GAA++ K  ++       +   + +FGDG  + G F   LN+AA+ + P++F+  NN 
Sbjct: 245 AVGAAYSLKMDKK-------DACAVTYFGDGGTSEGDFHAALNIAAVMEAPVLFICRNNG 304

Query: 195 WAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGEGPTLVEC 254
           WAI            +  KG A+G+  + VDG D L +      A   A R + P L+E 
Sbjct: 305 WAISTPTSDQFRSDGVVVKGRAYGIRSIRVDGNDALAMYSAVHTAREMAIREQRPILIEA 364

Query: 255 ETYRFRGHSLAD 267
            TYR   HS +D
Sbjct: 365 LTYRVGHHSTSD 368

[Close]
BLAST of Lsi09G017150 vs. TAIR10
Match: AT1G21400.1 (AT1G21400.1 Thiamin diphosphate-binding fold (THDP-binding) superfamily protein)

HSP 1 Score: 103.6 bits (257), Expect = 3.0e-22
Identity = 70/260 (26.92%), Postives = 110/260 (42.31%), Query Frame = 1 

Query: 15  ITKEEGLVLYEDMILGREFEDMCA-----QMYYRGKMFGFVHLYN---GQEAVSTGFIKL 74
           I  +   +   + +  R +E M        ++Y  +  G +  Y    G+EA++      
Sbjct: 116 IIPDSDFIPVSEKLAVRMYEQMATLQVMDHIFYEAQRQGRISFYLTSVGEEAINIASAAA 175

Query: 75  LTKRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSKEHNLIGGFA 134
           L+  D V+  YR+    L +G    E  ++ FG      +G+   +H  S   N     +
Sbjct: 176 LSPDDVVLPQYREPGVLLWRGFTLEEFANQCFGNKADYGKGRQMPIHYGSNRLNYFTISS 235

Query: 135 FIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLNMAALWKLPI 194
            I   +P A G  ++ K  ++          T+ F GDG  + G F   LN AA+ + P+
Sbjct: 236 PIATQLPQAAGVGYSLKMDKK-------NACTVTFIGDGGTSEGDFHAGLNFAAVMEAPV 295

Query: 195 VFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRG 254
           VF+  NN WAI            I  KG A+G+  + VDG D L V    + A   A   
Sbjct: 296 VFICRNNGWAISTHISEQFRSDGIVVKGQAYGIRSIRVDGNDALAVYSAVRSAREMAVTE 355

Query: 255 EGPTLVECETYRFRGHSLAD 267
           + P L+E  TYR   HS +D
Sbjct: 356 QRPVLIEMMTYRVGHHSTSD 368

[Close]
BLAST of Lsi09G017150 vs. NCBI nr
Match: gi|449445539|ref|XP_004140530.1| (PREDICTED: pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic [Cucumis sativus])

HSP 1 Score: 548.1 bits (1411), Expect = 1.3e-152
Identity = 276/336 (82.14%), Postives = 291/336 (86.61%), Query Frame = 1 

Query: 14  LITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLTKRDTV 73
           LITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLT+RDTV
Sbjct: 77  LITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLTQRDTV 136

Query: 74  VSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSKEHNLIGGFAFIGEGIP 133
           VSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSKEHNLIGGFAFIGEGIP
Sbjct: 137 VSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSKEHNLIGGFAFIGEGIP 196

Query: 134 VATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLNMAALWKLPIVFVVENN 193
           VATGAAFTSKYKREVLKEADC+DVTLAFFGDGTCNNGQFFECLNMAALWKLPIVFVVENN
Sbjct: 197 VATGAAFTSKYKREVLKEADCQDVTLAFFGDGTCNNGQFFECLNMAALWKLPIVFVVENN 256

Query: 194 LWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGEGPTLVE 253
           LWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGEGPTLVE
Sbjct: 257 LWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGEGPTLVE 316

Query: 254 CETYRFRGHSLADPDELRDPVNIVKGRNQSNLMDITTPLCDFYMIAHK---EKHLELIKY 313
           CETYRFRGHSLADPDELRDP    +   +  +  +       YM+ +K   E+ L+ IK 
Sbjct: 317 CETYRFRGHSLADPDELRDPDEKARYAARDPIAALKK-----YMLENKLANEQELKAIKD 376

Query: 314 KGLRIPDLTVADEKARYAARDPIAALKKYLLENNLA 347
           K      + V +E  ++A   P  A +  LLEN  A
Sbjct: 377 K-----IVEVVEEAVQFADESPHPA-RSQLLENVFA 401

[Close]
BLAST of Lsi09G017150 vs. NCBI nr
Match: gi|659119761|ref|XP_008459830.1| (PREDICTED: pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic [Cucumis melo])

HSP 1 Score: 545.4 bits (1404), Expect = 8.4e-152
Identity = 272/336 (80.95%), Postives = 291/336 (86.61%), Query Frame = 1 

Query: 14  LITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLTKRDTV 73
           LITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLT+RDTV
Sbjct: 77  LITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLTQRDTV 136

Query: 74  VSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSKEHNLIGGFAFIGEGIP 133
           VSTYRDHVHA+SKGVPSR+VMSELFGKTTGCCRGQGGSMHMFSKEHNLIGGFAFIGEGIP
Sbjct: 137 VSTYRDHVHAISKGVPSRQVMSELFGKTTGCCRGQGGSMHMFSKEHNLIGGFAFIGEGIP 196

Query: 134 VATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLNMAALWKLPIVFVVENN 193
           VATGAAFTSKYKREVLKE DCEDVTLAFFGDGTCNNGQFFECLNMAALWKLPIVFVVENN
Sbjct: 197 VATGAAFTSKYKREVLKETDCEDVTLAFFGDGTCNNGQFFECLNMAALWKLPIVFVVENN 256

Query: 194 LWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGEGPTLVE 253
           LWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGEGPTLVE
Sbjct: 257 LWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGEGPTLVE 316

Query: 254 CETYRFRGHSLADPDELRDPVNIVKGRNQSNLMDITTPLCDFYMIAHK---EKHLELIKY 313
           CETYRFRGHSLADPDELRDP    +   +  +  +       Y++ +K   E+ L+ IK 
Sbjct: 317 CETYRFRGHSLADPDELRDPDEKARYAARDPIAALKK-----YLLENKLANEQELKAIKD 376

Query: 314 KGLRIPDLTVADEKARYAARDPIAALKKYLLENNLA 347
           K      + V ++  ++A   P+ A +  LLEN  A
Sbjct: 377 K-----IVEVVEDAVQFADESPLPA-RSQLLENVFA 401

[Close]
BLAST of Lsi09G017150 vs. NCBI nr
Match: gi|698495681|ref|XP_009793937.1| (PREDICTED: pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic-like [Nicotiana sylvestris])

HSP 1 Score: 530.8 bits (1366), Expect = 2.1e-147
Identity = 262/333 (78.68%), Postives = 281/333 (84.38%), Query Frame = 1 

Query: 14  LITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLTKRDTV 73
           LITKEEGLVLYEDM+LGR FEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLL K D+V
Sbjct: 83  LITKEEGLVLYEDMVLGRAFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLKKEDSV 142

Query: 74  VSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSKEHNLIGGFAFIGEGIP 133
           VSTYRDHVHALSKGVP+R+VMSELFGKTTGCCRGQGGSMHMFSKEHN++GGFAFIGEGIP
Sbjct: 143 VSTYRDHVHALSKGVPARQVMSELFGKTTGCCRGQGGSMHMFSKEHNVLGGFAFIGEGIP 202

Query: 134 VATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLNMAALWKLPIVFVVENN 193
           VATGAAFTSKY+REVLKEADC+ VTLAFFGDGTCNNGQF+ECLNMAALWKLPI+FVVENN
Sbjct: 203 VATGAAFTSKYRREVLKEADCDHVTLAFFGDGTCNNGQFYECLNMAALWKLPIIFVVENN 262

Query: 194 LWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGEGPTLVE 253
           LWAIGMSHLR+TSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGEGPTLVE
Sbjct: 263 LWAIGMSHLRSTSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGEGPTLVE 322

Query: 254 CETYRFRGHSLADPDELRDPVNIVKGRNQSNLMDITTPLCDFYMIAHKEKHLELIKYKGL 313
           CETYRFRGHSLADPDELRDP      +N     D  T L  +    +     EL   K +
Sbjct: 323 CETYRFRGHSLADPDELRDPAE----KNHYATRDPITALKKYMFENNLVSEAEL---KAI 382

Query: 314 RIPDLTVADEKARYAARDPIAALKKYLLENNLA 347
                 V +E   +A   P+ A +  LLEN  A
Sbjct: 383 DKKIDEVVEESVEFADASPVPA-RSQLLENVFA 407

[Close]
BLAST of Lsi09G017150 vs. NCBI nr
Match: gi|697163275|ref|XP_009590458.1| (PREDICTED: pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic-like [Nicotiana tomentosiformis])

HSP 1 Score: 530.4 bits (1365), Expect = 2.8e-147
Identity = 263/333 (78.98%), Postives = 283/333 (84.98%), Query Frame = 1 

Query: 14  LITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLTKRDTV 73
           LITKEEGLVLYEDM+LGR FEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLL K D+V
Sbjct: 83  LITKEEGLVLYEDMVLGRAFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLKKEDSV 142

Query: 74  VSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSKEHNLIGGFAFIGEGIP 133
           VSTYRDHVHALSKGVP+R+VMSELFGKTTGCCRGQGGSMHMFSKEHN++GGFAFIGEGIP
Sbjct: 143 VSTYRDHVHALSKGVPARQVMSELFGKTTGCCRGQGGSMHMFSKEHNVLGGFAFIGEGIP 202

Query: 134 VATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLNMAALWKLPIVFVVENN 193
           VATGAAFTSKY+REVLKEADC+ VTLAFFGDGTCNNGQF+ECLNMAALWKLPI+FVVENN
Sbjct: 203 VATGAAFTSKYRREVLKEADCDHVTLAFFGDGTCNNGQFYECLNMAALWKLPIIFVVENN 262

Query: 194 LWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGEGPTLVE 253
           LWAIGMSHLR+TSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGEGPTLVE
Sbjct: 263 LWAIGMSHLRSTSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGEGPTLVE 322

Query: 254 CETYRFRGHSLADPDELRDPVNIVKGRNQSNLMDITTPLCDFYMIAHKEKHLELIKYKGL 313
           CETYRFRGHSLADPDELRDP      +N     D  T L  +    +     EL K    
Sbjct: 323 CETYRFRGHSLADPDELRDPAE----KNHYATRDPITALKKYMFENNLVSEAEL-KAIDK 382

Query: 314 RIPDLTVADEKARYAARDPIAALKKYLLENNLA 347
           +I +L   +E   +A   P+ A +  LLEN  A
Sbjct: 383 KIDEL--VEESVEFADASPVPA-RSQLLENVFA 407

[Close]
BLAST of Lsi09G017150 vs. NCBI nr
Match: gi|565374375|ref|XP_006353739.1| (PREDICTED: pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic-like [Solanum tuberosum])

HSP 1 Score: 528.5 bits (1360), Expect = 1.1e-146
Identity = 261/333 (78.38%), Postives = 283/333 (84.98%), Query Frame = 1 

Query: 14  LITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLTKRDTV 73
           LITKEEGLVLYEDM+LGR FEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLL K D+V
Sbjct: 86  LITKEEGLVLYEDMVLGRTFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLKKEDSV 145

Query: 74  VSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSKEHNLIGGFAFIGEGIP 133
           VSTYRDHVHALSKGVP+R+VMSELFGKTTGCCRGQGGSMHMFSKEHN++GGFAFIGEGIP
Sbjct: 146 VSTYRDHVHALSKGVPARQVMSELFGKTTGCCRGQGGSMHMFSKEHNVLGGFAFIGEGIP 205

Query: 134 VATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLNMAALWKLPIVFVVENN 193
           VATGAAFTSKY+REVLKEADC+ VTLAFFGDGTCNNGQF+ECLNMAALWKLPI+FVVENN
Sbjct: 206 VATGAAFTSKYRREVLKEADCDHVTLAFFGDGTCNNGQFYECLNMAALWKLPIIFVVENN 265

Query: 194 LWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGEGPTLVE 253
           LWAIGMSHLR+TSDPEIWKKGPAFGMPGVHVDGMDVLKVREVA EA+GRARRGEGPTLVE
Sbjct: 266 LWAIGMSHLRSTSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAMEAVGRARRGEGPTLVE 325

Query: 254 CETYRFRGHSLADPDELRDPVNIVKGRNQSNLMDITTPLCDFYMIAHKEKHLELIKYKGL 313
           CETYRFRGHSLADPDELRDP      +N     D  T L   YM  +   +   +K    
Sbjct: 326 CETYRFRGHSLADPDELRDPAE----KNHYATRDPITAL-KKYMFENNLVNEAELKAIDK 385

Query: 314 RIPDLTVADEKARYAARDPIAALKKYLLENNLA 347
           +I +L   +E   +A   P+ A +  LLEN  A
Sbjct: 386 KIDEL--VEESVEFAEASPVPA-RNQLLENVFA 410

The following BLAST results are available for this feature:
BLAST of Lsi09G017150 vs. Swiss-Prot
Match NameE-valueIdentityDescription
ODPA3_ARATH2.1e-14276.88Pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic OS=Arabidopsi... [more]
ODPA3_ORYSJ3.2e-13588.12Pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic OS=Oryza sati... [more]
ODPA_PORPU4.1e-11461.88Pyruvate dehydrogenase E1 component subunit alpha OS=Porphyra purpurea GN=pdhA P... [more]
ODPA_PYRYE7.7e-11374.71Pyruvate dehydrogenase E1 component subunit alpha OS=Pyropia yezoensis GN=pdhA P... [more]
ODPA_RHIME7.0e-6644.94Pyruvate dehydrogenase E1 component subunit alpha OS=Rhizobium meliloti (strain ... [more]
BLAST of Lsi09G017150 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KFF3_CUCSA9.0e-15382.14Pyruvate dehydrogenase E1 component subunit alpha OS=Cucumis sativus GN=Csa_6G09... [more]
M1B234_SOLTU7.4e-14778.38Pyruvate dehydrogenase E1 component subunit alpha OS=Solanum tuberosum GN=PGSC00... [more]
B5LAW2_CAPAN3.7e-14678.08Pyruvate dehydrogenase E1 component subunit alpha OS=Capsicum annuum PE=2 SV=1[more]
A0A068UJA1_COFCA2.4e-14577.48Pyruvate dehydrogenase E1 component subunit alpha OS=Coffea canephora GN=GSCOC_T... [more]
A9PF50_POPTR6.9e-14594.62Pyruvate dehydrogenase E1 component subunit alpha OS=Populus trichocarpa GN=POPT... [more]
BLAST of Lsi09G017150 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G01090.11.2e-14376.88 pyruvate dehydrogenase E1 alpha[more]
AT1G59900.11.1e-5637.97 pyruvate dehydrogenase complex E1 alpha subunit[more]
AT1G24180.17.8e-5536.39 Thiamin diphosphate-binding fold (THDP-binding) superfamily protein[more]
AT5G09300.11.3e-2527.38 Thiamin diphosphate-binding fold (THDP-binding) superfamily protein[more]
AT1G21400.13.0e-2226.92 Thiamin diphosphate-binding fold (THDP-binding) superfamily protein[more]
BLAST of Lsi09G017150 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449445539|ref|XP_004140530.1|1.3e-15282.14PREDICTED: pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic [C... [more]
gi|659119761|ref|XP_008459830.1|8.4e-15280.95PREDICTED: pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic [C... [more]
gi|698495681|ref|XP_009793937.1|2.1e-14778.68PREDICTED: pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic-li... [more]
gi|697163275|ref|XP_009590458.1|2.8e-14778.98PREDICTED: pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic-li... [more]
gi|565374375|ref|XP_006353739.1|1.1e-14678.38PREDICTED: pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic-li... [more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Cellular Component
TermDefinition
GO:0043231intracellular membrane-bounded organelle
Vocabulary: Biological Process
TermDefinition
GO:0006086acetyl-CoA biosynthetic process from pyruvate
GO:0008152metabolic process
Vocabulary: Molecular Function
TermDefinition
GO:0004739pyruvate dehydrogenase (acetyl-transferring) activity
GO:0016624oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor
Vocabulary: INTERPRO
TermDefinition
IPR017597Pyrv_DH_E1_asu_subgrp-y
IPR001017DH_E1
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006086 acetyl-CoA biosynthetic process from pyruvate
biological_process GO:0006094 gluconeogenesis
biological_process GO:0006096 glycolytic process
biological_process GO:0009097 isoleucine biosynthetic process
biological_process GO:0009098 leucine biosynthetic process
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0009099 valine biosynthetic process
biological_process GO:0008152 metabolic process
cellular_component GO:0009941 chloroplast envelope
cellular_component GO:0009570 chloroplast stroma
cellular_component GO:0045254 pyruvate dehydrogenase complex
cellular_component GO:0043231 intracellular membrane-bounded organelle
molecular_function GO:0004739 pyruvate dehydrogenase (acetyl-transferring) activity
molecular_function GO:0016624 oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi09G017150.1Lsi09G017150.1mRNA


InterPro
Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001017Dehydrogenase, E1 componentPFAMPF00676E1_dhcoord: 327..375
score: 2.3E-5coord: 26..269
score: 3.0
IPR017597Pyruvate dehydrogenase (acetyl-transferring) E1 component, alpha subunit, subgroup yTIGRFAMsTIGR03182TIGR03182coord: 18..298
score: 2.3E
NoneNo IPR availablePANTHERPTHR11516PYRUVATE DEHYDROGENASE E1 COMPONENT, ALPHA SUBUNIT BACTERIAL AND ORGANELLARcoord: 322..409
score: 1.1E-230coord: 10..273
score: 1.1E
NoneNo IPR availablePANTHERPTHR11516:SF25PYRUVATE DEHYDROGENASE E1 COMPONENT SUBUNIT ALPHA-3, CHLOROPLASTICcoord: 322..409
score: 1.1E-230coord: 10..273
score: 1.1E